Rh2DG631900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012436
Physical Location & Seq
Forward (+)
86106090 .. 86108690
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG631900.1

Sequence Viewer

Length: 2046 bp
ATGACCACCACCATGTCCTCCGCCACGGCCACCACCACTACCCTCCTCCTCCTCCTCCTAACCCTAATCGCCACCTCCACCTCCACCTCAGCCCCAATTCTCGGCCTCGACACCTTCCTCACCCACCAGTCCCGCTACGACCGGCAAGCCTCCAACGACTCCTTCCAGTCTCTCTCCTCCACTCTCCGCCACTCCCTTTCTCTCTCCTCGCCGTCTCTCTCCCACTCTCTCTCCTCCATCCTCTCCCTCACCATCCCTCTCTCTCTCCACGTCCGCCTCGTCGGCCCTTCCTTCCCTTCCTCCTCCGCCACTCTCCTCTCCTCCTTCCTCTCCGCCTCCCAGACCTCCGATCACTTCCACGTCATCACCCCCGCCGACGCCGCCGACTCTCACCGCTTGGCCGTCAAGCACTCCCTCCACCTCGACGTCAGTCACTCCCCCTCCCTCGCCTCTCAGCTCTCCAAAACCCTAGCCTCCGAGCTCGCCAATACCCCCGCCAGTCTCCGATCGCCGCTCGTCTCCGTCGCCTACGACTCAATCGACCGGATCATCAAGCAGGACTTCGAGAAAGAGAAGCCGGTCCACGGCGTTTACCTCTATTTCTTGGATTTGGGGCCCCAATCCAAGAGCTATGCTTACAGCTATGGATCTGGTGACTCCTCTCCTGGCTTCACCAAGTGCTTGGGCAGCGTTTGGACCGGGTCGGACCGCTACATTTGGATCGATTTGGGCGCCGGACCGGTCGACTACGGCCCGGCGCTTTCCGGCGACGGTGTCTTGCCGAAAGGCGAGTTTCATCCTCTGGCGGCGTTGCACGGCTCGCCGAAAGCTCAGAAGGCTTTGCTGGCGGACTTGGCTTCCTTGGTTTGGAGTGCTTACCAGGTTCTTCTTGTTCCCTCTTTGAGAATTCCTGTTCAGTATGAGAATTCTTTGATTGTTCAGTTTATACATGTTTATGGAGGTGAGAGTGGTAAGGATTCGAGTGGTTTGGATTGGAAATCGATTGAGAGGACCTTTATGGATGAGGCTAAGGATAATGGCTTGTTGTTAGGGGATCAGTCTTTGAGTTTCAAGAATTATAAGGTGAAGTATTCGGGGTGCCCGATATGTTCATTTGCTATTGCCAGGTCCATTAATTCATATACGTCGAGGTTCTTGTTTGATAACTATACGTTGATTGCAAGTGAGTATTTGGATTCGAAGAGATTGCATCAGATATTGTCAGACTCTGCTGATGAGTTTAGGAGAGTGGCAGGGTTTCCTGAGGAGGATTTTGGCAGGGTGCTTCCTGTGTATGTGTTTGATTTGGACTACCACATGCTGTTGCTGCTCGATAGGTATCACCAAACTGTTGCTTTTAGAGATATGGTGATTGCAGTGAGGACCAGGAACACACAGACTGTGAGTGATTATAGCTGCAATGGCCGCCATGTGTTTACACAGACGAGGGAGCTTGAGAGACCACTTGTTGGTTCGATTTTACAGAGTATGTGGGGAGTGTCGCCGACTCATATGTTGTGGAGCCCTAGGCACAATACTACGTTGGTGGATTACACGTGGAGTGTTGGGCAGACTCCATTTGGGCCGTTCTCGGAGATTTCATCACTATCATTCGTACAGAAGGATGCTGCTCGGAGAAATGTTCTTTTGACATCACTAAATTACAGCATATCAAGTGCTGTAGATGTTCTTGAATCCATAGCTGCACATGGTGGGGAGAGAAAGCTCCTGAAACGTAATCGACATGTTGAGTTCGTACAAAGGTGGAATTTCTTCAAGTACAAGCTGGACAAAGCAGTTTCTGCAATGTCACATTTAGACTCTGAGATGGCTTTATACTATATGAGGTCCTCAGATCATGATCTATATGCTATCCACTCTATTGTGTATCATGCCTCCCAGGAATTGGAGGCATCCCTTGTATGTTTCAAGGATCCACCATTTCCATGGTCTTTTGTGTTTATGGTTGCAGTGCTTCTCCTTCTTGGCTATTATATTAGTTCTAGAAAACACAAACTATTCAGAAGTAAGAGTAAGCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

