MD09G1047700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012436
Physical Location & Seq
Reverse (-)
3131218 .. 3133318
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1047700.v1.1.491

Sequence Viewer

Length: 2052 bp
ATGACCGCCATGGCCACCACCATGGCCGCCACCACCACTCTCCTCCTTCTCCTGATCCTAACCGCCGCCATAACCACCGCCAGCTCAGCTCCGATCCTCGGTCTCGACACCATCCTCACCCACCAATCCCGCCTTGACCGCCATGCCACCAACGACTCCTTCCTCTCTCTCTCCTCCTCCCTCAAAAACTCCCTTTCCCATTCTTCCCCTCACTCCCACGCCCCCCACTCCCTCATCTCTTCCCTCCTCTCCCTCTCCCTCTCTCTCTCACTCCACGTCCGCCTCGTCGGCCCCTCCTTCCCCTCCAGCTCCAGCGCCCTCCTCTCCTCCTTCCTCTCCGCCGCCTCCCAACCCTCCAACCACTTCCACGTCATCGCGCCCTACGAATCCCTCTCCCACCGCATCGCCGTCCAGCACTCTCTCCACCTGGGCGTCTCTCATTCCCCCTCTCTCGCCTCCCTCCTCACTAAAACCCTAAGCTCCGAGCTCGCCAAAACCCCTTCCAGCCTCCGATCGCCGCTCATCTCAATCCCCTACGACTCAATCGACCAAATCATCAAGCAGGACTTCGAGAAGGAGAAGCCGGTTCATGGGGTTTACTTATATTTTCTCGATTTGGGTCAGCAATCCAAACCCTATGCTTACAATTACGGATCTGGGGACTCTTCCCCTGCATTCACCAAGTGTTTGGGCAGCATTTGGACCGGGAAAGACCGGTATATCTGGATCGATTTGGGCGCCGGACCTGTCGATTATGGCCCGGGGCTTTCAGGTGATGGGGTTTTGCCCAGAGGCGAATTTCATCCGTTGGCGGCGTTGCACGGCCGCCCAAGGTCTCAAAAGGCGTTGCTTGCTAACTTGGCATCCTTGGTTTGGAGTGCTTACCAGGTTCTTCTTGTTCCCTCTTTGAGAATTCCTGTTCAGTTTGAGAATTCATTGATTGTTCAGTTTATACATGTTTATGGGTCCGATGGTGGTAAGGACTCGAGTGGTTTGGATTGGAAATCGATTGAGAGGACCTTTATGGATGAGGCAAATAGTAATGGTTTGTTGTTAGGGGGTCAGAGTTTGAGTTTCAGGACTAATAGGGTGAGTTACTCAGATTGCCCGATTTGTTCATTTGCGATTTCGAGGTCTATTAACTCGTATACATCGAGGTTCTTGTTTGATAACTATACTTTGATTGCAAGTGAGTATTTGGACTCGAAGCGGTTGCATCAAATATTGTCTGATTCGGCTGACGAGTTTAGGAGAGTGGCAGGGTTCGCTGAGGAGGATTTTGGTCGGGTTCTTCCTGTTTATGTGTTTGATTTGGACTACAACATGCTGTTGTTGCTCGATAGGTATCACCAATCGGTTGCTTTCAAAGACATGGTTATTGCAGTGAGGACAAAGAGCACGCAGACTGTGAGTGATTATAGCTGTAACGGGCATCATGTGTTTACGCAAACAAGAGAGCTTGAGAGACCGCTTGTTGGTTCAATTCTGCAGAGCATGTGGGGAGTGTCACCAACCCATATGCTCTGGAGCCCTAGGCACAATACTACATTGGTGGATTACACATGGAGTATCGGCCAGACTCCATTTGGGCCATTCTCTGAGATTTCATCCTTATCATTTGTGCAGAAGGATGCTGCTCGGAGAAATGTTCTTTTGACAACAATGAATTACAGCATATCAACTGCTGTAGATGTTCTCGAATCCTTAGTTGCACATGGTGGGGAAAGAAAGCTCCTCAAGCGTAGTCAACACGTTGAGTTCATACAGAGGTGGAACTTGTTCAAGTATAAGCTGGACAAATCAGTCTCTGCAATGTCACTTTTGGATTTCGAGATGGCTTTGTTTTATTTGCAGGCCTCAGACCACGACCTATACGCCATGCAGTCTCTCGTCTATCAAGCCACCCAAGACGTGGAGGCGTCCCTTGTATGTTTCAAGGACCCACCCGTTCCATGGAACGGGATCTTTATGGTGGCATTTGTTATCGTTTTTGCCTTTTATCTTCATTCTAAACGAGATAAAGTCTTTCGAAATAAAAGTAAGCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

684

Amino Acids

75.89

Weight (kDa)

7.42

