AT5G40580

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002814
Physical Location & Seq
Reverse (-)
16248286 .. 16250925
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G40580.1

Sequence Viewer

Length: 825 bp
ATGTCGCAGTCTTCCGTCGATATACCGCCAAAAGGTGGGTTTAGCTTCGATCTGTGCAAAAGGAATGATATGCTGACACAGAAGGGTCTTAAAGCGCCGTCGTTTCTCAAGACTGGAACGACTATCGTTGGATTGATATTTAAGGATGGTGTAATACTTGGGGCTGATACACGGGCAACTGAAGGGCCTATTGTGGCTGATAAGAACTGTGAGAAGATTCACTACATGGCACCAAACATTTACTGTTGTGGTGCAGGAACCGCTGCTGACACTGAAGCAGTAACTGATATGGTCAGCTCACAGCTGCGGTTGCATAGATACCAGACTGGCCGAGATTCTCGAGTCGTTACTGCATTGACCCTTCTCAAAAAACATCTTTTCAGCTACCAAGGCCATGTCTCAGCTGCTCTTGTGCTTGGTGGAGTTGATATTACCGGGCCTCATCTTCACACGATATACCCACACGGTTCTACTGACACTCTACCATTTGCTACAATGGGTTCTGGTTCCCTTGCTGCCATGTCAGTGTTTGAAGCAAAGTATAAAGAAGGTCTAACTAGAGATGAAGGAATCAAGTTGGTTGCTGAAGCCATATGCTCTGGTATATTCAACGACCTTGGTAGTGGTAGCAATGTGGATATCTGTGTGATCACTAAGGGGCACAAGGAATACTTGAGGAATTACATGGAACCAAACCCAAGAACCTATGTTAGCAGCAAAGGCTATTCATTCACCAAGAAAACCGAGGTTCTACTCACCAAAATTACACCATTATTGGAGCGAGTCGAAATTGTAGAAGTGGCTGGTGAAGCTATGGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

274

Amino Acids

29.62

Weight (kDa)

6.71

Isoelectric Point (pI)

