Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009275

Physical Location & Seq

Genomic Coordinates Forward (+)

18620450 .. 18623251

Gene Structure 2802 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G50230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1983 bp

ATGACACTCTTCTTCTTCTACTCTCTTCTCTTCCTTCTCCTCCTCCTCCGTATCTCCACCGCCTCTCTCCCGGCGACGAACTACTTCGACTCCTTCTTACCATCCGACGCCGTCGCACTTCTCTCCTTCAAATCCACCGCTGATCTCGACAACAAGCTTCTATACTCTCTCACCGAGCCTTACGACTACTGTCAATGGCGCGGCGTCGATTGCTCTCAAGATCGCGTCGTTCGTCTTATCCTCGACGGTGTTGGTCTCCGGGGAAGTTTCTCACCGGAGACTCTTAGTCGTCTTGATCAGCTTCGTGTTCTTAGCCTTGAGAATAACTCAATCTCGGGTTCGATTCCTGATCTATCTCCTCTCGTCAATCTCAAAACCCTAACCCTAAGCAAGAATGGTTTCTCCGGTACTCTTTCTTCTTCAATTCTCTCTCTTCGTCGGTTAACAGAACTTGATCTATCGTTTAACAACTTCTCCGGTGAGATTCCGTCGGGGATCAATGCTCTGAGTCGACTCAGTTCTTTAAATCTCGAGTTTAATCGACTTAACGGAACTCTCCCGCCGTTAAATCTTTCTTCTCTGATTTCATTTAACGTTTCCAGTAACAACCTCACCGGTTTAGTTCCGTTAACAAAGACTCTGTTACGATTCAACGCGTCTTCGTTTTCGTCAAACCCTGGACTCTGCGGTGAGATCATTAACCGATCTTGCGGTTTACATTCTTCTTCTCCGTTCTTTGGTTCACCTAAACCAAACACCACGTCATCAACATCTTCTGCTTCTTCGTCTGAAGCACCGGTTATTCAAAGTGAACAGAACGGTGAAGCAGCGATGATTGTTCCACCGGTGGTTAAAAAGGTTAAAAACGGTTGGTTAGTTCTTGGTTTCACCATTGGTCTCGCTTCTTTGATAGTTCTAGGTCTCTGCCTCGTCGTGTTCTCGTTGTTTATAAAAAACCGGAGAGAAGACTACGATGACGTGATAATTACGCAACCTAAGCGAGAAGAAGAAAACAAAGAAATCAAGATCCAATTTCAAACCACTGCGCCGTCGTCGAAAAAGCGAATCCCAAGAAACGGAGATTTGATATTCTGCGGCGAAGGAGGAGGAGGAGGAGAAGCAATGTACACAGTTGACCAGTTGATGAGAGCTTCAGCAGAGCTTTTAGGAAGAGGATCAGTAGGTACAACGTATAAAGCGGTGATGGTTAATCAAATGATCGTGACGGTGAAGAGATTTGCTCCGTCGAAAACAGCGATTACTAGTGATTTAGAGTTTGAGAATCAGATGGAGATAGTTGGTGGACTTAAGCATCCGAATCTTGTTCCGGTTAAAGCTTATTTCCAGTCAAATGGAGAGAGACTTGTCATCTATGAGTACCAACCTAATGGAAGTCTCTTCAATCTTATTCATGGTTCGAGAACATCAAAGGCAAAGCCATTACATTGGACATCATGTTTGAAGATAGCAGAAGATGTAGCTCAAGCATTACATTACATTCACCAATCATCAGCCAAATTCCATGGAAACCTTAAGTCCACTAACATCCTCCTTGGTCACGACTTTGAAGCTTGTGTCACAGATTATTGTTTAAGTGTCTTAACAGACTCATCTGTTCCTCCCAATGACCCGGACATCTCTTCCTATAAAGCACCAGAGATAAGGAAATCAACAGATAGTCGTCCCACGTCGAAATGCGATGTTTACTCGTTCGGGGTCTTCCTCTTAGAGCTATTGACCGGTAAAACCGCTTCGAGGCAACCGATTATGGAGCCAAACGATATGTTGGATTGGGTAAGAGCAATGAGGCAAGAAGAAGAAAGGTCAAAGGAAGAGAATGGGCTAGAGATGATGACTCAAACGGCTTGTCTTTGCCGTGTAACATCGCCAGAGCAAAGGCCCACTATGAAAGAAGTGATCAAAATGATTCAAGAGATTAAGGGGAGTGTTGTGATGACTGAAGAGAACGAAAAATTCCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

