MD14G1217300.v1.1

WD repeat and HMG-box DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005318
Physical Location & Seq
Forward (+)
30085422 .. 30090270
4849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1217300.v1.1.491

Sequence Viewer

Length: 2892 bp
ATGAAGATCCGAACTTTGAAGCTCCGAGAGGCCCACAAGCCCACGAGCAGCGGCGGGGGGCCGTCGTACTGCTCGGTGCTGTGGGACCAGCAGGCCTACCACGTCGTGACGGCCTCCTCCTCCGACCCCACAATCGCCATCCACGACTCTCTTATCCTGTCCAGCCCTCCCAAGCTCCTCCGCCACCACCGCGACGGCGTCACGGCTCTCGCTCTCAGCCCCAACTCCACCTGCCTCGCCTCCGGCTCCGTCGACCACTCCGTCAAGCTCTACAAGTTTCCAGGTGGAGAATTTCAGACTAATATAACCCGATTCACGCTGCCAATACGCACCCTGGCGTTTAACAAATCCGGGACACTGTTGGCAGCTGCTGGTGATGATGAAGGCATTAAGCTTATTAACACAATTGATGGTTCAATTTCAAGAGTTCTTAAAGGACACAAAGGACCAGTGACAGGTTTAGGTTTCGATCCCCACAGTGAATATTTGGCCTCTATTGATTCAACTGGGGCTGTTATATACTGGGAACTTTCATCTGGAACCACCTTGCATACCCTTAAAGGGGTAGCTCCTAACTCGGGTTTCGATCATTCCATCATGAATGTACTTAGCTGGAGTCCTGATGGAGACAAGTTAGCCGTGCCAGGGTTGAGAAATGATGTGGTGGTTTATGATAGAGACACAGCTGAAAAGCTGTTTTCGTTGAGAGGTGATCACACACAGCCTATTTGTTCCTTGGCTTGGTCACCTAACGGAAAATACATGGCTACTTCTGGTTTGGATAAGCAGGTTCTCATCTGGGATGTTGATCGGAAGCAGGACATTGACAGGCAGAACTTTAATGAAAGGATATGTTGCTTGGCGTGGAAGCCCATTGGCAATGCACTGGCTGTTATTGATTGTATGGGAAAGTATGGCGTGTGGGATTCAGTGACTCCTTCATCAATGAAGTCGCCCACTGAAGATGTTCCCAATCTACAATCTAAGAACAGCAATGGGCTGCTTTTGTTTGACGAAGAGGAGGGTCAGGAGCTTAGCACTTCTGGTAGTTTAAGTGATGTTGGTGAAGATAGTCTTGGGGAATCCAAACCACCTAGCAGGAAGAGGCCACACAAGCACTCTGCATATGAGGATGATTTGGAAGAGGATGGTTTGGATGGCTTTAGGTTGATACCAAATGTTGAGTTGGAGTCCCGTAAAAAAGCGCGGCGTAAACACAGTGAAAGTGTTGATAATGAGAATGAGGGAGTAAGCAGCAAAGTGACATCGATTAGATCGAAAATGCAGGTGGCATTCCAGCCTGGTGCTACTCCTTTGCAGCCTGGAAAAAGGCGCTTTCTGTGCTATAACATGCTTGGAGCCATAACTACAGTTGAACATGACGGATTCTCCCATATTGAGATAGATTTTCATGATACAAGTCGCGGTCCAAGAGTTCCATCAATGAATGATTATTTTGGTTTCACAATGGCTTCACTTAATGAGAATGGAAGCGTTTTTGCAAATCCCTGCAAGGGAGAAAAGCACATGAGTACTCTCATGTATCGCCCATTCAGTACTTGGGCGAATAATAGTGAGTGGTCTATGCGATTGGAGGGGGAAGAAGTGAAGGTTGTGGCACTTGGCACATCTTGGGTGGCAGCAATTACGAGTCTTAACTTCCTTCGCATCTTCACTGAAAGTGGTTTGCAGAAACATGTTCTCTCCCTTGATGGACCTGTCGTGACCGCATCAGGCTTCAAGGATGAACTTGCGATTGTCACTCATGCTTCCGGTTGTCTTCCTTCAAATGAACAGATGCTTGAATTTAGAGTACTCAACATAACTAATGCAAGCCAGCCTCTTAGAGGACGGCTGCCTTTGACTCCTGGTTCACATTTAACATGGTTCGGGTTTAGTGAAGAAGGGCAATTAAGCTCCTACGATTCCAAGGGCGTGCTAAGAGTTTTTACAAGGCAATATGGTGGCAATTGGCTCCCACTCTTCAGTGCTAGCAAAGAGAAGAAGTCAGGCGAAAACTATTGGGTGGTTGGGTTGAATGCAAGCAAATTGTTTTGCATAGGTTGCAAAAGTCCTGACTTATACCCGCAGGTGATGCCGAAACCAGTCCTTACTCCACTAAATTTTTCGTTCCCTCTAGCATCCTCTGATCTAGGGGCAGAAGCCCTTGAAAATGAGTTTATACTAAACAACACGCACCTCGCACAGATTCAAAAGAAAATAGAAGAACTGGATGCTGCCGGTTTGGACACCACTTCACTTGACGATGAAGCTTTCAATACAGAAGCAGCTCAAGACAGATGCATCTTAAAGCTCATTGCATCCTGCTGCAACGGAGATAAGCTTGTGAGAGCAACTGAACTCGTGAAGCTTCTGTCCCTCGAAAAATCAGTAAGGGGTGCAATCAAGCTTGTCACTGTTCTGAAGCTCCCTAACTTGGCTGAAAGGTTCAACAGCATTTTGGAGGAAAGGCTGCTCAACGAGAGTAAAAGAGCTGCGGAAACCACCTTCCCTACCTCAAACCACAACGCATCTTTCATCACGGATGACATTGCAGCCGCAAAGAAAACAACCTCTACCGAGCCAAGTAAGTTGTCAGGAAACGACATTCCACTATCAACGCCAAAATTGTCGGCTCCTTTGTTTACGAAGAAAGCCAAAACACAAGAACCTAAAGTTGGAACAGAGAAAACAGACGAAAAACTGGCGGAGGATGATGTTGGGAAGGTAAAAACAACGGAGGCGAAAAATGCTACACAGGTGAACAGCAACGCAGGAGAGACCAAGAAGGTAGGGGAAGTGTCTGAGGTACCAAATGATCAAGAAAAAGTGAAGAAGGCAGAAGCACCTCGACGAGCATCTAATCCATTCATGAAGAAATTAATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

964

Amino Acids

105.39

Weight (kDa)

6.4

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 24 - 137 6.7e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 25 - 307 2.3e-71 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 32 - 139 5.3e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 34 - 187 8e-23 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 40 - 184 1.9e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 58 - 91 5.9e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 59 - 188 2.1e-18 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 62 - 273 1.6e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 213 7.8e-11 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 64 - 187 4.4e-08 WDR55
WD40_Prp19 PF24814 64 - 299 1.5e-30 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 64 - 188 4.2e-09 WDR3 second beta-propeller domain
Beta-prop_WDR19_1st PF23389 116 - 251 2.2e-09 WDR19 first beta-propeller
Beta-prop_WDR35_TULP_N PF24797 140 - 271 5.7e-06 WDR35/TULP4 N-terminal
Beta-prop_CAF1B_HIR1 PF24105 144 - 277 8.9e-08 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 151 - 293 1.5e-13 CDC20/Fizzy WD40 domain
Beta-prop_RIG_2nd PF23775 159 - 295 7.4e-06 RIG second beta-propeller
Beta-prop_IFT122_1st PF23381 199 - 313 3.5e-08 IFT122 first beta-propeller
Beta-prop_WDR3_1st PF25173 200 - 277 4.8e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 200 - 318 8.6e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 201 - 315 2.3e-11 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 203 - 278 4.9e-06 CAF1B/HIR1 beta-propeller domain
