Rw3G013680

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002814
Physical Location & Seq
Reverse (-)
13525482 .. 13527338
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013680.1

Sequence Viewer

Length: 822 bp
ATGTCTAAGTTAGCCATGGATGTTCCTCCCAAGGGTGGGTTTAGCTTTGATCTCTGTAAGCGAAATGCGATGCTGGCGAAGGACGGGGTTCAGCTACCTCCTTTTAGAAAGACCGGAACCACCATTGTGGGTTTGATTTATCAGGATGGTGTAGTCCTGGGAGCAGATACAAGGGCCACTGAAGGACCCATTGTAGCTGATAAGAACTGCGAGAAGATCCATTACTTGGCACCCAATATTTATTGCTGTGGTGCGGGAACTGCTGCTGACACTGAGGCAGTTACAGATATGGTAAGCGCACAGCTGCAGTTGCACCGTTACCATACCGGCAGGGAGTCAAGGGTTGTGACTGCCCTCACCTATCTCAAAAGACATCTTTTCAGTTACCAAGGTCATGTCTCAGCTGCATTGGTGCTGGGTGGTGTTGATTTCACTGGCCCTCATTTGCACACTATATATCCTCATGGATCTACTGACACACTTCCTTATGCTACTATGGGCTCTGGCTCACTGGCTGCGATGGCAATATTTGAGTCCAAGTACAAGGAGGGGTTAACAAGAGATGAAGGTGTACAATTGGTGACAGAAGCTATTTGTGCGGGAATCTTCAATGACCTTGGAAGTGGTAGCAATGTTGACATTTGTGTTATAACCAAGGGAAGCAAAGAATATCTCCGAAACCACCTTCAGCCTAATCCCCGCACATATGTTACTCCCAAGGAATATACTTTCAGCAAGAAGACTGAAGTACTCTCCACCCTTGTTACTACACTGAAGGAGAGAGTGGAAGTGGTGGAAGCTGGAGATAAAATGGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

273

Amino Acids

29.64

Weight (kDa)

6.51

Isoelectric Point (pI)

