AT3G42660

Minichromosome loss protein, Mcl1, middle region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005318
Physical Location & Seq
Forward (+)
14751280 .. 14755701
4422 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G42660.1

Sequence Viewer

Length: 2862 bp
ATGAAATCACGATCTCTAAAGCTCCGGGAAGCCCATAAAGTCGGCGGAAGCGCCGCCTTCTGCTCTATCCTCTGGGACCACAAAGCCGAACATTTCGTCACTTCATCTTCTTCGGATCCCTCAATCTCCGTCCACGATGGACTCTCAACCTCCACATTACCCCCGACGATTCTCCGTCATCACCAAGACGGCGTCACCTCCTTGGCTCTTAGCAACGACTCTACTCTCCTCGCCTCTGGATCTATCGATCATTGCGTCAAGCTCTACAAGTTCCCAAGTGGGGAATTTCAGACGAACATTACTAGATTTACACTTCCGATTCGCGTGCTTGCGTTTAACGGCTCAGGGAGTCTATTAGCAGCTGCAGGAGATGATGAAGGAATCAAACTTATCAATACATTTGATGGTTCGATTGTTAGAGTTTTGAAAGGGCACAAAGGACCAGTGACCGGCTTAGATTTCCATCCTAATGGTGAGCTTTTAGCTTCGATTGACACAACTGGTACTGTGTTATGCTGGGAACTTCAAAACGGAGTCGTTTCGTTTACCCTTAAGGGTGTTGCGCCAGATACCGGTTTCAACACTTCCATTGTGAATATTCCTAGATGGAGCCCTGATGGAAGAACTCTCGCTGTGCCTGGTTTGAGAAACGATGTTGTGATGTATGATAGATTCACTGGGGAGAAGCTTTTTGCCTTGAGAGGTGATCATCTCGAGGCTATATGTTATTTAACGTGGGCTCCTAATGGCAAGTATATAGCTACATCTGGTCTTGATAAACAAGTGCTTCTTTGGGATGTTGATAAGAAACAAGACATAGATAGGCATAAATTTGAGGAGAGGATATGTTGTATGTCGTGGAAACCGAATGGTAATGCATTGTCGGTTATCGATGCCAAGGGAAGATATGGAGTTTGGGAATCTTTGGTTCCATCATCTATGCTATCTCCTACAGTTGGTGTTCCAGATATAGTACCAAAGAAGAGGAATGAGATTCTTGATTTTGATGATGAAGTTGAGGAAGAAATTTATCGTGCATCTGAAAGTTTAGATGATGCTATGGGAGATAGTGATGATGGAGAATCTCATCATACTAGTAGGAAAAGATTGAGGAAGAAAACTTTGATTGATGAAGATGTTGATGATGCATATGAGGAACTTAATGATGGGAGTAGCTTACCTTCGGCATCTGAGTACAGGAAAAAGTCACATAGAGGACATAGAGAGAAGCAGGGTGCAAGAAGTGGGGCTTTTAAAGGCATTTCAGCCTCAACCAAGTACAAAATGCAAAGTTCCTTTCAACCTGGTGCAACACCACCGGAACCAGGAAAACGGACATTCTTGTGTTACAATATGCTGGGATGTATAACTACTATTGAACATGAGGGGAACTCACGTATTGAGACAGATTTTCATGATACTGGAAGAGGTCCACGAGTTTCATCGATGATAGACATTTACGGCTTCACTATGGCATCAATAAATGAAACTGGGTGTGTCTTTGCAAATCCCTGTAAGGGAGAAAAGAACATGAGTGTTCTGATGTATCGACCATTTAGAAGCTGGGCCAGTAATAGTGAGTGGACAATGAGATTTGAAGGTGAAGAGGTGAAAGTTGTTGCTAACGGTTCTGGTTGGGTTGCTGCAGTTACAAGCCTCAATTTGCTTCGTGTATTTAGCGAGGGCGGTTTACAGAAACATATTCTTTCTCTTGATGGGCCCGTAGTCACTGCAGTAGGATGCAAGGATCATCTAGCTGTGGTAACTCATGTTTCAGACTGTCTTCCTTCAAATGAACAGGTGATGGAATTTAGAGTCTTTAACATATCCAAAATGACTCAGGAGTTGAAAGGCCGTGTTGCCTTAACCCCTGGCTCACGTTTAACATGGATAGGATTCAGTGAAGAAGGTTCTTTGAGCTCATATGATTCTGAGGGTGTGTTGAGGGTTTTTACAAGTCAATATGGTGGCTCATGGATCCCAGTTTTCAGTACCAGTAAAGAGAAGAAACAAGAAGAAAACTACTGGGTGGTTGGCTTGAATACAAGCAGTTTGTACTGTATAGCTTGCAAATACGCGGAGATGTTCCCACAGGTGACACCAAAACCAATCCTTACCATACTAGACCTTTCTCTCCCGCTTGCATCCTCAGATTTAGGAGCAGCATCTCTAGAAAATGAGTTAATACTAAAGCAGCTGCGTTTATACGAGACTCAACGAAAAGTGGACGATATGGCTTTGGTCGGGGTAGACACAACAGCACTTGAAGATGAAGCTTTTGACCTGGAAGTTTCTCAAGATAAATGCATACTAAGGCTCATCAGCTCGTGTTGTAGTAGTGATAGTTTTGCAAGGGCCAGTGAACTTATGGAGCTTTTAACTCTAGAAAAATCAATGAGAGCTGCAATAACACTGGTTACCAAACTCAAGCTTCCTTTTTTGGCAGAGAAATTCAGCAGCATACTCGAGGAAAGGTTGCTTGAAGAAGCTAGCGAGGCGGCGGTAACAAATCCTGCGCTGAATCCAAACGGAGAAGTAGTTACAAGAGTTGAATCCAAGGTCCAGAATCCTCCAGCTTCGATTCAGACTAGTGAAAACACAGAAGCAGTTATGAAATCTTCTGCAACTAAGCTATCTGCTCCTACACTTCTAAAGAAATCGAAAGTTTCAGAAGGTCTAAAGCTGGGAAAAGAACAAACAAAGAAAGACAAAAGTGACGATGCGAAGATAAAAGAGATAAAGAAATTGAATTTGAAGAATCCGGTGAACAATGTTAACAAGGAAGACAAGGGACAGGAAAAGGAAGTGAATCAAGGAGAAGCTCGACGTTCTTCAAACCCGTTCCTCAAATCAACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

953

Amino Acids

104.85

Weight (kDa)

5.95

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 18 - 163 3e-20 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 18 - 140 3.3e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 21 - 92 9.2e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 22 - 305 3.5e-62 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 28 - 179 6.9e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 30 - 184 1.1e-24 WDR5 beta-propeller domain
WD40 PF00400 56 - 89 8.1e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 58 - 185 5.7e-18 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 60 - 179 3.7e-11 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 61 - 184 2.4e-07 WDR55
WD40_Prp19 PF24814 61 - 298 2.1e-26 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 62 - 174 1.7e-07 WDR3 second beta-propeller domain
Beta-prop_WDR19_1st PF23389 114 - 246 1.1e-06 WDR19 first beta-propeller
WD40 PF00400 137 - 173 6.6e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 196 - 306 1.7e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 198 - 294 1.6e-09 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 202 - 300 1.1e-11 THOC3 beta-propeller domain
ANAPC4_WD40 PF12894 216 - 288 7.7e-06 Anaphase-promoting complex subunit 4 WD40 domain