660

Amino Acids

72.76

Weight (kDa)

6.5

Isoelectric Point (pI)

46.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	94 - 187	4.1e-07	Leucine-rich repeat region
Pkinase	PF00069	387 - 641	1.3e-30	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	387 - 642	2.9e-30	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50230 AT3G50230 AT5G67200
fragaria_vesca	FvH4_1g16410
malus_domestica	MD02G1176600.v1.1 MD08G1095300.v1.1
prunus_persica	Prupe.6G231100_v2.0.a1
pyrus_communis	pycom01g08530 pycom02g14290 pycom15g07270
rosa_chinensis	RchiOBHm_Chr2g0106291
rosa_laevigata	RLG00000017486
rosa_multiflora	Rmu_sc0020846.1_g000001 Rmu_sc0021309.1_g000001 Rmu_ssc0000338.1_g000005
rosa_roxburghii	Rroxscaffold_2G00137300
rosa_rugosa	Rorug02G0134500
rosa_samantha	Rh2AG185300 Rh2BG195600 Rh2CG190400 Rh2DG191500
rosa_wichuraiana	Rw2G014470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	950
AccI	GTMKAC	1 cut(s)	511
AccII	CGCG	3 cut(s)	201, 225, 656
AciI	CCGC	9 cut(s)	60, 138, 201, 560, 687, 711, 1095, 1199, 1749
AclI	AACGTT	1 cut(s)	594
AclWI	GGATC	3 cut(s)	503, 1021, 1183
AcsI	RAATTY	2 cut(s)	1517, 1973
AcuI	CTGAAG	3 cut(s)	810, 1137, 1980
AcyI	GRCGYC	2 cut(s)	108, 204
AfaI	GTAC	4 cut(s)	409, 1127, 1186, 1379
AfiI	CCNNNNNNNGG	3 cut(s)	737, 1076, 1755
AflII	CTTAAG	2 cut(s)	1307, 1532
AflIII	ACRYGT	1 cut(s)	654
AgeI	ACCGGT	4 cut(s)	614, 796, 844, 1739
AgsI	TTSAA	9 cut(s)	130, 423, 652, 806, 1037, 1402, 1462, 1568, 1931
AhdI	GACNNNNNGTC	1 cut(s)	285
AhlI	ACTAGT	1 cut(s)	1262
AjiI	CACGTC	3 cut(s)	762, 979, 1689
AjnI	CCWGG	1 cut(s)	676
AluBI	AGCT	8 cut(s)	157, 301, 1151, 1162, 1337, 1481, 1571, 1732
AluI	AGCT	8 cut(s)	157, 301, 1151, 1162, 1337, 1481, 1571, 1732
Alw26I	GTCTC	6 cut(s)	260, 272, 902, 926, 1354, 1400
AlwI	GGATC	3 cut(s)	503, 1021, 1183
Ama87I	CYCGRG	2 cut(s)	334, 530
AoxI	GGCC	1 cut(s)	1898
ApeKI	GCWGC	1 cut(s)	827
ApoI	RAATTY	2 cut(s)	1517, 1973
ArsI	GACNNNNNNTTYG	6 cut(s)	746, 778, 1441, 1473, 1852, 1884
AsiGI	ACCGGT	4 cut(s)	614, 796, 844, 1739
Asp700I	GAANNNNTTC	2 cut(s)	83, 398
AspLEI	GCGC	2 cut(s)	201, 1048
AspS9I	GGNCC	1 cut(s)	1899
AsuC2I	CCSGG	3 cut(s)	71, 260, 1631
AvaI	CYCGRG	2 cut(s)	334, 530
