RchiOBHm_Chr2g0169061

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0012436
Physical Location & Seq
Forward (+)
83353195 .. 83355707
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53669

Sequence Viewer

Length: 2043 bp
ATGACCACCACCATGTCCTCCGCCACGGCCACCACCACCACCCTCCTACTCCTCCTAACCCTAATCGCCACCTCCACCTCCACCTCAGCCCCAATTCTCGGCCTCGACACCTTCCTCACCCACCAGTCCCGCTACGACCGGCAAGCCTCCAACGACTCCTTCCAGTCTCTCTCCTCCACTCTCCGCCACTCCCTTTCTCTCTCCTCGCCGTCTCTCTCCCACTCTCTCTCCTCCATCCTCTCCCTCACCATCCCTCTCTCTCTCCACGTCCGCCTCGTCGGCCCTTCCTTCCCTTCCTCCTCCGCCACTCTCCTCTCCTCCTTCCTCTCCGCCTCCCAGACCTCCGATCACTTCCACGTCATCACCCCCGCCGACGCCGCGGACTCTCACCGCTTGGCCGTCAAGCACTCCCTCCACCTCGACGTCAGTCACTCCCCCTCCCTCGCCTCTCAGCTCTCCAAAACCCTAGCCTCCGAGCTCGCCAATACCCCCGCCAGTCTCCGATCGCCGCTCGTCTCCGTCGCCTACGATTCAATCGACCGGATCATCAAGCAGGACTTCGACAAAGAGAAGCCGGTCCACGGCGTTTACCTCTATTTCTTGGATTTGGGGCCCCAATCCAAGAGCTATGCTTACAGCTATGGATCTGGTGACTCCTCTCCTGGCTTCACCAAGTGCTTGGGCAGCGTTTGGACCGGGTCGGACCGCTACATTTGGATCGATTTGGGCGCCGGACCGGTCGACTACGGCCCGGCGCTTTCCGGCGACGGTGTCTTGCCGAAAGGCGAGTTTCATCCTCTGGCGGCGTTGCACGGCTCGCCCAAAGCTCAGAAGGCTTTGCTGGCGGACTTGGCTTCCTTGGTTTGGAGTGCTTACCAGGTTCTTCTTGTTCCCTCTTTGAGAATTCCTGTTCAGTATGAGAATTCTTTGATTGTTCAGTTTATACATGTTTATGGAGGTGAGAGTGGTAAGGATTCGAGTGGTTTGGATTGGAAATCGATTGAGAGGACCTTTATGGATGAGGCTAAGGATAATGGCTTGTTGTTAGGGGATCAGTCTTTGAGATTCAAGAATTATAAGGTGAAGTATTCGGAGTGCCCGATATGTTCATTTGCTATTGCCAGGTCGATTAATTCATATACGTCGAGGTTCTTGTTTGATAACTATACGTTGATTGCAAGTGAGTATTTGGATTCGAAGAGATTGCATCAGATATTGTCAGACTCTGCTGATGAGTTTAGGAGAGTGGCAGGGTTTCCTGAGGAGGATTTTGGCAGGGTGCTTCCTGTGTATGTGTTTGATTTGGACTACCACATGCTGTTGCTGCTCGATAGGTATCACCAAACTGTTGCTTTTAGAGATATGGTGATTGCAGTGAGGACCAGGAACACACAGACTGTGAGTGATTATAGCTGCAATGGCCGCCATGTGTTTACACAGACGAGGGAGCTTGAGAGACCACTTGTTGGTTCGATTTTACAGAGTATGTGGGGAGTGTCGCCGACTCATATGTTGTGGAGCCCTAGGCACAATACTACGTTGGTGGATTACACGTGGAGTGTTGGGCAGACTCCATTTGGGCCGTTCTCGGAGATTTCATCACTATCATTCGTACAGAAGGATGCTGCTCGGAGAAATGTTCTTTTGACATCACTGAATTACAGCATATCAAGTGCTGTAGATGTTCTTGAATCCATAGCTGCACATGGTGGGGAGAGAAAGCTCCTGAAACGTAATCGACATGTTGAGTTCGTACAAAGGTGGAATTTCTTCAAGTACAAGCTGGACAAAGCAGTTGCTGCAATGTCACATTTAGACTTTGAGATGGCTTTATACTATATGAGGTCCTCAGATCATGATCTATATGCTATCCACTCTATTGTGTATCATGCCTCCCAGGAATTGGAGGCATCCCTTGTATGTTTCAAGGATCCACCATTTCCGTGGTCTTTTGTGTTTATGGTTGCAGTGCTTCTCCTTCTTGGCTATTATATTAGTTCTAGAAAACACAAACTATTCAGAAGTAAGAGTAAGCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