681

Amino Acids

75.77

Weight (kDa)

7.41

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 86 - 299 5.3e-88 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1080
AatII GACGTC 1 cut(s) 429
AccB1I GGYRCC 2 cut(s) 731, 1098
AccB7I CCANNNNNTGG 2 cut(s) 1469, 1906
AccBSI CCGCTC 1 cut(s) 514
AccI GTMKAC 1 cut(s) 744
AclWI GGATC 6 cut(s) 554, 655, 728, 1062, 1928, 1941
AcoI YGGCCR 3 cut(s) 27, 399, 1423
AcsI RAATTY 3 cut(s) 906, 925, 1768
AcvI CACGTG 1 cut(s) 1557
AcyI GRCGYC 3 cut(s) 378, 426, 732
AdeI CACNNNGTG 2 cut(s) 678, 1712
AfaI GTAC 3 cut(s) 1617, 1758, 1781
AfiI CCNNNNNNNGG 5 cut(s) 101, 584, 867, 1469, 1906
AflIII ACRYGT 3 cut(s) 949, 1554, 1744
AgeI ACCGGT 1 cut(s) 739
AgsI TTSAA 4 cut(s) 1072, 1694, 1777, 1930
AjiI CACGTC 2 cut(s) 271, 361
AjnI CCWGG 5 cut(s) 664, 879, 1124, 1385, 1899
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 5 cut(s) 174, 219, 506, 523, 1453
AlwI GGATC 6 cut(s) 554, 655, 728, 1062, 1928, 1941
AlwNI CAGNNNCTG 2 cut(s) 1229, 1802
AoxI GGCC 8 cut(s) 27, 103, 283, 399, 614, 751, 1423, 1583
ApaI GGGCCC 1 cut(s) 618
ApeKI GCWGC 5 cut(s) 687, 1327, 1416, 1628, 1703
ApoI RAATTY 3 cut(s) 906, 925, 1768
AseI ATTAAT 1 cut(s) 1134
AsiGI ACCGGT 1 cut(s) 739
Asp700I GAANNNNTTC 1 cut(s) 1772
AspA2I CCTAGG 1 cut(s) 1526
AspLEI GCGC 2 cut(s) 734, 760
AsuC2I CCSGG 2 cut(s) 700, 755
AsuII TTCGAA 1 cut(s) 1199
AvaII GGWCC 8 cut(s) 580, 696, 706, 737, 1011, 1128, 1383, 1848
AvrII CCTAGG 1 cut(s) 1526
AxyI CCTNAGG 1 cut(s) 1263
BaeGI GKGCMC 2 cut(s) 618, 1103
BamHI GGATCC 1 cut(s) 1933
BanI GGYRCC 2 cut(s) 731, 1098
BanII GRGCYC 3 cut(s) 483, 618, 1526
BbrPI CACGTG 1 cut(s) 1557
Bbv12I GWGCWC 1 cut(s) 483
BbvCI CCTCAGC 1 cut(s) 88
BbvI GCAGC 5 cut(s) 699, 1314, 1403, 1615, 1690
BccI CCATC 3 cut(s) 245, 260, 1822
BceAI ACGGC 7 cut(s) 42, 196, 386, 601, 766, 832, 1570
BcgI CGANNNNNNTGC 2 cut(s) 1189, 1223
BciT130I CCWGG 5 cut(s) 666, 881, 1126, 1387, 1901
BcnI CCSGG 2 cut(s) 700, 755
BcoDI GTCTC 5 cut(s) 174, 219, 506, 523, 1453
BfaI CTAG 3 cut(s) 470, 1527, 2004
