Rroxscaffold_2G00082500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012436
Physical Location & Seq
Reverse (-)
5301795 .. 5307268
5474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082500.1

Sequence Viewer

Length: 2082 bp
ATGGCGCTTTCCATCAGCAATGAGGTCAGAGCTGGAATAGATCCAGATCGTGTACGGAGAGTAGGCATGGCTGGTCGTTTGATGGAGAGAAGTGCCGATGCCAACTCCTCTGAATTCTTGATCGCTGGATTACAATCTGAGGCTCGAGCGTGTGCGTGCATTGACCCCACCGCTTTATCTGATCCTTTCACTGTTTTGGAGAATCGACTAAAGTACACAGAGGCACATTCCACTAGATTTGTTGAAACAATTGAAGAGATTCTGAGCATATACAGCTTCGATTCTCCACTTCTACTTTTTGTGTTCTTGGTTATAAGCAGGGGAGATGTATCGAGTTTGATGATGGTATGTATGATTGTAGAACTTAATTTTCTTGTCAATGTTATTGCTATGTTGGAAAAATTTAACAAGTCTCTTTTAAAAATACGAAATTTAATCAACCGAGGGAAAGTTGGGTTAGAGGTTTCTCTTTTCCCGACCAGAGTCCACAGTCACCTCTCCAAAACCCTAGCCTCCGAGCTCGCCAAAACCCCCGCCAGTCTCCGATCGCCGCTCGTCTCCGTCGCCTACGACTCAATCGACCGGATCATCAAGCAGGACTTCGAGAAAGAGAAGCCGGTCCACGGCGTTTACCTCTATTTCTTGGATTTGGGGCCCCAATCCAAGAGCTATGCTTACAGCTATGGATCTGGCGACTCCTCGCCTGGCTTCACCAAGTGTTTGGGGAGCGTTTGGACCGGGTCGGACCGCTACATTTGGATCGATTTGGGCGCCGGACCGGTCGACTACGGCCCGGCGCTTTCCGGCGACGGTGTCTTGCCGAAAGGCGAGTTTCATCCTCTGGCGGCGTTGCACGGCTCGCCGAAAGCTCAGAAGGCGTTGCTGGCGGACTTGGCTTCCTTGGTGTGGAGTGCTTACCAGGTTCTTCTTGTTCCCTCTCTGAGAATTCCTGTTCAATATGAGAATTCTTTGATTGTTCAGTTTATACATGTTTATGGAGGTGAGAGCGGTAAGGATTTGAGTGGTTTGGATTGGAAATCGATTGAGAGGACCTTTATGGATGAGGCTAAGGATAATGGCCTGTTGTTAGGGGATCAGTCTTTGAGTTTCAAGAATTATAAGGTGAAGTATTCGGAGTGCCCGATATGTTCATTTGCTATTGCCAGGTCGATTAATTCGTATACGTCGAGGTTCTTGTTTGATAACTATACGTTGATTGCGAGTGAGTATTTGGATTCGAAGAGATTGCACCAGATATTGTCTGACTCTGCTGAGGAGTTTAGGAGGGTGGCGGGGTTTCCTGAGGAGGATTTTGGCAGGGTGCTTCCTGTGTTTGTGTTTGATTTGGACTACCACATGCTGTTGCTGCTCGATAGGTATCACCAAACTGTTGCTTTTAGAGATATGGTGATTGCAGTGAGGACCAGGAACACACAGACTGTGAGTGATTATAGCTGCAATGGCCGCCATGTGTTTACACAGACGAGGGAGCTTGAGAGACCACTTGTTGGTTCGATTTTACAGAGTATGTGGGGAGTGTCGCCGACTCATATGTTGTGGAGCCCTAGGCACAATACTACGTTGGTGGATTACACGTGGAGTGTTGGGCAGACTCCATTTGGGCCGTTCTCGGAGATTTCATCACTATCATTCGTACAGAAGGATGCTGCTCGGAGAAATGTTCTTTTGACATCACTAAATTACAGCATATCAAGTGCTGTAGATGTTCTTGAATCCATAGCTGCACATGGTGGGGAGGGAAAGCTCCTGAAACGTAATCGACATGTTGAGTTCGTACAAAGGTGGAATTTCTTCAAGTACAAGCTGGACAAAGCAGTTTCTGCAATGTCACATTTGGACTTTGAGATGGCTTTATACTATATGAGGTCCTCAGATCATGATCTATATGCTATCCACTCTATTGTGTATCATGCCTCCCAGGAATTGGAGGCATCCCTTGTATGTTTCAAGGATCCACCATTTCCGTGGTCTTTTGTGTTTATGGTTGCAGTGCTTCTCCTTCTTGGCTATTATATTAATTCTAGAAAACACAAACTATTCAGAAGTAAGATTACTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

