AT3G28720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012436
Physical Location & Seq
Forward (+)
10782095 .. 10784634
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28720.2

Sequence Viewer

Length: 2064 bp
ATGACGCACCACCGCATCACCACCTACTTCAATGGCGGATCCGCCTCCATCTTCCTCCTCCTCCTACTCATCACCACCACTTCCTCTTCTTCTCCACCAATCCCAGGCCTAGACACCTTCCTAACAAACCAATATCGTCTCGACCCAAAATCATCAAACGACTCATTCACATCACTCTCCTCCTCTCTCAAACGATCTCTCTCTTCTTCATCAATCCACTTCTCATCACTCTCCAAATCTCTCCTCTCTCTCTCAATCTCAATCCCACTCAACGTCCGTTTCATCGGAGACTCTTTCCCTTCCTCCGCCGCCTCCACTCTCTCCGAATTCATCTCCGCCGCCGTAACTAACGACAACTTCCATGTAATCTCTCCATCTCCAGATTCATCAACAAATCACAAACTCGTTATCTCTCACTCTCTCCACCTTGACGCTTCTCTATCTCCCCAATCTCTCTCCACCAGACTCGATTCAACTTTGAAAACCCTAATTTCATCAACAACTTCATCTCTCAGATCTAATCTCCTTTCGATCCAGTACAATCCAATCGATGAGATTATAAAGCAAGAGTATGAGAAAGAGAAGCATGGAGATGGTGGTGTTTACATTTACTTGATCTCTTTAGGCTCACAAGCTAAACCTTATGCTTATAGTTACTCTCATGGTGATTCATCAGCTGGGTTTACGAAATGTTTAGGTAGTATTTGGACTGGTAAAGATCGTTATCTATGGATTGATCTCTCTGCTGGTCCTGTAGATTATGGTCCTGCTTTATCTGGTGATGGTGTTTTACCAAGAGGTGAGTTTCATCCTTTAGCTGCTTTACATGGTCGTCCTAAATCAGAAAAAGCTTTACTTGCGGATTTGGCTTCGTTGGTGTATAATGCTTATCAGGTTTTGATTGTTCCTTCTTTGAGAATCCCTGTGTACTTTGAGGATACGTTGGTGGTTCAGTTTATTCATGTGTATGGGTCAGAGGTTAAGGATTCGAGTGGGTTAGATTTGGAGTTTGTGAAGAGAACGTTTATGGATGAAGCTGAGAGTGGTGGGTTGTTGTTAGGTGAACAGAAGTTGAGTTTTAAGAGTTATAGTGTGAATTATAGAGAATGTTCGATTTGTTCGTTTGCGGTATCTCGTGGGATGAATTCGTATACTTCTAGGTTTTTGTTTGATAACTATACTTTGATTGTGAGTGAGTATTTGGATTCTAAGCATATGCATCGGGCGTTGACTGATTCGGCTGAGGAGTTGAGGAGAGTTGCGGGGATTGTTGAAGAAGAAGGAAATGAGTTTGCTAGGGTTTTACCTGTTTATGTGTTTGATTTGGATATCAATACGCCTTTGTTGCTTGATCGGTATCATCAGTCTGTTGCTTTTAGAGATATGGTGATTGCTGTTAGAACTAGAGGAACTCAAACGGTTAGTGATTATACTTGCAACGGGCGTCATGTGTTTGTTCATACGAGAGACTTAGAACGACCTCTCGTTGGTTCCATTCTGCAGAGTATGTGGGGTGTATCTTCTACTCATTTGACATGGAGCCCGAGACATAACACAACTCTAGTTGATTATACATGGAGCATTGGGCAAACACCGTTTGGACCATTCTCTGATATCTCGTCTCTATCGTTTGTTCAGAAAGATGCTGCTAAGAGAAACGTGATTTTGACATCTTTGAACACAACTATCACAAGTGCAATCGATGTTATCGATTCCGCGGTTGCATATGGAGGAGATGTGATCCTACGGAAACAGAATCGCCACTCTGAGTTCATGCAAAGGTGGAATCTTATGCAGTACAAGCTGGATAAAACGGTTTCTGCTCTATCACACAATGAGTTCGAGATGGCTTTGTTCTACTTAAGATCAGCTTCACACGACTTGTACTCAGTGCATTCAGTAGTGTACCTCGCGTCACAACGGGTAGAAGCGAGTCTCAACTGTTTCAAAGATCCACCGTTCCCATGGGGAACTGTTTCAGTGTCGGGATTCGGGTTAATGGCTGTTGGTTACGTGTTCTCGAAAAGAGATAGACTCTTTAGGAACAAACGAAAACAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

687

Amino Acids

76.47

Weight (kDa)

6.91

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 136 - 352 2.3e-84 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AccI GTMKAC 1 cut(s) 1151
