RLG00000005014

WD repeat and HMG-box DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005318
Physical Location & Seq
Reverse (-)
64349760 .. 64354411
4652 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005014

Sequence Viewer

Length: 2892 bp
ATGAAGGTCCGGCAAGTGAAGCTCCGCGAGATCCACAAATCGGCGAGCAGCGCCGGAAAGCCGTCCTTCTGCTCCGTCGTGTGGGACCAGCAAGGCTTCCACTCCGTCACCGCCTCCTCCTCCGACGCCTCAATCGCCGTCCACAACGCTCTTCTCTCCACATGTCCGCCGAAGCTCCTCCGCAACCACCGCGACGGCGTCACGGCGCTCGCTATGAGCCCCAACGCCACCTGCCTCGCCTCCGGCTCCGTCGACCACTCCGTCAAGCTCTACAAGTTTCCTAGTGGAGAGTTTGAGACTAATATTACCAGATTCACGCTGCCAATACGTGCCCTTGCGTTCAATAAGTCAGGGACACTCTTGGCCGCTGCTGGTGATGATGATGGCATTAAACTTATTAACACAGTAGATGGTACAATTTCGAAGGTTCTCAAAGCACACAAAGGACCTGTAACCGGCTTAGGTTTTGATCCCCAAAGTGAATACATGGCATCCATCGATTCAACTGGGACTGTAGTATACTGGGAACTCCAATCTGGAAGCACATTGCACACCCTTAAAGGCGTAGCTCCTAACTCTGGTTTAGACCTTTCTGTATTGAATGTACTGAGCTGGAGTCCTGATGGACGGATGTTAGCTGTTCCGGGAGTGAGAAATGATGTTGTGGTGTATGATAAAGACACTGCTGAGAAGCTCTTTACATTGAGAGGTGAACATACACAGTCTATTTGTGCCTTGGCTTGGTCACCTAATGGAAAATACTTGGCTACCTCTGGCTTGGATAAGCAGGTTCTCATCTGGGATGTTGATCGGAAGCAGGATATTGGCAGGCATAAATTTGATGAGAGGATAACTTGCTTGGCGTGGAAGCCAATTGACAATGTCCTAGCTGTAATTGATTGTATGGGCAGGTATGGTGTGTGGGAAGCAGTGATTCCTTCATCAATGAAATCTCCCACTGAAGATGTTCCGAATCAATCTAATAATGGTCTACTTTTGTTTGATGAAGACGAAGACGAGGGTCAGGATATTAGTACATCTGGTAGTTTAAGTGATCTTGGTGAAGATACATATGAGGAGTCTAAGCCACCTAGCCGGAAAAGACTAAGCAAGCAGTCTGACTCTCTGAGAGATTTAGATGAGGATGATAATGATGACTTTGGTCTGTCCCCTAAAGTTGAATCCCATAAAAAAGCACGTCGAAATCATAGTGAAAGCACTGACAATGGGAATGAGGGATTAAGAAGCAGACTGTCATCTGCCAGGCCAAAAATGCAGGAGGCATTTCAGCCAGGGGCCACTCCTGTGCAGCCTGGAAAAAGGCGCTTTTTGTGCTACAACATGCTTGGAGCTATAACTACGATTGAATACGATGGGTACTCCCATATTGAGATAGATTTTCATGATACAAGCCGAGGTCCACGAGTTCCATCAATGACAGACCATTTTGGTTTTACAATGGCTTCACTGAATGAGAATGGAAGTGTTTTTGCAAATCCCTGCAAGGGTGAAAAGAACATGAGCACTCTCATGTATCGGCCATTTAGTAGCTGGGCAAACAATAGCGAGTGGTCTATGCGATTGGAAGGGGAAGAAGTGAAGGTTGTGGCACTTGGTACAACTTGGGTGGCAGCAATTACTAGTCTTAACTTGATCCGCATCTTCACCATTGGTGGTTTGCAGAGACATGTTCTCTCCCTCGATGGACCTGTGGTGACTGCATCAGGCTTTATGGATGAGCTTGCCATTGTCACTCATGCTTCAGACAGTCTACCCTCAAATGAACAGGTGCTTGAATTTAGGGTACTTAACATAAATAATAGTAGCCAGCCTCTTAGAGGACGTCTGCCTCTGACTCCTGGTTCAAGTTTAACATGGTTTGGCTTTAGTGAAGAAGGGAAATTAAGCTCCTATGACTCCAAGGGTGTGCTGAGAGTTTTTACAAGTGAATATGGTGGCAGTTGGTTCCCGCTCTTCAGTGCTAGTAAAGACAAGTCAGATGAAAACTATTGGGTGATCGGTTTGAATGCAAGACAACTATTTTGTATAGTTTGCAAAAGCCCCGAATTATATCCACAGGTGTTGCCCAAACCAGTGCTCACTCTACTAAATTTTTCAATTCCTCTTGCATCGTCTGATCTAGGAGCAGAAGCCCTTGAAAATGCATTTATACTAAACGACATGCATCTCGTACAGATTCAAAAGCAAATAGAAGAAATGGATGCTGCAGGTTTAGACACCAGTTCACTTGATGATGAAGCTTTTAAATTGGAAACAGCTCAAGATCAATGTATATTGAGGCTTATTGGATCCTGCTGCCATGGAGATAAGCTTGTGAGGGCTACTGAACTTGTGAAGCATCTATCCCTGGAAAAATCAGTTAGGGGTGCAATTAAGATTGTTACTTCTCTGAAGCTACCTAACTTGGCTGAAAGGTTCAATAGCATATTGGAGGAAAGGTTGCTCAACGATAGTAAAGAGCCATTGCAAACAACTGTCCCAAACCCAAACTACAATGCATCATCCTCTACCGAGAAAAGTAAGTTATCAGGGAATGATATTCCATTACCAGCACCAAAATTGTCAGCTCCTACGTTTCTAAAGAAAGCCAAAACACAAGAAGCTAATGTTGGAACAGCGAAAACACAAGAAAAGCAAACTGATCTTGAGAAGTCGACGACGACTGAGAGGGAAACTGCTGGTGCAGATGTGAATAATGCAGCAGGTGACATGAAGAAGGTAGGGGAAATGCCTAAGGCACAACAGTCACATCGCCCAACTAATCCATTTCTAAAGCGATCAAACAGTCAAGACATAGAGAAGAAGGAAGAAGTAGATCAGGTGAAAGCTCACCGCCCATCGAATCCGTTCTTCAAGAAATCGGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

