Rw2G050440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012436
Physical Location & Seq
Forward (+)
78069338 .. 78071788
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050440.1

Sequence Viewer

Length: 2049 bp
ATGACCACCACCATGTCCTCCGCCACGGCCACGGTCACCACCCTCCTCCTCCTCCTAACCCTAATCGCCACCTCCACCTCCACCTCCACCTCAGCCCCAATTATCGGCCTCGACACCTTCCTCACCCACCAGTCCCGCTACGACCGGCAAGCCTCCAACGACTCCTTCCAGTCTCTCTCCTCCACTCTCCGCCACTCCCTTTCTCTCTCCTCGCCGTCTCTCTCCCACTCTCTCTCCTCCATCCTCTCCCTCACCATCCCTCTCTCTCTCCACGTCCGCCTCGTCGGCCCTTCCTTCCCTTCCTCCTCCGCCACTCTCCTCTCCTCCTTCCTCTCCGCCTCCCAGACCTCCGATCACTTCCACGTCATCACCCCCGCCGACGCCGCCGACTCTCACCGCTTGGCCGTCAAGCACTCCCTCCACCTCGACGTCAGTCACTCCCCCTCCCTCGCCTCTCAGCTCTCCAAAACCCTAGCCTCCGAGCTCGCCAATACCCCCGCCAGTCTCCGATCGCCGCTCGTCTCCGTCGCCTACGACTCAATCGACCGGATCATCAAGCAGGACTTCGAGAAAGAGAAGCCGGTCCACGGCGTTTACCTCTATTTCTTGGATTTGGGGCCCCAATCCAAGAGCTATGCTTACAGCTATGGATCTGGTGACTCCTCTCCTGGCTTCACCAAGTGCTTGGGCAGCGTTTGGACCGGGTCGGACCGCTACATTTGGATCGATTTGGGCGCCGGACCGGTCGACTACGGCCCGGCGCTTTCCGGCGACGGTGTCTTGCCGAAAGGCGAGTTTCATCCTCTGGCGGCGTTGCACGGCTCGCCCAAAGCTCAGAAGGCTTTGCTGGCGGACTTGGCTTCCTTGGTTTGGAGTGCTTACCAGGTTCTTCTTGTTCCCTCTTTGAGAATTCCTGTTCAGTATGAGAATTCTTTGATTGTTCAGTTTATACATGTTTATGGAGGTGAGAGTGGTAAGGATTCGAGTGGTTTGGATTGGAAATCAATTGAGAGGACCTTTATGGATGAGGCTAAGGATAATGGCTTGTTGGTAGGGGATCAGTCTTTGAGTTTCAAGAATTATAAGGTGAAGTATTCGGAGTGCCCGATATGTTCATTTGCTATTGCCAGGTCGATTAATTCATATACGTCGAGGTTCTTGTTTGATAACTATACGTTGATTGCAAGTGAGTATTTGGATTCGAAGAGATTGCATCAGATATTGTCAGACTCTGCTGATGAGTTTAGGAGAGTGGCAGGGTTTCCTGAGGAGGATTTTGGCAGGGTGCTTCCTGTGTATGTGTTTGATTTGGACTATCACATGCTGTTGCTGCTCGATAGGTATCACCAAACTGTTGCTTTTAGAGATATGGTGATTGCAGTGAGGACCAGGAACACACAGACTGTGAGTGATTATAGCTGCAATGGCCGCCATGTGTTTACACAGACGAGGGAGCTTGAGAGACCACTTGTTGGTTCGATTTTACAGAGTATGTGGGGAGTGTCGCCGACTCATATGTTGTGGAGCCCTAGGCACAATACTACGTTGGTGGATTACACGTGGAGTGTTGGGCAGACTCCATTTGGGCCGTTCTCGGAGATTTCATCACTATCATTCGTACAGAAGGATGCTGCTCGGAGAAATGTTCTTTTGACATCACTAAATTACAGCATATCAAGTGCTGTAGATGTTCTTGAATCCATAGCTGCACATGGTGGGGAGAGAAAGCTCCTGAAACGTAATCGACATGTTGAGTTCGTACAAAGGTGGAATTTCTTCAAGTACAAGCTGGACAAAGCAGTTTCTGCAATGTCACATTTGGACTTTGAGCTGGCTTTATACTATATGAGGTCCTCAGATCATGATCTATATGCTATCCACTCTATTGTGTATCATGCCTCCCAGGAATTGGAGGCATCCCTTGTATGTTTCAAGGATCCACCATTTCCGTGGTCTTTTGTGTTTATGGTTGCAGTGCTTCTCCTTCTTGGCTATTATATTAGTTCTAGAAAACACAAACTATTCAGAAGTAAGAGTAAGCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

682

Amino Acids

75.95

Weight (kDa)

7.16

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 87 - 300 5.3e-88 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1083