AccII CGCG 2 cut(s) 1718, 1913
AclI AACGTT 1 cut(s) 1022
AclWI GGATC 5 cut(s) 33, 46, 526, 1735, 1946
AcsI RAATTY 2 cut(s) 326, 1144
AcyI GRCGYC 1 cut(s) 1444
AfaI GTAC 5 cut(s) 539, 929, 1799, 1886, 1907
AfiI CCNNNNNNNGG 2 cut(s) 104, 1487
AflII CTTAAG 1 cut(s) 1861
AflIII ACRYGT 1 cut(s) 2013
AgsI TTSAA 6 cut(s) 31, 474, 481, 1274, 1678, 1948
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 7 cut(s) 635, 677, 818, 851, 1037, 1804, 1871
AluI AGCT 7 cut(s) 635, 677, 818, 851, 1037, 1804, 1871
Alw26I GTCTC 6 cut(s) 143, 282, 1461, 1540, 1626, 1940
AlwI GGATC 5 cut(s) 33, 46, 526, 1735, 1946
Ama87I CYCGRG 1 cut(s) 1543
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 818, 1646
ApoI RAATTY 2 cut(s) 326, 1144
Asp700I GAANNNNTTC 1 cut(s) 1975
AspS9I GGNCC 3 cut(s) 749, 764, 1601
AsuHPI GGTGA 7 cut(s) 10, 64, 677, 791, 812, 1073, 1399
AvaI CYCGRG 1 cut(s) 1543
AvaII GGWCC 3 cut(s) 749, 764, 1601
BaeI ACNNNNGTAYC 2 cut(s) 930, 963
BamHI GGATCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 1544
BauI CACGAG 1 cut(s) 1134
BbvCI CCTCAGC 1 cut(s) 1242
BbvI GCAGC 2 cut(s) 805, 1633
BccI CCATC 5 cut(s) 56, 382, 587, 776, 1840
BceAI ACGGC 1 cut(s) 326
BcgI CGANNNNNNTGC 2 cut(s) 1202, 1236
BciT130I CCWGG 1 cut(s) 105
BciVI GTATCC 1 cut(s) 931
BcoDI GTCTC 6 cut(s) 143, 282, 1461, 1540, 1626, 1940
BfaI CTAG 5 cut(s) 110, 1158, 1296, 1404, 1562
BfmI CTRYAG 2 cut(s) 753, 1499
BfrI CTTAAG 1 cut(s) 1861
BfuI GTATCC 1 cut(s) 931
BglII AGATCT 1 cut(s) 515
BisI GCNGC 4 cut(s) 309, 339, 819, 1647
BlsI GCNGC 4 cut(s) 310, 340, 820, 1648
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 3 cut(s) 749, 764, 1601
BmeT110I CYCGRG 1 cut(s) 1543
BmgT120I GGNCC 3 cut(s) 749, 764, 1601
BmiI GGNNCC 3 cut(s) 40, 1492, 1541
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 3 cut(s) 24, 1228, 1633
BplI GAGNNNNNCTC 2 cut(s) 2019, 2051
BpmI CTGGAG 1 cut(s) 363
Bpu10I CCTNAGC 1 cut(s) 1242
Bsa29I ATCGAT 3 cut(s) 549, 1701, 1710
BsaAI YACGTR 1 cut(s) 2014
BsaBI GATNNNNATC 2 cut(s) 723, 1356
BsaHI GRCGYC 1 cut(s) 1444
BsaJI CCNNGG 3 cut(s) 103, 1716, 1964
BsaXI ACNNNNNCTCC 2 cut(s) 1722, 1752
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 1487
Bse1I ACTGG 2 cut(s) 535, 715
Bse8I GATNNNNATC 2 cut(s) 723, 1356
BseBI CCWGG 1 cut(s) 105
BseCI ATCGAT 3 cut(s) 549, 1701, 1710
BseDI CCNNGG 3 cut(s) 103, 1716, 1964
BseGI GGATG 3 cut(s) 808, 1036, 1146
BseJI GATNNNNATC 2 cut(s) 723, 1356
BseLI CCNNNNNNNGG 2 cut(s) 104, 1487