ANAPC4_WD40 PF12894 203 - 290 1.5e-07 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 210 - 300 5.1e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_Aladin PF25460 228 - 309 1.3e-07 Aladin seven-bladed propeller
WD40 PF00400 238 - 268 7.9e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 245 - 310 6.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Mcl1_mid PF12341 431 - 713 1.1e-94 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 722 - 825 2.2e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 239, 1276, 2080
AasI GACNNNNNNGTC 1 cut(s) 260
Acc36I ACCTGC 4 cut(s) 239, 778, 1276, 2080
Acc65I GGTACC 1 cut(s) 2809
AccB1I GGYRCC 1 cut(s) 2809
AccI GTMKAC 1 cut(s) 252
AccII CGCG 3 cut(s) 192, 1207, 1425
AclWI GGATC 1 cut(s) 464
AcsI RAATTY 3 cut(s) 290, 1805, 2122
AcuI CTGAAG 3 cut(s) 981, 1969, 2444
AcyI GRCGYC 1 cut(s) 198
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 6 cut(s) 68, 606, 1534, 1558, 1815, 2811
AflIII ACRYGT 1 cut(s) 1696
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
AjuI GAANNNNNNNTTGG 4 cut(s) 1059, 1091, 2661, 2693
Alw26I GTCTC 3 cut(s) 621, 672, 2774
AlwI GGATC 1 cut(s) 464
AlwNI CAGNNNCTG 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 577
AoxI GGCC 6 cut(s) 30, 59, 93, 111, 489, 1106
ApoI RAATTY 3 cut(s) 290, 1805, 2122
AseI ATTAAT 1 cut(s) 2882
Asp700I GAANNNNTTC 1 cut(s) 966
Asp718I GGTACC 1 cut(s) 2809
AspLEI GCGC 2 cut(s) 1207, 1335
AspS9I GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 6 cut(s) 386, 722, 738, 1076, 2104, 2773
AsuNHI GCTAGC 1 cut(s) 1991
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 4 cut(s) 85, 446, 1427, 1715
BanI GGYRCC 1 cut(s) 2809
BauI CACGAG 2 cut(s) 43, 2363
BbsI GAAGAC 1 cut(s) 1772
BccI CCATC 8 cut(s) 146, 404, 602, 617, 1142, 1151, 1447, 1706
BceAI ACGGC 6 cut(s) 46, 126, 211, 219, 623, 1868
BciT130I CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
BclI TGATCA 2 cut(s) 712, 2818
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 3 cut(s) 621, 672, 2774
BfaI CTAG 4 cut(s) 1095, 1992, 2138, 2153
BfmI CTRYAG 1 cut(s) 1368
BfoI RGCGCY 1 cut(s) 1336
BfuAI ACCTGC 4 cut(s) 239, 778, 1276, 2080
BlpI GCTNAGC 1 cut(s) 1034
BmcAI AGTACT 3 cut(s) 1534, 1558, 1815
Bme1390I CCNGG 7 cut(s) 282, 335, 352, 645, 1302, 1323, 1869
Bme18I GGWCC 4 cut(s) 85, 446, 1427, 1715
BmeT110I CYCGRG 1 cut(s) 577
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
BmiI GGNNCC 8 cut(s) 60, 86, 247, 541, 1360, 1976, 2637, 2811
BmrFI CCNGG 7 cut(s) 282, 335, 352, 645, 1302, 1323, 1869
BmrI ACTGGG 2 cut(s) 516, 532
