MD00G1028500.v1.1

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013443
Physical Location & Seq
Forward (+)
4667678 .. 4668328
651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1028500.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGACGGAGAAGAAGATCAAGCCAAACAATCTTCCGGGAGGATCCCTTTCACCCAACTCATCCAAGTGAGCGCTGATTTCATTCTTGCCCTGGCTATGCAAGAGCAAGAGCAGGCCTACACCATGCTCGAAACCATAGAAAGCGATAGTGAAGAAGACGACACTGAAATTGACTATGCTTCTTCGTCCTCTAGTGAAAACTATGACCCAGACGTTGCTGCTTTCCTTGAAAGCCGAGAATTTGATGCTGATGATTTCAGGTTTCTTGAGGACGAAGAAATTGGCAGCAGCAGTAGCAGCAGTGATCAAGAAACGGATGGTTTGGACGTGGATGAACTAACTTATGAGGAATTTTTAGCACTTGGAGAATTCATAGGGGAAGAAAAAAGAGGACTTCCCAGGAGTGAGATTTCTGCATGTTTGCATCCCTACACGTGCGAATTAGCGGTCGGTCAGAGCAAGACTAGCATCGATCGATGCGTGGTTTGTCAGCTCGAGTATGACGATGGTGAATCACTAGCTGCATTGTCCTGTGAACACCCTTATCATTGGGAGTGTATAAGCCAGTGGCTTCAGATTAAAAAGAGTTGCCCTATTTGCAGCACACAAGTTTCATCACCATCCTCATCGTCCAAGACCATGATTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

217

Amino Acids

24.06

Weight (kDa)

4.05

Isoelectric Point (pI)

