FvH4_5g13280

WD repeat and HMG-box DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005318
Physical Location & Seq
Reverse (-)
7500225 .. 7505918
5694 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13280.t4

Sequence Viewer

Length: 2871 bp
ATGAAGGTCAGGCAAGTGAAGCTCCGCGAGATCCACAAATCGGCGAGCAGCGCAGGAAAGCCGTCGTTCTGCTCCGTCGTGTGGGACCAGCAAGGCTTCCACTCCGTGACGGCTTCCTCCTCCGACGCCTCAATCGCCGTCCACAACGCTCTTATCTCCTCATGTCCGCCGAAGCTCCTCCGCAACCACCGCGACGGCGTCACCGCGCTCGCTATGAGCCCCAACGCCACCTGCCTCGCCTCCGGCTCCGTCGACCACTCCGTCAAGCTCTACAAGTTTCCTAGTGGAGACTTTGAGACTAATATCACCAGATTCACGCTGCCAATACGTGCCCTTGCGTTTAATAAGTCAGGGACACTCTTGGCCGCTGCTGGTGATGATGAGGGAATTAAGCTTATTAACACAGTAGATGGTACAATTTCGAAGGTTCTCAAAGCACACAAAGGACCTGTAACTGGTTTAGGTTTTGATCCCCAAAGTGAATACATGGCCTCCATTGATTCAACTGGGACTGTAATATACTGGGAACTTCAATCTGGAAGCATATTGCACACCCTTAAAGCCGTAGCTCCTAACTCTGGTTTAGACCTTTCTGTATTGAATGTATTGAGCTGGAGTCCTGATGGACGGTTGTTAGCTGTCCCGGGAGTGCGAAATGATGTTGTGTTGTATGATAAAGACACTGCTGAGAAGCTCTTTACCTTGAGAGGTGAACATACACAACCTATTTGTGCCTTGGCCTGGTCGCCTAATGGAAAATATTTGGCTACATCTAGTTTGGATAAGCAGGTTCTCATCTGGGATGTTGATCGAAAGCAAGATATTGGCAGGCATAAATTTGATGAGAGGATAACTAGCTTGGCTTGGAAGCCAATTGACAACGTCCTAGCTGTAATTGATTGTATGGGCAGGTATGGTGTGTGGGAAGCAGTGATTCCCTCGTCAATGAAATCTCCTACTGAAGATGTTCCGAATCAATCTAATAATGGTCTTCTCTTGTTTGATGAAGACGAAGTCGAGGGTCAGGAGAGTAGTACATCTGGTAGTTTAAGTGATCTTGGTGAAGATACATATGAGGAGTTGAAGCCACTTAGCAGGAAAAGACTAAGCAAGCAGTCTGACGCTCTGAAAGAGTTAGATGAGGATGATAATGATGACTTTAGTTTGTCCCCTAAAGTTGAATCCCATAAAAAAGCACGCCGGCATCATAATAAAAGCACTGATAATGGGAATGAGGGAATAAGAAGCAGATTGCCATCTGCTAGGTCTAAAATGCAGGAGGCATTTCAGCCAGGGGCTACTCCTGTGCAGCCTGGCAAAAGGCGCTTTTTGTGCTACAACATGCTTGGAGCTATAACTACGATTGAATACGAAGGGTACTCCCATATTGAGATAGATTTTCATGACACAAGCCGAGGTCCACGAGTTCCGTCAATGACCGATCATTTTGGCTTCACAATGGCTTCGCTGAATGAGAATGGTAGTGTTTTTGCAAATCCCTCCAAGGGTGAAAAGAACATGAGCACTCTCATGTATCGTCCATTTACTAGCTGGGCAAACAATAGCGAGTGGTCTATGCGATTGGAAGGGGAAGAAGTGAAGGTTGTGGCCCTTGGTACAACTTGGGTGGCAGCAGTTACCAGTCTAAACTTCATCCGCATCTTCACCGTTGGTGGTTTGCAGACACATGTTCTTTCCCTTGATGGACCAGTGGTGACTGCATCAGGCTTCAAGGATGAGCTTGCCATTGTCACTCATGCTTCAGACTGTCTTCCCTCAAATGAGCAGGTGCTTGAATTTAGGGTACTTAATATGACTAATAGTAGCCAACCTCTTAGAGGTCGTCTGCCTCTGACTCCTGGTTCAAGTTTAACATGGTTTGGCTTCAGTGAAGAAGGGAAATTAAGCTCCTGTGACTCCAAGGGTGTGCTGAGAGTTTTTACAAGTGAATATGGTGGCAGTTGGTTCCCACTCTTCAGTGCTTGTAAAGACAAGAAGCCGGATGAAAACTATTGGGTGGTAGGTTTGAATGCAAGACAACTATTTTGTATAGTCTGCAAAAGCCCTGACTTGTATCCCCAGGTGGTGCCCAAACCAGTTCTCACTCTACTGAATTTTTCGATTCCTCTCGCATCCTCTGATCTAGGAGCTGAAGCCCTTGAAAATGCGTTTATACTAAACAACATGCATCTCATAGAGATTCAAAAGCAAATAGAAGAAATGGATGCTGCTGGTTTGGACACCAGTTCACTCGATGATGAAGCTTTCAAATTGGAAACAGGTCAAGATCAATGTATATTGAGGCTTATTGGATCCTGCTGCCATGGAGATAAGCTGGAGAGGGCTACTGAACTTGTGAAGCATCTATCCCTGGAAAAGTCAGTCAGGGGTGCAATTAAGATTGTTACTTCCCTGAAGCTACCTAATTTGGCTGAAAGGTTCAATAGCATATTGGAGGAAAGGTTGCTCACCGAAAATAAACCATTGCAAACAACTGTCCCTAACTCAAACTACAATGCATCATCCTCTACCGAGAAAAGTAGGTTATCAGGGAATGATATTCCTTTACCAGCACCAAAATTGTCGGCTCCCATGTTTCTAAAGAAAGCCAGAACACAAGAAGCTATTGTTGGAACGGAGAAAACACAAGAAAAGCAAACTCCCGCAGTGAATGATCTTGAGCAGTCGAAGACGACTGAGAGGGAAAGTGCTGCTGCAGAGGTTAATAATGCAGCAGGTGACATGAACAAGGACCGCCCATCTAAACCATTTCTAAAGCGAACAAACAGTCAGGACATAGAGAAGAAGGATGAAGTAGACAAGGTTAAAGCTCACCGACCAGCTAATCCCTTCAAGAAATCAGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

957

Amino Acids

105.08

Weight (kDa)

6.15

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 9 - 147 3.8e-15 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 22 - 142 1.8e-10 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 25 - 307 1.9e-69 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 25 - 187 1.8e-23 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 38 - 185 2.9e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 59 - 188 2.6e-18 WDR3 first beta-propeller domain
WD40 PF00400 59 - 91 5.4e-06 WD domain, G-beta repeat
Beta-prop_EML PF23409 62 - 282 9.3e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 199 1.8e-10 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 64 - 298 1.2e-32 Prp19 WD40 domain
WDR55 PF24796 64 - 188 7.2e-10 WDR55
Beta-prop_WDR3_2nd PF25172 64 - 187 2.2e-10 WDR3 second beta-propeller domain
Beta-prop_WDR19_1st PF23389 116 - 270 1.2e-13 WDR19 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 144 - 272 3.6e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 147 - 298 6.8e-19 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 172 - 296 2.5e-11 WDR3 first beta-propeller domain
Beta-prop_IFT122_1st PF23381 200 - 310 7.7e-06 IFT122 first beta-propeller
Beta-prop_EML_2 PF23414 201 - 313 1.7e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT140_1st PF23383 201 - 282 8.5e-07 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 202 - 297 1.3e-11 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 203 - 290 1.2e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_CDC20-Fz PF24807 203 - 296 2.4e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 205 - 299 6.4e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 228 - 297 2.8e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 228 - 309 3.8e-07 Aladin seven-bladed propeller