BmtI GCTAGC 1 cut(s) 1995
BmuI ACTGGG 2 cut(s) 516, 532
BpiI GAAGAC 1 cut(s) 1772
BpmI CTGGAG 1 cut(s) 634
Bpu1102I GCTNAGC 1 cut(s) 1034
BpuEI CTTGAG 1 cut(s) 2277
BpuMI CCSGG 1 cut(s) 352
Bsa29I ATCGAT 1 cut(s) 1268
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 2774
BsaJI CCNNGG 4 cut(s) 333, 644, 735, 1929
BsaWI WCCGGW 1 cut(s) 1772
BsaXI ACNNNNNCTCC 2 cut(s) 1373, 1403
Bse118I RCCGGY 1 cut(s) 2240
Bse1I ACTGG 7 cut(s) 449, 511, 527, 891, 2105, 2235, 2709
Bse3DI GCAATG 4 cut(s) 886, 1000, 2316, 2550
Bse8I GATNNNNATC 1 cut(s) 807
BseBI CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
BseCI ATCGAT 1 cut(s) 1268
BseDI CCNNGG 4 cut(s) 333, 644, 735, 1929
BseJI GATNNNNATC 1 cut(s) 807
BseMI GCAATG 4 cut(s) 886, 1000, 2316, 2550
BseMII CTCAG 2 cut(s) 229, 2796
BseNI ACTGG 7 cut(s) 449, 511, 527, 891, 2105, 2235, 2709
BseRI GAGGAG 4 cut(s) 106, 109, 167, 1034
Bsh1236I CGCG 3 cut(s) 192, 1207, 1425
BshFI GGCC 6 cut(s) 32, 61, 95, 113, 491, 1108
BshNI GGYRCC 1 cut(s) 2809
BshVI ATCGAT 1 cut(s) 1268
BsiHKCI CYCGRG 1 cut(s) 577
BsiSI CCGG 4 cut(s) 243, 351, 1773, 2241
BslFI GGGAC 4 cut(s) 98, 367, 1177, 2362
BsmAI GTCTC 3 cut(s) 621, 672, 2774
BsmFI GGGAC 4 cut(s) 98, 367, 1177, 2362
BsmI GAATGC 2 cut(s) 1292, 2044
BsnI GGCC 6 cut(s) 32, 61, 95, 113, 491, 1108
Bso31I GGTCTC 1 cut(s) 2774
BsoBI CYCGRG 1 cut(s) 577
Bsp143I GATC 8 cut(s) 6, 469, 586, 712, 808, 1274, 2149, 2818
Bsp1720I GCTNAGC 1 cut(s) 1034
BspANI GGCC 6 cut(s) 32, 61, 95, 113, 491, 1108
BspCNI CTCAG 2 cut(s) 228, 2797
BspDI ATCGAT 1 cut(s) 1268
BspFNI CGCG 3 cut(s) 192, 1207, 1425
BspHI TCATGA 3 cut(s) 597, 1411, 2871
BspLI GGNNCC 8 cut(s) 60, 86, 247, 541, 1360, 1976, 2637, 2811
BspMI ACCTGC 4 cut(s) 239, 778, 1276, 2080
BspOI GCTAGC 1 cut(s) 1995
BspPI GGATC 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 2809
BspTNI GGTCTC 1 cut(s) 2774
BsrDI GCAATG 4 cut(s) 886, 1000, 2316, 2550
BsrFI RCCGGY 1 cut(s) 2240
BsrI ACTGG 7 cut(s) 449, 511, 527, 891, 2105, 2235, 2709
BssAI RCCGGY 1 cut(s) 2240
BssECI CCNNGG 4 cut(s) 333, 644, 735, 1929
BssMI GATC 8 cut(s) 6, 469, 586, 712, 808, 1274, 2149, 2818
BssNI GRCGYC 1 cut(s) 198
BssSI CACGAG 2 cut(s) 43, 2363
BssT1I CCWWGG 2 cut(s) 735, 1929
Bst2BI CACGAG 2 cut(s) 43, 2363
Bst2UI CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
Bst4CI ACNGT 5 cut(s) 360, 479, 1220, 1372, 2419
Bst6I CTCTTC 4 cut(s) 1011, 1097, 1137, 1988
BstACI GRCGYC 1 cut(s) 198
BstAPI GCANNNNNTGC 2 cut(s) 2064, 2095