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 88 - 301 1.4e-85 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1802
AccB1I GGYRCC 1 cut(s) 737
AccB7I CCANNNNNTGG 1 cut(s) 1912
AccBSI CCGCTC 1 cut(s) 520
AccI GTMKAC 1 cut(s) 1148
AccII CGCG 1 cut(s) 377
AclWI GGATC 5 cut(s) 49, 88, 661, 734, 1970
AcoI YGGCCR 4 cut(s) 12, 24, 823, 1573
AcsI RAATTY 3 cut(s) 797, 912, 931
AcyI GRCGYC 3 cut(s) 432, 738, 1919
AdeI CACNNNGTG 2 cut(s) 684, 1718
AfiI CCNNNNNNNGG 7 cut(s) 98, 590, 873, 1475, 1912, 1953, 1958
AflIII ACRYGT 2 cut(s) 955, 1750
AgeI ACCGGT 1 cut(s) 714
AgsI TTSAA 4 cut(s) 1366, 1482, 1783, 1936
AjiI CACGTC 3 cut(s) 277, 370, 1912
AjnI CCWGG 2 cut(s) 426, 885
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 9 cut(s) 84, 89, 309, 480, 487, 1422, 1459, 1732, 1792
AluI AGCT 9 cut(s) 84, 89, 309, 480, 487, 1422, 1459, 1732, 1792
Alw21I GWGCWC 2 cut(s) 489, 1400
Alw26I GTCTC 6 cut(s) 107, 439, 840, 1459, 1810, 1890
AlwI GGATC 5 cut(s) 49, 88, 661, 734, 1970
AlwNI CAGNNNCTG 1 cut(s) 1808
Ama87I CYCGRG 2 cut(s) 760, 985
AoxI GGCC 8 cut(s) 12, 24, 289, 757, 823, 1573, 1589, 1854
ApeKI GCWGC 2 cut(s) 693, 1634
ApoI RAATTY 3 cut(s) 797, 912, 931
AsiGI ACCGGT 1 cut(s) 714
Asp700I GAANNNNTTC 1 cut(s) 1778
AspA2I CCTAGG 1 cut(s) 1532
AspLEI GCGC 3 cut(s) 317, 379, 740
AspS9I GGNCC 8 cut(s) 290, 702, 743, 758, 966, 1017, 1589, 1939
AsuC2I CCSGG 3 cut(s) 706, 761, 762
AsuHPI GGTGA 6 cut(s) 109, 670, 785, 1102, 1340, 1500
AsuII TTCGAA 1 cut(s) 2029
AvaI CYCGRG 2 cut(s) 760, 985
AvaII GGWCC 5 cut(s) 702, 743, 966, 1017, 1939
AvrII CCTAGG 1 cut(s) 1532
BalI TGGCCA 1 cut(s) 14
BanI GGYRCC 1 cut(s) 737
BanII GRGCYC 2 cut(s) 489, 1532
Bbv12I GWGCWC 2 cut(s) 489, 1400
BbvCI CCTCAGC 1 cut(s) 1269
BbvI GCAGC 2 cut(s) 705, 1621
BccI CCATC 4 cut(s) 119, 770, 965, 1828
BceAI ACGGC 2 cut(s) 392, 838
BcgI CGANNNNNNTGC 2 cut(s) 1195, 1229
BciT130I CCWGG 2 cut(s) 428, 887
BcnI CCSGG 3 cut(s) 706, 761, 762
BcoDI GTCTC 6 cut(s) 107, 439, 840, 1459, 1810, 1890
BfaI CTAG 1 cut(s) 1533
BfmI CTRYAG 2 cut(s) 1487, 1686
BfoI RGCGCY 2 cut(s) 318, 741
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 8 cut(s) 27, 66, 342, 518, 694, 813, 826, 1635
BlnI CCTAGG 1 cut(s) 1532
BlpI GCTNAGC 1 cut(s) 85
BlsI GCNGC 8 cut(s) 28, 67, 343, 519, 695, 814, 827, 1636
Bme1390I CCNGG 5 cut(s) 428, 706, 761, 762, 887
Bme18I GGWCC 5 cut(s) 702, 743, 966, 1017, 1939
BmeT110I CYCGRG 2 cut(s) 760, 985
