Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001826

Physical Location & Seq

Genomic Coordinates Forward (+)

10473591 .. 10477981

Gene Structure 4391 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1120200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGGCAGGAAGCACTTTTTGGCTTGTTCTTCTAATTGGGGTTTTTGGCTTTCTTTTGGTTCTAATTCTAAACCACTTCTTGCCCTTAATGTTGTTAAGGCTTAATGGTACTCTTCCGCCTAAGGGCTCTTTTGGGTGGCCTCTGTTGGGAGAAACTCTTAGCTTCTTGAAGCCTCATCCTTCTAATTCTCTTGGTGCCTTCCTTAAACACCATTGTTCTAGGTATGGGAAAGTGTTCAAATCCCATTTGTTCTTGTCTCCCACAATTGTGTCATGTGATCAAGAGCTGAACTACTTCATACTTCAGAATGAAGGTAAGTTGTTTGAATGCAGCTATCCAAAGCCTATCCATGGCATCCTTGGAAAATCCTCCATGCTTGTGGCTGTAGGTGACACTCACAAAAGGCTCAGAAATGTGGCTGTCTCCTTGGTCACCATCACCAAGACCAAGCCTGAGTTTCTTAGTGACATTGAAGCCACAGCCATTGGCATACTTCACTCTTGGAAAGATAAATCAAGAGTCATCTTCTGTGAGGAAGCCAGAAAGTTCACATTCAATGTAATAGTGAAGCAAGTGCTAGGTTTAACCCCAGTTGAGCCACAGGCTACACAAATTCTTGAAGATTTCCTGACTTTCATGAGAGGGCTCATTTCTCTTCCTCTCTATATTCCTGGAACCCCATATGCAAGAGCTGTTAAGGCTAGAAGCAGGATATCTTCAACTGTGAAAGCAATCATAGAGGAAAGAAGAAGAAATGCAGATGGGAGCACGAATTCTTGTACTAAAAGAAGTGATTTCCTTGAGATACTTCTGGATGTTGATTCCTTGTCTGAAGATGAGAAAGTGAGTTTTATCTTGGATTCTCTATTGGGTGGCTATGAAACCACCTCTCTCTTGATGGCCATGGTAGTTTATTTTCTAGCCCAATCACCATCTGCATTACAACGATTAAAGGTAGAGCATCAGAACATTAGAAGAGTGAAGCAGAAAGATGAGTATCTGAACTGGGACGACTATAAAAAAATGGAATTCACCCAAAATGTCATTAACGAAGCTCTCAGATGTGGAAACGTTGTAAAATTCGTGCACCGAAAGGCTCTCAAAGATGTAAAATTTAGAGATTATTTAATTCCATCTGGCTGGAAAGTTTTACCAGTTTTTAGTGCAGCTCATTTAGACCCTTCTCTTCATGCAAGTGCTCTTGAGTTTGATCCTTGGAGATGGGAGAAAATACCAAGCCAAGACCATGTATGCAAGAAATTTACTCCCTTCGGTGGAGGGTCTAGATGCTGTCCTGGATCTGAACTTGGGAAGCTTGAAGTTGCTGTTTTTCTCCACCACCTTGTACAGAATTTCAGGTGGAGGACCGAGGACGACGACGAACCCGTAGCGTTTCCATACGTAGAATTTCAAAGAGGATTGCCACTGTACCTGGAGCATTGCCCCCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005488 GO:0006629 GO:0006694 GO:0007275 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009791 GO:0010268 GO:0010817 GO:0016125 GO:0016128 GO:0016129 GO:0016131 GO:0016132 GO:0016491 GO:0020037 GO:0032501 GO:0032502 GO:0042445 GO:0042446 GO:0042592 GO:0044238 GO:0046906 GO:0048037 GO:0048856 GO:0048878 GO:0050896 GO:0055088 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0097159 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00905 ko01100 ko01110 map00905 map01100 map01110

Pfam Domains

Protein Families

p450

Protein Analysis

484

Amino Acids

54.87

Weight (kDa)

