Rw2G053320

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002814
Physical Location & Seq
Reverse (-)
81089763 .. 81092018
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053320.1

Sequence Viewer

Length: 756 bp
ATGGCTAAATCAATCATTGATGTTCCTCCCAAGGGTGGATTTAGCTTTGATCTATGTAAAAGAAATGCTATGCTTTCGAAGAATGCGGTTCAGCTACCTCCGTTTACCAAGACAGGAACCACCATTGTTGGGTTAATTTTTCAGGATGGTGTCATTCTTGGAGCTGATACCAGGGCCACCTCAGGGTCAATTGTGTGTGATAAAAACTGTGAGAAAATTCATTACATGGCACCAAACATATATTGCTGTGGGGCAGGAACTGCTGCTGATACTGAGGCAGTGACAGACATGGTCAGCTCACAGCTGCAATTGCACCGTTACCACACTGGTAGGGAATCAAGGGTTGTGACTGCCCTCACCTATCTCAAAAGACATCTTTTCAGTTACCAGGGTCATGTCTCGGCTGCATTGGTGCTTGGGGGTGTTGATTTCACCGGCCCTCATTTGCACACTATTTATCCACATGGGTCGACTGACACACTCCCTTATGCTACTATGGGTTCCGGCTCACTTGCTGCAATGGCCATCTTCGAGTCCAAGTACAAAGAAGGCCTGACTAGAGATGAAGGGGTACAGCTTGTTACAGAGGCTATTTGCTCTGGTATATTCAATGATTTGGGAAGTGGTAGCAATGTCGATATCTGTGTAATAACCAAGGGCAACAAAGAATTCCTGCGGAACCATTTGCAGCCTAATCCCGAACCTATGTCAGCCCCAAAGAATACTCATTCACCAAGAAGACCGAGGTTCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

251

Amino Acids

27.11

Weight (kDa)

