RLG00000022199

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002814
Physical Location & Seq
Reverse (-)
81670306 .. 81672141
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022199

Sequence Viewer

Length: 807 bp
ATGGATGTTCCTCCTAAGGGTGGGTTTAGCTTTGATCTCTGTAAGCGAAATGCGATGCTGGCGAAGGATGGGGTTCAGCTACCTCCTTTTAGAAAGACCGGAACCACCATTGTGGGTTTGATTTATCAGGATGGCGTGGTCCTCGGAGCAGATACAAGGGCCACTGAAGGACCCATTGTAGCTGATAAGAACTGCGAGAAGATCCATTACTTGGCGCCCAATATTTATTGCTGTGGTGCGGGAACTGCTGCTGACACTGAGGCAGTTACAGATATGGTAAGCGCACAGCTGCAGTTGCACCGTTACCATACCGGCAGGGAGTCAAGGGTTGTGACTGCCCTCACCTATCTCAAAAGACATCTTTTCAGTTACCAAGGTCATGTCTCGGCTGCATTGGTGCTGGGTGGTGTTGATTTCACTGGCCCTCATTTGCACACTATATATCCTCATGGATCTACTGACACACTTCCTTATGCTACTATGGGCTCTGGCTCACTGGCTGCGATGGCAATATTTGAGTCCAAGTACAAGGAGGGGTTAACAAGAGATGAAGGTGTACAATTGGTGACAGAAGCTATTTGTGCGGGAATCTTCAATGACCTTGGAAGTGGTAGCAATGTTGACATTTGTGTTATAACCAAGGGAAGCAAAGAATATCTCCGAAACCACCTTCAGCCTAATCCCCGCACATATGTTACTCCCAAGGAATATACTTTCAGCAAGAAGACTGAAGTACTCTCCACCCTTGTTACTACACTGAAGGAGAGAGTAGAAGTGGTGGAAGCTGGAGATAAAATGGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

269

Amino Acids

29.11

Weight (kDa)

6.31

Isoelectric Point (pI)