680

Amino Acids

75.83

Weight (kDa)

7.41

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 85 - 298 1e-87 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1077
AatII GACGTC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 728
AccB7I CCANNNNNTGG 2 cut(s) 1466, 1903
AccBSI CCGCTC 1 cut(s) 511
AccI GTMKAC 1 cut(s) 741
AccII CGCG 1 cut(s) 380
AclWI GGATC 6 cut(s) 551, 652, 725, 1059, 1925, 1938
AcoI YGGCCR 3 cut(s) 27, 396, 1420
AcsI RAATTY 3 cut(s) 903, 922, 1765
AcvI CACGTG 1 cut(s) 1554
AcyI GRCGYC 3 cut(s) 375, 423, 729
AdeI CACNNNGTG 2 cut(s) 675, 1709
AfaI GTAC 3 cut(s) 1614, 1755, 1778
AfiI CCNNNNNNNGG 5 cut(s) 98, 581, 864, 1466, 1903
AflIII ACRYGT 3 cut(s) 946, 1551, 1741
AgeI ACCGGT 1 cut(s) 736
AgsI TTSAA 5 cut(s) 534, 1069, 1691, 1774, 1927
AjiI CACGTC 2 cut(s) 268, 358
AjnI CCWGG 5 cut(s) 661, 876, 1121, 1382, 1896
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 5 cut(s) 171, 216, 503, 520, 1450
AlwI GGATC 6 cut(s) 551, 652, 725, 1059, 1925, 1938
AlwNI CAGNNNCTG 2 cut(s) 1226, 1799
AoxI GGCC 8 cut(s) 27, 100, 280, 396, 611, 748, 1420, 1580
ApaI GGGCCC 1 cut(s) 615
ApeKI GCWGC 6 cut(s) 684, 1324, 1413, 1625, 1700, 1799
ApoI RAATTY 3 cut(s) 903, 922, 1765
AseI ATTAAT 1 cut(s) 1131
AsiGI ACCGGT 1 cut(s) 736
Asp700I GAANNNNTTC 1 cut(s) 1769
AspA2I CCTAGG 1 cut(s) 1523
AspLEI GCGC 2 cut(s) 731, 757
AsuC2I CCSGG 2 cut(s) 697, 752
AsuII TTCGAA 1 cut(s) 1196
AvaII GGWCC 7 cut(s) 577, 693, 703, 734, 1008, 1380, 1845
AvrII CCTAGG 1 cut(s) 1523
AxyI CCTNAGG 1 cut(s) 1260
BaeGI GKGCMC 2 cut(s) 615, 1100
BamHI GGATCC 1 cut(s) 1930
BanI GGYRCC 1 cut(s) 728
BanII GRGCYC 3 cut(s) 480, 615, 1523
BbrPI CACGTG 1 cut(s) 1554
Bbv12I GWGCWC 1 cut(s) 480
BbvCI CCTCAGC 1 cut(s) 85
BbvI GCAGC 6 cut(s) 696, 1311, 1400, 1612, 1687, 1786
BccI CCATC 3 cut(s) 242, 257, 1819
BceAI ACGGC 7 cut(s) 42, 193, 383, 598, 763, 829, 1567
BcgI CGANNNNNNTGC 2 cut(s) 1186, 1220
BciT130I CCWGG 5 cut(s) 663, 878, 1123, 1384, 1898