27.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 37 - 217 2.1e-50 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 229
AccB7I CCANNNNNTGG 1 cut(s) 35
AciI CCGC 3 cut(s) 26, 261, 307
AcoI YGGCCR 1 cut(s) 328
AcuI CTGAAG 3 cut(s) 201, 294, 606
AfiI CCNNNNNNNGG 2 cut(s) 32, 35
AgsI TTSAA 2 cut(s) 533, 610
AluBI AGCT 6 cut(s) 45, 297, 304, 384, 404, 812
AluI AGCT 6 cut(s) 45, 297, 304, 384, 404, 812
Alw26I GTCTC 1 cut(s) 403
AlwNI CAGNNNCTG 1 cut(s) 284
Ama87I CYCGRG 1 cut(s) 339
AoxI GGCC 4 cut(s) 185, 328, 391, 437
ApeKI GCWGC 5 cut(s) 263, 304, 404, 515, 714
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 2 cut(s) 185, 437
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 3 cut(s) 724, 748, 818
AvaI CYCGRG 1 cut(s) 339
BaeGI GKGCMC 1 cut(s) 663
BanI GGYRCC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 1 cut(s) 3
BbvI GCAGC 5 cut(s) 250, 291, 391, 502, 726
BccI CCATC 1 cut(s) 140
BceAI ACGGC 1 cut(s) 82
BclI TGATCA 1 cut(s) 648
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 1 cut(s) 403
BfaI CTAG 1 cut(s) 558
BfoI RGCGCY 1 cut(s) 98
BisI GCNGC 5 cut(s) 264, 305, 405, 516, 715
BlsI GCNGC 5 cut(s) 265, 306, 406, 517, 716
Bme1390I CCNGG 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 2 cut(s) 185, 437
BmiI GGNNCC 4 cut(s) 231, 259, 508, 690
BmrFI CCNGG 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 3
BpuEI CTTGAG 2 cut(s) 92, 694
BpuMI CCSGG 1 cut(s) 436
BsaJI CCNNGG 3 cut(s) 388, 616, 744
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 35
Bse1I ACTGG 2 cut(s) 118, 331
Bse3DI GCAATG 1 cut(s) 637
BseDI CCNNGG 3 cut(s) 388, 616, 744
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 2 cut(s) 32, 35
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 2 cut(s) 118, 331
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 5 cut(s) 250, 291, 391, 502, 726
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 4 cut(s) 187, 330, 393, 439
BshNI GGYRCC 1 cut(s) 229
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 1 cut(s) 435
BslI CCNNNNNNNGG 2 cut(s) 32, 35
BsmAI GTCTC 1 cut(s) 403
BsnI GGCC 4 cut(s) 187, 330, 393, 439
BsoBI CYCGRG 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 663
Bsp143I GATC 2 cut(s) 49, 648
BspACI CCGC 3 cut(s) 26, 261, 307
BspANI GGCC 4 cut(s) 187, 330, 393, 439
BspCNI CTCAG 1 cut(s) 413
BspLI GGNNCC 4 cut(s) 231, 259, 508, 690
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 637
BsrI ACTGG 2 cut(s) 118, 331
BssECI CCNNGG 3 cut(s) 388, 616, 744
BssMI GATC 2 cut(s) 49, 648
BssT1I CCWWGG 2 cut(s) 388, 616
Bst4CI ACNGT 3 cut(s) 209, 245, 467
BstDEI CTNAG 2 cut(s) 400, 654
BstF5I GGATG 1 cut(s) 151
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 2 cut(s) 52, 651
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 2 cut(s) 49, 648
BstMWI GCNNNNNNNGC 5 cut(s) 260, 310, 390, 720, 809
BstSCI CCNGG 1 cut(s) 434
BstSLI GKGCMC 1 cut(s) 663
BstV1I GCAGC 5 cut(s) 250, 291, 391, 502, 726
BstV2I GAAGAC 1 cut(s) 3
BsuRI GGCC 4 cut(s) 187, 330, 393, 439
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 2 cut(s) 270, 531
CaiI CAGNNNCTG 1 cut(s) 284
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 2 cut(s) 185, 437
CviAII CATG 4 cut(s) 226, 395, 520, 685
DdeI CTNAG 2 cut(s) 400, 654
DpnI GATC 2 cut(s) 51, 650
DpnII GATC 2 cut(s) 49, 648
EaeI YGGCCR 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 388, 616
Eco32I GATATC 1 cut(s) 640
Eco57I CTGAAG 3 cut(s) 201, 294, 606
Eco88I CYCGRG 1 cut(s) 339
EcoO109I RGGNCCY 1 cut(s) 185