25.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 32 - 212 5.2e-50 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
AccB1I GGYRCC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 211
AciI CCGC 3 cut(s) 239, 584, 685
AclWI GGATC 2 cut(s) 196, 460
AcuI CTGAAG 4 cut(s) 186, 656, 750, 779
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 3 cut(s) 527, 558, 735
AfiI CCNNNNNNNGG 3 cut(s) 17, 20, 211
AgsI TTSAA 1 cut(s) 595
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 6 cut(s) 30, 79, 182, 289, 575, 785
AluI AGCT 6 cut(s) 30, 79, 182, 289, 575, 785
Alw26I GTCTC 1 cut(s) 388
AlwI GGATC 2 cut(s) 196, 460
AoxI GGCC 2 cut(s) 159, 421
ApeKI GCWGC 4 cut(s) 248, 289, 389, 500
AspLEI GCGC 2 cut(s) 217, 284
AspS9I GGNCC 4 cut(s) 139, 159, 170, 422
AsuHPI GGTGA 2 cut(s) 334, 577
AvaII GGWCC 2 cut(s) 139, 170
AxyI CCTNAGG 1 cut(s) 15
BanI GGYRCC 1 cut(s) 214
BanII GRGCYC 1 cut(s) 488
BarI GAAGNNNNNNTAC 2 cut(s) 191, 223
BbsI GAAGAC 1 cut(s) 731
BbvI GCAGC 4 cut(s) 235, 276, 376, 487
BccI CCATC 3 cut(s) 62, 125, 499
BcoDI GTCTC 1 cut(s) 388
BfmI CTRYAG 1 cut(s) 290
BfoI RGCGCY 1 cut(s) 218
BisI GCNGC 4 cut(s) 249, 290, 390, 501
BlsI GCNGC 4 cut(s) 250, 291, 391, 502
BmcAI AGTACT 1 cut(s) 735
Bme18I GGWCC 2 cut(s) 139, 170
BmgT120I GGNCC 4 cut(s) 139, 159, 170, 422
BmiI GGNNCC 3 cut(s) 103, 172, 216
BmsI GCATC 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 731
BpmI CTGGAG 1 cut(s) 807
BsaHI GRCGYC 1 cut(s) 215
BsaJI CCNNGG 5 cut(s) 142, 373, 601, 639, 702
BsaWI WCCGGW 1 cut(s) 98
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 20, 211
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 2 cut(s) 424, 501
Bse21I CCTNAGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 622
BseDI CCNNGG 5 cut(s) 142, 373, 601, 639, 702
BseGI GGATG 3 cut(s) 10, 73, 136
BseLI CCNNNNNNNGG 3 cut(s) 17, 20, 211
BseMI GCAATG 1 cut(s) 622
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 2 cut(s) 424, 501
BseXI GCAGC 4 cut(s) 235, 276, 376, 487
BseYI CCCAGC 1 cut(s) 400
BshFI GGCC 2 cut(s) 161, 423
BshNI GGYRCC 1 cut(s) 214
BsiSI CCGG 2 cut(s) 99, 312
BslI CCNNNNNNNGG 3 cut(s) 17, 20, 211
BsmAI GTCTC 1 cut(s) 388
BsnI GGCC 2 cut(s) 161, 423
Bsp1286I GDGCHC 1 cut(s) 488
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 3 cut(s) 34, 201, 452
BspACI CCGC 3 cut(s) 239, 584, 685
BspANI GGCC 2 cut(s) 161, 423
BspCNI CTCAG 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 103, 172, 216
BspMAI CTGCAG 1 cut(s) 294
BspPI GGATC 2 cut(s) 196, 460
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 622
BsrFI RCCGGY 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 2 cut(s) 424, 501
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 5 cut(s) 142, 373, 601, 639, 702
BssMI GATC 3 cut(s) 34, 201, 452
BssNI GRCGYC 1 cut(s) 215
BssT1I CCWWGG 4 cut(s) 373, 601, 639, 702
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 1 cut(s) 795
BstACI GRCGYC 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 15, 258
BstF5I GGATG 3 cut(s) 10, 73, 136
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 2 cut(s) 217, 284
BstKTI GATC 3 cut(s) 37, 204, 455
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 3 cut(s) 34, 201, 452
BstMWI GCNNNNNNNGC 5 cut(s) 59, 245, 295, 506, 581
BstSFI CTRYAG 1 cut(s) 290
BstV1I GCAGC 4 cut(s) 235, 276, 376, 487
BstV2I GAAGAC 1 cut(s) 731
BstX2I RGATCY 2 cut(s) 201, 452
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 2 cut(s) 201, 452
Bsu36I CCTNAGG 1 cut(s) 15
BsuRI GGCC 2 cut(s) 161, 423
BtgZI GCGATG 2 cut(s) 68, 518
BtsCI GGATG 3 cut(s) 10, 73, 136
BtsIMutI CAGTG 5 cut(s) 162, 255, 417, 494, 755
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 2 cut(s) 217, 284
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 4 cut(s) 139, 159, 170, 422
Csp6I GTAC 3 cut(s) 526, 557, 734
CviAII CATG 2 cut(s) 380, 449
CviQI GTAC 3 cut(s) 526, 557, 734
DdeI CTNAG 2 cut(s) 15, 258
DinI GGCGCC 1 cut(s) 216
DpnI GATC 3 cut(s) 36, 203, 454
DpnII GATC 3 cut(s) 34, 201, 452
Eam1104I CTCTTC 1 cut(s) 795
EarI CTCTTC 1 cut(s) 795
Eco130I CCWWGG 4 cut(s) 373, 601, 639, 702
Eco24I GRGCYC 1 cut(s) 488
Eco47I GGWCC 2 cut(s) 139, 170
Eco57I CTGAAG 4 cut(s) 186, 656, 750, 779