693

Amino Acids

78.07

Weight (kDa)

6.86

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 143 - 312 1e-68 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 314, 1119
AccB1I GGYRCC 1 cut(s) 770
AccB7I CCANNNNNTGG 2 cut(s) 1508, 1945
AccBSI CCGCTC 2 cut(s) 553, 1008
AccI GTMKAC 2 cut(s) 783, 1181
AciI CCGC 9 cut(s) 171, 534, 551, 748, 845, 887, 1008, 1292, 1465
AclWI GGATC 8 cut(s) 35, 176, 593, 694, 767, 1101, 1967, 1980
AcoI YGGCCR 1 cut(s) 1462
AcsI RAATTY 6 cut(s) 113, 401, 430, 945, 964, 1807
AcvI CACGTG 1 cut(s) 1596
AcyI GRCGYC 1 cut(s) 771
AdeI CACNNNGTG 2 cut(s) 717, 1751
AfaI GTAC 5 cut(s) 54, 215, 1656, 1797, 1820
AfiI CCNNNNNNNGG 4 cut(s) 623, 906, 1508, 1945
AflIII ACRYGT 3 cut(s) 988, 1593, 1783
AgeI ACCGGT 1 cut(s) 778
AgsI TTSAA 7 cut(s) 245, 254, 956, 1111, 1733, 1816, 1969
AjnI CCWGG 5 cut(s) 703, 918, 1163, 1424, 1938
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 4 cut(s) 417, 545, 562, 1492
AlwI GGATC 8 cut(s) 35, 176, 593, 694, 767, 1101, 1967, 1980
AlwNI CAGNNNCTG 1 cut(s) 1841
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 5 cut(s) 653, 790, 1078, 1462, 1622
ApaI GGGCCC 1 cut(s) 657
ApeKI GCWGC 4 cut(s) 1366, 1455, 1667, 1742
ApoI RAATTY 6 cut(s) 113, 401, 430, 945, 964, 1807
AseI ATTAAT 2 cut(s) 1173, 2037
AsiGI ACCGGT 1 cut(s) 778
Asp700I GAANNNNTTC 2 cut(s) 258, 1811
AspA2I CCTAGG 1 cut(s) 1565
AspLEI GCGC 3 cut(s) 7, 773, 799
AsuC2I CCSGG 2 cut(s) 739, 794
AsuHPI GGTGA 6 cut(s) 485, 703, 1013, 1135, 1373, 1420
AsuII TTCGAA 1 cut(s) 1238
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 7 cut(s) 619, 735, 745, 776, 1050, 1422, 1887
AvrII CCTAGG 1 cut(s) 1565
AxyI CCTNAGG 1 cut(s) 1302
BaeGI GKGCMC 2 cut(s) 657, 1142
BamHI GGATCC 1 cut(s) 1972
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 3 cut(s) 522, 657, 1565
BarI GAAGNNNNNNTAC 1 cut(s) 2056
BbrPI CACGTG 1 cut(s) 1596