Mcl1_mid PF12341 432 - 714 5.8e-92 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 754 - 826 2.5e-09 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 2250
AccII CGCG 2 cut(s) 324, 2078
AciI CCGC 7 cut(s) 45, 54, 1686, 2078, 2138, 2498, 2501
AclWI GGATC 6 cut(s) 110, 123, 247, 1755, 1972, 1985
AcsI RAATTY 6 cut(s) 284, 830, 1026, 1808, 2450, 2749
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 6 cut(s) 505, 975, 1196, 1280, 1993, 2057
AfiI CCNNNNNNNGG 6 cut(s) 280, 449, 572, 956, 1325, 1517
AflII CTTAAG 1 cut(s) 551
AgeI ACCGGT 1 cut(s) 572
AhlI ACTAGT 2 cut(s) 1094, 2588
AjnI CCWGG 5 cut(s) 637, 1303, 1324, 1870, 2283
Alw21I GWGCWC 1 cut(s) 1922
Alw26I GTCTC 2 cut(s) 1397, 2204
AlwI GGATC 6 cut(s) 110, 123, 247, 1755, 1972, 1985
Ama87I CYCGRG 2 cut(s) 713, 2465
AoxI GGCC 4 cut(s) 1566, 1718, 1852, 2355
ApaI GGGCCC 1 cut(s) 1722
ApeKI GCWGC 8 cut(s) 359, 362, 1643, 2162, 2194, 2197, 2402, 2457
ApoI RAATTY 6 cut(s) 284, 830, 1026, 1808, 2450, 2749
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AsiGI ACCGGT 1 cut(s) 572
AspLEI GCGC 3 cut(s) 53, 565, 2518
AspS9I GGNCC 8 cut(s) 76, 440, 1430, 1566, 1718, 1719, 2355, 2560
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 9 cut(s) 173, 187, 485, 716, 1613, 1621, 1813, 2107, 2776
AsuNHI GCTAGC 1 cut(s) 2489
AvaI CYCGRG 2 cut(s) 713, 2465
AvaII GGWCC 4 cut(s) 76, 440, 1430, 2560
BaeGI GKGCMC 2 cut(s) 435, 1722
BamHI GGATCC 2 cut(s) 115, 1977
BanII GRGCYC 4 cut(s) 614, 742, 1722, 1922
BarI GAAGNNNNNNTAC 2 cut(s) 1165, 1197
BauI CACGAG 2 cut(s) 1434, 2326
BbsI GAAGAC 2 cut(s) 1775, 2790
Bbv12I GWGCWC 1 cut(s) 1922
BbvI GCAGC 8 cut(s) 349, 371, 1630, 2174, 2184, 2206, 2389, 2469
BceAI ACGGC 4 cut(s) 205, 355, 1477, 1839
BcgI CGANNNNNNTGC 4 cut(s) 307, 341, 2446, 2480
BciT130I CCWGG 5 cut(s) 639, 1305, 1326, 1872, 2285
BclI TGATCA 1 cut(s) 706
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 2 cut(s) 1397, 2204
BcuI ACTAGT 2 cut(s) 1094, 2588
BfaI CTAG 9 cut(s) 303, 603, 1095, 1754, 2123, 2171, 2384, 2490, 2589
BfmI CTRYAG 4 cut(s) 363, 951, 1644, 1731
BfoI RGCGCY 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 551
Bme1390I CCNGG 6 cut(s) 26, 639, 1305, 1326, 1872, 2285
Bme18I GGWCC 4 cut(s) 76, 440, 1430, 2560
BmeT110I CYCGRG 2 cut(s) 713, 2465
BmgT120I GGNCC 8 cut(s) 76, 440, 1430, 1566, 1718, 1719, 2355, 2560
