Rorug02G0571200

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001826
Physical Location & Seq
Forward (+)
69742272 .. 69749458
7187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0571200.1

Sequence Viewer

Length: 1383 bp
ATGGCAAACTCAAACTCACTCGTAAAAGTGGTGGAAGTTTGCAGGGTTGCTCCAAACCCAGGCTCACCGGACTCAGCCACACCAGATTCCCTTCCTCTAACCTTGTTCGATATACGTTGGTTAAGGTTTGCACCGGTGCAGCGCCTGTACTTTTATGAAATATCTTCTACTGACTCCATACTTCCCAAACTCAAAGCCTCCCTCTCTCTCACACTCCAACACTTTCTGCCCCTTGCTGGAAACCTCACTTGGCCCCAAGACTCCCTCAAACCCATTCTCTGTTATGCCGAAGGTGACACTGTTTCACTTACAGTAGCTGAATCTGCTGCTAATTTTGGCCATCTCTCAAGCAAGTACGACTTGCTTGGAGTCCAAGAATACCATCCTCTTGTACCCCAGTTGGAAGTATCTCATGAACGATCTGCAGTAATGGCATTGCAAATCACTATCTTTCCCAACCGAGGCTTTTCCATTGGAACATCAATGCACCATGCTGTCTTGGATGGTAAAACTTCAACCTCCTTCCTTAAATCATGGGCTCACATATGCAAACATGAAGAACTGGGATCTTCATCTAGTTTGTTACCAGATGAGCTAAAGCCATTTTATGACAGAATGGTCATCCAAGACTTGGCAGAGCTTGGGGCACTCTACTCGGACCAATACCAAAACATGGATGGCCCCAACAATAGAAGCTTGATGGTTTGGAATTTTCAAGCTCCACCAGAGTCAATTCGAGGCACCTTCAAAGTCTGTCATGCAAAAATACAAAAACTAAGGCAACATGTCATGACTAGGATGGTAAAGAAGAAACAACATGATGATTCAGAAGCTTCAGTACTTCGTTTATCAACGTTTTCTCTAGCATGTGCCTATACATGGGTTTGCTTAGTCAAAGCTCAAGAAGCCAAAGAAGATAAAACACGACTCATATTTCCAGTGGACTGTAGGTCTCGCTTAGACCCTCCTATACCTGCAACATATTTTGGTAACTGCATAGCAGGCCGTTTGGCAATTGCAGAAACAAAAGGGCTATTGGGAGAAGATGGATTAGTTGTGGCCGTGAGTGCAATTATTGAAGCTATAAAAGGTTTGGATGATGGAGTCTTGAATGGGGCAGAGAATTGGGTTTCAAGATTGTGCACTGTGCTGAGTGATAGAGTAGCTTCCATTGCTGGCTCACATCGGTTTGGTGTTTATGAAACTGATTTTGGATGGGGAATACCAAAAAAGGTCGATCTTGTTTCTATAGATAGGACAGGAGCAATCTCCCTTTCAGATACCAATGATAGTGATGGAGGTTTGGACGTTGGATTGGTTTTACAAAAACATCATACAGAAGTTTTTGCTTCTCTATTTGCCAAAGGTCTTGAGAACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

460

Amino Acids

50.57

Weight (kDa)

