Rh7DG062100

WD repeat and HMG-box DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0005318
Physical Location & Seq
Forward (+)
4799014 .. 4804076
5063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG062100.1

Sequence Viewer

Length: 2409 bp
ATGGCATCCATCGATTCAACTGGGACTGTAGTATATTGGGAACTCCAATCTGGAAGCACATTGCACACCCTTAAAGGCGTAGCTCCTAACTCTGGTTTAGACCTTTCTGTATTGAATGTACTGAGCTGGAGTCCTGATGGACGGATGTTAGCTGTTCCGGGAGTGAGAAATGATGTTGTGGTGTATGATAAAGACACTGCTGAGAAGCTCTTTACATTGAGAGGTGAACATACACAGTCTATTTGTGCCTTGGCTTGGTCACCTAATGGAAAATACTTGGCTACCTCTGGCTTGGATAAGCAGGTTCTCATCTGGGATGTTGATCGGAAGCAGGATATTGGCAGACATAAATTTGATGAGAGGATAACTTGCTTGGCGTGGAAGCCAATTGACAATGTCCTAGCTGTAATTGATTGTATGGGCAGGTATGGTGTGTGGGAAGCAGTGATTCCTTCATCAATGAAATCTCCCACTGAAGATGTTCCGAATCAATCTAATAATGGTCTACTTTTGTTTGATGAAGACGAAGACGAGGGTCAGGAGATTAGTACATCTGGTAGTTTAAGTGATCTTGGTGAAGATACATATGAGGAGTCTAAGCCACCTAGCCGGAAAAGACTAAGCAAGCAGTCTGACTCTCTGAGAGATTTAAATGAGGATGATAATGATGACTTTGGTCTGTCCCCTAAAGTTGAATCCCATAAAAAAGCACGTCGGAATCATAGTGAAAGCACTGACAATGGGAATGAGGGATTAAGAAGCAGACTGTCATCTGCCAGGCCAAAAATGCAGGAGGCATTTCAGCCAGGGGCCACTCCTGTGCAGCCTGGAAAAAGGCGCTTTTTGTGCTACAACATGCTTGGAGCTATAACTACGATTGAATACGATGGGTACTCCCATATTGAGATAGATTTTCATGATACAAGCCGAGGTCCACGAGTTCCATCAATGACAGACCATTTTGGTTTTACAATGGCTTCACTGAATGAGAATGGAAGTGTTTTTGCAAATCCCTGCAAGGGTGAAAAGAACATGAGCACTCTCATGTATCGCCCATTTAGTAGCTGGGCAAACAATAGCGAGTGGTCTATGCGATTGGAAGGGGAAGAAGTGAAGGTTGTGGCACTTGGTACAACTTGGGTGGCAGCAATTACTAGTCTTAACTTGATCCGCATCTTCACCATTGGTGGTTTGCAGAGACATGTTCTCTCCCTCGATGGACCTGTGGTGACTGCATCAGGCTTCATGGATGAGCTTGCCATTGTCACTCATGCTTCAGACAGTCTTCCCTCAAATGAACAGGTGCTTGAATTTAGGGTACTTAACATAAATAATAGTAGCCAGCCTCTTAGAGGACGTCTGCCTCTGACTCCTGGTTCAAGTTTAACATGGTTTGGCTTTAGTGAAGAAGGGAAATTAAGCTCCTATGACTCCAAGGGTGTGCTGAGAGTTTTTACAAGTGAATATGGTGGCAGTTGGTTCCCGCTCTTCAGTGCTAGTAAAGACAAGAAGTCAGATGAAAACTATTGGGTGATCGGTTTGAATGCAAGACAACTATTTTGTATAGTTTGCAAAAGCCCCGAGTTATATCCACAGGTGTTGCCCAAACCAGTGCTCACTCTACTAAATTTTTCAATTCCTCTTGCATCCTCTGATCTAGGAGCAGAAGCCCTTGAAAATGCATTTATACTAAACGACATGCATCTCGTACAGATTCAAAAGCAAATAGAAGAAATGGATGCTGTAGGTTTAGACACCAGTTCACTTGATGATGAAGCTTTTAAATTGGAAACAGCTCAAGATCAATGTATATTGAGGCTTATTGGATCCTGCTGCCATGGAGATAAGCTTGTGAGGGCTACTGAACTTGTGAAGCATCTATCCCTGGAAAAATCAGTTAGGGGTGCAATTAAGATTGTTACTTCTCTGAAGCTACCTAACTTGGCTGAAAGGTTCAATAGCATATTGGAGGAAAGGTTGCTCAACGATAGTAAAGAACCATTGCAAACAACTGTCCCAAACCCAAACTACAATGCATCAACCTCTACCGAGAAAAGTAAGTTATCAGGGAATGATATTCCATTACCAGCACCAAAATTGTCAGCTCCTTCGTTTCTAAAGAAAGCCAAAACACAAGAAGCTAATGTTGGAACAGCGAAAACACAAGAAAAACAAACTGATCTTGAGAAGTCGACGACGACTGAGAGGGAAACTGCTGGTGCAGAGGTGAATAATGCAGCAGGTGACATGAAGAAGGTAGGGGAAATGCCCAAGGCACAACAGTCACCTCGCCCATCTAATCCATTTCTAAAGCGATCAAACAGTCAAGACATAGAGAAGAAGGAAGAAGTAGATCAGGTGAAAGCTCACCGCCCATCGAATCCGTTCTTCAAGAAATCGGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

802

Amino Acids

88.63

Weight (kDa)

5.54

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 2 - 145 5.5e-35 WDHD1 first WD40 domain
WD40_Prp19 PF24814 3 - 136 3.9e-13 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 7 - 137 2.4e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 133 2.7e-12 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 9 - 148 1.4e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_Aladin PF25460 36 - 110 9.2e-07 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 37 - 107 9.9e-08 WDR19 first beta-propeller
Beta-prop_IFT122_1st PF23381 38 - 148 1.6e-07 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 39 - 137 7e-15 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 39 - 111 4e-07 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 40 - 136 4.1e-12 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 41 - 134 2.1e-13 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 58 - 134 5.8e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 64 - 128 3e-06 TEP-1 second beta-propeller
WD40 PF00400 70 - 106 2.2e-08 WD domain, G-beta repeat
Mcl1_mid PF12341 265 - 548 3.4e-95 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 558 - 660 2.1e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2231
AatII GACGTC 1 cut(s) 1360
Acc36I ACCTGC 3 cut(s) 292, 414, 2231
AccBSI CCGCTC 1 cut(s) 1486
AccI GTMKAC 2 cut(s) 505, 2192
AciI CCGC 3 cut(s) 1171, 1484, 2371
AclWI GGATC 3 cut(s) 1162, 1821, 1834
AcsI RAATTY 3 cut(s) 350, 1310, 1627
AcuI CTGAAG 4 cut(s) 495, 1260, 1474, 1949
AcyI GRCGYC 1 cut(s) 1357
AfaI GTAC 6 cut(s) 120, 550, 893, 1132, 1320, 1710
AfiI CCNNNNNNNGG 4 cut(s) 92, 255, 1019, 1352
AflIII ACRYGT 1 cut(s) 1201