WD40 PF00400 232 - 268 1.2e-08 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 232 - 274 5.8e-06 TEP-1 second beta-propeller
Mcl1_mid PF12341 427 - 696 5.6e-88 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 746 - 847 3.5e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 239, 1777, 2726
AasI GACNNNNNNGTC 1 cut(s) 260
Acc36I ACCTGC 5 cut(s) 239, 778, 900, 1777, 2726
AccB1I GGYRCC 1 cut(s) 2086
AccI GTMKAC 2 cut(s) 252, 2817
AccII CGCG 3 cut(s) 27, 192, 206
AciI CCGC 9 cut(s) 25, 167, 181, 190, 204, 366, 1657, 2664, 2755
AclWI GGATC 4 cut(s) 25, 464, 2307, 2320
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 3 cut(s) 836, 1796, 2113
AcuI CTGAAG 6 cut(s) 981, 1746, 1870, 1960, 2172, 2435
AcyI GRCGYC 2 cut(s) 126, 198
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 5 cut(s) 415, 1036, 1379, 1618, 1806
AfiI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
AflIII ACRYGT 1 cut(s) 1687
AjnI CCWGG 6 cut(s) 740, 1291, 1312, 1858, 2079, 2370
AjuI GAANNNNNNNTTGG 2 cut(s) 2613, 2645
Alw21I GWGCWC 1 cut(s) 1526
Alw26I GTCTC 2 cut(s) 282, 290
AlwI GGATC 4 cut(s) 25, 464, 2307, 2320
Ama87I CYCGRG 1 cut(s) 643
AoxI GGCC 4 cut(s) 363, 489, 738, 1608
ApoI RAATTY 3 cut(s) 836, 1796, 2113
ArsI GACNNNNNNTTYG 4 cut(s) 2028, 2060, 2773, 2805
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 3 cut(s) 53, 208, 1326
AspS9I GGNCC 6 cut(s) 85, 446, 1418, 1609, 1706, 2752
AsuC2I CCSGG 2 cut(s) 644, 645
AsuII TTCGAA 1 cut(s) 422
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 5 cut(s) 85, 446, 1418, 1706, 2752
BaeGI GKGCMC 2 cut(s) 334, 2091
BamHI GGATCC 1 cut(s) 2312
BanI GGYRCC 1 cut(s) 2086
BanII GRGCYC 1 cut(s) 221
BauI CACGAG 1 cut(s) 1422
BbsI GAAGAC 4 cut(s) 983, 1014, 1763, 2696
Bbv12I GWGCWC 1 cut(s) 1526
BccI CCATC 5 cut(s) 404, 617, 1264, 1697, 2767
BceAI ACGGC 5 cut(s) 46, 122, 126, 211, 548
BciT130I CCWGG 6 cut(s) 742, 1293, 1314, 1860, 2081, 2372
BciVI GTATCC 1 cut(s) 2085
BcnI CCSGG 2 cut(s) 644, 645
BcoDI GTCTC 2 cut(s) 282, 290
BfaI CTAG 7 cut(s) 282, 774, 855, 887, 1263, 1548, 2144
BfmI CTRYAG 1 cut(s) 2715
BfoI RGCGCY 1 cut(s) 1327