6.07

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 26 - 451 7.5e-49 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
Acc36I ACCTGC 1 cut(s) 982
AccB1I GGYRCC 1 cut(s) 740
AccB7I CCANNNNNTGG 2 cut(s) 631, 673
AclI AACGTT 1 cut(s) 854
AclWI GGATC 1 cut(s) 574
AcoI YGGCCR 2 cut(s) 337, 1059
AcsI RAATTY 1 cut(s) 709
AcuI CTGAAG 1 cut(s) 819
AfaI GTAC 4 cut(s) 149, 356, 393, 840
AfiI CCNNNNNNNGG 6 cut(s) 59, 236, 461, 631, 673, 879
AflIII ACRYGT 1 cut(s) 784
AgeI ACCGGT 1 cut(s) 133
AgsI TTSAA 6 cut(s) 516, 716, 748, 1079, 1111, 1134
AhdI GACNNNNNGTC 1 cut(s) 949
AjnI CCWGG 1 cut(s) 58
AjuI GAANNNNNNNTTGG 4 cut(s) 232, 264, 1194, 1226
AluBI AGCT 9 cut(s) 317, 595, 640, 696, 719, 833, 899, 1082, 1166
AluI AGCT 9 cut(s) 317, 595, 640, 696, 719, 833, 899, 1082, 1166
Alw21I GWGCWC 1 cut(s) 1145
Alw26I GTCTC 1 cut(s) 957
Alw44I GTGCAC 1 cut(s) 1141
AlwI GGATC 1 cut(s) 574
AlwNI CAGNNNCTG 2 cut(s) 145, 317
AoxI GGCC 5 cut(s) 251, 337, 679, 1003, 1059
ApaLI GTGCAC 1 cut(s) 1141
ApeKI GCWGC 2 cut(s) 139, 326
ApoI RAATTY 1 cut(s) 709
AsiGI ACCGGT 1 cut(s) 133
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 3 cut(s) 252, 658, 680
AsuHPI GGTGA 2 cut(s) 57, 305
AvaII GGWCC 1 cut(s) 658
BaeGI GKGCMC 2 cut(s) 649, 1145
BalI TGGCCA 1 cut(s) 339
BanI GGYRCC 1 cut(s) 740
BanII GRGCYC 1 cut(s) 541
BarI GAAGNNNNNNTAC 2 cut(s) 822, 854
Bbv12I GWGCWC 1 cut(s) 1145
BbvI GCAGC 2 cut(s) 151, 313
BceAI ACGGC 2 cut(s) 990, 1046
BcgI CGANNNNNNTGC 2 cut(s) 636, 670
BciT130I CCWGG 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 957
BfaI CTAG 3 cut(s) 576, 795, 863
BfmI CTRYAG 3 cut(s) 423, 946, 1248
BfoI RGCGCY 1 cut(s) 145
BfuAI ACCTGC 1 cut(s) 982
BisI GCNGC 2 cut(s) 140, 327
BlsI GCNGC 2 cut(s) 141, 328
BmcAI AGTACT 1 cut(s) 840
Bme1390I CCNGG 1 cut(s) 60
Bme18I GGWCC 1 cut(s) 658
BmeRI GACNNNNNGTC 1 cut(s) 949
BmgT120I GGNCC 3 cut(s) 252, 658, 680
BmiI GGNNCC 3 cut(s) 254, 682, 742
BmrFI CCNGG 1 cut(s) 60
BmrI ACTGGG 2 cut(s) 391, 572
BmuI ACTGGG 2 cut(s) 391, 572
BpuEI CTTGAG 2 cut(s) 331, 885
BsaBI GATNNNNATC 1 cut(s) 571
BsaI GGTCTC 1 cut(s) 957
BsaJI CCNNGG 2 cut(s) 58, 460
BsaWI WCCGGW 2 cut(s) 67, 133
Bsc4I CCNNNNNNNGG 6 cut(s) 59, 236, 461, 631, 673, 879
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 3 cut(s) 397, 567, 938
Bse3DI GCAATG 2 cut(s) 434, 1170
Bse8I GATNNNNATC 1 cut(s) 571
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 2 cut(s) 58, 460
BseGI GGATG 7 cut(s) 382, 508, 621, 682, 804, 1102, 1220
BseJI GATNNNNATC 1 cut(s) 571
BseLI CCNNNNNNNGG 6 cut(s) 59, 236, 461, 631, 673, 879
BseMI GCAATG 2 cut(s) 434, 1170
BseMII CTCAG 2 cut(s) 87, 1142
BseNI ACTGG 3 cut(s) 397, 567, 938
BseSI GKGCMC 2 cut(s) 649, 1145
BseXI GCAGC 2 cut(s) 151, 313
BsgI GTGCAG 1 cut(s) 158
BshFI GGCC 5 cut(s) 253, 339, 681, 1005, 1061
BshNI GGYRCC 1 cut(s) 740
BshTI ACCGGT 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 1145
BsiSI CCGG 2 cut(s) 68, 134
BslI CCNNNNNNNGG 6 cut(s) 59, 236, 461, 631, 673, 879
BsmAI GTCTC 1 cut(s) 957
BsnI GGCC 5 cut(s) 253, 339, 681, 1005, 1061
Bso31I GGTCTC 1 cut(s) 957
Bsp1286I GDGCHC 3 cut(s) 541, 649, 1145