8.39

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 37 - 217 1.1e-49 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 470
AciI CCGC 2 cut(s) 86, 676
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 2 cut(s) 216, 668
AfaI GTAC 2 cut(s) 542, 573
AfiI CCNNNNNNNGG 4 cut(s) 32, 35, 129, 183
AgsI TTSAA 1 cut(s) 610
AjnI CCWGG 2 cut(s) 170, 387
AluBI AGCT 6 cut(s) 45, 94, 164, 297, 304, 577
AluI AGCT 6 cut(s) 45, 94, 164, 297, 304, 577
Alw26I GTCTC 1 cut(s) 403
AlwNI CAGNNNCTG 1 cut(s) 260
AoxI GGCC 4 cut(s) 174, 436, 522, 550
ApeKI GCWGC 5 cut(s) 263, 304, 404, 515, 688
ApoI RAATTY 2 cut(s) 216, 668
AspS9I GGNCC 2 cut(s) 174, 437
AsuHPI GGTGA 3 cut(s) 349, 424, 723
AsuII TTCGAA 1 cut(s) 77
AxyI CCTNAGG 1 cut(s) 181
BalI TGGCCA 1 cut(s) 524
BanI GGYRCC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 745
BbvI GCAGC 5 cut(s) 250, 291, 391, 502, 700
BccI CCATC 2 cut(s) 140, 533
BciT130I CCWGG 2 cut(s) 172, 389
BcoDI GTCTC 1 cut(s) 403
BfaI CTAG 1 cut(s) 558
BisI GCNGC 5 cut(s) 264, 305, 405, 516, 689
BlsI GCNGC 5 cut(s) 265, 306, 406, 517, 690
Bme1390I CCNGG 2 cut(s) 172, 389
BmgT120I GGNCC 2 cut(s) 174, 437
BmiI GGNNCC 4 cut(s) 118, 231, 502, 680
BmrFI CCNGG 2 cut(s) 172, 389
BpiI GAAGAC 1 cut(s) 745
Bpu14I TTCGAA 1 cut(s) 77
BsaJI CCNNGG 5 cut(s) 30, 171, 388, 654, 743
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 35, 129, 183
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 331
Bse21I CCTNAGG 1 cut(s) 181
Bse3DI GCAATG 2 cut(s) 525, 637
BseBI CCWGG 2 cut(s) 172, 389
BseDI CCNNGG 5 cut(s) 30, 171, 388, 654, 743
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 4 cut(s) 32, 35, 129, 183
BseMI GCAATG 2 cut(s) 525, 637
BseMII CTCAG 2 cut(s) 195, 264
BseNI ACTGG 1 cut(s) 331
BseXI GCAGC 5 cut(s) 250, 291, 391, 502, 700
BshFI GGCC 4 cut(s) 176, 438, 524, 552
BshNI GGYRCC 1 cut(s) 229
BsiSI CCGG 2 cut(s) 435, 504
BslI CCNNNNNNNGG 4 cut(s) 32, 35, 129, 183
BsmAI GTCTC 1 cut(s) 403
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 4 cut(s) 176, 438, 524, 552
Bsp119I TTCGAA 1 cut(s) 77
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 2 cut(s) 86, 676
BspANI GGCC 4 cut(s) 176, 438, 524, 552
BspCNI CTCAG 2 cut(s) 194, 265
BspLI GGNNCC 4 cut(s) 118, 231, 502, 680
BspT104I TTCGAA 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 2 cut(s) 525, 637
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 1 cut(s) 331
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 5 cut(s) 30, 171, 388, 654, 743
BssMI GATC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 30, 654
Bst2UI CCWGG 2 cut(s) 172, 389
Bst4CI ACNGT 2 cut(s) 209, 317
BstAPI GCANNNNNTGC 1 cut(s) 260
BstBI TTCGAA 1 cut(s) 77
BstDEI CTNAG 2 cut(s) 181, 273
BstF5I GGATG 1 cut(s) 151
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 3 cut(s) 260, 310, 521
BstNI CCWGG 2 cut(s) 172, 389
BstSCI CCNGG 2 cut(s) 170, 387
BstV1I GCAGC 5 cut(s) 250, 291, 391, 502, 700
BstV2I GAAGAC 1 cut(s) 745
Bsu36I CCTNAGG 1 cut(s) 181
BsuRI GGCC 4 cut(s) 176, 438, 524, 552
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 2 cut(s) 285, 324
CaiI CAGNNNCTG 1 cut(s) 260
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 174, 437
Csp6I GTAC 2 cut(s) 541, 572
CspCI CAANNNNNGTGG 2 cut(s) 109, 144
CviAII CATG 4 cut(s) 226, 289, 395, 464
CviQI GTAC 2 cut(s) 541, 572
DdeI CTNAG 2 cut(s) 181, 273
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 522
Eco130I CCWWGG 2 cut(s) 30, 654
Eco147I AGGCCT 1 cut(s) 552
Eco32I GATATC 1 cut(s) 640
Eco81I CCTNAGG 1 cut(s) 181
EcoRI GAATTC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 170, 387