AatII GACGTC 1 cut(s) 432
AccB1I GGYRCC 1 cut(s) 734
AccB7I CCANNNNNTGG 2 cut(s) 1472, 1909
AccBSI CCGCTC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 747
AclWI GGATC 6 cut(s) 557, 658, 731, 1065, 1931, 1944
AcoI YGGCCR 3 cut(s) 27, 402, 1426
AcsI RAATTY 3 cut(s) 909, 928, 1771
AcvI CACGTG 1 cut(s) 1560
AcyI GRCGYC 3 cut(s) 381, 429, 735
AdeI CACNNNGTG 2 cut(s) 681, 1715
AfaI GTAC 3 cut(s) 1620, 1761, 1784
AfiI CCNNNNNNNGG 5 cut(s) 104, 587, 870, 1472, 1909
AflIII ACRYGT 3 cut(s) 952, 1557, 1747
AgeI ACCGGT 1 cut(s) 742
AgsI TTSAA 4 cut(s) 1075, 1697, 1780, 1933
AjiI CACGTC 2 cut(s) 274, 364
AjnI CCWGG 5 cut(s) 667, 882, 1127, 1388, 1902
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 5 cut(s) 177, 222, 509, 526, 1456
AlwI GGATC 6 cut(s) 557, 658, 731, 1065, 1931, 1944
AlwNI CAGNNNCTG 2 cut(s) 1232, 1805
AoxI GGCC 8 cut(s) 27, 106, 286, 402, 617, 754, 1426, 1586
ApaI GGGCCC 1 cut(s) 621
ApeKI GCWGC 5 cut(s) 690, 1330, 1419, 1631, 1706
ApoI RAATTY 3 cut(s) 909, 928, 1771
AseI ATTAAT 1 cut(s) 1137
AsiGI ACCGGT 1 cut(s) 742
Asp700I GAANNNNTTC 1 cut(s) 1775
AspA2I CCTAGG 1 cut(s) 1529
AspLEI GCGC 2 cut(s) 737, 763
AsuC2I CCSGG 2 cut(s) 703, 758
AsuII TTCGAA 1 cut(s) 1202
AvaII GGWCC 7 cut(s) 583, 699, 709, 740, 1014, 1386, 1851
AvrII CCTAGG 1 cut(s) 1529
AxyI CCTNAGG 1 cut(s) 1266
BaeGI GKGCMC 2 cut(s) 621, 1106
BamHI GGATCC 1 cut(s) 1936
BanI GGYRCC 1 cut(s) 734
BanII GRGCYC 3 cut(s) 486, 621, 1529
BbrPI CACGTG 1 cut(s) 1560
Bbv12I GWGCWC 1 cut(s) 486
BbvCI CCTCAGC 1 cut(s) 91
BbvI GCAGC 5 cut(s) 702, 1317, 1406, 1618, 1693
BccI CCATC 2 cut(s) 248, 263
BceAI ACGGC 7 cut(s) 42, 199, 389, 604, 769, 835, 1573
BcgI CGANNNNNNTGC 2 cut(s) 1192, 1226
BciT130I CCWGG 5 cut(s) 669, 884, 1129, 1390, 1904
BcnI CCSGG 2 cut(s) 703, 758
BcoDI GTCTC 5 cut(s) 177, 222, 509, 526, 1456
BfaI CTAG 3 cut(s) 473, 1530, 2007
BfmI CTRYAG 1 cut(s) 1683
BfoI RGCGCY 2 cut(s) 738, 764
BglI GCCNNNNNGGC 1 cut(s) 285
BisI GCNGC 9 cut(s) 384, 515, 691, 810, 1331, 1420, 1429, 1632, 1707
BlnI CCTAGG 1 cut(s) 1529
BlsI GCNGC 9 cut(s) 385, 516, 692, 811, 1332, 1421, 1430, 1633, 1708
Bme1390I CCNGG 7 cut(s) 669, 703, 758, 884, 1129, 1390, 1904
Bme18I GGWCC 7 cut(s) 583, 699, 709, 740, 1014, 1386, 1851
BmgBI CACGTC 2 cut(s) 274, 364
BmiI GGNNCC 6 cut(s) 618, 619, 620, 736, 1526, 1938
BmrFI CCNGG 7 cut(s) 669, 703, 758, 884, 1129, 1390, 1904
BmsI GCATC 3 cut(s) 1222, 1618, 1925