AhdI GACNNNNNGTC 1 cut(s) 1510
AhlI ACTAGT 1 cut(s) 1154
AjiI CACGTC 1 cut(s) 713
AjnI CCWGG 5 cut(s) 776, 805, 826, 1372, 1884
AjuI GAANNNNNNNTTGG 2 cut(s) 2130, 2162
AleI CACNNNNGTG 1 cut(s) 818
Alw21I GWGCWC 2 cut(s) 1040, 1617
Alw26I GTCTC 1 cut(s) 1192
AlwI GGATC 3 cut(s) 1162, 1821, 1834
Ama87I CYCGRG 1 cut(s) 1580
AoxI GGCC 2 cut(s) 779, 810
ApeKI GCWGC 4 cut(s) 823, 1145, 1833, 2237
ApoI RAATTY 3 cut(s) 350, 1310, 1627
ArsI GACNNNNNNTTYG 2 cut(s) 1542, 1574
Asp700I GAANNNNTTC 2 cut(s) 480, 2384
AspLEI GCGC 1 cut(s) 840
AspS9I GGNCC 3 cut(s) 810, 932, 1220
AsuC2I CCSGG 1 cut(s) 159
AvaI CYCGRG 1 cut(s) 1580
AvaII GGWCC 2 cut(s) 932, 1220
BamHI GGATCC 1 cut(s) 1826
BauI CACGAG 1 cut(s) 936
BbsI GAAGAC 3 cut(s) 528, 534, 1277
Bbv12I GWGCWC 2 cut(s) 1040, 1617
BbvI GCAGC 4 cut(s) 835, 1157, 1820, 2249
BccI CCATC 7 cut(s) 17, 131, 881, 952, 1211, 2302, 2383
BciT130I CCWGG 5 cut(s) 778, 807, 828, 1374, 1886
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 1192
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 5 cut(s) 401, 606, 1155, 1497, 1658
BfmI CTRYAG 2 cut(s) 27, 1743
BfoI RGCGCY 1 cut(s) 841
BfuAI ACCTGC 3 cut(s) 292, 414, 2231
BisI GCNGC 4 cut(s) 824, 1146, 1834, 2238
BlsI GCNGC 4 cut(s) 825, 1147, 1835, 2239
Bme1390I CCNGG 6 cut(s) 159, 778, 807, 828, 1374, 1886
Bme18I GGWCC 2 cut(s) 932, 1220
BmeRI GACNNNNNGTC 1 cut(s) 1510
BmeT110I CYCGRG 1 cut(s) 1580
BmgBI CACGTC 1 cut(s) 713
BmgT120I GGNCC 3 cut(s) 810, 932, 1220
BmiI GGNNCC 3 cut(s) 811, 1481, 1828
BmrFI CCNGG 6 cut(s) 159, 778, 807, 828, 1374, 1886
BmrI ACTGGG 1 cut(s) 30
BmsI GCATC 8 cut(s) 14, 1182, 1244, 1655, 1711, 1729, 1885, 2045
BmuI ACTGGG 1 cut(s) 30
BoxI GACNNNNGTC 2 cut(s) 534, 675
BpiI GAAGAC 3 cut(s) 528, 534, 1277
BpmI CTGGAG 1 cut(s) 148
BpuEI CTTGAG 2 cut(s) 1782, 2204
BpuMI CCSGG 1 cut(s) 159
Bsa29I ATCGAT 1 cut(s) 12
BsaBI GATNNNNATC 2 cut(s) 321, 1172
BsaHI GRCGYC 1 cut(s) 1357
BsaJI CCNNGG 7 cut(s) 249, 806, 928, 1434, 1837, 1884, 2271
BsaXI ACNNNNNCTCC 2 cut(s) 1961, 1991
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 255, 1019, 1352
Bse1I ACTGG 3 cut(s) 25, 1610, 1758
Bse3DI GCAATG 2 cut(s) 59, 2000
Bse8I GATNNNNATC 2 cut(s) 321, 1172
BseBI CCWGG 5 cut(s) 778, 807, 828, 1374, 1886