964

Amino Acids

105.71

Weight (kDa)

5.97

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 9 - 138 1e-14 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 22 - 187 7.3e-13 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 25 - 307 3.3e-69 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 25 - 187 4e-23 WDR5 beta-propeller domain
WD40_Gbeta PF25391 36 - 157 6e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 37 - 184 1.9e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 59 - 188 7.7e-19 WDR3 first beta-propeller domain
WD40 PF00400 59 - 91 5.5e-06 WD domain, G-beta repeat
Beta-prop_EML PF23409 62 - 280 1.8e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 198 9e-10 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 64 - 187 3.9e-10 WDR55
WD40_Prp19 PF24814 64 - 298 2.3e-32 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 64 - 188 4.5e-11 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 107 - 269 1.4e-09 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 116 - 269 2.1e-13 WDR19 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 144 - 275 7e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 171 - 295 7.4e-12 WDR3 first beta-propeller domain
Beta-prop_IFT122_1st PF23381 200 - 310 2e-07 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 201 - 299 9.4e-15 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 201 - 285 2.1e-07 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 202 - 297 5.2e-12 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 203 - 290 2e-07 Anaphase-promoting complex subunit 4 WD40 domain
WD40_CDC20-Fz PF24807 203 - 296 3.1e-13 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 204 - 277 1.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 205 - 299 5.2e-08 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 232 - 268 2.7e-08 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 232 - 290 7.2e-06 TEP-1 second beta-propeller
Beta-prop_Aladin PF25460 233 - 309 7.4e-06 Aladin seven-bladed propeller
Mcl1_mid PF12341 427 - 709 2.4e-94 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 719 - 821 2.1e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 239, 2714
AasI GACNNNNNNGTC 1 cut(s) 260
AatII GACGTC 1 cut(s) 1846
Acc36I ACCTGC 5 cut(s) 239, 778, 900, 2219, 2714
AccBSI CCGCTC 1 cut(s) 1972
AccI GTMKAC 5 cut(s) 252, 519, 991, 1771, 2675
AccII CGCG 2 cut(s) 27, 192
AciI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2854
AclWI GGATC 5 cut(s) 25, 464, 1648, 2304, 2317