70.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 158 - 200 6.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 159 - 200 5.7e-13 Ring finger domain
zf-C3HC4_2 PF13923 159 - 200 9.8e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 649
AciI CCGC 1 cut(s) 446
AclWI GGATC 2 cut(s) 37, 50
AcsI RAATTY 3 cut(s) 239, 350, 368
AcuI CTGAAG 1 cut(s) 557
AcvI CACGTG 1 cut(s) 435
AfeI AGCGCT 1 cut(s) 73
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 1 cut(s) 230
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 2 cut(s) 90, 398
AluBI AGCT 2 cut(s) 493, 521
AluI AGCT 2 cut(s) 493, 521
AlwI GGATC 2 cut(s) 37, 50
Ama87I CYCGRG 1 cut(s) 494
Aor51HI AGCGCT 1 cut(s) 73
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 6 cut(s) 218, 285, 288, 297, 521, 600
ApoI RAATTY 3 cut(s) 239, 350, 368
ArsI GACNNNNNNTTYG 2 cut(s) 432, 464
AspLEI GCGC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 3 cut(s) 43, 521, 609
AvaI CYCGRG 1 cut(s) 494
BamHI GGATCC 1 cut(s) 42
BbrPI CACGTG 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 6 cut(s) 205, 297, 300, 309, 508, 612
BccI CCATC 3 cut(s) 311, 500, 628
BciT130I CCWGG 2 cut(s) 92, 400
BclI TGATCA 1 cut(s) 304
BcnI CCSGG 1 cut(s) 37
BfaI CTAG 3 cut(s) 192, 465, 518
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 6 cut(s) 219, 286, 289, 298, 522, 601
BlsI GCNGC 6 cut(s) 220, 287, 290, 299, 523, 602
Bme1390I CCNGG 3 cut(s) 37, 92, 400
BmeT110I CYCGRG 1 cut(s) 494
BmgBI CACGTC 1 cut(s) 328
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 3 cut(s) 37, 92, 400
BmsI GCATC 4 cut(s) 235, 433, 467, 477
BpiI GAAGAC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 37
Bsa29I ATCGAT 2 cut(s) 471, 475
BsaAI YACGTR 1 cut(s) 435
BsaJI CCNNGG 2 cut(s) 90, 398
Bse1I ACTGG 1 cut(s) 565
BseBI CCWGG 2 cut(s) 92, 400
BseCI ATCGAT 2 cut(s) 471, 475
BseDI CCNNGG 2 cut(s) 90, 398
BseGI GGATG 5 cut(s) 60, 322, 337, 424, 620
BseNI ACTGG 1 cut(s) 565
BseXI GCAGC 6 cut(s) 205, 297, 300, 309, 508, 612
Bsh1285I CGRYCG 2 cut(s) 450, 475
BshFI GGCC 1 cut(s) 116
BshVI ATCGAT 2 cut(s) 471, 475
BsiEI CGRYCG 2 cut(s) 450, 475
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 1 cut(s) 36
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 494
Bsp143I GATC 4 cut(s) 16, 42, 304, 472
BspACI CCGC 1 cut(s) 446
BspANI GGCC 1 cut(s) 116
BspDI ATCGAT 2 cut(s) 471, 475
BspLI GGNNCC 1 cut(s) 44
BspPI GGATC 2 cut(s) 37, 50
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 2 cut(s) 90, 398
BssMI GATC 4 cut(s) 16, 42, 304, 472
Bst2UI CCWGG 2 cut(s) 92, 400
BstBAI YACGTR 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 114
BstF5I GGATG 5 cut(s) 60, 322, 337, 424, 620
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 4 cut(s) 19, 45, 307, 475
BstMBI GATC 4 cut(s) 16, 42, 304, 472
BstMCI CGRYCG 2 cut(s) 450, 475
BstMWI GCNNNNNNNGC 4 cut(s) 294, 297, 465, 597
BstNI CCWGG 2 cut(s) 92, 400
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 3 cut(s) 35, 90, 398
BstV1I GCAGC 6 cut(s) 205, 297, 300, 309, 508, 612
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
Bsu15I ATCGAT 2 cut(s) 471, 475
BsuRI GGCC 1 cut(s) 116
BsuTUI ATCGAT 2 cut(s) 471, 475
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 5 cut(s) 60, 322, 337, 424, 620
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 3 cut(s) 162, 307, 572
Cac8I GCNNGC 1 cut(s) 114
CfoI GCGC 1 cut(s) 74
ClaI ATCGAT 2 cut(s) 471, 475
CviAII CATG 3 cut(s) 124, 417, 640
CviJI RGCY 8 cut(s) 23, 95, 116, 234, 493, 521, 564, 571
CviKI_1 RGCY 8 cut(s) 23, 95, 116, 234, 493, 521, 564, 571
DpnI GATC 4 cut(s) 18, 44, 306, 474
DpnII GATC 4 cut(s) 16, 42, 304, 472
Eco147I AGGCCT 1 cut(s) 116