Bbv12I GWGCWC 1 cut(s) 522
BbvCI CCTCAGC 1 cut(s) 1272
BbvI GCAGC 4 cut(s) 1353, 1442, 1654, 1729
BccI CCATC 4 cut(s) 20, 76, 337, 1861
BceAI ACGGC 4 cut(s) 640, 805, 871, 1609
BcgI CGANNNNNNTGC 2 cut(s) 1228, 1262
BciT130I CCWGG 5 cut(s) 705, 920, 1165, 1426, 1940
BcnI CCSGG 2 cut(s) 739, 794
BcoDI GTCTC 4 cut(s) 417, 545, 562, 1492
BfaI CTAG 4 cut(s) 234, 509, 1566, 2043
BfmI CTRYAG 1 cut(s) 1719
BfoI RGCGCY 3 cut(s) 8, 774, 800
BisI GCNGC 7 cut(s) 551, 846, 1367, 1456, 1465, 1668, 1743
BlnI CCTAGG 1 cut(s) 1565
BlsI GCNGC 7 cut(s) 552, 847, 1368, 1457, 1466, 1669, 1744
Bme1390I CCNGG 7 cut(s) 705, 739, 794, 920, 1165, 1426, 1940
Bme18I GGWCC 7 cut(s) 619, 735, 745, 776, 1050, 1422, 1887
BmeT110I CYCGRG 1 cut(s) 144
BmiI GGNNCC 6 cut(s) 654, 655, 656, 772, 1562, 1974
BmrFI CCNGG 7 cut(s) 705, 739, 794, 920, 1165, 1426, 1940
BmsI GCATC 3 cut(s) 88, 1654, 1961
BoxI GACNNNNGTC 1 cut(s) 482
Bpu10I CCTNAGC 2 cut(s) 1068, 1272
Bpu14I TTCGAA 1 cut(s) 1238
BpuEI CTTGAG 1 cut(s) 1514
BpuMI CCSGG 2 cut(s) 739, 794
Bsa29I ATCGAT 2 cut(s) 762, 1040
BsaAI YACGTR 1 cut(s) 1596
BsaBI GATNNNNATC 4 cut(s) 45, 133, 1377, 1899
BsaHI GRCGYC 1 cut(s) 771
BsaI GGTCTC 1 cut(s) 1492
BsaJI CCNNGG 6 cut(s) 442, 622, 900, 1565, 1938, 1985
BsaWI WCCGGW 2 cut(s) 582, 778
Bsc4I CCNNNNNNNGG 4 cut(s) 623, 906, 1508, 1945
Bse118I RCCGGY 2 cut(s) 616, 778
Bse1I ACTGG 1 cut(s) 537
Bse21I CCTNAGG 1 cut(s) 1302
Bse3DI GCAATG 3 cut(s) 25, 1465, 1851
Bse8I GATNNNNATC 4 cut(s) 45, 133, 1377, 1899
BseBI CCWGG 5 cut(s) 705, 920, 1165, 1426, 1940
BseCI ATCGAT 2 cut(s) 762, 1040
BseDI CCNNGG 6 cut(s) 442, 622, 900, 1565, 1938, 1985
BseGI GGATG 4 cut(s) 835, 1066, 1669, 1952
BseJI GATNNNNATC 4 cut(s) 45, 133, 1377, 1899
BseLI CCNNNNNNNGG 4 cut(s) 623, 906, 1508, 1945
BseMI GCAATG 3 cut(s) 25, 1465, 1851
BseMII CTCAG 7 cut(s) 129, 254, 884, 932, 1263, 1293, 1905
BseNI ACTGG 1 cut(s) 537
BseRI GAGGAG 4 cut(s) 97, 688, 1289, 1319
BseSI GKGCMC 2 cut(s) 657, 1142
BseXI GCAGC 4 cut(s) 1353, 1442, 1654, 1729
BsgI GTGCAG 1 cut(s) 1728
Bsh1285I CGRYCG 3 cut(s) 548, 583, 783
BshFI GGCC 5 cut(s) 655, 792, 1080, 1464, 1624