AcoI YGGCCR 2 cut(s) 363, 1538
AcsI RAATTY 3 cut(s) 836, 1796, 2110
AcuI CTGAAG 4 cut(s) 981, 1746, 1960, 2432
AcyI GRCGYC 3 cut(s) 126, 198, 1843
AfaI GTAC 7 cut(s) 415, 606, 1036, 1379, 1618, 1806, 2193
AfiI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
AflIII ACRYGT 2 cut(s) 161, 1687
AhlI ACTAGT 1 cut(s) 1640
AjiI CACGTC 1 cut(s) 1199
AjnI CCWGG 5 cut(s) 1262, 1291, 1312, 1858, 2367
AjuI GAANNNNNNNTTGG 2 cut(s) 2613, 2645
AleI CACNNNNGTG 1 cut(s) 1304
Alw21I GWGCWC 2 cut(s) 1526, 2100
Alw26I GTCTC 2 cut(s) 290, 1678
AlwI GGATC 5 cut(s) 25, 464, 1648, 2304, 2317
AoxI GGCC 4 cut(s) 363, 1265, 1296, 1538
ApeKI GCWGC 8 cut(s) 48, 319, 368, 1309, 1631, 2225, 2316, 2720
ApoI RAATTY 3 cut(s) 836, 1796, 2110
ArsI GACNNNNNNTTYG 2 cut(s) 2025, 2057
Asp700I GAANNNNTTC 2 cut(s) 966, 2867
AspLEI GCGC 3 cut(s) 53, 208, 1326
AspS9I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
AsuC2I CCSGG 1 cut(s) 645
AsuII TTCGAA 1 cut(s) 422
AvaII GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
AxyI CCTNAGG 1 cut(s) 2754
BaeGI GKGCMC 1 cut(s) 334
BamHI GGATCC 1 cut(s) 2309
BanII GRGCYC 1 cut(s) 221
BauI CACGAG 1 cut(s) 1422
BbsI GAAGAC 2 cut(s) 1014, 1020
Bbv12I GWGCWC 2 cut(s) 1526, 2100
BbvI GCAGC 8 cut(s) 60, 306, 355, 1321, 1643, 2212, 2303, 2732
BccI CCATC 8 cut(s) 377, 404, 503, 617, 1367, 1438, 1697, 2866
BceAI ACGGC 4 cut(s) 46, 122, 211, 219
BciT130I CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2369
BcnI CCSGG 1 cut(s) 645
BcoDI GTCTC 2 cut(s) 290, 1678
BcuI ACTAGT 1 cut(s) 1640
BfaI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2141
BfmI CTRYAG 2 cut(s) 513, 2226
BfoI RGCGCY 3 cut(s) 54, 209, 1327
BfuAI ACCTGC 5 cut(s) 239, 778, 900, 2219, 2714
BisI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2226, 2317, 2721
BlsI GCNGC 9 cut(s) 50, 321, 367, 370, 1311, 1633, 2227, 2318, 2722
Bme1390I CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2369
Bme18I GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
BmgBI CACGTC 1 cut(s) 1199
BmgT120I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
BmiI GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2311
BmrFI CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2369
BmrI ACTGGG 2 cut(s) 516, 532
BmsI GCATC 8 cut(s) 500, 1668, 1730, 2138, 2194, 2212, 2368, 2528
BmuI ACTGGG 2 cut(s) 516, 532
BoxI GACNNNNGTC 2 cut(s) 1020, 1161
BpiI GAAGAC 2 cut(s) 1014, 1020
BpmI CTGGAG 1 cut(s) 634
Bpu10I CCTNAGC 1 cut(s) 460
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 2 cut(s) 2265, 2687
BpuMI CCSGG 1 cut(s) 645
Bsa29I ATCGAT 1 cut(s) 498
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 2 cut(s) 807, 1658
BsaHI GRCGYC 3 cut(s) 126, 198, 1843
BsaJI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2320, 2367
BsaXI ACNNNNNCTCC 2 cut(s) 2444, 2474
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 4 cut(s) 511, 527, 2093, 2241
