Rh7AG062100

WD repeat and HMG-box DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0005318
Physical Location & Seq
Forward (+)
4329025 .. 4334090
5066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG062100.1

Sequence Viewer

Length: 2895 bp
ATGAAGGTCCGGCAAGTGAAGCTCCGCGAGATCCACAAATCGGCGAGCAGCGCCGGAAAGCCGTCCTTCTGCTCCGTCGTGTGGGACCAGCAAGGCTTCCATTCCGTCACCGCCTCCTCCTCCGACTCCTCGATCGCCGTCCACAACGCTCTTCTCTCCACATGTCCGCCGAAGCTCCTCCGCAACCACCGCGACGGCGTCACGGCGCTCGCTATGAGCCCCAACGCCACCTGCCTCGCCTCCGGCTCCGTCGACCACTCCGTCAAGCTCTACAAGTTTCCTAGTGGAGAGTTCGAGACTAATATTACCAGATTCACGCTGCCAATACGTGCCCTTGCGTTTAATAAGTCAGGGACACTCTTGGCCGCTGCTGGTGATGATGATGGCATTAAACTTATTAACACAGTGGATGGTACAATCTCGAAGGTTCTCAAAGCACACAAAGGACCTGTAACCGGCTTAGGTTTTGATCCCCAAAGTGAATACATGGCATCCATCGATTCAACTGGGACTCTAGTATACTGGGAACTCCAATCTGGAAGCACATTGCACACCCTTAAAGGCGTAGCTCCTAACTCTGGTTTAGACCTTTCTGTATTGAATGTACTGAGCTGGAGTCCTGATGGACGGATGTTAGCTGTTCCGGGAGTGAGAAATGATGTTGTGGTGTATGATAAAGACACTGCTGAGAAGCTCTTTACATTGAAAGGTGAACATACACAGTCTATTTGTGCCTTGGCTTGGTCACCTAATGGAAAATACTTGGCTACCTCTGGCTTGGATAAGCAGGTTCTCATCTGGGATGTTGATCGGAAGCAGGATATTGGCAGGCATAAATTTGATGAGAGGATAACTTGCTTGGCGTGGAAGCCAATTGACAATGTCCTAGCTGTAATTGATTGTATGGGCAAGTATGGTGTGTGGGAAGCAGTGATTCCTTCATCAATGAAATCTCCCACTGAAGATGTTCCGAATCAATCTAATAATGGTCTACTTTTGTTTGATGAAGACGAAGACGAGGGTCAGGAGATTAGTACATCTGGTAGTTTAAGTGATCTTGGTGAAGATACATATGAGGAGTCTAAGCCACCTAGCCGGAAAAGACTAAGCAAGCAGTCTGACTCTCTGAGAGATTTAAATGAGGATGATAATGATGACTTTGGTCTGTCCCCTAAAGTTGAATCCCATAAAAAAGCACGTCGGAATCATAGTGAAAGCACTGACAATGGGAATGAGGGATTAAGAAGCAGACTGTCATCTGCCAGGCCAAAAATGCAGGAGGCATTTCAGCCAGGGGCCACTCCTGTGCAGCCTGGAAAAAGGCGCTTTTTGTGCTACAACATGCTTGGAGCTATAACTACGATTGAATACGATGGGTACTCCCATATTGAGATAGATTTTCATGATACAAGCCGAGGTCCACGAGTTCCATCAATGACAGACCATTTTGGTTTTACAATGGCTTCACTGAATGAGAATGGAAGTGTTTTTGCAAATCCCTGCAAGGGTGAAAAGAACATGAGCACTCTCATGTATCGCCCATTTAGTAGCTGGGCAAACAATAGCGAGTGGTCTATGCGATTGGAAGGGGAAGAAGTGAAGGTTGTGGCACTTGGTACAACTTGGGTGGCAGCAATTACTAGTCTTAACTTGATCCGCATCTTCACCATTGGTGGTTTGCAGAGACATGTTCTCTCCCTCGATGGACCTGTGGTGACTGCATCAGGCTTCATGGATGAGCTTGCCATTGTCACTCATGCTTCAGACAGTCTTCCCTCAAATGAACAGGTGCTTGAATTTAGGGTACTTAACATAAATAATAGTAGCCAGCCTCTTAGAGGACGTTTGCCTCTGACTCCTGGTTCAAGTTTAACATGGTTTGGCTTTAGTGAAGAAGGGAAATTAAGCTCCTATGACTCCAAGGGTGTGCTGAGAGTTTTTACAAGTGAATATGGTGGCAGTTGGTTCCCGCTCTTCAGTGCTAGTAAAGACAAGAAGTCAGATGAAAACTATTGGGTGATCGGTTTGAATGCAAGACAACTATTTTGTATAGTTTGCAAAAGCCCCGAGTTATATCCACAGGTGTTGCCCAAACCAGTGCTCACTCTACTAAATTTTTCAATTCCTCTTGCATCCTCTGATCTAGGAGCAGAAGCCCTTGAAAATGCATTTATACTAAACGACATGCATCTCGTACAGATTCAAAAGCAAATAGAAGAAATGGATGCTGTAGGTTTAGACACCAGTTCACTTGATGATGAAGCTTTTAAATTGGAAACAGCTCAAGATCAATGTATATTGAGGCTTATTGGATCCTGCTGCCATGGAGATAAGCTTGTGAGGGCTACTGAACTTGTGAAGCATCTATCCCTGGAAAAATCAGTTAGGGGTGCAATTAAGATTGTTACTTCTCTGAAGCTACCTAACTTGGCTGAAAGGTTCAATAGCATATTGGAGGAAAGGTTGCTCAACGATAGTAAAGAACCATTGCAAACAACTGTCCCAAACCCAAACTACAATGCATCAACCTCTACCGAGAAAAGTAAGTTATCAGGGAATGATATTCCATTACCAGCACCAAAATTGTCAGCTCCTTCGTTTCTAAAGAAAGCCAAAACACAAGAAGCTAATGTTGGAACAGCGAAAACACAAGAAAAACAAACTGATCTTGAGAAGTCGACGACGACTGAGAGGGAAACTGCTGGTGCAGAGGTGAATAATGCAGCAGGTGACATGAAGAGGGTAGGGGAAATGCCTAAGGCACAACAGTCACCTCGCCCATCTAATCCATTTCTAAAGCGATCAAACAGTCAAGACATAGAGAAGAAGGAAGAAGTAGATCAGGTGAAAGCTCACCGCCCATCGAATCCGTTCTTCAAGAAATCGGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

