pycom15g30940

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013443
Physical Location & Seq
Forward (+)
28950982 .. 28951629
648 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g30940.1

Sequence Viewer

Length: 648 bp
ATGGACGGAGAAGAAGAACGAGCCAAACAATCTTCCCGGAGGACCCCTTTCACCGAACTCAACCAAGTGAGCGCAGATTTCATTCTAGCCATGGCTATGCAAGAGCAAGAGCAGGCCTACACAATGCTCGAAACCATAGAAAGCGATAGTGAAGAAGACGATGCTGAAATTGATGATGCTTCTTCGTCCTCTAATGAAAACTATGATCCAGACAACGCTGATTTCTCCGAAAGCAGACAATTTGATGCTGGTGATTTCAGGTTTTTTGAGGACGAAGAAATTGGCAGCAGCAGCAGCAGCGACCAAGAAATGGATGATTTGGACGTGGATGAACTAACTTATGAGGAATTGTTAGCACTTGGAGAGTTCATAGGGGAAGAAAAAAGAGGACTTCCCAGCAGTGAGATTTCTACATGTTTGCATCCCTACACCTGCAAATTAGCGGTCGATCAGAGCAAGATTAGCATCGATCGGTGTGTGGTTTGTCAGATCGAATATGAAGATGGTGAATTACTAGCAGCATTGTCCTGTGAACACCCTTATCATTGGGAGTGTATAAGCCAATGGCTTCAGATTAAAAAGAGTTGTCCTATTTGCAGCACAGAAGTTTCATCACCATCCTCATCATCCAAGACCAAGAATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

216

Amino Acids

24.2

Weight (kDa)

4.05

Isoelectric Point (pI)

