Rorug06G0260700

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013443
Physical Location & Seq
Forward (+)
40756091 .. 40758389
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0260700.1

Sequence Viewer

Length: 828 bp
ATGGGACACTTTTCAGCTACGGATGAGAATCTAGGATTCCAGTCTGGAGTTCCCATACCGGGACTGAATCCTCCATCAAATCAGTTTGGAAATGCATTTTGTGGAGCTGGGTCGGGACTCATTCGAGAGGGATTGGGTGCTTATGGGAAGAGAATTTGTGGATCAAGTTCACAGTATGTGCAAAGCAATATAATTAAGTGCTTCTCGGATCCTCAATACTACTTCCAAGTGAATGCGCACTATGTCAGGAACAAACTGAAGATAATTCTATTTCCATTTCTTCACAGGGGACACTGGACCAGGATAACAGAGCCAATTGGAGGCAGGCTTTCCTATAATCCTCCAATTAATGACATAAATGCTCCAGATTTATACATTCCATTCATGACATTTGCAACATACTTGGTTCTTGCTGGCATCTCTCTCGGCCTTTCTGGGAAATTTAGTCCAGAAGCCTTAAATTGGCAGTTTGTGAAAGGAATGGCAAGCTGGTTATTCCAAGCAATGTTGTTGAAGGCATCATTGACTTCGCTAGGCGGTGGAGAGGCACCATTGTTGGACATGATAGCATATGCTGGGTATACATTCACGGGGCTGTGTGTGGCTGTTGTAGGGAGAATTACATTAAGCTATGCGTACTACTTGATAGTCATATGGACATCCATGTGCTCAGGCATCTTCTTGGCTAAGACGATTAAGCTAACTTTGTATGCCGAGGCTAGGAGTTACGATGTCACCATCCATAACTATCTCTTGCTTGGTATTGCATTTTCTCCATTCCCATTCATCTTCTGCCTGAGCAACCCTACCGGGAATTGGATTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

275

Amino Acids

30.2

Weight (kDa)