964

Amino Acids

105.84

Weight (kDa)

6.06

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 9 - 138 7.2e-15 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 22 - 188 4.6e-13 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 25 - 307 2.5e-69 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 26 - 187 1e-22 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 37 - 184 8.2e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 46 - 188 1.4e-18 WDR3 first beta-propeller domain
WD40 PF00400 59 - 91 5.5e-06 WD domain, G-beta repeat
Beta-prop_EML PF23409 62 - 280 9.2e-11 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 198 2.4e-09 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 64 - 298 1.5e-32 Prp19 WD40 domain
WDR55 PF24796 64 - 187 9.6e-10 WDR55
Beta-prop_WDR3_2nd PF25172 64 - 189 1e-10 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 108 - 269 4.6e-09 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 116 - 269 3.1e-13 WDR19 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 145 - 276 2.1e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 146 - 300 1e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_IFT122_1st PF23381 200 - 310 4.9e-08 IFT122 first beta-propeller
Beta-prop_WDR3_1st PF25173 201 - 289 1.1e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 201 - 300 6.5e-15 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 201 - 310 4.4e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT140_1st PF23383 201 - 285 1.4e-07 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 202 - 298 3.8e-12 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 203 - 296 2.4e-13 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 203 - 277 1.4e-06 CAF1B/HIR1 beta-propeller domain
ANAPC4_WD40 PF12894 203 - 290 1.5e-07 Anaphase-promoting complex subunit 4 WD40 domain
WD40 PF00400 232 - 268 2.3e-08 WD domain, G-beta repeat
Mcl1_mid PF12341 427 - 710 4.9e-95 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 720 - 822 2.7e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 239, 2717
AasI GACNNNNNNGTC 1 cut(s) 260
Acc36I ACCTGC 3 cut(s) 239, 778, 2717
AccBSI CCGCTC 1 cut(s) 1972
AccI GTMKAC 4 cut(s) 252, 519, 991, 2678
AccII CGCG 2 cut(s) 27, 192
AciI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2857
AclWI GGATC 5 cut(s) 25, 464, 1648, 2307, 2320
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 3 cut(s) 836, 1796, 2113
