pycom111g03860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0012436
Physical Location & Seq
Reverse (-)
2337336 .. 2338364
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03860.1

Sequence Viewer

Length: 1029 bp
ATGGATGAGGCAAATAGTAATGGTTTGTTGTTAGGGGGTCAGAGTTTGAGTTTCAGGACTAATAGGGTGAGTTACTCAGATTGCCCGATTTGTTCATTTGCGATTTCGAGGTCTATTAACTCGTATACATCGAGGTTCTTGTTTGATAACTATACTTTGATTGCAAGTGAGTATTTGGACTCGAAGCGATTGCATCAAATATTGTCTGATTCGGCTGACGAGTTTAGGAGAGTGGCAGGGTTCGCTGAGGAGGATTTTGGTCGGGTTCTTCCTGTTTATGTGTTTGATTTGGACTACAACATTCTGTTGTTGCTCGATAGGTATCACCAATCGGTTGCTTTCAAAGACATGGTTATTGCAGTGAGGACAAAGAGCACGCAGACTGTGAGTGATTATAGCTGTAATGGGCATCATGTGTTTACGCAAACGAGAGAGCTTGAGAGACCACTTGTTGGTTCAATTTTGCAGAGCATGTGGGGAGTGTCACCAACCCATATGCTCTGGAGCCCTAGGCACAATACTACATTGGTGGATTACACATGGAGTATCGGCCAGACTCCGTTTGGGCCATTCTCTGAGATTTCATCCTTATCATTTGTGCAGAAGGATGCTGCTCGGAGAAATGTTCTTTTGACATCAATGAATTACAGCATATCAACTGCTGTAGATGTTCTCGAATCCTTAGCTGCACATGGTGGGGAAAGAAAGCTCCTTAAGCGTAGTCAACATGTTGAGTTCATACAGAGGTGGAACTTGTTCAAGTATAAGCTGGACAAATCAGTCTCTGCAATGTCACTTTTGGATTTCGAGATGGCTTTGTTTTATTTGCGGGTCTCAGACCACGACCTATACGCCATGCAGTCTCTCGTCTATCAAGCCACCCAAGACGTGGAGGCATCCCTTGTATGTTTCAAGGACCCGCCCATTCCATGGAACGGGATCTTTATTGTGGCATTTGTTATCGTTTTTGCCTTTTATCTTCATTCCAAACGAGATAAAGTCTTTCGAAATAAACGTAAGCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

343

Amino Acids

39.3

Weight (kDa)