BstC8I GCNNGC 6 cut(s) 93, 1834, 1838, 1937, 1993, 2044
BstDEI CTNAG 7 cut(s) 215, 608, 984, 1034, 1844, 1940, 2805
BstEII GGTNACC 1 cut(s) 744
BstENI CCTNNNNNAGG 1 cut(s) 1845
BstFNI CGCG 3 cut(s) 192, 1207, 1425
BstH2I RGCGCY 1 cut(s) 1336
BstHHI GCGC 2 cut(s) 1207, 1335
BstKTI GATC 8 cut(s) 9, 472, 589, 715, 811, 1277, 2152, 2821
BstMAI GTCTC 3 cut(s) 621, 672, 2774
BstMBI GATC 8 cut(s) 6, 469, 586, 712, 808, 1274, 2149, 2818
BstMWI GCNNNNNNNGC 6 cut(s) 189, 1114, 1341, 2064, 2095, 2750
BstNI CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
BstNSI RCATGY 2 cut(s) 1354, 1700
BstPI GGTNACC 1 cut(s) 744
BstSCI CCNGG 7 cut(s) 280, 333, 350, 643, 1300, 1321, 1867
BstSFI CTRYAG 1 cut(s) 1368
BstUI CGCG 3 cut(s) 192, 1207, 1425
BstV2I GAAGAC 1 cut(s) 1772
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
Bsu15I ATCGAT 1 cut(s) 1268
BsuRI GGCC 6 cut(s) 32, 61, 95, 113, 491, 1108
BsuTUI ATCGAT 1 cut(s) 1268
BtrI CACGTC 1 cut(s) 103
BveI ACCTGC 4 cut(s) 239, 778, 1276, 2080
Cac8I GCNNGC 6 cut(s) 93, 1834, 1838, 1937, 1993, 2044
CaiI CAGNNNCTG 1 cut(s) 371
CciI TCATGA 3 cut(s) 597, 1411, 2871
CfoI GCGC 2 cut(s) 1207, 1335
Cfr10I RCCGGY 1 cut(s) 2240
Cfr13I GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
ClaI ATCGAT 1 cut(s) 1268
CseI GACGC 1 cut(s) 187
Csp6I GTAC 6 cut(s) 67, 605, 1533, 1557, 1814, 2810
CspCI CAANNNNNGTGG 2 cut(s) 2242, 2277
CviQI GTAC 6 cut(s) 67, 605, 1533, 1557, 1814, 2810
DdeI CTNAG 7 cut(s) 215, 608, 984, 1034, 1844, 1940, 2805
DpnI GATC 8 cut(s) 8, 471, 588, 714, 810, 1276, 2151, 2820
DpnII GATC 8 cut(s) 6, 469, 586, 712, 808, 1274, 2149, 2818
DraIII CACNNNGTG 1 cut(s) 106
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 4 cut(s) 1011, 1097, 1137, 1988
EarI CTCTTC 4 cut(s) 1011, 1097, 1137, 1988
EciI GGCGGA 2 cut(s) 170, 2723
Eco130I CCWWGG 2 cut(s) 735, 1929
Eco147I AGGCCT 1 cut(s) 95
Eco31I GGTCTC 1 cut(s) 2774
Eco47I GGWCC 4 cut(s) 85, 446, 1427, 1715
Eco57I CTGAAG 3 cut(s) 981, 1969, 2444
Eco88I CYCGRG 1 cut(s) 577
Eco91I GGTNACC 1 cut(s) 744
EcoNI CCTNNNNNAGG 1 cut(s) 1845
EcoO65I GGTNACC 1 cut(s) 744
EcoRII CCWGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
EcoT14I CCWWGG 2 cut(s) 735, 1929
EcoT22I ATGCAT 1 cut(s) 2306
ErhI CCWWGG 2 cut(s) 735, 1929
FalI AAGNNNNNCTT 2 cut(s) 1059, 1091
FaqI GGGAC 4 cut(s) 98, 367, 1177, 2362
FauI CCCGC 2 cut(s) 47, 2094
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 2 cut(s) 712, 2818
FblI GTMKAC 1 cut(s) 252