BcnI CCSGG 2 cut(s) 697, 752
BcoDI GTCTC 5 cut(s) 171, 216, 503, 520, 1450
BfaI CTAG 3 cut(s) 467, 1524, 2001
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 2 cut(s) 732, 758
BglI GCCNNNNNGGC 1 cut(s) 279
BlnI CCTAGG 1 cut(s) 1523
Bme1390I CCNGG 7 cut(s) 663, 697, 752, 878, 1123, 1384, 1898
Bme18I GGWCC 7 cut(s) 577, 693, 703, 734, 1008, 1380, 1845
BmgBI CACGTC 2 cut(s) 268, 358
BmiI GGNNCC 6 cut(s) 612, 613, 614, 730, 1520, 1932
BmrFI CCNGG 7 cut(s) 663, 697, 752, 878, 1123, 1384, 1898
BmsI GCATC 3 cut(s) 1216, 1612, 1919
BoxI GACNNNNGTC 1 cut(s) 426
Bpu10I CCTNAGC 2 cut(s) 85, 1026
Bpu14I TTCGAA 1 cut(s) 1196
BpuEI CTTGAG 1 cut(s) 1472
BpuMI CCSGG 2 cut(s) 697, 752
Bsa29I ATCGAT 2 cut(s) 720, 998
BsaAI YACGTR 1 cut(s) 1554
BsaBI GATNNNNATC 2 cut(s) 1335, 1857
BsaHI GRCGYC 3 cut(s) 375, 423, 729
BsaI GGTCTC 1 cut(s) 1450
BsaJI CCNNGG 7 cut(s) 24, 378, 580, 858, 1523, 1896, 1943
BsaWI WCCGGW 2 cut(s) 540, 736
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 422, 452
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 581, 864, 1466, 1903
Bse118I RCCGGY 3 cut(s) 138, 574, 736
Bse1I ACTGG 3 cut(s) 124, 163, 495
Bse21I CCTNAGG 1 cut(s) 1260
Bse3DI GCAATG 2 cut(s) 1423, 1809
Bse8I GATNNNNATC 2 cut(s) 1335, 1857
BseBI CCWGG 5 cut(s) 663, 878, 1123, 1384, 1898
BseCI ATCGAT 2 cut(s) 720, 998
BseDI CCNNGG 7 cut(s) 24, 378, 580, 858, 1523, 1896, 1943
BseGI GGATG 6 cut(s) 234, 249, 793, 1024, 1627, 1910
BseJI GATNNNNATC 2 cut(s) 1335, 1857
BseLI CCNNNNNNNGG 5 cut(s) 98, 581, 864, 1466, 1903
BseMI GCAATG 2 cut(s) 1423, 1809
BseMII CTCAG 5 cut(s) 99, 464, 842, 1251, 1863
BseNI ACTGG 3 cut(s) 124, 163, 495
BseRI GAGGAG 9 cut(s) 41, 163, 193, 220, 289, 302, 307, 646, 1277
BseSI GKGCMC 2 cut(s) 615, 1100
BseXI GCAGC 6 cut(s) 696, 1311, 1400, 1612, 1687, 1786
BsgI GTGCAG 1 cut(s) 1686
Bsh1236I CGCG 1 cut(s) 380
Bsh1285I CGRYCG 4 cut(s) 139, 506, 541, 741
BshFI GGCC 8 cut(s) 29, 102, 282, 398, 613, 750, 1422, 1582
BshNI GGYRCC 1 cut(s) 728
BshTI ACCGGT 1 cut(s) 736
BshVI ATCGAT 2 cut(s) 720, 998
BsiEI CGRYCG 4 cut(s) 139, 506, 541, 741
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 8 cut(s) 139, 541, 575, 696, 732, 737, 752, 762
