Rmu_ssc0000393.1_g000028

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000393.1
OG0012436
Physical Location & Seq
Forward (+)
167373 .. 171455
4083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000393.1_g000028.1.cds

Sequence Viewer

Length: 2493 bp
atgccgactcctcctacactcagaacaccttacactctgtctttgtctacattcattgtgaatgtgggtgattcaaagtgtctttcagacctctcaagtgacgatgatggtgattcaaagggtcttcagatcctttcaactggcagcggcaacgaaatatggagctcaagtgtcactagccatgtggaagagaaaccctcctctaggtatcctgaaatgaactctacacaacataaagcacactgtttgcacccaaggttccttcaattcttaaagatagatcctctactccatggtcagaatgatattgatgtgcttgctggagagactgacaatgcaaagggacagtgcaatgcaggggatgagaatacaagcgaatcatatttcattcatgactatgattctcgcaagaagcacattccactagatttgttgaaacaactgaagagattcctgagcatatacagcttccaccctccactttgcaccgccctcctactcctcctaaccctaatcgccacctccacctccacctcagccccaattctcggcctcgacaccttcctcacccaccagtcccgctacgaccggcaagcctccaacgactccttccagtctctctcctccactctccgccactccctttctctctcctcgccgtctctctcccactctctctcctccatcctctccctcaccattcctctctctctccacgtccgcctcgtcggcccttccttcccttcctcctccgccactctcctctcctccttcctctccgcctcccagacctccgatcacttccacgtcatcacccccgccgacgccgccgactctcaccgcttggccgtcaagcactccctccacctcgacgtcagtcactccccctccctcgcctctcagctctccaaaaccctagcctccgagctcgccaatacccccgccagtctccgatcgccgctcgtctccgtcgcctacgactcaatcgaccggatcatcaagcaggacttcgacaaagagaagccggtccacggcgtttacctctatttcttggatttggggccccaatccaagagctatgcttacagctatggatctggtgactcctctcctggcttcaccaagtgcttgggcagcgtttggaccgggtcggaccgctacatttggatcgatttgggcgccggaccggtcgactacggcccggcgctttccggcgacggtgtcttgccgaaaggcgagtttcatcctctggcggcgttgcacggctcgccgaaagctcagaaggctttgctggcggacttggcttccttggtttggagtgcttaccaggttcttcttgtcccctctttgagaattcctgttcagtatgagaattctttgattgttcagtttatacatgtttatggaggtgagagtggtaaggattcgagtggtttggattggaaatcgattgagaggacctttatggatgaggctaaggataatggcttgttgttaggggatcagtctttgagtttcaagaattataaggtgaagtattcggagtgcccgatatgttcatttgctattgccaggtcgattaattcatatacgtcgaggttcttgtttgataactatacgttgattgcaagtgagtatttggattcgaagagattgcatcagatattgtcagactctgctgatgagtttaggagagtggcagggtttcctgaggaggattttggcagggtgcttcctgtgtatgtgtttgatttggactaccacatgctgttgctgctcgataggtatcaccaaactgttgcttttagagatatggtgattgcagtgaggaccaggaacacacagactgtgagtgattatagctgcaatggccgccatgtgtttacacagacgagggagcttgagagaccacttgttggttcgattttacagagtatgtggggagtgtcgccgactcatatgttgtggagccctaggcacaatactacgttggtggattacacgtggagtgttgggcagactccatttgggccgttctcggagatttcatcactatcattcgtacagaaggatgctgctcggagaaatgttcttttgacatcactaaattacagcatatcaagtgctgtagatgttctcgaatccatagctgcacatggtggggagagaaagctcctgaaacgtaatcgacatgttgagttcgtacaaaggtggaatttcttcaagtacaagctggacaaagcagtttctgcaatgtcacatttagactttgagatggctttatactatatgaggtcctcagatcatgatctatatgctatccactctattgtgtatcatgcctcccaggaattggaggcatcccttgtatgtttcaaggatccaccatttccgtggtcttttgtgtttatggttgcagtgcttctccttcttggctattatattagttctagaaaacacaaactattcagaagtaagagtaagcaattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

830

Amino Acids

92.62

Weight (kDa)

6.58

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1527
AatII GACGTC 1 cut(s) 876
AccB1I GGYRCC 1 cut(s) 1178
AccB7I CCANNNNNTGG 2 cut(s) 1916, 2353
AccBSI CCGCTC 1 cut(s) 961
AccI GTMKAC 2 cut(s) 47, 1191
AclWI GGATC 8 cut(s) 124, 275, 1001, 1102, 1175, 1509, 2375, 2388
AcoI YGGCCR 2 cut(s) 846, 1870
AcsI RAATTY 3 cut(s) 1353, 1372, 2215
AcuI CTGAAG 2 cut(s) 110, 464
AcvI CACGTG 1 cut(s) 2004
AcyI GRCGYC 3 cut(s) 825, 873, 1179
AdeI CACNNNGTG 2 cut(s) 1125, 2159
AfaI GTAC 3 cut(s) 2064, 2205, 2228
AfiI CCNNNNNNNGG 6 cut(s) 204, 548, 1031, 1314, 1916, 2353
AflIII ACRYGT 3 cut(s) 1396, 2001, 2191
AgeI ACCGGT 1 cut(s) 1186
AgsI TTSAA 8 cut(s) 75, 117, 138, 266, 436, 1519, 2224, 2377
AjiI CACGTC 2 cut(s) 718, 808
AjnI CCWGG 5 cut(s) 1111, 1326, 1571, 1832, 2346
AjuI GAANNNNNNNTTGG 2 cut(s) 593, 625
Alw21I GWGCWC 2 cut(s) 167, 930
Alw26I GTCTC 6 cut(s) 320, 621, 666, 953, 970, 1900
AlwI GGATC 8 cut(s) 124, 275, 1001, 1102, 1175, 1509, 2375, 2388
AlwNI CAGNNNCTG 2 cut(s) 1676, 2249
AoxI GGCC 7 cut(s) 550, 730, 846, 1061, 1198, 1870, 2030
ApaI GGGCCC 1 cut(s) 1065
ApeKI GCWGC 6 cut(s) 144, 1134, 1774, 1863, 2075, 2150
ApoI RAATTY 3 cut(s) 1353, 1372, 2215
AseI ATTAAT 1 cut(s) 1581
AsiGI ACCGGT 1 cut(s) 1186
Asp700I GAANNNNTTC 2 cut(s) 449, 2219
AspA2I CCTAGG 1 cut(s) 1973
AspLEI GCGC 2 cut(s) 1181, 1207
AsuC2I CCSGG 2 cut(s) 1147, 1202
AsuII TTCGAA 1 cut(s) 1646
AvaII GGWCC 7 cut(s) 1027, 1143, 1153, 1184, 1458, 1830, 2295