Bse21I CCTNAGG 1 cut(s) 2754
Bse3DI GCAATG 2 cut(s) 545, 2483
Bse8I GATNNNNATC 2 cut(s) 807, 1658
BseBI CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2369
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2320, 2367
BseGI GGATG 7 cut(s) 491, 636, 808, 1150, 1741, 2227, 2522
BseJI GATNNNNATC 2 cut(s) 807, 1658
BseLI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
BseMI GCAATG 2 cut(s) 545, 2483
BseMII CTCAG 5 cut(s) 599, 678, 1118, 1922, 2676
BseNI ACTGG 4 cut(s) 511, 527, 2093, 2241
BseRI GAGGAG 4 cut(s) 106, 109, 167, 1091
BseSI GKGCMC 1 cut(s) 334
BseXI GCAGC 8 cut(s) 60, 306, 355, 1321, 1643, 2212, 2303, 2732
BseYI CCCAGC 1 cut(s) 1551
BsgI GTGCAG 2 cut(s) 1328, 2724
Bsh1236I CGCG 2 cut(s) 27, 192
BshFI GGCC 4 cut(s) 365, 1267, 1298, 1540
BshVI ATCGAT 1 cut(s) 498
BsiHKAI GWGCWC 2 cut(s) 1526, 2100
BsiSI CCGG 6 cut(s) 10, 54, 243, 456, 644, 1096
BslFI GGGAC 5 cut(s) 98, 367, 523, 1153, 2483
BslI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
BsmAI GTCTC 2 cut(s) 290, 1678
BsmFI GGGAC 5 cut(s) 98, 367, 523, 1153, 2483
BsmI GAATGC 1 cut(s) 2032
BsnI GGCC 4 cut(s) 365, 1267, 1298, 1540
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 4 cut(s) 221, 334, 1526, 2100
Bsp19I CCATGG 1 cut(s) 2320
BspACI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2854
BspANI GGCC 4 cut(s) 365, 1267, 1298, 1540
BspCNI CTCAG 5 cut(s) 600, 679, 1119, 1923, 2677
BspDI ATCGAT 1 cut(s) 498
BspFNI CGCG 2 cut(s) 27, 192
BspHI TCATGA 1 cut(s) 1402
BspLI GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2311
BspMAI CTGCAG 1 cut(s) 2230
BspMI ACCTGC 5 cut(s) 239, 778, 900, 2219, 2714
BspPI GGATC 5 cut(s) 25, 464, 1648, 2304, 2317
BspQI GCTCTTC 2 cut(s) 156, 1979
BspT104I TTCGAA 1 cut(s) 422
BsrBI CCGCTC 1 cut(s) 1972
BsrDI GCAATG 2 cut(s) 545, 2483
BsrFI RCCGGY 1 cut(s) 455
BsrI ACTGG 4 cut(s) 511, 527, 2093, 2241
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2320, 2367
BssNAI GTATAC 1 cut(s) 520
BssNI GRCGYC 3 cut(s) 126, 198, 1843
BssSI CACGAG 1 cut(s) 1422
BssT1I CCWWGG 3 cut(s) 735, 1920, 2320
Bst1107I GTATAC 1 cut(s) 520
Bst2BI CACGAG 1 cut(s) 1422
Bst2UI CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2369
Bst4CI ACNGT 8 cut(s) 406, 514, 723, 1254, 1769, 2497, 2766, 2807
Bst6I CTCTTC 2 cut(s) 156, 1979
BstACI GRCGYC 3 cut(s) 126, 198, 1843
BstBAI YACGTR 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 6 cut(s) 46, 210, 830, 1112, 1743, 1829
BstDSI CCRYGG 1 cut(s) 2320
BstEII GGTNACC 1 cut(s) 744
BstENI CCTNNNNNAGG 1 cut(s) 1836
BstF5I GGATG 7 cut(s) 491, 636, 808, 1150, 1741, 2227, 2522
BstFNI CGCG 2 cut(s) 27, 192
BstH2I RGCGCY 3 cut(s) 54, 209, 1327
BstHHI GCGC 3 cut(s) 53, 208, 1326
BstMAI GTCTC 2 cut(s) 290, 1678
BstMWI GCNNNNNNNGC 6 cut(s) 19, 50, 134, 189, 1273, 1332
BstNI CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2369