Bsp143I GATC 3 cut(s) 419, 566, 1237
BspANI GGCC 5 cut(s) 253, 339, 681, 1005, 1061
BspCNI CTCAG 2 cut(s) 86, 1143
BspHI TCATGA 2 cut(s) 412, 789
BspLI GGNNCC 3 cut(s) 254, 682, 742
BspMAI CTGCAG 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 982
BspPI GGATC 1 cut(s) 574
BspT107I GGYRCC 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 957
BsrDI GCAATG 2 cut(s) 434, 1170
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 3 cut(s) 397, 567, 938
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 58, 460
BssMI GATC 3 cut(s) 419, 566, 1237
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 4 cut(s) 301, 313, 947, 1147
BstC8I GCNNGC 2 cut(s) 1003, 1177
BstDEI CTNAG 5 cut(s) 73, 776, 889, 958, 1151
BstF5I GGATG 7 cut(s) 382, 508, 621, 682, 804, 1102, 1220
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 3 cut(s) 422, 569, 1240
BstMAI GTCTC 1 cut(s) 957
BstMBI GATC 3 cut(s) 419, 566, 1237
BstMWI GCNNNNNNNGC 6 cut(s) 323, 431, 905, 1002, 1067, 1172
BstNI CCWGG 1 cut(s) 60
BstNSI RCATGY 2 cut(s) 788, 870
BstSCI CCNGG 1 cut(s) 58
BstSFI CTRYAG 3 cut(s) 423, 946, 1248
BstSLI GKGCMC 2 cut(s) 649, 1145
BstV1I GCAGC 2 cut(s) 151, 313
BstX2I RGATCY 1 cut(s) 566
BstYI RGATCY 1 cut(s) 566
BsuRI GGCC 5 cut(s) 253, 339, 681, 1005, 1061
BtsCI GGATG 7 cut(s) 382, 508, 621, 682, 804, 1102, 1220
BtsIMutI CAGTG 3 cut(s) 297, 945, 1143
BveI ACCTGC 1 cut(s) 982
Cac8I GCNNGC 2 cut(s) 1003, 1177
CaiI CAGNNNCTG 2 cut(s) 145, 317
CciI TCATGA 2 cut(s) 412, 789
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 252, 658, 680
Csp6I GTAC 4 cut(s) 148, 355, 392, 839
CspAI ACCGGT 1 cut(s) 133
CviQI GTAC 4 cut(s) 148, 355, 392, 839
DdeI CTNAG 5 cut(s) 73, 776, 889, 958, 1151
DpnI GATC 3 cut(s) 421, 568, 1239
DpnII GATC 3 cut(s) 419, 566, 1237
DrdI GACNNNNNNGTC 1 cut(s) 617
DriI GACNNNNNGTC 1 cut(s) 949
DseDI GACNNNNNNGTC 1 cut(s) 617
EaeI YGGCCR 2 cut(s) 337, 1059
Eam1105I GACNNNNNGTC 1 cut(s) 949
Eco24I GRGCYC 1 cut(s) 541
Eco31I GGTCTC 1 cut(s) 957
Eco47I GGWCC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 819
EcoRII CCWGG 1 cut(s) 58
EcoT38I GRGCYC 1 cut(s) 541
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FauNDI CATATG 1 cut(s) 545
Fnu4HI GCNGC 2 cut(s) 140, 327
FokI GGATG 7 cut(s) 369, 515, 608, 689, 811, 1109, 1227
FriOI GRGCYC 1 cut(s) 541
Fsp4HI GCNGC 2 cut(s) 140, 327
FspBI CTAG 3 cut(s) 576, 795, 863
GlaI GCGC 1 cut(s) 143
GluI GCNGC 2 cut(s) 140, 327
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 5 cut(s) 253, 339, 681, 1005, 1061
HapII CCGG 2 cut(s) 68, 134
HhaI GCGC 1 cut(s) 144
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HindIII AAGCTT 2 cut(s) 694, 831
HpaII CCGG 2 cut(s) 68, 134
HphI GGTGA 2 cut(s) 57, 305
Hpy166II GTNNAC 2 cut(s) 943, 1143
Hpy188I TCNGA 3 cut(s) 658, 829, 1279
Hpy188III TCNNGA 6 cut(s) 413, 790, 902, 1108, 1134, 1370
Hpy8I GTNNAC 2 cut(s) 943, 1143
HpyAV CCTTC 4 cut(s) 101, 284, 532, 754
HpyCH4III ACNGT 4 cut(s) 301, 313, 947, 1147
HpyCH4IV ACGT 3 cut(s) 115, 854, 1308
HpyF10VI GCNNNNNNNGC 6 cut(s) 323, 431, 905, 1002, 1067, 1172
HpyF3I CTNAG 5 cut(s) 73, 776, 889, 958, 1151
HpySE526I ACGT 3 cut(s) 115, 854, 1308
