Prupe.1G493000_v2.0.a1

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013443
Physical Location & Seq
Forward (+)
40974389 .. 40975661
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G493000.1

Sequence Viewer

Length: 690 bp
ATGAATTGGGTTTTTGGCACTGTGCTCCTGGAAGAGATTGATCGTAGTTTTTCGATGGATGGCGGAGAAGATCAAAGCAAGCAATCTACTAGGAGAACCCCTTTCACTGAACTCAACCAAGTCAGCTCGGATTTCATTCTAGCCATGGCAATGCAAGAACAAGAGCAGGCGTACACAATGCTGGAAACCATCGAAAGCGATAGCAGCGACGAAGAAGAAGATGATGCTTCCTCCCCTAATGAAAACTACGATCAAGACGATACTGCTTTCTTTGAAAGCAGAGAATTCGAAACAGAGTTCGGGTTTCTTGCGAACGAAGACAGTGGCAGCAGCAGTGACCAAGAAATGGATGAAGATGATTTTGATGTGGATGAGCTAACTTATGAGGAGCTGATTGCACTTGGAGAGTTCATAGGGGAAGAGAAAAGAGGCCTGCCCAGCAATGAAATTTCCACATGTTTGCAGCCCTACACAAGCAAATCACCATCATCTATTCAAAGCAAGACCAGCATTGACAGGTGCGTGATTTGCCAGATTGAGTATGAAGATGGAGAAGCCCTAGTTGCATTGTCTTGCCAACACCCTTATCATTGGGATTGTATAAGCAAGTGGCTTGAGATTAAAAAGAGCTGCCCAATTTGCAGCACAGAAGTCTCATCAACATCATCATCCAAAACCAAGAACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

230

Amino Acids

25.89

Weight (kDa)

4.05

Isoelectric Point (pI)

