MD17G1047800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012436
Physical Location & Seq
Reverse (-)
3469970 .. 3472059
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1047800.v1.1.491

Sequence Viewer

Length: 2031 bp
ATGGCCACTACCACCACTCTCCTCCTTCTCCTGATCCTAACCGCCGCCATAACCACCGCCACCTCAGCCCCGATCCTAGGTCTCGACACCTTCCTCACCCACCAATCCCGCCTTGACCGCCATGCCACCAACGACTCCTTCCTCTCTCTCTCCTCCACCCTCAAAAACTCCCTTTTCCATTCTACCCCTCTCTCCCACACCCCCCACTCCCTCATCTCCTCTCTCCTCTCCCTCTCCCTCCCCGTCTCTCTCCACGTCCGCCTTGTCGGCCCCTCCTTCCCCCCCAGCTCCGCCGCCCTCCTCTCCTCCTTCCTCACCGCCGCCTCCCAACCCTCCAACCACTTCCACGTTATCGCCCCCTACGAATCCTTCTCTCACCGCCTTGCCGTCCGGCACTCTATCCACCTCGACGTTTCTCACTCCCCCTCTCTCGCATCCCTCCTCACCAAAACCCTAAGCTCCGAGCTCGCCAAAACCACCTCCAGCCTCCGATCGCCGCTCCTCTCCGTCCCCTACGACTTAATCGACCAAATCATCAAGCAGGACTTCGAGAAAGAGAAGCCGGTTCATGGCGTTTACTTGTATTTCCTCGATATGGGTAAGCAATCCAAACCCTATGCATACAATTACGGATCTGGGGACTCTTCCCCTGCATTCACCAAGTGTTTGGGCAGCATTTGGACCGGGAAAGACCGGTATATCTGGATCGATTTGGGCGCCGGTCCGGTCGATTACGGCCCGGCGCTTTCAGGTGATGGGGTTCTGCCCAGAGGCGAGTTTCATCCTCTGGCGGCGTTGCACGGCCGCCCCAAGGCTCAAAAGGCGCTGCTAGCTGACTTGGCTTCCTTGGTTTGGAGTGCTTACCAGGTTCTTCTCGTGCCCTCCTTGAGAATTCCTGTTCAGTTCGAGAATTCATTGATTGTTCAGTTCATACATGTTTATGGTTCCGATGGTGGTAAGGATTCGAGTGGTTTGGACTGGAAATTGATTGAGAGGACGTTTATGGATGAGGCAAATGGTAATGGTTTGTTGTTAGGGGATCAGACTTTGAGTTTCAGGACTAGTCGGGTGAGTTACTCAGACTGCCTGATTTGTTCATTCGCGGTTTCGAGGTCTACTAACTCGTATACATCGAGGTTCTTGTTTGATAACTATACTTTGATTGCAAGTGAGTATTTGGACTCGAAGCGATTGCATCAGATATTGTCTGATTCGGCTGAGGAGTTTAGGAGAGTGGCTGGGCTCCCTGAGGAGGATTTTGCTAGGGTTCTTCCTGTTTATGTGTTTGATTTGGACTACAACATGCTGTTGTTGCTCGATAGGTATCACCAATCAGTTGCTTTTAAAGACATGGTTATTGCAGTGAGGACGAAGAACACGCAGACTGTGAGTGATTATAACTGTAATGGGCGTCATGTGTTTACGCAAACAAGAGAGCTTGAGAGACCACTTGTTGGTTCGATTTTGCAGAGCATGTGGGGAGTGTCACCGACCCATATGCTGTGGAGCCCTAGGCACAATACTACATTGGTGGATTACACATGGAGTATTGGCCAGACTCCCTTTGGGCCGTTCTCTGAGATTTCATCCTTATCATTCGTACAGAAGGATGCTGCTCGGAGAAATGTTCTTTTGACATCTCTGAATTACACCATATCGAGTGCTGTAGATGTTCTCCAATCCATAGCTGCACATGGCGGGGAAAGAAAGCTCCTTAAGCGTAGTCGACACGTTGAATTCATACAGAGGTGGAACTTGTTCAGGTACAAGCTGGACAAAGCAGTCTCTGCAATGTCGCTTTTGGATTTTGGGATGGCTTTGTTTTATTTGCGGTCCTCAGACCACGACCTATACGCTATCCACTCTCTCGTCTATCAAGCCTCCCAAGAAGTGGAGGTATCCCTTGTATGTTTCAAAGATCCACCAGTTCCGTGGAATGGGATCTTCATGGTGGGATTTGTTATCGCTGTTGCCTTTTATCTTCATTCTAAACGAGATAAAGTCTTCAGAAATAAACGTAAGCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

677

Amino Acids

75.55

Weight (kDa)

8.68

