MD06G1206600.v1.1

WD repeat and HMG-box DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005318
Physical Location & Seq
Forward (+)
34147437 .. 34152307
4871 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1206600.v1.1.491

Sequence Viewer

Length: 2883 bp
ATGAAGATCCGAACGTTGAAGCTGCGAGAGGCCCACAAGGCCACGAGCAGCGGCGGCGGGCCGTCGTACTGCTCGGTGTTGTGGGACCAGAAAGCCTACCACGTGGTGACGGGCTCCTCCTCCGACCCCACAATCGCTATCCACGACTCTCTTCTGCTGTCCAGCCCTCCGAAGCTCCTCCGCCACCACCGCGACGGTGTCACGGCTCTCGCTCTCAGCCCCAACTCCACCTGCCTCGCCTCCGGCTCCGTTGACCACTCCGTCAAGCTCTACAAGTTTCCAGGTGGAGAATTTCAGACTAATATAACCCGATTCACCCTGCCAATACGCGCCCTGGCGTTTAACAAATCCGGCACGCTATTAGCAGCTGCTGGTGATGATGAAGGCGTTAAGCTTATTAACACAGTTGATGGTTCAATTTCAAGAGTTCTCAAAGGACACAAAGGACCTGTGACCGGCTTGGGTTTCGATCCCCTCAGCGAATACTTGGCCTCTATTGATTCCACTGGGACTGTTATATACTGGGAACTTTCATCTGGGACTACGTTGCATACCCTTAAAGGGGTAGCTCCTAACTCGGGTTTTGATCACTCCATCATGAATGTACTTAGCTGGAGTCCTGATGGTGACAAGTTAGCCGTGCCAGGGTTGAGAAATGATGTGGTGGTTTATGATAGAGACACAGCTGAAAAGCTATTTTCGTTGAGAGGTGATCACACGCAGCCTATTTGTTCCTTGGCTTGGTCGCCTAACGGAAAATACATGGCTACTTCTGGTTTGGATAAGCAGGTTCTCATATGGGATGTTGATCGGAAGCAGGACATTGACAGGCAGAACTTTGATGAGAGAATATGTTGCTTGGCGTGGAAGCCGATTGGCAATGCACTGGCTGTTATTGATTGTATGGGCAAGTATGGTGTGTGGGATTCGGTGACTCCTTCATCAATGAAGTCTCCCACTGAAGATGTTCCCAATCTACAGTCTAAGAACAGCAATGGGCTACTTTTGTTTGACGAAGAAGAGGGTCAGGAGCTTAGCACTTCTGGTAGTTTAAGTGATGTTGGTGAAGATAGTATTGGGGAATCCAAGCCACCTAGCAGGAAGAGGCCGCACAAGCACACTGCATATGAGAATGATTTGGAAGAGGATGGTTTTGATGGCTTTAGGTTGATACCAAATGTTGAGTCGGAGTCCCGTAAAAAAGCACGGCGTAAACACAGTGAAAATGCTGATAATGAGAATGAGGGAGTAAGCAGCAAAATGACATCAATTAGATCCAAAATGCAGGTGTCATTCCAGCCTGGTGCTACTCCTTTGCAGCCAGGAAAAAGGCGCTTTCTGTGCTATAACATGCTTGGCTCCATAACTACAATTGAACATGATGGGTTCTCCCATATTGAGATAGATTTTCATGATACAAGTCGAGGCCCAAGAGTTCCATCAATGAATGATCATTTTGGTTTTACAATGGCTTCACTTAATGAGAATGGAAGCGTTTTTGCAAATCCGTGCAAGGGCGAAAAGCACATGAGTACTCTCATGTATCGCCCATTCAGTAGCTGGGCGAATAATAGTGAGTGGTCTATGCGATTGGAAGGGGAAGAAGTGAAGGTTGTGGCACTTGGTACAGCTTGGGTGGCAGCAATTACGAGTCTTAACTTCCTTCGCATTTTCACTGACAGTGGTTTGCAGAAACATGTTCTCTCCCTTGATGGACCTGTCGTGACTGCATCAGGCTTCAAGGATGAGCTTGCGATTGTCACTCATGCTTCTGATTGTCTCCCCTCAAATGAGCAGATGCTTGAATTTAGAGTGCTCAACATAACTAAGGGGAGCGAGCCTCTTAGAGGACGGCTGCCATTGACTCCTGGTTCACATTTAACATGGTTTGGATTTAGTGAAGAAGGGCAATTAAGCTCCTATGATTCCAAAGGAGCGATGAGAGTTTTTACAAGCCAATATGGTGGCAGTTGGCTCCCACTCTTCAGTGCTAGCAAAGAGAAGAAGTCAAACGAAAACTATTGGGTGGTTGGGTTGAATGCAAGCAAATTGTTTTGTATAGTTTGCAAAAGTCCTGACTTATACCCGCAAGTGATGCCGAAGCCAGTCCTCACTCCGCTAAATTTTTCATTCCCTCTAGCTTCCTCTGATCTAGGTGCAGAAGCCCTTGAAAATGAGTTTATACTAAACAACACGCACCTTGTACAGATTCAAAAGCAAATAGAAGAAGTGGGCGCTGCTGGTTTGGACACCACTTCACTTGACGATGAGGCTTTCAATACAGAAGCAGCTCAAGACAGATGCATCTTAAAGCTTATTGCGTCCTGCTGCAACGGAGATAAGCTTGTGAGAGCGATTGAACTCGTGAAGCTTCTGTCCCTCGAAAAATCAGTAAGGGGTGCAATTAAACTTGTCACCGTTCTGAAGCTCCCTAACTTGGCTGAAAGGTTCAACAGCATACTGGAGGAAAGGTTGCTCAACGAGAGTAAAAGAACCGCGGAAACCACTTTCCCTTCCTCAAACTGCACCGCATCTTTCATCACGGATGACATTGCACCCATAAAGAAAATGATCTCTACCGAGCAAAGTAAGTTGTCAGGAAACGGCATTCCATTATCAACACCAAAACTGTCTGCTCCCTTGTTTACCAAGAAAGCGAAAACGCAAGAACCTAAAGTTGGAACAGAGAAAACAGACGAAAAACAAACTGCGACGGCAGATGATGATGTTGGGAAGGTAAAAACGACAGAGGTGAGCAACAATGCAGGAGAGATGAAGAAGGTAGGGGAAGTGTCTGAGGTATCAAATGATCAAGAAAAAGTGAAGAAGGCAGAAGCACCCCGTCGAGCATCTAATCCATTCATGAAGAAATTAATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