BmiI GGNNCC 8 cut(s) 77, 117, 611, 741, 930, 1323, 1720, 1979
BmrFI CCNGG 6 cut(s) 26, 639, 1305, 1326, 1872, 2285
BmrI ACTGGG 4 cut(s) 687, 1500, 1976, 2035
BmtI GCTAGC 1 cut(s) 2493
BmuI ACTGGG 4 cut(s) 687, 1500, 1976, 2035
BpiI GAAGAC 2 cut(s) 1775, 2790
BplI GAGNNNNNCTC 2 cut(s) 1376, 1408
BpmI CTGGAG 1 cut(s) 2556
Bpu10I CCTNAGC 1 cut(s) 343
BpuEI CTTGAG 3 cut(s) 718, 2280, 2411
BpuMI CCSGG 1 cut(s) 26
Bsa29I ATCGAT 3 cut(s) 246, 891, 1445
BsaAI YACGTR 1 cut(s) 1397
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 201, 897, 1870, 2556
BsaWI WCCGGW 3 cut(s) 572, 1318, 2761
BsaXI ACNNNNNCTCC 4 cut(s) 724, 754, 2523, 2553
Bsc4I CCNNNNNNNGG 6 cut(s) 280, 449, 572, 956, 1325, 1517
Bse118I RCCGGY 2 cut(s) 449, 572
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 5 cut(s) 639, 1305, 1326, 1872, 2285
BseCI ATCGAT 3 cut(s) 246, 891, 1445
BseDI CCNNGG 4 cut(s) 201, 897, 1870, 2556
BseGI GGATG 5 cut(s) 463, 802, 1367, 1745, 2144
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 6 cut(s) 280, 449, 572, 956, 1325, 1517
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 5 cut(s) 357, 1182, 1853, 1923, 2163
BseRI GAGGAG 2 cut(s) 218, 851
BseSI GKGCMC 2 cut(s) 435, 1722
BseXI GCAGC 8 cut(s) 349, 371, 1630, 2174, 2184, 2206, 2389, 2469
BseYI CCCAGC 4 cut(s) 516, 1357, 1563, 2683
Bsh1236I CGCG 2 cut(s) 324, 2078
BshFI GGCC 4 cut(s) 1568, 1720, 1854, 2357
BshTI ACCGGT 1 cut(s) 572
BshVI ATCGAT 3 cut(s) 246, 891, 1445
BsiHKAI GWGCWC 1 cut(s) 1922
BsiHKCI CYCGRG 2 cut(s) 713, 2465
BsiSI CCGG 5 cut(s) 25, 450, 573, 1319, 2762
BslFI GGGAC 2 cut(s) 89, 2805
BslI CCNNNNNNNGG 6 cut(s) 280, 449, 572, 956, 1325, 1517
BsmAI GTCTC 2 cut(s) 1397, 2204
BsmFI GGGAC 2 cut(s) 89, 2805
BsnI GGCC 4 cut(s) 1568, 1720, 1854, 2357
BsoBI CYCGRG 2 cut(s) 713, 2465
Bsp120I GGGCCC 1 cut(s) 1718
Bsp1286I GDGCHC 5 cut(s) 435, 614, 742, 1722, 1922
Bsp143I GATC 7 cut(s) 11, 115, 239, 247, 706, 1747, 1977
BspACI CCGC 7 cut(s) 45, 54, 1686, 2078, 2138, 2498, 2501
BspANI GGCC 4 cut(s) 1568, 1720, 1854, 2357
BspCNI CTCAG 5 cut(s) 356, 1183, 1852, 1924, 2162
BspDI ATCGAT 3 cut(s) 246, 891, 1445
BspFNI CGCG 2 cut(s) 324, 2078
BspHI TCATGA 1 cut(s) 1414
BspLI GGNNCC 8 cut(s) 77, 117, 611, 741, 930, 1323, 1720, 1979
BspMAI CTGCAG 3 cut(s) 367, 1648, 1735
BspOI GCTAGC 1 cut(s) 2493