BfmI CTRYAG 1 cut(s) 1680
BfoI RGCGCY 2 cut(s) 735, 761
BglI GCCNNNNNGGC 1 cut(s) 282
BisI GCNGC 9 cut(s) 381, 512, 688, 807, 1328, 1417, 1426, 1629, 1704
BlnI CCTAGG 1 cut(s) 1526
BlsI GCNGC 9 cut(s) 382, 513, 689, 808, 1329, 1418, 1427, 1630, 1705
Bme1390I CCNGG 7 cut(s) 666, 700, 755, 881, 1126, 1387, 1901
Bme18I GGWCC 8 cut(s) 580, 696, 706, 737, 1011, 1128, 1383, 1848
BmgBI CACGTC 2 cut(s) 271, 361
BmiI GGNNCC 7 cut(s) 615, 616, 617, 733, 1100, 1523, 1935
BmrFI CCNGG 7 cut(s) 666, 700, 755, 881, 1126, 1387, 1901
BmsI GCATC 3 cut(s) 1219, 1615, 1922
BoxI GACNNNNGTC 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 88, 1029
Bpu14I TTCGAA 1 cut(s) 1199
BpuEI CTTGAG 1 cut(s) 1475
BpuMI CCSGG 2 cut(s) 700, 755
Bsa29I ATCGAT 2 cut(s) 723, 1001
BsaAI YACGTR 1 cut(s) 1557
BsaBI GATNNNNATC 2 cut(s) 1338, 1860
BsaHI GRCGYC 3 cut(s) 378, 426, 732
BsaI GGTCTC 1 cut(s) 1453
BsaJI CCNNGG 6 cut(s) 24, 583, 861, 1526, 1899, 1946
BsaWI WCCGGW 2 cut(s) 543, 739
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 425, 455
Bsc4I CCNNNNNNNGG 5 cut(s) 101, 584, 867, 1469, 1906
Bse118I RCCGGY 3 cut(s) 141, 577, 739
Bse1I ACTGG 3 cut(s) 127, 166, 498
Bse21I CCTNAGG 1 cut(s) 1263
Bse3DI GCAATG 2 cut(s) 1426, 1812
Bse8I GATNNNNATC 2 cut(s) 1338, 1860
BseBI CCWGG 5 cut(s) 666, 881, 1126, 1387, 1901
BseCI ATCGAT 2 cut(s) 723, 1001
BseDI CCNNGG 6 cut(s) 24, 583, 861, 1526, 1899, 1946
BseGI GGATG 6 cut(s) 237, 252, 796, 1027, 1630, 1913
BseJI GATNNNNATC 2 cut(s) 1338, 1860
BseLI CCNNNNNNNGG 5 cut(s) 101, 584, 867, 1469, 1906
BseMI GCAATG 2 cut(s) 1426, 1812
BseMII CTCAG 6 cut(s) 102, 467, 845, 1254, 1815, 1866
BseNI ACTGG 3 cut(s) 127, 166, 498
BseSI GKGCMC 2 cut(s) 618, 1103
BseXI GCAGC 5 cut(s) 699, 1314, 1403, 1615, 1690
BsgI GTGCAG 1 cut(s) 1689
Bsh1285I CGRYCG 4 cut(s) 142, 509, 544, 744
BshFI GGCC 8 cut(s) 29, 105, 285, 401, 616, 753, 1425, 1585
BshNI GGYRCC 2 cut(s) 731, 1098
BshTI ACCGGT 1 cut(s) 739
BshVI ATCGAT 2 cut(s) 723, 1001
BsiEI CGRYCG 4 cut(s) 142, 509, 544, 744
BsiHKAI GWGCWC 1 cut(s) 483
BsiSI CCGG 8 cut(s) 142, 544, 578, 699, 735, 740, 755, 765
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 5 cut(s) 101, 584, 867, 1469, 1906