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 7 cut(s) 249, 806, 928, 1434, 1837, 1884, 2271
BseGI GGATG 7 cut(s) 5, 150, 322, 664, 1255, 1646, 1744
BseJI GATNNNNATC 2 cut(s) 321, 1172
BseLI CCNNNNNNNGG 4 cut(s) 92, 255, 1019, 1352
BseMI GCAATG 2 cut(s) 59, 2000
BseMII CTCAG 5 cut(s) 113, 192, 632, 1436, 2193
BseNI ACTGG 3 cut(s) 25, 1610, 1758
BseRI GAGGAG 1 cut(s) 605
BseXI GCAGC 4 cut(s) 835, 1157, 1820, 2249
BseYI CCCAGC 1 cut(s) 1065
BsgI GTGCAG 2 cut(s) 842, 2241
BshFI GGCC 2 cut(s) 781, 812
BshVI ATCGAT 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 1040, 1617
BsiHKCI CYCGRG 1 cut(s) 1580
BsiSI CCGG 2 cut(s) 158, 610
BslFI GGGAC 3 cut(s) 37, 667, 2000
BslI CCNNNNNNNGG 4 cut(s) 92, 255, 1019, 1352
BsmAI GTCTC 1 cut(s) 1192
BsmFI GGGAC 3 cut(s) 37, 667, 2000
BsmI GAATGC 1 cut(s) 1549
BsnI GGCC 2 cut(s) 781, 812
BsoBI CYCGRG 1 cut(s) 1580
Bsp1286I GDGCHC 2 cut(s) 1040, 1617
Bsp19I CCATGG 1 cut(s) 1837
BspACI CCGC 3 cut(s) 1171, 1484, 2371
BspANI GGCC 2 cut(s) 781, 812
BspCNI CTCAG 5 cut(s) 114, 193, 633, 1437, 2194
BspDI ATCGAT 1 cut(s) 12
BspHI TCATGA 1 cut(s) 916
BspLI GGNNCC 3 cut(s) 811, 1481, 1828
BspMI ACCTGC 3 cut(s) 292, 414, 2231
BspPI GGATC 3 cut(s) 1162, 1821, 1834
BspQI GCTCTTC 1 cut(s) 1493
BsrBI CCGCTC 1 cut(s) 1486
BsrDI GCAATG 2 cut(s) 59, 2000
BsrI ACTGG 3 cut(s) 25, 1610, 1758
BssECI CCNNGG 7 cut(s) 249, 806, 928, 1434, 1837, 1884, 2271
BssNI GRCGYC 1 cut(s) 1357
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 4 cut(s) 249, 1434, 1837, 2271
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 5 cut(s) 778, 807, 828, 1374, 1886
Bst4CI ACNGT 7 cut(s) 28, 237, 768, 1283, 2014, 2283, 2324
Bst6I CTCTTC 1 cut(s) 1493
BstACI GRCGYC 1 cut(s) 1357
BstC8I GCNNGC 3 cut(s) 626, 1257, 1343
BstDEI CTNAG 8 cut(s) 122, 201, 597, 620, 641, 1349, 1445, 2202
BstDSI CCRYGG 1 cut(s) 1837
BstEII GGTNACC 1 cut(s) 258
BstENI CCTNNNNNAGG 1 cut(s) 1350
BstF5I GGATG 7 cut(s) 5, 150, 322, 664, 1255, 1646, 1744
BstH2I RGCGCY 1 cut(s) 841
BstHHI GCGC 1 cut(s) 840
BstMAI GTCTC 1 cut(s) 1192
BstMWI GCNNNNNNNGC 2 cut(s) 787, 846
BstNI CCWGG 5 cut(s) 778, 807, 828, 1374, 1886
BstNSI RCATGY 3 cut(s) 859, 1205, 1702
BstPAI GACNNNNGTC 2 cut(s) 534, 675