BarI	GAAGNNNNNNTAC	4 cut(s)	400, 432, 1735, 1767
BbsI	GAAGAC	3 cut(s)	651, 972, 1711
BbvI	GCAGC	1 cut(s)	839
BccI	CCATC	3 cut(s)	109, 1198, 1282
BceAI	ACGGC	5 cut(s)	95, 547, 1033, 1860, 1878
BciT130I	CCWGG	1 cut(s)	678
BclI	TGATCA	2 cut(s)	295, 1917
BcnI	CCSGG	3 cut(s)	71, 260, 1631
BcoDI	GTCTC	6 cut(s)	260, 272, 902, 926, 1354, 1400
BcuI	ACTAGT	1 cut(s)	1262
BfaI	CTAG	3 cut(s)	917, 1263, 1844
BfrI	CTTAAG	2 cut(s)	1307, 1532
BisI	GCNGC	3 cut(s)	202, 828, 1096
BlsI	GCNGC	3 cut(s)	203, 829, 1097
Bme1390I	CCNGG	4 cut(s)	71, 260, 678, 1631
BmeRI	GACNNNNNGTC	1 cut(s)	285
BmeT110I	CYCGRG	2 cut(s)	334, 530
BmgBI	CACGTC	3 cut(s)	762, 979, 1689
BmgT120I	GGNCC	1 cut(s)	1899
BmiI	GGNNCC	1 cut(s)	1773
BmrFI	CCNGG	4 cut(s)	71, 260, 678, 1631
BmsI	GCATC	1 cut(s)	1321
BoxI	GACNNNNGTC	1 cut(s)	189
BpiI	GAAGAC	3 cut(s)	651, 972, 1711
BplI	GAGNNNNNCTC	4 cut(s)	311, 343, 1225, 1257
Bpu10I	CCTNAGC	2 cut(s)	386, 996
BpuEI	CTTGAG	3 cut(s)	201, 338, 1467
BpuMI	CCSGG	3 cut(s)	71, 260, 1631
BsaHI	GRCGYC	2 cut(s)	108, 204
BsaI	GGTCTC	3 cut(s)	260, 902, 926
BsaJI	CCNNGG	4 cut(s)	259, 676, 1522, 1552
BsaWI	WCCGGW	9 cut(s)	274, 404, 476, 614, 796, 844, 957, 1327, 1739
BsaXI	ACNNNNNCTCC	4 cut(s)	74, 104, 458, 488
Bsc4I	CCNNNNNNNGG	3 cut(s)	737, 1076, 1755
Bse118I	RCCGGY	4 cut(s)	614, 796, 844, 1739
Bse1I	ACTGG	3 cut(s)	600, 1138, 1345
Bse3DI	GCAATG	2 cut(s)	1128, 1809
BseBI	CCWGG	1 cut(s)	678
BseDI	CCNNGG	4 cut(s)	259, 676, 1522, 1552
BseGI	GGATG	3 cut(s)	101, 1312, 1545
BseLI	CCNNNNNNNGG	3 cut(s)	737, 1076, 1755
BseMI	GCAATG	2 cut(s)	1128, 1809
BseMII	CTCAG	2 cut(s)	497, 529
BseNI	ACTGG	3 cut(s)	600, 1138, 1345
BseRI	GAGGAG	8 cut(s)	29, 32, 35, 348, 1119, 1122, 1125, 1128
BseXI	GCAGC	1 cut(s)	839
Bsh1236I	CGCG	3 cut(s)	201, 225, 656
BshFI	GGCC	1 cut(s)	1900
BshTI	ACCGGT	4 cut(s)	614, 796, 844, 1739
BsiHKCI	CYCGRG	2 cut(s)	334, 530
BslFI	GGGAC	1 cut(s)	1668
BslI	CCNNNNNNNGG	3 cut(s)	737, 1076, 1755
BsmAI	GTCTC	6 cut(s)	260, 272, 902, 926, 1354, 1400
BsmFI	GGGAC	1 cut(s)	1668
BsnI	GGCC	1 cut(s)	1900
Bso31I	GGTCTC	3 cut(s)	260, 902, 926
BsoBI	CYCGRG	2 cut(s)	334, 530
Bsp1407I	TGTACA	1 cut(s)	1125
Bsp19I	CCATGG	1 cut(s)	1522