AvrII CCTAGG 1 cut(s) 1973
AxyI CCTNAGG 1 cut(s) 1710
BaeGI GKGCMC 2 cut(s) 1065, 1550
BamHI GGATCC 1 cut(s) 2380
BanI GGYRCC 1 cut(s) 1178
BanII GRGCYC 4 cut(s) 167, 930, 1065, 1973
BbrPI CACGTG 1 cut(s) 2004
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 2 cut(s) 167, 930
BbvCI CCTCAGC 1 cut(s) 535
BbvI GCAGC 6 cut(s) 156, 1146, 1761, 1850, 2062, 2137
BccI CCATC 3 cut(s) 101, 692, 2269
BceAI ACGGC 6 cut(s) 643, 833, 1048, 1213, 1279, 2017
BcgI CGANNNNNNTGC 2 cut(s) 1636, 1670
BciT130I CCWGG 5 cut(s) 1113, 1328, 1573, 1834, 2348
BciVI GTATCC 1 cut(s) 219
BcnI CCSGG 2 cut(s) 1147, 1202
BcoDI GTCTC 6 cut(s) 320, 621, 666, 953, 970, 1900
BfaI CTAG 6 cut(s) 177, 204, 425, 917, 1974, 2451
BfmI CTRYAG 1 cut(s) 2127
BfoI RGCGCY 2 cut(s) 1182, 1208
BfuI GTATCC 1 cut(s) 219
BglI GCCNNNNNGGC 1 cut(s) 729
BlnI CCTAGG 1 cut(s) 1973
Bme1390I CCNGG 7 cut(s) 1113, 1147, 1202, 1328, 1573, 1834, 2348
Bme18I GGWCC 7 cut(s) 1027, 1143, 1153, 1184, 1458, 1830, 2295
BmgBI CACGTC 2 cut(s) 718, 808
BmiI GGNNCC 7 cut(s) 260, 1062, 1063, 1064, 1180, 1970, 2382
BmrFI CCNGG 7 cut(s) 1113, 1147, 1202, 1328, 1573, 1834, 2348
BmsI GCATC 3 cut(s) 1666, 2062, 2369
BoxI GACNNNNGTC 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 3 cut(s) 455, 535, 1476
Bpu14I TTCGAA 1 cut(s) 1646
BpuEI CTTGAG 3 cut(s) 79, 151, 1922
BpuMI CCSGG 2 cut(s) 1147, 1202
Bsa29I ATCGAT 2 cut(s) 1170, 1448
BsaAI YACGTR 1 cut(s) 2004
BsaBI GATNNNNATC 2 cut(s) 1785, 2307
BsaHI GRCGYC 3 cut(s) 825, 873, 1179
BsaI GGTCTC 1 cut(s) 1900
BsaJI CCNNGG 7 cut(s) 254, 292, 1030, 1308, 1973, 2346, 2393
BsaWI WCCGGW 2 cut(s) 990, 1186
BsaXI ACNNNNNCTCC 6 cut(s) 154, 184, 662, 692, 872, 902
Bsc4I CCNNNNNNNGG 6 cut(s) 204, 548, 1031, 1314, 1916, 2353
Bse118I RCCGGY 3 cut(s) 588, 1024, 1186
Bse1I ACTGG 4 cut(s) 145, 574, 613, 945
Bse21I CCTNAGG 1 cut(s) 1710
Bse3DI GCAATG 3 cut(s) 358, 1873, 2259
Bse8I GATNNNNATC 2 cut(s) 1785, 2307
BseBI CCWGG 5 cut(s) 1113, 1328, 1573, 1834, 2348
BseCI ATCGAT 2 cut(s) 1170, 1448
BseDI CCNNGG 7 cut(s) 254, 292, 1030, 1308, 1973, 2346, 2393
BseGI GGATG 6 cut(s) 367, 684, 1243, 1474, 2077, 2360
BseJI GATNNNNATC 2 cut(s) 1785, 2307