FspBI CTAG 4 cut(s) 1095, 1992, 2138, 2153
GlaI GCGC 2 cut(s) 1206, 1334
GsuI CTGGAG 1 cut(s) 634
HaeII RGCGCY 1 cut(s) 1336
HaeIII GGCC 6 cut(s) 32, 61, 95, 113, 491, 1108
HapII CCGG 4 cut(s) 243, 351, 1773, 2241
HgaI GACGC 1 cut(s) 187
HhaI GCGC 2 cut(s) 1207, 1335
Hin1I GRCGYC 1 cut(s) 198
Hin6I GCGC 2 cut(s) 1205, 1333
HinP1I GCGC 2 cut(s) 1205, 1333
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 5 cut(s) 392, 2271, 2342, 2369, 2408
HpaII CCGG 4 cut(s) 243, 351, 1773, 2241
HphI GGTGA 6 cut(s) 386, 722, 738, 1076, 2104, 2773
Hpy166II GTNNAC 5 cut(s) 253, 1214, 1874, 2646, 2764
Hpy188I TCNGA 8 cut(s) 11, 26, 124, 297, 813, 2149, 2424, 2806
Hpy8I GTNNAC 5 cut(s) 253, 1214, 1874, 2646, 2764
Hpy99I CGWCG 5 cut(s) 67, 107, 197, 254, 2856
HpyCH4III ACNGT 5 cut(s) 360, 479, 1220, 1372, 2419
HpyCH4IV ACGT 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 6 cut(s) 189, 1114, 1341, 2064, 2095, 2750
HpyF3I CTNAG 7 cut(s) 215, 608, 984, 1034, 1844, 1940, 2805
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 198
HspAI GCGC 2 cut(s) 1205, 1333
KpnI GGTACC 1 cut(s) 2813
Ksp22I TGATCA 2 cut(s) 712, 2818
Kzo9I GATC 8 cut(s) 6, 469, 586, 712, 808, 1274, 2149, 2818
MaeI CTAG 4 cut(s) 1095, 1992, 2138, 2153
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 9 cut(s) 106, 199, 451, 744, 931, 1261, 1723, 1759, 2413
MalI GATC 8 cut(s) 8, 471, 588, 714, 810, 1276, 2151, 2820
MboI GATC 8 cut(s) 6, 469, 586, 712, 808, 1274, 2149, 2818
MfeI CAATTG 2 cut(s) 405, 1969
MflI RGATCY 1 cut(s) 6
MlyI GAGTC 6 cut(s) 140, 625, 928, 1199, 1660, 1858
MmeI TCCRAC 3 cut(s) 147, 1167, 2659
Mph1103I ATGCAT 1 cut(s) 2306
MroXI GAANNNNTTC 1 cut(s) 966
MspA1I CMGCKG 3 cut(s) 51, 368, 686
MspI CCGG 4 cut(s) 243, 351, 1773, 2241
MspR9I CCNGG 7 cut(s) 282, 335, 352, 645, 1302, 1323, 1869
MunI CAATTG 2 cut(s) 405, 1969
Mva1269I GAATGC 2 cut(s) 1292, 2044
MvaI CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
MvnI CGCG 3 cut(s) 192, 1207, 1425
MwoI GCNNNNNNNGC 6 cut(s) 189, 1114, 1341, 2064, 2095, 2750
NciI CCSGG 1 cut(s) 352
NdeI CATATG 1 cut(s) 1126
NdeII GATC 8 cut(s) 6, 469, 586, 712, 808, 1274, 2149, 2818
NheI GCTAGC 1 cut(s) 1991
NlaIV GGNNCC 8 cut(s) 60, 86, 247, 541, 1360, 1976, 2637, 2811
NmuCI GTSAC 9 cut(s) 106, 199, 451, 744, 931, 1261, 1723, 1759, 2413
NsiI ATGCAT 1 cut(s) 2306
NspI RCATGY 2 cut(s) 1354, 1700
PacI TTAATTAA 1 cut(s) 2886
PagI TCATGA 3 cut(s) 597, 1411, 2871
PaqCI CACCTGC 3 cut(s) 239, 1276, 2080