BstNSI RCATGY 4 cut(s) 165, 1345, 1691, 2185
BstPAI GACNNNNGTC 2 cut(s) 1020, 1161
BstPI GGTNACC 1 cut(s) 744
BstSCI CCNGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2367
BstSFI CTRYAG 2 cut(s) 513, 2226
BstSLI GKGCMC 1 cut(s) 334
BstUI CGCG 2 cut(s) 27, 192
BstV1I GCAGC 8 cut(s) 60, 306, 355, 1321, 1643, 2212, 2303, 2732
BstV2I GAAGAC 2 cut(s) 1014, 1020
BstX2I RGATCY 2 cut(s) 30, 2309
BstYI RGATCY 2 cut(s) 30, 2309
BstZ17I GTATAC 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 498
Bsu36I CCTNAGG 1 cut(s) 2754
BsuRI GGCC 4 cut(s) 365, 1267, 1298, 1540
BsuTUI ATCGAT 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 2320
BtgZI GCGATG 1 cut(s) 2756
BtrI CACGTC 1 cut(s) 1199
BtsCI GGATG 7 cut(s) 491, 636, 808, 1150, 1741, 2227, 2522
BtsI GCAGTG 2 cut(s) 681, 936
BtsIMutI CAGTG 7 cut(s) 681, 936, 957, 1218, 1466, 1984, 2100
BveI ACCTGC 5 cut(s) 239, 778, 900, 2219, 2714
Cac8I GCNNGC 6 cut(s) 46, 210, 830, 1112, 1743, 1829
CciI TCATGA 1 cut(s) 1402
CfoI GCGC 3 cut(s) 53, 208, 1326
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
ClaI ATCGAT 1 cut(s) 498
CseI GACGC 2 cut(s) 134, 187
Csp6I GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2192
CspCI CAANNNNNGTGG 4 cut(s) 1608, 1643, 2064, 2099
CviQI GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2192
DraI TTTAAA 1 cut(s) 2266
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 2 cut(s) 363, 1538
Eam1104I CTCTTC 2 cut(s) 156, 1979
EarI CTCTTC 2 cut(s) 156, 1979
EciI GGCGGA 1 cut(s) 156
Eco130I CCWWGG 3 cut(s) 735, 1920, 2320
Eco24I GRGCYC 1 cut(s) 221
Eco47I GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
Eco57I CTGAAG 4 cut(s) 981, 1746, 1960, 2432
Eco81I CCTNAGG 1 cut(s) 2754
Eco91I GGTNACC 1 cut(s) 744
EcoNI CCTNNNNNAGG 1 cut(s) 1836
EcoO109I RGGNCCY 1 cut(s) 446
EcoO65I GGTNACC 1 cut(s) 744
EcoRII CCWGG 5 cut(s) 1262, 1291, 1312, 1858, 2367
EcoT14I CCWWGG 3 cut(s) 735, 1920, 2320
EcoT22I ATGCAT 3 cut(s) 2167, 2187, 2521
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 3 cut(s) 735, 1920, 2320
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 5 cut(s) 98, 367, 523, 1153, 2483
FauI CCCGC 1 cut(s) 1977
FauNDI CATATG 1 cut(s) 1072
FblI GTMKAC 5 cut(s) 252, 519, 991, 1771, 2675
Fnu4HI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2226, 2317, 2721
FokI GGATG 7 cut(s) 478, 643, 815, 1157, 1748, 2234, 2509
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2226, 2317, 2721
FspBI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2141
GlaI GCGC 3 cut(s) 52, 207, 1325
GluI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2226, 2317, 2721
GsaI CCCAGC 1 cut(s) 1555
GsuI CTGGAG 1 cut(s) 634
HaeII RGCGCY 3 cut(s) 54, 209, 1327
HaeIII GGCC 4 cut(s) 365, 1267, 1298, 1540
HapII CCGG 6 cut(s) 10, 54, 243, 456, 644, 1096
HgaI GACGC 2 cut(s) 134, 187
HhaI GCGC 3 cut(s) 53, 208, 1326
Hin1I GRCGYC 3 cut(s) 126, 198, 1843