BseLI CCNNNNNNNGG 6 cut(s) 204, 548, 1031, 1314, 1916, 2353
BseMI GCAATG 3 cut(s) 358, 1873, 2259
BseMII CTCAG 7 cut(s) 34, 446, 549, 914, 1292, 1701, 2313
BseNI ACTGG 4 cut(s) 145, 574, 613, 945
BseSI GKGCMC 2 cut(s) 1065, 1550
BseXI GCAGC 6 cut(s) 156, 1146, 1761, 1850, 2062, 2137
BsgI GTGCAG 1 cut(s) 2136
Bsh1285I CGRYCG 4 cut(s) 589, 956, 991, 1191
BshFI GGCC 7 cut(s) 552, 732, 848, 1063, 1200, 1872, 2032
BshNI GGYRCC 1 cut(s) 1178
BshTI ACCGGT 1 cut(s) 1186
BshVI ATCGAT 2 cut(s) 1170, 1448
BsiEI CGRYCG 4 cut(s) 589, 956, 991, 1191
BsiHKAI GWGCWC 2 cut(s) 167, 930
BsiSI CCGG 8 cut(s) 589, 991, 1025, 1146, 1182, 1187, 1202, 1212
BslFI GGGAC 3 cut(s) 357, 562, 1325
BslI CCNNNNNNNGG 6 cut(s) 204, 548, 1031, 1314, 1916, 2353
BsmAI GTCTC 6 cut(s) 320, 621, 666, 953, 970, 1900
BsmBI CGTCTC 2 cut(s) 666, 970
BsmFI GGGAC 3 cut(s) 357, 562, 1325
BsnI GGCC 7 cut(s) 552, 732, 848, 1063, 1200, 1872, 2032
Bso31I GGTCTC 1 cut(s) 1900
Bsp119I TTCGAA 1 cut(s) 1646
Bsp120I GGGCCC 1 cut(s) 1061
Bsp1286I GDGCHC 5 cut(s) 167, 930, 1065, 1550, 1973
Bsp19I CCATGG 1 cut(s) 292
BspANI GGCC 7 cut(s) 552, 732, 848, 1063, 1200, 1872, 2032
BspCNI CTCAG 7 cut(s) 33, 447, 548, 913, 1291, 1702, 2312
BspDI ATCGAT 2 cut(s) 1170, 1448
BspHI TCATGA 2 cut(s) 391, 2305
BspLI GGNNCC 7 cut(s) 260, 1062, 1063, 1064, 1180, 1970, 2382
BspPI GGATC 8 cut(s) 124, 275, 1001, 1102, 1175, 1509, 2375, 2388
BspT104I TTCGAA 1 cut(s) 1646
BspT107I GGYRCC 1 cut(s) 1178
BspTNI GGTCTC 1 cut(s) 1900
BsrBI CCGCTC 1 cut(s) 961
BsrDI GCAATG 3 cut(s) 358, 1873, 2259
BsrFI RCCGGY 3 cut(s) 588, 1024, 1186
BsrI ACTGG 4 cut(s) 145, 574, 613, 945
BssAI RCCGGY 3 cut(s) 588, 1024, 1186
BssECI CCNNGG 7 cut(s) 254, 292, 1030, 1308, 1973, 2346, 2393
BssNI GRCGYC 3 cut(s) 825, 873, 1179
BssT1I CCWWGG 4 cut(s) 254, 292, 1308, 1973
Bst2UI CCWGG 5 cut(s) 1113, 1328, 1573, 1834, 2348
Bst4CI ACNGT 5 cut(s) 245, 348, 1220, 1798, 1849
Bst6I CTCTTC 3 cut(s) 183, 440, 1643
BstACI GRCGYC 3 cut(s) 825, 873, 1179
BstAPI GCANNNNNTGC 1 cut(s) 2249
BstBAI YACGTR 1 cut(s) 2004
BstBI TTCGAA 1 cut(s) 1646
BstC8I GCNNGC 5 cut(s) 318, 594, 930, 1268, 1293
BstDEI CTNAG 8 cut(s) 20, 455, 535, 900, 1278, 1476, 1710, 2299