8.84

Isoelectric Point (pI)

45.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	40 - 462	8.6e-60	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001826)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14400 AT5G14400 AT5G14400 AT5G14400
fragaria_vesca	FvH4_6g40310 FvH4_6g40310 FvH4_6g40310 FvH4_6g40310 FvH4_6g52040
malus_domestica	MD09G1020600.v1.1 MD09G1020700.v1.1 MD17G1120200.v1.1
prunus_persica	Prupe.3G147200_v2.0.a1 Prupe.3G193900_v2.0.a1 Prupe.3G193900_v2.0.a1 Prupe.3G295600_v2.0.a1
pyrus_communis	pycom111g01730
rosa_chinensis	RchiOBHm_Chr2g0155501 RchiOBHm_Chr2g0173551
rosa_laevigata	RLG00000020841 RLG00000022201
rosa_multiflora	Rmu_co8071130.1_g000001 Rmu_sc0001045.1_g000022 Rmu_sc0001046.1_g000003 Rmu_sc0002538.1_g000025 Rmu_sc0003439.1_g000011 Rmu_sc0029872.1_g000004
rosa_roxburghii	Rroxscaffold_2G00078790 Rroxscaffold_2G00093660
rosa_rugosa	Rorug02G0447300 Rorug02G0447400 Rorug02G0571200 Rorug02G0571300
rosa_samantha	Rh2AG513800 Rh2AG650000 Rh2BG524700 Rh2BG660400 Rh2CG498700 Rh2CG625400 Rh2DG533700 Rh2DG675800 Rh5AG197900 Rh5BG196500 Rh5BG196600
rosa_wichuraiana	Rw2G042230 Rw2G053350 Rw5G018070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	194
AciI	CCGC	1 cut(s)	116
AclI	AACGTT	1 cut(s)	1071
AclWI	GGATC	2 cut(s)	1205, 1306
AcoI	YGGCCR	1 cut(s)	900
AcsI	RAATTY	8 cut(s)	612, 772, 1028, 1079, 1112, 1259, 1351, 1406
AcuI	CTGAAG	2 cut(s)	287, 852
AfaI	GTAC	4 cut(s)	109, 781, 1347, 1430
AfiI	CCNNNNNNNGG	4 cut(s)	122, 146, 350, 1274
AgsI	TTSAA	9 cut(s)	169, 238, 326, 473, 556, 620, 720, 1319, 1412
AjnI	CCWGG	3 cut(s)	670, 1294, 1431
AjuI	GAANNNNNNNTTGG	1 cut(s)	33
AleI	CACNNNNGTG	1 cut(s)	266
AluBI	AGCT	7 cut(s)	162, 286, 333, 692, 1055, 1169, 1315
AluI	AGCT	7 cut(s)	162, 286, 333, 692, 1055, 1169, 1315
Alw21I	GWGCWC	3 cut(s)	770, 1089, 1201
Alw26I	GTCTC	2 cut(s)	261, 427
Alw44I	GTGCAC	1 cut(s)	1085
AlwI	GGATC	2 cut(s)	1205, 1306
AoxI	GGCC	2 cut(s)	137, 900
ApaLI	GTGCAC	1 cut(s)	1085
ApeKI	GCWGC	2 cut(s)	330, 1166
ApoI	RAATTY	8 cut(s)	612, 772, 1028, 1079, 1112, 1259, 1351, 1406
Asp700I	GAANNNNTTC	2 cut(s)	233, 293