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 5 cut(s) 98, 581, 864, 1466, 1903
BsmAI GTCTC 5 cut(s) 171, 216, 503, 520, 1450
BsmBI CGTCTC 2 cut(s) 216, 520
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 8 cut(s) 29, 102, 282, 398, 613, 750, 1422, 1582
Bso31I GGTCTC 1 cut(s) 1450
Bsp119I TTCGAA 1 cut(s) 1196
Bsp120I GGGCCC 1 cut(s) 611
Bsp1286I GDGCHC 4 cut(s) 480, 615, 1100, 1523
Bsp143I GATC 9 cut(s) 346, 503, 543, 644, 717, 1051, 1852, 1858, 1930
BspANI GGCC 8 cut(s) 29, 102, 282, 398, 613, 750, 1422, 1582
BspCNI CTCAG 5 cut(s) 98, 463, 841, 1252, 1862
BspDI ATCGAT 2 cut(s) 720, 998
BspFNI CGCG 1 cut(s) 380
BspHI TCATGA 1 cut(s) 1855
BspLI GGNNCC 6 cut(s) 612, 613, 614, 730, 1520, 1932
BspPI GGATC 6 cut(s) 551, 652, 725, 1059, 1925, 1938
BspT104I TTCGAA 1 cut(s) 1196
BspT107I GGYRCC 1 cut(s) 728
BspTNI GGTCTC 1 cut(s) 1450
BsrBI CCGCTC 1 cut(s) 511
BsrDI GCAATG 2 cut(s) 1423, 1809
BsrFI RCCGGY 3 cut(s) 138, 574, 736
BsrI ACTGG 3 cut(s) 124, 163, 495
BssAI RCCGGY 3 cut(s) 138, 574, 736
BssECI CCNNGG 7 cut(s) 24, 378, 580, 858, 1523, 1896, 1943
BssMI GATC 9 cut(s) 346, 503, 543, 644, 717, 1051, 1852, 1858, 1930
BssNI GRCGYC 3 cut(s) 375, 423, 729
BssT1I CCWWGG 2 cut(s) 858, 1523
Bst2UI CCWGG 5 cut(s) 663, 878, 1123, 1384, 1898
Bst4CI ACNGT 3 cut(s) 770, 1348, 1399
Bst6I CTCTTC 1 cut(s) 1193
BstACI GRCGYC 3 cut(s) 375, 423, 729
BstAPI GCANNNNNTGC 1 cut(s) 1799
BstBAI YACGTR 1 cut(s) 1554
BstBI TTCGAA 1 cut(s) 1196
BstC8I GCNNGC 4 cut(s) 144, 480, 818, 843
BstDEI CTNAG 6 cut(s) 85, 450, 828, 1026, 1260, 1849
BstDSI CCRYGG 4 cut(s) 24, 378, 580, 1943
BstF5I GGATG 6 cut(s) 234, 249, 793, 1024, 1627, 1910
BstFNI CGCG 1 cut(s) 380
BstH2I RGCGCY 2 cut(s) 732, 758
BstHHI GCGC 2 cut(s) 731, 757
BstKTI GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
BstMAI GTCTC 5 cut(s) 171, 216, 503, 520, 1450
BstMBI GATC 9 cut(s) 346, 503, 543, 644, 717, 1051, 1852, 1858, 1930
BstMCI CGRYCG 4 cut(s) 139, 506, 541, 741
BstNI CCWGG 5 cut(s) 663, 878, 1123, 1384, 1898
BstNSI RCATGY 3 cut(s) 950, 1318, 1745
BstPAI GACNNNNGTC 1 cut(s) 426
BstSCI CCNGG 7 cut(s) 661, 695, 750, 876, 1121, 1382, 1896