961

Amino Acids

105.08

Weight (kDa)

6.31

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 22 - 138 2e-13 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 24 - 139 4e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 25 - 307 4.6e-71 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 34 - 187 2.8e-22 WDR5 beta-propeller domain
WD40 PF00400 58 - 91 5.9e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 59 - 188 3.2e-18 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 62 - 273 2.8e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 214 1.7e-10 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 64 - 187 4.6e-08 WDR55
WD40_Prp19 PF24814 64 - 299 6e-32 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 64 - 188 7.5e-09 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 107 - 184 1e-05 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR19_1st PF23389 116 - 251 2.6e-09 WDR19 first beta-propeller
WD40_CDC20-Fz PF24807 128 - 291 2.1e-14 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 145 - 277 4.4e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 168 - 273 1e-05 WDR36/Utp21 first beta-propeller
Beta-prop_IFT122_1st PF23381 199 - 313 1.6e-07 IFT122 first beta-propeller
Beta-prop_WDR3_1st PF25173 200 - 276 5.6e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 200 - 318 8.6e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 201 - 315 3.2e-11 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 203 - 277 6e-06 CAF1B/HIR1 beta-propeller domain
ANAPC4_WD40 PF12894 203 - 290 2.2e-07 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 211 - 300 4.4e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_Aladin PF25460 226 - 309 1.8e-07 Aladin seven-bladed propeller
WD40 PF00400 238 - 268 7.9e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 246 - 310 9e-06 Echinoderm microtubule-associated protein second beta-propeller
Mcl1_mid PF12341 431 - 713 8.1e-94 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 722 - 825 1.8e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 239, 1276
AasI GACNNNNNNGTC 1 cut(s) 260
Acc36I ACCTGC 3 cut(s) 239, 778, 1276
AccII CGCG 3 cut(s) 192, 330, 2498
AclI AACGTT 1 cut(s) 14
AclWI GGATC 2 cut(s) 464, 1269
AcsI RAATTY 3 cut(s) 290, 1805, 2122
AcuI CTGAAG 3 cut(s) 981, 1969, 2444
AcvI CACGTG 1 cut(s) 103
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 5 cut(s) 68, 606, 1534, 1627, 2205
