Rh6CG385800

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0013443
Physical Location & Seq
Reverse (-)
57197825 .. 57198955
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG385800.1

Sequence Viewer

Length: 651 bp
ATGGAGGGAGAAGAAGAGAGCAAGCAATCAGATCCGCGGAGCTTAATCCCTTTCTCCGAGCTCAACCAAGTCAGCTCGGATTTCATCCTAGCCATGACTATGCACGAACAAGAGCACGCATACAGGATGCTCGAAACAATTGAAAGTGAAAGCGAAGAAGAAGATGATGATGATCAAATTCATGATGTCGACGATGGTACTTCTTCCTCTGAGGAATTCTATCATCAAGATGATGCTGATTTCTTTGATCTTCTCAGTGACGATAATGGAAGCAACAGTGACCAAGAAATGGAGGAAGATGACTTTGGTGATCTGGACGTGGATGAACTAACTTATGAGGAATTGTTAGCACTTGGAGAGTTCATAGGGGAAGAGAAAAGAGGTCTGCCCAGCAATGAAATTTCTACATGTTTGCATCCCCACACATACAAATTATCTCCAATTTATCAGAGCAAGAATAGCATTATTGATAAGTGTGTTGTATGTCAGTATGAGTATGAAGATGGTGAGGCTTTGGCTGCATTGCCTTGTGAACACCATTATCATTTGGAGTGTATAAGCAACTGGCTTCAGATTAAAAAGTGTTGCCCTATTTGCAGCACAGAAGTTTCATCATCATCGTCATCATCATCCAAAACCAAAATTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

216

Amino Acids

24.53

Weight (kDa)

4.08

Isoelectric Point (pI)