BsmAI GTCTC 5 cut(s) 174, 219, 506, 523, 1453
BsmBI CGTCTC 2 cut(s) 219, 523
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 8 cut(s) 29, 105, 285, 401, 616, 753, 1425, 1585
Bso31I GGTCTC 1 cut(s) 1453
Bsp119I TTCGAA 1 cut(s) 1199
Bsp120I GGGCCC 1 cut(s) 614
Bsp1286I GDGCHC 4 cut(s) 483, 618, 1103, 1526
Bsp143I GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
Bsp19I CCATGG 1 cut(s) 1946
BspANI GGCC 8 cut(s) 29, 105, 285, 401, 616, 753, 1425, 1585
BspCNI CTCAG 6 cut(s) 101, 466, 844, 1255, 1816, 1865
BspDI ATCGAT 2 cut(s) 723, 1001
BspHI TCATGA 1 cut(s) 1858
BspLI GGNNCC 7 cut(s) 615, 616, 617, 733, 1100, 1523, 1935
BspPI GGATC 6 cut(s) 554, 655, 728, 1062, 1928, 1941
BspT104I TTCGAA 1 cut(s) 1199
BspT107I GGYRCC 2 cut(s) 731, 1098
BspTNI GGTCTC 1 cut(s) 1453
BsrBI CCGCTC 1 cut(s) 514
BsrDI GCAATG 2 cut(s) 1426, 1812
BsrFI RCCGGY 3 cut(s) 141, 577, 739
BsrI ACTGG 3 cut(s) 127, 166, 498
BssAI RCCGGY 3 cut(s) 141, 577, 739
BssECI CCNNGG 6 cut(s) 24, 583, 861, 1526, 1899, 1946
BssMI GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
BssNI GRCGYC 3 cut(s) 378, 426, 732
BssT1I CCWWGG 3 cut(s) 861, 1526, 1946
Bst2UI CCWGG 5 cut(s) 666, 881, 1126, 1387, 1901
Bst4CI ACNGT 3 cut(s) 773, 1351, 1402
Bst6I CTCTTC 1 cut(s) 1196
BstACI GRCGYC 3 cut(s) 378, 426, 732
BstAPI GCANNNNNTGC 1 cut(s) 1802
BstBAI YACGTR 1 cut(s) 1557
BstBI TTCGAA 1 cut(s) 1199
BstC8I GCNNGC 4 cut(s) 147, 483, 821, 846
BstDEI CTNAG 7 cut(s) 88, 453, 831, 1029, 1263, 1824, 1852
BstDSI CCRYGG 3 cut(s) 24, 583, 1946
BstF5I GGATG 6 cut(s) 237, 252, 796, 1027, 1630, 1913
BstH2I RGCGCY 2 cut(s) 735, 761
BstHHI GCGC 2 cut(s) 734, 760
BstKTI GATC 9 cut(s) 352, 509, 549, 650, 723, 1057, 1858, 1864, 1936
BstMAI GTCTC 5 cut(s) 174, 219, 506, 523, 1453
BstMBI GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
BstMCI CGRYCG 4 cut(s) 142, 509, 544, 744
BstNI CCWGG 5 cut(s) 666, 881, 1126, 1387, 1901
BstNSI RCATGY 3 cut(s) 953, 1321, 1748
BstPAI GACNNNNGTC 1 cut(s) 429
BstSCI CCNGG 7 cut(s) 664, 698, 753, 879, 1124, 1385, 1899
BstSFI CTRYAG 1 cut(s) 1680
BstSLI GKGCMC 2 cut(s) 618, 1103
BstV1I GCAGC 5 cut(s) 699, 1314, 1403, 1615, 1690
BstX2I RGATCY 2 cut(s) 647, 1933