Eco81I CCTNAGG 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 170
EcoT14I CCWWGG 4 cut(s) 373, 601, 639, 702
EcoT38I GRGCYC 1 cut(s) 488
EgeI GGCGCC 1 cut(s) 216
EheI GGCGCC 1 cut(s) 216
ErhI CCWWGG 4 cut(s) 373, 601, 639, 702
FaeI CATG 2 cut(s) 383, 452
FatI CATG 2 cut(s) 379, 448
FauI CCCGC 3 cut(s) 232, 577, 692
FauNDI CATATG 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 249, 290, 390, 501
FokI GGATG 3 cut(s) 17, 80, 143
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 4 cut(s) 249, 290, 390, 501
GlaI GCGC 2 cut(s) 216, 283
GluI GCNGC 4 cut(s) 249, 290, 390, 501
GsaI CCCAGC 1 cut(s) 404
GsuI CTGGAG 1 cut(s) 807
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 2 cut(s) 161, 423
HapII CCGG 2 cut(s) 99, 312
HhaI GCGC 2 cut(s) 217, 284
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 2 cut(s) 383, 452
Hin6I GCGC 2 cut(s) 215, 282
HinP1I GCGC 2 cut(s) 215, 282
HincII GTYRAC 2 cut(s) 540, 622
HindII GTYRAC 2 cut(s) 540, 622
HinfI GANTC 3 cut(s) 320, 518, 588
HpaI GTTAAC 1 cut(s) 540
HpaII CCGG 2 cut(s) 99, 312
HphI GGTGA 2 cut(s) 334, 577
Hpy166II GTNNAC 3 cut(s) 540, 557, 622
Hpy188I TCNGA 2 cut(s) 146, 662
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 3 cut(s) 540, 557, 622
HpyAV CCTTC 5 cut(s) 58, 161, 545, 680, 754
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4V TGCA 4 cut(s) 292, 298, 392, 433
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 245, 295, 506, 581
HpyF3I CTNAG 2 cut(s) 15, 258
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 2 cut(s) 383, 452
HspAI GCGC 2 cut(s) 215, 282
KasI GGCGCC 1 cut(s) 214
KspAI GTTAAC 1 cut(s) 540
Kzo9I GATC 3 cut(s) 34, 201, 452
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 4 cut(s) 235, 276, 376, 487
LweI GCATC 1 cut(s) 45
MaeIII GTNAC 7 cut(s) 265, 302, 331, 368, 565, 694, 748
MalI GATC 3 cut(s) 36, 203, 454
MboI GATC 3 cut(s) 34, 201, 452
MboII GAAGA 3 cut(s) 211, 583, 736
MfeI CAATTG 1 cut(s) 560
MflI RGATCY 2 cut(s) 201, 452
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 1 cut(s) 560
Mly113I GGCGCC 1 cut(s) 215
MlyI GAGTC 2 cut(s) 329, 527
MnlI CCTC 8 cut(s) 21, 93, 152, 253, 350, 435, 456, 526
MseI TTAA 1 cut(s) 539
MslI CAYNNNNRTG 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 2 cut(s) 99, 312
MunI CAATTG 1 cut(s) 560
MwoI GCNNNNNNNGC 5 cut(s) 59, 245, 295, 506, 581
NarI GGCGCC 1 cut(s) 215
NdeI CATATG 1 cut(s) 691
NdeII GATC 3 cut(s) 34, 201, 452
NlaIII CATG 2 cut(s) 383, 452
NlaIV GGNNCC 3 cut(s) 103, 172, 216
NmeAIII GCCGAG 1 cut(s) 365
NmuCI GTSAC 2 cut(s) 331, 565
OliI CACNNNNGTG 1 cut(s) 110
PfeI GAWTC 1 cut(s) 588
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 4 cut(s) 250, 291, 391, 502
PleI GAGTC 2 cut(s) 328, 526
PluTI GGCGCC 1 cut(s) 218
PpsI GAGTC 2 cut(s) 328, 526
PpuMI RGGWCCY 1 cut(s) 170
PsiI TTATAA 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 400
PspN4I GGNNCC 3 cut(s) 103, 172, 216
PspPI GGNCC 4 cut(s) 139, 159, 170, 422
PspPPI RGGWCCY 1 cut(s) 170
PstI CTGCAG 1 cut(s) 294
PsuI RGATCY 2 cut(s) 201, 452
PvuII CAGCTG 1 cut(s) 289
RsaI GTAC 3 cut(s) 527, 558, 735
RsaNI GTAC 3 cut(s) 526, 557, 734
RseI CAYNNNNRTG 1 cut(s) 110
SaqAI TTAA 1 cut(s) 539
SatI GCNGC 4 cut(s) 249, 290, 390, 501
Sau3AI GATC 3 cut(s) 34, 201, 452
Sau96I GGNCC 4 cut(s) 139, 159, 170, 422
ScaI AGTACT 1 cut(s) 735
SchI GAGTC 2 cut(s) 329, 527
SduI GDGCHC 1 cut(s) 488
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 1 cut(s) 290
SfoI GGCGCC 1 cut(s) 216
SinI GGWCC 2 cut(s) 139, 170
SmiMI CAYNNNNRTG 1 cut(s) 110
Sse9I AATT 1 cut(s) 560
SsiI CCGC 3 cut(s) 239, 584, 685
SspDI GGCGCC 1 cut(s) 214
SspI AATATT 2 cut(s) 223, 513
StyI CCWWGG 4 cut(s) 373, 601, 639, 702
TaaI ACNGT 1 cut(s) 302
TasI AATT 1 cut(s) 560
TatI WGTACW 3 cut(s) 525, 556, 733
TfiI GAWTC 1 cut(s) 588
Tru1I TTAA 1 cut(s) 539
Tru9I TTAA 1 cut(s) 539
TscAI CASTG 5 cut(s) 169, 262, 424, 501, 762
TseFI GTSAC 2 cut(s) 331, 565
TseI GCWGC 4 cut(s) 248, 289, 389, 500
Tsp45I GTSAC 2 cut(s) 331, 565
TspDTI ATGAA 1 cut(s) 564
TspRI CASTG 5 cut(s) 169, 262, 424, 501, 762
Van91I CCANNNNNTGG 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 139, 170
ZrmI AGTACT 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.