AcuI CTGAAG 4 cut(s) 981, 1746, 1960, 2435
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 7 cut(s) 415, 606, 1036, 1379, 1618, 1806, 2196
AfiI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
AflIII ACRYGT 2 cut(s) 161, 1687
AhdI GACNNNNNGTC 1 cut(s) 1996
AhlI ACTAGT 1 cut(s) 1640
AjiI CACGTC 1 cut(s) 1199
AjnI CCWGG 5 cut(s) 1262, 1291, 1312, 1858, 2370
AjuI GAANNNNNNNTTGG 2 cut(s) 2616, 2648
AleI CACNNNNGTG 1 cut(s) 1304
Alw21I GWGCWC 2 cut(s) 1526, 2103
Alw26I GTCTC 2 cut(s) 290, 1678
AlwI GGATC 5 cut(s) 25, 464, 1648, 2307, 2320
Ama87I CYCGRG 1 cut(s) 2066
AoxI GGCC 3 cut(s) 363, 1265, 1296
ApeKI GCWGC 7 cut(s) 48, 319, 368, 1309, 1631, 2319, 2723
ApoI RAATTY 3 cut(s) 836, 1796, 2113
ArsI GACNNNNNNTTYG 2 cut(s) 2028, 2060
Asp700I GAANNNNTTC 2 cut(s) 966, 2870
AspLEI GCGC 3 cut(s) 53, 208, 1326
AspS9I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
AsuC2I CCSGG 1 cut(s) 645
AvaI CYCGRG 1 cut(s) 2066
AvaII GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
AxyI CCTNAGG 1 cut(s) 2757
BaeGI GKGCMC 1 cut(s) 334
BamHI GGATCC 1 cut(s) 2312
BanII GRGCYC 1 cut(s) 221
BauI CACGAG 1 cut(s) 1422
BbsI GAAGAC 3 cut(s) 1014, 1020, 1763
Bbv12I GWGCWC 2 cut(s) 1526, 2103
BbvI GCAGC 7 cut(s) 60, 306, 355, 1321, 1643, 2306, 2735
BccI CCATC 9 cut(s) 377, 404, 503, 617, 1367, 1438, 1697, 2788, 2869
BceAI ACGGC 4 cut(s) 46, 122, 211, 219
BciT130I CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2372
BcnI CCSGG 1 cut(s) 645
BcoDI GTCTC 2 cut(s) 290, 1678
BcuI ACTAGT 1 cut(s) 1640
BfaI CTAG 7 cut(s) 282, 515, 887, 1092, 1641, 1983, 2144
BfmI CTRYAG 1 cut(s) 2229
BfoI RGCGCY 3 cut(s) 54, 209, 1327
BfuAI ACCTGC 3 cut(s) 239, 778, 2717
BisI GCNGC 8 cut(s) 49, 320, 366, 369, 1310, 1632, 2320, 2724
BlsI GCNGC 8 cut(s) 50, 321, 367, 370, 1311, 1633, 2321, 2725
Bme1390I CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
Bme18I GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
BmeRI GACNNNNNGTC 1 cut(s) 1996
BmeT110I CYCGRG 1 cut(s) 2066
BmgBI CACGTC 1 cut(s) 1199
BmgT120I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
BmiI GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2314
BmrFI CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
BmrI ACTGGG 2 cut(s) 516, 532
BmsI GCATC 8 cut(s) 500, 1668, 1730, 2141, 2197, 2215, 2371, 2531
BmuI ACTGGG 2 cut(s) 516, 532
BoxI GACNNNNGTC 2 cut(s) 1020, 1161
BpiI GAAGAC 3 cut(s) 1014, 1020, 1763
BpmI CTGGAG 1 cut(s) 634
Bpu10I CCTNAGC 1 cut(s) 460
BpuEI CTTGAG 2 cut(s) 2268, 2690
BpuMI CCSGG 1 cut(s) 645
Bsa29I ATCGAT 1 cut(s) 498
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 2 cut(s) 807, 1658
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2323, 2370
BsaXI ACNNNNNCTCC 2 cut(s) 2447, 2477
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 4 cut(s) 511, 527, 2096, 2244
