MD09G1020800.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002814
Physical Location & Seq
Forward (+)
1266243 .. 1269201
2959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1020800.v1.1.491

Sequence Viewer

Length: 819 bp
ATGTCTCAGGTAGCGGCAGATGTTCCCAAGGGTGGGTTCAGTTTTGATCTTTGTCGAAGAAACGACATGCTTGCAAAGAATGGAGTTCAGCAGCCATCTTATAGGAAGACGGGAACCACCATTGTTGGTTTGATTTTTAAGGATGGTGTCATTCTTGGAGCTGATACAAGGGCAACTGAAGGACCCATTGTGTGTGATAAGAACTGTGAAAAGATCCATTATATGGCACCCAATATCTATTGCTGTGGAGCTGGAACTGCTGCTGATACAGAGGCTGTAACAGATATGGTGAGCTCACAGCTGCAACTGCATCGCTACCATACTGGTCGTGAATCTCGGGTTGTGACAGCCTTGACCTTGCTCAAGAAACACCTTTTCAATTACCAAGGTCATGTGTCAGCTGCTTTGGTGCTCGGGGGTGTTGATGTCACTGGACCCCATCTGCATACTATATATCCCCATGGATCAACTGACACACTGCCATTTGCTACAATGGGTTCTGGTTCTCTTGCCGCGATGGCCATGTTTGAGTCCAAGTACAAAGAAGGCATGACTAGGGATGAAGGCATAAAGCTTGTAACTGAAGCAATATGCTCTGGAATTTTCAATGACTTGGGAAGTGGAAGCAATGTTGATGTTTGTGTAATAACTAAGGGACACAAGGAATACTTGAGGAACCACTTGGTGCCGACCCCTCGTACCTATGTCAGTGCCGAAGGCTATAAGTTTACCAAGAAGACCGAGGTTCTCTTCACAAAGATCACTCCATTGACTAAGAAGGCAGAAGTGATTGAAGGAGGCGATGCAATGGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

273

Amino Acids

29.44

Weight (kDa)

