FvH4_2g27450

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013443
Physical Location & Seq
Reverse (-)
21793431 .. 21794081
651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27450.t1

Sequence Viewer

Length: 651 bp
ATGGAGGGAGAAGAAGAGACCAAGCAATCAGATCCCCGGAGCTTAATCCCTATCTCCGAGCTCAACCAAGTCAGCTCGGATTTCATCCTAGCCATGACTGTGCACGAACAAGAGCAGGCGTACAGGATGCTTGAAACAATTGAAAGCGAAAGCGAAGAAGAAGATGATGATGATCAAATTCATGATGTCGATGATGATACTTCTTCCTCTGATGAATTCTATCGTCAAGATGATGCTGATTTCTTTGAGCTTCTCGAGGACGAAAATGGAAGCGGCAGTGACCAAGAAATGGAGGAAGATGATTTTGGTGATCTAGATGTGGATGAACTGACTTACGAGGAATTGTTAGCACTTGGAGAGTTCATAGGGGTAGAGAAAAGAGGTCTGCCCAGCAATGAAATTTCTACATGTTTGCATCCCCATACATACAGACTATCTCCAGTTCATCAGAGCAAGAATAGCGTTATTGATAAGTGTGTGGTATGTCAATACGAGTATGAAGATGGGGAAGCTTTGGCTGCATTGCCTTGTGAACACTCTTATCATTTGGAGTGTATCAGCAACTGGCTTCAGATCAAAAAATGTTGCCCGATTTGCAGCACAGAAGTTTCATCGTCGTCGTCGTCATCAGCCAAAACCAAAATTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

217

Amino Acids

24.44

Weight (kDa)

4.05

Isoelectric Point (pI)