Eco47III AGCGCT 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 557
Eco72I CACGTG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 90, 398
FaeI CATG 3 cut(s) 127, 420, 643
FatI CATG 3 cut(s) 123, 416, 639
FbaI TGATCA 1 cut(s) 304
Fnu4HI GCNGC 6 cut(s) 219, 286, 289, 298, 522, 601
FokI GGATG 5 cut(s) 47, 329, 344, 411, 607
Fsp4HI GCNGC 6 cut(s) 219, 286, 289, 298, 522, 601
FspBI CTAG 3 cut(s) 192, 465, 518
GlaI GCGC 1 cut(s) 73
GluI GCNGC 6 cut(s) 219, 286, 289, 298, 522, 601
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 116
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 74
Hin1II CATG 3 cut(s) 127, 420, 643
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 1 cut(s) 512
HpaII CCGG 1 cut(s) 36
HphI GGTGA 3 cut(s) 43, 521, 609
Hpy166II GTNNAC 1 cut(s) 536
Hpy188I TCNGA 2 cut(s) 456, 576
Hpy188III TCNNGA 2 cut(s) 266, 308
Hpy8I GTNNAC 1 cut(s) 536
HpyCH4IV ACGT 3 cut(s) 213, 327, 434
HpyCH4V TGCA 5 cut(s) 100, 416, 424, 524, 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 294, 297, 465, 597
HpySE526I ACGT 3 cut(s) 213, 327, 434
Hsp92II CATG 3 cut(s) 127, 420, 643
HspAI GCGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 4 cut(s) 16, 42, 304, 472
Lsp1109I GCAGC 6 cut(s) 205, 297, 300, 309, 508, 612
LweI GCATC 4 cut(s) 235, 433, 467, 477
MaeI CTAG 3 cut(s) 192, 465, 518
MaeII ACGT 3 cut(s) 213, 327, 434
MalI GATC 4 cut(s) 18, 44, 306, 474
MboI GATC 4 cut(s) 16, 42, 304, 472
MboII GAAGA 8 cut(s) 23, 24, 26, 164, 167, 174, 287, 392
MflI RGATCY 1 cut(s) 42
MluCI AATT 6 cut(s) 168, 239, 279, 350, 368, 440
MnlI CCTC 6 cut(s) 33, 199, 262, 340, 383, 634
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 1 cut(s) 65
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 3 cut(s) 37, 92, 400
MvaI CCWGG 2 cut(s) 92, 400
MwoI GCNNNNNNNGC 4 cut(s) 294, 297, 465, 597
NciI CCSGG 1 cut(s) 37
NdeII GATC 4 cut(s) 16, 42, 304, 472
NlaIII CATG 3 cut(s) 127, 420, 643
NlaIV GGNNCC 1 cut(s) 44
NmeAIII GCCGAG 1 cut(s) 260
NspI RCATGY 1 cut(s) 420
PaeR7I CTCGAG 1 cut(s) 494
PceI AGGCCT 1 cut(s) 116
PcsI WCGNNNNNNNCGW 2 cut(s) 477, 501
PfeI GAWTC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 35
PkrI GCNGC 6 cut(s) 220, 287, 290, 299, 523, 602
Ple19I CGATCG 1 cut(s) 475
PmaCI CACGTG 1 cut(s) 435
PmlI CACGTG 1 cut(s) 435
Ppu21I YACGTR 1 cut(s) 435
PsiI TTATAA 1 cut(s) 649
Psp6I CCWGG 2 cut(s) 90, 398
PspCI CACGTG 1 cut(s) 435
PspGI CCWGG 2 cut(s) 90, 398
PspN4I GGNNCC 1 cut(s) 44
PspXI VCTCGAGB 1 cut(s) 494
PsuI RGATCY 1 cut(s) 42
PvuI CGATCG 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 6 cut(s) 219, 286, 289, 298, 522, 601
Sau3AI GATC 4 cut(s) 16, 42, 304, 472
ScrFI CCNGG 3 cut(s) 37, 92, 400
SetI ASST 6 cut(s) 216, 263, 330, 437, 495, 523
SfaNI GCATC 4 cut(s) 235, 433, 467, 477
Sfr274I CTCGAG 1 cut(s) 494
SlaI CTCGAG 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 2 cut(s) 266, 494
SmoI CTYRAG 2 cut(s) 266, 494
Sse9I AATT 6 cut(s) 168, 239, 279, 350, 368, 440
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 1 cut(s) 446
SspMI CTAG 3 cut(s) 192, 465, 518
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 3 cut(s) 35, 90, 398
TaiI ACGT 3 cut(s) 216, 330, 437
TaqI TCGA 4 cut(s) 129, 471, 475, 495
TaqII GACCGA 1 cut(s) 440
TasI AATT 6 cut(s) 168, 239, 279, 350, 368, 440
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 3 cut(s) 169, 307, 572
TseI GCWGC 6 cut(s) 218, 285, 288, 297, 521, 600
TspDTI ATGAA 4 cut(s) 70, 348, 361, 603
TspGWI ACGGA 2 cut(s) 21, 329
TspRI CASTG 3 cut(s) 169, 307, 572
XapI RAATTY 3 cut(s) 239, 350, 368
XceI RCATGY 1 cut(s) 420
XhoI CTCGAG 1 cut(s) 494
XspI CTAG 3 cut(s) 192, 465, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.