BoxI GACNNNNGTC 1 cut(s) 432
Bpu10I CCTNAGC 2 cut(s) 91, 1032
Bpu14I TTCGAA 1 cut(s) 1202
BpuEI CTTGAG 1 cut(s) 1478
BpuMI CCSGG 2 cut(s) 703, 758
Bsa29I ATCGAT 1 cut(s) 726
BsaAI YACGTR 1 cut(s) 1560
BsaBI GATNNNNATC 2 cut(s) 1341, 1863
BsaHI GRCGYC 3 cut(s) 381, 429, 735
BsaI GGTCTC 1 cut(s) 1456
BsaJI CCNNGG 7 cut(s) 24, 30, 586, 864, 1529, 1902, 1949
BsaWI WCCGGW 2 cut(s) 546, 742
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 428, 458
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 587, 870, 1472, 1909
Bse118I RCCGGY 3 cut(s) 144, 580, 742
Bse1I ACTGG 3 cut(s) 130, 169, 501
Bse21I CCTNAGG 1 cut(s) 1266
Bse3DI GCAATG 2 cut(s) 1429, 1815
Bse8I GATNNNNATC 2 cut(s) 1341, 1863
BseBI CCWGG 5 cut(s) 669, 884, 1129, 1390, 1904
BseCI ATCGAT 1 cut(s) 726
BseDI CCNNGG 7 cut(s) 24, 30, 586, 864, 1529, 1902, 1949
BseGI GGATG 6 cut(s) 240, 255, 799, 1030, 1633, 1916
BseJI GATNNNNATC 2 cut(s) 1341, 1863
BseLI CCNNNNNNNGG 5 cut(s) 104, 587, 870, 1472, 1909
BseMI GCAATG 2 cut(s) 1429, 1815
BseMII CTCAG 5 cut(s) 105, 470, 848, 1257, 1869
BseNI ACTGG 3 cut(s) 130, 169, 501
BseSI GKGCMC 2 cut(s) 621, 1106
BseXI GCAGC 5 cut(s) 702, 1317, 1406, 1618, 1693
BsgI GTGCAG 1 cut(s) 1692
Bsh1285I CGRYCG 4 cut(s) 145, 512, 547, 747
BshFI GGCC 8 cut(s) 29, 108, 288, 404, 619, 756, 1428, 1588
BshNI GGYRCC 1 cut(s) 734
BshTI ACCGGT 1 cut(s) 742
BshVI ATCGAT 1 cut(s) 726
BsiEI CGRYCG 4 cut(s) 145, 512, 547, 747
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 8 cut(s) 145, 547, 581, 702, 738, 743, 758, 768
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 5 cut(s) 104, 587, 870, 1472, 1909
BsmAI GTCTC 5 cut(s) 177, 222, 509, 526, 1456
BsmBI CGTCTC 2 cut(s) 222, 526
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 8 cut(s) 29, 108, 288, 404, 619, 756, 1428, 1588
Bso31I GGTCTC 1 cut(s) 1456
Bsp119I TTCGAA 1 cut(s) 1202
Bsp120I GGGCCC 1 cut(s) 617
Bsp1286I GDGCHC 4 cut(s) 486, 621, 1106, 1529
Bsp143I GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
BspANI GGCC 8 cut(s) 29, 108, 288, 404, 619, 756, 1428, 1588
BspCNI CTCAG 5 cut(s) 104, 469, 847, 1258, 1868
BspDI ATCGAT 1 cut(s) 726
BspHI TCATGA 1 cut(s) 1861
BspLI GGNNCC 6 cut(s) 618, 619, 620, 736, 1526, 1938
BspPI GGATC 6 cut(s) 557, 658, 731, 1065, 1931, 1944
BspT104I TTCGAA 1 cut(s) 1202
BspT107I GGYRCC 1 cut(s) 734
BspTNI GGTCTC 1 cut(s) 1456
BsrBI CCGCTC 1 cut(s) 517
BsrDI GCAATG 2 cut(s) 1429, 1815
BsrFI RCCGGY 3 cut(s) 144, 580, 742