BstXI CCANNNNNNTGG 2 cut(s) 682, 1947
BstYI RGATCY 2 cut(s) 647, 1933
Bsu15I ATCGAT 2 cut(s) 723, 1001
Bsu36I CCTNAGG 1 cut(s) 1263
BsuRI GGCC 8 cut(s) 29, 105, 285, 401, 616, 753, 1425, 1585
BsuTUI ATCGAT 2 cut(s) 723, 1001
BtgI CCRYGG 3 cut(s) 24, 583, 1946
BtrI CACGTC 2 cut(s) 271, 361
BtsCI GGATG 6 cut(s) 237, 252, 796, 1027, 1630, 1913
BtsI GCAGTG 2 cut(s) 1383, 1977
BtsIMutI CAGTG 2 cut(s) 1383, 1977
Cac8I GCNNGC 4 cut(s) 147, 483, 821, 846
CaiI CAGNNNCTG 2 cut(s) 1229, 1802
CciI TCATGA 1 cut(s) 1858
CfoI GCGC 2 cut(s) 734, 760
Cfr10I RCCGGY 3 cut(s) 141, 577, 739
ClaI ATCGAT 2 cut(s) 723, 1001
CpoI CGGWCCG 2 cut(s) 706, 737
CseI GACGC 1 cut(s) 386
CsiI ACCWGGT 1 cut(s) 879
Csp6I GTAC 3 cut(s) 1616, 1757, 1780
CspAI ACCGGT 1 cut(s) 739
CspI CGGWCCG 2 cut(s) 706, 737
CviAII CATG 9 cut(s) 13, 950, 1318, 1430, 1709, 1745, 1859, 1892, 1947
CviQI GTAC 3 cut(s) 1616, 1757, 1780
DdeI CTNAG 7 cut(s) 88, 453, 831, 1029, 1263, 1824, 1852
DinI GGCGCC 1 cut(s) 733
DpnI GATC 9 cut(s) 351, 508, 548, 649, 722, 1056, 1857, 1863, 1935
DpnII GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
DraIII CACNNNGTG 2 cut(s) 678, 1712
EaeI YGGCCR 3 cut(s) 27, 399, 1423
Eam1104I CTCTTC 1 cut(s) 1196
EarI CTCTTC 1 cut(s) 1196
EciI GGCGGA 6 cut(s) 10, 176, 263, 295, 322, 863
Ecl136II GAGCTC 1 cut(s) 481
Eco130I CCWWGG 3 cut(s) 861, 1526, 1946
Eco24I GRGCYC 3 cut(s) 483, 618, 1526
Eco31I GGTCTC 1 cut(s) 1453
Eco47I GGWCC 8 cut(s) 580, 696, 706, 737, 1011, 1128, 1383, 1848
Eco53kI GAGCTC 1 cut(s) 481
Eco72I CACGTG 1 cut(s) 1557
Eco81I CCTNAGG 1 cut(s) 1263
EcoICRI GAGCTC 1 cut(s) 481
EcoO109I RGGNCCY 4 cut(s) 614, 615, 1011, 1848
EcoRI GAATTC 2 cut(s) 906, 925
EcoRII CCWGG 5 cut(s) 664, 879, 1124, 1385, 1899
EcoT14I CCWWGG 3 cut(s) 861, 1526, 1946
EcoT38I GRGCYC 3 cut(s) 483, 618, 1526
EgeI GGCGCC 1 cut(s) 733
EheI GGCGCC 1 cut(s) 733
ErhI CCWWGG 3 cut(s) 861, 1526, 1946
Esp3I CGTCTC 2 cut(s) 219, 523
FaeI CATG 9 cut(s) 16, 953, 1321, 1433, 1712, 1748, 1862, 1895, 1950
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FaqI GGGAC 1 cut(s) 115
FatI CATG 9 cut(s) 12, 949, 1317, 1429, 1708, 1744, 1858, 1891, 1946
FauI CCCGC 3 cut(s) 140, 379, 502
