AT3G47180

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013443
Physical Location & Seq
Reverse (-)
17372731 .. 17373547
817 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G47180.1

Sequence Viewer

Length: 633 bp
ATGGATAACCAAGAAGAAGAGCCTAAGCAACCTCCAAACAAACTTCCCGATCTAGCCCTCTTTGAGCAAGCAAATTCTGAAGTTGCTCTTGCTGCCTCCCAAGCTAATTCCCATTTTGCCCATGCTATGCATGATTCGTCTCCCTCAATGATTTCAATGATAGAAAGTGACGAAGAATCTGAAGATGAAGAAGAAATCAATGAGAACTACTACGAGTATTTCGATAGCAATGGATTTGGAGTAGATGAAGACGAAATCAATGAGTTTCTTGAAGATCAAGAAAGCAATTCCAACCTTGAGGAGGAGGATGATTTCTTGGAGGAAGAAGATGAGATTGATCCCGACCAATTGTCTTACGAGGAACTGATTGCGCTTGGAGATTTCATCGGAGTCGAAAACCGTGGGCTAACTCCTATTGAAATCTCTACGTGTTTGAACGCATCTACATACGTATTTTCACATAACAAAAACGAGATTGATCGTTGTGTAGTTTGCCAAATGGAGTTTGAAGAAAGAGAATCTTTGGTCGTTCTCCGACCTTGTGACCATCCATACCACTCTGAGTGTATAACCAAATGGCTTGAGACAAAGAAGATTTGTCCCATTTGTTGTTCTGAACCATCTGTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

210

Amino Acids

24.02

Weight (kDa)

4.05

Isoelectric Point (pI)