BsrI ACTGG 3 cut(s) 130, 169, 501
BssAI RCCGGY 3 cut(s) 144, 580, 742
BssECI CCNNGG 7 cut(s) 24, 30, 586, 864, 1529, 1902, 1949
BssMI GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
BssNI GRCGYC 3 cut(s) 381, 429, 735
BssT1I CCWWGG 2 cut(s) 864, 1529
Bst2UI CCWGG 5 cut(s) 669, 884, 1129, 1390, 1904
Bst4CI ACNGT 4 cut(s) 34, 776, 1354, 1405
Bst6I CTCTTC 1 cut(s) 1199
BstACI GRCGYC 3 cut(s) 381, 429, 735
BstAPI GCANNNNNTGC 1 cut(s) 1805
BstBAI YACGTR 1 cut(s) 1560
BstBI TTCGAA 1 cut(s) 1202
BstC8I GCNNGC 5 cut(s) 150, 486, 824, 849, 1833
BstDEI CTNAG 6 cut(s) 91, 456, 834, 1032, 1266, 1855
BstDSI CCRYGG 4 cut(s) 24, 30, 586, 1949
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 6 cut(s) 240, 255, 799, 1030, 1633, 1916
BstH2I RGCGCY 2 cut(s) 738, 764
BstHHI GCGC 2 cut(s) 737, 763
BstKTI GATC 9 cut(s) 355, 512, 552, 653, 726, 1060, 1861, 1867, 1939
BstMAI GTCTC 5 cut(s) 177, 222, 509, 526, 1456
BstMBI GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
BstMCI CGRYCG 4 cut(s) 145, 512, 547, 747
BstNI CCWGG 5 cut(s) 669, 884, 1129, 1390, 1904
BstNSI RCATGY 3 cut(s) 956, 1324, 1751
BstPAI GACNNNNGTC 1 cut(s) 432
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 7 cut(s) 667, 701, 756, 882, 1127, 1388, 1902
BstSFI CTRYAG 1 cut(s) 1683
BstSLI GKGCMC 2 cut(s) 621, 1106
BstV1I GCAGC 5 cut(s) 702, 1317, 1406, 1618, 1693
BstX2I RGATCY 2 cut(s) 650, 1936
BstXI CCANNNNNNTGG 2 cut(s) 685, 1950
BstYI RGATCY 2 cut(s) 650, 1936
Bsu15I ATCGAT 1 cut(s) 726
Bsu36I CCTNAGG 1 cut(s) 1266
BsuRI GGCC 8 cut(s) 29, 108, 288, 404, 619, 756, 1428, 1588
BsuTUI ATCGAT 1 cut(s) 726
BtgI CCRYGG 4 cut(s) 24, 30, 586, 1949
BtrI CACGTC 2 cut(s) 274, 364
BtsCI GGATG 6 cut(s) 240, 255, 799, 1030, 1633, 1916
BtsI GCAGTG 2 cut(s) 1386, 1980
BtsIMutI CAGTG 2 cut(s) 1386, 1980
Cac8I GCNNGC 5 cut(s) 150, 486, 824, 849, 1833
CaiI CAGNNNCTG 2 cut(s) 1232, 1805
CciI TCATGA 1 cut(s) 1861
CfoI GCGC 2 cut(s) 737, 763
Cfr10I RCCGGY 3 cut(s) 144, 580, 742
ClaI ATCGAT 1 cut(s) 726
CpoI CGGWCCG 2 cut(s) 709, 740
CseI GACGC 1 cut(s) 389
CsiI ACCWGGT 1 cut(s) 882
Csp6I GTAC 3 cut(s) 1619, 1760, 1783
CspAI ACCGGT 1 cut(s) 742
CspI CGGWCCG 2 cut(s) 709, 740
CviAII CATG 8 cut(s) 13, 953, 1321, 1433, 1712, 1748, 1862, 1895
CviQI GTAC 3 cut(s) 1619, 1760, 1783
DdeI CTNAG 6 cut(s) 91, 456, 834, 1032, 1266, 1855
DinI GGCGCC 1 cut(s) 736
DpnI GATC 9 cut(s) 354, 511, 551, 652, 725, 1059, 1860, 1866, 1938
DpnII GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