BspPI GGATC 6 cut(s) 110, 123, 247, 1755, 1972, 1985
BspTI CTTAAG 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 250
BsrFI RCCGGY 2 cut(s) 449, 572
BssAI RCCGGY 2 cut(s) 449, 572
BssECI CCNNGG 4 cut(s) 201, 897, 1870, 2556
BssMI GATC 7 cut(s) 11, 115, 239, 247, 706, 1747, 1977
BssNI GRCGYC 1 cut(s) 192
BssSI CACGAG 2 cut(s) 1434, 2326
BssT1I CCWWGG 3 cut(s) 201, 897, 2556
Bst2BI CACGAG 2 cut(s) 1434, 2326
Bst2UI CCWGG 5 cut(s) 639, 1305, 1326, 1872, 2285
Bst4CI ACNGT 6 cut(s) 508, 955, 1628, 1781, 2060, 2857
Bst6I CTCTTC 3 cut(s) 977, 1420, 1599
BstACI GRCGYC 1 cut(s) 192
BstAFI CTTAAG 1 cut(s) 551
BstBAI YACGTR 1 cut(s) 1397
BstC8I GCNNGC 5 cut(s) 326, 330, 2068, 2142, 2491
BstDEI CTNAG 9 cut(s) 209, 343, 454, 1191, 1839, 1932, 2149, 2312, 2628
BstEII GGTNACC 1 cut(s) 2416
BstF5I GGATG 5 cut(s) 463, 802, 1367, 1745, 2144
BstFNI CGCG 2 cut(s) 324, 2078
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 53, 565, 2518
BstKTI GATC 7 cut(s) 14, 118, 242, 250, 709, 1750, 1980
BstMAI GTCTC 2 cut(s) 1397, 2204
BstMBI GATC 7 cut(s) 11, 115, 239, 247, 706, 1747, 1977
BstMWI GCNNNNNNNGC 1 cut(s) 2495
BstNI CCWGG 5 cut(s) 639, 1305, 1326, 1872, 2285
BstPI GGTNACC 1 cut(s) 2416
BstSCI CCNGG 6 cut(s) 24, 637, 1303, 1324, 1870, 2283
BstSFI CTRYAG 4 cut(s) 363, 951, 1644, 1731
BstSLI GKGCMC 2 cut(s) 435, 1722
BstUI CGCG 2 cut(s) 324, 2078
BstV1I GCAGC 8 cut(s) 349, 371, 1630, 2174, 2184, 2206, 2389, 2469
BstV2I GAAGAC 2 cut(s) 1775, 2790
BstX2I RGATCY 3 cut(s) 115, 239, 1977
BstXI CCANNNNNNTGG 1 cut(s) 470
BstYI RGATCY 3 cut(s) 115, 239, 1977
Bsu15I ATCGAT 3 cut(s) 246, 891, 1445
BsuRI GGCC 4 cut(s) 1568, 1720, 1854, 2357
BsuTUI ATCGAT 3 cut(s) 246, 891, 1445
BtsCI GGATG 5 cut(s) 463, 802, 1367, 1745, 2144
BtsI GCAGTG 1 cut(s) 1728
BtsIMutI CAGTG 6 cut(s) 450, 675, 1728, 1906, 2365, 2411
Cac8I GCNNGC 5 cut(s) 326, 330, 2068, 2142, 2491
CciI TCATGA 1 cut(s) 1414
CfoI GCGC 3 cut(s) 53, 565, 2518
Cfr10I RCCGGY 2 cut(s) 449, 572
Cfr13I GGNCC 8 cut(s) 76, 440, 1430, 1566, 1718, 1719, 2355, 2560
ClaI ATCGAT 3 cut(s) 246, 891, 1445
CseI GACGC 2 cut(s) 181, 244
CsiI ACCWGGT 1 cut(s) 1303
Csp6I GTAC 6 cut(s) 504, 974, 1195, 1279, 1992, 2056
CspAI ACCGGT 1 cut(s) 572
CviAII CATG 6 cut(s) 1382, 1415, 1531, 1769, 1887, 1974
CviQI GTAC 6 cut(s) 504, 974, 1195, 1279, 1992, 2056