Bse21I CCTNAGG 1 cut(s) 2757
Bse3DI GCAATG 2 cut(s) 545, 2486
Bse8I GATNNNNATC 2 cut(s) 807, 1658
BseBI CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2372
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2323, 2370
BseGI GGATG 8 cut(s) 415, 491, 636, 808, 1150, 1741, 2132, 2230
BseJI GATNNNNATC 2 cut(s) 807, 1658
BseLI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
BseMI GCAATG 2 cut(s) 545, 2486
BseMII CTCAG 5 cut(s) 599, 678, 1118, 1922, 2679
BseNI ACTGG 4 cut(s) 511, 527, 2096, 2244
BseRI GAGGAG 5 cut(s) 106, 109, 118, 167, 1091
BseSI GKGCMC 1 cut(s) 334
BseXI GCAGC 7 cut(s) 60, 306, 355, 1321, 1643, 2306, 2735
BseYI CCCAGC 1 cut(s) 1551
BsgI GTGCAG 2 cut(s) 1328, 2727
Bsh1236I CGCG 2 cut(s) 27, 192
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 3 cut(s) 365, 1267, 1298
BshVI ATCGAT 1 cut(s) 498
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 1526, 2103
BsiHKCI CYCGRG 1 cut(s) 2066
BsiSI CCGG 6 cut(s) 10, 54, 243, 456, 644, 1096
BslFI GGGAC 5 cut(s) 98, 367, 523, 1153, 2486
BslI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
BsmAI GTCTC 2 cut(s) 290, 1678
BsmFI GGGAC 5 cut(s) 98, 367, 523, 1153, 2486
BsmI GAATGC 1 cut(s) 2035
BsnI GGCC 3 cut(s) 365, 1267, 1298
BsoBI CYCGRG 1 cut(s) 2066
Bsp1286I GDGCHC 4 cut(s) 221, 334, 1526, 2103
Bsp19I CCATGG 1 cut(s) 2323
BspACI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2857
BspANI GGCC 3 cut(s) 365, 1267, 1298
BspCNI CTCAG 5 cut(s) 600, 679, 1119, 1923, 2680
BspDI ATCGAT 1 cut(s) 498
BspFNI CGCG 2 cut(s) 27, 192
BspHI TCATGA 1 cut(s) 1402
BspLI GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2314
BspMI ACCTGC 3 cut(s) 239, 778, 2717
BspPI GGATC 5 cut(s) 25, 464, 1648, 2307, 2320
BspQI GCTCTTC 2 cut(s) 156, 1979
BsrBI CCGCTC 1 cut(s) 1972
BsrDI GCAATG 2 cut(s) 545, 2486
BsrFI RCCGGY 1 cut(s) 455
BsrI ACTGG 4 cut(s) 511, 527, 2096, 2244
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2323, 2370
BssNAI GTATAC 1 cut(s) 520
BssNI GRCGYC 1 cut(s) 198
BssSI CACGAG 1 cut(s) 1422
BssT1I CCWWGG 3 cut(s) 735, 1920, 2323
Bst1107I GTATAC 1 cut(s) 520
Bst2BI CACGAG 1 cut(s) 1422
Bst2UI CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2372
Bst4CI ACNGT 7 cut(s) 406, 723, 1254, 1769, 2500, 2769, 2810
Bst6I CTCTTC 3 cut(s) 156, 1979, 2732
BstACI GRCGYC 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 6 cut(s) 46, 210, 830, 1112, 1743, 1829
BstDSI CCRYGG 1 cut(s) 2323
BstEII GGTNACC 1 cut(s) 744
BstENI CCTNNNNNAGG 1 cut(s) 1836
BstF5I GGATG 8 cut(s) 415, 491, 636, 808, 1150, 1741, 2132, 2230
BstFNI CGCG 2 cut(s) 27, 192
BstH2I RGCGCY 3 cut(s) 54, 209, 1327
BstHHI GCGC 3 cut(s) 53, 208, 1326
BstMAI GTCTC 2 cut(s) 290, 1678
BstMCI CGRYCG 1 cut(s) 135
BstMWI GCNNNNNNNGC 5 cut(s) 19, 50, 189, 1273, 1332
BstNI CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2372