BshNI GGYRCC 1 cut(s) 770
BshTI ACCGGT 1 cut(s) 778
BshVI ATCGAT 2 cut(s) 762, 1040
BsiEI CGRYCG 3 cut(s) 548, 583, 783
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 7 cut(s) 583, 617, 738, 774, 779, 794, 804
BslI CCNNNNNNNGG 4 cut(s) 623, 906, 1508, 1945
BsmAI GTCTC 4 cut(s) 417, 545, 562, 1492
BsmBI CGTCTC 1 cut(s) 562
BsnI GGCC 5 cut(s) 655, 792, 1080, 1464, 1624
Bso31I GGTCTC 1 cut(s) 1492
BsoBI CYCGRG 1 cut(s) 144
Bsp119I TTCGAA 1 cut(s) 1238
Bsp120I GGGCCC 1 cut(s) 653
Bsp1286I GDGCHC 4 cut(s) 522, 657, 1142, 1565
BspACI CCGC 9 cut(s) 171, 534, 551, 748, 845, 887, 1008, 1292, 1465
BspANI GGCC 5 cut(s) 655, 792, 1080, 1464, 1624
BspCNI CTCAG 7 cut(s) 130, 255, 883, 933, 1264, 1294, 1904
BspDI ATCGAT 2 cut(s) 762, 1040
BspHI TCATGA 1 cut(s) 1897
BspLI GGNNCC 6 cut(s) 654, 655, 656, 772, 1562, 1974
BspPI GGATC 8 cut(s) 35, 176, 593, 694, 767, 1101, 1967, 1980
BspT104I TTCGAA 1 cut(s) 1238
BspT107I GGYRCC 1 cut(s) 770
BspTNI GGTCTC 1 cut(s) 1492
BsrBI CCGCTC 2 cut(s) 553, 1008
BsrDI GCAATG 3 cut(s) 25, 1465, 1851
BsrFI RCCGGY 2 cut(s) 616, 778
BsrI ACTGG 1 cut(s) 537
BssAI RCCGGY 2 cut(s) 616, 778
BssECI CCNNGG 6 cut(s) 442, 622, 900, 1565, 1938, 1985
BssNAI GTATAC 1 cut(s) 1182
BssNI GRCGYC 1 cut(s) 771
BssT1I CCWWGG 2 cut(s) 900, 1565
Bst1107I GTATAC 1 cut(s) 1182
Bst2UI CCWGG 5 cut(s) 705, 920, 1165, 1426, 1940
Bst4CI ACNGT 6 cut(s) 193, 491, 812, 1390, 1441, 2077
Bst6I CTCTTC 2 cut(s) 249, 1235
BstACI GRCGYC 1 cut(s) 771
BstAPI GCANNNNNTGC 1 cut(s) 1841
BstBAI YACGTR 1 cut(s) 1596
BstBI TTCGAA 1 cut(s) 1238
BstC8I GCNNGC 4 cut(s) 157, 522, 860, 885
BstDEI CTNAG 8 cut(s) 138, 263, 870, 941, 1068, 1272, 1302, 1891
BstDSI CCRYGG 2 cut(s) 622, 1985
BstF5I GGATG 4 cut(s) 835, 1066, 1669, 1952
BstH2I RGCGCY 3 cut(s) 8, 774, 800
BstHHI GCGC 3 cut(s) 7, 773, 799
BstMAI GTCTC 4 cut(s) 417, 545, 562, 1492
BstMCI CGRYCG 3 cut(s) 548, 583, 783
BstMWI GCNNNNNNNGC 9 cut(s) 273, 859, 875, 884, 893, 1366, 1461, 1464, 1841
BstNI CCWGG 5 cut(s) 705, 920, 1165, 1426, 1940
BstNSI RCATGY 3 cut(s) 992, 1360, 1787
BstPAI GACNNNNGTC 1 cut(s) 482
BstSCI CCNGG 7 cut(s) 703, 737, 792, 918, 1163, 1424, 1938