BstSFI CTRYAG 1 cut(s) 1677
BstSLI GKGCMC 2 cut(s) 615, 1100
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 6 cut(s) 696, 1311, 1400, 1612, 1687, 1786
BstX2I RGATCY 2 cut(s) 644, 1930
BstXI CCANNNNNNTGG 2 cut(s) 679, 1944
BstYI RGATCY 2 cut(s) 644, 1930
Bsu15I ATCGAT 2 cut(s) 720, 998
Bsu36I CCTNAGG 1 cut(s) 1260
BsuRI GGCC 8 cut(s) 29, 102, 282, 398, 613, 750, 1422, 1582
BsuTUI ATCGAT 2 cut(s) 720, 998
BtgI CCRYGG 4 cut(s) 24, 378, 580, 1943
BtrI CACGTC 2 cut(s) 268, 358
BtsCI GGATG 6 cut(s) 234, 249, 793, 1024, 1627, 1910
BtsI GCAGTG 2 cut(s) 1380, 1974
BtsIMutI CAGTG 3 cut(s) 1380, 1652, 1974
Cac8I GCNNGC 4 cut(s) 144, 480, 818, 843
CaiI CAGNNNCTG 2 cut(s) 1226, 1799
CciI TCATGA 1 cut(s) 1855
CfoI GCGC 2 cut(s) 731, 757
Cfr10I RCCGGY 3 cut(s) 138, 574, 736
Cfr42I CCGCGG 1 cut(s) 381
ClaI ATCGAT 2 cut(s) 720, 998
CpoI CGGWCCG 2 cut(s) 703, 734
CseI GACGC 1 cut(s) 383
CsiI ACCWGGT 1 cut(s) 876
Csp6I GTAC 3 cut(s) 1613, 1754, 1777
CspAI ACCGGT 1 cut(s) 736
CspI CGGWCCG 2 cut(s) 703, 734
CviAII CATG 8 cut(s) 13, 947, 1315, 1427, 1706, 1742, 1856, 1889
CviQI GTAC 3 cut(s) 1613, 1754, 1777
DdeI CTNAG 6 cut(s) 85, 450, 828, 1026, 1260, 1849
DinI GGCGCC 1 cut(s) 730
DpnI GATC 9 cut(s) 348, 505, 545, 646, 719, 1053, 1854, 1860, 1932
DpnII GATC 9 cut(s) 346, 503, 543, 644, 717, 1051, 1852, 1858, 1930
DraIII CACNNNGTG 2 cut(s) 675, 1709
EaeI YGGCCR 3 cut(s) 27, 396, 1420
Eam1104I CTCTTC 1 cut(s) 1193
EarI CTCTTC 1 cut(s) 1193
EciI GGCGGA 6 cut(s) 10, 173, 260, 292, 319, 860
Ecl136II GAGCTC 1 cut(s) 478
Eco130I CCWWGG 2 cut(s) 858, 1523
Eco24I GRGCYC 3 cut(s) 480, 615, 1523
Eco31I GGTCTC 1 cut(s) 1450
Eco47I GGWCC 7 cut(s) 577, 693, 703, 734, 1008, 1380, 1845
Eco53kI GAGCTC 1 cut(s) 478
Eco72I CACGTG 1 cut(s) 1554
Eco81I CCTNAGG 1 cut(s) 1260
EcoICRI GAGCTC 1 cut(s) 478
EcoO109I RGGNCCY 4 cut(s) 611, 612, 1008, 1845
EcoRI GAATTC 2 cut(s) 903, 922
EcoRII CCWGG 5 cut(s) 661, 876, 1121, 1382, 1896
EcoT14I CCWWGG 2 cut(s) 858, 1523
EcoT38I GRGCYC 3 cut(s) 480, 615, 1523
EgeI GGCGCC 1 cut(s) 730
EheI GGCGCC 1 cut(s) 730
ErhI CCWWGG 2 cut(s) 858, 1523
Esp3I CGTCTC 2 cut(s) 216, 520
FaeI CATG 8 cut(s) 16, 950, 1318, 1430, 1709, 1745, 1859, 1892