BfuAI ACCTGC 5 cut(s) 239, 778, 900, 1777, 2726
BfuI GTATCC 1 cut(s) 2085
Bme1390I CCNGG 8 cut(s) 644, 645, 742, 1293, 1314, 1860, 2081, 2372
Bme18I GGWCC 5 cut(s) 85, 446, 1418, 1706, 2752
BmeT110I CYCGRG 1 cut(s) 643
BmgT120I GGNCC 6 cut(s) 85, 446, 1418, 1609, 1706, 2752
BmiI GGNNCC 6 cut(s) 86, 247, 1967, 2088, 2314, 2589
BmrFI CCNGG 8 cut(s) 644, 645, 742, 1293, 1314, 1860, 2081, 2372
BmrI ACTGGG 2 cut(s) 516, 532
BmsI GCATC 8 cut(s) 1213, 1668, 1730, 2141, 2197, 2215, 2371, 2528
BmuI ACTGGG 2 cut(s) 516, 532
BpiI GAAGAC 4 cut(s) 983, 1014, 1763, 2696
BpmI CTGGAG 2 cut(s) 634, 2357
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 2 cut(s) 724, 2699
BpuMI CCSGG 2 cut(s) 644, 645
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 2 cut(s) 807, 1255
BsaHI GRCGYC 2 cut(s) 126, 198
BsaXI ACNNNNNCTCC 4 cut(s) 476, 506, 2447, 2477
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
Bse118I RCCGGY 1 cut(s) 1200
Bse1I ACTGG 7 cut(s) 460, 511, 527, 1641, 1709, 2096, 2244
Bse3DI GCAATG 1 cut(s) 2483
Bse8I GATNNNNATC 2 cut(s) 807, 1255
BseBI CCWGG 6 cut(s) 742, 1293, 1314, 1860, 2081, 2372
BseGI GGATG 9 cut(s) 808, 1150, 1653, 1741, 2008, 2132, 2230, 2522, 2815
BseJI GATNNNNATC 2 cut(s) 807, 1255
BseLI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
BseMI GCAATG 1 cut(s) 2483
BseMII CTCAG 3 cut(s) 678, 1922, 2688
BseNI ACTGG 7 cut(s) 460, 511, 527, 1641, 1709, 2096, 2244
BseRI GAGGAG 4 cut(s) 109, 148, 167, 1091
BseSI GKGCMC 2 cut(s) 334, 2091
BseYI CCCAGC 1 cut(s) 1551
BsgI GTGCAG 1 cut(s) 1328
Bsh1236I CGCG 3 cut(s) 27, 192, 206
BshFI GGCC 4 cut(s) 365, 491, 740, 1610
BshNI GGYRCC 1 cut(s) 2086
BsiHKAI GWGCWC 1 cut(s) 1526
BsiHKCI CYCGRG 1 cut(s) 643
BsiSI CCGG 4 cut(s) 243, 644, 1201, 2000
BslFI GGGAC 6 cut(s) 98, 367, 523, 626, 1153, 2483
BslI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
BsmAI GTCTC 2 cut(s) 282, 290
BsmFI GGGAC 6 cut(s) 98, 367, 523, 626, 1153, 2483
BsmI GAATGC 1 cut(s) 2035
BsnI GGCC 4 cut(s) 365, 491, 740, 1610
BsoBI CYCGRG 1 cut(s) 643
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 4 cut(s) 221, 334, 1526, 2091
Bsp143I GATC 9 cut(s) 30, 469, 808, 1054, 1441, 2140, 2287, 2312, 2674
Bsp19I CCATGG 1 cut(s) 2323
BspACI CCGC 9 cut(s) 25, 167, 181, 190, 204, 366, 1657, 2664, 2755
BspANI GGCC 4 cut(s) 365, 491, 740, 1610
BspCNI CTCAG 3 cut(s) 679, 1923, 2689
BspFNI CGCG 3 cut(s) 27, 192, 206
