AT3G27430

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002814
Physical Location & Seq
Forward (+)
10152458 .. 10155447
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27430.3

Sequence Viewer

Length: 804 bp
ATGTCGCAATCAACCGTAGATGTTCCGCCAAAGGGTGGGTTTAGCTTCGATCTGTGTAAGAGAAATGATATGCTTACACAAAAGGGTCTTAAAGCTCCGTCTTTTTTGAAGACTGGAACAACCATTGTTGGTTTGATTTTCAAGGATGGTGTGATACTAGGGGCAGATACCCGAGCAACTGAGGGGCCAATTGTTGCTGATAAGAACTGTGAGAAGATTCACTATATGGCACCAAACATATATTGCTGTGGTGCAGGAACTGCGGCTGATACTGAAGCAGTCACTGATATGGTCAGCTCACAGCTGCGATTGCATCGTTACCAGACTGGTCGAGACTCTCGGGTCATTACTGCTTTGACCCTTCTCAAAAAACATCTTTTCAGCTACCAAGGTCATGTCTCTGCTGCTCTTGTACTCGGTGGAGTTGATATCACTGGTCCACATCTGCATACTATATACCCACACGGTTCAACTGACACTCTTCCATTCGCCACAATGGGTTCGGGTTCTCTTGCTGCTATGTCTGTGTTTGAGGCAAAGTATAAAGAAGGCCTAACTAGGGATGAAGGAATTAAGCTGGTCGCTGAATCCATATGCTCGGGTATATTCAATGACCTGGGTAGTGGTAGCAACGTGGACATCTGCGTGATCACAAAGGGGAACAAGGAATATCTCAGGAACTACATGGAACCAAACCCAAGAACCTATGTCAGCAGCAAAGGCTATTCCTTCACCAAGAAAACTGGTTCTTCTCACCAAAATCACCCCATTGCTGGAGCGAGTCGAAATCACAGAAGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

267

Amino Acids

28.81

Weight (kDa)

