MD15G1344800.v1.1

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013443
Physical Location & Seq
Forward (+)
40371393 .. 40372046
654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1344800.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGACGGAGAAGAAGAACGAGCCAAACAATCTTCCCAGAGGACCCCTTTCACCGAACTCAACCAAGTGAGCGCGGATTTCATTCTAGCCATGGCTATGCAAGAGCAAGAGCAGGCCTACACAATGCTCGAAACCATAGAAAGCGATAGTGAAGAAGACGATGCTGAAATTGATGATGCTTCTTCGTCCTCTAATGAAAACTATGATCCAGACAATGCTGCTTTCTCCCAAAGCAGAGAATTTGATGCTGGTGATTTCAGGTTTCTTGAGGACGAAGAAATTGGCAGCAGCAGCAGCAGCAGTAGCGACCAAGAAATGGATGATTTGGACGTGGATGAACTAACTTATGAGGAATTGTTAGCACTTGGAGAGTTCATAGGGGAAGAAAAAAGAGGACTTCCCAGCAGTGAGATTTCTACATGTTTGCATCCCTACACCTGCAAATTAGCGGTCGATCAGAGCGAGACTAGCATCGATCGATGCGTGGTTTGTCAGATCGAATATGAAGATGGTGAATCATTAGCTGCATTGTCCTGTGAACACCCTTATCATTGGGAGTGTATAAGCCAATGGCTTCAGATTAAAAAGAGTTGCCCTATTTGCAGCACAGAAGTTTCATCACCATCCTCATCATCCAAGACCAAGAATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

218

Amino Acids

24.23

Weight (kDa)

4.05

Isoelectric Point (pI)

