Rmu_sc0004708.1_g000007

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004708.1
OG0013443
Physical Location & Seq
Forward (+)
19102 .. 19761
660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004708.1_g000007.1.cds

Sequence Viewer

Length: 660 bp
atggagggagaagaagagagcaagcaatcagatccgcggagcttaatccctttctccgagctcaaccaagtcagctcggatttcatcctagccatgactatgcacgaacaagagcacgcatacaggatgctcgaaacaattgaaagtgaaagcgaagaagaagatgaagaagatgatgatgatcaaattcatgatgtcgacgatggtacttcttcctctgaggaattctatcatcaagatgatgctgatttccttgatcttctcagtgacgataatggaagcgacagtgaccaagaaatggaggaagatgactttggtgatctggacgtggatgaactaacttatgaggaattgttagcacttggagagttcataggggaagagaaaagaggtctgcccagcaatgaaatttctacatgtttgcatccccacacatacaaattatctccaatttatcagagcaagaatagcattattgataagtgtgttgtatgtcagtatgagtatgaagatggtgaggctttggctgcattgccttgtgaacaccattatcatttggagtgtataagcaactggcttcagattaaaaagtgttgccctatttgcagcacagaagtttcatcatcatcgtcatcatcatccaaaaccaaaattgtatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

219

Amino Acids

24.87

Weight (kDa)

4.05

Isoelectric Point (pI)