7.72

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 779
AccB7I CCANNNNNTGG 3 cut(s) 452, 889, 930
AccI GTMKAC 1 cut(s) 125
AciI CCGC 2 cut(s) 829, 920
AclWI GGATC 1 cut(s) 947
AcoI YGGCCR 1 cut(s) 550
AdeI CACNNNGTG 1 cut(s) 695
AfiI CCNNNNNNNGG 4 cut(s) 452, 889, 930, 935
AflII CTTAAG 1 cut(s) 713
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 4 cut(s) 343, 459, 760, 913
AjiI CACGTC 1 cut(s) 889
AluBI AGCT 5 cut(s) 399, 436, 686, 709, 769
AluI AGCT 5 cut(s) 399, 436, 686, 709, 769
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 4 cut(s) 436, 787, 838, 867
AlwI GGATC 1 cut(s) 947
AlwNI CAGNNNCTG 1 cut(s) 785
AoxI GGCC 2 cut(s) 550, 566
ApeKI GCWGC 2 cut(s) 611, 686
Asp700I GAANNNNTTC 1 cut(s) 755
AspA2I CCTAGG 1 cut(s) 509
AspS9I GGNCC 2 cut(s) 566, 916
AsuHPI GGTGA 3 cut(s) 79, 317, 477
AsuII TTCGAA 1 cut(s) 1006
AvaII GGWCC 1 cut(s) 916
AvrII CCTAGG 1 cut(s) 509
BanII GRGCYC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 377
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 2 cut(s) 598, 673
BccI CCATC 1 cut(s) 805
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BcoDI GTCTC 4 cut(s) 436, 787, 838, 867
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 1 cut(s) 663
BfrI CTTAAG 1 cut(s) 713
BisI GCNGC 2 cut(s) 612, 687
BlnI CCTAGG 1 cut(s) 509
BlsI GCNGC 2 cut(s) 613, 688
Bme18I GGWCC 1 cut(s) 916
BmgBI CACGTC 1 cut(s) 889
BmgT120I GGNCC 2 cut(s) 566, 916
BmiI GGNNCC 2 cut(s) 506, 918
BmsI GCATC 4 cut(s) 202, 418, 598, 905
BpmI CTGGAG 1 cut(s) 523
Bpu10I CCTNAGC 2 cut(s) 246, 682
Bpu14I TTCGAA 1 cut(s) 1006
BpuEI CTTGAG 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 321
BsaI GGTCTC 2 cut(s) 436, 838
BsaJI CCNNGG 2 cut(s) 509, 929
Bsc4I CCNNNNNNNGG 4 cut(s) 452, 889, 930, 935
Bse3DI GCAATG 1 cut(s) 795
Bse8I GATNNNNATC 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 509, 929
BseGI GGATG 4 cut(s) 10, 584, 613, 896
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 4 cut(s) 452, 889, 930, 935
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 4 cut(s) 90, 237, 567, 849
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 2 cut(s) 598, 673
BsgI GTGCAG 2 cut(s) 620, 672
BshFI GGCC 2 cut(s) 552, 568
BsiHKAI GWGCWC 1 cut(s) 377
BslI CCNNNNNNNGG 4 cut(s) 452, 889, 930, 935
BsmAI GTCTC 4 cut(s) 436, 787, 838, 867
BsnI GGCC 2 cut(s) 552, 568
Bso31I GGTCTC 2 cut(s) 436, 838
Bsp119I TTCGAA 1 cut(s) 1006
Bsp1286I GDGCHC 2 cut(s) 377, 509
Bsp143I GATC 1 cut(s) 939
Bsp19I CCATGG 1 cut(s) 929
BspACI CCGC 2 cut(s) 829, 920
BspANI GGCC 2 cut(s) 552, 568
BspCNI CTCAG 4 cut(s) 89, 238, 568, 848
BspLI GGNNCC 2 cut(s) 506, 918
BspPI GGATC 1 cut(s) 947
BspT104I TTCGAA 1 cut(s) 1006
BspTI CTTAAG 1 cut(s) 713
BspTNI GGTCTC 2 cut(s) 436, 838
BsrDI GCAATG 1 cut(s) 795
BssECI CCNNGG 2 cut(s) 509, 929
BssMI GATC 1 cut(s) 939
BssNAI GTATAC 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 509, 929
Bst1107I GTATAC 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 385
BstAFI CTTAAG 1 cut(s) 713
BstBI TTCGAA 1 cut(s) 1006
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 5 cut(s) 76, 246, 576, 682, 835
BstDSI CCRYGG 1 cut(s) 929
BstF5I GGATG 4 cut(s) 10, 584, 613, 896
BstKTI GATC 1 cut(s) 942
BstMAI GTCTC 4 cut(s) 436, 787, 838, 867
BstMBI GATC 1 cut(s) 939
BstMWI GCNNNNNNNGC 2 cut(s) 242, 715
BstNSI RCATGY 2 cut(s) 475, 731
BstSFI CTRYAG 1 cut(s) 663
BstV1I GCAGC 2 cut(s) 598, 673
BstX2I RGATCY 1 cut(s) 939
BstYI RGATCY 1 cut(s) 939
BstZ17I GTATAC 1 cut(s) 126
BsuRI GGCC 2 cut(s) 552, 568
BtgI CCRYGG 1 cut(s) 929
BtrI CACGTC 1 cut(s) 889
BtsCI GGATG 4 cut(s) 10, 584, 613, 896
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 1 cut(s) 377
CaiI CAGNNNCTG 1 cut(s) 785
Cfr13I GGNCC 2 cut(s) 566, 916
CviAII CATG 8 cut(s) 349, 413, 472, 540, 692, 728, 856, 930
DdeI CTNAG 5 cut(s) 76, 246, 576, 682, 835
DpnI GATC 1 cut(s) 941
DpnII GATC 1 cut(s) 939
DraIII CACNNNGTG 1 cut(s) 695
DrdI GACNNNNNNGTC 1 cut(s) 779
DseDI GACNNNNNNGTC 1 cut(s) 779
EaeI YGGCCR 1 cut(s) 550
Eco130I CCWWGG 2 cut(s) 509, 929