Hin6I GCGC 3 cut(s) 51, 206, 1324
HinP1I GCGC 3 cut(s) 51, 206, 1324
HincII GTYRAC 2 cut(s) 253, 2676
HindII GTYRAC 2 cut(s) 253, 2676
HindIII AAGCTT 2 cut(s) 2259, 2330
HpaII CCGG 6 cut(s) 10, 54, 243, 456, 644, 1096
Hpy166II GTNNAC 9 cut(s) 142, 253, 520, 713, 992, 1421, 1772, 2246, 2676
Hpy188III TCNNGA 8 cut(s) 537, 620, 1025, 1403, 2282, 2666, 2810, 2875
Hpy8I GTNNAC 9 cut(s) 142, 253, 520, 713, 992, 1421, 1772, 2246, 2676
Hpy99I CGWCG 7 cut(s) 80, 128, 197, 254, 1203, 2680, 2683
HpyAV CCTTC 8 cut(s) 76, 418, 948, 1580, 1594, 1889, 2731, 2818
HpyCH4III ACNGT 8 cut(s) 406, 514, 723, 1254, 1769, 2497, 2766, 2807
HpyCH4IV ACGT 4 cut(s) 328, 1198, 1843, 2594
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 50, 134, 189, 1273, 1332
HpySE526I ACGT 4 cut(s) 328, 1198, 1843, 2594
Hsp92I GRCGYC 3 cut(s) 126, 198, 1843
HspAI GCGC 3 cut(s) 51, 206, 1324
LguI GCTCTTC 2 cut(s) 156, 1979
LmnI GCTCC 9 cut(s) 27, 77, 180, 251, 574, 1349, 1913, 2144, 2593
Lsp1109I GCAGC 8 cut(s) 60, 306, 355, 1321, 1643, 2212, 2303, 2732
LweI GCATC 8 cut(s) 500, 1668, 1730, 2138, 2194, 2212, 2368, 2528
MaeI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2141
MaeII ACGT 4 cut(s) 328, 1198, 1843, 2594
MaeIII GTNAC 9 cut(s) 106, 199, 451, 744, 1714, 1750, 2401, 2726, 2766
MbiI CCGCTC 1 cut(s) 1972
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 2 cut(s) 30, 2309
MhlI GDGCHC 4 cut(s) 221, 334, 1526, 2100
MlyI GAGTC 5 cut(s) 625, 1088, 1115, 1849, 1910
MmeI TCCRAC 2 cut(s) 147, 2611
Mph1103I ATGCAT 3 cut(s) 2167, 2187, 2521
MroXI GAANNNNTTC 2 cut(s) 966, 2867
MslI CAYNNNNRTG 2 cut(s) 1304, 1529
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 6 cut(s) 10, 54, 243, 456, 644, 1096
MspR9I CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2369
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 2032
MvaI CCWGG 5 cut(s) 1264, 1293, 1314, 1860, 2369
MvnI CGCG 2 cut(s) 27, 192
MwoI GCNNNNNNNGC 6 cut(s) 19, 50, 134, 189, 1273, 1332
NciI CCSGG 1 cut(s) 645
NcoI CCATGG 1 cut(s) 2320
NdeI CATATG 1 cut(s) 1072
NlaIV GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2311
NmeAIII GCCGAG 1 cut(s) 1439
NmuCI GTSAC 7 cut(s) 106, 199, 744, 1714, 1750, 2726, 2766
NsiI ATGCAT 3 cut(s) 2167, 2187, 2521
NspI RCATGY 4 cut(s) 165, 1345, 1691, 2185
NspV TTCGAA 1 cut(s) 422
OliI CACNNNNGTG 1 cut(s) 1304
PagI TCATGA 1 cut(s) 1402
PaqCI CACCTGC 2 cut(s) 239, 2714
PciI ACATGT 2 cut(s) 161, 1687
PciSI GCTCTTC 2 cut(s) 156, 1979
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 1 cut(s) 2032
PdmI GAANNNNTTC 2 cut(s) 966, 2867
PfeI GAWTC 7 cut(s) 312, 500, 934, 973, 1181, 2197, 2863
PflFI GACNNNGTC 2 cut(s) 197, 881
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 9 cut(s) 50, 321, 367, 370, 1311, 1633, 2227, 2318, 2722
PleI GAGTC 5 cut(s) 624, 1087, 1115, 1849, 1910
PpsI GAGTC 5 cut(s) 624, 1087, 1115, 1849, 1910