BmgBI CACGTC 3 cut(s) 277, 370, 1912
BmgT120I GGNCC 8 cut(s) 290, 702, 743, 758, 966, 1017, 1589, 1939
BmiI GGNNCC 5 cut(s) 292, 739, 967, 1529, 1941
BmrFI CCNGG 5 cut(s) 428, 706, 761, 762, 887
BmsI GCATC 5 cut(s) 411, 872, 1225, 1441, 1621
BpmI CTGGAG 3 cut(s) 289, 295, 1546
Bpu10I CCTNAGC 2 cut(s) 476, 1269
Bpu1102I GCTNAGC 1 cut(s) 85
Bpu14I TTCGAA 1 cut(s) 2029
BpuEI CTTGAG 2 cut(s) 1481, 1721
BpuMI CCSGG 3 cut(s) 706, 761, 762
Bsa29I ATCGAT 2 cut(s) 729, 1007
BsaBI GATNNNNATC 1 cut(s) 1344
BsaHI GRCGYC 3 cut(s) 432, 738, 1919
BsaI GGTCTC 3 cut(s) 107, 840, 1459
BsaWI WCCGGW 1 cut(s) 714
Bsc4I CCNNNNNNNGG 7 cut(s) 98, 590, 873, 1475, 1912, 1953, 1958
Bse118I RCCGGY 2 cut(s) 583, 714
Bse3DI GCAATG 1 cut(s) 1818
Bse8I GATNNNNATC 1 cut(s) 1344
BseBI CCWGG 2 cut(s) 428, 887
BseCI ATCGAT 2 cut(s) 729, 1007
BseGI GGATG 6 cut(s) 111, 802, 863, 1033, 1607, 1636
BseJI GATNNNNATC 1 cut(s) 1344
BseLI CCNNNNNNNGG 7 cut(s) 98, 590, 873, 1475, 1912, 1953, 1958
BseMI GCAATG 1 cut(s) 1818
BseMII CTCAG 5 cut(s) 99, 1113, 1260, 1590, 1872
BseRI GAGGAG 9 cut(s) 32, 163, 166, 236, 311, 316, 452, 1286, 1724
BseX3I CGGCCG 1 cut(s) 823
BseXI GCAGC 2 cut(s) 705, 1621
BsgI GTGCAG 1 cut(s) 1643
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 2 cut(s) 515, 826
BshFI GGCC 8 cut(s) 14, 26, 291, 759, 825, 1575, 1591, 1856
BshNI GGYRCC 1 cut(s) 737
BshTI ACCGGT 1 cut(s) 714
BshVI ATCGAT 2 cut(s) 729, 1007
BsiEI CGRYCG 2 cut(s) 515, 826
BsiHKAI GWGCWC 2 cut(s) 489, 1400
BsiHKCI CYCGRG 2 cut(s) 760, 985
BsiSI CCGG 5 cut(s) 584, 705, 715, 741, 761
BslFI GGGAC 2 cut(s) 674, 1906
BslI CCNNNNNNNGG 7 cut(s) 98, 590, 873, 1475, 1912, 1953, 1958
BsmAI GTCTC 6 cut(s) 107, 439, 840, 1459, 1810, 1890
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 2 cut(s) 674, 1906
BsmI GAATGC 1 cut(s) 674
BsnI GGCC 8 cut(s) 14, 26, 291, 759, 825, 1575, 1591, 1856
Bso31I GGTCTC 3 cut(s) 107, 840, 1459
BsoBI CYCGRG 2 cut(s) 760, 985
Bsp119I TTCGAA 1 cut(s) 2029
Bsp1286I GDGCHC 3 cut(s) 489, 1400, 1532
Bsp143I GATC 6 cut(s) 54, 93, 512, 653, 726, 1962
Bsp1720I GCTNAGC 1 cut(s) 85
Bsp19I CCATGG 3 cut(s) 9, 21, 1952
BspANI GGCC 8 cut(s) 14, 26, 291, 759, 825, 1575, 1591, 1856
BspCNI CTCAG 5 cut(s) 98, 1112, 1261, 1591, 1871
BspDI ATCGAT 2 cut(s) 729, 1007
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 5 cut(s) 292, 739, 967, 1529, 1941
BspMAI CTGCAG 1 cut(s) 1491