BspHI TCATGA 1 cut(s) 1402
BspLI GGNNCC 6 cut(s) 86, 247, 1967, 2088, 2314, 2589
BspMAI CTGCAG 1 cut(s) 2719
BspMI ACCTGC 5 cut(s) 239, 778, 900, 1777, 2726
BspPI GGATC 4 cut(s) 25, 464, 2307, 2320
BspT104I TTCGAA 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 2086
BsrDI GCAATG 1 cut(s) 2483
BsrFI RCCGGY 1 cut(s) 1200
BsrI ACTGG 7 cut(s) 460, 511, 527, 1641, 1709, 2096, 2244
BssAI RCCGGY 1 cut(s) 1200
BssMI GATC 9 cut(s) 30, 469, 808, 1054, 1441, 2140, 2287, 2312, 2674
BssNI GRCGYC 2 cut(s) 126, 198
BssSI CACGAG 1 cut(s) 1422
BssT1I CCWWGG 5 cut(s) 735, 1503, 1612, 1920, 2323
Bst2BI CACGAG 1 cut(s) 1422
Bst2UI CCWGG 6 cut(s) 742, 1293, 1314, 1860, 2081, 2372
Bst4CI ACNGT 7 cut(s) 406, 514, 630, 1669, 1769, 2497, 2789
Bst6I CTCTTC 1 cut(s) 1979
BstACI GRCGYC 2 cut(s) 126, 198
BstBAI YACGTR 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 7 cut(s) 46, 210, 830, 1112, 1198, 1202, 1743
BstDEI CTNAG 6 cut(s) 687, 1091, 1106, 1835, 1931, 2697
BstDSI CCRYGG 1 cut(s) 2323
BstENI CCTNNNNNAGG 1 cut(s) 1836
BstF5I GGATG 9 cut(s) 808, 1150, 1653, 1741, 2008, 2132, 2230, 2522, 2815
BstFNI CGCG 3 cut(s) 27, 192, 206
BstH2I RGCGCY 1 cut(s) 1327
BstHHI GCGC 3 cut(s) 53, 208, 1326
BstKTI GATC 9 cut(s) 33, 472, 811, 1057, 1444, 2143, 2290, 2315, 2677
BstMAI GTCTC 2 cut(s) 282, 290
BstMBI GATC 9 cut(s) 30, 469, 808, 1054, 1441, 2140, 2287, 2312, 2674
BstMWI GCNNNNNNNGC 6 cut(s) 19, 50, 134, 189, 1323, 1332
BstNI CCWGG 6 cut(s) 742, 1293, 1314, 1860, 2081, 2372
BstNSI RCATGY 3 cut(s) 1345, 1691, 2188
BstSCI CCNGG 8 cut(s) 642, 643, 740, 1291, 1312, 1858, 2079, 2370
BstSFI CTRYAG 1 cut(s) 2715
BstSLI GKGCMC 2 cut(s) 334, 2091
BstUI CGCG 3 cut(s) 27, 192, 206
BstV2I GAAGAC 4 cut(s) 983, 1014, 1763, 2696
BstX2I RGATCY 2 cut(s) 30, 2312
BstYI RGATCY 2 cut(s) 30, 2312
BsuI GTATCC 1 cut(s) 2085
BsuRI GGCC 4 cut(s) 365, 491, 740, 1610
BtgI CCRYGG 1 cut(s) 2323
BtsCI GGATG 9 cut(s) 808, 1150, 1653, 1741, 2008, 2132, 2230, 2522, 2815
BtsI GCAGTG 3 cut(s) 681, 936, 2673
BtsIMutI CAGTG 7 cut(s) 681, 936, 1218, 1716, 1894, 1984, 2673
BveI ACCTGC 5 cut(s) 239, 778, 900, 1777, 2726
Cac8I GCNNGC 7 cut(s) 46, 210, 830, 1112, 1198, 1202, 1743
CciI TCATGA 1 cut(s) 1402
CfoI GCGC 3 cut(s) 53, 208, 1326
Cfr10I RCCGGY 1 cut(s) 1200
Cfr13I GGNCC 6 cut(s) 85, 446, 1418, 1609, 1706, 2752
Cfr9I CCCGGG 1 cut(s) 643
CseI GACGC 3 cut(s) 134, 187, 1130