DraIII CACNNNGTG 2 cut(s) 681, 1715
EaeI YGGCCR 3 cut(s) 27, 402, 1426
Eam1104I CTCTTC 1 cut(s) 1199
EarI CTCTTC 1 cut(s) 1199
EciI GGCGGA 6 cut(s) 10, 179, 266, 298, 325, 866
Ecl136II GAGCTC 1 cut(s) 484
Eco130I CCWWGG 2 cut(s) 864, 1529
Eco24I GRGCYC 3 cut(s) 486, 621, 1529
Eco31I GGTCTC 1 cut(s) 1456
Eco47I GGWCC 7 cut(s) 583, 699, 709, 740, 1014, 1386, 1851
Eco53kI GAGCTC 1 cut(s) 484
Eco72I CACGTG 1 cut(s) 1560
Eco81I CCTNAGG 1 cut(s) 1266
Eco91I GGTNACC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 484
EcoO109I RGGNCCY 4 cut(s) 617, 618, 1014, 1851
EcoO65I GGTNACC 1 cut(s) 34
EcoRI GAATTC 2 cut(s) 909, 928
EcoRII CCWGG 5 cut(s) 667, 882, 1127, 1388, 1902
EcoT14I CCWWGG 2 cut(s) 864, 1529
EcoT38I GRGCYC 3 cut(s) 486, 621, 1529
EgeI GGCGCC 1 cut(s) 736
EheI GGCGCC 1 cut(s) 736
ErhI CCWWGG 2 cut(s) 864, 1529
Esp3I CGTCTC 2 cut(s) 222, 526
FaeI CATG 8 cut(s) 16, 956, 1324, 1436, 1715, 1751, 1865, 1898
FalI AAGNNNNNCTT 2 cut(s) 548, 580
FaqI GGGAC 1 cut(s) 118
FatI CATG 8 cut(s) 12, 952, 1320, 1432, 1711, 1747, 1861, 1894
FauI CCCGC 3 cut(s) 143, 382, 505
FauNDI CATATG 1 cut(s) 1515
FblI GTMKAC 1 cut(s) 747
Fnu4HI GCNGC 9 cut(s) 384, 515, 691, 810, 1331, 1420, 1429, 1632, 1707
FokI GGATG 6 cut(s) 227, 242, 786, 1037, 1640, 1903
FriOI GRGCYC 3 cut(s) 486, 621, 1529
Fsp4HI GCNGC 9 cut(s) 384, 515, 691, 810, 1331, 1420, 1429, 1632, 1707
FspBI CTAG 3 cut(s) 473, 1530, 2007
GlaI GCGC 2 cut(s) 736, 762
GluI GCNGC 9 cut(s) 384, 515, 691, 810, 1331, 1420, 1429, 1632, 1707
HaeII RGCGCY 2 cut(s) 738, 764
HaeIII GGCC 8 cut(s) 29, 108, 288, 404, 619, 756, 1428, 1588
HapII CCGG 8 cut(s) 145, 547, 581, 702, 738, 743, 758, 768
HgaI GACGC 1 cut(s) 389
HhaI GCGC 2 cut(s) 737, 763
Hin1I GRCGYC 3 cut(s) 381, 429, 735
Hin1II CATG 8 cut(s) 16, 956, 1324, 1436, 1715, 1751, 1865, 1898
Hin6I GCGC 2 cut(s) 735, 761
HinP1I GCGC 2 cut(s) 735, 761
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HpaII CCGG 8 cut(s) 145, 547, 581, 702, 738, 743, 758, 768
Hpy166II GTNNAC 4 cut(s) 586, 595, 748, 1440
Hpy188III TCNNGA 7 cut(s) 568, 1075, 1265, 1694, 1732, 1862, 2007
Hpy8I GTNNAC 4 cut(s) 586, 595, 748, 1440
Hpy99I CGWCG 6 cut(s) 287, 383, 431, 530, 776, 1153
HpyAV CCTTC 9 cut(s) 127, 175, 300, 304, 309, 337, 832, 1618, 1994
HpyCH4III ACNGT 4 cut(s) 34, 776, 1354, 1405
HpyCH4IV ACGT 8 cut(s) 273, 363, 429, 1148, 1175, 1544, 1559, 1738
HpyCH4V TGCA 8 cut(s) 817, 1184, 1213, 1379, 1422, 1709, 1808, 1973
