pycom08g13690

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013443
Physical Location & Seq
Forward (+)
12546940 .. 12547554
615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g13690.1

Sequence Viewer

Length: 615 bp
ATGAACGGAGAAGAAGATCAAGCCGAACAATCTTCCGGGAGGATCCCTTTCACCGAACTCATCCAAGTGAGCGCAGATTTCATTCTTGCCCTGGCTATGCAAGAGCAAGAGCAGGCCTACACCATGCTCGAAACCATAGAAAGCGATAGTGAAGAAGACGGTGCTGAAATTGATGATGCTTCTTCGTCCTCTAGTGAAAACTACGACCCAGACGTTGCTGCTTTCCTTGAAAGCCGAGAATTTGATGCTGATGATTTCAGGTCTCTTGAGGACGAAGAAATTGGCACAAGCAGTAGCAGCAGTGATCAAGAAACGGATGATTTGGACGTGGATGAACTAACTTATGAGGAATTGTTAGCACTTGGAGAATTCATAGGGGAAGAAAAAAGAGGACTTCCCAGCAGTGAGATTTCTGCATGTTTGCATCCCTACACGTGGGAATTAGCGGTCGGTCAGAGCAAGACTAGCATCGATCGATGCGTTGTTTGTCAGCTCGAGTATGACGACGGTGAATCACTAGCTGCATTGTCCTGTGAACACCCTTATCATTGGGAGTGTATAAGCCAGTGGCTTCAGATTAAAAAGAGTTGCCCTATTTGCAGCACACAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

205

Amino Acids

22.71

Weight (kDa)

4.05

Isoelectric Point (pI)