BseMII CTCAG 5 cut(s) 526, 1029, 1233, 1758, 1902
BseNI ACTGG 2 cut(s) 535, 715
BseRI GAGGAG 8 cut(s) 47, 50, 169, 172, 233, 1259, 1267, 1746
BseXI GCAGC 2 cut(s) 805, 1633
BseYI CCCAGC 1 cut(s) 677
Bsh1236I CGCG 2 cut(s) 1718, 1913
BshFI GGCC 1 cut(s) 108
BshVI ATCGAT 3 cut(s) 549, 1701, 1710
BsiHKCI CYCGRG 1 cut(s) 1543
BslI CCNNNNNNNGG 2 cut(s) 104, 1487
BsmAI GTCTC 6 cut(s) 143, 282, 1461, 1540, 1626, 1940
BsmBI CGTCTC 2 cut(s) 143, 1626
BsmI GAATGC 1 cut(s) 1894
BsnI GGCC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 1543
Bsp1286I GDGCHC 1 cut(s) 1544
Bsp19I CCATGG 1 cut(s) 1964
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 5 cut(s) 525, 1030, 1234, 1759, 1901
BspDI ATCGAT 3 cut(s) 549, 1701, 1710
BspFNI CGCG 2 cut(s) 1718, 1913
BspLI GGNNCC 3 cut(s) 40, 1492, 1541
BspMAI CTGCAG 1 cut(s) 1503
BspPI GGATC 5 cut(s) 33, 46, 526, 1735, 1946
BspTI CTTAAG 1 cut(s) 1861
BsrI ACTGG 2 cut(s) 535, 715
BssECI CCNNGG 3 cut(s) 103, 1716, 1964
BssNAI GTATAC 1 cut(s) 1152
BssNI GRCGYC 1 cut(s) 1444
BssSI CACGAG 1 cut(s) 1134
BssT1I CCWWGG 1 cut(s) 1964
Bst1107I GTATAC 1 cut(s) 1152
Bst2BI CACGAG 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 7 cut(s) 1420, 1596, 1816, 1943, 1959, 1975, 2058
Bst6I CTCTTC 3 cut(s) 91, 208, 1010
BstACI GRCGYC 1 cut(s) 1444
BstAFI CTTAAG 1 cut(s) 1861
BstBAI YACGTR 1 cut(s) 2014
BstDEI CTNAG 8 cut(s) 512, 1038, 1209, 1242, 1471, 1650, 1767, 1888
BstDSI CCRYGG 2 cut(s) 1716, 1964
BstF5I GGATG 3 cut(s) 808, 1036, 1146
BstFNI CGCG 2 cut(s) 1718, 1913
BstMAI GTCTC 6 cut(s) 143, 282, 1461, 1540, 1626, 1940
BstMWI GCNNNNNNNGC 4 cut(s) 857, 866, 1345, 1801
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 2 cut(s) 753, 1499
BstUI CGCG 2 cut(s) 1718, 1913
BstV1I GCAGC 2 cut(s) 805, 1633
BstX2I RGATCY 3 cut(s) 38, 515, 1951
BstYI RGATCY 3 cut(s) 38, 515, 1951
BstZ17I GTATAC 1 cut(s) 1152
Bsu15I ATCGAT 3 cut(s) 549, 1701, 1710
BsuI GTATCC 1 cut(s) 931
BsuRI GGCC 1 cut(s) 108
BsuTUI ATCGAT 3 cut(s) 549, 1701, 1710
BtgI CCRYGG 2 cut(s) 1716, 1964
BtsCI GGATG 3 cut(s) 808, 1036, 1146
BtsIMutI CAGTG 2 cut(s) 1896, 1986
Cfr13I GGNCC 3 cut(s) 749, 764, 1601
Cfr42I CCGCGG 1 cut(s) 1719
ClaI ATCGAT 3 cut(s) 549, 1701, 1710
CseI GACGC 4 cut(s) 13, 440, 1433, 1902
Csp6I GTAC 5 cut(s) 538, 928, 1798, 1885, 1906
CviQI GTAC 5 cut(s) 538, 928, 1798, 1885, 1906
DdeI CTNAG 8 cut(s) 512, 1038, 1209, 1242, 1471, 1650, 1767, 1888
Eam1104I CTCTTC 3 cut(s) 91, 208, 1010
EarI CTCTTC 3 cut(s) 91, 208, 1010
EciI GGCGGA 4 cut(s) 31, 51, 295, 325
Eco130I CCWWGG 1 cut(s) 1964