BstSFI CTRYAG 1 cut(s) 1719
BstSLI GKGCMC 2 cut(s) 657, 1142
BstV1I GCAGC 4 cut(s) 1353, 1442, 1654, 1729
BstX2I RGATCY 3 cut(s) 40, 686, 1972
BstXI CCANNNNNNTGG 2 cut(s) 721, 1986
BstYI RGATCY 3 cut(s) 40, 686, 1972
BstZ17I GTATAC 1 cut(s) 1182
Bsu15I ATCGAT 2 cut(s) 762, 1040
Bsu36I CCTNAGG 1 cut(s) 1302
BsuRI GGCC 5 cut(s) 655, 792, 1080, 1464, 1624
BsuTUI ATCGAT 2 cut(s) 762, 1040
BtgI CCRYGG 2 cut(s) 622, 1985
BtsCI GGATG 4 cut(s) 835, 1066, 1669, 1952
BtsI GCAGTG 2 cut(s) 1422, 2016
BtsIMutI CAGTG 3 cut(s) 189, 1422, 2016
Cac8I GCNNGC 4 cut(s) 157, 522, 860, 885
CaiI CAGNNNCTG 1 cut(s) 1841
CciI TCATGA 1 cut(s) 1897
CfoI GCGC 3 cut(s) 7, 773, 799
Cfr10I RCCGGY 2 cut(s) 616, 778
ClaI ATCGAT 2 cut(s) 762, 1040
CpoI CGGWCCG 2 cut(s) 745, 776
CsiI ACCWGGT 1 cut(s) 918
Csp6I GTAC 5 cut(s) 53, 214, 1655, 1796, 1819
CspAI ACCGGT 1 cut(s) 778
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CspI CGGWCCG 2 cut(s) 745, 776
CviAII CATG 8 cut(s) 67, 989, 1357, 1469, 1748, 1784, 1898, 1931
CviQI GTAC 5 cut(s) 53, 214, 1655, 1796, 1819
DdeI CTNAG 8 cut(s) 138, 263, 870, 941, 1068, 1272, 1302, 1891
DinI GGCGCC 1 cut(s) 772
DraI TTTAAA 1 cut(s) 420
DraIII CACNNNGTG 2 cut(s) 717, 1751
EaeI YGGCCR 1 cut(s) 1462
Eam1104I CTCTTC 2 cut(s) 249, 1235
EarI CTCTTC 2 cut(s) 249, 1235
EciI GGCGGA 1 cut(s) 902
Ecl136II GAGCTC 1 cut(s) 520
Eco130I CCWWGG 2 cut(s) 900, 1565
Eco24I GRGCYC 3 cut(s) 522, 657, 1565
Eco31I GGTCTC 1 cut(s) 1492
Eco47I GGWCC 7 cut(s) 619, 735, 745, 776, 1050, 1422, 1887
Eco53kI GAGCTC 1 cut(s) 520
Eco72I CACGTG 1 cut(s) 1596
Eco81I CCTNAGG 1 cut(s) 1302
Eco88I CYCGRG 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 520
EcoO109I RGGNCCY 4 cut(s) 653, 654, 1050, 1887
EcoRI GAATTC 3 cut(s) 113, 945, 964
EcoRII CCWGG 5 cut(s) 703, 918, 1163, 1424, 1938
EcoT14I CCWWGG 2 cut(s) 900, 1565
EcoT38I GRGCYC 3 cut(s) 522, 657, 1565
EgeI GGCGCC 1 cut(s) 772
EheI GGCGCC 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 900, 1565
Esp3I CGTCTC 1 cut(s) 562
FaeI CATG 8 cut(s) 70, 992, 1360, 1472, 1751, 1787, 1901, 1934
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FatI CATG 8 cut(s) 66, 988, 1356, 1468, 1747, 1783, 1897, 1930