BstPI GGTNACC 1 cut(s) 258
BstSCI CCNGG 6 cut(s) 157, 776, 805, 826, 1372, 1884
BstSFI CTRYAG 2 cut(s) 27, 1743
BstV1I GCAGC 4 cut(s) 835, 1157, 1820, 2249
BstV2I GAAGAC 3 cut(s) 528, 534, 1277
BstX2I RGATCY 1 cut(s) 1826
BstYI RGATCY 1 cut(s) 1826
Bsu15I ATCGAT 1 cut(s) 12
BsuRI GGCC 2 cut(s) 781, 812
BsuTUI ATCGAT 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 1837
BtrI CACGTC 1 cut(s) 713
BtsCI GGATG 7 cut(s) 5, 150, 322, 664, 1255, 1646, 1744
BtsI GCAGTG 2 cut(s) 195, 450
BtsIMutI CAGTG 7 cut(s) 195, 450, 471, 732, 980, 1498, 1617
BveI ACCTGC 3 cut(s) 292, 414, 2231
Cac8I GCNNGC 3 cut(s) 626, 1257, 1343
CciI TCATGA 1 cut(s) 916
CfoI GCGC 1 cut(s) 840
Cfr13I GGNCC 3 cut(s) 810, 932, 1220
ClaI ATCGAT 1 cut(s) 12
Csp6I GTAC 6 cut(s) 119, 549, 892, 1131, 1319, 1709
CspCI CAANNNNNGTGG 4 cut(s) 1122, 1157, 1581, 1616
CviQI GTAC 6 cut(s) 119, 549, 892, 1131, 1319, 1709
DdeI CTNAG 8 cut(s) 122, 201, 597, 620, 641, 1349, 1445, 2202
DraI TTTAAA 2 cut(s) 651, 1783
DriI GACNNNNNGTC 1 cut(s) 1510
Eam1104I CTCTTC 1 cut(s) 1493
Eam1105I GACNNNNNGTC 1 cut(s) 1510
EarI CTCTTC 1 cut(s) 1493
Eco130I CCWWGG 4 cut(s) 249, 1434, 1837, 2271
Eco47I GGWCC 2 cut(s) 932, 1220
Eco57I CTGAAG 4 cut(s) 495, 1260, 1474, 1949
Eco88I CYCGRG 1 cut(s) 1580
Eco91I GGTNACC 1 cut(s) 258
EcoNI CCTNNNNNAGG 1 cut(s) 1350
EcoO65I GGTNACC 1 cut(s) 258
EcoRII CCWGG 5 cut(s) 776, 805, 826, 1372, 1884
EcoT14I CCWWGG 4 cut(s) 249, 1434, 1837, 2271
EcoT22I ATGCAT 3 cut(s) 1684, 1704, 2038
ErhI CCWWGG 4 cut(s) 249, 1434, 1837, 2271
FaqI GGGAC 3 cut(s) 37, 667, 2000
FauI CCCGC 1 cut(s) 1491
FauNDI CATATG 1 cut(s) 586
FblI GTMKAC 2 cut(s) 505, 2192
Fnu4HI GCNGC 4 cut(s) 824, 1146, 1834, 2238
FokI GGATG 6 cut(s) 157, 329, 671, 1262, 1633, 1751
Fsp4HI GCNGC 4 cut(s) 824, 1146, 1834, 2238
FspBI CTAG 5 cut(s) 401, 606, 1155, 1497, 1658
GlaI GCGC 1 cut(s) 839
GluI GCNGC 4 cut(s) 824, 1146, 1834, 2238
GsaI CCCAGC 1 cut(s) 1069
GsuI CTGGAG 1 cut(s) 148
HaeII RGCGCY 1 cut(s) 841
HaeIII GGCC 2 cut(s) 781, 812
HapII CCGG 2 cut(s) 158, 610
HhaI GCGC 1 cut(s) 840
Hin1I GRCGYC 1 cut(s) 1357
Hin6I GCGC 1 cut(s) 838
HinP1I GCGC 1 cut(s) 838
HincII GTYRAC 1 cut(s) 2193
HindII GTYRAC 1 cut(s) 2193
HindIII AAGCTT 2 cut(s) 1776, 1847