71.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 160 - 203 3.2e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 161 - 203 1.1e-11 Ring finger domain
zf-C3HC4_2 PF13923 161 - 203 3.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 162 - 190 2.2e-06 RING-like zinc finger
zf-C3HC4 PF00097 162 - 203 7.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 1 cut(s) 73
AcuI CTGAAG 2 cut(s) 99, 201
AfiI CCNNNNNNNGG 1 cut(s) 301
AflIII ACRYGT 1 cut(s) 428
AgsI TTSAA 5 cut(s) 156, 272, 419, 436, 509
AhdI GACNNNNNGTC 1 cut(s) 349
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
Alw26I GTCTC 2 cut(s) 144, 578
AlwI GGATC 1 cut(s) 332
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 1 cut(s) 73
AspLEI GCGC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 79
BccI CCATC 2 cut(s) 555, 628
BcoDI GTCTC 2 cut(s) 144, 578
BfaI CTAG 1 cut(s) 53
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
BmeRI GACNNNNNGTC 1 cut(s) 349
BmsI GCATC 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 255
Bpu10I CCTNAGC 1 cut(s) 24
BpuEI CTTGAG 2 cut(s) 317, 602
BsaAI YACGTR 2 cut(s) 429, 451
BsaJI CCNNGG 1 cut(s) 400
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 2 cut(s) 313, 547
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMI GCAATG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 552
BseRI GAGGAG 2 cut(s) 314, 317
BseXI GCAGC 1 cut(s) 79
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 1 cut(s) 301
BsmAI GTCTC 2 cut(s) 144, 578
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 585
Bsp143I GATC 4 cut(s) 49, 274, 337, 478
BspCNI CTCAG 1 cut(s) 553
BspPI GGATC 1 cut(s) 332
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 235
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 4 cut(s) 49, 274, 337, 478
Bst4CI ACNGT 1 cut(s) 401
Bst6I CTCTTC 1 cut(s) 12
BstBAI YACGTR 2 cut(s) 429, 451
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 2 cut(s) 24, 561
BstDSI CCRYGG 1 cut(s) 400
BstENI CCTNNNNNAGG 1 cut(s) 299
BstF5I GGATG 2 cut(s) 313, 547
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 4 cut(s) 52, 277, 340, 481
BstMAI GTCTC 2 cut(s) 144, 578
BstMBI GATC 4 cut(s) 49, 274, 337, 478
BstMWI GCNNNNNNNGC 2 cut(s) 92, 101
BstSNI TACGTA 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 400
BtsCI GGATG 2 cut(s) 313, 547
Cac8I GCNNGC 1 cut(s) 69
CfoI GCGC 1 cut(s) 373
CviAII CATG 2 cut(s) 122, 131
CviJI RGCY 5 cut(s) 22, 56, 104, 406, 580
CviKI_1 RGCY 5 cut(s) 22, 56, 104, 406, 580
DdeI CTNAG 2 cut(s) 24, 561
DpnI GATC 4 cut(s) 51, 276, 339, 480
DpnII GATC 4 cut(s) 49, 274, 337, 478
DriI GACNNNNNGTC 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 12
Eam1105I GACNNNNNGTC 1 cut(s) 349
EarI CTCTTC 1 cut(s) 12
Eco105I TACGTA 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 99, 201
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoT22I ATGCAT 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 2 cut(s) 125, 134
FaiI YATR 7 cut(s) 123, 128, 132, 448, 462, 553, 569
FalI AAGNNNNNCTT 4 cut(s) 72, 104, 505, 537
FaqI GGGAC 1 cut(s) 585
FatI CATG 2 cut(s) 121, 130
Fnu4HI GCNGC 1 cut(s) 93
FokI GGATG 2 cut(s) 320, 534
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 372
GluI GCNGC 1 cut(s) 93
HhaI GCGC 1 cut(s) 373
Hin1II CATG 2 cut(s) 125, 134
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HinfI GANTC 4 cut(s) 134, 176, 390, 518
Hpy188I TCNGA 6 cut(s) 79, 181, 389, 536, 562, 616
Hpy188III TCNNGA 4 cut(s) 47, 269, 278, 341
HpyCH4III ACNGT 1 cut(s) 401
HpyCH4IV ACGT 2 cut(s) 428, 450
HpyCH4V TGCA 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 101
HpyF3I CTNAG 2 cut(s) 24, 561
HpySE526I ACGT 2 cut(s) 428, 450
Hsp92II CATG 2 cut(s) 125, 134
HspAI GCGC 1 cut(s) 371
Kzo9I GATC 4 cut(s) 49, 274, 337, 478
LguI GCTCTTC 1 cut(s) 12
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 1 cut(s) 449
MaeI CTAG 1 cut(s) 53
MaeII ACGT 2 cut(s) 428, 450
MaeIII GTNAC 2 cut(s) 167, 542
MalI GATC 4 cut(s) 51, 276, 339, 480
MboI GATC 4 cut(s) 49, 274, 337, 478
MfeI CAATTG 1 cut(s) 347
MluCI AATT 4 cut(s) 73, 106, 286, 347
MlyI GAGTC 1 cut(s) 399
MmeI TCCRAC 2 cut(s) 315, 559
MnlI CCTC 9 cut(s) 42, 68, 106, 154, 292, 295, 298, 313, 352
Mph1103I ATGCAT 1 cut(s) 132
MunI CAATTG 1 cut(s) 347
MwoI GCNNNNNNNGC 2 cut(s) 92, 101
NdeII GATC 4 cut(s) 49, 274, 337, 478
NlaIII CATG 2 cut(s) 125, 134
NmuCI GTSAC 2 cut(s) 167, 542
NsiI ATGCAT 1 cut(s) 132
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 3 cut(s) 134, 176, 518
PkrI GCNGC 1 cut(s) 94
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
Ppu21I YACGTR 2 cut(s) 429, 451
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 4 cut(s) 49, 274, 337, 478
SchI GAGTC 1 cut(s) 399
SetI ASST 6 cut(s) 34, 106, 297, 431, 453, 541
SfaNI GCATC 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 296, 581
SmoI CTYRAG 2 cut(s) 296, 581
SnaBI TACGTA 1 cut(s) 451
Sse9I AATT 4 cut(s) 73, 106, 286, 347
SspMI CTAG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 401
TaiI ACGT 2 cut(s) 431, 453
TaqI TCGA 2 cut(s) 222, 393
TasI AATT 4 cut(s) 73, 106, 286, 347
TfiI GAWTC 3 cut(s) 134, 176, 518
TseFI GTSAC 2 cut(s) 167, 542
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 2 cut(s) 167, 542
TspDTI ATGAA 3 cut(s) 201, 261, 373
XagI CCTNNNNNAGG 1 cut(s) 299
XapI RAATTY 1 cut(s) 73
XspI CTAG 1 cut(s) 53
Zsp2I ATGCAT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.