Csp6I GTAC 5 cut(s) 414, 1035, 1378, 1617, 1805
CspCI CAANNNNNGTGG 2 cut(s) 1608, 1643
CviQI GTAC 5 cut(s) 414, 1035, 1378, 1617, 1805
DdeI CTNAG 6 cut(s) 687, 1091, 1106, 1835, 1931, 2697
DpnI GATC 9 cut(s) 32, 471, 810, 1056, 1443, 2142, 2289, 2314, 2676
DpnII GATC 9 cut(s) 30, 469, 808, 1054, 1441, 2140, 2287, 2312, 2674
DraIII CACNNNGTG 1 cut(s) 106
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 1979
EarI CTCTTC 1 cut(s) 1979
EciI GGCGGA 1 cut(s) 156
Eco130I CCWWGG 5 cut(s) 735, 1503, 1612, 1920, 2323
Eco24I GRGCYC 1 cut(s) 221
Eco47I GGWCC 5 cut(s) 85, 446, 1418, 1706, 2752
Eco57I CTGAAG 6 cut(s) 981, 1746, 1870, 1960, 2172, 2435
Eco88I CYCGRG 1 cut(s) 643
EcoNI CCTNNNNNAGG 1 cut(s) 1836
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 6 cut(s) 740, 1291, 1312, 1858, 2079, 2370
EcoT14I CCWWGG 5 cut(s) 735, 1503, 1612, 1920, 2323
EcoT22I ATGCAT 2 cut(s) 2190, 2521
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 5 cut(s) 735, 1503, 1612, 1920, 2323
FaqI GGGAC 6 cut(s) 98, 367, 523, 626, 1153, 2483
FauI CCCGC 1 cut(s) 2671
FauNDI CATATG 1 cut(s) 1072
FblI GTMKAC 2 cut(s) 252, 2817
FokI GGATG 9 cut(s) 815, 1157, 1640, 1748, 2015, 2119, 2237, 2509, 2822
FriOI GRGCYC 1 cut(s) 221
FspBI CTAG 7 cut(s) 282, 774, 855, 887, 1263, 1548, 2144
GlaI GCGC 3 cut(s) 52, 207, 1325
GsaI CCCAGC 1 cut(s) 1555
GsuI CTGGAG 2 cut(s) 634, 2357
HaeII RGCGCY 1 cut(s) 1327
HaeIII GGCC 4 cut(s) 365, 491, 740, 1610
HapII CCGG 4 cut(s) 243, 644, 1201, 2000
HgaI GACGC 3 cut(s) 134, 187, 1130
HhaI GCGC 3 cut(s) 53, 208, 1326
Hin1I GRCGYC 2 cut(s) 126, 198
Hin6I GCGC 3 cut(s) 51, 206, 1324
HinP1I GCGC 3 cut(s) 51, 206, 1324
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 2 cut(s) 392, 2262
HpaII CCGG 4 cut(s) 243, 644, 1201, 2000
Hpy166II GTNNAC 6 cut(s) 142, 253, 713, 1421, 2249, 2818
Hpy188I TCNGA 7 cut(s) 124, 972, 1120, 1128, 1765, 1854, 2140
Hpy188III TCNNGA 8 cut(s) 537, 620, 1025, 1403, 2285, 2678, 2792, 2854
Hpy8I GTNNAC 6 cut(s) 142, 253, 713, 1421, 2249, 2818
Hpy99I CGWCG 5 cut(s) 67, 80, 128, 197, 254
HpyAV CCTTC 7 cut(s) 418, 1367, 1580, 1594, 1889, 2800, 2860
HpyCH4III ACNGT 7 cut(s) 406, 514, 630, 1669, 1769, 2497, 2789
HpyCH4IV ACGT 2 cut(s) 328, 882
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 50, 134, 189, 1323, 1332
HpyF3I CTNAG 6 cut(s) 687, 1091, 1106, 1835, 1931, 2697
HpySE526I ACGT 2 cut(s) 328, 882
