RchiOBHm_Chr6g0296291

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013443
Physical Location & Seq
Reverse (-)
58034442 .. 58035237
796 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26595

Sequence Viewer

Length: 660 bp
ATGGAGGGAGAAGAAGAGAGCAAGCAATCAGATCCGCGGAGCTTAATCCCTTTCTCCGAGCTCAACCAAGTCAGCTCGGATTTCATCCTAGCCATGACTATGCACGAACAAGAGCACGCATACAGGTTGCTCGAAACAATTGAAAGTGAAAGCGAAGAAGAAGATGAAGAAGAAGATGATGATGATCAAATTCATGATGTCGACGATGGTACTTCTTCCTCTGAGGAATTCTATCATCAAGATGATGCTGATTTCTTTGATCTTCTCAGTGACGATAATGGAAGCGACAGTGACCAAGAAATGGAGGAAGATGACTTTGGTGATCTGGACGTGGATGAACTAACTTATGAGGAATTGTTAGCACTTGGAGAGTTCATAGGGGAAGAGAAAAGAGGTCTGCCCAGCAATGAAATTTCTACATGTTTGCATCCCCACACATACAAATTATCTCCAATTTATCAGAGCAAGAATAGCATTATTGATAAGTGTGTTGTATGTCAGTATGAGTATGAAGATGGTGAGGCTTTGGCTGCATTGCCTTGTGAACACCATTATCATTTGGAGTGTATAAGCAACTGGCTTCAGATTAAAAAGTGTTGCCCTATTTGCAGCACAGAAGTTTCATCATCATCATCATCATCCAAAACCAAAATTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

219

Amino Acids

24.93

Weight (kDa)

4.05

Isoelectric Point (pI)