70.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 346
AciI CCGC 1 cut(s) 63
AcsI RAATTY 2 cut(s) 284, 447
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 1 cut(s) 346
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 2 cut(s) 275, 497
AjnI CCWGG 1 cut(s) 27
AluBI AGCT 4 cut(s) 126, 376, 391, 630
AluI AGCT 4 cut(s) 126, 376, 391, 630
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 658
AoxI GGCC 1 cut(s) 430
ApeKI GCWGC 6 cut(s) 204, 327, 330, 463, 630, 642
ApoI RAATTY 2 cut(s) 284, 447
AsuHPI GGTGA 1 cut(s) 474
AsuII TTCGAA 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 6 cut(s) 216, 339, 342, 475, 617, 654
BccI CCATC 5 cut(s) 49, 53, 197, 493, 542
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 658
BfaI CTAG 3 cut(s) 90, 140, 560
BisI GCNGC 6 cut(s) 205, 328, 331, 464, 631, 643
BlsI GCNGC 6 cut(s) 206, 329, 332, 465, 632, 644
Bme1390I CCNGG 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 1 cut(s) 214
BpiI GAAGAC 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 635
BsaJI CCNNGG 1 cut(s) 144
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse3DI GCAATG 2 cut(s) 156, 448
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 4 cut(s) 64, 355, 376, 668
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 2 cut(s) 156, 448
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 6 cut(s) 216, 339, 342, 475, 617, 654
BseYI CCCAGC 1 cut(s) 437
BshFI GGCC 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 658
BsnI GGCC 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 3 cut(s) 40, 70, 250
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 1 cut(s) 63
BspANI GGCC 1 cut(s) 432
BspT104I TTCGAA 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 156, 448
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 3 cut(s) 40, 70, 250
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 2 cut(s) 22, 323
Bst6I CTCTTC 2 cut(s) 27, 414
BstBI TTCGAA 1 cut(s) 288
BstC8I GCNNGC 3 cut(s) 80, 168, 434
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 4 cut(s) 64, 355, 376, 668
BstKTI GATC 3 cut(s) 43, 73, 253
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 3 cut(s) 40, 70, 250
BstMWI GCNNNNNNNGC 6 cut(s) 204, 438, 507, 528, 563, 639
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 27
BstV1I GCAGC 6 cut(s) 216, 339, 342, 475, 617, 654
BstV2I GAAGAC 1 cut(s) 324
BsuRI GGCC 1 cut(s) 432
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 4 cut(s) 64, 355, 376, 668
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 4 cut(s) 18, 105, 328, 340
Cac8I GCNNGC 3 cut(s) 80, 168, 434
Csp6I GTAC 1 cut(s) 172
CviAII CATG 2 cut(s) 145, 456
CviJI RGCY 9 cut(s) 126, 143, 376, 391, 432, 466, 557, 613, 630
CviKI_1 RGCY 9 cut(s) 126, 143, 376, 391, 432, 466, 557, 613, 630
CviQI GTAC 1 cut(s) 172
DpnI GATC 3 cut(s) 42, 72, 252
DpnII GATC 3 cut(s) 40, 70, 250
Eam1104I CTCTTC 2 cut(s) 27, 414
EarI CTCTTC 2 cut(s) 27, 414
EciI GGCGGA 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 144
Eco147I AGGCCT 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 2 cut(s) 148, 459
FaiI YATR 8 cut(s) 146, 384, 413, 457, 543, 602, 686, 688
FatI CATG 2 cut(s) 144, 455
Fnu4HI GCNGC 6 cut(s) 205, 328, 331, 464, 631, 643
FokI GGATG 4 cut(s) 71, 362, 383, 655
Fsp4HI GCNGC 6 cut(s) 205, 328, 331, 464, 631, 643
FspBI CTAG 3 cut(s) 90, 140, 560
GluI GCNGC 6 cut(s) 205, 328, 331, 464, 631, 643
GsaI CCCAGC 1 cut(s) 441
HaeIII GGCC 1 cut(s) 432
Hin1II CATG 2 cut(s) 148, 459
HphI GGTGA 1 cut(s) 474
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 1 cut(s) 174
Hpy99I CGWCG 1 cut(s) 212
HpyCH4III ACNGT 2 cut(s) 22, 323
HpyCH4V TGCA 5 cut(s) 154, 398, 463, 566, 642
HpyF10VI GCNNNNNNNGC 6 cut(s) 204, 438, 507, 528, 563, 639
Hsp92II CATG 2 cut(s) 148, 459
Kzo9I GATC 3 cut(s) 40, 70, 250
LmnI GCTCC 2 cut(s) 30, 388
LpnPI CCDG 9 cut(s) 14, 41, 152, 167, 446, 451, 502, 520, 545
Lsp1109I GCAGC 6 cut(s) 216, 339, 342, 475, 617, 654
LweI GCATC 1 cut(s) 214
MaeI CTAG 3 cut(s) 90, 140, 560
MaeIII GTNAC 1 cut(s) 335
MalI GATC 3 cut(s) 42, 72, 252
MboI GATC 3 cut(s) 40, 70, 250
MboII GAAGA 9 cut(s) 44, 80, 224, 227, 230, 329, 365, 431, 557
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 4 cut(s) 4, 284, 447, 636
MnlI CCTC 3 cut(s) 241, 379, 422
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MwoI GCNNNNNNNGC 6 cut(s) 204, 438, 507, 528, 563, 639
NcoI CCATGG 1 cut(s) 144
NdeII GATC 3 cut(s) 40, 70, 250
NlaIII CATG 2 cut(s) 148, 459
NmuCI GTSAC 1 cut(s) 335
NspI RCATGY 1 cut(s) 459
NspV TTCGAA 1 cut(s) 288
PceI AGGCCT 1 cut(s) 432
PciI ACATGT 1 cut(s) 455
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PflMI CCANNNNNTGG 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 6 cut(s) 206, 329, 332, 465, 632, 644
PscI ACATGT 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 437
PspGI CCWGG 1 cut(s) 27
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 6 cut(s) 205, 328, 331, 464, 631, 643
Sau3AI GATC 3 cut(s) 40, 70, 250
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 1 cut(s) 27
SetI ASST 5 cut(s) 128, 378, 393, 521, 632
SfaNI GCATC 1 cut(s) 214
SfuI TTCGAA 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
Sse9I AATT 4 cut(s) 4, 284, 447, 636
SseBI AGGCCT 1 cut(s) 432
SsiI CCGC 1 cut(s) 63
SspMI CTAG 3 cut(s) 90, 140, 560
StuI AGGCCT 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 22, 323
TaqI TCGA 3 cut(s) 53, 192, 288
TasI AATT 4 cut(s) 4, 284, 447, 636
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 4 cut(s) 25, 112, 328, 340
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 6 cut(s) 204, 327, 330, 463, 630, 642
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 7 cut(s) 17, 124, 255, 366, 400, 459, 558
TspRI CASTG 4 cut(s) 25, 112, 328, 340
Van91I CCANNNNNTGG 1 cut(s) 346
XapI RAATTY 2 cut(s) 284, 447
XceI RCATGY 1 cut(s) 459
XspI CTAG 3 cut(s) 90, 140, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.