AflIII ACRYGT 1 cut(s) 1696
AjnI CCWGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
AjuI GAANNNNNNNTTGG 4 cut(s) 1059, 1091, 2661, 2693
Alw21I GWGCWC 1 cut(s) 1818
Alw26I GTCTC 3 cut(s) 672, 957, 1784
AlwI GGATC 2 cut(s) 464, 1269
AlwNI CAGNNNCTG 2 cut(s) 371, 1560
Ama87I CYCGRG 1 cut(s) 577
AoxI GGCC 6 cut(s) 30, 39, 59, 489, 1106, 1426
ApoI RAATTY 3 cut(s) 290, 1805, 2122
ArsI GACNNNNNNTTYG 2 cut(s) 1169, 1201
AseI ATTAAT 1 cut(s) 2873
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 3 cut(s) 332, 1335, 2237
AspS9I GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
AsuHPI GGTGA 9 cut(s) 118, 307, 386, 638, 722, 943, 1076, 2407, 2764
AsuNHI GCTAGC 1 cut(s) 1991
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 3 cut(s) 85, 446, 1715
BanII GRGCYC 1 cut(s) 116
BauI CACGAG 2 cut(s) 43, 2363
BbrPI CACGTG 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 1818
BbvCI CCTCAGC 1 cut(s) 476
BccI CCATC 8 cut(s) 404, 602, 617, 1142, 1151, 1376, 1447, 1706
BceAI ACGGC 7 cut(s) 46, 219, 623, 1223, 1868, 2620, 2730
BciT130I CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
BclI TGATCA 4 cut(s) 586, 712, 1450, 2809
BcoDI GTCTC 3 cut(s) 672, 957, 1784
BfaI CTAG 4 cut(s) 1095, 1992, 2138, 2153
BfmI CTRYAG 1 cut(s) 977
BfoI RGCGCY 2 cut(s) 1336, 2238
BfuAI ACCTGC 3 cut(s) 239, 778, 1276
BglI GCCNNNNNGGC 1 cut(s) 38
BlpI GCTNAGC 1 cut(s) 1034
BmcAI AGTACT 1 cut(s) 1534
Bme1390I CCNGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
Bme18I GGWCC 3 cut(s) 85, 446, 1715
BmeT110I CYCGRG 1 cut(s) 577
BmgT120I GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
BmiI GGNNCC 5 cut(s) 86, 115, 247, 1360, 1976
BmrFI CCNGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
BmrI ACTGGG 2 cut(s) 516, 532
BmsI GCATC 7 cut(s) 1739, 1788, 2085, 2291, 2313, 2540, 2858
BmtI GCTAGC 1 cut(s) 1995
BmuI ACTGGG 2 cut(s) 516, 532
BplI GAGNNNNNCTC 2 cut(s) 1825, 1857
BpmI CTGGAG 2 cut(s) 634, 2483
Bpu10I CCTNAGC 1 cut(s) 476
Bpu1102I GCTNAGC 1 cut(s) 1034
BpuEI CTTGAG 1 cut(s) 2277
BsaAI YACGTR 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 807
BsaJI CCNNGG 4 cut(s) 333, 644, 735, 2496
BsaXI ACNNNNNCTCC 2 cut(s) 2456, 2486
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 5 cut(s) 511, 527, 891, 2105, 2466
Bse3DI GCAATG 3 cut(s) 886, 1000, 2550
Bse8I GATNNNNATC 1 cut(s) 807
BseBI CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
BseDI CCNNGG 4 cut(s) 333, 644, 735, 2496
BseGI GGATG 4 cut(s) 808, 1153, 1750, 2551
BseJI GATNNNNATC 1 cut(s) 807
BseMI GCAATG 3 cut(s) 886, 1000, 2550
BseMII CTCAG 3 cut(s) 229, 490, 2787
BseNI ACTGG 5 cut(s) 511, 527, 891, 2105, 2466
BseRI GAGGAG 3 cut(s) 106, 109, 167
BseYI CCCAGC 1 cut(s) 1560
BsgI GTGCAG 2 cut(s) 2178, 2509
Bsh1236I CGCG 3 cut(s) 192, 330, 2498
BshFI GGCC 6 cut(s) 32, 41, 61, 491, 1108, 1428
BsiHKAI GWGCWC 1 cut(s) 1818