FauI CCCGC 2 cut(s) 541, 1285
FauNDI CATATG 1 cut(s) 1551
FblI GTMKAC 2 cut(s) 783, 1181
Fnu4HI GCNGC 7 cut(s) 551, 846, 1367, 1456, 1465, 1668, 1743
FokI GGATG 4 cut(s) 822, 1073, 1676, 1939
FriOI GRGCYC 3 cut(s) 522, 657, 1565
Fsp4HI GCNGC 7 cut(s) 551, 846, 1367, 1456, 1465, 1668, 1743
FspBI CTAG 4 cut(s) 234, 509, 1566, 2043
GlaI GCGC 3 cut(s) 6, 772, 798
GluI GCNGC 7 cut(s) 551, 846, 1367, 1456, 1465, 1668, 1743
HaeII RGCGCY 3 cut(s) 8, 774, 800
HaeIII GGCC 5 cut(s) 655, 792, 1080, 1464, 1624
HapII CCGG 7 cut(s) 583, 617, 738, 774, 779, 794, 804
HhaI GCGC 3 cut(s) 7, 773, 799
Hin1I GRCGYC 1 cut(s) 771
Hin1II CATG 8 cut(s) 70, 992, 1360, 1472, 1751, 1787, 1901, 1934
Hin6I GCGC 3 cut(s) 5, 771, 797
HinP1I GCGC 3 cut(s) 5, 771, 797
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HpaII CCGG 7 cut(s) 583, 617, 738, 774, 779, 794, 804
HphI GGTGA 6 cut(s) 485, 703, 1013, 1135, 1373, 1420
Hpy166II GTNNAC 8 cut(s) 53, 216, 487, 622, 631, 784, 1182, 1476
Hpy8I GTNNAC 8 cut(s) 53, 216, 487, 622, 631, 784, 1182, 1476
Hpy99I CGWCG 3 cut(s) 566, 812, 1189
HpyAV CCTTC 3 cut(s) 868, 1654, 2030
HpyCH4III ACNGT 6 cut(s) 193, 491, 812, 1390, 1441, 2077
HpyCH4IV ACGT 5 cut(s) 1184, 1211, 1580, 1595, 1774
HpyCH4V TGCA 8 cut(s) 159, 853, 1249, 1415, 1458, 1745, 1844, 2009
HpyF10VI GCNNNNNNNGC 9 cut(s) 273, 859, 875, 884, 893, 1366, 1461, 1464, 1841
HpyF3I CTNAG 8 cut(s) 138, 263, 870, 941, 1068, 1272, 1302, 1891
HpySE526I ACGT 5 cut(s) 1184, 1211, 1580, 1595, 1774
Hsp92I GRCGYC 1 cut(s) 771
Hsp92II CATG 8 cut(s) 70, 992, 1360, 1472, 1751, 1787, 1901, 1934
HspAI GCGC 3 cut(s) 5, 771, 797
KasI GGCGCC 1 cut(s) 770
LmnI GCTCC 4 cut(s) 726, 1489, 1560, 1770
Lsp1109I GCAGC 4 cut(s) 1353, 1442, 1654, 1729
LweI GCATC 3 cut(s) 88, 1654, 1961
MabI ACCWGGT 1 cut(s) 918
MaeI CTAG 4 cut(s) 234, 509, 1566, 2043
MaeII ACGT 5 cut(s) 1184, 1211, 1580, 1595, 1774
MaeIII GTNAC 2 cut(s) 491, 1848
MbiI CCGCTC 2 cut(s) 553, 1008
MboII GAAGA 4 cut(s) 266, 917, 1252, 1804
MfeI CAATTG 1 cut(s) 249
MflI RGATCY 3 cut(s) 40, 686, 1972
MhlI GDGCHC 4 cut(s) 522, 657, 1142, 1565