DdeI CTNAG 9 cut(s) 209, 343, 454, 1191, 1839, 1932, 2149, 2312, 2628
DpnI GATC 7 cut(s) 13, 117, 241, 249, 708, 1749, 1979
DpnII GATC 7 cut(s) 11, 115, 239, 247, 706, 1747, 1977
DraI TTTAAA 1 cut(s) 1255
Eam1104I CTCTTC 3 cut(s) 977, 1420, 1599
EarI CTCTTC 3 cut(s) 977, 1420, 1599
EciI GGCGGA 1 cut(s) 60
Ecl136II GAGCTC 1 cut(s) 1920
Eco130I CCWWGG 3 cut(s) 201, 897, 2556
Eco24I GRGCYC 4 cut(s) 614, 742, 1722, 1922
Eco47I GGWCC 4 cut(s) 76, 440, 1430, 2560
Eco53kI GAGCTC 1 cut(s) 1920
Eco88I CYCGRG 2 cut(s) 713, 2465
Eco91I GGTNACC 1 cut(s) 2416
EcoICRI GAGCTC 1 cut(s) 1920
EcoO65I GGTNACC 1 cut(s) 2416
EcoRII CCWGG 5 cut(s) 637, 1303, 1324, 1870, 2283
EcoT14I CCWWGG 3 cut(s) 201, 897, 2556
EcoT22I ATGCAT 3 cut(s) 880, 1150, 2309
EcoT38I GRGCYC 4 cut(s) 614, 742, 1722, 1922
ErhI CCWWGG 3 cut(s) 201, 897, 2556
FaeI CATG 6 cut(s) 1385, 1418, 1534, 1772, 1890, 1977
FalI AAGNNNNNCTT 4 cut(s) 774, 806, 1234, 1266
FaqI GGGAC 2 cut(s) 89, 2805
FatI CATG 6 cut(s) 1381, 1414, 1530, 1768, 1886, 1973
FauI CCCGC 1 cut(s) 2145
FauNDI CATATG 2 cut(s) 1150, 1924
FbaI TGATCA 1 cut(s) 706
FblI GTMKAC 1 cut(s) 2250
FokI GGATG 5 cut(s) 450, 809, 1374, 1752, 2131
FriOI GRGCYC 4 cut(s) 614, 742, 1722, 1922
FspBI CTAG 9 cut(s) 303, 603, 1095, 1754, 2123, 2171, 2384, 2490, 2589
GlaI GCGC 3 cut(s) 52, 564, 2517
GsaI CCCAGC 4 cut(s) 520, 1361, 1567, 2687
GsuI CTGGAG 1 cut(s) 2556
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 4 cut(s) 1568, 1720, 1854, 2357
HapII CCGG 5 cut(s) 25, 450, 573, 1319, 2762
HgaI GACGC 2 cut(s) 181, 244
HhaI GCGC 3 cut(s) 53, 565, 2518
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 6 cut(s) 1385, 1418, 1534, 1772, 1890, 1977
Hin6I GCGC 3 cut(s) 51, 563, 2516
HinP1I GCGC 3 cut(s) 51, 563, 2516
HincII GTYRAC 1 cut(s) 2776
HindII GTYRAC 1 cut(s) 2776
HindIII AAGCTT 3 cut(s) 686, 2274, 2429
HpaI GTTAAC 1 cut(s) 2776
HpaII CCGG 5 cut(s) 25, 450, 573, 1319, 2762
HphI GGTGA 9 cut(s) 173, 187, 485, 716, 1613, 1621, 1813, 2107, 2776
Hpy99I CGWCG 2 cut(s) 169, 2829
HpyAV CCTTC 7 cut(s) 67, 371, 1191, 1592, 1797, 1901, 2666
HpyCH4III ACNGT 6 cut(s) 508, 955, 1628, 1781, 2060, 2857
HpyCH4IV ACGT 4 cut(s) 734, 1396, 1879, 2827
HpyF10VI GCNNNNNNNGC 1 cut(s) 2495
HpyF3I CTNAG 9 cut(s) 209, 343, 454, 1191, 1839, 1932, 2149, 2312, 2628