71.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 157 - 199 1.3e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 158 - 199 9.7e-12 Ring finger domain
zf-C3HC4_2 PF13923 158 - 199 1.2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013443)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47180
fragaria_vesca FvH4_2g27450
malus_domestica MD00G1028500.v1.1 MD15G1344800.v1.1
prunus_persica Prupe.1G493000_v2.0.a1
pyrus_communis pycom08g13690 pycom15g30940
rosa_chinensis RchiOBHm_Chr6g0296291
rosa_laevigata RLG00000011709
rosa_multiflora Rmu_sc0004708.1_g000007
rosa_roxburghii Rroxscaffold_7G00171660 Rroxscaffold_7G00178410
rosa_rugosa Rorug06G0260700
rosa_samantha Rh6AG372400 Rh6BG380400 Rh6CG385800 Rh6DG373300
rosa_wichuraiana Rw6G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 646
AarI CACCTGC 1 cut(s) 440
Acc36I ACCTGC 1 cut(s) 440
AccB7I CCANNNNNTGG 1 cut(s) 310
AciI CCGC 1 cut(s) 443
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 1 cut(s) 640
AcuI CTGAAG 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 310
AflIII ACRYGT 1 cut(s) 413
AjiI CACGTC 1 cut(s) 325
AlwI GGATC 1 cut(s) 200
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 7 cut(s) 285, 288, 291, 294, 297, 518, 597
ApoI RAATTY 1 cut(s) 640
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 42
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 4 cut(s) 43, 263, 518, 606
AvaII GGWCC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 7 cut(s) 297, 300, 303, 306, 309, 530, 609
BccI CCATC 2 cut(s) 497, 625
BcnI CCSGG 1 cut(s) 37
BfaI CTAG 2 cut(s) 86, 515
BfuAI ACCTGC 1 cut(s) 440
BisI GCNGC 7 cut(s) 286, 289, 292, 295, 298, 519, 598
BlsI GCNGC 7 cut(s) 287, 290, 293, 296, 299, 520, 599
Bme1390I CCNGG 1 cut(s) 37
Bme18I GGWCC 1 cut(s) 42
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 1 cut(s) 37
BmsI GCATC 5 cut(s) 151, 166, 235, 430, 474
BpiI GAAGAC 1 cut(s) 162
BpuMI CCSGG 1 cut(s) 37
Bsa29I ATCGAT 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse8I GATNNNNATC 1 cut(s) 464
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 5 cut(s) 319, 334, 421, 617, 626
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 1 cut(s) 310
BseXI GCAGC 7 cut(s) 297, 300, 303, 306, 309, 530, 609
BseYI CCCAGC 1 cut(s) 395
Bsh1285I CGRYCG 2 cut(s) 447, 472
BshFI GGCC 1 cut(s) 116
BshVI ATCGAT 1 cut(s) 468
BsiEI CGRYCG 2 cut(s) 447, 472
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 1 cut(s) 310
BsnI GGCC 1 cut(s) 116
Bsp143I GATC 4 cut(s) 205, 448, 469, 489
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 1 cut(s) 443
BspANI GGCC 1 cut(s) 116
BspDI ATCGAT 1 cut(s) 468
BspLI GGNNCC 1 cut(s) 44
BspMI ACCTGC 1 cut(s) 440
BspPI GGATC 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 4 cut(s) 205, 448, 469, 489
BssT1I CCWWGG 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 5 cut(s) 319, 334, 421, 617, 626
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 4 cut(s) 208, 451, 472, 492
BstMBI GATC 4 cut(s) 205, 448, 469, 489
BstMCI CGRYCG 2 cut(s) 447, 472
BstMWI GCNNNNNNNGC 4 cut(s) 291, 294, 297, 462
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 1 cut(s) 35
BstV1I GCAGC 7 cut(s) 297, 300, 303, 306, 309, 530, 609
BstV2I GAAGAC 1 cut(s) 162
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 1 cut(s) 116
BsuTUI ATCGAT 1 cut(s) 468
BtgI CCRYGG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 5 cut(s) 319, 334, 421, 617, 626
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
BveI ACCTGC 1 cut(s) 440
Cac8I GCNNGC 1 cut(s) 114
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 42
ClaI ATCGAT 1 cut(s) 468
CviAII CATG 2 cut(s) 91, 414
CviJI RGCY 6 cut(s) 23, 89, 95, 116, 561, 568
CviKI_1 RGCY 6 cut(s) 23, 89, 95, 116, 561, 568
DpnI GATC 4 cut(s) 207, 450, 471, 491
DpnII GATC 4 cut(s) 205, 448, 469, 489
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 116
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 94, 417
FatI CATG 2 cut(s) 90, 413
Fnu4HI GCNGC 7 cut(s) 286, 289, 292, 295, 298, 519, 598
FokI GGATG 5 cut(s) 326, 341, 408, 604, 613
Fsp4HI GCNGC 7 cut(s) 286, 289, 292, 295, 298, 519, 598
FspBI CTAG 2 cut(s) 86, 515
GlaI GCGC 1 cut(s) 73
GluI GCNGC 7 cut(s) 286, 289, 292, 295, 298, 519, 598
GsaI CCCAGC 1 cut(s) 399
HaeIII GGCC 1 cut(s) 116
HapII CCGG 1 cut(s) 37
HhaI GCGC 1 cut(s) 74
Hin1II CATG 2 cut(s) 94, 417
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HpaII CCGG 1 cut(s) 37
HphI GGTGA 4 cut(s) 43, 263, 518, 606
Hpy166II GTNNAC 1 cut(s) 533
Hpy188I TCNGA 4 cut(s) 229, 453, 489, 573
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 1 cut(s) 533
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 4 cut(s) 100, 421, 435, 597
HpyF10VI GCNNNNNNNGC 4 cut(s) 291, 294, 297, 462
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 2 cut(s) 94, 417
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 4 cut(s) 205, 448, 469, 489
LpnPI CCDG 8 cut(s) 50, 98, 222, 234, 244, 409, 445, 541
Lsp1109I GCAGC 7 cut(s) 297, 300, 303, 306, 309, 530, 609
LweI GCATC 5 cut(s) 151, 166, 235, 430, 474
MaeI CTAG 2 cut(s) 86, 515
MaeII ACGT 1 cut(s) 324
MalI GATC 4 cut(s) 207, 450, 471, 491
MboI GATC 4 cut(s) 205, 448, 469, 489
MboII GAAGA 9 cut(s) 23, 24, 26, 164, 167, 174, 287, 389, 512
MluCI AATT 7 cut(s) 168, 239, 279, 347, 437, 509, 640
MnlI CCTC 6 cut(s) 33, 199, 262, 337, 380, 631
MseI TTAA 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 95
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 37
MwoI GCNNNNNNNGC 4 cut(s) 291, 294, 297, 462
NciI CCSGG 1 cut(s) 37
NcoI CCATGG 1 cut(s) 90
NdeII GATC 4 cut(s) 205, 448, 469, 489
NlaIII CATG 2 cut(s) 94, 417
NlaIV GGNNCC 1 cut(s) 44
NspI RCATGY 1 cut(s) 417
PaqCI CACCTGC 1 cut(s) 440
PceI AGGCCT 1 cut(s) 116
PciI ACATGT 1 cut(s) 413
PflMI CCANNNNNTGG 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 35
PkrI GCNGC 7 cut(s) 287, 290, 293, 296, 299, 520, 599
Ple19I CGATCG 1 cut(s) 472
PpuMI RGGWCCY 1 cut(s) 42
PscI ACATGT 1 cut(s) 413
PsiI TTATAA 1 cut(s) 646
Psp5II RGGWCCY 1 cut(s) 42
PspFI CCCAGC 1 cut(s) 395
PspN4I GGNNCC 1 cut(s) 44
PspPI GGNCC 1 cut(s) 42
PspPPI RGGWCCY 1 cut(s) 42
PvuI CGATCG 1 cut(s) 472
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 7 cut(s) 286, 289, 292, 295, 298, 519, 598
Sau3AI GATC 4 cut(s) 205, 448, 469, 489
Sau96I GGNCC 1 cut(s) 42
ScrFI CCNGG 1 cut(s) 37
SetI ASST 3 cut(s) 263, 327, 434
SfaNI GCATC 5 cut(s) 151, 166, 235, 430, 474
SinI GGWCC 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 7 cut(s) 168, 239, 279, 347, 437, 509, 640
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 1 cut(s) 443
SspMI CTAG 2 cut(s) 86, 515
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 1 cut(s) 35
StyI CCWWGG 1 cut(s) 90
TaiI ACGT 1 cut(s) 327
TaqI TCGA 4 cut(s) 129, 447, 468, 492
TasI AATT 7 cut(s) 168, 239, 279, 347, 437, 509, 640
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 1 cut(s) 406
TseI GCWGC 7 cut(s) 285, 288, 291, 294, 297, 518, 597
TspDTI ATGAA 6 cut(s) 70, 210, 345, 358, 513, 600
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 406
Van91I CCANNNNNTGG 1 cut(s) 310
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 1 cut(s) 640
XceI RCATGY 1 cut(s) 417
XspI CTAG 2 cut(s) 86, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.