BspPI GGATC 5 cut(s) 49, 88, 661, 734, 1970
BspT104I TTCGAA 1 cut(s) 2029
BspT107I GGYRCC 1 cut(s) 737
BspTNI GGTCTC 3 cut(s) 107, 840, 1459
BsrBI CCGCTC 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 1818
BsrFI RCCGGY 2 cut(s) 583, 714
BssAI RCCGGY 2 cut(s) 583, 714
BssMI GATC 6 cut(s) 54, 93, 512, 653, 726, 1962
BssNAI GTATAC 1 cut(s) 1149
BssNI GRCGYC 3 cut(s) 432, 738, 1919
BssT1I CCWWGG 6 cut(s) 9, 21, 830, 867, 1532, 1952
Bst1107I GTATAC 1 cut(s) 1149
Bst2UI CCWGG 2 cut(s) 428, 887
Bst4CI ACNGT 1 cut(s) 1408
Bst6I CTCTTC 2 cut(s) 244, 670
BstACI GRCGYC 3 cut(s) 432, 738, 1919
BstBI TTCGAA 1 cut(s) 2029
BstC8I GCNNGC 5 cut(s) 82, 489, 852, 1400, 1854
BstDEI CTNAG 7 cut(s) 85, 476, 1099, 1269, 1599, 1705, 1858
BstDSI CCRYGG 3 cut(s) 9, 21, 1952
BstF5I GGATG 6 cut(s) 111, 802, 863, 1033, 1607, 1636
BstFNI CGCG 1 cut(s) 377
BstH2I RGCGCY 2 cut(s) 318, 741
BstHHI GCGC 3 cut(s) 317, 379, 740
BstKTI GATC 6 cut(s) 57, 96, 515, 656, 729, 1965
BstMAI GTCTC 6 cut(s) 107, 439, 840, 1459, 1810, 1890
BstMBI GATC 6 cut(s) 54, 93, 512, 653, 726, 1962
BstMCI CGRYCG 2 cut(s) 515, 826
BstMWI GCNNNNNNNGC 8 cut(s) 86, 138, 288, 851, 860, 1265, 1333, 1738
BstNI CCWGG 2 cut(s) 428, 887
BstNSI RCATGY 3 cut(s) 959, 1327, 1498
BstSCI CCNGG 5 cut(s) 426, 704, 759, 760, 885
BstSFI CTRYAG 2 cut(s) 1487, 1686
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 705, 1621
BstX2I RGATCY 2 cut(s) 653, 1962
BstXI CCANNNNNNTGG 2 cut(s) 22, 688
BstYI RGATCY 2 cut(s) 653, 1962
BstZ17I GTATAC 1 cut(s) 1149
BstZI CGGCCG 1 cut(s) 823
Bsu15I ATCGAT 2 cut(s) 729, 1007
BsuRI GGCC 8 cut(s) 14, 26, 291, 759, 825, 1575, 1591, 1856
BsuTUI ATCGAT 2 cut(s) 729, 1007
BtgI CCRYGG 3 cut(s) 9, 21, 1952
BtgZI GCGATG 2 cut(s) 358, 388
BtrI CACGTC 3 cut(s) 277, 370, 1912
BtsCI GGATG 6 cut(s) 111, 802, 863, 1033, 1607, 1636
BtsI GCAGTG 1 cut(s) 1389
BtsIMutI CAGTG 1 cut(s) 1389
Cac8I GCNNGC 5 cut(s) 82, 489, 852, 1400, 1854
CaiI CAGNNNCTG 1 cut(s) 1808
CfoI GCGC 3 cut(s) 317, 379, 740
Cfr10I RCCGGY 2 cut(s) 583, 714
Cfr13I GGNCC 8 cut(s) 290, 702, 743, 758, 966, 1017, 1589, 1939
Cfr9I CCCGGG 1 cut(s) 760
ClaI ATCGAT 2 cut(s) 729, 1007
CseI GACGC 2 cut(s) 421, 1908
CsiI ACCWGGT 1 cut(s) 885
CspAI ACCGGT 1 cut(s) 714
DdeI CTNAG 7 cut(s) 85, 476, 1099, 1269, 1599, 1705, 1858
DinI GGCGCC 1 cut(s) 739
DpnI GATC 6 cut(s) 56, 95, 514, 655, 728, 1964
DpnII GATC 6 cut(s) 54, 93, 512, 653, 726, 1962