8.94

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 39 - 211 1.7e-43 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 229
AccB7I CCANNNNNTGG 1 cut(s) 35
AciI CCGC 2 cut(s) 26, 263
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 414
AfiI CCNNNNNNNGG 4 cut(s) 32, 35, 559, 773
AgsI TTSAA 4 cut(s) 109, 142, 471, 610
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 6 cut(s) 45, 95, 297, 304, 384, 577
AluI AGCT 6 cut(s) 45, 95, 297, 304, 384, 577
Alw26I GTCTC 2 cut(s) 327, 403
AlwNI CAGNNNCTG 2 cut(s) 260, 284
Ama87I CYCGRG 3 cut(s) 171, 339, 598
AoxI GGCC 2 cut(s) 185, 550
ApeKI GCWGC 4 cut(s) 304, 404, 515, 714
AspS9I GGNCC 2 cut(s) 185, 437
AsuHPI GGTGA 3 cut(s) 724, 746, 755
AvaI CYCGRG 3 cut(s) 171, 339, 598
AvaII GGWCC 1 cut(s) 437
BanI GGYRCC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 116
BbvI GCAGC 4 cut(s) 291, 391, 502, 726
BccI CCATC 1 cut(s) 140
BciT130I CCWGG 1 cut(s) 617
BclI TGATCA 1 cut(s) 648
BcoDI GTCTC 2 cut(s) 327, 403
BfaI CTAG 2 cut(s) 158, 558
BisI GCNGC 5 cut(s) 264, 305, 405, 516, 715
BlsI GCNGC 5 cut(s) 265, 306, 406, 517, 716
Bme1390I CCNGG 1 cut(s) 617
Bme18I GGWCC 1 cut(s) 437
BmeT110I CYCGRG 3 cut(s) 171, 339, 598
BmgT120I GGNCC 2 cut(s) 185, 437
BmiI GGNNCC 3 cut(s) 186, 231, 690
BmrFI CCNGG 1 cut(s) 617
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 795
BsaJI CCNNGG 2 cut(s) 388, 616
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 35, 559, 773
Bse1I ACTGG 4 cut(s) 118, 331, 439, 748
Bse3DI GCAATG 1 cut(s) 768
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 2 cut(s) 388, 616
BseGI GGATG 2 cut(s) 151, 568
BseLI CCNNNNNNNGG 4 cut(s) 32, 35, 559, 773
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 2 cut(s) 171, 688
BseNI ACTGG 4 cut(s) 118, 331, 439, 748
BseXI GCAGC 4 cut(s) 291, 391, 502, 726
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 2 cut(s) 187, 552
BshNI GGYRCC 1 cut(s) 229
BsiHKCI CYCGRG 3 cut(s) 171, 339, 598
BslI CCNNNNNNNGG 4 cut(s) 32, 35, 559, 773
BsmAI GTCTC 2 cut(s) 327, 403
BsnI GGCC 2 cut(s) 187, 552
BsoBI CYCGRG 3 cut(s) 171, 339, 598
Bsp143I GATC 2 cut(s) 49, 648
BspACI CCGC 2 cut(s) 26, 263
BspANI GGCC 2 cut(s) 187, 552
BspCNI CTCAG 2 cut(s) 172, 687
BspLI GGNNCC 3 cut(s) 186, 231, 690
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 768
BsrI ACTGG 4 cut(s) 118, 331, 439, 748
BssECI CCNNGG 2 cut(s) 388, 616
BssMI GATC 2 cut(s) 49, 648
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 3 cut(s) 16, 209, 467
Bst6I CTCTTC 1 cut(s) 486
BstAPI GCANNNNNTGC 1 cut(s) 260
BstDEI CTNAG 2 cut(s) 180, 674
BstF5I GGATG 2 cut(s) 151, 568
BstKTI GATC 2 cut(s) 52, 651
BstMAI GTCTC 2 cut(s) 327, 403
BstMBI GATC 2 cut(s) 49, 648
BstMWI GCNNNNNNNGC 3 cut(s) 260, 310, 720
BstNI CCWGG 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 615
BstV1I GCAGC 4 cut(s) 291, 391, 502, 726
BstV2I GAAGAC 1 cut(s) 116
BsuRI GGCC 2 cut(s) 187, 552
BtsCI GGATG 2 cut(s) 151, 568
BtsIMutI CAGTG 2 cut(s) 282, 432
CaiI CAGNNNCTG 2 cut(s) 260, 284
Cfr13I GGNCC 2 cut(s) 185, 437
Csp6I GTAC 1 cut(s) 413
CviAII CATG 2 cut(s) 395, 685
CviQI GTAC 1 cut(s) 413
DdeI CTNAG 2 cut(s) 180, 674
DpnI GATC 2 cut(s) 51, 650
DpnII GATC 2 cut(s) 49, 648
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
EciI GGCGGA 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 388
Eco147I AGGCCT 1 cut(s) 552
Eco32I GATATC 1 cut(s) 430
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 3 cut(s) 171, 339, 598