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 81 - 294 8e-87 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1398
AasI GACNNNNNNGTC 1 cut(s) 1781
AccB1I GGYRCC 1 cut(s) 716
AccB7I CCANNNNNTGG 2 cut(s) 1454, 1891
AccBSI CCGCTC 1 cut(s) 499
AccI GTMKAC 3 cut(s) 1115, 1127, 1726
AccII CGCG 1 cut(s) 1103
AclWI GGATC 7 cut(s) 28, 67, 640, 713, 1047, 1913, 1949
AcoI YGGCCR 3 cut(s) 3, 802, 1552
AcsI RAATTY 3 cut(s) 891, 910, 1736
AcuI CTGAAG 1 cut(s) 1990
AcyI GRCGYC 2 cut(s) 717, 1411
AdeI CACNNNGTG 1 cut(s) 663
AfaI GTAC 2 cut(s) 1602, 1766
AfiI CCNNNNNNNGG 9 cut(s) 77, 569, 595, 811, 852, 1252, 1454, 1891, 1937
AflII CTTAAG 1 cut(s) 1715
AflIII ACRYGT 2 cut(s) 934, 1729
AgeI ACCGGT 1 cut(s) 693
AgsI TTSAA 2 cut(s) 1736, 1915
AhlI ACTAGT 1 cut(s) 1061
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 1 cut(s) 864
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 8 cut(s) 288, 459, 466, 833, 1438, 1688, 1711, 1771
AluI AGCT 8 cut(s) 288, 459, 466, 833, 1438, 1688, 1711, 1771
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 4 cut(s) 86, 250, 1438, 1789
AlwI GGATC 7 cut(s) 28, 67, 640, 713, 1047, 1913, 1949
AlwNI CAGNNNCTG 1 cut(s) 1787
AoxI GGCC 6 cut(s) 3, 268, 736, 802, 1552, 1568
ApeKI GCWGC 4 cut(s) 672, 826, 1613, 1688
ApoI RAATTY 3 cut(s) 891, 910, 1736
AsiGI ACCGGT 1 cut(s) 693
Asp700I GAANNNNTTC 1 cut(s) 1757
AspA2I CCTAGG 2 cut(s) 76, 1511
AspLEI GCGC 3 cut(s) 719, 745, 826
AspS9I GGNCC 6 cut(s) 269, 681, 722, 737, 1568, 1833
AsuC2I CCSGG 2 cut(s) 685, 740
AsuHPI GGTGA 9 cut(s) 88, 307, 368, 436, 649, 764, 1081, 1319, 1479
AsuNHI GCTAGC 1 cut(s) 829
AvaII GGWCC 3 cut(s) 681, 722, 1833
AvrII CCTAGG 2 cut(s) 76, 1511
AxyI CCTNAGG 1 cut(s) 1248
BaeGI GKGCMC 1 cut(s) 882
BalI TGGCCA 2 cut(s) 5, 1554
BanI GGYRCC 1 cut(s) 716
BanII GRGCYC 3 cut(s) 468, 1245, 1511
BauI CACGAG 1 cut(s) 875
BbsI GAAGAC 1 cut(s) 1996
Bbv12I GWGCWC 1 cut(s) 468
BbvCI CCTCAGC 2 cut(s) 64, 1218
BbvI GCAGC 4 cut(s) 684, 813, 1600, 1675
BccI CCATC 3 cut(s) 749, 944, 1807
BceAI ACGGC 4 cut(s) 371, 751, 817, 1555
BcgI CGANNNNNNTGC 4 cut(s) 1174, 1208, 1480, 1514
BciT130I CCWGG 1 cut(s) 866
BciVI GTATCC 1 cut(s) 1909
BcnI CCSGG 2 cut(s) 685, 740
BcoDI GTCTC 4 cut(s) 86, 250, 1438, 1789
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 5 cut(s) 77, 830, 1062, 1263, 1512
BfmI CTRYAG 1 cut(s) 1665
BfoI RGCGCY 3 cut(s) 720, 746, 827
BfrI CTTAAG 1 cut(s) 1715
BfuI GTATCC 1 cut(s) 1909
BglI GCCNNNNNGGC 1 cut(s) 267