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 1 cut(s) 112
FatI CATG 8 cut(s) 12, 946, 1314, 1426, 1705, 1741, 1855, 1888
FauI CCCGC 3 cut(s) 137, 376, 499
FauNDI CATATG 1 cut(s) 1509
FblI GTMKAC 1 cut(s) 741
FokI GGATG 6 cut(s) 221, 236, 780, 1031, 1634, 1897
FriOI GRGCYC 3 cut(s) 480, 615, 1523
FspBI CTAG 3 cut(s) 467, 1524, 2001
GlaI GCGC 2 cut(s) 730, 756
HaeII RGCGCY 2 cut(s) 732, 758
HaeIII GGCC 8 cut(s) 29, 102, 282, 398, 613, 750, 1422, 1582
HapII CCGG 8 cut(s) 139, 541, 575, 696, 732, 737, 752, 762
HgaI GACGC 1 cut(s) 383
HhaI GCGC 2 cut(s) 731, 757
Hin1I GRCGYC 3 cut(s) 375, 423, 729
Hin1II CATG 8 cut(s) 16, 950, 1318, 1430, 1709, 1745, 1859, 1892
Hin6I GCGC 2 cut(s) 729, 755
HinP1I GCGC 2 cut(s) 729, 755
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HpaII CCGG 8 cut(s) 139, 541, 575, 696, 732, 737, 752, 762
Hpy166II GTNNAC 4 cut(s) 580, 589, 742, 1434
Hpy188III TCNNGA 6 cut(s) 1069, 1259, 1688, 1726, 1856, 2001
Hpy8I GTNNAC 4 cut(s) 580, 589, 742, 1434
Hpy99I CGWCG 6 cut(s) 281, 377, 425, 524, 770, 1147
HpyAV CCTTC 9 cut(s) 121, 169, 294, 298, 303, 331, 826, 1612, 1988
HpyCH4III ACNGT 3 cut(s) 770, 1348, 1399
HpyCH4IV ACGT 8 cut(s) 267, 357, 423, 1142, 1169, 1538, 1553, 1732
HpyCH4V TGCA 8 cut(s) 811, 1178, 1207, 1373, 1416, 1703, 1802, 1967
HpyF3I CTNAG 6 cut(s) 85, 450, 828, 1026, 1260, 1849
HpySE526I ACGT 8 cut(s) 267, 357, 423, 1142, 1169, 1538, 1553, 1732
Hsp92I GRCGYC 3 cut(s) 375, 423, 729
Hsp92II CATG 8 cut(s) 16, 950, 1318, 1430, 1709, 1745, 1859, 1892
HspAI GCGC 2 cut(s) 729, 755
KasI GGCGCC 1 cut(s) 728
KspI CCGCGG 1 cut(s) 381
Kzo9I GATC 9 cut(s) 346, 503, 543, 644, 717, 1051, 1852, 1858, 1930
LmnI GCTCC 3 cut(s) 1447, 1518, 1728
Lsp1109I GCAGC 6 cut(s) 696, 1311, 1400, 1612, 1687, 1786
LweI GCATC 3 cut(s) 1216, 1612, 1919
MabI ACCWGGT 1 cut(s) 876
MaeI CTAG 3 cut(s) 467, 1524, 2001
MaeII ACGT 8 cut(s) 267, 357, 423, 1142, 1169, 1538, 1553, 1732
MaeIII GTNAC 3 cut(s) 428, 650, 1806
MalI GATC 9 cut(s) 348, 505, 545, 646, 719, 1053, 1854, 1860, 1932
MbiI CCGCTC 1 cut(s) 511
MboI GATC 9 cut(s) 346, 503, 543, 644, 717, 1051, 1852, 1858, 1930