BstDSI CCRYGG 3 cut(s) 292, 1030, 2393
BstENI CCTNNNNNAGG 1 cut(s) 202
BstF5I GGATG 6 cut(s) 367, 684, 1243, 1474, 2077, 2360
BstH2I RGCGCY 2 cut(s) 1182, 1208
BstHHI GCGC 2 cut(s) 1181, 1207
BstMAI GTCTC 6 cut(s) 320, 621, 666, 953, 970, 1900
BstMCI CGRYCG 4 cut(s) 589, 956, 991, 1191
BstNI CCWGG 5 cut(s) 1113, 1328, 1573, 1834, 2348
BstNSI RCATGY 3 cut(s) 1400, 1768, 2195
BstPAI GACNNNNGTC 1 cut(s) 876
BstSCI CCNGG 7 cut(s) 1111, 1145, 1200, 1326, 1571, 1832, 2346
BstSFI CTRYAG 1 cut(s) 2127
BstSLI GKGCMC 2 cut(s) 1065, 1550
BstV1I GCAGC 6 cut(s) 156, 1146, 1761, 1850, 2062, 2137
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 4 cut(s) 129, 280, 1094, 2380
BstXI CCANNNNNNTGG 2 cut(s) 1129, 2394
BstYI RGATCY 4 cut(s) 129, 280, 1094, 2380
Bsu15I ATCGAT 2 cut(s) 1170, 1448
Bsu36I CCTNAGG 1 cut(s) 1710
BsuI GTATCC 1 cut(s) 219
BsuRI GGCC 7 cut(s) 552, 732, 848, 1063, 1200, 1872, 2032
BsuTUI ATCGAT 2 cut(s) 1170, 1448
BtgI CCRYGG 3 cut(s) 292, 1030, 2393
BtrI CACGTC 2 cut(s) 718, 808
BtsCI GGATG 6 cut(s) 367, 684, 1243, 1474, 2077, 2360
BtsI GCAGTG 2 cut(s) 1830, 2424
BtsIMutI CAGTG 4 cut(s) 241, 353, 1830, 2424
Cac8I GCNNGC 5 cut(s) 318, 594, 930, 1268, 1293
CaiI CAGNNNCTG 2 cut(s) 1676, 2249
CciI TCATGA 2 cut(s) 391, 2305
CfoI GCGC 2 cut(s) 1181, 1207
Cfr10I RCCGGY 3 cut(s) 588, 1024, 1186
ClaI ATCGAT 2 cut(s) 1170, 1448
CpoI CGGWCCG 2 cut(s) 1153, 1184
CseI GACGC 1 cut(s) 833
CsiI ACCWGGT 1 cut(s) 1326
Csp6I GTAC 3 cut(s) 2063, 2204, 2227
CspAI ACCGGT 1 cut(s) 1186
CspCI CAANNNNNGTGG 2 cut(s) 411, 446
CspI CGGWCCG 2 cut(s) 1153, 1184
CviQI GTAC 3 cut(s) 2063, 2204, 2227
DdeI CTNAG 8 cut(s) 20, 455, 535, 900, 1278, 1476, 1710, 2299
DinI GGCGCC 1 cut(s) 1180
DraIII CACNNNGTG 2 cut(s) 1125, 2159
EaeI YGGCCR 2 cut(s) 846, 1870
Eam1104I CTCTTC 3 cut(s) 183, 440, 1643
EarI CTCTTC 3 cut(s) 183, 440, 1643
EciI GGCGGA 5 cut(s) 623, 710, 742, 769, 1310
Ecl136II GAGCTC 2 cut(s) 165, 928
Eco130I CCWWGG 4 cut(s) 254, 292, 1308, 1973
Eco24I GRGCYC 4 cut(s) 167, 930, 1065, 1973
Eco31I GGTCTC 1 cut(s) 1900
Eco47I GGWCC 7 cut(s) 1027, 1143, 1153, 1184, 1458, 1830, 2295
Eco53kI GAGCTC 2 cut(s) 165, 928