BlnI CCTAGG 2 cut(s) 76, 1511
Bme1390I CCNGG 3 cut(s) 685, 740, 866
Bme18I GGWCC 3 cut(s) 681, 722, 1833
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 6 cut(s) 269, 681, 722, 737, 1568, 1833
BmiI GGNNCC 5 cut(s) 271, 718, 946, 1244, 1508
BmrFI CCNGG 3 cut(s) 685, 740, 866
BmsI GCATC 3 cut(s) 443, 1204, 1600
BmtI GCTAGC 1 cut(s) 833
BpiI GAAGAC 1 cut(s) 1996
BpmI CTGGAG 1 cut(s) 466
Bpu10I CCTNAGC 3 cut(s) 64, 455, 1218
BpuEI CTTGAG 2 cut(s) 907, 1460
BpuMI CCSGG 2 cut(s) 685, 740
Bsa29I ATCGAT 1 cut(s) 708
BsaBI GATNNNNATC 1 cut(s) 1323
BsaHI GRCGYC 2 cut(s) 717, 1411
BsaI GGTCTC 2 cut(s) 86, 1438
BsaJI CCNNGG 5 cut(s) 76, 810, 846, 1511, 1931
BsaWI WCCGGW 2 cut(s) 693, 724
Bsc4I CCNNNNNNNGG 9 cut(s) 77, 569, 595, 811, 852, 1252, 1454, 1891, 1937
Bse118I RCCGGY 3 cut(s) 562, 693, 719
Bse1I ACTGG 2 cut(s) 983, 1925
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 1 cut(s) 1797
Bse8I GATNNNNATC 1 cut(s) 1323
BseBI CCWGG 1 cut(s) 866
BseCI ATCGAT 1 cut(s) 708
BseDI CCNNGG 5 cut(s) 76, 810, 846, 1511, 1931
BseGI GGATG 6 cut(s) 434, 781, 1012, 1586, 1615, 1818
BseJI GATNNNNATC 1 cut(s) 1323
BseLI CCNNNNNNNGG 9 cut(s) 77, 569, 595, 811, 852, 1252, 1454, 1891, 1937
BseMI GCAATG 1 cut(s) 1797
BseMII CTCAG 6 cut(s) 78, 1092, 1209, 1239, 1569, 1851
BseNI ACTGG 2 cut(s) 983, 1925
BseSI GKGCMC 1 cut(s) 882
BseX3I CGGCCG 1 cut(s) 802
BseXI GCAGC 4 cut(s) 684, 813, 1600, 1675
BseYI CCCAGC 2 cut(s) 284, 1238
BsgI GTGCAG 1 cut(s) 1674
Bsh1236I CGCG 1 cut(s) 1103
Bsh1285I CGRYCG 3 cut(s) 494, 729, 805
BshFI GGCC 6 cut(s) 5, 270, 738, 804, 1554, 1570
BshNI GGYRCC 1 cut(s) 716
BshTI ACCGGT 1 cut(s) 693
BshVI ATCGAT 1 cut(s) 708
BsiEI CGRYCG 3 cut(s) 494, 729, 805
BsiHKAI GWGCWC 1 cut(s) 468
BsiSI CCGG 7 cut(s) 391, 563, 684, 694, 720, 725, 740
BslFI GGGAC 2 cut(s) 494, 653
BslI CCNNNNNNNGG 9 cut(s) 77, 569, 595, 811, 852, 1252, 1454, 1891, 1937
BsmAI GTCTC 4 cut(s) 86, 250, 1438, 1789
BsmBI CGTCTC 1 cut(s) 250
BsmFI GGGAC 2 cut(s) 494, 653
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 6 cut(s) 5, 270, 738, 804, 1554, 1570
Bso31I GGTCTC 2 cut(s) 86, 1438
Bsp1286I GDGCHC 4 cut(s) 468, 882, 1245, 1511
Bsp143I GATC 8 cut(s) 33, 72, 491, 632, 705, 1039, 1918, 1941
BspANI GGCC 6 cut(s) 5, 270, 738, 804, 1554, 1570
BspCNI CTCAG 6 cut(s) 77, 1091, 1210, 1240, 1570, 1850
BspDI ATCGAT 1 cut(s) 708
BspFNI CGCG 1 cut(s) 1103
BspLI GGNNCC 5 cut(s) 271, 718, 946, 1244, 1508