8.83

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 47 - 244 1.5e-50 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 547
AccI GTMKAC 1 cut(s) 581
AciI CCGC 1 cut(s) 537
AclWI GGATC 3 cut(s) 169, 203, 216
AcsI RAATTY 2 cut(s) 153, 440
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 5 cut(s) 59, 320, 462, 720, 816
AgsI TTSAA 1 cut(s) 514
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 5 cut(s) 17, 107, 489, 630, 700
AluI AGCT 5 cut(s) 17, 107, 489, 630, 700
Alw21I GWGCWC 1 cut(s) 671
AlwI GGATC 3 cut(s) 169, 203, 216
AoxI GGCC 1 cut(s) 427
ApoI RAATTY 2 cut(s) 153, 440
AseI ATTAAT 1 cut(s) 348
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 1 cut(s) 297
AsuC2I CCSGG 2 cut(s) 60, 811
AsuHPI GGTGA 1 cut(s) 727
AvaII GGWCC 1 cut(s) 297
BamHI GGATCC 1 cut(s) 208
BanI GGYRCC 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 671
BccI CCATC 2 cut(s) 82, 746
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 1 cut(s) 301
BcnI CCSGG 2 cut(s) 60, 811
BfaI CTAG 3 cut(s) 32, 533, 720
Bme1390I CCNGG 3 cut(s) 60, 301, 811
Bme18I GGWCC 1 cut(s) 297
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 210, 549
BmrFI CCNGG 3 cut(s) 60, 301, 811
BmsI GCATC 3 cut(s) 426, 527, 684
BpmI CTGGAG 2 cut(s) 66, 348
Bpu10I CCTNAGC 2 cut(s) 670, 797
BpuMI CCSGG 2 cut(s) 60, 811
BsaBI GATNNNNATC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 714
Bsc4I CCNNNNNNNGG 5 cut(s) 59, 320, 462, 720, 816
Bse1I ACTGG 2 cut(s) 40, 299
Bse3DI GCAATG 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 714
BseGI GGATG 3 cut(s) 28, 659, 738
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 5 cut(s) 59, 320, 462, 720, 816
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 684, 788
BseNI ACTGG 2 cut(s) 40, 299
BseYI CCCAGC 2 cut(s) 107, 575
BshFI GGCC 1 cut(s) 429
BshNI GGYRCC 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 671
BsiSI CCGG 2 cut(s) 59, 810
BslFI GGGAC 4 cut(s) 18, 75, 129, 303
BslI CCNNNNNNNGG 5 cut(s) 59, 320, 462, 720, 816
BsmFI GGGAC 4 cut(s) 18, 75, 129, 303
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 671
Bsp143I GATC 2 cut(s) 161, 208
BspACI CCGC 1 cut(s) 537
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 2 cut(s) 683, 789
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 2 cut(s) 210, 549
BspPI GGATC 3 cut(s) 169, 203, 216
BspT107I GGYRCC 1 cut(s) 547
BsrDI GCAATG 1 cut(s) 510
BsrI ACTGG 2 cut(s) 40, 299
BssECI CCNNGG 1 cut(s) 714
BssMI GATC 2 cut(s) 161, 208
BssNAI GTATAC 1 cut(s) 582
Bst1107I GTATAC 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 143
BstC8I GCNNGC 3 cut(s) 326, 415, 487
BstDEI CTNAG 3 cut(s) 670, 687, 797
BstF5I GGATG 3 cut(s) 28, 659, 738
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 2 cut(s) 164, 211
BstMBI GATC 2 cut(s) 161, 208
BstNI CCWGG 1 cut(s) 301
BstSCI CCNGG 3 cut(s) 58, 299, 809
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BstZ17I GTATAC 1 cut(s) 582
BsuRI GGCC 1 cut(s) 429
BtsCI GGATG 3 cut(s) 28, 659, 738
BtsIMutI CAGTG 1 cut(s) 292
Cac8I GCNNGC 3 cut(s) 326, 415, 487
CciI TCATGA 1 cut(s) 384
CfoI GCGC 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 297
Csp6I GTAC 1 cut(s) 637
CviAII CATG 3 cut(s) 385, 562, 664
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 3 cut(s) 670, 687, 797
DpnI GATC 2 cut(s) 163, 210
DpnII GATC 2 cut(s) 161, 208
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 299
EcoT22I ATGCAT 1 cut(s) 97
FaeI CATG 3 cut(s) 388, 565, 667
FaqI GGGAC 4 cut(s) 18, 75, 129, 303
FatI CATG 3 cut(s) 384, 561, 663
FauNDI CATATG 2 cut(s) 571, 653
FblI GTMKAC 1 cut(s) 581
FokI GGATG 3 cut(s) 35, 646, 725
FspAI RTGCGCAY 1 cut(s) 237
FspBI CTAG 3 cut(s) 32, 533, 720
FspI TGCGCA 1 cut(s) 237
GlaI GCGC 1 cut(s) 237
GsaI CCCAGC 2 cut(s) 111, 579
GsuI CTGGAG 2 cut(s) 66, 348
HaeIII GGCC 1 cut(s) 429
HapII CCGG 2 cut(s) 59, 810
HhaI GCGC 1 cut(s) 238
Hin1II CATG 3 cut(s) 388, 565, 667
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HinfI GANTC 4 cut(s) 28, 36, 67, 117
HpaII CCGG 2 cut(s) 59, 810
HphI GGTGA 1 cut(s) 727
Hpy166II GTNNAC 2 cut(s) 170, 582
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 8 cut(s) 45, 114, 125, 247, 365, 385, 449, 825
Hpy8I GTNNAC 2 cut(s) 170, 582
HpyAV CCTTC 1 cut(s) 508
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 95, 181, 395, 767
HpyF3I CTNAG 3 cut(s) 670, 687, 797
Hsp92II CATG 3 cut(s) 388, 565, 667
HspAI GCGC 1 cut(s) 236
Kzo9I GATC 2 cut(s) 161, 208
LmnI GCTCC 2 cut(s) 104, 367
LweI GCATC 3 cut(s) 426, 527, 684
MaeI CTAG 3 cut(s) 32, 533, 720
MaeIII GTNAC 2 cut(s) 725, 733
MalI GATC 2 cut(s) 163, 210
MboI GATC 2 cut(s) 161, 208
MboII GAAGA 5 cut(s) 160, 271, 272, 670, 781
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 671
MluCI AATT 9 cut(s) 153, 192, 264, 315, 345, 440, 460, 618, 814
MlyI GAGTC 1 cut(s) 111
MmeI TCCRAC 1 cut(s) 537
MnlI CCTC 7 cut(s) 81, 121, 222, 314, 351, 538, 709
Mph1103I ATGCAT 1 cut(s) 97
MseI TTAA 5 cut(s) 195, 348, 458, 626, 696
MslI CAYNNNNRTG 1 cut(s) 664
MspI CCGG 2 cut(s) 59, 810
MspR9I CCNGG 3 cut(s) 60, 301, 811
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 301
NciI CCSGG 2 cut(s) 60, 811
NdeI CATATG 2 cut(s) 571, 653
NdeII GATC 2 cut(s) 161, 208
NlaIII CATG 3 cut(s) 388, 565, 667
NlaIV GGNNCC 2 cut(s) 210, 549
NmeAIII GCCGAG 2 cut(s) 405, 739
NmuCI GTSAC 1 cut(s) 733
NsbI TGCGCA 1 cut(s) 237
NsiI ATGCAT 1 cut(s) 97
PagI TCATGA 1 cut(s) 384
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 3 cut(s) 28, 36, 67
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PshBI ATTAAT 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 299
PspFI CCCAGC 2 cut(s) 107, 575
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 210, 549
PspPI GGNCC 1 cut(s) 297
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
RseI CAYNNNNRTG 1 cut(s) 664
SaqAI TTAA 5 cut(s) 195, 348, 458, 626, 696
Sau3AI GATC 2 cut(s) 161, 208
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 3 cut(s) 60, 301, 811
SduI GDGCHC 1 cut(s) 671
SetI ASST 5 cut(s) 19, 109, 491, 632, 702
SfaNI GCATC 3 cut(s) 426, 527, 684
SinI GGWCC 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 664
Sse9I AATT 9 cut(s) 153, 192, 264, 315, 345, 440, 460, 618, 814
SsiI CCGC 1 cut(s) 537
SspMI CTAG 3 cut(s) 32, 533, 720
StyD4I CCNGG 3 cut(s) 58, 299, 809
TaaI ACNGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 124
TasI AATT 9 cut(s) 153, 192, 264, 315, 345, 440, 460, 618, 814
TfiI GAWTC 3 cut(s) 28, 36, 67
Tru1I TTAA 5 cut(s) 195, 348, 458, 626, 696
Tru9I TTAA 5 cut(s) 195, 348, 458, 626, 696
TscAI CASTG 1 cut(s) 299
TseFI GTSAC 1 cut(s) 733
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 2 cut(s) 373, 775
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 1 cut(s) 299
VpaK11BI GGWCC 1 cut(s) 297
VspI ATTAAT 1 cut(s) 348
XapI RAATTY 2 cut(s) 153, 440
XmiI GTMKAC 1 cut(s) 581
XspI CTAG 3 cut(s) 32, 533, 720
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.