BspACI	CCGC	9 cut(s)	60, 138, 201, 560, 687, 711, 1095, 1199, 1749
BspANI	GGCC	1 cut(s)	1900
BspCNI	CTCAG	2 cut(s)	498, 528
BspFNI	CGCG	3 cut(s)	201, 225, 656
BspLI	GGNNCC	1 cut(s)	1773
BspPI	GGATC	3 cut(s)	503, 1021, 1183
BspTI	CTTAAG	2 cut(s)	1307, 1532
BspTNI	GGTCTC	3 cut(s)	260, 902, 926
BsrDI	GCAATG	2 cut(s)	1128, 1809
BsrFI	RCCGGY	4 cut(s)	614, 796, 844, 1739
BsrGI	TGTACA	1 cut(s)	1125
BsrI	ACTGG	3 cut(s)	600, 1138, 1345
BssAI	RCCGGY	4 cut(s)	614, 796, 844, 1739
BssECI	CCNNGG	4 cut(s)	259, 676, 1522, 1552
BssNI	GRCGYC	2 cut(s)	108, 204
BssT1I	CCWWGG	2 cut(s)	1522, 1552
Bst2UI	CCWGG	1 cut(s)	678
Bst4CI	ACNGT	6 cut(s)	191, 248, 821, 869, 1132, 1228
BstACI	GRCGYC	2 cut(s)	108, 204
BstAFI	CTTAAG	2 cut(s)	1307, 1532
BstAUI	TGTACA	1 cut(s)	1125
BstDEI	CTNAG	7 cut(s)	284, 311, 386, 506, 515, 996, 1726
BstDSI	CCRYGG	1 cut(s)	1522
BstF5I	GGATG	3 cut(s)	101, 1312, 1545
BstFNI	CGCG	3 cut(s)	201, 225, 656
BstHHI	GCGC	2 cut(s)	201, 1048
BstMAI	GTCTC	6 cut(s)	260, 272, 902, 926, 1354, 1400
BstMWI	GCNNNNNNNGC	2 cut(s)	210, 997
BstNI	CCWGG	1 cut(s)	678
BstPAI	GACNNNNGTC	1 cut(s)	189
BstSCI	CCNGG	4 cut(s)	69, 258, 676, 1629
BstUI	CGCG	3 cut(s)	201, 225, 656
BstV1I	GCAGC	1 cut(s)	839
BstV2I	GAAGAC	3 cut(s)	651, 972, 1711
BstX2I	RGATCY	1 cut(s)	1026
BstXI	CCANNNNNNTGG	3 cut(s)	1352, 1388, 1446
BstYI	RGATCY	1 cut(s)	1026
BsuRI	GGCC	1 cut(s)	1900
BtgI	CCRYGG	1 cut(s)	1522
BtgZI	GCGATG	3 cut(s)	845, 1713, 1869
BtrI	CACGTC	3 cut(s)	762, 979, 1689
BtsCI	GGATG	3 cut(s)	101, 1312, 1545
BtsI	GCAGTG	1 cut(s)	1041
BtsIMutI	CAGTG	1 cut(s)	1041
CfoI	GCGC	2 cut(s)	201, 1048
Cfr10I	RCCGGY	4 cut(s)	614, 796, 844, 1739
Cfr13I	GGNCC	1 cut(s)	1899
CseI	GACGC	4 cut(s)	116, 193, 214, 645
Csp6I	GTAC	4 cut(s)	408, 1126, 1185, 1378
CspAI	ACCGGT	4 cut(s)	614, 796, 844, 1739
CviAII	CATG	3 cut(s)	1412, 1455, 1523
CviQI	GTAC	4 cut(s)	408, 1126, 1185, 1378
DdeI	CTNAG	7 cut(s)	284, 311, 386, 506, 515, 996, 1726
DraI	TTTAAA	1 cut(s)	525
DriI	GACNNNNNGTC	1 cut(s)	285
Eam1105I	GACNNNNNGTC	1 cut(s)	285
Eco130I	CCWWGG	2 cut(s)	1522, 1552
Eco31I	GGTCTC	3 cut(s)	260, 902, 926
Eco57I	CTGAAG	3 cut(s)	810, 1137, 1980
Eco88I	CYCGRG	2 cut(s)	334, 530
EcoRII	CCWGG	1 cut(s)	676