64.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 298
AccI GTMKAC 1 cut(s) 198
AccII CGCG 1 cut(s) 37
AciI CCGC 2 cut(s) 35, 37
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 3 cut(s) 186, 224, 408
AcuI CTGAAG 1 cut(s) 563
AfaI GTAC 1 cut(s) 208
AfiI CCNNNNNNNGG 1 cut(s) 298
AflIII ACRYGT 1 cut(s) 416
AgsI TTSAA 1 cut(s) 143
AjiI CACGTC 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 3 cut(s) 42, 61, 75
AluI AGCT 3 cut(s) 42, 61, 75
Alw21I GWGCWC 2 cut(s) 63, 117
AlwI GGATC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 527, 606
ApoI RAATTY 3 cut(s) 186, 224, 408
AsuHPI GGTGA 2 cut(s) 329, 527
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 63, 117
BbvI GCAGC 2 cut(s) 514, 618
BccI CCATC 2 cut(s) 197, 506
BclI TGATCA 1 cut(s) 181
BfaI CTAG 1 cut(s) 89
BisI GCNGC 2 cut(s) 528, 607
BlsI GCNGC 2 cut(s) 529, 608
BmgBI CACGTC 1 cut(s) 328
BmsI GCATC 3 cut(s) 117, 232, 433
BsaBI GATNNNNATC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 35
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 578
Bse3DI GCAATG 2 cut(s) 409, 530
Bse8I GATNNNNATC 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 35
BseGI GGATG 5 cut(s) 84, 132, 337, 424, 638
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 2 cut(s) 409, 530
BseMII CTCAG 2 cut(s) 210, 277
BseNI ACTGG 1 cut(s) 578
BseXI GCAGC 2 cut(s) 514, 618
BseYI CCCAGC 1 cut(s) 398
Bsh1236I CGCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 63, 117
BslI CCNNNNNNNGG 1 cut(s) 298
Bsp1286I GDGCHC 2 cut(s) 63, 117
Bsp143I GATC 4 cut(s) 31, 181, 256, 319
BspACI CCGC 2 cut(s) 35, 37
BspCNI CTCAG 2 cut(s) 211, 276
BspFNI CGCG 1 cut(s) 37
BspHI TCATGA 1 cut(s) 190
BspPI GGATC 1 cut(s) 26
BsrDI GCAATG 2 cut(s) 409, 530
BsrI ACTGG 1 cut(s) 578
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 4 cut(s) 31, 181, 256, 319
Bst4CI ACNGT 1 cut(s) 287
Bst6I CTCTTC 2 cut(s) 9, 375
BstC8I GCNNGC 2 cut(s) 23, 117
BstDEI CTNAG 2 cut(s) 219, 263
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 5 cut(s) 84, 132, 337, 424, 638
BstFNI CGCG 1 cut(s) 37
BstKTI GATC 4 cut(s) 34, 184, 259, 322
BstMBI GATC 4 cut(s) 31, 181, 256, 319
BstMWI GCNNNNNNNGC 3 cut(s) 468, 527, 603
BstNSI RCATGY 1 cut(s) 420
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 514, 618
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BtgI CCRYGG 1 cut(s) 35
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 5 cut(s) 84, 132, 337, 424, 638
BtsIMutI CAGTG 2 cut(s) 271, 292
Cac8I GCNNGC 2 cut(s) 23, 117
CciI TCATGA 1 cut(s) 190
Cfr42I CCGCGG 1 cut(s) 38
Csp6I GTAC 1 cut(s) 207
CviAII CATG 3 cut(s) 94, 191, 417
CviJI RGCY 7 cut(s) 42, 61, 75, 92, 521, 527, 577
CviKI_1 RGCY 7 cut(s) 42, 61, 75, 92, 521, 527, 577
CviQI GTAC 1 cut(s) 207
DdeI CTNAG 2 cut(s) 219, 263
DpnI GATC 4 cut(s) 33, 183, 258, 321
DpnII GATC 4 cut(s) 31, 181, 256, 319
Eam1104I CTCTTC 2 cut(s) 9, 375
EarI CTCTTC 2 cut(s) 9, 375
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 224
EcoT38I GRGCYC 1 cut(s) 63
FaeI CATG 3 cut(s) 97, 194, 420
FatI CATG 3 cut(s) 93, 190, 416
FbaI TGATCA 1 cut(s) 181
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 2 cut(s) 528, 607
FokI GGATG 5 cut(s) 71, 139, 344, 411, 625
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 2 cut(s) 528, 607
FspBI CTAG 1 cut(s) 89
GluI GCNGC 2 cut(s) 528, 607
GsaI CCCAGC 1 cut(s) 402
Hin1II CATG 3 cut(s) 97, 194, 420
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HphI GGTGA 2 cut(s) 329, 527
Hpy166II GTNNAC 2 cut(s) 199, 542
Hpy188I TCNGA 6 cut(s) 31, 58, 79, 220, 459, 582
Hpy188III TCNNGA 3 cut(s) 191, 236, 323
Hpy8I GTNNAC 2 cut(s) 199, 542
Hpy99I CGWCG 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 4 cut(s) 103, 424, 530, 606
HpyF10VI GCNNNNNNNGC 3 cut(s) 468, 527, 603
HpyF3I CTNAG 2 cut(s) 219, 263
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 3 cut(s) 97, 194, 420
Ksp22I TGATCA 1 cut(s) 181
KspI CCGCGG 1 cut(s) 38
Kzo9I GATC 4 cut(s) 31, 181, 256, 319
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 4 cut(s) 109, 308, 412, 559
Lsp1109I GCAGC 2 cut(s) 514, 618
LweI GCATC 3 cut(s) 117, 232, 433
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 266, 287
MalI GATC 4 cut(s) 33, 183, 258, 321
MboI GATC 4 cut(s) 31, 181, 256, 319
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 63, 117
MluCI AATT 8 cut(s) 138, 186, 224, 350, 408, 440, 450, 651
MnlI CCTC 6 cut(s) 214, 226, 295, 340, 383, 511
MseI TTAA 2 cut(s) 44, 585
MslI CAYNNNNRTG 2 cut(s) 98, 237
MspA1I CMGCKG 1 cut(s) 37
MunI CAATTG 1 cut(s) 138
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 3 cut(s) 468, 527, 603
NdeII GATC 4 cut(s) 31, 181, 256, 319
NlaIII CATG 3 cut(s) 97, 194, 420
NmuCI GTSAC 2 cut(s) 266, 287
NspI RCATGY 1 cut(s) 420
PagI TCATGA 1 cut(s) 190
PciI ACATGT 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 298
PkrI GCNGC 2 cut(s) 529, 608
PscI ACATGT 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 398
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 208
RsaNI GTAC 1 cut(s) 207
RseI CAYNNNNRTG 2 cut(s) 98, 237
SacI GAGCTC 1 cut(s) 63
SacII CCGCGG 1 cut(s) 38
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 44, 585
SatI GCNGC 2 cut(s) 528, 607
Sau3AI GATC 4 cut(s) 31, 181, 256, 319
SduI GDGCHC 2 cut(s) 63, 117
SetI ASST 5 cut(s) 44, 63, 77, 330, 394
SfaNI GCATC 3 cut(s) 117, 232, 433
Sfr303I CCGCGG 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 38
SmiMI CAYNNNNRTG 2 cut(s) 98, 237
Sse9I AATT 8 cut(s) 138, 186, 224, 350, 408, 440, 450, 651
SsiI CCGC 2 cut(s) 35, 37
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 1 cut(s) 63
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 1 cut(s) 330
TaqI TCGA 2 cut(s) 132, 198
TasI AATT 8 cut(s) 138, 186, 224, 350, 408, 440, 450, 651
Tru1I TTAA 2 cut(s) 44, 585
Tru9I TTAA 2 cut(s) 44, 585
TscAI CASTG 2 cut(s) 271, 292
TseFI GTSAC 2 cut(s) 266, 287
TseI GCWGC 2 cut(s) 527, 606
Tsp45I GTSAC 2 cut(s) 266, 287
TspDTI ATGAA 8 cut(s) 73, 179, 180, 348, 361, 420, 522, 609
TspRI CASTG 2 cut(s) 271, 292
Van91I CCANNNNNTGG 1 cut(s) 298
XapI RAATTY 3 cut(s) 186, 224, 408
XceI RCATGY 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.