FauNDI CATATG 1 cut(s) 1512
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 9 cut(s) 381, 512, 688, 807, 1328, 1417, 1426, 1629, 1704
FokI GGATG 6 cut(s) 224, 239, 783, 1034, 1637, 1900
FriOI GRGCYC 3 cut(s) 483, 618, 1526
Fsp4HI GCNGC 9 cut(s) 381, 512, 688, 807, 1328, 1417, 1426, 1629, 1704
FspBI CTAG 3 cut(s) 470, 1527, 2004
GlaI GCGC 2 cut(s) 733, 759
GluI GCNGC 9 cut(s) 381, 512, 688, 807, 1328, 1417, 1426, 1629, 1704
HaeII RGCGCY 2 cut(s) 735, 761
HaeIII GGCC 8 cut(s) 29, 105, 285, 401, 616, 753, 1425, 1585
HapII CCGG 8 cut(s) 142, 544, 578, 699, 735, 740, 755, 765
HgaI GACGC 1 cut(s) 386
HhaI GCGC 2 cut(s) 734, 760
Hin1I GRCGYC 3 cut(s) 378, 426, 732
Hin1II CATG 9 cut(s) 16, 953, 1321, 1433, 1712, 1748, 1862, 1895, 1950
Hin6I GCGC 2 cut(s) 732, 758
HinP1I GCGC 2 cut(s) 732, 758
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HpaII CCGG 8 cut(s) 142, 544, 578, 699, 735, 740, 755, 765
Hpy166II GTNNAC 4 cut(s) 583, 592, 745, 1437
Hpy188III TCNNGA 7 cut(s) 565, 1072, 1262, 1691, 1729, 1859, 2004
Hpy8I GTNNAC 4 cut(s) 583, 592, 745, 1437
Hpy99I CGWCG 6 cut(s) 284, 380, 428, 527, 773, 1150
HpyAV CCTTC 9 cut(s) 124, 172, 297, 301, 306, 334, 829, 1615, 1991
HpyCH4III ACNGT 3 cut(s) 773, 1351, 1402
HpyCH4IV ACGT 8 cut(s) 270, 360, 426, 1145, 1172, 1541, 1556, 1735
HpyCH4V TGCA 8 cut(s) 814, 1181, 1210, 1376, 1419, 1706, 1805, 1970
HpyF3I CTNAG 7 cut(s) 88, 453, 831, 1029, 1263, 1824, 1852
HpySE526I ACGT 8 cut(s) 270, 360, 426, 1145, 1172, 1541, 1556, 1735
Hsp92I GRCGYC 3 cut(s) 378, 426, 732
Hsp92II CATG 9 cut(s) 16, 953, 1321, 1433, 1712, 1748, 1862, 1895, 1950
HspAI GCGC 2 cut(s) 732, 758
KasI GGCGCC 1 cut(s) 731
Kzo9I GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
LmnI GCTCC 3 cut(s) 1450, 1521, 1731
Lsp1109I GCAGC 5 cut(s) 699, 1314, 1403, 1615, 1690
LweI GCATC 3 cut(s) 1219, 1615, 1922
MabI ACCWGGT 1 cut(s) 879
MaeI CTAG 3 cut(s) 470, 1527, 2004
MaeII ACGT 8 cut(s) 270, 360, 426, 1145, 1172, 1541, 1556, 1735
MaeIII GTNAC 3 cut(s) 431, 653, 1809
MalI GATC 9 cut(s) 351, 508, 548, 649, 722, 1056, 1857, 1863, 1935
MbiI CCGCTC 1 cut(s) 514
MboI GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