64.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 158 - 200 1.3e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 159 - 200 5.1e-13 Ring finger domain
zf-C3HC4_2 PF13923 159 - 200 8.9e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 446
AclWI GGATC 2 cut(s) 37, 50
AcsI RAATTY 2 cut(s) 239, 368
AcuI CTGAAG 1 cut(s) 557
AcvI CACGTG 1 cut(s) 435
AfiI CCNNNNNNNGG 1 cut(s) 435
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 1 cut(s) 230
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 2 cut(s) 493, 521
AluI AGCT 2 cut(s) 493, 521
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 2 cut(s) 37, 50
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 4 cut(s) 218, 297, 521, 600
ApoI RAATTY 2 cut(s) 239, 368
AspLEI GCGC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 2 cut(s) 43, 521
AvaI CYCGRG 1 cut(s) 494
BamHI GGATCC 1 cut(s) 42
BbrPI CACGTG 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 205, 309, 508
BciT130I CCWGG 1 cut(s) 92
BclI TGATCA 1 cut(s) 304
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 3 cut(s) 192, 465, 518
BisI GCNGC 4 cut(s) 219, 298, 522, 601
BlsI GCNGC 4 cut(s) 220, 299, 523, 602
Bme1390I CCNGG 2 cut(s) 37, 92
BmeT110I CYCGRG 1 cut(s) 494
BmgBI CACGTC 1 cut(s) 328
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 2 cut(s) 37, 92
BmsI GCATC 5 cut(s) 166, 235, 433, 467, 477
BpiI GAAGAC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 37
Bsa29I ATCGAT 2 cut(s) 471, 475
BsaAI YACGTR 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 435
Bse1I ACTGG 1 cut(s) 565
BseBI CCWGG 1 cut(s) 92
BseCI ATCGAT 2 cut(s) 471, 475
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 4 cut(s) 60, 322, 337, 424
BseLI CCNNNNNNNGG 1 cut(s) 435
BseNI ACTGG 1 cut(s) 565
BseXI GCAGC 3 cut(s) 205, 309, 508
BseYI CCCAGC 1 cut(s) 398
Bsh1285I CGRYCG 2 cut(s) 450, 475
BshFI GGCC 1 cut(s) 116
BshVI ATCGAT 2 cut(s) 471, 475
BsiEI CGRYCG 2 cut(s) 450, 475
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 435
BsmAI GTCTC 1 cut(s) 267
BsnI GGCC 1 cut(s) 116
Bso31I GGTCTC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 494
Bsp143I GATC 4 cut(s) 16, 42, 304, 472
BspACI CCGC 1 cut(s) 446
BspANI GGCC 1 cut(s) 116
BspDI ATCGAT 2 cut(s) 471, 475
BspLI GGNNCC 1 cut(s) 44
BspPI GGATC 2 cut(s) 37, 50
BspTNI GGTCTC 1 cut(s) 267
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 4 cut(s) 16, 42, 304, 472
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 2 cut(s) 161, 509
BstBAI YACGTR 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 114
BstF5I GGATG 4 cut(s) 60, 322, 337, 424
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 4 cut(s) 19, 45, 307, 475
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 4 cut(s) 16, 42, 304, 472
BstMCI CGRYCG 2 cut(s) 450, 475
BstMWI GCNNNNNNNGC 3 cut(s) 297, 465, 597
BstNI CCWGG 1 cut(s) 92
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 2 cut(s) 35, 90
BstV1I GCAGC 3 cut(s) 205, 309, 508
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
Bsu15I ATCGAT 2 cut(s) 471, 475
BsuRI GGCC 1 cut(s) 116
BsuTUI ATCGAT 2 cut(s) 471, 475
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 4 cut(s) 60, 322, 337, 424
BtsI GCAGTG 2 cut(s) 307, 409
BtsIMutI CAGTG 3 cut(s) 307, 409, 572
Cac8I GCNNGC 1 cut(s) 114
CfoI GCGC 1 cut(s) 74
ClaI ATCGAT 2 cut(s) 471, 475
CviAII CATG 2 cut(s) 124, 417
CviJI RGCY 8 cut(s) 23, 95, 116, 234, 493, 521, 564, 571
CviKI_1 RGCY 8 cut(s) 23, 95, 116, 234, 493, 521, 564, 571
DpnI GATC 4 cut(s) 18, 44, 306, 474
DpnII GATC 4 cut(s) 16, 42, 304, 472
Eco147I AGGCCT 1 cut(s) 116
Eco31I GGTCTC 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 557
Eco72I CACGTG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 127, 420
FaiI YATR 8 cut(s) 98, 125, 137, 345, 374, 418, 501, 560
FatI CATG 2 cut(s) 123, 416
FbaI TGATCA 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 219, 298, 522, 601
FokI GGATG 4 cut(s) 47, 329, 344, 411
Fsp4HI GCNGC 4 cut(s) 219, 298, 522, 601
FspBI CTAG 3 cut(s) 192, 465, 518
GlaI GCGC 1 cut(s) 73
GluI GCNGC 4 cut(s) 219, 298, 522, 601
GsaI CCCAGC 1 cut(s) 402
HaeIII GGCC 1 cut(s) 116
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 74
Hin1II CATG 2 cut(s) 127, 420
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 1 cut(s) 512
HpaII CCGG 1 cut(s) 36
HphI GGTGA 2 cut(s) 43, 521
Hpy166II GTNNAC 1 cut(s) 536
Hpy188I TCNGA 2 cut(s) 456, 576
Hpy188III TCNNGA 2 cut(s) 266, 308
Hpy8I GTNNAC 1 cut(s) 536
Hpy99I CGWCG 1 cut(s) 509
HpyCH4III ACNGT 2 cut(s) 161, 509
HpyCH4IV ACGT 3 cut(s) 213, 327, 434
HpyCH4V TGCA 5 cut(s) 100, 416, 424, 524, 600
HpyF10VI GCNNNNNNNGC 3 cut(s) 297, 465, 597
HpySE526I ACGT 3 cut(s) 213, 327, 434
Hsp92II CATG 2 cut(s) 127, 420
HspAI GCGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 4 cut(s) 16, 42, 304, 472
LpnPI CCDG 9 cut(s) 49, 77, 98, 104, 222, 244, 412, 544, 578
Lsp1109I GCAGC 3 cut(s) 205, 309, 508
LweI GCATC 5 cut(s) 166, 235, 433, 467, 477
MaeI CTAG 3 cut(s) 192, 465, 518
MaeII ACGT 3 cut(s) 213, 327, 434
MalI GATC 4 cut(s) 18, 44, 306, 474
MboI GATC 4 cut(s) 16, 42, 304, 472
MboII GAAGA 8 cut(s) 23, 24, 26, 164, 167, 174, 287, 392
MflI RGATCY 1 cut(s) 42
MluCI AATT 6 cut(s) 168, 239, 279, 350, 368, 440
MnlI CCTC 5 cut(s) 33, 199, 262, 340, 383
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 1 cut(s) 65
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 37, 92
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 297, 465, 597
NciI CCSGG 1 cut(s) 37
NdeII GATC 4 cut(s) 16, 42, 304, 472
NlaIII CATG 2 cut(s) 127, 420
NlaIV GGNNCC 1 cut(s) 44
NmeAIII GCCGAG 1 cut(s) 260
NspI RCATGY 1 cut(s) 420
PaeR7I CTCGAG 1 cut(s) 494
PceI AGGCCT 1 cut(s) 116
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 477, 501
PfeI GAWTC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 35
PkrI GCNGC 4 cut(s) 220, 299, 523, 602
Ple19I CGATCG 1 cut(s) 475
PmaCI CACGTG 1 cut(s) 435
PmlI CACGTG 1 cut(s) 435
Ppu21I YACGTR 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 90
PspCI CACGTG 1 cut(s) 435
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 44
PspXI VCTCGAGB 1 cut(s) 494
PsuI RGATCY 1 cut(s) 42
PvuI CGATCG 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 4 cut(s) 219, 298, 522, 601
Sau3AI GATC 4 cut(s) 16, 42, 304, 472
ScrFI CCNGG 2 cut(s) 37, 92
SetI ASST 6 cut(s) 216, 263, 330, 437, 495, 523
SfaNI GCATC 5 cut(s) 166, 235, 433, 467, 477
Sfr274I CTCGAG 1 cut(s) 494
SlaI CTCGAG 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 2 cut(s) 266, 494
SmoI CTYRAG 2 cut(s) 266, 494
Sse9I AATT 6 cut(s) 168, 239, 279, 350, 368, 440
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 1 cut(s) 446
SspMI CTAG 3 cut(s) 192, 465, 518
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 2 cut(s) 35, 90
TaaI ACNGT 2 cut(s) 161, 509
TaiI ACGT 3 cut(s) 216, 330, 437
TaqI TCGA 4 cut(s) 129, 471, 475, 495
TaqII GACCGA 1 cut(s) 440
TasI AATT 6 cut(s) 168, 239, 279, 350, 368, 440
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 3 cut(s) 307, 409, 572
TseI GCWGC 4 cut(s) 218, 297, 521, 600
TspDTI ATGAA 4 cut(s) 17, 70, 348, 361
TspGWI ACGGA 2 cut(s) 21, 329
TspRI CASTG 3 cut(s) 307, 409, 572
XapI RAATTY 2 cut(s) 239, 368
XceI RCATGY 1 cut(s) 420
XhoI CTCGAG 1 cut(s) 494
XspI CTAG 3 cut(s) 192, 465, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.