73.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 159 - 201 1.2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 160 - 201 1.7e-12 Ring finger domain
zf-C3HC4_2 PF13923 160 - 201 1.4e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 652
AarI CACCTGC 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 446
AccB7I CCANNNNNTGG 1 cut(s) 316
AccII CGCG 1 cut(s) 74
AciI CCGC 2 cut(s) 74, 449
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 2 cut(s) 239, 646
AcuI CTGAAG 1 cut(s) 560
AfiI CCNNNNNNNGG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 419
AjiI CACGTC 1 cut(s) 331
AluBI AGCT 1 cut(s) 524
AluI AGCT 1 cut(s) 524
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 1 cut(s) 200
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 8 cut(s) 218, 285, 288, 291, 294, 297, 524, 603
ApoI RAATTY 2 cut(s) 239, 646
ArsI GACNNNNNNTTYG 2 cut(s) 435, 467
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 42
AsuHPI GGTGA 4 cut(s) 43, 263, 524, 612
AvaII GGWCC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 8 cut(s) 205, 297, 300, 303, 306, 309, 511, 615
BccI CCATC 2 cut(s) 503, 631
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 2 cut(s) 86, 468
BfuAI ACCTGC 1 cut(s) 446
BisI GCNGC 8 cut(s) 219, 286, 289, 292, 295, 298, 525, 604
BlsI GCNGC 8 cut(s) 220, 287, 290, 293, 296, 299, 526, 605
Bme18I GGWCC 1 cut(s) 42
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 44
BmsI GCATC 6 cut(s) 151, 166, 235, 436, 470, 480
BpiI GAAGAC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 287
Bsa29I ATCGAT 2 cut(s) 474, 478
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 316
BseCI ATCGAT 2 cut(s) 474, 478
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 5 cut(s) 325, 340, 427, 623, 632
BseLI CCNNNNNNNGG 1 cut(s) 316
BseXI GCAGC 8 cut(s) 205, 297, 300, 303, 306, 309, 511, 615
BseYI CCCAGC 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 2 cut(s) 453, 478
BshFI GGCC 1 cut(s) 116
BshVI ATCGAT 2 cut(s) 474, 478
BsiEI CGRYCG 2 cut(s) 453, 478
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 458
BsnI GGCC 1 cut(s) 116
Bsp143I GATC 4 cut(s) 205, 454, 475, 495
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 2 cut(s) 74, 449
BspANI GGCC 1 cut(s) 116
BspDI ATCGAT 2 cut(s) 474, 478
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 44
BspMI ACCTGC 1 cut(s) 446
BspPI GGATC 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 4 cut(s) 205, 454, 475, 495
BssT1I CCWWGG 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 5 cut(s) 325, 340, 427, 623, 632
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 4 cut(s) 208, 457, 478, 498
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 4 cut(s) 205, 454, 475, 495
BstMCI CGRYCG 2 cut(s) 453, 478
BstMWI GCNNNNNNNGC 6 cut(s) 291, 294, 297, 303, 468, 600
BstNSI RCATGY 1 cut(s) 423
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 8 cut(s) 205, 297, 300, 303, 306, 309, 511, 615
BstV2I GAAGAC 1 cut(s) 162
Bsu15I ATCGAT 2 cut(s) 474, 478
BsuRI GGCC 1 cut(s) 116
BsuTUI ATCGAT 2 cut(s) 474, 478
BtgI CCRYGG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 5 cut(s) 325, 340, 427, 623, 632
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 412
BveI ACCTGC 1 cut(s) 446
Cac8I GCNNGC 1 cut(s) 114
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 42
ClaI ATCGAT 2 cut(s) 474, 478
CviAII CATG 2 cut(s) 91, 420
CviJI RGCY 7 cut(s) 23, 89, 95, 116, 524, 567, 574
CviKI_1 RGCY 7 cut(s) 23, 89, 95, 116, 524, 567, 574
DpnI GATC 4 cut(s) 207, 456, 477, 497
DpnII GATC 4 cut(s) 205, 454, 475, 495
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 116
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 94, 423
FatI CATG 2 cut(s) 90, 419
Fnu4HI GCNGC 8 cut(s) 219, 286, 289, 292, 295, 298, 525, 604
FokI GGATG 5 cut(s) 332, 347, 414, 610, 619
Fsp4HI GCNGC 8 cut(s) 219, 286, 289, 292, 295, 298, 525, 604
FspBI CTAG 2 cut(s) 86, 468
GlaI GCGC 1 cut(s) 73
GluI GCNGC 8 cut(s) 219, 286, 289, 292, 295, 298, 525, 604
GsaI CCCAGC 1 cut(s) 405
HaeIII GGCC 1 cut(s) 116
HhaI GCGC 1 cut(s) 74
Hin1II CATG 2 cut(s) 94, 423
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 1 cut(s) 515
HphI GGTGA 4 cut(s) 43, 263, 524, 612
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 3 cut(s) 459, 495, 579
Hpy188III TCNNGA 2 cut(s) 209, 266
Hpy8I GTNNAC 1 cut(s) 539
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 5 cut(s) 100, 427, 441, 527, 603
HpyF10VI GCNNNNNNNGC 6 cut(s) 291, 294, 297, 303, 468, 600
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 94, 423
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 4 cut(s) 205, 454, 475, 495
LpnPI CCDG 8 cut(s) 50, 98, 222, 234, 244, 415, 451, 547
Lsp1109I GCAGC 8 cut(s) 205, 297, 300, 303, 306, 309, 511, 615
LweI GCATC 6 cut(s) 151, 166, 235, 436, 470, 480
MaeI CTAG 2 cut(s) 86, 468
MaeII ACGT 1 cut(s) 330
MalI GATC 4 cut(s) 207, 456, 477, 497
MboI GATC 4 cut(s) 205, 454, 475, 495
MboII GAAGA 9 cut(s) 23, 24, 26, 164, 167, 174, 287, 395, 518
MluCI AATT 6 cut(s) 168, 239, 279, 353, 443, 646
MnlI CCTC 6 cut(s) 33, 199, 262, 343, 386, 637
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 1 cut(s) 95
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 6 cut(s) 291, 294, 297, 303, 468, 600
NcoI CCATGG 1 cut(s) 90
NdeII GATC 4 cut(s) 205, 454, 475, 495
NlaIII CATG 2 cut(s) 94, 423
NlaIV GGNNCC 1 cut(s) 44
NspI RCATGY 1 cut(s) 423
PaqCI CACCTGC 1 cut(s) 446
PceI AGGCCT 1 cut(s) 116
PciI ACATGT 1 cut(s) 419
PcsI WCGNNNNNNNCGW 2 cut(s) 459, 480
PfeI GAWTC 1 cut(s) 515
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 8 cut(s) 220, 287, 290, 293, 296, 299, 526, 605
Ple19I CGATCG 1 cut(s) 478
PpuMI RGGWCCY 1 cut(s) 42
PscI ACATGT 1 cut(s) 419
PsiI TTATAA 1 cut(s) 652
Psp5II RGGWCCY 1 cut(s) 42
PspFI CCCAGC 1 cut(s) 401
PspN4I GGNNCC 1 cut(s) 44
PspPI GGNCC 1 cut(s) 42
PspPPI RGGWCCY 1 cut(s) 42
PvuI CGATCG 1 cut(s) 478
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 8 cut(s) 219, 286, 289, 292, 295, 298, 525, 604
Sau3AI GATC 4 cut(s) 205, 454, 475, 495
Sau96I GGNCC 1 cut(s) 42
SetI ASST 4 cut(s) 263, 333, 440, 526
SfaNI GCATC 6 cut(s) 151, 166, 235, 436, 470, 480
SinI GGWCC 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 6 cut(s) 168, 239, 279, 353, 443, 646
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 2 cut(s) 74, 449
SspMI CTAG 2 cut(s) 86, 468
StuI AGGCCT 1 cut(s) 116
StyI CCWWGG 1 cut(s) 90
TaiI ACGT 1 cut(s) 333
TaqI TCGA 5 cut(s) 129, 453, 474, 478, 498
TasI AATT 6 cut(s) 168, 239, 279, 353, 443, 646
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 1 cut(s) 412
TseI GCWGC 8 cut(s) 218, 285, 288, 291, 294, 297, 524, 603
TspDTI ATGAA 6 cut(s) 70, 210, 351, 364, 519, 606
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 412
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 2 cut(s) 239, 646
XceI RCATGY 1 cut(s) 423
XspI CTAG 2 cut(s) 86, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.