pycom01g08530
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009275
Physical Location & Seq
Reverse (-)
9477483 .. 9478475
993 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g08530.1

Sequence Viewer

Length: 864 bp
ATGAACCAGTCCTTCCTTTTCTTCAACGTCTCCTTTAACAACCTGACTGGCCCGGTTCCCGGTTCGACGCGTCGTCGTTTCAGCTCAACCCGGGCCCCATTCTTCGAATCTCTCATTGTCAGTTCCACCTCTCCGGCGTCGGAGCCGCTGGGGGAGAGCACTGCGCAGTCTCAGGGAGTTATTTTGTCTCCGCCTTCCCCGAAAAACCACAAGAAAACCGGCGTCATCCTCGGCATCGCAATCGGGGTCTTGCTCCTCATCGCGGCCGTCTTATGCCTCTTTGCGGTGGCTCGAAACCATAACAAAACCATCACTTACACAGACACAAAACCGTCGCCAATCACCTCACCAGCCAATCGCATTCACTCTAATCCAAAAAATTTGAGAACCATCGAAGTAAAGACGGTTGAACAAGCAGCTCCTCCGAGAGCAATTCCGAGGAGCGGGAACTTGATTTTTTACTACAGGGAGGCTCAGCTATGCGGTCTGGAGCAGCTGATGAGTGCCTCGGCTGAGTTGCTCGGGAGGGGTAGCATTGGGACTACGCAAAGGCAGGAAGAAGGGGAGAGATGCGGAGACTTGGAGGAAAAACACAGGCTCTATGGTGAGCTTGAAGCAATCTATAACCTTGCAAAGATGGGGGAGGGTAATCAGAATCAAACTGGCTCTTGCTCTGCTCTCACCGACGAAAACTGCCTGAAAAATGTGGGATTTCCTGTGGTTTTTGGTGATTTTAATGGCCTCAATGCTGCTCCAGCTGATACATTTAGGGTGGATAACAGATCGGAAGACTCCATTGGAGAAGAGGTGTTGATAAGGAAGATTCAAAAGAACATGAGGATGAGGAAAATGAAGGTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

288

Amino Acids

31.4

Weight (kDa)