54.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 152 - 199 7.6e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 157 - 199 3.1e-12 Ring finger domain
zf-C3HC4_2 PF13923 158 - 199 5.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 159 - 186 5.6e-08 RING-like zinc finger
zf-C3HC4 PF00097 159 - 199 9.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 1 cut(s) 273
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 4 cut(s) 177, 215, 399, 642
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 1 cut(s) 407
AgsI TTSAA 2 cut(s) 134, 143
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AluBI AGCT 5 cut(s) 42, 61, 75, 250, 512
AluI AGCT 5 cut(s) 42, 61, 75, 250, 512
Alw21I GWGCWC 2 cut(s) 63, 105
Alw26I GTCTC 1 cut(s) 11
Alw44I GTGCAC 1 cut(s) 101
AlwI GGATC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 564
Ama87I CYCGRG 1 cut(s) 254
ApaLI GTGCAC 1 cut(s) 101
ApeKI GCWGC 2 cut(s) 518, 597
ApoI RAATTY 4 cut(s) 177, 215, 399, 642
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 320
AvaI CYCGRG 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 63, 105
BbvI GCAGC 2 cut(s) 505, 609
BccI CCATC 1 cut(s) 497
BclI TGATCA 1 cut(s) 172
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 2 cut(s) 89, 314
BfmI CTRYAG 1 cut(s) 647
BisI GCNGC 3 cut(s) 274, 519, 598
BlsI GCNGC 3 cut(s) 275, 520, 599
Bme1390I CCNGG 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 37
BmsI GCATC 3 cut(s) 117, 223, 424
BpmI CTGGAG 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 37
BsaBI GATNNNNATC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 35
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse1I ACTGG 2 cut(s) 440, 569
Bse3DI GCAATG 2 cut(s) 400, 521
Bse8I GATNNNNATC 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 35
BseGI GGATG 4 cut(s) 84, 132, 328, 415
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 2 cut(s) 400, 521
BseNI ACTGG 2 cut(s) 440, 569
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 2 cut(s) 505, 609
BseYI CCCAGC 1 cut(s) 389
BsiHKAI GWGCWC 2 cut(s) 63, 105
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 1 cut(s) 11
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 63, 105
Bsp143I GATC 4 cut(s) 31, 172, 310, 573
BspACI CCGC 1 cut(s) 273
BspHI TCATGA 1 cut(s) 181
BspPI GGATC 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 400, 521
BsrI ACTGG 2 cut(s) 440, 569
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 4 cut(s) 31, 172, 310, 573
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 117
BstF5I GGATG 4 cut(s) 84, 132, 328, 415
BstKTI GATC 4 cut(s) 34, 175, 313, 576
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 4 cut(s) 31, 172, 310, 573
BstMWI GCNNNNNNNGC 3 cut(s) 459, 518, 594
BstNSI RCATGY 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 35
BstSFI CTRYAG 1 cut(s) 647
BstSLI GKGCMC 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 505, 609
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BtsCI GGATG 4 cut(s) 84, 132, 328, 415
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 283
Cac8I GCNNGC 1 cut(s) 117
CaiI CAGNNNCTG 1 cut(s) 564
CciI TCATGA 1 cut(s) 181
Csp6I GTAC 1 cut(s) 121
CviAII CATG 3 cut(s) 94, 182, 408
CviJI RGCY 9 cut(s) 42, 61, 75, 92, 250, 512, 518, 568, 632
CviKI_1 RGCY 9 cut(s) 42, 61, 75, 92, 250, 512, 518, 568, 632
CviQI GTAC 1 cut(s) 121
DpnI GATC 4 cut(s) 33, 174, 312, 575
DpnII GATC 4 cut(s) 31, 172, 310, 573
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 11
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 215
EcoT38I GRGCYC 1 cut(s) 63
FaeI CATG 3 cut(s) 97, 185, 411
FaiI YATR 9 cut(s) 95, 183, 365, 409, 423, 427, 484, 498, 649
FatI CATG 3 cut(s) 93, 181, 407
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 3 cut(s) 274, 519, 598
FokI GGATG 4 cut(s) 71, 139, 335, 402
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 3 cut(s) 274, 519, 598
FspBI CTAG 2 cut(s) 89, 314
GluI GCNGC 3 cut(s) 274, 519, 598
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 423
HapII CCGG 1 cut(s) 37
Hin1II CATG 3 cut(s) 97, 185, 411
HindIII AAGCTT 1 cut(s) 510
HpaII CCGG 1 cut(s) 37
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 2 cut(s) 103, 533
Hpy188I TCNGA 6 cut(s) 31, 58, 79, 211, 450, 573
Hpy188III TCNNGA 4 cut(s) 182, 227, 254, 314
Hpy8I GTNNAC 2 cut(s) 103, 533
Hpy99I CGWCG 3 cut(s) 619, 622, 625
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4V TGCA 4 cut(s) 103, 415, 521, 597
HpyF10VI GCNNNNNNNGC 3 cut(s) 459, 518, 594
Hsp92II CATG 3 cut(s) 97, 185, 411
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 4 cut(s) 31, 172, 310, 573
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 6 cut(s) 50, 101, 109, 403, 453, 550
Lsp1109I GCAGC 2 cut(s) 505, 609
LweI GCATC 3 cut(s) 117, 223, 424
MaeI CTAG 2 cut(s) 89, 314
MaeIII GTNAC 1 cut(s) 278
MalI GATC 4 cut(s) 33, 174, 312, 575
MboI GATC 4 cut(s) 31, 172, 310, 573
MboII GAAGA 8 cut(s) 23, 26, 167, 170, 173, 195, 308, 512
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 63, 105
MluCI AATT 6 cut(s) 138, 177, 215, 341, 399, 642
MnlI CCTC 5 cut(s) 217, 250, 286, 331, 374
MseI TTAA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 37
MunI CAATTG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 459, 518, 594
NciI CCSGG 1 cut(s) 37
NdeII GATC 4 cut(s) 31, 172, 310, 573
NlaIII CATG 3 cut(s) 97, 185, 411
NmuCI GTSAC 1 cut(s) 278
NspI RCATGY 1 cut(s) 411
PaeR7I CTCGAG 1 cut(s) 254
PagI TCATGA 1 cut(s) 181
PciI ACATGT 1 cut(s) 407
PcsI WCGNNNNNNNCGW 1 cut(s) 620
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 3 cut(s) 275, 520, 599
PscI ACATGT 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 564
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 1 cut(s) 98
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 3 cut(s) 274, 519, 598
Sau3AI GATC 4 cut(s) 31, 172, 310, 573
ScrFI CCNGG 1 cut(s) 37
SduI GDGCHC 2 cut(s) 63, 105
SetI ASST 6 cut(s) 44, 63, 77, 252, 385, 514
SfaNI GCATC 3 cut(s) 117, 223, 424
SfcI CTRYAG 1 cut(s) 647
Sfr274I CTCGAG 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 6 cut(s) 138, 177, 215, 341, 399, 642
SsiI CCGC 1 cut(s) 273
SspMI CTAG 2 cut(s) 89, 314
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 189, 255
TasI AATT 6 cut(s) 138, 177, 215, 341, 399, 642
TauI GCSGC 1 cut(s) 276
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 2 cut(s) 518, 597
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 9 cut(s) 73, 170, 228, 339, 352, 411, 434, 513, 600
TspRI CASTG 1 cut(s) 283
Van91I CCANNNNNTGG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 101
XapI RAATTY 4 cut(s) 177, 215, 399, 642
XbaI TCTAGA 1 cut(s) 313
XceI RCATGY 1 cut(s) 411
XhoI CTCGAG 1 cut(s) 254
XspI CTAG 2 cut(s) 89, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.