BsiHKCI CYCGRG 1 cut(s) 577
BsiSI CCGG 3 cut(s) 243, 351, 456
BslFI GGGAC 5 cut(s) 98, 523, 553, 1177, 2362
BsmAI GTCTC 3 cut(s) 672, 957, 1784
BsmFI GGGAC 5 cut(s) 98, 523, 553, 1177, 2362
BsmI GAATGC 2 cut(s) 2044, 2607
BsnI GGCC 6 cut(s) 32, 41, 61, 491, 1108, 1428
BsoBI CYCGRG 1 cut(s) 577
Bsp1286I GDGCHC 2 cut(s) 116, 1818
Bsp1407I TGTACA 1 cut(s) 2203
Bsp1720I GCTNAGC 1 cut(s) 1034
BspANI GGCC 6 cut(s) 32, 41, 61, 491, 1108, 1428
BspCNI CTCAG 3 cut(s) 228, 489, 2788
BspFNI CGCG 3 cut(s) 192, 330, 2498
BspHI TCATGA 3 cut(s) 597, 1411, 2862
BspLI GGNNCC 5 cut(s) 86, 115, 247, 1360, 1976
BspMI ACCTGC 3 cut(s) 239, 778, 1276
BspOI GCTAGC 1 cut(s) 1995
BspPI GGATC 2 cut(s) 464, 1269
BsrDI GCAATG 3 cut(s) 886, 1000, 2550
BsrFI RCCGGY 1 cut(s) 455
BsrGI TGTACA 1 cut(s) 2203
BsrI ACTGG 5 cut(s) 511, 527, 891, 2105, 2466
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 4 cut(s) 333, 644, 735, 2496
BssSI CACGAG 2 cut(s) 43, 2363
BssT1I CCWWGG 1 cut(s) 735
Bst2BI CACGAG 2 cut(s) 43, 2363
Bst2UI CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
Bst4CI ACNGT 8 cut(s) 197, 406, 514, 981, 1220, 1682, 2419, 2631
Bst6I CTCTTC 5 cut(s) 156, 1014, 1097, 1137, 1988
BstAPI GCANNNNNTGC 1 cut(s) 2095
BstAUI TGTACA 1 cut(s) 2203
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 6 cut(s) 59, 356, 1752, 1838, 1993, 2044
BstDEI CTNAG 8 cut(s) 215, 476, 608, 984, 1034, 1827, 1844, 2796
BstDSI CCRYGG 1 cut(s) 2496
BstENI CCTNNNNNAGG 1 cut(s) 1845
BstF5I GGATG 4 cut(s) 808, 1153, 1750, 2551
BstFNI CGCG 3 cut(s) 192, 330, 2498
BstH2I RGCGCY 2 cut(s) 1336, 2238
BstHHI GCGC 3 cut(s) 332, 1335, 2237
BstMAI GTCTC 3 cut(s) 672, 957, 1784
BstMWI GCNNNNNNNGC 7 cut(s) 38, 54, 189, 1114, 1341, 1637, 2095
BstNI CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
BstNSI RCATGY 2 cut(s) 1354, 1700
BstSCI CCNGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
BstSFI CTRYAG 1 cut(s) 977
BstUI CGCG 3 cut(s) 192, 330, 2498
BstX2I RGATCY 2 cut(s) 6, 1274
BstXI CCANNNNNNTGG 1 cut(s) 1964
BstYI RGATCY 2 cut(s) 6, 1274
BsuRI GGCC 6 cut(s) 32, 41, 61, 491, 1108, 1428
BtgI CCRYGG 1 cut(s) 2496
BtgZI GCGATG 1 cut(s) 1952
BtsCI GGATG 4 cut(s) 808, 1153, 1750, 2551
BtsI GCAGTG 1 cut(s) 1119
BtsIMutI CAGTG 8 cut(s) 504, 884, 957, 1119, 1225, 1674, 1687, 1993
BveI ACCTGC 3 cut(s) 239, 778, 1276
Cac8I GCNNGC 6 cut(s) 59, 356, 1752, 1838, 1993, 2044
CaiI CAGNNNCTG 2 cut(s) 371, 1560
CciI TCATGA 3 cut(s) 597, 1411, 2862
CfoI GCGC 3 cut(s) 332, 1335, 2237
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
Cfr42I CCGCGG 1 cut(s) 2499
CseI GACGC 1 cut(s) 2310
Csp6I GTAC 5 cut(s) 67, 605, 1533, 1626, 2204
CspCI CAANNNNNGTGG 2 cut(s) 2242, 2277
CviQI GTAC 5 cut(s) 67, 605, 1533, 1626, 2204
DdeI CTNAG 8 cut(s) 215, 476, 608, 984, 1034, 1827, 1844, 2796
DraIII CACNNNGTG 1 cut(s) 106