HpaII CCGG 2 cut(s) 158, 610
Hpy166II GTNNAC 5 cut(s) 227, 506, 935, 1763, 2193
Hpy188III TCNNGA 8 cut(s) 51, 134, 539, 917, 1799, 2183, 2327, 2392
Hpy8I GTNNAC 5 cut(s) 227, 506, 935, 1763, 2193
Hpy99I CGWCG 3 cut(s) 717, 2197, 2200
HpyAV CCTTC 7 cut(s) 462, 1094, 1108, 1403, 2118, 2248, 2335
HpyCH4III ACNGT 7 cut(s) 28, 237, 768, 1283, 2014, 2283, 2324
HpyCH4IV ACGT 2 cut(s) 712, 1357
HpyF10VI GCNNNNNNNGC 2 cut(s) 787, 846
HpyF3I CTNAG 8 cut(s) 122, 201, 597, 620, 641, 1349, 1445, 2202
HpySE526I ACGT 2 cut(s) 712, 1357
Hsp92I GRCGYC 1 cut(s) 1357
HspAI GCGC 1 cut(s) 838
LguI GCTCTTC 1 cut(s) 1493
LmnI GCTCC 5 cut(s) 88, 863, 1427, 1661, 2110
Lsp1109I GCAGC 4 cut(s) 835, 1157, 1820, 2249
LweI GCATC 8 cut(s) 14, 1182, 1244, 1655, 1711, 1729, 1885, 2045
MaeI CTAG 5 cut(s) 401, 606, 1155, 1497, 1658
MaeII ACGT 2 cut(s) 712, 1357
MaeIII GTNAC 6 cut(s) 258, 1228, 1264, 1918, 2243, 2283
MbiI CCGCTC 1 cut(s) 1486
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 1 cut(s) 1826
MhlI GDGCHC 2 cut(s) 1040, 1617
MlyI GAGTC 5 cut(s) 139, 602, 629, 1363, 1424
MmeI TCCRAC 2 cut(s) 695, 2128
Mph1103I ATGCAT 3 cut(s) 1684, 1704, 2038
MroXI GAANNNNTTC 2 cut(s) 480, 2384
MslI CAYNNNNRTG 2 cut(s) 818, 1043
MspI CCGG 2 cut(s) 158, 610
MspR9I CCNGG 6 cut(s) 159, 778, 807, 828, 1374, 1886
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 1549
MvaI CCWGG 5 cut(s) 778, 807, 828, 1374, 1886
MwoI GCNNNNNNNGC 2 cut(s) 787, 846
NciI CCSGG 1 cut(s) 159
NcoI CCATGG 1 cut(s) 1837
NdeI CATATG 1 cut(s) 586
NlaIV GGNNCC 3 cut(s) 811, 1481, 1828
NmeAIII GCCGAG 1 cut(s) 953
NmuCI GTSAC 5 cut(s) 258, 1228, 1264, 2243, 2283
NsiI ATGCAT 3 cut(s) 1684, 1704, 2038
NspI RCATGY 3 cut(s) 859, 1205, 1702
OliI CACNNNNGTG 1 cut(s) 818
PagI TCATGA 1 cut(s) 916
PaqCI CACCTGC 1 cut(s) 2231
PciI ACATGT 1 cut(s) 1201
PciSI GCTCTTC 1 cut(s) 1493
PctI GAATGC 1 cut(s) 1549
PdmI GAANNNNTTC 2 cut(s) 480, 2384
PfeI GAWTC 7 cut(s) 14, 448, 487, 695, 718, 1714, 2380
PflFI GACNNNGTC 1 cut(s) 395
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 4 cut(s) 825, 1147, 1835, 2239
PleI GAGTC 5 cut(s) 138, 601, 629, 1363, 1424
PpsI GAGTC 5 cut(s) 138, 601, 629, 1363, 1424
PscI ACATGT 1 cut(s) 1201
PshAI GACNNNNGTC 2 cut(s) 534, 675