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 2 cut(s) 161, 1687
PshAI GACNNNNGTC 2 cut(s) 1020, 1161
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 5 cut(s) 1262, 1291, 1312, 1858, 2367
PspEI GGTNACC 1 cut(s) 744
PspFI CCCAGC 1 cut(s) 1551
PspGI CCWGG 5 cut(s) 1262, 1291, 1312, 1858, 2367
PspN4I GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2311
PspPI GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
PspPPI RGGWCCY 1 cut(s) 446
PstI CTGCAG 1 cut(s) 2230
PsuI RGATCY 2 cut(s) 30, 2309
PsyI GACNNNGTC 2 cut(s) 197, 881
RsaI GTAC 7 cut(s) 415, 606, 1036, 1379, 1618, 1806, 2193
RsaNI GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2192
RseI CAYNNNNRTG 2 cut(s) 1304, 1529
SalI GTCGAC 2 cut(s) 251, 2674
SapI GCTCTTC 2 cut(s) 156, 1979
SatI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2226, 2317, 2721
Sau96I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
SchI GAGTC 5 cut(s) 625, 1088, 1115, 1849, 1910
ScrFI CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2369
SduI GDGCHC 4 cut(s) 221, 334, 1526, 2100
SfaNI GCATC 8 cut(s) 500, 1668, 1730, 2138, 2194, 2212, 2368, 2528
SfcI CTRYAG 2 cut(s) 513, 2226
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
SmiMI CAYNNNNRTG 2 cut(s) 1304, 1529
SmlI CTYRAG 2 cut(s) 2280, 2666
SmoI CTYRAG 2 cut(s) 2280, 2666
SpeI ACTAGT 1 cut(s) 1640
SsiI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2854
SspI AATATT 1 cut(s) 304
SspMI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2141
StyD4I CCNGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2367
StyI CCWWGG 3 cut(s) 735, 1920, 2320
TaaI ACNGT 8 cut(s) 406, 514, 723, 1254, 1769, 2497, 2766, 2807
TaiI ACGT 4 cut(s) 331, 1201, 1846, 2597
TaqI TCGA 7 cut(s) 252, 422, 498, 1201, 1701, 2675, 2861
TaqII GACCGA 1 cut(s) 2872
TatI WGTACW 2 cut(s) 604, 1034
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 7 cut(s) 312, 500, 934, 973, 1181, 2197, 2863
TscAI CASTG 7 cut(s) 688, 936, 964, 1225, 1473, 1984, 2100
TseFI GTSAC 7 cut(s) 106, 199, 744, 1714, 1750, 2726, 2766
TseI GCWGC 8 cut(s) 48, 319, 368, 1309, 1631, 2225, 2316, 2720
Tsp45I GTSAC 7 cut(s) 106, 199, 744, 1714, 1750, 2726, 2766
TspDTI ATGAA 9 cut(s) 17, 930, 962, 1020, 1391, 1797, 2016, 2271, 2747
TspGWI ACGGA 6 cut(s) 64, 94, 238, 250, 643, 2856
TspRI CASTG 7 cut(s) 688, 936, 964, 1225, 1473, 1984, 2100
Tth111I GACNNNGTC 2 cut(s) 197, 881
VpaK11BI GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
XagI CCTNNNNNAGG 1 cut(s) 1836
XapI RAATTY 3 cut(s) 836, 1796, 2110
XceI RCATGY 4 cut(s) 165, 1345, 1691, 2185
XcmI CCANNNNNNNNNTGG 1 cut(s) 1548
XmiI GTMKAC 5 cut(s) 252, 519, 991, 1771, 2675
XmnI GAANNNNTTC 2 cut(s) 966, 2867
XspI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2141
ZraI GACGTC 1 cut(s) 1844
Zsp2I ATGCAT 3 cut(s) 2167, 2187, 2521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.