Hsp92I GRCGYC 2 cut(s) 126, 198
HspAI GCGC 3 cut(s) 51, 206, 1324
KroI GCCGGC 1 cut(s) 1200
KroNI GCCGGC 1 cut(s) 1202
Kzo9I GATC 9 cut(s) 30, 469, 808, 1054, 1441, 2140, 2287, 2312, 2674
LmnI GCTCC 9 cut(s) 27, 77, 180, 251, 574, 1349, 1913, 2147, 2593
LweI GCATC 8 cut(s) 1213, 1668, 1730, 2141, 2197, 2215, 2371, 2528
MaeI CTAG 7 cut(s) 282, 774, 855, 887, 1263, 1548, 2144
MaeII ACGT 2 cut(s) 328, 882
MaeIII GTNAC 9 cut(s) 106, 199, 451, 1636, 1714, 1750, 1913, 2404, 2738
MalI GATC 9 cut(s) 32, 471, 810, 1056, 1443, 2142, 2289, 2314, 2676
MboI GATC 9 cut(s) 30, 469, 808, 1054, 1441, 2140, 2287, 2312, 2674
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 2 cut(s) 30, 2312
MhlI GDGCHC 4 cut(s) 221, 334, 1526, 2091
MlyI GAGTC 3 cut(s) 625, 1849, 1910
MmeI TCCRAC 2 cut(s) 147, 2611
Mph1103I ATGCAT 2 cut(s) 2190, 2521
MroNI GCCGGC 1 cut(s) 1200
MroXI GAANNNNTTC 1 cut(s) 966
MslI CAYNNNNRTG 1 cut(s) 1529
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 4 cut(s) 243, 644, 1201, 2000
MspR9I CCNGG 8 cut(s) 644, 645, 742, 1293, 1314, 1860, 2081, 2372
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 2035
MvaI CCWGG 6 cut(s) 742, 1293, 1314, 1860, 2081, 2372
MvnI CGCG 3 cut(s) 27, 192, 206
MwoI GCNNNNNNNGC 6 cut(s) 19, 50, 134, 189, 1323, 1332
NaeI GCCGGC 1 cut(s) 1202
NciI CCSGG 2 cut(s) 644, 645
NcoI CCATGG 1 cut(s) 2323
NdeI CATATG 1 cut(s) 1072
NdeII GATC 9 cut(s) 30, 469, 808, 1054, 1441, 2140, 2287, 2312, 2674
NgoMIV GCCGGC 1 cut(s) 1200
NlaIV GGNNCC 6 cut(s) 86, 247, 1967, 2088, 2314, 2589
NmeAIII GCCGAG 1 cut(s) 1439
NmuCI GTSAC 6 cut(s) 106, 199, 1714, 1750, 1913, 2738
NsiI ATGCAT 2 cut(s) 2190, 2521
NspI RCATGY 3 cut(s) 1345, 1691, 2188
NspV TTCGAA 1 cut(s) 422
PagI TCATGA 1 cut(s) 1402
PaqCI CACCTGC 3 cut(s) 239, 1777, 2726
PciI ACATGT 1 cut(s) 1687
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 1 cut(s) 2035
PdiI GCCGGC 1 cut(s) 1202
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 7 cut(s) 312, 500, 934, 973, 1181, 2122, 2200
PflFI GACNNNGTC 3 cut(s) 197, 881, 1013
PleI GAGTC 3 cut(s) 624, 1849, 1910
PpsI GAGTC 3 cut(s) 624, 1849, 1910
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 1 cut(s) 1687
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 6 cut(s) 740, 1291, 1312, 1858, 2079, 2370
PspFI CCCAGC 1 cut(s) 1551
PspGI CCWGG 6 cut(s) 740, 1291, 1312, 1858, 2079, 2370
