Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009275

Physical Location & Seq

Genomic Coordinates Reverse (-)

15604750 .. 15608178

Gene Structure 3429 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1176600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGATTCCCTTCCTCCTCCTCCTCCTCCTCATCGCCACCGCCGTCCACCCCTCCACTTCCCTTCCATTCCCACCGCCGCCTCCAAACCTCCTCCTCCCATCCTCCGACGCCGTCGCCCTCCTGTCCTTCAAATCCCAAGCCGACCTCAACAACAAGCTCCTCTACACCCTCAACGAGCGCTTCGACTACTGCCAATGGCAGGGCGTCAAGTGCTCCCAGGGCCGCGTCGTCCGCTTCGTCCTCCAGTCCTTCTCTCTCCGTGGCTCCTTCCCTCCCGACACCCTCTCCCGCCTCGACCAGCTCCGCGTCCTCAGCCTCCACAACAACTCCCTCTCCGGCCCCATCCCCGACCTCTCCCCTCTCCAGAACCTCAAGTCCCTCTTCCTCAACCGCAACTCCTTCTCCGGCTTCTTTCCCCCCTCCATCCTCGCCCTCCACCGCCTCACCGTCCTCGATCTCTCCTTCAACGACCTCTCCGGTCCCATTCCCGACAACCTCTCTGGCCTGGACCGGCTCACCTCACTCCAGCTCCAGTCCAACCGCTTCAACGGCTCCCTCCCGGGTCTGAACCAGTCCTTCCTTTTAATCTTCAACGTCTCCTTTAACAACCTGACCGGCCCGGTTCCCCCTTCCCTGTCCCGGTTCGACGCGTCGTCGTTTCAGCTCAACCCGGGGCTCTGCGGCGAGACTGTGAACCGGGCGTGCAGCTCGCACGCCCCATTCTTCGAATCTCGCAATGCCAGTTCAACCTCTCCGGCGTCGGAGCCGCTGGGGGAGAGCACTGCACAGTCTCAGGGAGTTGTTCTGTCTCCGCCTTCCCCGAAAAACCACAAGAAAACTGGCGTCATCCTCGGCGTCGCAATCGGGGTTTCGCTCCTCGTCGCCGCCGTCTTATGCCTCTTTGCGGTGGCTCGAAACCATAACAAAACCATCACTTACACAGACACAAAACCGTCGCCAATTACCTCACCAGCCAATCGCATTCACTCTAATCCAAACAATTTCAGAACCATCGAAGCACAGATCCCAGAACGGAGGGAGGTGGTGCAGTTTTCCGACAAGGTAAAGACAGTTGAACACGCAGCTCCTCCGAGAGCAATTCCAAGGAGCGGGAACTTGATTTTTTGCTACGGGGAGGCTCAGCTATACAGTCTGGAGCAGCTGATGAGAGCCTCGGCGGAGTTGCTCGGGAGGGGTAGCATTGGGACTACGTACAAGGCGGTGCTGGACAACCAGCTGATTGTGACGGTGAAGAGATTGGACGCCGGCAAGACGGCGATTACAAGCGGAGAAGCGTTTGAGGAGCATATGGACGTTGTGGGTGGGTTGCGGCATCCGTATCTGGTTCCGGTGAGGGCTTATTTTCAGGCAAAGGGAGAGCGGCTGGTGATCTATGATTACCAACCCAACGGCAGTCTATTCAATCTCATTCACGGTTCGAAATCAACTAGAGCAAGACCTCTGCATTGGACATCATGCTTGAAGATAGCTGAAGATGTAGCTCAAGGCCTTGCTTACATTCACCAATCGTCGTCCCTCATCCATGGCAATCTCAAGTCCTCCAATGTTCTCCTCGGAGGCGACTTCGAGGCCTGTCTCACGGACTACGGCCTTGCCTTCTTTGCGGACACTTCTGCAAATGAAGATCCTGATTCTGCAGGCTATAAAGCCCCCGAGATTCGCAAGTCCAGTAGGCGAGCCACGTCCAAATCCGATGTCTATGCCTTCGGCATCTTATTGCTTGAGCTCTTGACGGGTAAACATCCGTCCCAACATCCGCTTCTCGTGCCCACGGACGTGCCTGATTGGGTCAGAGTAATGAGGGATGACGATGTTGGGGATGACAATCAACTGGGAATGCTCACCGAGGTCGCTTGTATTTGTAGTTTGACGTCTCCGGAACAGAGGCCGGCAATGTGGCAAGTGTTGAAGATGATACAGGAGATTAAAGAGAGTGTGATGACTGACGATAACGCAGACGTCGGATTTTCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