BspOI GCTAGC 1 cut(s) 833
BspPI GGATC 7 cut(s) 28, 67, 640, 713, 1047, 1913, 1949
BspT107I GGYRCC 1 cut(s) 716
BspTI CTTAAG 1 cut(s) 1715
BspTNI GGTCTC 2 cut(s) 86, 1438
BsrBI CCGCTC 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 1797
BsrFI RCCGGY 3 cut(s) 562, 693, 719
BsrI ACTGG 2 cut(s) 983, 1925
BssAI RCCGGY 3 cut(s) 562, 693, 719
BssECI CCNNGG 5 cut(s) 76, 810, 846, 1511, 1931
BssMI GATC 8 cut(s) 33, 72, 491, 632, 705, 1039, 1918, 1941
BssNAI GTATAC 1 cut(s) 1128
BssNI GRCGYC 2 cut(s) 717, 1411
BssSI CACGAG 1 cut(s) 875
BssT1I CCWWGG 4 cut(s) 76, 810, 846, 1511
Bst1107I GTATAC 1 cut(s) 1128
Bst2BI CACGAG 1 cut(s) 875
Bst2UI CCWGG 1 cut(s) 866
Bst4CI ACNGT 2 cut(s) 1387, 1403
Bst6I CTCTTC 1 cut(s) 649
BstACI GRCGYC 2 cut(s) 717, 1411
BstAFI CTTAAG 1 cut(s) 1715
BstAPI GCANNNNNTGC 1 cut(s) 1787
BstC8I GCNNGC 2 cut(s) 468, 831
BstDEI CTNAG 7 cut(s) 64, 455, 1078, 1218, 1248, 1578, 1837
BstDSI CCRYGG 1 cut(s) 1931
BstF5I GGATG 6 cut(s) 434, 781, 1012, 1586, 1615, 1818
BstFNI CGCG 1 cut(s) 1103
BstH2I RGCGCY 3 cut(s) 720, 746, 827
BstHHI GCGC 3 cut(s) 719, 745, 826
BstKTI GATC 8 cut(s) 36, 75, 494, 635, 708, 1042, 1921, 1944
BstMAI GTCTC 4 cut(s) 86, 250, 1438, 1789
BstMBI GATC 8 cut(s) 33, 72, 491, 632, 705, 1039, 1918, 1941
BstMCI CGRYCG 3 cut(s) 494, 729, 805
BstMWI GCNNNNNNNGC 9 cut(s) 65, 117, 267, 821, 830, 839, 1312, 1717, 1787
BstNI CCWGG 1 cut(s) 866
BstNSI RCATGY 3 cut(s) 938, 1306, 1477
BstSCI CCNGG 3 cut(s) 683, 738, 864
BstSFI CTRYAG 1 cut(s) 1665
BstSLI GKGCMC 1 cut(s) 882
BstUI CGCG 1 cut(s) 1103
BstV1I GCAGC 4 cut(s) 684, 813, 1600, 1675
BstV2I GAAGAC 1 cut(s) 1996
BstX2I RGATCY 3 cut(s) 632, 1918, 1941
BstXI CCANNNNNNTGG 2 cut(s) 667, 1932
BstYI RGATCY 3 cut(s) 632, 1918, 1941
BstZ17I GTATAC 1 cut(s) 1128
BstZI CGGCCG 1 cut(s) 802
Bsu15I ATCGAT 1 cut(s) 708
Bsu36I CCTNAGG 1 cut(s) 1248
BsuI GTATCC 1 cut(s) 1909
BsuRI GGCC 6 cut(s) 5, 270, 738, 804, 1554, 1570
BsuTUI ATCGAT 1 cut(s) 708
BtgI CCRYGG 1 cut(s) 1931
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 6 cut(s) 434, 781, 1012, 1586, 1615, 1818
BtsI GCAGTG 1 cut(s) 1368
BtsIMutI CAGTG 1 cut(s) 1368
Cac8I GCNNGC 2 cut(s) 468, 831
CaiI CAGNNNCTG 1 cut(s) 1787
CfoI GCGC 3 cut(s) 719, 745, 826
Cfr10I RCCGGY 3 cut(s) 562, 693, 719
Cfr13I GGNCC 6 cut(s) 269, 681, 722, 737, 1568, 1833
ClaI ATCGAT 1 cut(s) 708
CpoI CGGWCCG 1 cut(s) 722
CseI GACGC 1 cut(s) 1400