26.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 37 - 217 5.5e-50 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 229
AccB7I CCANNNNNTGG 1 cut(s) 226
AciI CCGC 3 cut(s) 254, 599, 700
AclWI GGATC 2 cut(s) 211, 475
AcuI CTGAAG 4 cut(s) 201, 671, 765, 794
AfaI GTAC 3 cut(s) 542, 573, 750
AfiI CCNNNNNNNGG 4 cut(s) 32, 35, 36, 226
AgsI TTSAA 1 cut(s) 610
AjnI CCWGG 1 cut(s) 156
AleI CACNNNNGTG 1 cut(s) 125
AluBI AGCT 7 cut(s) 45, 94, 197, 304, 404, 590, 800
AluI AGCT 7 cut(s) 45, 94, 197, 304, 404, 590, 800
Alw26I GTCTC 1 cut(s) 403
AlwI GGATC 2 cut(s) 211, 475
AoxI GGCC 2 cut(s) 174, 436
ApeKI GCWGC 4 cut(s) 263, 304, 404, 515
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 3 cut(s) 174, 185, 437
AsuHPI GGTGA 2 cut(s) 349, 592
AvaII GGWCC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 503
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 1 cut(s) 746
BbvI GCAGC 4 cut(s) 250, 291, 391, 502
BccI CCATC 2 cut(s) 140, 514
BciT130I CCWGG 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 403
BfmI CTRYAG 1 cut(s) 305
BisI GCNGC 4 cut(s) 264, 305, 405, 516
BlsI GCNGC 4 cut(s) 265, 306, 406, 517
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 3 cut(s) 174, 185, 437
BmiI GGNNCC 3 cut(s) 118, 187, 231
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 1 cut(s) 60
BpiI GAAGAC 1 cut(s) 746
BpmI CTGGAG 1 cut(s) 822
BsaJI CCNNGG 7 cut(s) 15, 30, 157, 388, 616, 654, 717
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 35, 36, 226
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 439, 516
Bse3DI GCAATG 1 cut(s) 637
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 7 cut(s) 15, 30, 157, 388, 616, 654, 717
BseGI GGATG 2 cut(s) 25, 151
BseLI CCNNNNNNNGG 4 cut(s) 32, 35, 36, 226
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 2 cut(s) 264, 414
BseNI ACTGG 2 cut(s) 439, 516
BseXI GCAGC 4 cut(s) 250, 291, 391, 502
BseYI CCCAGC 1 cut(s) 415
BshFI GGCC 2 cut(s) 176, 438
BshNI GGYRCC 1 cut(s) 229
BsiSI CCGG 2 cut(s) 114, 327
BslI CCNNNNNNNGG 4 cut(s) 32, 35, 36, 226
BsmAI GTCTC 1 cut(s) 403
BsnI GGCC 2 cut(s) 176, 438
Bsp1286I GDGCHC 1 cut(s) 503
Bsp1407I TGTACA 1 cut(s) 571
Bsp143I GATC 3 cut(s) 49, 216, 467
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 3 cut(s) 254, 599, 700
BspANI GGCC 2 cut(s) 176, 438
BspCNI CTCAG 2 cut(s) 265, 413
BspLI GGNNCC 3 cut(s) 118, 187, 231
BspMAI CTGCAG 1 cut(s) 309
BspPI GGATC 2 cut(s) 211, 475
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 637
BsrFI RCCGGY 1 cut(s) 326
BsrGI TGTACA 1 cut(s) 571
BsrI ACTGG 2 cut(s) 439, 516
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 7 cut(s) 15, 30, 157, 388, 616, 654, 717
BssMI GATC 3 cut(s) 49, 216, 467
BssT1I CCWWGG 6 cut(s) 15, 30, 388, 616, 654, 717
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 810
BstAUI TGTACA 1 cut(s) 571
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 3 cut(s) 6, 273, 400
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 25, 151
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 3 cut(s) 52, 219, 470
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 3 cut(s) 49, 216, 467
BstMWI GCNNNNNNNGC 5 cut(s) 74, 260, 310, 521, 596
BstNI CCWGG 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 156
BstSFI CTRYAG 1 cut(s) 305
BstV1I GCAGC 4 cut(s) 250, 291, 391, 502
BstV2I GAAGAC 1 cut(s) 746
BstX2I RGATCY 2 cut(s) 216, 467
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 2 cut(s) 216, 467
BsuRI GGCC 2 cut(s) 176, 438
BtgI CCRYGG 1 cut(s) 15
BtgZI GCGATG 2 cut(s) 83, 533
BtsCI GGATG 2 cut(s) 25, 151
BtsIMutI CAGTG 5 cut(s) 177, 270, 432, 509, 770
Cac8I GCNNGC 1 cut(s) 75
CfoI GCGC 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 3 cut(s) 174, 185, 437
Csp6I GTAC 3 cut(s) 541, 572, 749
CviAII CATG 3 cut(s) 16, 395, 464
CviQI GTAC 3 cut(s) 541, 572, 749
DdeI CTNAG 3 cut(s) 6, 273, 400
DpnI GATC 3 cut(s) 51, 218, 469
DpnII GATC 3 cut(s) 49, 216, 467
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
Eco130I CCWWGG 6 cut(s) 15, 30, 388, 616, 654, 717
Eco24I GRGCYC 1 cut(s) 503