9.11

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 165
AccBSI CCGCTC 1 cut(s) 444
AccII CGCG 2 cut(s) 70, 263
AciI CCGC 7 cut(s) 146, 191, 263, 284, 444, 483, 573
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 1 cut(s) 379
AcyI GRCGYC 2 cut(s) 137, 222
AfiI CCNNNNNNNGG 4 cut(s) 59, 262, 283, 443
AflIII ACRYGT 1 cut(s) 68
AgsI TTSAA 4 cut(s) 25, 410, 614, 827
AhdI GACNNNNNGTC 1 cut(s) 72
AjuI GAANNNNNNNTTGG 2 cut(s) 780, 812
AluBI AGCT 6 cut(s) 84, 419, 478, 496, 610, 758
AluI AGCT 6 cut(s) 84, 419, 478, 496, 610, 758
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 4 cut(s) 34, 174, 192, 570
Ama87I CYCGRG 2 cut(s) 90, 521
AoxI GGCC 4 cut(s) 49, 93, 264, 739
ApaI GGGCCC 1 cut(s) 97
ApeKI GCWGC 3 cut(s) 416, 493, 749
ApoI RAATTY 1 cut(s) 379
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 3 cut(s) 50, 93, 94
AsuC2I CCSGG 4 cut(s) 53, 60, 91, 92
AsuHPI GGTGA 5 cut(s) 334, 339, 617, 673, 740
AsuII TTCGAA 1 cut(s) 105
AvaI CYCGRG 2 cut(s) 90, 521
BaeGI GKGCMC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 161
BbvI GCAGC 3 cut(s) 428, 505, 736
BccI CCATC 3 cut(s) 317, 398, 631
BceAI ACGGC 1 cut(s) 251
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BcnI CCSGG 4 cut(s) 53, 60, 91, 92
BcoDI GTCTC 4 cut(s) 34, 174, 192, 570
BfmI CTRYAG 1 cut(s) 463
BisI GCNGC 5 cut(s) 146, 264, 417, 494, 750
BlpI GCTNAGC 1 cut(s) 474
BlsI GCNGC 5 cut(s) 147, 265, 418, 495, 751
Bme1390I CCNGG 4 cut(s) 53, 60, 91, 92
BmeRI GACNNNNNGTC 1 cut(s) 72
BmeT110I CYCGRG 2 cut(s) 90, 521
BmgT120I GGNCC 3 cut(s) 50, 93, 94
BmiI GGNNCC 4 cut(s) 57, 95, 96, 144
BmrFI CCNGG 4 cut(s) 53, 60, 91, 92
BmsI GCATC 2 cut(s) 243, 560
BpiI GAAGAC 1 cut(s) 795
BpmI CTGGAG 2 cut(s) 509, 738
Bpu1102I GCTNAGC 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 105
BpuMI CCSGG 4 cut(s) 53, 60, 91, 92
BsaHI GRCGYC 2 cut(s) 137, 222
BsaJI CCNNGG 4 cut(s) 90, 229, 437, 507
BsaXI ACNNNNNCTCC 2 cut(s) 792, 822
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 262, 283, 443
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 7, 52, 667
BseDI CCNNGG 4 cut(s) 90, 229, 437, 507
BseGI GGATG 2 cut(s) 225, 846
BseLI CCNNNNNNNGG 4 cut(s) 59, 262, 283, 443
BseMII CTCAG 3 cut(s) 185, 488, 504
BseNI ACTGG 3 cut(s) 7, 52, 667
BseRI GAGGAG 3 cut(s) 245, 411, 454
BseSI GKGCMC 1 cut(s) 97
BseX3I CGGCCG 1 cut(s) 264
BseXI GCAGC 3 cut(s) 428, 505, 736
BseYI CCCAGC 1 cut(s) 148
Bsh1236I CGCG 2 cut(s) 70, 263
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 4 cut(s) 51, 95, 266, 741
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 161
BsiHKCI CYCGRG 2 cut(s) 90, 521
BsiSI CCGG 5 cut(s) 53, 60, 91, 134, 219
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 4 cut(s) 59, 262, 283, 443
BsmAI GTCTC 4 cut(s) 34, 174, 192, 570
BsmBI CGTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 553
BsmI GAATGC 1 cut(s) 360
BsnI GGCC 4 cut(s) 51, 95, 266, 741
BsoBI CYCGRG 2 cut(s) 90, 521
Bsp119I TTCGAA 1 cut(s) 105
Bsp120I GGGCCC 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 97, 161
Bsp143I GATC 1 cut(s) 782
Bsp1720I GCTNAGC 1 cut(s) 474
BspACI CCGC 7 cut(s) 146, 191, 263, 284, 444, 483, 573
BspANI GGCC 4 cut(s) 51, 95, 266, 741
BspCNI CTCAG 3 cut(s) 184, 487, 505
BspFNI CGCG 2 cut(s) 70, 263
BspLI GGNNCC 4 cut(s) 57, 95, 96, 144
BspT104I TTCGAA 1 cut(s) 105
BsrBI CCGCTC 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 3 cut(s) 7, 52, 667
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 4 cut(s) 90, 229, 437, 507
BssMI GATC 1 cut(s) 782
BssNI GRCGYC 2 cut(s) 137, 222
Bst4CI ACNGT 2 cut(s) 333, 406
Bst6I CTCTTC 1 cut(s) 798
BstACI GRCGYC 2 cut(s) 137, 222
BstBI TTCGAA 1 cut(s) 105
BstDEI CTNAG 3 cut(s) 171, 474, 513
BstF5I GGATG 2 cut(s) 225, 846
BstFNI CGCG 2 cut(s) 70, 263
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 1 cut(s) 785
BstMAI GTCTC 4 cut(s) 34, 174, 192, 570
BstMBI GATC 1 cut(s) 782
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 1 cut(s) 755
BstSCI CCNGG 4 cut(s) 51, 58, 89, 90
BstSFI CTRYAG 1 cut(s) 463
BstSLI GKGCMC 1 cut(s) 97
BstUI CGCG 2 cut(s) 70, 263
BstV1I GCAGC 3 cut(s) 428, 505, 736
BstV2I GAAGAC 1 cut(s) 795
BstZI CGGCCG 1 cut(s) 264
BsuRI GGCC 4 cut(s) 51, 95, 266, 741
BtgZI GCGATG 2 cut(s) 220, 244
BtsCI GGATG 2 cut(s) 225, 846