62.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 152 - 199 5.6e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 157 - 199 1.2e-12 Ring finger domain
zf-C3HC4_2 PF13923 158 - 199 3.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 159 - 186 1.2e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 1 cut(s) 189
AccII CGCG 1 cut(s) 37
AciI CCGC 2 cut(s) 35, 37
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 3 cut(s) 177, 215, 399
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 1 cut(s) 407
AgsI TTSAA 1 cut(s) 143
AjiI CACGTC 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AluBI AGCT 3 cut(s) 42, 61, 75
AluI AGCT 3 cut(s) 42, 61, 75
Alw21I GWGCWC 2 cut(s) 63, 117
AlwI GGATC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 518, 597
ApoI RAATTY 3 cut(s) 177, 215, 399
AsuHPI GGTGA 2 cut(s) 320, 518
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 2 cut(s) 63, 117
BbvI GCAGC 2 cut(s) 505, 609
BccI CCATC 2 cut(s) 188, 497
BclI TGATCA 1 cut(s) 172
BfaI CTAG 1 cut(s) 89
BisI GCNGC 2 cut(s) 519, 598
BlsI GCNGC 2 cut(s) 520, 599
BmgBI CACGTC 1 cut(s) 319
BmsI GCATC 3 cut(s) 117, 223, 424
BsaBI GATNNNNATC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 35
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 569
Bse3DI GCAATG 2 cut(s) 400, 521
Bse8I GATNNNNATC 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 35
BseGI GGATG 5 cut(s) 84, 132, 328, 415, 629
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 2 cut(s) 400, 521
BseMII CTCAG 2 cut(s) 201, 268
BseNI ACTGG 1 cut(s) 569
BseXI GCAGC 2 cut(s) 505, 609
BseYI CCCAGC 1 cut(s) 389
Bsh1236I CGCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 63, 117
BslI CCNNNNNNNGG 1 cut(s) 289
Bsp1286I GDGCHC 2 cut(s) 63, 117
Bsp143I GATC 4 cut(s) 31, 172, 247, 310
BspACI CCGC 2 cut(s) 35, 37
BspCNI CTCAG 2 cut(s) 202, 267
BspFNI CGCG 1 cut(s) 37
BspHI TCATGA 1 cut(s) 181
BspPI GGATC 1 cut(s) 26
BsrDI GCAATG 2 cut(s) 400, 521
BsrI ACTGG 1 cut(s) 569
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 4 cut(s) 31, 172, 247, 310
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 2 cut(s) 9, 366
BstC8I GCNNGC 2 cut(s) 23, 117
BstDEI CTNAG 2 cut(s) 210, 254
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 5 cut(s) 84, 132, 328, 415, 629
BstFNI CGCG 1 cut(s) 37
BstKTI GATC 4 cut(s) 34, 175, 250, 313
BstMBI GATC 4 cut(s) 31, 172, 247, 310
BstMWI GCNNNNNNNGC 3 cut(s) 459, 518, 594
BstNSI RCATGY 1 cut(s) 411
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 505, 609
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BtgI CCRYGG 1 cut(s) 35
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 5 cut(s) 84, 132, 328, 415, 629
BtsIMutI CAGTG 2 cut(s) 262, 283
Cac8I GCNNGC 2 cut(s) 23, 117
CciI TCATGA 1 cut(s) 181
Cfr42I CCGCGG 1 cut(s) 38
Csp6I GTAC 1 cut(s) 198
CviAII CATG 3 cut(s) 94, 182, 408
CviJI RGCY 7 cut(s) 42, 61, 75, 92, 512, 518, 568
CviKI_1 RGCY 7 cut(s) 42, 61, 75, 92, 512, 518, 568
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 2 cut(s) 210, 254
DpnI GATC 4 cut(s) 33, 174, 249, 312
DpnII GATC 4 cut(s) 31, 172, 247, 310
Eam1104I CTCTTC 2 cut(s) 9, 366
EarI CTCTTC 2 cut(s) 9, 366
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 554
EcoICRI GAGCTC 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 215
EcoT38I GRGCYC 1 cut(s) 63
FaeI CATG 3 cut(s) 97, 185, 411
FatI CATG 3 cut(s) 93, 181, 407
FbaI TGATCA 1 cut(s) 172
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 2 cut(s) 519, 598
FokI GGATG 5 cut(s) 71, 139, 335, 402, 616
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 2 cut(s) 519, 598
FspBI CTAG 1 cut(s) 89
GluI GCNGC 2 cut(s) 519, 598
GsaI CCCAGC 1 cut(s) 393
Hin1II CATG 3 cut(s) 97, 185, 411
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HphI GGTGA 2 cut(s) 320, 518
Hpy166II GTNNAC 2 cut(s) 190, 533
Hpy188I TCNGA 6 cut(s) 31, 58, 79, 211, 450, 573
Hpy188III TCNNGA 3 cut(s) 182, 227, 314
Hpy8I GTNNAC 2 cut(s) 190, 533
Hpy99I CGWCG 1 cut(s) 194
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 4 cut(s) 103, 415, 521, 597
HpyF10VI GCNNNNNNNGC 3 cut(s) 459, 518, 594
HpyF3I CTNAG 2 cut(s) 210, 254
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 3 cut(s) 97, 185, 411
Ksp22I TGATCA 1 cut(s) 172
KspI CCGCGG 1 cut(s) 38
Kzo9I GATC 4 cut(s) 31, 172, 247, 310
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 4 cut(s) 109, 299, 403, 550
Lsp1109I GCAGC 2 cut(s) 505, 609
LweI GCATC 3 cut(s) 117, 223, 424
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 2 cut(s) 257, 278
MalI GATC 4 cut(s) 33, 174, 249, 312
MboI GATC 4 cut(s) 31, 172, 247, 310
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 63, 117
MluCI AATT 8 cut(s) 138, 177, 215, 341, 399, 431, 441, 642
MnlI CCTC 6 cut(s) 205, 217, 286, 331, 374, 502
MseI TTAA 2 cut(s) 44, 576
MslI CAYNNNNRTG 2 cut(s) 98, 228
MspA1I CMGCKG 1 cut(s) 37
MunI CAATTG 1 cut(s) 138
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 3 cut(s) 459, 518, 594
NdeII GATC 4 cut(s) 31, 172, 247, 310
NlaIII CATG 3 cut(s) 97, 185, 411
NmuCI GTSAC 2 cut(s) 257, 278
NspI RCATGY 1 cut(s) 411
PagI TCATGA 1 cut(s) 181
PciI ACATGT 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 2 cut(s) 520, 599
PscI ACATGT 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 389
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
RseI CAYNNNNRTG 2 cut(s) 98, 228
SacI GAGCTC 1 cut(s) 63
SacII CCGCGG 1 cut(s) 38
SalI GTCGAC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 44, 576
SatI GCNGC 2 cut(s) 519, 598
Sau3AI GATC 4 cut(s) 31, 172, 247, 310
SduI GDGCHC 2 cut(s) 63, 117
SetI ASST 5 cut(s) 44, 63, 77, 321, 385
SfaNI GCATC 3 cut(s) 117, 223, 424
Sfr303I CCGCGG 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 38
SmiMI CAYNNNNRTG 2 cut(s) 98, 228
Sse9I AATT 8 cut(s) 138, 177, 215, 341, 399, 431, 441, 642
SsiI CCGC 2 cut(s) 35, 37
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 1 cut(s) 63
TaaI ACNGT 1 cut(s) 278
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 132, 189
TasI AATT 8 cut(s) 138, 177, 215, 341, 399, 431, 441, 642
Tru1I TTAA 2 cut(s) 44, 576
Tru9I TTAA 2 cut(s) 44, 576
TscAI CASTG 2 cut(s) 262, 283
TseFI GTSAC 2 cut(s) 257, 278
TseI GCWGC 2 cut(s) 518, 597
Tsp45I GTSAC 2 cut(s) 257, 278
TspDTI ATGAA 7 cut(s) 73, 170, 339, 352, 411, 513, 600
TspRI CASTG 2 cut(s) 262, 283
Van91I CCANNNNNTGG 1 cut(s) 289
XapI RAATTY 3 cut(s) 177, 215, 399
XceI RCATGY 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.