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 4 cut(s) 201, 671, 765, 794
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 156
EcoT14I CCWWGG 6 cut(s) 15, 30, 388, 616, 654, 717
EcoT38I GRGCYC 1 cut(s) 503
ErhI CCWWGG 6 cut(s) 15, 30, 388, 616, 654, 717
FaeI CATG 3 cut(s) 19, 398, 467
FatI CATG 3 cut(s) 15, 394, 463
FauI CCCGC 3 cut(s) 247, 592, 707
FauNDI CATATG 1 cut(s) 706
Fnu4HI GCNGC 4 cut(s) 264, 305, 405, 516
FokI GGATG 2 cut(s) 32, 158
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 4 cut(s) 264, 305, 405, 516
GlaI GCGC 1 cut(s) 298
GluI GCNGC 4 cut(s) 264, 305, 405, 516
GsaI CCCAGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 822
HaeIII GGCC 2 cut(s) 176, 438
HapII CCGG 2 cut(s) 114, 327
HhaI GCGC 1 cut(s) 299
Hin1II CATG 3 cut(s) 19, 398, 467
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HincII GTYRAC 2 cut(s) 555, 637
HindII GTYRAC 2 cut(s) 555, 637
HinfI GANTC 3 cut(s) 335, 533, 603
HpaI GTTAAC 1 cut(s) 555
HpaII CCGG 2 cut(s) 114, 327
HphI GGTGA 2 cut(s) 349, 592
Hpy166II GTNNAC 3 cut(s) 555, 572, 637
Hpy188I TCNGA 1 cut(s) 677
Hpy188III TCNNGA 1 cut(s) 143
Hpy8I GTNNAC 3 cut(s) 555, 572, 637
HpyAV CCTTC 5 cut(s) 73, 176, 560, 695, 769
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4V TGCA 4 cut(s) 307, 313, 407, 448
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 260, 310, 521, 596
HpyF3I CTNAG 3 cut(s) 6, 273, 400
Hsp92II CATG 3 cut(s) 19, 398, 467
HspAI GCGC 1 cut(s) 297
KspAI GTTAAC 1 cut(s) 555
Kzo9I GATC 3 cut(s) 49, 216, 467
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 4 cut(s) 250, 291, 391, 502
LweI GCATC 1 cut(s) 60
MaeIII GTNAC 7 cut(s) 280, 317, 346, 383, 580, 709, 763
MalI GATC 3 cut(s) 51, 218, 469
MboI GATC 3 cut(s) 49, 216, 467
MboII GAAGA 3 cut(s) 226, 598, 751
MfeI CAATTG 1 cut(s) 575
MflI RGATCY 2 cut(s) 216, 467
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 1 cut(s) 575
MlyI GAGTC 2 cut(s) 344, 542
MnlI CCTC 7 cut(s) 36, 108, 268, 365, 450, 471, 541
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 1 cut(s) 125
MspA1I CMGCKG 2 cut(s) 304, 404
MspI CCGG 2 cut(s) 114, 327
MspR9I CCNGG 1 cut(s) 158
MunI CAATTG 1 cut(s) 575
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 5 cut(s) 74, 260, 310, 521, 596
NcoI CCATGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 706
NdeII GATC 3 cut(s) 49, 216, 467
NlaIII CATG 3 cut(s) 19, 398, 467
NlaIV GGNNCC 3 cut(s) 118, 187, 231
NmuCI GTSAC 2 cut(s) 346, 580
OliI CACNNNNGTG 1 cut(s) 125
PfeI GAWTC 1 cut(s) 603
PflMI CCANNNNNTGG 1 cut(s) 226
PkrI GCNGC 4 cut(s) 265, 306, 406, 517
PleI GAGTC 2 cut(s) 343, 541
PpsI GAGTC 2 cut(s) 343, 541
PpuMI RGGWCCY 1 cut(s) 185
PsiI TTATAA 1 cut(s) 650
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 156
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 3 cut(s) 118, 187, 231
PspPI GGNCC 3 cut(s) 174, 185, 437
PspPPI RGGWCCY 1 cut(s) 185
PstI CTGCAG 1 cut(s) 309
PsuI RGATCY 2 cut(s) 216, 467
PvuII CAGCTG 2 cut(s) 304, 404
RsaI GTAC 3 cut(s) 542, 573, 750
RsaNI GTAC 3 cut(s) 541, 572, 749
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 4 cut(s) 264, 305, 405, 516
Sau3AI GATC 3 cut(s) 49, 216, 467
Sau96I GGNCC 3 cut(s) 174, 185, 437
ScaI AGTACT 1 cut(s) 750
SchI GAGTC 2 cut(s) 344, 542
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 1 cut(s) 503
SfaNI GCATC 1 cut(s) 60
SfcI CTRYAG 1 cut(s) 305
SinI GGWCC 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 1 cut(s) 575
SsiI CCGC 3 cut(s) 254, 599, 700
SspI AATATT 2 cut(s) 238, 528
StyD4I CCNGG 1 cut(s) 156
StyI CCWWGG 6 cut(s) 15, 30, 388, 616, 654, 717
TaaI ACNGT 1 cut(s) 317
TasI AATT 1 cut(s) 575
TatI WGTACW 3 cut(s) 540, 571, 748
TfiI GAWTC 1 cut(s) 603
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 5 cut(s) 184, 277, 439, 516, 777
TseFI GTSAC 2 cut(s) 346, 580
TseI GCWGC 4 cut(s) 263, 304, 404, 515
Tsp45I GTSAC 2 cut(s) 346, 580
TspDTI ATGAA 1 cut(s) 579
TspRI CASTG 5 cut(s) 184, 277, 439, 516, 777
Van91I CCANNNNNTGG 1 cut(s) 226
VpaK11BI GGWCC 1 cut(s) 185
ZrmI AGTACT 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.