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 159
CfoI GCGC 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 50, 93, 94
Cfr9I CCCGGG 1 cut(s) 90
CseI GACGC 4 cut(s) 59, 76, 126, 211
CviAII CATG 1 cut(s) 835
DdeI CTNAG 3 cut(s) 171, 474, 513
DpnI GATC 1 cut(s) 784
DpnII GATC 1 cut(s) 782
DriI GACNNNNNGTC 1 cut(s) 72
EaeI YGGCCR 1 cut(s) 264
EagI CGGCCG 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 798
Eam1105I GACNNNNNGTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 798
EciI GGCGGA 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 97
Eco52I CGGCCG 1 cut(s) 264
Eco88I CYCGRG 2 cut(s) 90, 521
EcoO109I RGGNCCY 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 97
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 1 cut(s) 838
FaiI YATR 6 cut(s) 274, 300, 481, 603, 624, 836
FaqI GGGAC 1 cut(s) 553
FatI CATG 1 cut(s) 834
FauI CCCGC 1 cut(s) 437
Fnu4HI GCNGC 5 cut(s) 146, 264, 417, 494, 750
FokI GGATG 2 cut(s) 212, 853
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 5 cut(s) 146, 264, 417, 494, 750
FspI TGCGCA 1 cut(s) 165
GlaI GCGC 1 cut(s) 165
GluI GCNGC 5 cut(s) 146, 264, 417, 494, 750
GsaI CCCAGC 1 cut(s) 152
GsuI CTGGAG 2 cut(s) 509, 738
HaeIII GGCC 4 cut(s) 51, 95, 266, 741
HapII CCGG 5 cut(s) 53, 60, 91, 134, 219
HgaI GACGC 4 cut(s) 59, 76, 126, 211
HhaI GCGC 1 cut(s) 166
Hin1I GRCGYC 2 cut(s) 137, 222
Hin1II CATG 1 cut(s) 838
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HinfI GANTC 4 cut(s) 107, 655, 791, 823
HpaII CCGG 5 cut(s) 53, 60, 91, 134, 219
HphI GGTGA 5 cut(s) 334, 339, 617, 673, 740
Hpy188I TCNGA 5 cut(s) 142, 426, 438, 654, 787
Hpy188III TCNNGA 2 cut(s) 488, 523
Hpy99I CGWCG 6 cut(s) 70, 75, 78, 142, 337, 689
HpyAV CCTTC 4 cut(s) 22, 204, 554, 847
HpyCH4III ACNGT 2 cut(s) 333, 406
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 632
HpyF10VI GCNNNNNNNGC 1 cut(s) 755
HpyF3I CTNAG 3 cut(s) 171, 474, 513
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 2 cut(s) 137, 222
Hsp92II CATG 1 cut(s) 838
HspAI GCGC 1 cut(s) 164
Kzo9I GATC 1 cut(s) 782
LmnI GCTCC 6 cut(s) 142, 258, 424, 441, 490, 757
Lsp1109I GCAGC 3 cut(s) 428, 505, 736
LweI GCATC 2 cut(s) 243, 560
MaeII ACGT 1 cut(s) 27
MalI GATC 1 cut(s) 784
MbiI CCGCTC 1 cut(s) 444
MboI GATC 1 cut(s) 782
MboII GAAGA 6 cut(s) 13, 94, 569, 800, 815, 832
MhlI GDGCHC 2 cut(s) 97, 161
MluCI AATT 3 cut(s) 379, 432, 859
MluI ACGCGT 1 cut(s) 68
MlyI GAGTC 1 cut(s) 785
MmeI TCCRAC 1 cut(s) 120
MseI TTAA 3 cut(s) 36, 735, 862
MslI CAYNNNNRTG 1 cut(s) 839
MspA1I CMGCKG 3 cut(s) 148, 496, 758
MspI CCGG 5 cut(s) 53, 60, 91, 134, 219
MspR9I CCNGG 4 cut(s) 53, 60, 91, 92
Mva1269I GAATGC 1 cut(s) 360
MvnI CGCG 2 cut(s) 70, 263
MwoI GCNNNNNNNGC 1 cut(s) 755
NciI CCSGG 4 cut(s) 53, 60, 91, 92
NdeII GATC 1 cut(s) 782
NlaIII CATG 1 cut(s) 838
NlaIV GGNNCC 4 cut(s) 57, 95, 96, 144
NmeAIII GCCGAG 2 cut(s) 210, 488
NsbI TGCGCA 1 cut(s) 165
NspV TTCGAA 1 cut(s) 105
PctI GAATGC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 107, 655, 823
PkrI GCNGC 5 cut(s) 147, 265, 418, 495, 751
PleI GAGTC 1 cut(s) 785
PpsI GAGTC 1 cut(s) 785
PspFI CCCAGC 1 cut(s) 148
PspN4I GGNNCC 4 cut(s) 57, 95, 96, 144
PspOMI GGGCCC 1 cut(s) 93
PspPI GGNCC 3 cut(s) 50, 93, 94
PvuII CAGCTG 2 cut(s) 496, 758
RseI CAYNNNNRTG 1 cut(s) 839
SaqAI TTAA 3 cut(s) 36, 735, 862
SatI GCNGC 5 cut(s) 146, 264, 417, 494, 750
Sau3AI GATC 1 cut(s) 782
Sau96I GGNCC 3 cut(s) 50, 93, 94
SchI GAGTC 1 cut(s) 785
ScrFI CCNGG 4 cut(s) 53, 60, 91, 92
SduI GDGCHC 2 cut(s) 97, 161
SfaNI GCATC 2 cut(s) 243, 560
SfcI CTRYAG 1 cut(s) 463
SfuI TTCGAA 1 cut(s) 105
SmaI CCCGGG 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 839
Sse9I AATT 3 cut(s) 379, 432, 859
SsiI CCGC 7 cut(s) 146, 191, 263, 284, 444, 483, 573
StyD4I CCNGG 4 cut(s) 51, 58, 89, 90
TaaI ACNGT 2 cut(s) 333, 406
TaiI ACGT 1 cut(s) 30
TaqI TCGA 4 cut(s) 65, 105, 292, 393
TasI AATT 3 cut(s) 379, 432, 859
TauI GCSGC 2 cut(s) 148, 266
TfiI GAWTC 3 cut(s) 107, 655, 823
Tru1I TTAA 3 cut(s) 36, 735, 862
Tru9I TTAA 3 cut(s) 36, 735, 862
TscAI CASTG 1 cut(s) 166
TseI GCWGC 3 cut(s) 416, 493, 749
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 90
TspRI CASTG 1 cut(s) 166
XapI RAATTY 1 cut(s) 379
XmaI CCCGGG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.