AspS9I	GGNCC	1 cut(s)	1365
AsuHPI	GGTGA	5 cut(s)	401, 424, 430, 921, 1024
AvaII	GGWCC	1 cut(s)	1365
AxyI	CCTNAGG	1 cut(s)	120
BaeGI	GKGCMC	1 cut(s)	1089
BalI	TGGCCA	1 cut(s)	902
BanI	GGYRCC	1 cut(s)	194
BanII	GRGCYC	2 cut(s)	128, 648
Bbv12I	GWGCWC	3 cut(s)	770, 1089, 1201
BbvI	GCAGC	2 cut(s)	342, 1178
BccI	CCATC	6 cut(s)	443, 755, 892, 940, 1141, 1215
BciT130I	CCWGG	3 cut(s)	672, 1296, 1433
BclI	TGATCA	1 cut(s)	277
BcoDI	GTCTC	2 cut(s)	261, 427
BfaI	CTAG	5 cut(s)	219, 578, 702, 920, 1284
BfmI	CTRYAG	1 cut(s)	384
BisI	GCNGC	2 cut(s)	331, 1167
BlsI	GCNGC	2 cut(s)	332, 1168
Bme1390I	CCNGG	3 cut(s)	672, 1296, 1433
Bme18I	GGWCC	1 cut(s)	1365
BmgT120I	GGNCC	1 cut(s)	1365
BmiI	GGNNCC	2 cut(s)	196, 676
BmrFI	CCNGG	3 cut(s)	672, 1296, 1433
BmrI	ACTGGG	2 cut(s)	584, 1015
BmsI	GCATC	3 cut(s)	363, 970, 1277
BmuI	ACTGGG	2 cut(s)	584, 1015
BpuEI	CTTGAG	2 cut(s)	821, 1223
BsaAI	YACGTR	1 cut(s)	1402
BsaBI	GATNNNNATC	1 cut(s)	996
BsaJI	CCNNGG	6 cut(s)	349, 358, 426, 903, 1214, 1368
Bsc4I	CCNNNNNNNGG	4 cut(s)	122, 146, 350, 1274
Bse1I	ACTGG	3 cut(s)	590, 1010, 1154
Bse21I	CCTNAGG	1 cut(s)	120
Bse3DI	GCAATG	1 cut(s)	1438
Bse8I	GATNNNNATC	1 cut(s)	996
BseBI	CCWGG	3 cut(s)	672, 1296, 1433
BseDI	CCNNGG	6 cut(s)	349, 358, 426, 903, 1214, 1368
BseGI	GGATG	3 cut(s)	175, 354, 820
BseJI	GATNNNNATC	1 cut(s)	996
BseLI	CCNNNNNNNGG	4 cut(s)	122, 146, 350, 1274
BseMI	GCAATG	1 cut(s)	1438
BseMII	CTCAG	3 cut(s)	421, 444, 1072
BseNI	ACTGG	3 cut(s)	590, 1010, 1154
BseSI	GKGCMC	1 cut(s)	1089
BseXI	GCAGC	2 cut(s)	342, 1178
BsgI	GTGCAG	1 cut(s)	1185
BshFI	GGCC	2 cut(s)	139, 902
BshNI	GGYRCC	1 cut(s)	194
BsiHKAI	GWGCWC	3 cut(s)	770, 1089, 1201
BslFI	GGGAC	1 cut(s)	1022
BslI	CCNNNNNNNGG	4 cut(s)	122, 146, 350, 1274
BsmAI	GTCTC	2 cut(s)	261, 427
BsmFI	GGGAC	1 cut(s)	1022
BsmI	GAATGC	1 cut(s)	332
BsnI	GGCC	2 cut(s)	139, 902
Bsp1286I	GDGCHC	5 cut(s)	128, 648, 770, 1089, 1201
Bsp1407I	TGTACA	1 cut(s)	1345
Bsp143I	GATC	3 cut(s)	277, 1210, 1298
Bsp19I	CCATGG	2 cut(s)	349, 903
BspACI	CCGC	1 cut(s)	116
BspANI	GGCC	2 cut(s)	139, 902
BspCNI	CTCAG	3 cut(s)	420, 445, 1071
BspHI	TCATGA	1 cut(s)	636