Eco57I CTGAAG 2 cut(s) 110, 464
Eco72I CACGTG 1 cut(s) 2004
Eco81I CCTNAGG 1 cut(s) 1710
EcoICRI GAGCTC 2 cut(s) 165, 928
EcoNI CCTNNNNNAGG 1 cut(s) 202
EcoO109I RGGNCCY 4 cut(s) 1061, 1062, 1458, 2295
EcoRI GAATTC 2 cut(s) 1353, 1372
EcoRII CCWGG 5 cut(s) 1111, 1326, 1571, 1832, 2346
EcoT14I CCWWGG 4 cut(s) 254, 292, 1308, 1973
EcoT38I GRGCYC 4 cut(s) 167, 930, 1065, 1973
EgeI GGCGCC 1 cut(s) 1180
EheI GGCGCC 1 cut(s) 1180
ErhI CCWWGG 4 cut(s) 254, 292, 1308, 1973
Esp3I CGTCTC 2 cut(s) 666, 970
FalI AAGNNNNNCTT 2 cut(s) 992, 1024
FaqI GGGAC 3 cut(s) 357, 562, 1325
FauI CCCGC 3 cut(s) 587, 826, 949
FauNDI CATATG 1 cut(s) 1959
FblI GTMKAC 2 cut(s) 47, 1191
FokI GGATG 6 cut(s) 374, 671, 1230, 1481, 2084, 2347
FriOI GRGCYC 4 cut(s) 167, 930, 1065, 1973
FspBI CTAG 6 cut(s) 177, 204, 425, 917, 1974, 2451
GlaI GCGC 2 cut(s) 1180, 1206
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 2 cut(s) 1182, 1208
HaeIII GGCC 7 cut(s) 552, 732, 848, 1063, 1200, 1872, 2032
HapII CCGG 8 cut(s) 589, 991, 1025, 1146, 1182, 1187, 1202, 1212
HgaI GACGC 1 cut(s) 833
HhaI GCGC 2 cut(s) 1181, 1207
Hin1I GRCGYC 3 cut(s) 825, 873, 1179
Hin6I GCGC 2 cut(s) 1179, 1205
HinP1I GCGC 2 cut(s) 1179, 1205
HincII GTYRAC 1 cut(s) 1192
HindII GTYRAC 1 cut(s) 1192
HpaII CCGG 8 cut(s) 589, 991, 1025, 1146, 1182, 1187, 1202, 1212
Hpy166II GTNNAC 5 cut(s) 48, 1030, 1039, 1192, 1884
Hpy188III TCNNGA 9 cut(s) 212, 392, 454, 1519, 1709, 2138, 2176, 2306, 2451
Hpy8I GTNNAC 5 cut(s) 48, 1030, 1039, 1192, 1884
Hpy99I CGWCG 6 cut(s) 731, 827, 875, 974, 1220, 1597
HpyCH4III ACNGT 5 cut(s) 245, 348, 1220, 1798, 1849
HpyCH4IV ACGT 8 cut(s) 717, 807, 873, 1592, 1619, 1988, 2003, 2182
HpyF3I CTNAG 8 cut(s) 20, 455, 535, 900, 1278, 1476, 1710, 2299
HpySE526I ACGT 8 cut(s) 717, 807, 873, 1592, 1619, 1988, 2003, 2182
Hsp92I GRCGYC 3 cut(s) 825, 873, 1179
HspAI GCGC 2 cut(s) 1179, 1205
KasI GGCGCC 1 cut(s) 1178
LmnI GCTCC 4 cut(s) 162, 1897, 1968, 2178
Lsp1109I GCAGC 6 cut(s) 156, 1146, 1761, 1850, 2062, 2137
LweI GCATC 3 cut(s) 1666, 2062, 2369
MabI ACCWGGT 1 cut(s) 1326
MaeI CTAG 6 cut(s) 177, 204, 425, 917, 1974, 2451
MaeII ACGT 8 cut(s) 717, 807, 873, 1592, 1619, 1988, 2003, 2182