CsiI ACCWGGT 1 cut(s) 864
Csp6I GTAC 2 cut(s) 1601, 1765
CspAI ACCGGT 1 cut(s) 693
CspI CGGWCCG 1 cut(s) 722
CviQI GTAC 2 cut(s) 1601, 1765
DdeI CTNAG 7 cut(s) 64, 455, 1078, 1218, 1248, 1578, 1837
DinI GGCGCC 1 cut(s) 718
DpnI GATC 8 cut(s) 35, 74, 493, 634, 707, 1041, 1920, 1943
DpnII GATC 8 cut(s) 33, 72, 491, 632, 705, 1039, 1918, 1941
DraI TTTAAA 1 cut(s) 1345
DraIII CACNNNGTG 1 cut(s) 663
DrdI GACNNNNNNGTC 1 cut(s) 1781
DseDI GACNNNNNNGTC 1 cut(s) 1781
EaeI YGGCCR 3 cut(s) 3, 802, 1552
EagI CGGCCG 1 cut(s) 802
Eam1104I CTCTTC 1 cut(s) 649
EarI CTCTTC 1 cut(s) 649
EciI GGCGGA 2 cut(s) 248, 280
Ecl136II GAGCTC 1 cut(s) 466
EclXI CGGCCG 1 cut(s) 802
Eco130I CCWWGG 4 cut(s) 76, 810, 846, 1511
Eco24I GRGCYC 3 cut(s) 468, 1245, 1511
Eco31I GGTCTC 2 cut(s) 86, 1438
Eco47I GGWCC 3 cut(s) 681, 722, 1833
Eco52I CGGCCG 1 cut(s) 802
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 1990
Eco81I CCTNAGG 1 cut(s) 1248
EcoICRI GAGCTC 1 cut(s) 466
EcoRI GAATTC 3 cut(s) 891, 910, 1736
EcoRII CCWGG 1 cut(s) 864
EcoT14I CCWWGG 4 cut(s) 76, 810, 846, 1511
EcoT22I ATGCAT 1 cut(s) 622
EcoT38I GRGCYC 3 cut(s) 468, 1245, 1511
EgeI GGCGCC 1 cut(s) 718
EheI GGCGCC 1 cut(s) 718
ErhI CCWWGG 4 cut(s) 76, 810, 846, 1511
Esp3I CGTCTC 1 cut(s) 250
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FaqI GGGAC 2 cut(s) 494, 653
FauI CCCGC 2 cut(s) 116, 1691
FauNDI CATATG 1 cut(s) 1497
FblI GTMKAC 3 cut(s) 1115, 1127, 1726
FokI GGATG 6 cut(s) 421, 768, 1019, 1573, 1622, 1825
FriOI GRGCYC 3 cut(s) 468, 1245, 1511
FspBI CTAG 5 cut(s) 77, 830, 1062, 1263, 1512
GlaI GCGC 3 cut(s) 718, 744, 825
GsaI CCCAGC 2 cut(s) 288, 1242
GsuI CTGGAG 1 cut(s) 466
HaeII RGCGCY 3 cut(s) 720, 746, 827
HaeIII GGCC 6 cut(s) 5, 270, 738, 804, 1554, 1570
HapII CCGG 7 cut(s) 391, 563, 684, 694, 720, 725, 740
HgaI GACGC 1 cut(s) 1400
HhaI GCGC 3 cut(s) 719, 745, 826
Hin1I GRCGYC 2 cut(s) 717, 1411
Hin6I GCGC 3 cut(s) 717, 743, 824
HinP1I GCGC 3 cut(s) 717, 743, 824
HincII GTYRAC 1 cut(s) 1727
HindII GTYRAC 1 cut(s) 1727
HinfI GANTC 7 cut(s) 134, 365, 641, 962, 1181, 1211, 1558
HpaII CCGG 7 cut(s) 391, 563, 684, 694, 720, 725, 740
HphI GGTGA 9 cut(s) 88, 307, 368, 436, 649, 764, 1081, 1319, 1479
Hpy166II GTNNAC 5 cut(s) 577, 1116, 1128, 1422, 1727
Hpy188III TCNNGA 6 cut(s) 31, 83, 550, 703, 907, 1057
Hpy8I GTNNAC 5 cut(s) 577, 1116, 1128, 1422, 1727
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 7 cut(s) 35, 100, 148, 286, 319, 379, 1600