BstNSI RCATGY 4 cut(s) 165, 1345, 1691, 2188
BstPAI GACNNNNGTC 2 cut(s) 1020, 1161
BstPI GGTNACC 1 cut(s) 744
BstSCI CCNGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2370
BstSFI CTRYAG 1 cut(s) 2229
BstSLI GKGCMC 1 cut(s) 334
BstUI CGCG 2 cut(s) 27, 192
BstV1I GCAGC 7 cut(s) 60, 306, 355, 1321, 1643, 2306, 2735
BstV2I GAAGAC 3 cut(s) 1014, 1020, 1763
BstX2I RGATCY 2 cut(s) 30, 2312
BstYI RGATCY 2 cut(s) 30, 2312
BstZ17I GTATAC 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 498
Bsu36I CCTNAGG 1 cut(s) 2757
BsuRI GGCC 3 cut(s) 365, 1267, 1298
BsuTUI ATCGAT 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 2323
BtrI CACGTC 1 cut(s) 1199
BtsCI GGATG 8 cut(s) 415, 491, 636, 808, 1150, 1741, 2132, 2230
BtsI GCAGTG 2 cut(s) 681, 936
BtsIMutI CAGTG 8 cut(s) 411, 681, 936, 957, 1218, 1466, 1984, 2103
BveI ACCTGC 3 cut(s) 239, 778, 2717
Cac8I GCNNGC 6 cut(s) 46, 210, 830, 1112, 1743, 1829
CciI TCATGA 1 cut(s) 1402
CfoI GCGC 3 cut(s) 53, 208, 1326
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
ClaI ATCGAT 1 cut(s) 498
CseI GACGC 1 cut(s) 187
Csp6I GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2195
CspCI CAANNNNNGTGG 4 cut(s) 1608, 1643, 2067, 2102
CviQI GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2195
DraI TTTAAA 2 cut(s) 1137, 2269
DrdI GACNNNNNNGTC 1 cut(s) 260
DriI GACNNNNNGTC 1 cut(s) 1996
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 3 cut(s) 156, 1979, 2732
Eam1105I GACNNNNNGTC 1 cut(s) 1996
EarI CTCTTC 3 cut(s) 156, 1979, 2732
EciI GGCGGA 1 cut(s) 156
Eco130I CCWWGG 3 cut(s) 735, 1920, 2323
Eco24I GRGCYC 1 cut(s) 221
Eco47I GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
Eco57I CTGAAG 4 cut(s) 981, 1746, 1960, 2435
Eco81I CCTNAGG 1 cut(s) 2757
Eco88I CYCGRG 1 cut(s) 2066
Eco91I GGTNACC 1 cut(s) 744
EcoNI CCTNNNNNAGG 1 cut(s) 1836
EcoO109I RGGNCCY 1 cut(s) 446
EcoO65I GGTNACC 1 cut(s) 744
EcoRII CCWGG 5 cut(s) 1262, 1291, 1312, 1858, 2370
EcoT14I CCWWGG 3 cut(s) 735, 1920, 2323
EcoT22I ATGCAT 3 cut(s) 2170, 2190, 2524
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 3 cut(s) 735, 1920, 2323
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 5 cut(s) 98, 367, 523, 1153, 2486
FauI CCCGC 1 cut(s) 1977
FauNDI CATATG 1 cut(s) 1072
FblI GTMKAC 4 cut(s) 252, 519, 991, 2678
Fnu4HI GCNGC 8 cut(s) 49, 320, 366, 369, 1310, 1632, 2320, 2724
FokI GGATG 8 cut(s) 422, 478, 643, 815, 1157, 1748, 2119, 2237
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 8 cut(s) 49, 320, 366, 369, 1310, 1632, 2320, 2724
FspBI CTAG 7 cut(s) 282, 515, 887, 1092, 1641, 1983, 2144
GlaI GCGC 3 cut(s) 52, 207, 1325
GluI GCNGC 8 cut(s) 49, 320, 366, 369, 1310, 1632, 2320, 2724
GsaI CCCAGC 1 cut(s) 1555
GsuI CTGGAG 1 cut(s) 634
HaeII RGCGCY 3 cut(s) 54, 209, 1327
HaeIII GGCC 3 cut(s) 365, 1267, 1298
HapII CCGG 6 cut(s) 10, 54, 243, 456, 644, 1096