EcoT14I	CCWWGG	2 cut(s)	1522, 1552
ErhI	CCWWGG	2 cut(s)	1522, 1552
FaeI	CATG	3 cut(s)	1415, 1458, 1526
FaqI	GGGAC	1 cut(s)	1668
FatI	CATG	3 cut(s)	1411, 1454, 1522
FauI	CCCGC	1 cut(s)	567
FbaI	TGATCA	2 cut(s)	295, 1917
FblI	GTMKAC	1 cut(s)	511
Fnu4HI	GCNGC	3 cut(s)	202, 828, 1096
FokI	GGATG	3 cut(s)	88, 1299, 1532
Fsp4HI	GCNGC	3 cut(s)	202, 828, 1096
FspBI	CTAG	3 cut(s)	917, 1263, 1844
GlaI	GCGC	2 cut(s)	200, 1047
GluI	GCNGC	3 cut(s)	202, 828, 1096
HaeIII	GGCC	1 cut(s)	1900
HgaI	GACGC	4 cut(s)	116, 193, 214, 645
HhaI	GCGC	2 cut(s)	201, 1048
Hin1I	GRCGYC	2 cut(s)	108, 204
Hin1II	CATG	3 cut(s)	1415, 1458, 1526
Hin6I	GCGC	2 cut(s)	199, 1046
HinP1I	GCGC	2 cut(s)	199, 1046
HincII	GTYRAC	4 cut(s)	444, 512, 630, 1135
HindII	GTYRAC	4 cut(s)	444, 512, 630, 1135
HindIII	AAGCTT	3 cut(s)	155, 1335, 1569
HpaI	GTTAAC	2 cut(s)	444, 630
Hpy188I	TCNGA	7 cut(s)	106, 507, 582, 790, 1287, 1317, 1982
HpyAV	CCTTC	4 cut(s)	44, 103, 136, 1094
HpyCH4III	ACNGT	6 cut(s)	191, 248, 821, 869, 1132, 1228
HpyCH4IV	ACGT	5 cut(s)	594, 761, 978, 1190, 1688
HpyF10VI	GCNNNNNNNGC	2 cut(s)	210, 997
HpyF3I	CTNAG	7 cut(s)	284, 311, 386, 506, 515, 996, 1726
HpySE526I	ACGT	5 cut(s)	594, 761, 978, 1190, 1688
Hsp92I	GRCGYC	2 cut(s)	108, 204
Hsp92II	CATG	3 cut(s)	1415, 1458, 1526
HspAI	GCGC	2 cut(s)	199, 1046
Ksp22I	TGATCA	2 cut(s)	295, 1917
KspAI	GTTAAC	2 cut(s)	444, 630
LmnI	GCTCC	2 cut(s)	1246, 1771
Lsp1109I	GCAGC	1 cut(s)	839
LweI	GCATC	1 cut(s)	1321
MaeI	CTAG	3 cut(s)	917, 1263, 1844
MaeII	ACGT	5 cut(s)	594, 761, 978, 1190, 1688
MaeIII	GTNAC	6 cut(s)	602, 642, 1222, 1556, 1576, 1879
MflI	RGATCY	1 cut(s)	1026
MluCI	AATT	5 cut(s)	423, 984, 1031, 1517, 1973
MluI	ACGCGT	1 cut(s)	654
MlyI	GAGTC	8 cut(s)	83, 274, 507, 517, 631, 675, 1601, 1849
MmeI	TCCRAC	2 cut(s)	129, 1767
MroXI	GAANNNNTTC	2 cut(s)	83, 398
MspA1I	CMGCKG	1 cut(s)	140
MspCI	CTTAAG	2 cut(s)	1307, 1532
MspR9I	CCNGG	4 cut(s)	71, 260, 678, 1631
MvaI	CCWGG	1 cut(s)	678
MvnI	CGCG	3 cut(s)	201, 225, 656
MwoI	GCNNNNNNNGC	2 cut(s)	210, 997
NciI	CCSGG	3 cut(s)	71, 260, 1631
NcoI	CCATGG	1 cut(s)	1522
NlaIII	CATG	3 cut(s)	1415, 1458, 1526
NlaIV	GGNNCC	1 cut(s)	1773
NmuCI	GTSAC	3 cut(s)	1222, 1556, 1576