HpyCH4III ACNGT 2 cut(s) 1387, 1403
HpyCH4IV ACGT 6 cut(s) 255, 348, 411, 998, 1731, 2017
HpyCH4V TGCA 9 cut(s) 620, 653, 799, 1166, 1195, 1361, 1468, 1691, 1790
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 117, 267, 821, 830, 839, 1312, 1717, 1787
HpyF3I CTNAG 7 cut(s) 64, 455, 1078, 1218, 1248, 1578, 1837
HpySE526I ACGT 6 cut(s) 255, 348, 411, 998, 1731, 2017
Hsp92I GRCGYC 2 cut(s) 717, 1411
HspAI GCGC 3 cut(s) 717, 743, 824
KasI GGCGCC 1 cut(s) 716
Kzo9I GATC 8 cut(s) 33, 72, 491, 632, 705, 1039, 1918, 1941
LmnI GCTCC 6 cut(s) 293, 464, 504, 1248, 1506, 1716
Lsp1109I GCAGC 4 cut(s) 684, 813, 1600, 1675
LweI GCATC 3 cut(s) 443, 1204, 1600
MabI ACCWGGT 1 cut(s) 864
MaeI CTAG 5 cut(s) 77, 830, 1062, 1263, 1512
MaeII ACGT 6 cut(s) 255, 348, 411, 998, 1731, 2017
MaeIII GTNAC 2 cut(s) 1073, 1485
MalI GATC 8 cut(s) 35, 74, 493, 634, 707, 1041, 1920, 1943
MbiI CCGCTC 1 cut(s) 499
MboI GATC 8 cut(s) 33, 72, 491, 632, 705, 1039, 1918, 1941
MboII GAAGA 7 cut(s) 636, 863, 1262, 1384, 1936, 1973, 1996
MflI RGATCY 3 cut(s) 632, 1918, 1941
MhlI GDGCHC 4 cut(s) 468, 882, 1245, 1511
MlsI TGGCCA 2 cut(s) 5, 1554
MluCI AATT 7 cut(s) 625, 891, 910, 983, 1645, 1736, 2024
MluNI TGGCCA 2 cut(s) 5, 1554
Mly113I GGCGCC 1 cut(s) 717
MlyI GAGTC 4 cut(s) 128, 635, 1175, 1552
MmeI TCCRAC 1 cut(s) 360
Mox20I TGGCCA 2 cut(s) 5, 1554
Mph1103I ATGCAT 1 cut(s) 622
MroXI GAANNNNTTC 1 cut(s) 1757
MscI TGGCCA 2 cut(s) 5, 1554
MseI TTAA 3 cut(s) 521, 1344, 1716
MslI CAYNNNNRTG 1 cut(s) 939
Msp20I TGGCCA 2 cut(s) 5, 1554
MspCI CTTAAG 1 cut(s) 1715
MspI CCGG 7 cut(s) 391, 563, 684, 694, 720, 725, 740
MspR9I CCNGG 3 cut(s) 685, 740, 866
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 1 cut(s) 866
MvnI CGCG 1 cut(s) 1103
MwoI GCNNNNNNNGC 9 cut(s) 65, 117, 267, 821, 830, 839, 1312, 1717, 1787
NarI GGCGCC 1 cut(s) 717
NciI CCSGG 2 cut(s) 685, 740
NdeI CATATG 1 cut(s) 1497
NdeII GATC 8 cut(s) 33, 72, 491, 632, 705, 1039, 1918, 1941
NheI GCTAGC 1 cut(s) 829
NlaIV GGNNCC 5 cut(s) 271, 718, 946, 1244, 1508
NmuCI GTSAC 1 cut(s) 1485
NsiI ATGCAT 1 cut(s) 622
NspI RCATGY 3 cut(s) 938, 1306, 1477
PciI ACATGT 1 cut(s) 934
PcsI WCGNNNNNNNCGW 3 cut(s) 360, 522, 1130
PctI GAATGC 1 cut(s) 653
PdmI GAANNNNTTC 1 cut(s) 1757
PfeI GAWTC 3 cut(s) 365, 962, 1211
PflMI CCANNNNNTGG 2 cut(s) 1454, 1891
PinAI ACCGGT 1 cut(s) 693
Ple19I CGATCG 1 cut(s) 494
PleI GAGTC 4 cut(s) 128, 635, 1175, 1552