HgaI GACGC 1 cut(s) 187
HhaI GCGC 3 cut(s) 53, 208, 1326
Hin1I GRCGYC 1 cut(s) 198
Hin6I GCGC 3 cut(s) 51, 206, 1324
HinP1I GCGC 3 cut(s) 51, 206, 1324
HincII GTYRAC 2 cut(s) 253, 2679
HindII GTYRAC 2 cut(s) 253, 2679
HindIII AAGCTT 2 cut(s) 2262, 2333
HpaII CCGG 6 cut(s) 10, 54, 243, 456, 644, 1096
Hpy166II GTNNAC 8 cut(s) 142, 253, 520, 713, 992, 1421, 2249, 2679
Hpy8I GTNNAC 8 cut(s) 142, 253, 520, 713, 992, 1421, 2249, 2679
Hpy99I CGWCG 6 cut(s) 80, 197, 254, 1203, 2683, 2686
HpyAV CCTTC 8 cut(s) 76, 418, 948, 1580, 1594, 1889, 2604, 2821
HpyCH4III ACNGT 7 cut(s) 406, 723, 1254, 1769, 2500, 2769, 2810
HpyCH4IV ACGT 3 cut(s) 328, 1198, 1843
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 50, 189, 1273, 1332
HpySE526I ACGT 3 cut(s) 328, 1198, 1843
Hsp92I GRCGYC 1 cut(s) 198
HspAI GCGC 3 cut(s) 51, 206, 1324
LguI GCTCTTC 2 cut(s) 156, 1979
LmnI GCTCC 9 cut(s) 27, 77, 180, 251, 574, 1349, 1913, 2147, 2596
Lsp1109I GCAGC 7 cut(s) 60, 306, 355, 1321, 1643, 2306, 2735
LweI GCATC 8 cut(s) 500, 1668, 1730, 2141, 2197, 2215, 2371, 2531
MaeI CTAG 7 cut(s) 282, 515, 887, 1092, 1641, 1983, 2144
MaeII ACGT 3 cut(s) 328, 1198, 1843
MaeIII GTNAC 9 cut(s) 106, 199, 451, 744, 1714, 1750, 2404, 2729, 2769
MbiI CCGCTC 1 cut(s) 1972
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 2 cut(s) 30, 2312
MhlI GDGCHC 4 cut(s) 221, 334, 1526, 2103
MlyI GAGTC 7 cut(s) 119, 505, 625, 1088, 1115, 1849, 1910
MmeI TCCRAC 3 cut(s) 147, 1181, 2614
Mph1103I ATGCAT 3 cut(s) 2170, 2190, 2524
MroXI GAANNNNTTC 2 cut(s) 966, 2870
MslI CAYNNNNRTG 2 cut(s) 1304, 1529
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 6 cut(s) 10, 54, 243, 456, 644, 1096
MspR9I CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 2035
MvaI CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2372
MvnI CGCG 2 cut(s) 27, 192
MwoI GCNNNNNNNGC 5 cut(s) 19, 50, 189, 1273, 1332
NciI CCSGG 1 cut(s) 645
NcoI CCATGG 1 cut(s) 2323
NdeI CATATG 1 cut(s) 1072
NlaIV GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2314
NmeAIII GCCGAG 1 cut(s) 1439
NmuCI GTSAC 7 cut(s) 106, 199, 744, 1714, 1750, 2729, 2769
NsiI ATGCAT 3 cut(s) 2170, 2190, 2524
NspI RCATGY 4 cut(s) 165, 1345, 1691, 2188
OliI CACNNNNGTG 1 cut(s) 1304
PagI TCATGA 1 cut(s) 1402
PaqCI CACCTGC 2 cut(s) 239, 2717
PciI ACATGT 2 cut(s) 161, 1687
PciSI GCTCTTC 2 cut(s) 156, 1979
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 1 cut(s) 2035
PdmI GAANNNNTTC 2 cut(s) 966, 2870
PfeI GAWTC 8 cut(s) 312, 500, 934, 973, 1181, 1204, 2200, 2866
PflFI GACNNNGTC 2 cut(s) 197, 881
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 8 cut(s) 50, 321, 367, 370, 1311, 1633, 2321, 2725
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 7 cut(s) 119, 505, 624, 1087, 1115, 1849, 1910
PpsI GAGTC 7 cut(s) 119, 505, 624, 1087, 1115, 1849, 1910