DraIII CACNNNGTG 2 cut(s) 684, 1718
DrdI GACNNNNNNGTC 1 cut(s) 1802
DseDI GACNNNNNNGTC 1 cut(s) 1802
EaeI YGGCCR 4 cut(s) 12, 24, 823, 1573
EagI CGGCCG 1 cut(s) 823
Eam1104I CTCTTC 2 cut(s) 244, 670
EarI CTCTTC 2 cut(s) 244, 670
EciI GGCGGA 2 cut(s) 269, 328
Ecl136II GAGCTC 1 cut(s) 487
EclXI CGGCCG 1 cut(s) 823
Eco130I CCWWGG 6 cut(s) 9, 21, 830, 867, 1532, 1952
Eco147I AGGCCT 1 cut(s) 1856
Eco24I GRGCYC 2 cut(s) 489, 1532
Eco31I GGTCTC 3 cut(s) 107, 840, 1459
Eco47I GGWCC 5 cut(s) 702, 743, 966, 1017, 1939
Eco52I CGGCCG 1 cut(s) 823
Eco53kI GAGCTC 1 cut(s) 487
Eco88I CYCGRG 2 cut(s) 760, 985
EcoICRI GAGCTC 1 cut(s) 487
EcoO109I RGGNCCY 2 cut(s) 1017, 1939
EcoRI GAATTC 2 cut(s) 912, 931
EcoRII CCWGG 2 cut(s) 426, 885
EcoT14I CCWWGG 6 cut(s) 9, 21, 830, 867, 1532, 1952
EcoT38I GRGCYC 2 cut(s) 489, 1532
EgeI GGCGCC 1 cut(s) 739
EheI GGCGCC 1 cut(s) 739
ErhI CCWWGG 6 cut(s) 9, 21, 830, 867, 1532, 1952
Esp3I CGTCTC 1 cut(s) 439
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 2 cut(s) 674, 1906
FauI CCCGC 1 cut(s) 137
FauNDI CATATG 1 cut(s) 1518
FblI GTMKAC 1 cut(s) 1148
Fnu4HI GCNGC 8 cut(s) 27, 66, 342, 518, 694, 813, 826, 1635
FokI GGATG 6 cut(s) 98, 789, 850, 1040, 1594, 1643
FriOI GRGCYC 2 cut(s) 489, 1532
Fsp4HI GCNGC 8 cut(s) 27, 66, 342, 518, 694, 813, 826, 1635
FspBI CTAG 1 cut(s) 1533
GlaI GCGC 3 cut(s) 316, 378, 739
GluI GCNGC 8 cut(s) 27, 66, 342, 518, 694, 813, 826, 1635
GsuI CTGGAG 3 cut(s) 289, 295, 1546
HaeII RGCGCY 2 cut(s) 318, 741
HaeIII GGCC 8 cut(s) 14, 26, 291, 759, 825, 1575, 1591, 1856
HapII CCGG 5 cut(s) 584, 705, 715, 741, 761
HgaI GACGC 2 cut(s) 421, 1908
HhaI GCGC 3 cut(s) 317, 379, 740
Hin1I GRCGYC 3 cut(s) 432, 738, 1919
Hin6I GCGC 3 cut(s) 315, 377, 738
HinP1I GCGC 3 cut(s) 315, 377, 738
HincII GTYRAC 1 cut(s) 1748
HindII GTYRAC 1 cut(s) 1748
HinfI GANTC 9 cut(s) 155, 386, 539, 662, 983, 1202, 1232, 1579, 1700
HpaII CCGG 5 cut(s) 584, 705, 715, 741, 761
HphI GGTGA 6 cut(s) 109, 670, 785, 1102, 1340, 1500
Hpy166II GTNNAC 4 cut(s) 598, 1149, 1443, 1748
Hpy188III TCNNGA 9 cut(s) 52, 104, 571, 611, 724, 1078, 1525, 1697, 1831
Hpy8I GTNNAC 4 cut(s) 598, 1149, 1443, 1748
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 7 cut(s) 56, 169, 307, 340, 510, 568, 1621
HpyCH4III ACNGT 1 cut(s) 1408
HpyCH4IV ACGT 4 cut(s) 276, 369, 1752, 1911
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 138, 288, 851, 860, 1265, 1333, 1738