MboII GAAGA 3 cut(s) 875, 1210, 1762
MflI RGATCY 2 cut(s) 644, 1930
MhlI GDGCHC 4 cut(s) 480, 615, 1100, 1523
MluCI AATT 9 cut(s) 93, 903, 922, 1072, 1132, 1657, 1765, 1901, 2036
Mly113I GGCGCC 1 cut(s) 729
MlyI GAGTC 6 cut(s) 149, 377, 647, 1217, 1498, 1564
MmeI TCCRAC 2 cut(s) 174, 681
MroXI GAANNNNTTC 1 cut(s) 1769
MseI TTAA 1 cut(s) 1131
MslI CAYNNNNRTG 3 cut(s) 11, 951, 1942
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 8 cut(s) 139, 541, 575, 696, 732, 737, 752, 762
MspR9I CCNGG 7 cut(s) 663, 697, 752, 878, 1123, 1384, 1898
MvaI CCWGG 5 cut(s) 663, 878, 1123, 1384, 1898
MvnI CGCG 1 cut(s) 380
NarI GGCGCC 1 cut(s) 729
NciI CCSGG 2 cut(s) 697, 752
NdeI CATATG 1 cut(s) 1509
NdeII GATC 9 cut(s) 346, 503, 543, 644, 717, 1051, 1852, 1858, 1930
NlaIII CATG 8 cut(s) 16, 950, 1318, 1430, 1709, 1745, 1859, 1892
NlaIV GGNNCC 6 cut(s) 612, 613, 614, 730, 1520, 1932
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 3 cut(s) 428, 650, 1806
NspI RCATGY 3 cut(s) 950, 1318, 1745
NspV TTCGAA 1 cut(s) 1196
PagI TCATGA 1 cut(s) 1855
PciI ACATGT 2 cut(s) 946, 1741
PcsI WCGNNNNNNNCGW 3 cut(s) 273, 534, 1097
PdmI GAANNNNTTC 1 cut(s) 1769
PfeI GAWTC 5 cut(s) 530, 974, 1065, 1193, 1691
PflFI GACNNNGTC 2 cut(s) 697, 770
PflMI CCANNNNNTGG 2 cut(s) 1466, 1903
PinAI ACCGGT 1 cut(s) 736
Ple19I CGATCG 1 cut(s) 506
PleI GAGTC 6 cut(s) 149, 377, 647, 1217, 1498, 1564
PluTI GGCGCC 1 cut(s) 732
PmaCI CACGTG 1 cut(s) 1554
PmlI CACGTG 1 cut(s) 1554
PpsI GAGTC 6 cut(s) 149, 377, 647, 1217, 1498, 1564
Ppu21I YACGTR 1 cut(s) 1554
PpuMI RGGWCCY 2 cut(s) 1008, 1845
PscI ACATGT 2 cut(s) 946, 1741
PshAI GACNNNNGTC 1 cut(s) 426
PshBI ATTAAT 1 cut(s) 1131
PsiI TTATAA 1 cut(s) 1077
Psp124BI GAGCTC 1 cut(s) 480
Psp5II RGGWCCY 2 cut(s) 1008, 1845
Psp6I CCWGG 5 cut(s) 661, 876, 1121, 1382, 1896
PspCI CACGTG 1 cut(s) 1554
PspGI CCWGG 5 cut(s) 661, 876, 1121, 1382, 1896
PspN4I GGNNCC 6 cut(s) 612, 613, 614, 730, 1520, 1932
PspOMI GGGCCC 1 cut(s) 611
PspPPI RGGWCCY 2 cut(s) 1008, 1845
PstNI CAGNNNCTG 2 cut(s) 1226, 1799
PsuI RGATCY 2 cut(s) 644, 1930
PsyI GACNNNGTC 2 cut(s) 697, 770
PvuI CGATCG 1 cut(s) 506
RsaI GTAC 3 cut(s) 1614, 1755, 1778