Mly113I GGCGCC 1 cut(s) 771
MlyI GAGTC 6 cut(s) 492, 566, 689, 1259, 1540, 1606
MmeI TCCRAC 2 cut(s) 375, 723
MroXI GAANNNNTTC 2 cut(s) 258, 1811
MseI TTAA 6 cut(s) 366, 405, 419, 434, 1173, 2037
MslI CAYNNNNRTG 2 cut(s) 993, 1984
MspI CCGG 7 cut(s) 583, 617, 738, 774, 779, 794, 804
MspR9I CCNGG 7 cut(s) 705, 739, 794, 920, 1165, 1426, 1940
MunI CAATTG 1 cut(s) 249
MvaI CCWGG 5 cut(s) 705, 920, 1165, 1426, 1940
MwoI GCNNNNNNNGC 9 cut(s) 273, 859, 875, 884, 893, 1366, 1461, 1464, 1841
NarI GGCGCC 1 cut(s) 771
NciI CCSGG 2 cut(s) 739, 794
NdeI CATATG 1 cut(s) 1551
NlaIII CATG 8 cut(s) 70, 992, 1360, 1472, 1751, 1787, 1901, 1934
NlaIV GGNNCC 6 cut(s) 654, 655, 656, 772, 1562, 1974
NmuCI GTSAC 2 cut(s) 491, 1848
NspI RCATGY 3 cut(s) 992, 1360, 1787
NspV TTCGAA 1 cut(s) 1238
PaeR7I CTCGAG 1 cut(s) 144
PagI TCATGA 1 cut(s) 1897
PciI ACATGT 2 cut(s) 988, 1783
PcsI WCGNNNNNNNCGW 5 cut(s) 576, 1139, 1175, 1184, 1217
PdmI GAANNNNTTC 2 cut(s) 258, 1811
PfeI GAWTC 5 cut(s) 202, 259, 281, 1235, 1733
PflFI GACNNNGTC 2 cut(s) 739, 812
PflMI CCANNNNNTGG 2 cut(s) 1508, 1945
PinAI ACCGGT 1 cut(s) 778
PkrI GCNGC 7 cut(s) 552, 847, 1368, 1457, 1466, 1669, 1744
Ple19I CGATCG 1 cut(s) 548
PleI GAGTC 6 cut(s) 491, 566, 689, 1259, 1540, 1606
PluTI GGCGCC 1 cut(s) 774
PmaCI CACGTG 1 cut(s) 1596
PmlI CACGTG 1 cut(s) 1596
PpsI GAGTC 6 cut(s) 491, 566, 689, 1259, 1540, 1606
Ppu21I YACGTR 1 cut(s) 1596
PpuMI RGGWCCY 2 cut(s) 1050, 1887
PscI ACATGT 2 cut(s) 988, 1783
PshAI GACNNNNGTC 1 cut(s) 482
PshBI ATTAAT 2 cut(s) 1173, 2037
PsiI TTATAA 2 cut(s) 314, 1119
Psp124BI GAGCTC 1 cut(s) 522
Psp5II RGGWCCY 2 cut(s) 1050, 1887
Psp6I CCWGG 5 cut(s) 703, 918, 1163, 1424, 1938
PspCI CACGTG 1 cut(s) 1596
PspGI CCWGG 5 cut(s) 703, 918, 1163, 1424, 1938
PspN4I GGNNCC 6 cut(s) 654, 655, 656, 772, 1562, 1974
PspOMI GGGCCC 1 cut(s) 653
PspPPI RGGWCCY 2 cut(s) 1050, 1887
PspXI VCTCGAGB 1 cut(s) 144
PstNI CAGNNNCTG 1 cut(s) 1841
PsuI RGATCY 3 cut(s) 40, 686, 1972
PsyI GACNNNGTC 2 cut(s) 739, 812
PvuI CGATCG 1 cut(s) 548
RsaI GTAC 5 cut(s) 54, 215, 1656, 1797, 1820
RsaNI GTAC 5 cut(s) 53, 214, 1655, 1796, 1819