HpyF3I CTNAG 7 cut(s) 85, 476, 1099, 1269, 1599, 1705, 1858
HpySE526I ACGT 4 cut(s) 276, 369, 1752, 1911
Hsp92I GRCGYC 3 cut(s) 432, 738, 1919
HspAI GCGC 3 cut(s) 315, 377, 738
KasI GGCGCC 1 cut(s) 737
Kzo9I GATC 6 cut(s) 54, 93, 512, 653, 726, 1962
LmnI GCTCC 5 cut(s) 94, 314, 485, 1527, 1737
Lsp1109I GCAGC 2 cut(s) 705, 1621
LweI GCATC 5 cut(s) 411, 872, 1225, 1441, 1621
MabI ACCWGGT 1 cut(s) 885
MaeI CTAG 1 cut(s) 1533
MaeII ACGT 4 cut(s) 276, 369, 1752, 1911
MaeIII GTNAC 4 cut(s) 1094, 1424, 1506, 1815
MalI GATC 6 cut(s) 56, 95, 514, 655, 728, 1964
MbiI CCGCTC 1 cut(s) 520
MboI GATC 6 cut(s) 54, 93, 512, 653, 726, 1962
MboII GAAGA 6 cut(s) 195, 231, 657, 884, 1283, 1994
MflI RGATCY 2 cut(s) 653, 1962
MhlI GDGCHC 3 cut(s) 489, 1400, 1532
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 7 cut(s) 646, 797, 912, 931, 1482, 1666, 2045
MluNI TGGCCA 1 cut(s) 14
Mly113I GGCGCC 1 cut(s) 738
MlyI GAGTC 6 cut(s) 149, 533, 656, 977, 1196, 1573
MmeI TCCRAC 1 cut(s) 381
Mox20I TGGCCA 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 1778
MscI TGGCCA 1 cut(s) 14
MseI TTAA 1 cut(s) 1140
MslI CAYNNNNRTG 2 cut(s) 20, 960
Msp20I TGGCCA 1 cut(s) 14
MspI CCGG 5 cut(s) 584, 705, 715, 741, 761
MspR9I CCNGG 5 cut(s) 428, 706, 761, 762, 887
Mva1269I GAATGC 1 cut(s) 674
MvaI CCWGG 2 cut(s) 428, 887
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 8 cut(s) 86, 138, 288, 851, 860, 1265, 1333, 1738
NarI GGCGCC 1 cut(s) 738
NciI CCSGG 3 cut(s) 706, 761, 762
NcoI CCATGG 3 cut(s) 9, 21, 1952
NdeI CATATG 1 cut(s) 1518
NdeII GATC 6 cut(s) 54, 93, 512, 653, 726, 1962
NlaIV GGNNCC 5 cut(s) 292, 739, 967, 1529, 1941
NmuCI GTSAC 2 cut(s) 1506, 1815
NspI RCATGY 3 cut(s) 959, 1327, 1498
NspV TTCGAA 1 cut(s) 2029
PaeR7I CTCGAG 1 cut(s) 985
PceI AGGCCT 1 cut(s) 1856
PciI ACATGT 1 cut(s) 955
PcsI WCGNNNNNNNCGW 4 cut(s) 282, 381, 543, 1151
PctI GAATGC 1 cut(s) 674
PdmI GAANNNNTTC 1 cut(s) 1778
PfeI GAWTC 3 cut(s) 386, 1232, 1700
PflMI CCANNNNNTGG 1 cut(s) 1912
PinAI ACCGGT 1 cut(s) 714
PkrI GCNGC 8 cut(s) 28, 67, 343, 519, 695, 814, 827, 1636
Ple19I CGATCG 1 cut(s) 515
PleI GAGTC 6 cut(s) 149, 533, 656, 977, 1196, 1573
PluTI GGCGCC 1 cut(s) 741
PpsI GAGTC 6 cut(s) 149, 533, 656, 977, 1196, 1573
PpuMI RGGWCCY 2 cut(s) 1017, 1939
PscI ACATGT 1 cut(s) 955
Psp124BI GAGCTC 1 cut(s) 489
Psp5II RGGWCCY 2 cut(s) 1017, 1939
Psp6I CCWGG 2 cut(s) 426, 885