66.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 156 - 203 5.7e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 161 - 203 1.2e-12 Ring finger domain
zf-C3HC4_2 PF13923 162 - 203 3.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 163 - 190 1.2e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 301
AccI GTMKAC 1 cut(s) 201
AccII CGCG 1 cut(s) 37
AciI CCGC 2 cut(s) 35, 37
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 3 cut(s) 189, 227, 411
AcuI CTGAAG 1 cut(s) 566
AfaI GTAC 1 cut(s) 211
AfiI CCNNNNNNNGG 1 cut(s) 301
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 1 cut(s) 143
AjiI CACGTC 1 cut(s) 331
AjuI GAANNNNNNNTTGG 2 cut(s) 300, 332
AluBI AGCT 3 cut(s) 42, 61, 75
AluI AGCT 3 cut(s) 42, 61, 75
Alw21I GWGCWC 2 cut(s) 63, 117
AlwI GGATC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 530, 609
ApoI RAATTY 3 cut(s) 189, 227, 411
AsuHPI GGTGA 2 cut(s) 332, 530
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 63, 117
BbvI GCAGC 2 cut(s) 517, 621
BccI CCATC 2 cut(s) 200, 509
BclI TGATCA 1 cut(s) 184
BfaI CTAG 1 cut(s) 89
BisI GCNGC 2 cut(s) 531, 610
BlsI GCNGC 2 cut(s) 532, 611
BmgBI CACGTC 1 cut(s) 331
BmsI GCATC 2 cut(s) 235, 436
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 35
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 581
Bse3DI GCAATG 2 cut(s) 412, 533
Bse8I GATNNNNATC 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 35
BseGI GGATG 4 cut(s) 84, 340, 427, 638
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMI GCAATG 2 cut(s) 412, 533
BseMII CTCAG 2 cut(s) 213, 280
BseNI ACTGG 1 cut(s) 581
BseXI GCAGC 2 cut(s) 517, 621
BseYI CCCAGC 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 63, 117
BslI CCNNNNNNNGG 1 cut(s) 301
Bsp1286I GDGCHC 2 cut(s) 63, 117
Bsp143I GATC 4 cut(s) 31, 184, 259, 322
BspACI CCGC 2 cut(s) 35, 37
BspCNI CTCAG 2 cut(s) 214, 279
BspFNI CGCG 1 cut(s) 37
BspHI TCATGA 1 cut(s) 193
BspPI GGATC 1 cut(s) 26
BsrDI GCAATG 2 cut(s) 412, 533
BsrI ACTGG 1 cut(s) 581
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 4 cut(s) 31, 184, 259, 322
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 2 cut(s) 9, 378
BstC8I GCNNGC 2 cut(s) 23, 117
BstDEI CTNAG 2 cut(s) 222, 266
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 4 cut(s) 84, 340, 427, 638
BstFNI CGCG 1 cut(s) 37
BstKTI GATC 4 cut(s) 34, 187, 262, 325
BstMBI GATC 4 cut(s) 31, 184, 259, 322
BstMWI GCNNNNNNNGC 3 cut(s) 471, 530, 606
BstNSI RCATGY 1 cut(s) 423
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 517, 621
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BtgI CCRYGG 1 cut(s) 35
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 4 cut(s) 84, 340, 427, 638
BtsIMutI CAGTG 2 cut(s) 274, 295
Cac8I GCNNGC 2 cut(s) 23, 117
CciI TCATGA 1 cut(s) 193
Cfr42I CCGCGG 1 cut(s) 38
Csp6I GTAC 1 cut(s) 210
CviAII CATG 3 cut(s) 94, 194, 420
CviJI RGCY 7 cut(s) 42, 61, 75, 92, 524, 530, 580
CviKI_1 RGCY 7 cut(s) 42, 61, 75, 92, 524, 530, 580
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 2 cut(s) 222, 266
DpnI GATC 4 cut(s) 33, 186, 261, 324
DpnII GATC 4 cut(s) 31, 184, 259, 322
Eam1104I CTCTTC 2 cut(s) 9, 378
EarI CTCTTC 2 cut(s) 9, 378
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 63
FaeI CATG 3 cut(s) 97, 197, 423
FatI CATG 3 cut(s) 93, 193, 419
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 531, 610
FokI GGATG 4 cut(s) 71, 347, 414, 625
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 2 cut(s) 531, 610
FspBI CTAG 1 cut(s) 89
GluI GCNGC 2 cut(s) 531, 610
GsaI CCCAGC 1 cut(s) 405
Hin1II CATG 3 cut(s) 97, 197, 423
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HphI GGTGA 2 cut(s) 332, 530
Hpy166II GTNNAC 2 cut(s) 202, 545
Hpy188I TCNGA 6 cut(s) 31, 58, 79, 223, 462, 585
Hpy188III TCNNGA 3 cut(s) 194, 239, 326
Hpy8I GTNNAC 2 cut(s) 202, 545
Hpy99I CGWCG 1 cut(s) 206
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 4 cut(s) 103, 427, 533, 609
HpyF10VI GCNNNNNNNGC 3 cut(s) 471, 530, 606
HpyF3I CTNAG 2 cut(s) 222, 266
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 3 cut(s) 97, 197, 423
Ksp22I TGATCA 1 cut(s) 184
KspI CCGCGG 1 cut(s) 38
Kzo9I GATC 4 cut(s) 31, 184, 259, 322
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 4 cut(s) 109, 311, 415, 562
Lsp1109I GCAGC 2 cut(s) 517, 621
LweI GCATC 2 cut(s) 235, 436
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 2 cut(s) 269, 290
MalI GATC 4 cut(s) 33, 186, 261, 324
MboI GATC 4 cut(s) 31, 184, 259, 322
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 63, 117
MluCI AATT 8 cut(s) 138, 189, 227, 353, 411, 443, 453, 651
MnlI CCTC 6 cut(s) 217, 229, 298, 343, 386, 514
MseI TTAA 2 cut(s) 44, 588
MslI CAYNNNNRTG 2 cut(s) 98, 240
MspA1I CMGCKG 1 cut(s) 37
MunI CAATTG 1 cut(s) 138
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 3 cut(s) 471, 530, 606
NdeII GATC 4 cut(s) 31, 184, 259, 322
NlaIII CATG 3 cut(s) 97, 197, 423
NmuCI GTSAC 2 cut(s) 269, 290
NspI RCATGY 1 cut(s) 423
PagI TCATGA 1 cut(s) 193
PciI ACATGT 1 cut(s) 419
PflMI CCANNNNNTGG 1 cut(s) 301
PkrI GCNGC 2 cut(s) 532, 611
PscI ACATGT 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 401
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
RseI CAYNNNNRTG 2 cut(s) 98, 240
SacI GAGCTC 1 cut(s) 63
SacII CCGCGG 1 cut(s) 38
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 2 cut(s) 44, 588
SatI GCNGC 2 cut(s) 531, 610
Sau3AI GATC 4 cut(s) 31, 184, 259, 322
SduI GDGCHC 2 cut(s) 63, 117
SetI ASST 6 cut(s) 44, 63, 77, 128, 333, 397
SfaNI GCATC 2 cut(s) 235, 436
Sfr303I CCGCGG 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 38
SmiMI CAYNNNNRTG 2 cut(s) 98, 240
Sse9I AATT 8 cut(s) 138, 189, 227, 353, 411, 443, 453, 651
SsiI CCGC 2 cut(s) 35, 37
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 1 cut(s) 63
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 333
TaqI TCGA 2 cut(s) 132, 201
TasI AATT 8 cut(s) 138, 189, 227, 353, 411, 443, 453, 651
Tru1I TTAA 2 cut(s) 44, 588
Tru9I TTAA 2 cut(s) 44, 588
TscAI CASTG 2 cut(s) 274, 295
TseFI GTSAC 2 cut(s) 269, 290
TseI GCWGC 2 cut(s) 530, 609
Tsp45I GTSAC 2 cut(s) 269, 290
TspDTI ATGAA 8 cut(s) 73, 180, 182, 351, 364, 423, 525, 612
TspRI CASTG 2 cut(s) 274, 295
Van91I CCANNNNNTGG 1 cut(s) 301
XapI RAATTY 3 cut(s) 189, 227, 411
XceI RCATGY 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.