666

Amino Acids

72.67

Weight (kDa)

7.25

Isoelectric Point (pI)

45.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	123 - 182	1.9e-07	Leucine rich repeat
Pkinase	PF00069	394 - 591	1.3e-30	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	394 - 644	3.9e-29	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50230 AT3G50230 AT5G67200
fragaria_vesca	FvH4_1g16410
malus_domestica	MD02G1176600.v1.1 MD08G1095300.v1.1
prunus_persica	Prupe.6G231100_v2.0.a1
pyrus_communis	pycom01g08530 pycom02g14290 pycom15g07270
rosa_chinensis	RchiOBHm_Chr2g0106291
rosa_laevigata	RLG00000017486
rosa_multiflora	Rmu_sc0020846.1_g000001 Rmu_sc0021309.1_g000001 Rmu_ssc0000338.1_g000005
rosa_roxburghii	Rroxscaffold_2G00137300
rosa_rugosa	Rorug02G0134500
rosa_samantha	Rh2AG185300 Rh2BG195600 Rh2CG190400 Rh2DG191500
rosa_wichuraiana	Rw2G014470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	1895, 1983
AccBSI	CCGCTC	2 cut(s)	1110, 1381
AccII	CGCG	3 cut(s)	225, 306, 650
AccIII	TCCGGA	1 cut(s)	1897
AclWI	GGATC	2 cut(s)	1018, 1640
AcuI	CTGAAG	1 cut(s)	1512
AcyI	GRCGYC	8 cut(s)	108, 204, 758, 843, 855, 1263, 1892, 1980
AfaI	GTAC	1 cut(s)	1214
AfeI	AGCGCT	1 cut(s)	179
AfiI	CCNNNNNNNGG	4 cut(s)	199, 639, 904, 1109
AflIII	ACRYGT	1 cut(s)	648
AgsI	TTSAA	9 cut(s)	130, 466, 547, 592, 747, 1076, 1423, 1483, 1930
AhdI	GACNNNNNGTC	1 cut(s)	652
AjiI	CACGTC	2 cut(s)	1704, 1798
AjnI	CCWGG	2 cut(s)	216, 504
AjuI	GAANNNNNNNTTGG	2 cut(s)	1764, 1796
AleI	CACNNNNGTG	1 cut(s)	1796
AloI	GAACNNNNNNTCC	2 cut(s)	359, 391
Alw21I	GWGCWC	3 cut(s)	215, 782, 1749
Alw26I	GTCTC	6 cut(s)	601, 680, 795, 813, 1601, 1899
AlwI	GGATC	2 cut(s)	1018, 1640
Ama87I	CYCGRG	4 cut(s)	559, 670, 1187, 1673
Aor13HI	TCCGGA	1 cut(s)	1897
Aor51HI	AGCGCT	1 cut(s)	179
AoxI	GGCC	8 cut(s)	220, 337, 502, 616, 1507, 1590, 1609, 1907
ApeKI	GCWGC	3 cut(s)	705, 1082, 1159
ArsI	GACNNNNNNTTYG	2 cut(s)	1701, 1733
AspLEI	GCGC	1 cut(s)	180
AspS9I	GGNCC	5 cut(s)	220, 338, 479, 508, 617
AsuC2I	CCSGG	7 cut(s)	560, 561, 620, 640, 671, 672, 698
AsuHPI	GGTGA	8 cut(s)	436, 508, 960, 1261, 1363, 1399, 1514, 1855
AsuII	TTCGAA	2 cut(s)	726, 1439
AvaI	CYCGRG	4 cut(s)	559, 670, 1187, 1673
AvaII	GGWCC	2 cut(s)	479, 508
BaeGI	GKGCMC	1 cut(s)	1791
BanII	GRGCYC	2 cut(s)	678, 1749
BauI	CACGAG	1 cut(s)	1784