PspGI CCWGG 2 cut(s) 426, 885
PspN4I GGNNCC 5 cut(s) 292, 739, 967, 1529, 1941
PspPI GGNCC 8 cut(s) 290, 702, 743, 758, 966, 1017, 1589, 1939
PspPPI RGGWCCY 2 cut(s) 1017, 1939
PspXI VCTCGAGB 1 cut(s) 985
PstI CTGCAG 1 cut(s) 1491
PstNI CAGNNNCTG 1 cut(s) 1808
PsuI RGATCY 2 cut(s) 653, 1962
PvuI CGATCG 1 cut(s) 515
RseI CAYNNNNRTG 2 cut(s) 20, 960
SacI GAGCTC 1 cut(s) 489
SaqAI TTAA 1 cut(s) 1140
SatI GCNGC 8 cut(s) 27, 66, 342, 518, 694, 813, 826, 1635
Sau3AI GATC 6 cut(s) 54, 93, 512, 653, 726, 1962
Sau96I GGNCC 8 cut(s) 290, 702, 743, 758, 966, 1017, 1589, 1939
SchI GAGTC 6 cut(s) 149, 533, 656, 977, 1196, 1573
ScrFI CCNGG 5 cut(s) 428, 706, 761, 762, 887
SduI GDGCHC 3 cut(s) 489, 1400, 1532
SexAI ACCWGGT 1 cut(s) 885
SfaNI GCATC 5 cut(s) 411, 872, 1225, 1441, 1621
SfcI CTRYAG 2 cut(s) 1487, 1686
SfoI GGCGCC 1 cut(s) 739
Sfr274I CTCGAG 1 cut(s) 985
SfuI TTCGAA 1 cut(s) 2029
SinI GGWCC 5 cut(s) 702, 743, 966, 1017, 1939
SlaI CTCGAG 1 cut(s) 985
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 2 cut(s) 20, 960
SmlI CTYRAG 3 cut(s) 985, 1460, 1736
SmoI CTYRAG 3 cut(s) 985, 1460, 1736
Sse9I AATT 7 cut(s) 646, 797, 912, 931, 1482, 1666, 2045
SseBI AGGCCT 1 cut(s) 1856
SspDI GGCGCC 1 cut(s) 737
SspI AATATT 1 cut(s) 1224
SspMI CTAG 1 cut(s) 1533
SstI GAGCTC 1 cut(s) 489
StuI AGGCCT 1 cut(s) 1856
StyD4I CCNGG 5 cut(s) 426, 704, 759, 760, 885
StyI CCWWGG 6 cut(s) 9, 21, 830, 867, 1532, 1952
TaaI ACNGT 1 cut(s) 1408
TaiI ACGT 4 cut(s) 279, 372, 1755, 1914
TaqII GACCGA 1 cut(s) 89
TasI AATT 7 cut(s) 646, 797, 912, 931, 1482, 1666, 2045
TauI GCSGC 6 cut(s) 29, 68, 344, 520, 815, 828
TfiI GAWTC 3 cut(s) 386, 1232, 1700
Tru1I TTAA 1 cut(s) 1140
Tru9I TTAA 1 cut(s) 1140
TscAI CASTG 1 cut(s) 1389
TseFI GTSAC 2 cut(s) 1506, 1815
TseI GCWGC 2 cut(s) 693, 1634
Tsp45I GTSAC 2 cut(s) 1506, 1815
TspDTI ATGAA 8 cut(s) 578, 791, 924, 1107, 1596, 1679, 1750, 1994
TspGWI ACGGA 2 cut(s) 666, 795
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 1 cut(s) 1389
Van91I CCANNNNNTGG 1 cut(s) 1912
VpaK11BI GGWCC 5 cut(s) 702, 743, 966, 1017, 1939
XapI RAATTY 3 cut(s) 797, 912, 931
XceI RCATGY 3 cut(s) 959, 1327, 1498
XcmI CCANNNNNNNNNTGG 3 cut(s) 1583, 1909, 1950
XhoI CTCGAG 1 cut(s) 985
XmaI CCCGGG 1 cut(s) 760
XmaJI CCTAGG 1 cut(s) 1532
XmiI GTMKAC 1 cut(s) 1148
XmnI GAANNNNTTC 1 cut(s) 1778
XspI CTAG 1 cut(s) 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.