HpyF3I CTNAG 6 cut(s) 91, 456, 834, 1032, 1266, 1855
HpySE526I ACGT 8 cut(s) 273, 363, 429, 1148, 1175, 1544, 1559, 1738
Hsp92I GRCGYC 3 cut(s) 381, 429, 735
Hsp92II CATG 8 cut(s) 16, 956, 1324, 1436, 1715, 1751, 1865, 1898
HspAI GCGC 2 cut(s) 735, 761
KasI GGCGCC 1 cut(s) 734
Kzo9I GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
LmnI GCTCC 3 cut(s) 1453, 1524, 1734
Lsp1109I GCAGC 5 cut(s) 702, 1317, 1406, 1618, 1693
LweI GCATC 3 cut(s) 1222, 1618, 1925
MabI ACCWGGT 1 cut(s) 882
MaeI CTAG 3 cut(s) 473, 1530, 2007
MaeII ACGT 8 cut(s) 273, 363, 429, 1148, 1175, 1544, 1559, 1738
MaeIII GTNAC 4 cut(s) 34, 434, 656, 1812
MalI GATC 9 cut(s) 354, 511, 551, 652, 725, 1059, 1860, 1866, 1938
MbiI CCGCTC 1 cut(s) 517
MboI GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
MboII GAAGA 3 cut(s) 881, 1216, 1768
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 2 cut(s) 650, 1936
MhlI GDGCHC 4 cut(s) 486, 621, 1106, 1529
Mly113I GGCGCC 1 cut(s) 735
MlyI GAGTC 7 cut(s) 155, 383, 530, 653, 1223, 1504, 1570
MmeI TCCRAC 2 cut(s) 180, 687
MroXI GAANNNNTTC 1 cut(s) 1775
MseI TTAA 1 cut(s) 1137
MslI CAYNNNNRTG 3 cut(s) 11, 957, 1948
MspI CCGG 8 cut(s) 145, 547, 581, 702, 738, 743, 758, 768
MspR9I CCNGG 7 cut(s) 669, 703, 758, 884, 1129, 1390, 1904
MunI CAATTG 1 cut(s) 1005
MvaI CCWGG 5 cut(s) 669, 884, 1129, 1390, 1904
NarI GGCGCC 1 cut(s) 735
NciI CCSGG 2 cut(s) 703, 758
NdeI CATATG 1 cut(s) 1515
NdeII GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
NlaIII CATG 8 cut(s) 16, 956, 1324, 1436, 1715, 1751, 1865, 1898
NlaIV GGNNCC 6 cut(s) 618, 619, 620, 736, 1526, 1938
NmuCI GTSAC 4 cut(s) 34, 434, 656, 1812
NspI RCATGY 3 cut(s) 956, 1324, 1751
NspV TTCGAA 1 cut(s) 1202
PagI TCATGA 1 cut(s) 1861
PciI ACATGT 2 cut(s) 952, 1747
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 540, 1103
PdmI GAANNNNTTC 1 cut(s) 1775
PfeI GAWTC 3 cut(s) 980, 1199, 1697
PflFI GACNNNGTC 2 cut(s) 703, 776
PflMI CCANNNNNTGG 2 cut(s) 1472, 1909
PinAI ACCGGT 1 cut(s) 742
PkrI GCNGC 9 cut(s) 385, 516, 692, 811, 1332, 1421, 1430, 1633, 1708
Ple19I CGATCG 1 cut(s) 512
PleI GAGTC 7 cut(s) 155, 383, 530, 653, 1223, 1504, 1570
PluTI GGCGCC 1 cut(s) 738
PmaCI CACGTG 1 cut(s) 1560
PmlI CACGTG 1 cut(s) 1560
PpsI GAGTC 7 cut(s) 155, 383, 530, 653, 1223, 1504, 1570
Ppu21I YACGTR 1 cut(s) 1560
PpuMI RGGWCCY 2 cut(s) 1014, 1851
PscI ACATGT 2 cut(s) 952, 1747
PshAI GACNNNNGTC 1 cut(s) 432
PshBI ATTAAT 1 cut(s) 1137
PsiI TTATAA 1 cut(s) 1083
Psp124BI GAGCTC 1 cut(s) 486