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 30, 654
ErhI CCWWGG 2 cut(s) 30, 654
FaeI CATG 4 cut(s) 229, 292, 398, 467
FatI CATG 4 cut(s) 225, 288, 394, 463
FblI GTMKAC 1 cut(s) 470
Fnu4HI GCNGC 5 cut(s) 264, 305, 405, 516, 689
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 5 cut(s) 264, 305, 405, 516, 689
FspBI CTAG 1 cut(s) 558
GluI GCNGC 5 cut(s) 264, 305, 405, 516, 689
HaeIII GGCC 4 cut(s) 176, 438, 524, 552
HapII CCGG 2 cut(s) 435, 504
Hin1II CATG 4 cut(s) 229, 292, 398, 467
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HinfI GANTC 2 cut(s) 335, 533
HpaII CCGG 2 cut(s) 435, 504
HphI GGTGA 3 cut(s) 349, 424, 723
Hpy166II GTNNAC 2 cut(s) 105, 471
Hpy188III TCNNGA 2 cut(s) 143, 698
Hpy8I GTNNAC 2 cut(s) 105, 471
HpyAV CCTTC 2 cut(s) 542, 560
HpyCH4III ACNGT 2 cut(s) 209, 317
HpyCH4V TGCA 6 cut(s) 307, 313, 407, 448, 518, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 310, 521
HpyF3I CTNAG 2 cut(s) 181, 273
Hsp92II CATG 4 cut(s) 229, 292, 398, 467
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 5 cut(s) 250, 291, 391, 502, 700
MaeI CTAG 1 cut(s) 558
MaeIII GTNAC 5 cut(s) 280, 317, 346, 383, 580
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 3 cut(s) 91, 520, 750
MfeI CAATTG 2 cut(s) 189, 308
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 5 cut(s) 135, 189, 216, 308, 668
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 8 cut(s) 36, 108, 190, 268, 365, 450, 580, 738
Mox20I TGGCCA 1 cut(s) 524
MscI TGGCCA 1 cut(s) 524
MseI TTAA 2 cut(s) 134, 754
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 2 cut(s) 435, 504
MspR9I CCNGG 2 cut(s) 172, 389
MunI CAATTG 2 cut(s) 189, 308
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 2 cut(s) 172, 389
MwoI GCNNNNNNNGC 3 cut(s) 260, 310, 521
NdeII GATC 1 cut(s) 49
NlaIII CATG 4 cut(s) 229, 292, 398, 467
NlaIV GGNNCC 4 cut(s) 118, 231, 502, 680
NmeAIII GCCGAG 1 cut(s) 380
NmuCI GTSAC 2 cut(s) 280, 346
NspV TTCGAA 1 cut(s) 77
PceI AGGCCT 1 cut(s) 552
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 1 cut(s) 335
PflFI GACNNNGTC 1 cut(s) 290
PkrI GCNGC 5 cut(s) 265, 306, 406, 517, 690
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
Psp6I CCWGG 2 cut(s) 170, 387
PspGI CCWGG 2 cut(s) 170, 387
PspN4I GGNNCC 4 cut(s) 118, 231, 502, 680
PspPI GGNCC 2 cut(s) 174, 437
PstNI CAGNNNCTG 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 304
RsaI GTAC 2 cut(s) 542, 573
RsaNI GTAC 2 cut(s) 541, 572
SalI GTCGAC 1 cut(s) 469
SaqAI TTAA 2 cut(s) 134, 754
SatI GCNGC 5 cut(s) 264, 305, 405, 516, 689
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 2 cut(s) 174, 437
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 2 cut(s) 172, 389
SfuI TTCGAA 1 cut(s) 77
Sse9I AATT 5 cut(s) 135, 189, 216, 308, 668
SseBI AGGCCT 1 cut(s) 552
SsiI CCGC 2 cut(s) 86, 676
SspMI CTAG 1 cut(s) 558
StuI AGGCCT 1 cut(s) 552
StyD4I CCNGG 2 cut(s) 170, 387
StyI CCWWGG 2 cut(s) 30, 654
TaaI ACNGT 2 cut(s) 209, 317
TaqI TCGA 4 cut(s) 77, 470, 531, 636
TasI AATT 5 cut(s) 135, 189, 216, 308, 668
TatI WGTACW 1 cut(s) 540
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 2 cut(s) 134, 754
Tru9I TTAA 2 cut(s) 134, 754
TscAI CASTG 2 cut(s) 285, 331
TseFI GTSAC 2 cut(s) 280, 346
TseI GCWGC 5 cut(s) 263, 304, 404, 515, 688
Tsp45I GTSAC 2 cut(s) 280, 346
TspDTI ATGAA 2 cut(s) 209, 579
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 2 cut(s) 285, 331
Tth111I GACNNNGTC 1 cut(s) 290
XapI RAATTY 2 cut(s) 216, 668
XmiI GTMKAC 1 cut(s) 470
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.