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 5 cut(s) 156, 1014, 1097, 1137, 1988
EarI CTCTTC 5 cut(s) 156, 1014, 1097, 1137, 1988
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 116
Eco47I GGWCC 3 cut(s) 85, 446, 1715
Eco57I CTGAAG 3 cut(s) 981, 1969, 2444
Eco72I CACGTG 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 1845
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
EcoT14I CCWWGG 1 cut(s) 735
EcoT22I ATGCAT 1 cut(s) 2306
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 735
FaqI GGGAC 5 cut(s) 98, 523, 553, 1177, 2362
FauI CCCGC 2 cut(s) 50, 2094
FauNDI CATATG 2 cut(s) 797, 1126
FbaI TGATCA 4 cut(s) 586, 712, 1450, 2809
FokI GGATG 4 cut(s) 815, 1160, 1757, 2558
FriOI GRGCYC 1 cut(s) 116
FspBI CTAG 4 cut(s) 1095, 1992, 2138, 2153
GlaI GCGC 3 cut(s) 331, 1334, 2236
GsaI CCCAGC 1 cut(s) 1564
GsuI CTGGAG 2 cut(s) 634, 2483
HaeII RGCGCY 2 cut(s) 1336, 2238
HaeIII GGCC 6 cut(s) 32, 41, 61, 491, 1108, 1428
HapII CCGG 3 cut(s) 243, 351, 456
HgaI GACGC 1 cut(s) 2310
HhaI GCGC 3 cut(s) 332, 1335, 2237
Hin6I GCGC 3 cut(s) 330, 1333, 2235
HinP1I GCGC 3 cut(s) 330, 1333, 2235
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 4 cut(s) 392, 2312, 2342, 2369
HpaII CCGG 3 cut(s) 243, 351, 456
HphI GGTGA 9 cut(s) 118, 307, 386, 638, 722, 943, 1076, 2407, 2764
Hpy166II GTNNAC 4 cut(s) 253, 1214, 1874, 2646
Hpy8I GTNNAC 4 cut(s) 253, 1214, 1874, 2646
Hpy99I CGWCG 4 cut(s) 67, 197, 2716, 2847
HpyCH4III ACNGT 8 cut(s) 197, 406, 514, 981, 1220, 1682, 2419, 2631
HpyCH4IV ACGT 3 cut(s) 14, 102, 545
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 54, 189, 1114, 1341, 1637, 2095
HpyF3I CTNAG 8 cut(s) 215, 476, 608, 984, 1034, 1827, 1844, 2796
HpySE526I ACGT 3 cut(s) 14, 102, 545
HspAI GCGC 3 cut(s) 330, 1333, 2235
Ksp22I TGATCA 4 cut(s) 586, 712, 1450, 2809
KspI CCGCGG 1 cut(s) 2499
LweI GCATC 7 cut(s) 1739, 1788, 2085, 2291, 2313, 2540, 2858
MaeI CTAG 4 cut(s) 1095, 1992, 2138, 2153
MaeII ACGT 3 cut(s) 14, 102, 545
MaeIII GTNAC 8 cut(s) 106, 199, 451, 626, 931, 1723, 1759, 2413
MfeI CAATTG 1 cut(s) 1371
MflI RGATCY 2 cut(s) 6, 1274
MhlI GDGCHC 2 cut(s) 116, 1818
MlyI GAGTC 7 cut(s) 140, 625, 928, 1193, 1199, 1660, 1858
MmeI TCCRAC 3 cut(s) 147, 1167, 2659
Mph1103I ATGCAT 1 cut(s) 2306
MroXI GAANNNNTTC 1 cut(s) 966
MspA1I CMGCKG 4 cut(s) 51, 368, 686, 2498
MspI CCGG 3 cut(s) 243, 351, 456
MspR9I CCNGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
MunI CAATTG 1 cut(s) 1371
Mva1269I GAATGC 2 cut(s) 2044, 2607
MvaI CCWGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
MvnI CGCG 3 cut(s) 192, 330, 2498
MwoI GCNNNNNNNGC 7 cut(s) 38, 54, 189, 1114, 1341, 1637, 2095
NdeI CATATG 2 cut(s) 797, 1126
NheI GCTAGC 1 cut(s) 1991
NlaIV GGNNCC 5 cut(s) 86, 115, 247, 1360, 1976
NmuCI GTSAC 8 cut(s) 106, 199, 451, 626, 931, 1723, 1759, 2413
NsiI ATGCAT 1 cut(s) 2306