HspAI GCGC 1 cut(s) 142
Kzo9I GATC 3 cut(s) 419, 566, 1237
LmnI GCTCC 3 cut(s) 55, 724, 1262
Lsp1109I GCAGC 2 cut(s) 151, 313
MaeI CTAG 3 cut(s) 576, 795, 863
MaeII ACGT 3 cut(s) 115, 854, 1308
MaeIII GTNAC 3 cut(s) 293, 582, 989
MalI GATC 3 cut(s) 421, 568, 1239
MboI GATC 3 cut(s) 419, 566, 1237
MboII GAAGA 6 cut(s) 156, 561, 569, 820, 926, 1055
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 1 cut(s) 566
MhlI GDGCHC 3 cut(s) 541, 649, 1145
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 6 cut(s) 331, 709, 732, 1014, 1071, 1123
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 7 cut(s) 65, 167, 254, 378, 737, 921, 1113
MmeI TCCRAC 3 cut(s) 241, 381, 1291
Mox20I TGGCCA 1 cut(s) 339
MscI TGGCCA 1 cut(s) 339
MseI TTAA 2 cut(s) 122, 528
Msp20I TGGCCA 1 cut(s) 339
MspI CCGG 2 cut(s) 68, 134
MspR9I CCNGG 1 cut(s) 60
MunI CAATTG 1 cut(s) 1014
MvaI CCWGG 1 cut(s) 60
MwoI GCNNNNNNNGC 6 cut(s) 323, 431, 905, 1002, 1067, 1172
NdeI CATATG 1 cut(s) 545
NdeII GATC 3 cut(s) 419, 566, 1237
NlaIV GGNNCC 3 cut(s) 254, 682, 742
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 2 cut(s) 788, 870
PagI TCATGA 2 cut(s) 412, 789
PciI ACATGT 1 cut(s) 784
PfeI GAWTC 3 cut(s) 86, 320, 824
PflMI CCANNNNNTGG 2 cut(s) 631, 673
PinAI ACCGGT 1 cut(s) 133
PkrI GCNGC 2 cut(s) 141, 328
PleI GAGTC 7 cut(s) 65, 167, 254, 377, 736, 921, 1112
PpsI GAGTC 7 cut(s) 65, 167, 254, 377, 736, 921, 1112
PscI ACATGT 1 cut(s) 784
Psp1406I AACGTT 1 cut(s) 854
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PspN4I GGNNCC 3 cut(s) 254, 682, 742
PspPI GGNCC 3 cut(s) 252, 658, 680
PstI CTGCAG 1 cut(s) 427
PstNI CAGNNNCTG 2 cut(s) 145, 317
PsuI RGATCY 1 cut(s) 566
RsaI GTAC 4 cut(s) 149, 356, 393, 840
RsaNI GTAC 4 cut(s) 148, 355, 392, 839
SaqAI TTAA 2 cut(s) 122, 528
SatI GCNGC 2 cut(s) 140, 327
Sau3AI GATC 3 cut(s) 419, 566, 1237
Sau96I GGNCC 3 cut(s) 252, 658, 680
ScaI AGTACT 1 cut(s) 840
SchI GAGTC 7 cut(s) 65, 167, 254, 378, 737, 921, 1113
ScrFI CCNGG 1 cut(s) 60
SduI GDGCHC 3 cut(s) 541, 649, 1145
SfcI CTRYAG 3 cut(s) 423, 946, 1248
SgrAI CRCCGGYG 1 cut(s) 133
SinI GGWCC 1 cut(s) 658
SmlI CTYRAG 3 cut(s) 346, 900, 1370
SmoI CTYRAG 3 cut(s) 346, 900, 1370
Sse9I AATT 6 cut(s) 331, 709, 732, 1014, 1071, 1123
SspMI CTAG 3 cut(s) 576, 795, 863
StyD4I CCNGG 1 cut(s) 58
TaaI ACNGT 4 cut(s) 301, 313, 947, 1147
TaiI ACGT 3 cut(s) 118, 857, 1311
TaqI TCGA 3 cut(s) 108, 736, 1236
TasI AATT 6 cut(s) 331, 709, 732, 1014, 1071, 1123
TatI WGTACW 2 cut(s) 147, 838
TfiI GAWTC 3 cut(s) 86, 320, 824
Tru1I TTAA 2 cut(s) 122, 528
Tru9I TTAA 2 cut(s) 122, 528
TscAI CASTG 3 cut(s) 304, 945, 1150
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 2 cut(s) 139, 326
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 5 cut(s) 171, 429, 561, 570, 1215
TspRI CASTG 3 cut(s) 304, 945, 1150
Van91I CCANNNNNTGG 2 cut(s) 631, 673
VneI GTGCAC 1 cut(s) 1141
VpaK11BI GGWCC 1 cut(s) 658
XapI RAATTY 1 cut(s) 709
XceI RCATGY 2 cut(s) 788, 870
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XspI CTAG 3 cut(s) 576, 795, 863
ZrmI AGTACT 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.