PceI AGGCCT 1 cut(s) 95
PciI ACATGT 1 cut(s) 1696
PcsI WCGNNNNNNNCGW 3 cut(s) 141, 258, 1274
PctI GAATGC 2 cut(s) 1292, 2044
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 7 cut(s) 312, 500, 926, 1082, 1386, 1925, 2209
PflFI GACNNNGTC 1 cut(s) 197
PfoI TCCNGGA 1 cut(s) 350
PleI GAGTC 6 cut(s) 140, 624, 928, 1198, 1659, 1858
PpsI GAGTC 6 cut(s) 140, 624, 928, 1198, 1659, 1858
PscI ACATGT 1 cut(s) 1696
PshBI ATTAAT 1 cut(s) 2882
Psp6I CCWGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
PspEI GGTNACC 1 cut(s) 744
PspGI CCWGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
PspN4I GGNNCC 8 cut(s) 60, 86, 247, 541, 1360, 1976, 2637, 2811
PspPI GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 6
PsyI GACNNNGTC 1 cut(s) 197
PvuII CAGCTG 2 cut(s) 368, 686
RsaI GTAC 6 cut(s) 68, 606, 1534, 1558, 1815, 2811
RsaNI GTAC 6 cut(s) 67, 605, 1533, 1557, 1814, 2810
SalI GTCGAC 1 cut(s) 251
Sau3AI GATC 8 cut(s) 6, 469, 586, 712, 808, 1274, 2149, 2818
Sau96I GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
ScaI AGTACT 3 cut(s) 1534, 1558, 1815
SchI GAGTC 6 cut(s) 140, 625, 928, 1199, 1660, 1858
ScrFI CCNGG 7 cut(s) 282, 335, 352, 645, 1302, 1323, 1869
SfcI CTRYAG 1 cut(s) 1368
SinI GGWCC 4 cut(s) 85, 446, 1427, 1715
SmlI CTYRAG 1 cut(s) 2292
SmoI CTYRAG 1 cut(s) 2292
SseBI AGGCCT 1 cut(s) 95
SspI AATATT 1 cut(s) 485
SspMI CTAG 4 cut(s) 1095, 1992, 2138, 2153
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 7 cut(s) 280, 333, 350, 643, 1300, 1321, 1867
StyI CCWWGG 2 cut(s) 735, 1929
TaaI ACNGT 5 cut(s) 360, 479, 1220, 1372, 2419
TaiI ACGT 1 cut(s) 105
TaqI TCGA 7 cut(s) 252, 468, 585, 1268, 1277, 2382, 2851
TatI WGTACW 4 cut(s) 604, 1532, 1556, 1813
TauI GCSGC 3 cut(s) 54, 1210, 2561
TfiI GAWTC 7 cut(s) 312, 500, 926, 1082, 1386, 1925, 2209
TseFI GTSAC 9 cut(s) 106, 199, 451, 744, 931, 1261, 1723, 1759, 2413
Tsp45I GTSAC 9 cut(s) 106, 199, 451, 744, 931, 1261, 1723, 1759, 2413
TspGWI ACGGA 7 cut(s) 238, 250, 768, 1398, 2349, 2558, 2753
Tth111I GACNNNGTC 1 cut(s) 197
VpaK11BI GGWCC 4 cut(s) 85, 446, 1427, 1715
VspI ATTAAT 1 cut(s) 2882
XagI CCTNNNNNAGG 1 cut(s) 1845
XapI RAATTY 3 cut(s) 290, 1805, 2122
XceI RCATGY 2 cut(s) 1354, 1700
XcmI CCANNNNNNNNNTGG 1 cut(s) 1557
XmiI GTMKAC 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 4 cut(s) 1095, 1992, 2138, 2153
ZrmI AGTACT 3 cut(s) 1534, 1558, 1815
Zsp2I ATGCAT 1 cut(s) 2306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.