MaeIII GTNAC 5 cut(s) 98, 172, 878, 1100, 2256
MbiI CCGCTC 1 cut(s) 961
MboII GAAGA 6 cut(s) 116, 200, 457, 1325, 1660, 2212
MflI RGATCY 4 cut(s) 129, 280, 1094, 2380
MhlI GDGCHC 5 cut(s) 167, 930, 1065, 1550, 1973
Mly113I GGCGCC 1 cut(s) 1179
MlyI GAGTC 7 cut(s) 599, 827, 974, 1097, 1667, 1948, 2014
MmeI TCCRAC 2 cut(s) 624, 1131
MroXI GAANNNNTTC 2 cut(s) 449, 2219
MseI TTAA 2 cut(s) 272, 1581
MslI CAYNNNNRTG 3 cut(s) 396, 1401, 2392
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 8 cut(s) 589, 991, 1025, 1146, 1182, 1187, 1202, 1212
MspR9I CCNGG 7 cut(s) 1113, 1147, 1202, 1328, 1573, 1834, 2348
MvaI CCWGG 5 cut(s) 1113, 1328, 1573, 1834, 2348
NarI GGCGCC 1 cut(s) 1179
NciI CCSGG 2 cut(s) 1147, 1202
NcoI CCATGG 1 cut(s) 292
NdeI CATATG 1 cut(s) 1959
NlaIV GGNNCC 7 cut(s) 260, 1062, 1063, 1064, 1180, 1970, 2382
NmeAIII GCCGAG 1 cut(s) 528
NmuCI GTSAC 5 cut(s) 98, 172, 878, 1100, 2256
NspI RCATGY 3 cut(s) 1400, 1768, 2195
NspV TTCGAA 1 cut(s) 1646
PagI TCATGA 2 cut(s) 391, 2305
PciI ACATGT 2 cut(s) 1396, 2191
PcsI WCGNNNNNNNCGW 3 cut(s) 723, 984, 1547
PdmI GAANNNNTTC 2 cut(s) 449, 2219
PfeI GAWTC 8 cut(s) 71, 113, 377, 401, 450, 1424, 1643, 2141
PflFI GACNNNGTC 2 cut(s) 1147, 1220
PflMI CCANNNNNTGG 2 cut(s) 1916, 2353
PinAI ACCGGT 1 cut(s) 1186
Ple19I CGATCG 1 cut(s) 956
PleI GAGTC 7 cut(s) 599, 827, 974, 1097, 1667, 1948, 2014
PluTI GGCGCC 1 cut(s) 1182
PmaCI CACGTG 1 cut(s) 2004
PmlI CACGTG 1 cut(s) 2004
PpsI GAGTC 7 cut(s) 599, 827, 974, 1097, 1667, 1948, 2014
Ppu21I YACGTR 1 cut(s) 2004
PpuMI RGGWCCY 2 cut(s) 1458, 2295
PscI ACATGT 2 cut(s) 1396, 2191
PshAI GACNNNNGTC 1 cut(s) 876
PshBI ATTAAT 1 cut(s) 1581
PsiI TTATAA 1 cut(s) 1527
Psp124BI GAGCTC 2 cut(s) 167, 930
Psp5II RGGWCCY 2 cut(s) 1458, 2295
Psp6I CCWGG 5 cut(s) 1111, 1326, 1571, 1832, 2346
PspCI CACGTG 1 cut(s) 2004
PspGI CCWGG 5 cut(s) 1111, 1326, 1571, 1832, 2346
PspN4I GGNNCC 7 cut(s) 260, 1062, 1063, 1064, 1180, 1970, 2382
PspOMI GGGCCC 1 cut(s) 1061
PspPPI RGGWCCY 2 cut(s) 1458, 2295
PstNI CAGNNNCTG 2 cut(s) 1676, 2249
PsuI RGATCY 4 cut(s) 129, 280, 1094, 2380
PsyI GACNNNGTC 2 cut(s) 1147, 1220
PvuI CGATCG 1 cut(s) 956