7.13

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 36 - 216 8.5e-51 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 226, 685
AccB7I CCANNNNNTGG 1 cut(s) 223
AccII CGCG 1 cut(s) 515
AciI CCGC 2 cut(s) 14, 513
AclWI GGATC 2 cut(s) 208, 472
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 1 cut(s) 600
AcuI CTGAAG 2 cut(s) 198, 603
AdeI CACNNNGTG 1 cut(s) 685
AfaI GTAC 2 cut(s) 539, 700
AfiI CCNNNNNNNGG 3 cut(s) 32, 33, 223
AgsI TTSAA 3 cut(s) 379, 607, 794
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
AluBI AGCT 6 cut(s) 161, 251, 294, 301, 401, 574
AluI AGCT 6 cut(s) 161, 251, 294, 301, 401, 574
Alw21I GWGCWC 2 cut(s) 296, 414
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 208, 472
AlwNI CAGNNNCTG 1 cut(s) 275
Ama87I CYCGRG 2 cut(s) 336, 413
AoxI GGCC 1 cut(s) 519
ApeKI GCWGC 4 cut(s) 91, 260, 301, 401
ApoI RAATTY 1 cut(s) 600
AspS9I GGNCC 2 cut(s) 182, 434
AsuHPI GGTGA 1 cut(s) 301
AvaI CYCGRG 2 cut(s) 336, 413
AvaII GGWCC 2 cut(s) 182, 434
BalI TGGCCA 1 cut(s) 521
BanI GGYRCC 2 cut(s) 226, 685
BanII GRGCYC 1 cut(s) 296
BbsI GAAGAC 2 cut(s) 113, 743
Bbv12I GWGCWC 2 cut(s) 296, 414
BbvI GCAGC 4 cut(s) 103, 247, 288, 388
BccI CCATC 4 cut(s) 103, 137, 447, 511
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 293, 327
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 555
BglI GCCNNNNNGGC 1 cut(s) 518
BisI GCNGC 6 cut(s) 15, 92, 261, 302, 402, 513
BlsI GCNGC 6 cut(s) 16, 93, 262, 303, 403, 514
Bme18I GGWCC 2 cut(s) 182, 434
BmeT110I CYCGRG 2 cut(s) 336, 413
BmgT120I GGNCC 2 cut(s) 182, 434
BmiI GGNNCC 6 cut(s) 115, 184, 228, 436, 677, 687
BmsI GCATC 2 cut(s) 319, 793
BpiI GAAGAC 2 cut(s) 113, 743
BpuEI CTTGAG 2 cut(s) 347, 691
BsaJI CCNNGG 4 cut(s) 27, 385, 460, 741
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 33, 223
Bse1I ACTGG 2 cut(s) 328, 436
Bse3DI GCAATG 2 cut(s) 634, 813
BseDI CCNNGG 4 cut(s) 27, 385, 460, 741
BseGI GGATG 2 cut(s) 148, 565
BseLI CCNNNNNNNGG 3 cut(s) 32, 33, 223
BseMI GCAATG 2 cut(s) 634, 813
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 328, 436
BseXI GCAGC 4 cut(s) 103, 247, 288, 388
Bsh1236I CGCG 1 cut(s) 515
BshFI GGCC 1 cut(s) 521
BshNI GGYRCC 2 cut(s) 226, 685
BsiHKAI GWGCWC 2 cut(s) 296, 414
BsiHKCI CYCGRG 2 cut(s) 336, 413
BslFI GGGAC 1 cut(s) 669
BslI CCNNNNNNNGG 3 cut(s) 32, 33, 223
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 669
BsnI GGCC 1 cut(s) 521
BsoBI CYCGRG 2 cut(s) 336, 413
Bsp1286I GDGCHC 2 cut(s) 296, 414
Bsp143I GATC 4 cut(s) 46, 213, 464, 759
Bsp19I CCATGG 1 cut(s) 460
BspACI CCGC 2 cut(s) 14, 513
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 1 cut(s) 515
BspLI GGNNCC 6 cut(s) 115, 184, 228, 436, 677, 687
BspPI GGATC 2 cut(s) 208, 472
BspT107I GGYRCC 2 cut(s) 226, 685
BsrDI GCAATG 2 cut(s) 634, 813
BsrI ACTGG 2 cut(s) 328, 436
BssECI CCNNGG 4 cut(s) 27, 385, 460, 741
BssMI GATC 4 cut(s) 46, 213, 464, 759
BssT1I CCWWGG 3 cut(s) 27, 385, 460
Bst4CI ACNGT 1 cut(s) 206
Bst6I CTCTTC 2 cut(s) 755, 807
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 3 cut(s) 6, 651, 774
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 2 cut(s) 148, 565
BstFNI CGCG 1 cut(s) 515
BstKTI GATC 4 cut(s) 49, 216, 467, 762
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 46, 213, 464, 759
BstMWI GCNNNNNNNGC 3 cut(s) 257, 307, 518
BstNSI RCATGY 1 cut(s) 70
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 4 cut(s) 103, 247, 288, 388
BstV2I GAAGAC 2 cut(s) 113, 743
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 1 cut(s) 521
BtgI CCRYGG 1 cut(s) 460
BtgZI GCGATG 2 cut(s) 296, 530
BtsCI GGATG 2 cut(s) 148, 565
BtsI GCAGTG 1 cut(s) 476
BtsIMutI CAGTG 3 cut(s) 429, 476, 715
Cac8I GCNNGC 1 cut(s) 72
CaiI CAGNNNCTG 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 182, 434
Csp6I GTAC 2 cut(s) 538, 699
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 5 cut(s) 67, 392, 461, 523, 550
CviQI GTAC 2 cut(s) 538, 699
DdeI CTNAG 3 cut(s) 6, 651, 774
DpnI GATC 4 cut(s) 48, 215, 466, 761
DpnII GATC 4 cut(s) 46, 213, 464, 759
DraIII CACNNNGTG 1 cut(s) 685