NspI RCATGY 2 cut(s) 1354, 1700
PacI TTAATTAA 1 cut(s) 2877
PagI TCATGA 3 cut(s) 597, 1411, 2862
PaqCI CACCTGC 2 cut(s) 239, 1276
PciI ACATGT 1 cut(s) 1696
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 2 cut(s) 2044, 2607
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 6 cut(s) 312, 500, 926, 1082, 1925, 2209
PflFI GACNNNGTC 1 cut(s) 197
PleI GAGTC 7 cut(s) 140, 624, 928, 1192, 1198, 1659, 1858
PmaCI CACGTG 1 cut(s) 103
PmlI CACGTG 1 cut(s) 103
PpsI GAGTC 7 cut(s) 140, 624, 928, 1192, 1198, 1659, 1858
Ppu21I YACGTR 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 1 cut(s) 1696
PshBI ATTAAT 1 cut(s) 2873
Psp1406I AACGTT 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
PspCI CACGTG 1 cut(s) 103
PspFI CCCAGC 1 cut(s) 1560
PspGI CCWGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
PspN4I GGNNCC 5 cut(s) 86, 115, 247, 1360, 1976
PspPI GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
PspPPI RGGWCCY 1 cut(s) 446
PstNI CAGNNNCTG 2 cut(s) 371, 1560
PsuI RGATCY 2 cut(s) 6, 1274
PsyI GACNNNGTC 1 cut(s) 197
PvuII CAGCTG 2 cut(s) 368, 686
RsaI GTAC 5 cut(s) 68, 606, 1534, 1627, 2205
RsaNI GTAC 5 cut(s) 67, 605, 1533, 1626, 2204
SacII CCGCGG 1 cut(s) 2499
Sau96I GGNCC 6 cut(s) 31, 59, 85, 446, 1427, 1715
ScaI AGTACT 1 cut(s) 1534
SchI GAGTC 7 cut(s) 140, 625, 928, 1193, 1199, 1660, 1858
ScrFI CCNGG 6 cut(s) 282, 335, 645, 1302, 1323, 1869
SduI GDGCHC 2 cut(s) 116, 1818
SfaNI GCATC 7 cut(s) 1739, 1788, 2085, 2291, 2313, 2540, 2858
SfcI CTRYAG 1 cut(s) 977
SfiI GGCCNNNNNGGCC 1 cut(s) 38
Sfr303I CCGCGG 1 cut(s) 2499
SgrBI CCGCGG 1 cut(s) 2499
SinI GGWCC 3 cut(s) 85, 446, 1715
SmlI CTYRAG 1 cut(s) 2292
SmoI CTYRAG 1 cut(s) 2292
SspMI CTAG 4 cut(s) 1095, 1992, 2138, 2153
StyD4I CCNGG 6 cut(s) 280, 333, 643, 1300, 1321, 1867
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 8 cut(s) 197, 406, 514, 981, 1220, 1682, 2419, 2631
TaiI ACGT 3 cut(s) 17, 105, 548
TaqI TCGA 4 cut(s) 468, 1423, 2382, 2845
TatI WGTACW 3 cut(s) 604, 1532, 2203
TauI GCSGC 3 cut(s) 54, 57, 1111
TfiI GAWTC 6 cut(s) 312, 500, 926, 1082, 1925, 2209
TscAI CASTG 8 cut(s) 511, 891, 964, 1126, 1225, 1681, 1687, 1993
TseFI GTSAC 8 cut(s) 106, 199, 451, 626, 931, 1723, 1759, 2413
Tsp45I GTSAC 8 cut(s) 106, 199, 451, 626, 931, 1723, 1759, 2413
TspGWI ACGGA 6 cut(s) 238, 250, 768, 1497, 2349, 2558
TspRI CASTG 8 cut(s) 511, 891, 964, 1126, 1225, 1681, 1687, 1993
Tth111I GACNNNGTC 1 cut(s) 197
VpaK11BI GGWCC 3 cut(s) 85, 446, 1715
VspI ATTAAT 1 cut(s) 2873
XagI CCTNNNNNAGG 1 cut(s) 1845
XapI RAATTY 3 cut(s) 290, 1805, 2122
XceI RCATGY 2 cut(s) 1354, 1700
XcmI CCANNNNNNNNNTGG 1 cut(s) 1557
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 4 cut(s) 1095, 1992, 2138, 2153
ZrmI AGTACT 1 cut(s) 1534
Zsp2I ATGCAT 1 cut(s) 2306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.