Psp6I CCWGG 5 cut(s) 776, 805, 826, 1372, 1884
PspEI GGTNACC 1 cut(s) 258
PspFI CCCAGC 1 cut(s) 1065
PspGI CCWGG 5 cut(s) 776, 805, 826, 1372, 1884
PspN4I GGNNCC 3 cut(s) 811, 1481, 1828
PspPI GGNCC 3 cut(s) 810, 932, 1220
PsuI RGATCY 1 cut(s) 1826
PsyI GACNNNGTC 1 cut(s) 395
RsaI GTAC 6 cut(s) 120, 550, 893, 1132, 1320, 1710
RsaNI GTAC 6 cut(s) 119, 549, 892, 1131, 1319, 1709
RseI CAYNNNNRTG 2 cut(s) 818, 1043
SalI GTCGAC 1 cut(s) 2191
SapI GCTCTTC 1 cut(s) 1493
SatI GCNGC 4 cut(s) 824, 1146, 1834, 2238
Sau96I GGNCC 3 cut(s) 810, 932, 1220
SchI GAGTC 5 cut(s) 139, 602, 629, 1363, 1424
ScrFI CCNGG 6 cut(s) 159, 778, 807, 828, 1374, 1886
SduI GDGCHC 2 cut(s) 1040, 1617
SfaNI GCATC 8 cut(s) 14, 1182, 1244, 1655, 1711, 1729, 1885, 2045
SfcI CTRYAG 2 cut(s) 27, 1743
SinI GGWCC 2 cut(s) 932, 1220
SmiI ATTTAAAT 1 cut(s) 651
SmiMI CAYNNNNRTG 2 cut(s) 818, 1043
SmlI CTYRAG 2 cut(s) 1797, 2183
SmoI CTYRAG 2 cut(s) 1797, 2183
SpeI ACTAGT 1 cut(s) 1154
SsiI CCGC 3 cut(s) 1171, 1484, 2371
SspMI CTAG 5 cut(s) 401, 606, 1155, 1497, 1658
StyD4I CCNGG 6 cut(s) 157, 776, 805, 826, 1372, 1884
StyI CCWWGG 4 cut(s) 249, 1434, 1837, 2271
SwaI ATTTAAAT 1 cut(s) 651
TaaI ACNGT 7 cut(s) 28, 237, 768, 1283, 2014, 2283, 2324
TaiI ACGT 2 cut(s) 715, 1360
TaqI TCGA 4 cut(s) 12, 1215, 2192, 2378
TaqII GACCGA 1 cut(s) 2389
TatI WGTACW 2 cut(s) 118, 548
TfiI GAWTC 7 cut(s) 14, 448, 487, 695, 718, 1714, 2380
TscAI CASTG 7 cut(s) 202, 450, 478, 739, 987, 1498, 1617
TseFI GTSAC 5 cut(s) 258, 1228, 1264, 2243, 2283
TseI GCWGC 4 cut(s) 823, 1145, 1833, 2237
Tsp45I GTSAC 5 cut(s) 258, 1228, 1264, 2243, 2283
TspDTI ATGAA 9 cut(s) 444, 476, 534, 905, 1234, 1311, 1533, 1788, 2264
TspGWI ACGGA 2 cut(s) 157, 2373
TspRI CASTG 7 cut(s) 202, 450, 478, 739, 987, 1498, 1617
Tth111I GACNNNGTC 1 cut(s) 395
VpaK11BI GGWCC 2 cut(s) 932, 1220
XagI CCTNNNNNAGG 1 cut(s) 1350
XapI RAATTY 3 cut(s) 350, 1310, 1627
XceI RCATGY 3 cut(s) 859, 1205, 1702
XcmI CCANNNNNNNNNTGG 1 cut(s) 1062
XmiI GTMKAC 2 cut(s) 505, 2192
XmnI GAANNNNTTC 2 cut(s) 480, 2384
XspI CTAG 5 cut(s) 401, 606, 1155, 1497, 1658
ZraI GACGTC 1 cut(s) 1358
Zsp2I ATGCAT 3 cut(s) 1684, 1704, 2038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.