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 755, 807
EarI CTCTTC 2 cut(s) 755, 807
Ecl136II GAGCTC 1 cut(s) 294
Eco130I CCWWGG 3 cut(s) 27, 385, 460
Eco24I GRGCYC 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 182, 434
Eco53kI GAGCTC 1 cut(s) 294
Eco57I CTGAAG 2 cut(s) 198, 603
Eco88I CYCGRG 2 cut(s) 336, 413
EcoICRI GAGCTC 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 182
EcoT14I CCWWGG 3 cut(s) 27, 385, 460
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 3 cut(s) 27, 385, 460
FaeI CATG 5 cut(s) 70, 395, 464, 526, 553
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FaqI GGGAC 1 cut(s) 669
FatI CATG 5 cut(s) 66, 391, 460, 522, 549
Fnu4HI GCNGC 6 cut(s) 15, 92, 261, 302, 402, 513
FokI GGATG 2 cut(s) 155, 572
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 6 cut(s) 15, 92, 261, 302, 402, 513
FspBI CTAG 1 cut(s) 555
GluI GCNGC 6 cut(s) 15, 92, 261, 302, 402, 513
HaeIII GGCC 1 cut(s) 521
Hin1II CATG 5 cut(s) 70, 395, 464, 526, 553
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 2 cut(s) 332, 530
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 1 cut(s) 729
Hpy188III TCNNGA 3 cut(s) 329, 364, 597
Hpy8I GTNNAC 1 cut(s) 729
HpyAV CCTTC 6 cut(s) 173, 539, 557, 710, 772, 788
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4V TGCA 5 cut(s) 74, 304, 310, 445, 806
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 307, 518
HpyF3I CTNAG 3 cut(s) 6, 651, 774
Hsp92II CATG 5 cut(s) 70, 395, 464, 526, 553
Kzo9I GATC 4 cut(s) 46, 213, 464, 759
LmnI GCTCC 2 cut(s) 158, 248
LpnPI CCDG 5 cut(s) 237, 309, 417, 486, 582
Lsp1109I GCAGC 4 cut(s) 103, 247, 288, 388
LweI GCATC 2 cut(s) 319, 793
MaeI CTAG 1 cut(s) 555
MaeIII GTNAC 4 cut(s) 277, 343, 427, 577
MalI GATC 4 cut(s) 48, 215, 466, 761
MboI GATC 4 cut(s) 46, 213, 464, 759
MboII GAAGA 4 cut(s) 69, 118, 742, 748
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 2 cut(s) 296, 414
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 2 cut(s) 379, 600
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 1 cut(s) 539
MnlI CCTC 5 cut(s) 265, 666, 705, 736, 791
Mox20I TGGCCA 1 cut(s) 521
MscI TGGCCA 1 cut(s) 521
MseI TTAA 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 521
MspA1I CMGCKG 2 cut(s) 301, 401
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 3 cut(s) 257, 307, 518
NcoI CCATGG 1 cut(s) 460
NdeII GATC 4 cut(s) 46, 213, 464, 759
NlaIII CATG 5 cut(s) 70, 395, 464, 526, 553
NlaIV GGNNCC 6 cut(s) 115, 184, 228, 436, 677, 687
NmuCI GTSAC 2 cut(s) 343, 427
NspI RCATGY 1 cut(s) 70
PfeI GAWTC 1 cut(s) 332
PflMI CCANNNNNTGG 1 cut(s) 223
PkrI GCNGC 6 cut(s) 16, 93, 262, 303, 403, 514
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
PpuMI RGGWCCY 1 cut(s) 182
Psp124BI GAGCTC 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 182
PspN4I GGNNCC 6 cut(s) 115, 184, 228, 436, 677, 687
PspPI GGNCC 2 cut(s) 182, 434
PspPPI RGGWCCY 1 cut(s) 182
PstNI CAGNNNCTG 1 cut(s) 275
PsuI RGATCY 1 cut(s) 213
PvuII CAGCTG 2 cut(s) 301, 401
RsaI GTAC 2 cut(s) 539, 700
RsaNI GTAC 2 cut(s) 538, 699
SacI GAGCTC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 6 cut(s) 15, 92, 261, 302, 402, 513
Sau3AI GATC 4 cut(s) 46, 213, 464, 759
Sau96I GGNCC 2 cut(s) 182, 434
SchI GAGTC 1 cut(s) 539
SduI GDGCHC 2 cut(s) 296, 414
SfaNI GCATC 2 cut(s) 319, 793
SinI GGWCC 2 cut(s) 182, 434
SmlI CTYRAG 2 cut(s) 362, 670
SmoI CTYRAG 2 cut(s) 362, 670
Sse9I AATT 2 cut(s) 379, 600
SsiI CCGC 2 cut(s) 14, 513
SspMI CTAG 1 cut(s) 555
SstI GAGCTC 1 cut(s) 296
StyI CCWWGG 3 cut(s) 27, 385, 460
TaaI ACNGT 1 cut(s) 206
TaqI TCGA 1 cut(s) 55
TaqII GACCGA 1 cut(s) 755
TasI AATT 2 cut(s) 379, 600
TatI WGTACW 1 cut(s) 537
TauI GCSGC 2 cut(s) 17, 515
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 3 cut(s) 436, 483, 715
TseFI GTSAC 2 cut(s) 343, 427
TseI GCWGC 4 cut(s) 91, 260, 301, 401
Tsp45I GTSAC 2 cut(s) 343, 427
TspDTI ATGAA 1 cut(s) 576
TspRI CASTG 3 cut(s) 436, 483, 715
Van91I CCANNNNNTGG 1 cut(s) 223
VpaK11BI GGWCC 2 cut(s) 182, 434
XapI RAATTY 1 cut(s) 600
XceI RCATGY 1 cut(s) 70
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.