PluTI GGCGCC 1 cut(s) 720
PpsI GAGTC 4 cut(s) 128, 635, 1175, 1552
PscI ACATGT 1 cut(s) 934
PsiI TTATAA 1 cut(s) 1398
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 864
PspFI CCCAGC 2 cut(s) 284, 1238
PspGI CCWGG 1 cut(s) 864
PspN4I GGNNCC 5 cut(s) 271, 718, 946, 1244, 1508
PspPI GGNCC 6 cut(s) 269, 681, 722, 737, 1568, 1833
PstNI CAGNNNCTG 1 cut(s) 1787
PsuI RGATCY 3 cut(s) 632, 1918, 1941
PvuI CGATCG 1 cut(s) 494
RsaI GTAC 2 cut(s) 1602, 1766
RsaNI GTAC 2 cut(s) 1601, 1765
RseI CAYNNNNRTG 1 cut(s) 939
Rsr2I CGGWCCG 1 cut(s) 722
RsrII CGGWCCG 1 cut(s) 722
SacI GAGCTC 1 cut(s) 468
SalI GTCGAC 1 cut(s) 1725
SaqAI TTAA 3 cut(s) 521, 1344, 1716
Sau3AI GATC 8 cut(s) 33, 72, 491, 632, 705, 1039, 1918, 1941
Sau96I GGNCC 6 cut(s) 269, 681, 722, 737, 1568, 1833
SchI GAGTC 4 cut(s) 128, 635, 1175, 1552
ScrFI CCNGG 3 cut(s) 685, 740, 866
SduI GDGCHC 4 cut(s) 468, 882, 1245, 1511
SexAI ACCWGGT 1 cut(s) 864
SfaNI GCATC 3 cut(s) 443, 1204, 1600
SfcI CTRYAG 1 cut(s) 1665
SfoI GGCGCC 1 cut(s) 718
SinI GGWCC 3 cut(s) 681, 722, 1833
SmiMI CAYNNNNRTG 1 cut(s) 939
SmlI CTYRAG 3 cut(s) 886, 1439, 1715
SmoI CTYRAG 3 cut(s) 886, 1439, 1715
SpeI ACTAGT 1 cut(s) 1061
Sse9I AATT 7 cut(s) 625, 891, 910, 983, 1645, 1736, 2024
SspDI GGCGCC 1 cut(s) 716
SspMI CTAG 5 cut(s) 77, 830, 1062, 1263, 1512
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 3 cut(s) 683, 738, 864
StyI CCWWGG 4 cut(s) 76, 810, 846, 1511
TaaI ACNGT 2 cut(s) 1387, 1403
TaiI ACGT 6 cut(s) 258, 351, 414, 1001, 1734, 2020
TasI AATT 7 cut(s) 625, 891, 910, 983, 1645, 1736, 2024
TauI GCSGC 6 cut(s) 47, 296, 323, 499, 794, 807
TfiI GAWTC 3 cut(s) 365, 962, 1211
Tru1I TTAA 3 cut(s) 521, 1344, 1716
Tru9I TTAA 3 cut(s) 521, 1344, 1716
TscAI CASTG 1 cut(s) 1368
TseFI GTSAC 1 cut(s) 1485
TseI GCWGC 4 cut(s) 672, 826, 1613, 1688
Tsp45I GTSAC 1 cut(s) 1485
TspDTI ATGAA 9 cut(s) 557, 770, 903, 919, 1086, 1575, 1729, 1936, 1973
TspGWI ACGGA 3 cut(s) 496, 645, 1920
TspRI CASTG 1 cut(s) 1368
Van91I CCANNNNNTGG 2 cut(s) 1454, 1891
Vha464I CTTAAG 1 cut(s) 1715
VpaK11BI GGWCC 3 cut(s) 681, 722, 1833
XapI RAATTY 3 cut(s) 891, 910, 1736
XceI RCATGY 3 cut(s) 938, 1306, 1477
XcmI CCANNNNNNNNNTGG 2 cut(s) 1562, 1929
XmaJI CCTAGG 2 cut(s) 76, 1511
XmiI GTMKAC 3 cut(s) 1115, 1127, 1726
XmnI GAANNNNTTC 1 cut(s) 1757
XspI CTAG 5 cut(s) 77, 830, 1062, 1263, 1512
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.