Psp5II RGGWCCY 2 cut(s) 1014, 1851
Psp6I CCWGG 5 cut(s) 667, 882, 1127, 1388, 1902
PspCI CACGTG 1 cut(s) 1560
PspEI GGTNACC 1 cut(s) 34
PspGI CCWGG 5 cut(s) 667, 882, 1127, 1388, 1902
PspN4I GGNNCC 6 cut(s) 618, 619, 620, 736, 1526, 1938
PspOMI GGGCCC 1 cut(s) 617
PspPPI RGGWCCY 2 cut(s) 1014, 1851
PstNI CAGNNNCTG 2 cut(s) 1232, 1805
PsuI RGATCY 2 cut(s) 650, 1936
PsyI GACNNNGTC 2 cut(s) 703, 776
PvuI CGATCG 1 cut(s) 512
RsaI GTAC 3 cut(s) 1620, 1761, 1784
RsaNI GTAC 3 cut(s) 1619, 1760, 1783
RseI CAYNNNNRTG 3 cut(s) 11, 957, 1948
Rsr2I CGGWCCG 2 cut(s) 709, 740
RsrII CGGWCCG 2 cut(s) 709, 740
SacI GAGCTC 1 cut(s) 486
SalI GTCGAC 1 cut(s) 746
SaqAI TTAA 1 cut(s) 1137
SatI GCNGC 9 cut(s) 384, 515, 691, 810, 1331, 1420, 1429, 1632, 1707
Sau3AI GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
SchI GAGTC 7 cut(s) 155, 383, 530, 653, 1223, 1504, 1570
ScrFI CCNGG 7 cut(s) 669, 703, 758, 884, 1129, 1390, 1904
SduI GDGCHC 4 cut(s) 486, 621, 1106, 1529
SexAI ACCWGGT 1 cut(s) 882
SfaNI GCATC 3 cut(s) 1222, 1618, 1925
SfcI CTRYAG 1 cut(s) 1683
SfoI GGCGCC 1 cut(s) 736
SfuI TTCGAA 1 cut(s) 1202
SinI GGWCC 7 cut(s) 583, 699, 709, 740, 1014, 1386, 1851
SmiMI CAYNNNNRTG 3 cut(s) 11, 957, 1948
SmlI CTYRAG 1 cut(s) 1457
SmoI CTYRAG 1 cut(s) 1457
SspDI GGCGCC 1 cut(s) 734
SspMI CTAG 3 cut(s) 473, 1530, 2007
SstI GAGCTC 1 cut(s) 486
StyD4I CCNGG 7 cut(s) 667, 701, 756, 882, 1127, 1388, 1902
StyI CCWWGG 2 cut(s) 864, 1529
TaaI ACNGT 4 cut(s) 34, 776, 1354, 1405
TaiI ACGT 8 cut(s) 276, 366, 432, 1151, 1178, 1547, 1562, 1741
TatI WGTACW 1 cut(s) 1782
TauI GCSGC 4 cut(s) 386, 517, 812, 1431
TfiI GAWTC 3 cut(s) 980, 1199, 1697
Tru1I TTAA 1 cut(s) 1137
Tru9I TTAA 1 cut(s) 1137
TscAI CASTG 2 cut(s) 1386, 1980
TseFI GTSAC 4 cut(s) 34, 434, 656, 1812
TseI GCWGC 5 cut(s) 690, 1330, 1419, 1631, 1706
Tsp45I GTSAC 4 cut(s) 34, 434, 656, 1812
TspDTI ATGAA 4 cut(s) 788, 1104, 1131, 1593
TspGWI ACGGA 2 cut(s) 514, 1938
TspRI CASTG 2 cut(s) 1386, 1980
Tth111I GACNNNGTC 2 cut(s) 703, 776
Van91I CCANNNNNTGG 2 cut(s) 1472, 1909
VpaK11BI GGWCC 7 cut(s) 583, 699, 709, 740, 1014, 1386, 1851
VspI ATTAAT 1 cut(s) 1137
XapI RAATTY 3 cut(s) 909, 928, 1771
XbaI TCTAGA 1 cut(s) 2006
XceI RCATGY 3 cut(s) 956, 1324, 1751
XcmI CCANNNNNNNNNTGG 1 cut(s) 1947
XmaJI CCTAGG 1 cut(s) 1529
XmiI GTMKAC 1 cut(s) 747
XmnI GAANNNNTTC 1 cut(s) 1775
XspI CTAG 3 cut(s) 473, 1530, 2007
ZraI GACGTC 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.