MboII GAAGA 3 cut(s) 878, 1213, 1765
MflI RGATCY 2 cut(s) 647, 1933
MhlI GDGCHC 4 cut(s) 483, 618, 1103, 1526
MluCI AATT 9 cut(s) 96, 906, 925, 1075, 1135, 1660, 1768, 1904, 2039
Mly113I GGCGCC 1 cut(s) 732
MlyI GAGTC 8 cut(s) 152, 380, 527, 650, 1220, 1501, 1567, 1814
MmeI TCCRAC 2 cut(s) 177, 684
MroXI GAANNNNTTC 1 cut(s) 1772
MseI TTAA 1 cut(s) 1134
MslI CAYNNNNRTG 3 cut(s) 11, 954, 1945
MspI CCGG 8 cut(s) 142, 544, 578, 699, 735, 740, 755, 765
MspR9I CCNGG 7 cut(s) 666, 700, 755, 881, 1126, 1387, 1901
MvaI CCWGG 5 cut(s) 666, 881, 1126, 1387, 1901
NarI GGCGCC 1 cut(s) 732
NciI CCSGG 2 cut(s) 700, 755
NcoI CCATGG 1 cut(s) 1946
NdeI CATATG 1 cut(s) 1512
NdeII GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
NlaIII CATG 9 cut(s) 16, 953, 1321, 1433, 1712, 1748, 1862, 1895, 1950
NlaIV GGNNCC 7 cut(s) 615, 616, 617, 733, 1100, 1523, 1935
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 3 cut(s) 431, 653, 1809
NspI RCATGY 3 cut(s) 953, 1321, 1748
NspV TTCGAA 1 cut(s) 1199
PagI TCATGA 1 cut(s) 1858
PciI ACATGT 2 cut(s) 949, 1744
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 537, 1100
PdmI GAANNNNTTC 1 cut(s) 1772
PfeI GAWTC 3 cut(s) 977, 1196, 1694
PflFI GACNNNGTC 2 cut(s) 700, 773
PflMI CCANNNNNTGG 2 cut(s) 1469, 1906
PinAI ACCGGT 1 cut(s) 739
PkrI GCNGC 9 cut(s) 382, 513, 689, 808, 1329, 1418, 1427, 1630, 1705
Ple19I CGATCG 1 cut(s) 509
PleI GAGTC 8 cut(s) 152, 380, 527, 650, 1220, 1501, 1567, 1814
PluTI GGCGCC 1 cut(s) 735
PmaCI CACGTG 1 cut(s) 1557
PmlI CACGTG 1 cut(s) 1557
PpsI GAGTC 8 cut(s) 152, 380, 527, 650, 1220, 1501, 1567, 1814
Ppu21I YACGTR 1 cut(s) 1557
PpuMI RGGWCCY 2 cut(s) 1011, 1848
PscI ACATGT 2 cut(s) 949, 1744
PshAI GACNNNNGTC 1 cut(s) 429
PshBI ATTAAT 1 cut(s) 1134
PsiI TTATAA 1 cut(s) 1080
Psp124BI GAGCTC 1 cut(s) 483
Psp5II RGGWCCY 2 cut(s) 1011, 1848
Psp6I CCWGG 5 cut(s) 664, 879, 1124, 1385, 1899
PspCI CACGTG 1 cut(s) 1557
PspGI CCWGG 5 cut(s) 664, 879, 1124, 1385, 1899
PspN4I GGNNCC 7 cut(s) 615, 616, 617, 733, 1100, 1523, 1935
PspOMI GGGCCC 1 cut(s) 614
PspPPI RGGWCCY 2 cut(s) 1011, 1848
PstNI CAGNNNCTG 2 cut(s) 1229, 1802
PsuI RGATCY 2 cut(s) 647, 1933
PsyI GACNNNGTC 2 cut(s) 700, 773
PvuI CGATCG 1 cut(s) 509