Eco24I GRGCYC 1 cut(s) 509
Eco31I GGTCTC 2 cut(s) 436, 838
Eco47I GGWCC 1 cut(s) 916
EcoO109I RGGNCCY 1 cut(s) 916
EcoT14I CCWWGG 2 cut(s) 509, 929
EcoT38I GRGCYC 1 cut(s) 509
ErhI CCWWGG 2 cut(s) 509, 929
FaeI CATG 8 cut(s) 352, 416, 475, 543, 695, 731, 859, 933
FatI CATG 8 cut(s) 348, 412, 471, 539, 691, 727, 855, 929
FauI CCCGC 2 cut(s) 822, 927
FauNDI CATATG 1 cut(s) 495
FblI GTMKAC 1 cut(s) 125
Fnu4HI GCNGC 2 cut(s) 612, 687
FokI GGATG 4 cut(s) 17, 571, 620, 883
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 2 cut(s) 612, 687
FspBI CTAG 1 cut(s) 510
GluI GCNGC 2 cut(s) 612, 687
GsuI CTGGAG 1 cut(s) 523
HaeIII GGCC 2 cut(s) 552, 568
Hin1II CATG 8 cut(s) 352, 416, 475, 543, 695, 731, 859, 933
HincII GTYRAC 1 cut(s) 725
HindII GTYRAC 1 cut(s) 725
HinfI GANTC 4 cut(s) 179, 209, 556, 677
HphI GGTGA 3 cut(s) 79, 317, 477
Hpy166II GTNNAC 3 cut(s) 126, 420, 725
Hpy188I TCNGA 6 cut(s) 42, 79, 208, 577, 618, 838
Hpy188III TCNNGA 4 cut(s) 55, 502, 674, 808
Hpy8I GTNNAC 3 cut(s) 126, 420, 725
HpyAV CCTTC 1 cut(s) 598
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 888, 1015
HpyCH4V TGCA 8 cut(s) 164, 193, 359, 466, 601, 689, 788, 859
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 715
HpyF3I CTNAG 5 cut(s) 76, 246, 576, 682, 835
HpySE526I ACGT 2 cut(s) 888, 1015
Hsp92II CATG 8 cut(s) 352, 416, 475, 543, 695, 731, 859, 933
Kzo9I GATC 1 cut(s) 939
LmnI GCTCC 2 cut(s) 504, 714
LpnPI CCDG 6 cut(s) 40, 222, 285, 487, 566, 755
Lsp1109I GCAGC 2 cut(s) 598, 673
LweI GCATC 4 cut(s) 202, 418, 598, 905
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 888, 1015
MaeIII GTNAC 3 cut(s) 71, 483, 792
MalI GATC 1 cut(s) 941
MboI GATC 1 cut(s) 939
MboII GAAGA 2 cut(s) 260, 971
MflI RGATCY 1 cut(s) 939
MhlI GDGCHC 2 cut(s) 377, 509
MluCI AATT 3 cut(s) 459, 643, 1022
MlyI GAGTC 2 cut(s) 173, 550
MnlI CCTC 7 cut(s) 102, 126, 241, 244, 357, 738, 886
MroXI GAANNNNTTC 1 cut(s) 755
MseI TTAA 2 cut(s) 117, 714
MspCI CTTAAG 1 cut(s) 713
MwoI GCNNNNNNNGC 2 cut(s) 242, 715
NcoI CCATGG 1 cut(s) 929
NdeI CATATG 1 cut(s) 495
NdeII GATC 1 cut(s) 939
NlaIII CATG 8 cut(s) 352, 416, 475, 543, 695, 731, 859, 933
NlaIV GGNNCC 2 cut(s) 506, 918
NmuCI GTSAC 2 cut(s) 483, 792
NspI RCATGY 2 cut(s) 475, 731
NspV TTCGAA 1 cut(s) 1006
PciI ACATGT 1 cut(s) 727
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 1012
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 2 cut(s) 209, 677
PflMI CCANNNNNTGG 3 cut(s) 452, 889, 930
PkrI GCNGC 2 cut(s) 613, 688
PleI GAGTC 2 cut(s) 173, 550
PpsI GAGTC 2 cut(s) 173, 550
PpuMI RGGWCCY 1 cut(s) 916
PscI ACATGT 1 cut(s) 727
Psp5II RGGWCCY 1 cut(s) 916
PspN4I GGNNCC 2 cut(s) 506, 918
PspPI GGNCC 2 cut(s) 566, 916
PspPPI RGGWCCY 1 cut(s) 916
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 1 cut(s) 939
SaqAI TTAA 2 cut(s) 117, 714
SatI GCNGC 2 cut(s) 612, 687
Sau3AI GATC 1 cut(s) 939
Sau96I GGNCC 2 cut(s) 566, 916
SchI GAGTC 2 cut(s) 173, 550
SduI GDGCHC 2 cut(s) 377, 509
SfaNI GCATC 4 cut(s) 202, 418, 598, 905
SfcI CTRYAG 1 cut(s) 663
SfuI TTCGAA 1 cut(s) 1006
SinI GGWCC 1 cut(s) 916
SmlI CTYRAG 2 cut(s) 437, 713
SmoI CTYRAG 2 cut(s) 437, 713
Sse9I AATT 3 cut(s) 459, 643, 1022
SsiI CCGC 2 cut(s) 829, 920
SspI AATATT 1 cut(s) 201
SspMI CTAG 1 cut(s) 510
StyI CCWWGG 2 cut(s) 509, 929
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 891, 1018
TaqI TCGA 7 cut(s) 107, 131, 182, 315, 675, 807, 1006
TasI AATT 3 cut(s) 459, 643, 1022
TfiI GAWTC 2 cut(s) 209, 677
Tru1I TTAA 2 cut(s) 117, 714
Tru9I TTAA 2 cut(s) 117, 714
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 2 cut(s) 483, 792
TseI GCWGC 2 cut(s) 611, 686
Tsp45I GTSAC 2 cut(s) 483, 792
TspDTI ATGAA 5 cut(s) 84, 573, 656, 727, 971
TspGWI ACGGA 1 cut(s) 549
TspRI CASTG 1 cut(s) 366
Van91I CCANNNNNTGG 3 cut(s) 452, 889, 930
Vha464I CTTAAG 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 916
XceI RCATGY 2 cut(s) 475, 731
XcmI CCANNNNNNNNNTGG 2 cut(s) 560, 886
XmaJI CCTAGG 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 755
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.