RsaNI GTAC 3 cut(s) 1613, 1754, 1777
RseI CAYNNNNRTG 3 cut(s) 11, 951, 1942
Rsr2I CGGWCCG 2 cut(s) 703, 734
RsrII CGGWCCG 2 cut(s) 703, 734
SacI GAGCTC 1 cut(s) 480
SacII CCGCGG 1 cut(s) 381
SalI GTCGAC 1 cut(s) 740
SaqAI TTAA 1 cut(s) 1131
Sau3AI GATC 9 cut(s) 346, 503, 543, 644, 717, 1051, 1852, 1858, 1930
SchI GAGTC 6 cut(s) 149, 377, 647, 1217, 1498, 1564
ScrFI CCNGG 7 cut(s) 663, 697, 752, 878, 1123, 1384, 1898
SduI GDGCHC 4 cut(s) 480, 615, 1100, 1523
SexAI ACCWGGT 1 cut(s) 876
SfaNI GCATC 3 cut(s) 1216, 1612, 1919
SfcI CTRYAG 1 cut(s) 1677
SfoI GGCGCC 1 cut(s) 730
Sfr303I CCGCGG 1 cut(s) 381
SfuI TTCGAA 1 cut(s) 1196
SgrBI CCGCGG 1 cut(s) 381
SinI GGWCC 7 cut(s) 577, 693, 703, 734, 1008, 1380, 1845
SmiMI CAYNNNNRTG 3 cut(s) 11, 951, 1942
SmlI CTYRAG 1 cut(s) 1451
SmoI CTYRAG 1 cut(s) 1451
Sse9I AATT 9 cut(s) 93, 903, 922, 1072, 1132, 1657, 1765, 1901, 2036
SspDI GGCGCC 1 cut(s) 728
SspMI CTAG 3 cut(s) 467, 1524, 2001
SstI GAGCTC 1 cut(s) 480
StyD4I CCNGG 7 cut(s) 661, 695, 750, 876, 1121, 1382, 1896
StyI CCWWGG 2 cut(s) 858, 1523
TaaI ACNGT 3 cut(s) 770, 1348, 1399
TaiI ACGT 8 cut(s) 270, 360, 426, 1145, 1172, 1541, 1556, 1735
TasI AATT 9 cut(s) 93, 903, 922, 1072, 1132, 1657, 1765, 1901, 2036
TatI WGTACW 1 cut(s) 1776
TauI GCSGC 4 cut(s) 380, 511, 806, 1425
TfiI GAWTC 5 cut(s) 530, 974, 1065, 1193, 1691
Tru1I TTAA 1 cut(s) 1131
Tru9I TTAA 1 cut(s) 1131
TscAI CASTG 3 cut(s) 1380, 1659, 1974
TseFI GTSAC 3 cut(s) 428, 650, 1806
TseI GCWGC 6 cut(s) 684, 1324, 1413, 1625, 1700, 1799
Tsp45I GTSAC 3 cut(s) 428, 650, 1806
TspDTI ATGAA 4 cut(s) 782, 1098, 1125, 1587
TspGWI ACGGA 2 cut(s) 508, 1932
TspRI CASTG 3 cut(s) 1380, 1659, 1974
Tth111I GACNNNGTC 2 cut(s) 697, 770
Van91I CCANNNNNTGG 2 cut(s) 1466, 1903
VpaK11BI GGWCC 7 cut(s) 577, 693, 703, 734, 1008, 1380, 1845
VspI ATTAAT 1 cut(s) 1131
XapI RAATTY 3 cut(s) 903, 922, 1765
XbaI TCTAGA 1 cut(s) 2000
XceI RCATGY 3 cut(s) 950, 1318, 1745
XcmI CCANNNNNNNNNTGG 1 cut(s) 1941
XmaJI CCTAGG 1 cut(s) 1523
XmiI GTMKAC 1 cut(s) 741
XmnI GAANNNNTTC 1 cut(s) 1769
XspI CTAG 3 cut(s) 467, 1524, 2001
ZraI GACGTC 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.