RsaI GTAC 3 cut(s) 1617, 1758, 1781
RsaNI GTAC 3 cut(s) 1616, 1757, 1780
RseI CAYNNNNRTG 3 cut(s) 11, 954, 1945
Rsr2I CGGWCCG 2 cut(s) 706, 737
RsrII CGGWCCG 2 cut(s) 706, 737
SacI GAGCTC 1 cut(s) 483
SalI GTCGAC 1 cut(s) 743
SaqAI TTAA 1 cut(s) 1134
SatI GCNGC 9 cut(s) 381, 512, 688, 807, 1328, 1417, 1426, 1629, 1704
Sau3AI GATC 9 cut(s) 349, 506, 546, 647, 720, 1054, 1855, 1861, 1933
SchI GAGTC 8 cut(s) 152, 380, 527, 650, 1220, 1501, 1567, 1814
ScrFI CCNGG 7 cut(s) 666, 700, 755, 881, 1126, 1387, 1901
SduI GDGCHC 4 cut(s) 483, 618, 1103, 1526
SexAI ACCWGGT 1 cut(s) 879
SfaNI GCATC 3 cut(s) 1219, 1615, 1922
SfcI CTRYAG 1 cut(s) 1680
SfoI GGCGCC 1 cut(s) 733
SfuI TTCGAA 1 cut(s) 1199
SinI GGWCC 8 cut(s) 580, 696, 706, 737, 1011, 1128, 1383, 1848
SmiMI CAYNNNNRTG 3 cut(s) 11, 954, 1945
SmlI CTYRAG 1 cut(s) 1454
SmoI CTYRAG 1 cut(s) 1454
Sse9I AATT 9 cut(s) 96, 906, 925, 1075, 1135, 1660, 1768, 1904, 2039
SspDI GGCGCC 1 cut(s) 731
SspMI CTAG 3 cut(s) 470, 1527, 2004
SstI GAGCTC 1 cut(s) 483
StyD4I CCNGG 7 cut(s) 664, 698, 753, 879, 1124, 1385, 1899
StyI CCWWGG 3 cut(s) 861, 1526, 1946
TaaI ACNGT 3 cut(s) 773, 1351, 1402
TaiI ACGT 8 cut(s) 273, 363, 429, 1148, 1175, 1544, 1559, 1738
TasI AATT 9 cut(s) 96, 906, 925, 1075, 1135, 1660, 1768, 1904, 2039
TatI WGTACW 1 cut(s) 1779
TauI GCSGC 4 cut(s) 383, 514, 809, 1428
TfiI GAWTC 3 cut(s) 977, 1196, 1694
Tru1I TTAA 1 cut(s) 1134
Tru9I TTAA 1 cut(s) 1134
TscAI CASTG 2 cut(s) 1383, 1977
TseFI GTSAC 3 cut(s) 431, 653, 1809
TseI GCWGC 5 cut(s) 687, 1327, 1416, 1628, 1703
Tsp45I GTSAC 3 cut(s) 431, 653, 1809
TspDTI ATGAA 4 cut(s) 785, 1101, 1128, 1590
TspGWI ACGGA 1 cut(s) 511
TspRI CASTG 2 cut(s) 1383, 1977
Tth111I GACNNNGTC 2 cut(s) 700, 773
Van91I CCANNNNNTGG 2 cut(s) 1469, 1906
VpaK11BI GGWCC 8 cut(s) 580, 696, 706, 737, 1011, 1128, 1383, 1848
VspI ATTAAT 1 cut(s) 1134
XapI RAATTY 3 cut(s) 906, 925, 1768
XbaI TCTAGA 1 cut(s) 2003
XceI RCATGY 3 cut(s) 953, 1321, 1748
XcmI CCANNNNNNNNNTGG 1 cut(s) 1944
XmaJI CCTAGG 1 cut(s) 1526
XmiI GTMKAC 1 cut(s) 744
XmnI GAANNNNTTC 1 cut(s) 1772
XspI CTAG 3 cut(s) 470, 1527, 2004
ZraI GACGTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.