HpySE526I ACGT 4 cut(s) 734, 1396, 1879, 2827
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 6 cut(s) 1385, 1418, 1534, 1772, 1890, 1977
HspAI GCGC 3 cut(s) 51, 563, 2516
Ksp22I TGATCA 1 cut(s) 706
KspAI GTTAAC 1 cut(s) 2776
Kzo9I GATC 7 cut(s) 11, 115, 239, 247, 706, 1747, 1977
LmnI GCTCC 6 cut(s) 27, 609, 745, 2159, 2371, 2644
Lsp1109I GCAGC 8 cut(s) 349, 371, 1630, 2174, 2184, 2206, 2389, 2469
MabI ACCWGGT 1 cut(s) 1303
MaeI CTAG 9 cut(s) 303, 603, 1095, 1754, 2123, 2171, 2384, 2490, 2589
MaeII ACGT 4 cut(s) 734, 1396, 1879, 2827
MalI GATC 7 cut(s) 13, 117, 241, 249, 708, 1749, 1979
MboI GATC 7 cut(s) 11, 115, 239, 247, 706, 1747, 1977
MflI RGATCY 3 cut(s) 115, 239, 1977
MhlI GDGCHC 5 cut(s) 435, 614, 742, 1722, 1922
MluCI AATT 8 cut(s) 284, 830, 1026, 1660, 1808, 2450, 2744, 2749
MlyI GAGTC 7 cut(s) 135, 212, 358, 543, 1824, 1831, 2206
Mph1103I ATGCAT 3 cut(s) 880, 1150, 2309
MslI CAYNNNNRTG 2 cut(s) 468, 1342
MspA1I CMGCKG 2 cut(s) 362, 2197
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 5 cut(s) 25, 450, 573, 1319, 2762
MspR9I CCNGG 6 cut(s) 26, 639, 1305, 1326, 1872, 2285
MvaI CCWGG 5 cut(s) 639, 1305, 1326, 1872, 2285
MvnI CGCG 2 cut(s) 324, 2078
MwoI GCNNNNNNNGC 1 cut(s) 2495
NciI CCSGG 1 cut(s) 26
NdeI CATATG 2 cut(s) 1150, 1924
NdeII GATC 7 cut(s) 11, 115, 239, 247, 706, 1747, 1977
NheI GCTAGC 1 cut(s) 2489
NlaIII CATG 6 cut(s) 1385, 1418, 1534, 1772, 1890, 1977
NlaIV GGNNCC 8 cut(s) 77, 117, 611, 741, 930, 1323, 1720, 1979
NmuCI GTSAC 7 cut(s) 97, 193, 445, 1206, 1726, 2095, 2714
NsiI ATGCAT 3 cut(s) 880, 1150, 2309
PaeR7I CTCGAG 2 cut(s) 713, 2465
PagI TCATGA 1 cut(s) 1414
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 863
PflFI GACNNNGTC 1 cut(s) 191
PfoI TCCNGGA 1 cut(s) 24
PinAI ACCGGT 1 cut(s) 572
PleI GAGTC 7 cut(s) 135, 212, 357, 542, 1823, 1831, 2206
PpsI GAGTC 7 cut(s) 135, 212, 357, 542, 1823, 1831, 2206
Ppu21I YACGTR 1 cut(s) 1397
Psp124BI GAGCTC 1 cut(s) 1922
Psp6I CCWGG 5 cut(s) 637, 1303, 1324, 1870, 2283
PspEI GGTNACC 1 cut(s) 2416
PspFI CCCAGC 4 cut(s) 516, 1357, 1563, 2683
PspGI CCWGG 5 cut(s) 637, 1303, 1324, 1870, 2283
PspN4I GGNNCC 8 cut(s) 77, 117, 611, 741, 930, 1323, 1720, 1979
PspOMI GGGCCC 1 cut(s) 1718
PspPI GGNCC 8 cut(s) 76, 440, 1430, 1566, 1718, 1719, 2355, 2560
PspXI VCTCGAGB 1 cut(s) 2465