PspN4I GGNNCC 6 cut(s) 86, 247, 1967, 2088, 2314, 2589
PspPI GGNCC 6 cut(s) 85, 446, 1418, 1609, 1706, 2752
PspPPI RGGWCCY 1 cut(s) 446
PstI CTGCAG 1 cut(s) 2719
PsuI RGATCY 2 cut(s) 30, 2312
PsyI GACNNNGTC 3 cut(s) 197, 881, 1013
RsaI GTAC 5 cut(s) 415, 1036, 1379, 1618, 1806
RsaNI GTAC 5 cut(s) 414, 1035, 1378, 1617, 1805
RseI CAYNNNNRTG 1 cut(s) 1529
SalI GTCGAC 1 cut(s) 251
Sau3AI GATC 9 cut(s) 30, 469, 808, 1054, 1441, 2140, 2287, 2312, 2674
Sau96I GGNCC 6 cut(s) 85, 446, 1418, 1609, 1706, 2752
SchI GAGTC 3 cut(s) 625, 1849, 1910
ScrFI CCNGG 8 cut(s) 644, 645, 742, 1293, 1314, 1860, 2081, 2372
SduI GDGCHC 4 cut(s) 221, 334, 1526, 2091
SfaNI GCATC 8 cut(s) 1213, 1668, 1730, 2141, 2197, 2215, 2371, 2528
SfcI CTRYAG 1 cut(s) 2715
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 5 cut(s) 85, 446, 1418, 1706, 2752
SmaI CCCGGG 1 cut(s) 645
SmiMI CAYNNNNRTG 1 cut(s) 1529
SmlI CTYRAG 2 cut(s) 703, 2678
SmoI CTYRAG 2 cut(s) 703, 2678
SsiI CCGC 9 cut(s) 25, 167, 181, 190, 204, 366, 1657, 2664, 2755
SspI AATATT 1 cut(s) 761
SspMI CTAG 7 cut(s) 282, 774, 855, 887, 1263, 1548, 2144
StyD4I CCNGG 8 cut(s) 642, 643, 740, 1291, 1312, 1858, 2079, 2370
StyI CCWWGG 5 cut(s) 735, 1503, 1612, 1920, 2323
TaaI ACNGT 7 cut(s) 406, 514, 630, 1669, 1769, 2497, 2789
TaiI ACGT 2 cut(s) 331, 885
TaqI TCGA 7 cut(s) 252, 422, 811, 1017, 2120, 2253, 2687
TaqII GACCGA 1 cut(s) 1454
TatI WGTACW 1 cut(s) 1034
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 7 cut(s) 312, 500, 934, 973, 1181, 2122, 2200
TscAI CASTG 7 cut(s) 688, 936, 1225, 1716, 1894, 1984, 2673
TseFI GTSAC 6 cut(s) 106, 199, 1714, 1750, 1913, 2738
Tsp45I GTSAC 6 cut(s) 106, 199, 1714, 1750, 1913, 2738
TspDTI ATGAA 9 cut(s) 17, 962, 1020, 1391, 1642, 2019, 2274, 2759, 2826
TspGWI ACGGA 6 cut(s) 64, 94, 238, 250, 1419, 2651
TspMI CCCGGG 1 cut(s) 643
TspRI CASTG 7 cut(s) 688, 936, 1225, 1716, 1894, 1984, 2673
Tth111I GACNNNGTC 3 cut(s) 197, 881, 1013
VpaK11BI GGWCC 5 cut(s) 85, 446, 1418, 1706, 2752
XagI CCTNNNNNAGG 1 cut(s) 1836
XapI RAATTY 3 cut(s) 836, 1796, 2113
XceI RCATGY 3 cut(s) 1345, 1691, 2188
XcmI CCANNNNNNNNNTGG 1 cut(s) 1548
XmaI CCCGGG 1 cut(s) 643
XmiI GTMKAC 2 cut(s) 252, 2817
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 7 cut(s) 282, 774, 855, 887, 1263, 1548, 2144
Zsp2I ATGCAT 2 cut(s) 2190, 2521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.