PaeR7I	CTCGAG	1 cut(s)	530
PcsI	WCGNNNNNNNCGW	3 cut(s)	229, 1253, 1760
PdmI	GAANNNNTTC	2 cut(s)	83, 398
PfeI	GAWTC	7 cut(s)	343, 484, 648, 1065, 1282, 1318, 1927
PflFI	GACNNNGTC	1 cut(s)	110
PinAI	ACCGGT	4 cut(s)	614, 796, 844, 1739
PkrI	GCNGC	3 cut(s)	203, 829, 1097
PleI	GAGTC	8 cut(s)	83, 274, 507, 516, 631, 675, 1601, 1849
PpsI	GAGTC	8 cut(s)	83, 274, 507, 516, 631, 675, 1601, 1849
PshAI	GACNNNNGTC	1 cut(s)	189
PsiI	TTATAA	1 cut(s)	950
Psp1406I	AACGTT	1 cut(s)	594
Psp6I	CCWGG	1 cut(s)	676
PspGI	CCWGG	1 cut(s)	676
PspN4I	GGNNCC	1 cut(s)	1773
PspPI	GGNCC	1 cut(s)	1899
PsuI	RGATCY	1 cut(s)	1026
PsyI	GACNNNGTC	1 cut(s)	110
RsaI	GTAC	4 cut(s)	409, 1127, 1186, 1379
RsaNI	GTAC	4 cut(s)	408, 1126, 1185, 1378
SalI	GTCGAC	1 cut(s)	510
SatI	GCNGC	3 cut(s)	202, 828, 1096
Sau96I	GGNCC	1 cut(s)	1899
SchI	GAGTC	8 cut(s)	83, 274, 507, 517, 631, 675, 1601, 1849
ScrFI	CCNGG	4 cut(s)	71, 260, 678, 1631
SfaNI	GCATC	1 cut(s)	1321
Sfr274I	CTCGAG	1 cut(s)	530
SgrAI	CRCCGGYG	1 cut(s)	844
SlaI	CTCGAG	1 cut(s)	530
SmlI	CTYRAG	6 cut(s)	216, 317, 530, 1307, 1482, 1532
SmoI	CTYRAG	6 cut(s)	216, 317, 530, 1307, 1482, 1532
SpeI	ACTAGT	1 cut(s)	1262
Sse9I	AATT	5 cut(s)	423, 984, 1031, 1517, 1973
SsiI	CCGC	9 cut(s)	60, 138, 201, 560, 687, 711, 1095, 1199, 1749
SspMI	CTAG	3 cut(s)	917, 1263, 1844
StyD4I	CCNGG	4 cut(s)	69, 258, 676, 1629
StyI	CCWWGG	2 cut(s)	1522, 1552
TaaI	ACNGT	6 cut(s)	191, 248, 821, 869, 1132, 1228
TaiI	ACGT	5 cut(s)	597, 764, 981, 1193, 1691
TasI	AATT	5 cut(s)	423, 984, 1031, 1517, 1973
TatI	WGTACW	1 cut(s)	1125
TauI	GCSGC	2 cut(s)	204, 1098
TfiI	GAWTC	7 cut(s)	343, 484, 648, 1065, 1282, 1318, 1927
TscAI	CASTG	1 cut(s)	1048
TseFI	GTSAC	3 cut(s)	1222, 1556, 1576
TseI	GCWGC	1 cut(s)	827
Tsp45I	GTSAC	3 cut(s)	1222, 1556, 1576
TspDTI	ATGAA	3 cut(s)	576, 1400, 1922
TspGWI	ACGGA	7 cut(s)	38, 477, 564, 615, 720, 1092, 1233
TspRI	CASTG	1 cut(s)	1048
Tth111I	GACNNNGTC	1 cut(s)	110
Vha464I	CTTAAG	2 cut(s)	1307, 1532
XapI	RAATTY	2 cut(s)	1517, 1973
XhoI	CTCGAG	1 cut(s)	530
XmiI	GTMKAC	1 cut(s)	511
XmnI	GAANNNNTTC	2 cut(s)	83, 398
XspI	CTAG	3 cut(s)	917, 1263, 1844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.