BspLI	GGNNCC	2 cut(s)	196, 676
BspPI	GGATC	2 cut(s)	1205, 1306
BspT107I	GGYRCC	1 cut(s)	194
BsrDI	GCAATG	1 cut(s)	1438
BsrGI	TGTACA	1 cut(s)	1345
BsrI	ACTGG	3 cut(s)	590, 1010, 1154
BssECI	CCNNGG	6 cut(s)	349, 358, 426, 903, 1214, 1368
BssMI	GATC	3 cut(s)	277, 1210, 1298
BssT1I	CCWWGG	5 cut(s)	349, 358, 426, 903, 1214
Bst2UI	CCWGG	3 cut(s)	672, 1296, 1433
Bst4CI	ACNGT	2 cut(s)	724, 1428
Bst6I	CTCTTC	4 cut(s)	117, 660, 970, 1191
BstAUI	TGTACA	1 cut(s)	1345
BstBAI	YACGTR	1 cut(s)	1402
BstDEI	CTNAG	6 cut(s)	120, 158, 407, 453, 461, 1058
BstDSI	CCRYGG	2 cut(s)	349, 903
BstEII	GGTNACC	1 cut(s)	430
BstF5I	GGATG	3 cut(s)	175, 354, 820
BstKTI	GATC	3 cut(s)	280, 1213, 1301
BstMAI	GTCTC	2 cut(s)	261, 427
BstMBI	GATC	3 cut(s)	277, 1210, 1298
BstMWI	GCNNNNNNNGC	1 cut(s)	698
BstNI	CCWGG	3 cut(s)	672, 1296, 1433
BstPI	GGTNACC	1 cut(s)	430
BstSCI	CCNGG	3 cut(s)	670, 1294, 1431
BstSFI	CTRYAG	1 cut(s)	384
BstSLI	GKGCMC	1 cut(s)	1089
BstSNI	TACGTA	1 cut(s)	1402
BstV1I	GCAGC	2 cut(s)	342, 1178
BstX2I	RGATCY	1 cut(s)	1298
BstXI	CCANNNNNNTGG	2 cut(s)	379, 1140
BstYI	RGATCY	1 cut(s)	1298
Bsu36I	CCTNAGG	1 cut(s)	120
BsuRI	GGCC	2 cut(s)	139, 902
BtgI	CCRYGG	2 cut(s)	349, 903
BtsCI	GGATG	3 cut(s)	175, 354, 820
BtsIMutI	CAGTG	1 cut(s)	1424
CciI	TCATGA	1 cut(s)	636
Cfr13I	GGNCC	1 cut(s)	1365
Csp6I	GTAC	4 cut(s)	108, 780, 1346, 1429
CspCI	CAANNNNNGTGG	2 cut(s)	466, 501
CviAII	CATG	7 cut(s)	273, 350, 373, 637, 904, 1190, 1247
CviQI	GTAC	4 cut(s)	108, 780, 1346, 1429
DdeI	CTNAG	6 cut(s)	120, 158, 407, 453, 461, 1058
DpnI	GATC	3 cut(s)	279, 1212, 1300
DpnII	GATC	3 cut(s)	277, 1210, 1298
EaeI	YGGCCR	1 cut(s)	900
Eam1104I	CTCTTC	4 cut(s)	117, 660, 970, 1191
EarI	CTCTTC	4 cut(s)	117, 660, 970, 1191
EciI	GGCGGA	1 cut(s)	105
Eco105I	TACGTA	1 cut(s)	1402
Eco130I	CCWWGG	5 cut(s)	349, 358, 426, 903, 1214
Eco24I	GRGCYC	2 cut(s)	128, 648
Eco32I	GATATC	1 cut(s)	714
Eco47I	GGWCC	1 cut(s)	1365
Eco57I	CTGAAG	2 cut(s)	287, 852
Eco81I	CCTNAGG	1 cut(s)	120
Eco91I	GGTNACC	1 cut(s)	430
EcoO65I	GGTNACC	1 cut(s)	430
EcoRI	GAATTC	2 cut(s)	772, 1028
EcoRII	CCWGG	3 cut(s)	670, 1294, 1431
EcoRV	GATATC	1 cut(s)	714