RseI CAYNNNNRTG 2 cut(s) 993, 1984
Rsr2I CGGWCCG 2 cut(s) 745, 776
RsrII CGGWCCG 2 cut(s) 745, 776
SacI GAGCTC 1 cut(s) 522
SalI GTCGAC 1 cut(s) 782
SaqAI TTAA 6 cut(s) 366, 405, 419, 434, 1173, 2037
SatI GCNGC 7 cut(s) 551, 846, 1367, 1456, 1465, 1668, 1743
SchI GAGTC 6 cut(s) 492, 566, 689, 1259, 1540, 1606
ScrFI CCNGG 7 cut(s) 705, 739, 794, 920, 1165, 1426, 1940
SduI GDGCHC 4 cut(s) 522, 657, 1142, 1565
SexAI ACCWGGT 1 cut(s) 918
SfaNI GCATC 3 cut(s) 88, 1654, 1961
SfcI CTRYAG 1 cut(s) 1719
SfoI GGCGCC 1 cut(s) 772
Sfr274I CTCGAG 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 1238
SinI GGWCC 7 cut(s) 619, 735, 745, 776, 1050, 1422, 1887
SlaI CTCGAG 1 cut(s) 144
SmiMI CAYNNNNRTG 2 cut(s) 993, 1984
SmlI CTYRAG 2 cut(s) 144, 1493
SmoI CTYRAG 2 cut(s) 144, 1493
SsiI CCGC 9 cut(s) 171, 534, 551, 748, 845, 887, 1008, 1292, 1465
SspDI GGCGCC 1 cut(s) 770
SspMI CTAG 4 cut(s) 234, 509, 1566, 2043
SstI GAGCTC 1 cut(s) 522
StyD4I CCNGG 7 cut(s) 703, 737, 792, 918, 1163, 1424, 1938
StyI CCWWGG 2 cut(s) 900, 1565
TaaI ACNGT 6 cut(s) 193, 491, 812, 1390, 1441, 2077
TaiI ACGT 5 cut(s) 1187, 1214, 1583, 1598, 1777
TatI WGTACW 2 cut(s) 213, 1818
TauI GCSGC 3 cut(s) 553, 848, 1467
TfiI GAWTC 5 cut(s) 202, 259, 281, 1235, 1733
Tru1I TTAA 6 cut(s) 366, 405, 419, 434, 1173, 2037
Tru9I TTAA 6 cut(s) 366, 405, 419, 434, 1173, 2037
TscAI CASTG 3 cut(s) 196, 1422, 2016
TseFI GTSAC 2 cut(s) 491, 1848
TseI GCWGC 4 cut(s) 1366, 1455, 1667, 1742
Tsp45I GTSAC 2 cut(s) 491, 1848
TspDTI ATGAA 3 cut(s) 824, 1140, 1629
TspGWI ACGGA 3 cut(s) 70, 550, 1974
TspRI CASTG 3 cut(s) 196, 1422, 2016
Tth111I GACNNNGTC 2 cut(s) 739, 812
Van91I CCANNNNNTGG 2 cut(s) 1508, 1945
VpaK11BI GGWCC 7 cut(s) 619, 735, 745, 776, 1050, 1422, 1887
VspI ATTAAT 2 cut(s) 1173, 2037
XapI RAATTY 6 cut(s) 113, 401, 430, 945, 964, 1807
XbaI TCTAGA 1 cut(s) 2042
XceI RCATGY 3 cut(s) 992, 1360, 1787
XcmI CCANNNNNNNNNTGG 1 cut(s) 1983
XhoI CTCGAG 1 cut(s) 144
XmaJI CCTAGG 1 cut(s) 1565
XmiI GTMKAC 2 cut(s) 783, 1181
XmnI GAANNNNTTC 2 cut(s) 258, 1811
XspI CTAG 4 cut(s) 234, 509, 1566, 2043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.