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 2 cut(s) 161, 1687
PshAI GACNNNNGTC 2 cut(s) 1020, 1161
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 5 cut(s) 1262, 1291, 1312, 1858, 2370
PspEI GGTNACC 1 cut(s) 744
PspFI CCCAGC 1 cut(s) 1551
PspGI CCWGG 5 cut(s) 1262, 1291, 1312, 1858, 2370
PspN4I GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2314
PspPI GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
PspPPI RGGWCCY 1 cut(s) 446
PsuI RGATCY 2 cut(s) 30, 2312
PsyI GACNNNGTC 2 cut(s) 197, 881
PvuI CGATCG 1 cut(s) 135
RsaI GTAC 7 cut(s) 415, 606, 1036, 1379, 1618, 1806, 2196
RsaNI GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2195
RseI CAYNNNNRTG 2 cut(s) 1304, 1529
SalI GTCGAC 2 cut(s) 251, 2677
SapI GCTCTTC 2 cut(s) 156, 1979
SatI GCNGC 8 cut(s) 49, 320, 366, 369, 1310, 1632, 2320, 2724
Sau96I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
SchI GAGTC 7 cut(s) 119, 505, 625, 1088, 1115, 1849, 1910
ScrFI CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
SduI GDGCHC 4 cut(s) 221, 334, 1526, 2103
SfaNI GCATC 8 cut(s) 500, 1668, 1730, 2141, 2197, 2215, 2371, 2531
SfcI CTRYAG 1 cut(s) 2229
SinI GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
SmiI ATTTAAAT 1 cut(s) 1137
SmiMI CAYNNNNRTG 2 cut(s) 1304, 1529
SmlI CTYRAG 2 cut(s) 2283, 2669
SmoI CTYRAG 2 cut(s) 2283, 2669
SpeI ACTAGT 1 cut(s) 1640
SsiI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2857
SspI AATATT 1 cut(s) 304
SspMI CTAG 7 cut(s) 282, 515, 887, 1092, 1641, 1983, 2144
StyD4I CCNGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2370
StyI CCWWGG 3 cut(s) 735, 1920, 2323
SwaI ATTTAAAT 1 cut(s) 1137
TaaI ACNGT 7 cut(s) 406, 723, 1254, 1769, 2500, 2769, 2810
TaiI ACGT 3 cut(s) 331, 1201, 1846
TaqI TCGA 8 cut(s) 131, 252, 294, 422, 498, 1701, 2678, 2864
TaqII GACCGA 1 cut(s) 2875
TatI WGTACW 2 cut(s) 604, 1034
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 8 cut(s) 312, 500, 934, 973, 1181, 1204, 2200, 2866
TscAI CASTG 8 cut(s) 411, 688, 936, 964, 1225, 1473, 1984, 2103
TseFI GTSAC 7 cut(s) 106, 199, 744, 1714, 1750, 2729, 2769
TseI GCWGC 7 cut(s) 48, 319, 368, 1309, 1631, 2319, 2723
Tsp45I GTSAC 7 cut(s) 106, 199, 744, 1714, 1750, 2729, 2769
TspGWI ACGGA 6 cut(s) 64, 94, 238, 250, 643, 2859
TspRI CASTG 8 cut(s) 411, 688, 936, 964, 1225, 1473, 1984, 2103
Tth111I GACNNNGTC 2 cut(s) 197, 881
VpaK11BI GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
XagI CCTNNNNNAGG 1 cut(s) 1836
XapI RAATTY 3 cut(s) 836, 1796, 2113
XceI RCATGY 4 cut(s) 165, 1345, 1691, 2188
XcmI CCANNNNNNNNNTGG 1 cut(s) 1548
XmiI GTMKAC 4 cut(s) 252, 519, 991, 2678
XmnI GAANNNNTTC 2 cut(s) 966, 2870
XspI CTAG 7 cut(s) 282, 515, 887, 1092, 1641, 1983, 2144
Zsp2I ATGCAT 3 cut(s) 2170, 2190, 2524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.