EcoT14I	CCWWGG	5 cut(s)	349, 358, 426, 903, 1214
EcoT38I	GRGCYC	2 cut(s)	128, 648
ErhI	CCWWGG	5 cut(s)	349, 358, 426, 903, 1214
FaeI	CATG	7 cut(s)	276, 353, 376, 640, 907, 1193, 1250
FaqI	GGGAC	1 cut(s)	1022
FatI	CATG	7 cut(s)	272, 349, 372, 636, 903, 1189, 1246
FauNDI	CATATG	1 cut(s)	682
FbaI	TGATCA	1 cut(s)	277
Fnu4HI	GCNGC	2 cut(s)	331, 1167
FokI	GGATG	3 cut(s)	162, 341, 827
FriOI	GRGCYC	2 cut(s)	128, 648
Fsp4HI	GCNGC	2 cut(s)	331, 1167
FspBI	CTAG	5 cut(s)	219, 578, 702, 920, 1284
GluI	GCNGC	2 cut(s)	331, 1167
HaeIII	GGCC	2 cut(s)	139, 902
Hin1II	CATG	7 cut(s)	276, 353, 376, 640, 907, 1193, 1250
HindIII	AAGCTT	1 cut(s)	1313
HinfI	GANTC	3 cut(s)	519, 821, 860
HphI	GGTGA	5 cut(s)	401, 424, 430, 921, 1024
Hpy166II	GTNNAC	2 cut(s)	549, 1087
Hpy188I	TCNGA	7 cut(s)	306, 410, 832, 966, 1002, 1061, 1303
Hpy8I	GTNNAC	2 cut(s)	549, 1087
Hpy99I	CGWCG	2 cut(s)	1379, 1382
HpyAV	CCTTC	5 cut(s)	189, 208, 305, 1191, 1279
HpyCH4III	ACNGT	2 cut(s)	724, 1428
HpyCH4IV	ACGT	2 cut(s)	1071, 1401
HpyCH4V	TGCA	8 cut(s)	330, 686, 758, 938, 1087, 1166, 1193, 1254
HpyF10VI	GCNNNNNNNGC	1 cut(s)	698
HpyF3I	CTNAG	6 cut(s)	120, 158, 407, 453, 461, 1058
HpySE526I	ACGT	2 cut(s)	1071, 1401
Hsp92II	CATG	7 cut(s)	276, 353, 376, 640, 907, 1193, 1250
Ksp22I	TGATCA	1 cut(s)	277
Kzo9I	GATC	3 cut(s)	277, 1210, 1298
LmnI	GCTCC	2 cut(s)	765, 1435
Lsp1109I	GCAGC	2 cut(s)	342, 1178
LweI	GCATC	3 cut(s)	363, 970, 1277
MaeI	CTAG	5 cut(s)	219, 578, 702, 920, 1284
MaeII	ACGT	2 cut(s)	1071, 1401
MaeIII	GTNAC	3 cut(s)	389, 430, 464
MalI	GATC	3 cut(s)	279, 1212, 1300
MboI	GATC	3 cut(s)	277, 1210, 1298
MfeI	CAATTG	1 cut(s)	264
MflI	RGATCY	1 cut(s)	1298
MhlI	GDGCHC	5 cut(s)	128, 648, 770, 1089, 1201
MlsI	TGGCCA	1 cut(s)	902
MluNI	TGGCCA	1 cut(s)	902
MlyI	GAGTC	1 cut(s)	528
Mox20I	TGGCCA	1 cut(s)	902
MroXI	GAANNNNTTC	2 cut(s)	233, 293
MscI	TGGCCA	1 cut(s)	902
MslI	CAYNNNNRTG	3 cut(s)	266, 377, 1194
Msp20I	TGGCCA	1 cut(s)	902
MspR9I	CCNGG	3 cut(s)	672, 1296, 1433
MunI	CAATTG	1 cut(s)	264
Mva1269I	GAATGC	1 cut(s)	332
MvaI	CCWGG	3 cut(s)	672, 1296, 1433
MwoI	GCNNNNNNNGC	1 cut(s)	698