PstI CTGCAG 3 cut(s) 367, 1648, 1735
PsuI RGATCY 3 cut(s) 115, 239, 1977
PsyI GACNNNGTC 1 cut(s) 191
PvuII CAGCTG 2 cut(s) 362, 2197
RsaI GTAC 6 cut(s) 505, 975, 1196, 1280, 1993, 2057
RsaNI GTAC 6 cut(s) 504, 974, 1195, 1279, 1992, 2056
RseI CAYNNNNRTG 2 cut(s) 468, 1342
SacI GAGCTC 1 cut(s) 1922
Sau3AI GATC 7 cut(s) 11, 115, 239, 247, 706, 1747, 1977
Sau96I GGNCC 8 cut(s) 76, 440, 1430, 1566, 1718, 1719, 2355, 2560
SchI GAGTC 7 cut(s) 135, 212, 358, 543, 1824, 1831, 2206
ScrFI CCNGG 6 cut(s) 26, 639, 1305, 1326, 1872, 2285
SduI GDGCHC 5 cut(s) 435, 614, 742, 1722, 1922
SexAI ACCWGGT 1 cut(s) 1303
SfcI CTRYAG 4 cut(s) 363, 951, 1644, 1731
Sfr274I CTCGAG 2 cut(s) 713, 2465
SinI GGWCC 4 cut(s) 76, 440, 1430, 2560
SlaI CTCGAG 2 cut(s) 713, 2465
SmiMI CAYNNNNRTG 2 cut(s) 468, 1342
SmlI CTYRAG 6 cut(s) 551, 697, 713, 2295, 2426, 2465
SmoI CTYRAG 6 cut(s) 551, 697, 713, 2295, 2426, 2465
SpeI ACTAGT 2 cut(s) 1094, 2588
Sse9I AATT 8 cut(s) 284, 830, 1026, 1660, 1808, 2450, 2744, 2749
SsiI CCGC 7 cut(s) 45, 54, 1686, 2078, 2138, 2498, 2501
SspI AATATT 1 cut(s) 598
SspMI CTAG 9 cut(s) 303, 603, 1095, 1754, 2123, 2171, 2384, 2490, 2589
SstI GAGCTC 1 cut(s) 1922
StyD4I CCNGG 6 cut(s) 24, 637, 1303, 1324, 1870, 2283
StyI CCWWGG 3 cut(s) 201, 897, 2556
TaaI ACNGT 6 cut(s) 508, 955, 1628, 1781, 2060, 2857
TaiI ACGT 4 cut(s) 737, 1399, 1882, 2830
TasI AATT 8 cut(s) 284, 830, 1026, 1660, 1808, 2450, 2744, 2749
TatI WGTACW 3 cut(s) 1194, 1278, 2055
TauI GCSGC 2 cut(s) 56, 2501
TscAI CASTG 6 cut(s) 450, 682, 1735, 1906, 2365, 2418
TseFI GTSAC 7 cut(s) 97, 193, 445, 1206, 1726, 2095, 2714
TseI GCWGC 8 cut(s) 359, 362, 1643, 2162, 2194, 2197, 2402, 2457
Tsp45I GTSAC 7 cut(s) 97, 193, 445, 1206, 1726, 2095, 2714
TspGWI ACGGA 5 cut(s) 118, 164, 546, 1348, 2544
TspRI CASTG 6 cut(s) 450, 682, 1735, 1906, 2365, 2418
Tth111I GACNNNGTC 1 cut(s) 191
Vha464I CTTAAG 1 cut(s) 551
VpaK11BI GGWCC 4 cut(s) 76, 440, 1430, 2560
XapI RAATTY 6 cut(s) 284, 830, 1026, 1808, 2450, 2749
XbaI TCTAGA 2 cut(s) 2170, 2383
XcmI CCANNNNNNNNNTGG 2 cut(s) 1560, 2365
XhoI CTCGAG 2 cut(s) 713, 2465
XmiI GTMKAC 1 cut(s) 2250
XspI CTAG 9 cut(s) 303, 603, 1095, 1754, 2123, 2171, 2384, 2490, 2589
Zsp2I ATGCAT 3 cut(s) 880, 1150, 2309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.