RsaI GTAC 3 cut(s) 2064, 2205, 2228
RsaNI GTAC 3 cut(s) 2063, 2204, 2227
RseI CAYNNNNRTG 3 cut(s) 396, 1401, 2392
Rsr2I CGGWCCG 2 cut(s) 1153, 1184
RsrII CGGWCCG 2 cut(s) 1153, 1184
SacI GAGCTC 2 cut(s) 167, 930
SalI GTCGAC 1 cut(s) 1190
SaqAI TTAA 2 cut(s) 272, 1581
SchI GAGTC 7 cut(s) 599, 827, 974, 1097, 1667, 1948, 2014
ScrFI CCNGG 7 cut(s) 1113, 1147, 1202, 1328, 1573, 1834, 2348
SduI GDGCHC 5 cut(s) 167, 930, 1065, 1550, 1973
SexAI ACCWGGT 1 cut(s) 1326
SfaNI GCATC 3 cut(s) 1666, 2062, 2369
SfcI CTRYAG 1 cut(s) 2127
SfoI GGCGCC 1 cut(s) 1180
SfuI TTCGAA 1 cut(s) 1646
SinI GGWCC 7 cut(s) 1027, 1143, 1153, 1184, 1458, 1830, 2295
SmiMI CAYNNNNRTG 3 cut(s) 396, 1401, 2392
SmlI CTYRAG 3 cut(s) 94, 166, 1901
SmoI CTYRAG 3 cut(s) 94, 166, 1901
SspDI GGCGCC 1 cut(s) 1178
SspMI CTAG 6 cut(s) 177, 204, 425, 917, 1974, 2451
SstI GAGCTC 2 cut(s) 167, 930
StyD4I CCNGG 7 cut(s) 1111, 1145, 1200, 1326, 1571, 1832, 2346
StyI CCWWGG 4 cut(s) 254, 292, 1308, 1973
TaaI ACNGT 5 cut(s) 245, 348, 1220, 1798, 1849
TaiI ACGT 8 cut(s) 720, 810, 876, 1595, 1622, 1991, 2006, 2185
TatI WGTACW 1 cut(s) 2226
TauI GCSGC 5 cut(s) 150, 830, 961, 1256, 1875
TfiI GAWTC 8 cut(s) 71, 113, 377, 401, 450, 1424, 1643, 2141
Tru1I TTAA 2 cut(s) 272, 1581
Tru9I TTAA 2 cut(s) 272, 1581
TscAI CASTG 4 cut(s) 248, 353, 1830, 2424
TseFI GTSAC 5 cut(s) 98, 172, 878, 1100, 2256
TseI GCWGC 6 cut(s) 144, 1134, 1774, 1863, 2075, 2150
Tsp45I GTSAC 5 cut(s) 98, 172, 878, 1100, 2256
TspDTI ATGAA 8 cut(s) 43, 233, 376, 380, 1232, 1548, 1575, 2037
TspGWI ACGGA 2 cut(s) 958, 2382
TspRI CASTG 4 cut(s) 248, 353, 1830, 2424
Tth111I GACNNNGTC 2 cut(s) 1147, 1220
Van91I CCANNNNNTGG 2 cut(s) 1916, 2353
VpaK11BI GGWCC 7 cut(s) 1027, 1143, 1153, 1184, 1458, 1830, 2295
VspI ATTAAT 1 cut(s) 1581
XagI CCTNNNNNAGG 1 cut(s) 202
XapI RAATTY 3 cut(s) 1353, 1372, 2215
XbaI TCTAGA 1 cut(s) 2450
XceI RCATGY 3 cut(s) 1400, 1768, 2195
XcmI CCANNNNNNNNNTGG 1 cut(s) 2391
XmaJI CCTAGG 1 cut(s) 1973
XmiI GTMKAC 2 cut(s) 47, 1191
XmnI GAANNNNTTC 2 cut(s) 449, 2219
XspI CTAG 6 cut(s) 177, 204, 425, 917, 1974, 2451
ZraI GACGTC 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.