NcoI	CCATGG	2 cut(s)	349, 903
NdeI	CATATG	1 cut(s)	682
NdeII	GATC	3 cut(s)	277, 1210, 1298
NlaIII	CATG	7 cut(s)	276, 353, 376, 640, 907, 1193, 1250
NlaIV	GGNNCC	2 cut(s)	196, 676
NmuCI	GTSAC	3 cut(s)	389, 430, 464
OliI	CACNNNNGTG	1 cut(s)	266
PagI	TCATGA	1 cut(s)	636
PcsI	WCGNNNNNNNCGW	1 cut(s)	1383
PctI	GAATGC	1 cut(s)	332
PdmI	GAANNNNTTC	2 cut(s)	233, 293
PfeI	GAWTC	2 cut(s)	821, 860
PfoI	TCCNGGA	2 cut(s)	670, 1294
PkrI	GCNGC	2 cut(s)	332, 1168
PleI	GAGTC	1 cut(s)	527
PpsI	GAGTC	1 cut(s)	527
Ppu21I	YACGTR	1 cut(s)	1402
Psp1406I	AACGTT	1 cut(s)	1071
Psp6I	CCWGG	3 cut(s)	670, 1294, 1431
PspEI	GGTNACC	1 cut(s)	430
PspGI	CCWGG	3 cut(s)	670, 1294, 1431
PspN4I	GGNNCC	2 cut(s)	196, 676
PspPI	GGNCC	1 cut(s)	1365
PsuI	RGATCY	1 cut(s)	1298
RsaI	GTAC	4 cut(s)	109, 781, 1347, 1430
RsaNI	GTAC	4 cut(s)	108, 780, 1346, 1429
RseI	CAYNNNNRTG	3 cut(s)	266, 377, 1194
SatI	GCNGC	2 cut(s)	331, 1167
Sau3AI	GATC	3 cut(s)	277, 1210, 1298
Sau96I	GGNCC	1 cut(s)	1365
SchI	GAGTC	1 cut(s)	528
ScrFI	CCNGG	3 cut(s)	672, 1296, 1433
SduI	GDGCHC	5 cut(s)	128, 648, 770, 1089, 1201
SfaNI	GCATC	3 cut(s)	363, 970, 1277
SfcI	CTRYAG	1 cut(s)	384
SinI	GGWCC	1 cut(s)	1365
SmiMI	CAYNNNNRTG	3 cut(s)	266, 377, 1194
SmlI	CTYRAG	2 cut(s)	800, 1202
SmoI	CTYRAG	2 cut(s)	800, 1202
SnaBI	TACGTA	1 cut(s)	1402
SsiI	CCGC	1 cut(s)	116
SspMI	CTAG	5 cut(s)	219, 578, 702, 920, 1284
StyD4I	CCNGG	3 cut(s)	670, 1294, 1431
StyI	CCWWGG	5 cut(s)	349, 358, 426, 903, 1214
TaaI	ACNGT	2 cut(s)	724, 1428
TaiI	ACGT	2 cut(s)	1074, 1404
TaqII	GACCGA	1 cut(s)	1382
TatI	WGTACW	2 cut(s)	779, 1345
TfiI	GAWTC	2 cut(s)	821, 860
TscAI	CASTG	1 cut(s)	1431
TseFI	GTSAC	3 cut(s)	389, 430, 464
TseI	GCWGC	2 cut(s)	330, 1166
Tsp45I	GTSAC	3 cut(s)	389, 430, 464
TspDTI	ATGAA	5 cut(s)	286, 324, 625, 894, 1178
TspRI	CASTG	1 cut(s)	1431
VneI	GTGCAC	1 cut(s)	1085
VpaK11BI	GGWCC	1 cut(s)	1365
XapI	RAATTY	8 cut(s)	612, 772, 1028, 1079, 1112, 1259, 1351, 1406
XbaI	TCTAGA	1 cut(s)	1283
XcmI	CCANNNNNNNNNTGG	1 cut(s)	356
XmnI	GAANNNNTTC	2 cut(s)	233, 293
XspI	CTAG	5 cut(s)	219, 578, 702, 920, 1284

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.