Eco147I AGGCCT 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 1544
Eco32I GATATC 2 cut(s) 1330, 1615
Eco47I GGWCC 3 cut(s) 749, 764, 1601
Eco88I CYCGRG 1 cut(s) 1543
EcoRI GAATTC 2 cut(s) 326, 1144
EcoRII CCWGG 1 cut(s) 103
EcoRV GATATC 2 cut(s) 1330, 1615
EcoT14I CCWWGG 1 cut(s) 1964
EcoT22I ATGCAT 1 cut(s) 1221
EcoT38I GRGCYC 1 cut(s) 1544
ErhI CCWWGG 1 cut(s) 1964
Esp3I CGTCTC 2 cut(s) 143, 1626
FalI AAGNNNNNCTT 4 cut(s) 840, 872, 1855, 1887
FauI CCCGC 1 cut(s) 1255
FauNDI CATATG 2 cut(s) 1215, 1726
FblI GTMKAC 1 cut(s) 1151
Fnu4HI GCNGC 4 cut(s) 309, 339, 819, 1647
FokI GGATG 3 cut(s) 795, 1043, 1153
FriOI GRGCYC 1 cut(s) 1544
Fsp4HI GCNGC 4 cut(s) 309, 339, 819, 1647
FspBI CTAG 5 cut(s) 110, 1158, 1296, 1404, 1562
GluI GCNGC 4 cut(s) 309, 339, 819, 1647
GsaI CCCAGC 1 cut(s) 681
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 1 cut(s) 108
HgaI GACGC 4 cut(s) 13, 440, 1433, 1902
Hin1I GRCGYC 1 cut(s) 1444
HincII GTYRAC 1 cut(s) 1230
HindII GTYRAC 1 cut(s) 1230
HindIII AAGCTT 1 cut(s) 849
HphI GGTGA 7 cut(s) 10, 64, 677, 791, 812, 1073, 1399
Hpy166II GTNNAC 7 cut(s) 604, 684, 928, 1064, 1152, 1230, 1906
Hpy188I TCNGA 9 cut(s) 287, 325, 515, 844, 976, 1612, 1638, 1768, 2063
Hpy188III TCNNGA 5 cut(s) 140, 380, 1843, 1986, 2020
Hpy8I GTNNAC 7 cut(s) 604, 684, 928, 1064, 1152, 1230, 1906
HpyAV CCTTC 4 cut(s) 127, 309, 918, 1274
HpyCH4III ACNGT 7 cut(s) 1420, 1596, 1816, 1943, 1959, 1975, 2058
HpyCH4IV ACGT 5 cut(s) 273, 941, 1022, 1659, 2013
HpyCH4V TGCA 8 cut(s) 1219, 1437, 1501, 1697, 1724, 1777, 1795, 1894
HpyF10VI GCNNNNNNNGC 4 cut(s) 857, 866, 1345, 1801
HpyF3I CTNAG 8 cut(s) 512, 1038, 1209, 1242, 1471, 1650, 1767, 1888
HpySE526I ACGT 5 cut(s) 273, 941, 1022, 1659, 2013
Hsp92I GRCGYC 1 cut(s) 1444
KspI CCGCGG 1 cut(s) 1719
LmnI GCTCC 2 cut(s) 1539, 1578
Lsp1109I GCAGC 2 cut(s) 805, 1633
LweI GCATC 3 cut(s) 24, 1228, 1633
MaeI CTAG 5 cut(s) 110, 1158, 1296, 1404, 1562
MaeII ACGT 5 cut(s) 273, 941, 1022, 1659, 2013
MaeIII GTNAC 4 cut(s) 343, 653, 1914, 2009
MboII GAAGA 9 cut(s) 43, 78, 81, 195, 198, 1027, 1286, 1289, 1512
MflI RGATCY 3 cut(s) 38, 515, 1951
MhlI GDGCHC 1 cut(s) 1544
MluCI AATT 4 cut(s) 326, 489, 1096, 1144
MlyI GAGTC 5 cut(s) 155, 284, 459, 1942, 2028
Mph1103I ATGCAT 1 cut(s) 1221
MroXI GAANNNNTTC 1 cut(s) 1975
MseI TTAA 4 cut(s) 981, 1080, 1862, 1997
MslI CAYNNNNRTG 2 cut(s) 591, 966
MspA1I CMGCKG 2 cut(s) 677, 1718
MspCI CTTAAG 1 cut(s) 1861
MspR9I CCNGG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 1894