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 388, 616
ErhI CCWWGG 2 cut(s) 388, 616
FaeI CATG 4 cut(s) 229, 398, 523, 688
FalI AAGNNNNNCTT 2 cut(s) 656, 688
FatI CATG 4 cut(s) 225, 394, 519, 684
FauNDI CATATG 1 cut(s) 593
FbaI TGATCA 1 cut(s) 648
Fnu4HI GCNGC 5 cut(s) 264, 305, 405, 516, 715
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 5 cut(s) 264, 305, 405, 516, 715
FspBI CTAG 1 cut(s) 558
GlaI GCGC 1 cut(s) 96
GluI GCNGC 5 cut(s) 264, 305, 405, 516, 715
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 4 cut(s) 187, 330, 393, 439
HapII CCGG 1 cut(s) 435
HhaI GCGC 1 cut(s) 97
Hin1II CATG 4 cut(s) 229, 398, 523, 688
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HinfI GANTC 5 cut(s) 217, 335, 342, 570, 783
HpaII CCGG 1 cut(s) 435
HphI GGTGA 3 cut(s) 724, 748, 818
Hpy188III TCNNGA 2 cut(s) 109, 339
Hpy99I CGWCG 2 cut(s) 20, 103
HpyAV CCTTC 5 cut(s) 76, 176, 371, 542, 560
HpyCH4III ACNGT 3 cut(s) 209, 245, 467
HpyCH4V TGCA 4 cut(s) 57, 254, 313, 353
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 310, 390, 720, 809
HpyF3I CTNAG 2 cut(s) 400, 654
Hsp92II CATG 4 cut(s) 229, 398, 523, 688
HspAI GCGC 1 cut(s) 95
Ksp22I TGATCA 1 cut(s) 648
Kzo9I GATC 2 cut(s) 49, 648
LmnI GCTCC 1 cut(s) 778
LpnPI CCDG 8 cut(s) 99, 240, 312, 335, 448, 489, 585, 789
Lsp1109I GCAGC 5 cut(s) 250, 291, 391, 502, 726
MaeI CTAG 1 cut(s) 558
MaeIII GTNAC 2 cut(s) 280, 346
MalI GATC 2 cut(s) 51, 650
MboI GATC 2 cut(s) 49, 648
MboII GAAGA 3 cut(s) 3, 226, 437
MhlI GDGCHC 1 cut(s) 663
MluCI AATT 3 cut(s) 679, 762, 789
MlyI GAGTC 2 cut(s) 351, 792
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 4 cut(s) 450, 669, 739, 811
MseI TTAA 2 cut(s) 90, 141
MslI CAYNNNNRTG 1 cut(s) 524
MspA1I CMGCKG 3 cut(s) 263, 304, 404
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 1 cut(s) 436
MwoI GCNNNNNNNGC 5 cut(s) 260, 310, 390, 720, 809
NciI CCSGG 1 cut(s) 436
NdeI CATATG 1 cut(s) 593
NdeII GATC 2 cut(s) 49, 648
NlaIII CATG 4 cut(s) 229, 398, 523, 688
NlaIV GGNNCC 4 cut(s) 231, 259, 508, 690
NmeAIII GCCGAG 1 cut(s) 356
PaeR7I CTCGAG 1 cut(s) 339
PfeI GAWTC 3 cut(s) 217, 335, 570
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 5 cut(s) 265, 306, 406, 517, 716
PleI GAGTC 2 cut(s) 350, 791
PpsI GAGTC 2 cut(s) 350, 791
PspN4I GGNNCC 4 cut(s) 231, 259, 508, 690
PspPI GGNCC 2 cut(s) 185, 437
PstNI CAGNNNCTG 1 cut(s) 284
PvuII CAGCTG 2 cut(s) 304, 404
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 2 cut(s) 90, 141
SatI GCNGC 5 cut(s) 264, 305, 405, 516, 715
Sau3AI GATC 2 cut(s) 49, 648
Sau96I GGNCC 2 cut(s) 185, 437
SchI GAGTC 2 cut(s) 351, 792
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 1 cut(s) 663
Sfr274I CTCGAG 1 cut(s) 339
SlaI CTCGAG 1 cut(s) 339
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 3 cut(s) 107, 339, 673
SmoI CTYRAG 3 cut(s) 107, 339, 673
Sse9I AATT 3 cut(s) 679, 762, 789
SsiI CCGC 3 cut(s) 26, 261, 307
SspMI CTAG 1 cut(s) 558
StyD4I CCNGG 1 cut(s) 434
StyI CCWWGG 2 cut(s) 388, 616
TaaI ACNGT 3 cut(s) 209, 245, 467
TaqI TCGA 4 cut(s) 18, 48, 340, 786
TasI AATT 3 cut(s) 679, 762, 789
TfiI GAWTC 3 cut(s) 217, 335, 570
Tru1I TTAA 2 cut(s) 90, 141
Tru9I TTAA 2 cut(s) 90, 141
TscAI CASTG 2 cut(s) 277, 531
TseI GCWGC 5 cut(s) 263, 304, 404, 515, 714
TspDTI ATGAA 2 cut(s) 579, 717
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 2 cut(s) 277, 531
Van91I CCANNNNNTGG 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 339
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.