EcoRII CCWGG 1 cut(s) 615
EcoRV GATATC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 2 cut(s) 398, 688
FatI CATG 2 cut(s) 394, 684
FauNDI CATATG 1 cut(s) 593
FbaI TGATCA 1 cut(s) 648
Fnu4HI GCNGC 5 cut(s) 264, 305, 405, 516, 715
FokI GGATG 2 cut(s) 158, 575
Fsp4HI GCNGC 5 cut(s) 264, 305, 405, 516, 715
FspBI CTAG 2 cut(s) 158, 558
GluI GCNGC 5 cut(s) 264, 305, 405, 516, 715
GsuI CTGGAG 1 cut(s) 795
HaeIII GGCC 2 cut(s) 187, 552
Hin1II CATG 2 cut(s) 398, 688
HinfI GANTC 4 cut(s) 217, 335, 587, 781
HphI GGTGA 3 cut(s) 724, 746, 755
Hpy166II GTNNAC 2 cut(s) 440, 637
Hpy188III TCNNGA 2 cut(s) 332, 676
Hpy8I GTNNAC 2 cut(s) 440, 637
HpyAV CCTTC 4 cut(s) 371, 542, 560, 739
HpyCH4III ACNGT 3 cut(s) 16, 209, 467
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 3 cut(s) 254, 313, 448
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 310, 720
HpyF3I CTNAG 2 cut(s) 180, 674
HpySE526I ACGT 1 cut(s) 633
Hsp92II CATG 2 cut(s) 398, 688
Ksp22I TGATCA 1 cut(s) 648
Kzo9I GATC 2 cut(s) 49, 648
LmnI GCTCC 2 cut(s) 100, 776
Lsp1109I GCAGC 4 cut(s) 291, 391, 502, 726
LweI GCATC 1 cut(s) 322
MaeI CTAG 2 cut(s) 158, 558
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 2 cut(s) 280, 317
MalI GATC 2 cut(s) 51, 650
MboI GATC 2 cut(s) 49, 648
MboII GAAGA 4 cut(s) 121, 226, 473, 741
MfeI CAATTG 1 cut(s) 189
MluCI AATT 2 cut(s) 189, 570
MlyI GAGTC 2 cut(s) 329, 790
MnlI CCTC 2 cut(s) 175, 526
MseI TTAA 2 cut(s) 90, 573
MslI CAYNNNNRTG 2 cut(s) 287, 644
MspA1I CMGCKG 1 cut(s) 304
MspR9I CCNGG 1 cut(s) 617
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 617
MwoI GCNNNNNNNGC 3 cut(s) 260, 310, 720
NdeI CATATG 1 cut(s) 593
NdeII GATC 2 cut(s) 49, 648
NlaIII CATG 2 cut(s) 398, 688
NlaIV GGNNCC 3 cut(s) 186, 231, 690
NmuCI GTSAC 1 cut(s) 280
PceI AGGCCT 1 cut(s) 552
PfeI GAWTC 2 cut(s) 217, 587
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 5 cut(s) 265, 306, 406, 517, 716
PleI GAGTC 2 cut(s) 329, 789
PpsI GAGTC 2 cut(s) 329, 789
Psp6I CCWGG 1 cut(s) 615
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 3 cut(s) 186, 231, 690
PspPI GGNCC 2 cut(s) 185, 437
PstNI CAGNNNCTG 2 cut(s) 260, 284
PvuII CAGCTG 1 cut(s) 304
RsaI GTAC 1 cut(s) 414
RsaNI GTAC 1 cut(s) 413
RseI CAYNNNNRTG 2 cut(s) 287, 644
SaqAI TTAA 2 cut(s) 90, 573
SatI GCNGC 5 cut(s) 264, 305, 405, 516, 715
Sau3AI GATC 2 cut(s) 49, 648
Sau96I GGNCC 2 cut(s) 185, 437
SchI GAGTC 2 cut(s) 329, 790
ScrFI CCNGG 1 cut(s) 617
SfaNI GCATC 1 cut(s) 322
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 2 cut(s) 287, 644
Sse9I AATT 2 cut(s) 189, 570
SseBI AGGCCT 1 cut(s) 552
SsiI CCGC 2 cut(s) 26, 263
SspMI CTAG 2 cut(s) 158, 558
StuI AGGCCT 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 615
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 3 cut(s) 16, 209, 467
TaiI ACGT 1 cut(s) 636
TaqI TCGA 3 cut(s) 48, 331, 784
TasI AATT 2 cut(s) 189, 570
TatI WGTACW 1 cut(s) 412
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 2 cut(s) 217, 587
Tru1I TTAA 2 cut(s) 90, 573
Tru9I TTAA 2 cut(s) 90, 573
TscAI CASTG 2 cut(s) 289, 439
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 4 cut(s) 304, 404, 515, 714
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 579
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 2 cut(s) 289, 439
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 437
XspI CTAG 2 cut(s) 158, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.