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 2 cut(s) 1718, 1913
MwoI GCNNNNNNNGC 4 cut(s) 857, 866, 1345, 1801
NcoI CCATGG 1 cut(s) 1964
NdeI CATATG 2 cut(s) 1215, 1726
NlaIV GGNNCC 3 cut(s) 40, 1492, 1541
NmuCI GTSAC 1 cut(s) 1914
NsiI ATGCAT 1 cut(s) 1221
PceI AGGCCT 1 cut(s) 108
PcsI WCGNNNNNNNCGW 3 cut(s) 1118, 1625, 1707
PctI GAATGC 1 cut(s) 1894
PdmI GAANNNNTTC 1 cut(s) 1975
PkrI GCNGC 4 cut(s) 310, 340, 820, 1648
PleI GAGTC 5 cut(s) 155, 284, 459, 1941, 2028
PpsI GAGTC 5 cut(s) 155, 284, 459, 1941, 2028
Ppu21I YACGTR 1 cut(s) 2014
PsiI TTATAA 1 cut(s) 560
Psp1406I AACGTT 1 cut(s) 1022
Psp6I CCWGG 1 cut(s) 103
PspFI CCCAGC 1 cut(s) 677
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 3 cut(s) 40, 1492, 1541
PspPI GGNCC 3 cut(s) 749, 764, 1601
PstI CTGCAG 1 cut(s) 1503
PsuI RGATCY 3 cut(s) 38, 515, 1951
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 5 cut(s) 539, 929, 1799, 1886, 1907
RsaNI GTAC 5 cut(s) 538, 928, 1798, 1885, 1906
RseI CAYNNNNRTG 2 cut(s) 591, 966
SacII CCGCGG 1 cut(s) 1719
SaqAI TTAA 4 cut(s) 981, 1080, 1862, 1997
SatI GCNGC 4 cut(s) 309, 339, 819, 1647
Sau96I GGNCC 3 cut(s) 749, 764, 1601
SchI GAGTC 5 cut(s) 155, 284, 459, 1942, 2028
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 1544
SfaNI GCATC 3 cut(s) 24, 1228, 1633
SfcI CTRYAG 2 cut(s) 753, 1499
Sfr303I CCGCGG 1 cut(s) 1719
SgrBI CCGCGG 1 cut(s) 1719
SinI GGWCC 3 cut(s) 749, 764, 1601
SmiMI CAYNNNNRTG 2 cut(s) 591, 966
SmlI CTYRAG 1 cut(s) 1861
SmoI CTYRAG 1 cut(s) 1861
Sse9I AATT 4 cut(s) 326, 489, 1096, 1144
SseBI AGGCCT 1 cut(s) 108
SspMI CTAG 5 cut(s) 110, 1158, 1296, 1404, 1562
StuI AGGCCT 1 cut(s) 108
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 1964
TaaI ACNGT 7 cut(s) 1420, 1596, 1816, 1943, 1959, 1975, 2058
TaiI ACGT 5 cut(s) 276, 944, 1025, 1662, 2016
TasI AATT 4 cut(s) 326, 489, 1096, 1144
TatI WGTACW 4 cut(s) 537, 927, 1797, 1884
TauI GCSGC 2 cut(s) 311, 341
Tru1I TTAA 4 cut(s) 981, 1080, 1862, 1997
Tru9I TTAA 4 cut(s) 981, 1080, 1862, 1997
TscAI CASTG 2 cut(s) 1896, 1986
TseFI GTSAC 1 cut(s) 1914
TseI GCWGC 2 cut(s) 818, 1646
Tsp45I GTSAC 1 cut(s) 1914
TspGWI ACGGA 2 cut(s) 266, 1762
TspRI CASTG 2 cut(s) 1896, 1986
Vha464I CTTAAG 1 cut(s) 1861
VpaK11BI GGWCC 3 cut(s) 749, 764, 1601
XapI RAATTY 2 cut(s) 326, 1144
XcmI CCANNNNNNNNNTGG 1 cut(s) 1962
XmiI GTMKAC 1 cut(s) 1151
XmnI GAANNNNTTC 1 cut(s) 1975
XspI CTAG 5 cut(s) 110, 1158, 1296, 1404, 1562
Zsp2I ATGCAT 1 cut(s) 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.