Bbv12I	GWGCWC	3 cut(s)	215, 782, 1749
BbvCI	CCTCAGC	1 cut(s)	311
BbvI	GCAGC	3 cut(s)	717, 1094, 1171
BccI	CCATC	5 cut(s)	106, 350, 431, 938, 1019
BceAI	ACGGC	7 cut(s)	26, 95, 565, 872, 1290, 1426, 1624
BcgI	CGANNNNNNTGC	2 cut(s)	1703, 1737
BciT130I	CCWGG	2 cut(s)	218, 506
BcnI	CCSGG	7 cut(s)	560, 561, 620, 640, 671, 672, 698
BcoDI	GTCTC	6 cut(s)	601, 680, 795, 813, 1601, 1899
BfaI	CTAG	1 cut(s)	1449
BfmI	CTRYAG	1 cut(s)	1656
BfoI	RGCGCY	1 cut(s)	181
BlpI	GCTNAGC	1 cut(s)	1140
Bme1390I	CCNGG	9 cut(s)	218, 506, 560, 561, 620, 640, 671, 672, 698
Bme18I	GGWCC	2 cut(s)	479, 508
BmeRI	GACNNNNNGTC	1 cut(s)	652
BmeT110I	CYCGRG	4 cut(s)	559, 670, 1187, 1673
BmgBI	CACGTC	2 cut(s)	1704, 1798
BmgT120I	GGNCC	5 cut(s)	220, 338, 479, 508, 617
BmiI	GGNNCC	7 cut(s)	265, 340, 481, 553, 624, 765, 1347
BmrFI	CCNGG	9 cut(s)	218, 506, 560, 561, 620, 640, 671, 672, 698
BmrI	ACTGGG	1 cut(s)	1862
BmsI	GCATC	2 cut(s)	1342, 1740
BmuI	ACTGGG	1 cut(s)	1862
BpmI	CTGGAG	5 cut(s)	227, 347, 509, 515, 1175
Bpu10I	CCTNAGC	1 cut(s)	311
Bpu1102I	GCTNAGC	1 cut(s)	1140
Bpu14I	TTCGAA	2 cut(s)	726, 1439
BpuEI	CTTGAG	4 cut(s)	356, 1488, 1538, 1763
BpuMI	CCSGG	7 cut(s)	560, 561, 620, 640, 671, 672, 698
BsaAI	YACGTR	1 cut(s)	1212
BsaBI	GATNNNNATC	1 cut(s)	1845
BsaHI	GRCGYC	8 cut(s)	108, 204, 758, 843, 855, 1263, 1892, 1980
BsaWI	WCCGGW	3 cut(s)	476, 1348, 1897
Bsc4I	CCNNNNNNNGG	4 cut(s)	199, 639, 904, 1109
Bse118I	RCCGGY	4 cut(s)	510, 614, 1265, 1909
Bse1I	ACTGG	7 cut(s)	244, 532, 571, 741, 844, 1689, 1857
Bse3DI	GCAATG	2 cut(s)	742, 1920
Bse8I	GATNNNNATC	1 cut(s)	1845
BseAI	TCCGGA	1 cut(s)	1897
BseBI	CCWGG	2 cut(s)	218, 506
BseJI	GATNNNNATC	1 cut(s)	1845
BseLI	CCNNNNNNNGG	4 cut(s)	199, 639, 904, 1109
BseMI	GCAATG	2 cut(s)	742, 1920
BseMII	CTCAG	3 cut(s)	325, 806, 1154
BseNI	ACTGG	7 cut(s)	244, 532, 571, 741, 844, 1689, 1857
BseSI	GKGCMC	1 cut(s)	1791
BseXI	GCAGC	3 cut(s)	717, 1094, 1171
BseYI	CCCAGC	1 cut(s)	769
BsgI	GTGCAG	3 cut(s)	724, 768, 1067
Bsh1236I	CGCG	3 cut(s)	225, 306, 650
BshFI	GGCC	8 cut(s)	222, 339, 504, 618, 1509, 1592, 1611, 1909
BsiHKAI	GWGCWC	3 cut(s)	215, 782, 1749
BsiHKCI	CYCGRG	4 cut(s)	559, 670, 1187, 1673
BslFI	GGGAC	6 cut(s)	361, 465, 622, 1219, 1519, 1753
BslI	CCNNNNNNNGG	4 cut(s)	199, 639, 904, 1109
BsmAI	GTCTC	6 cut(s)	601, 680, 795, 813, 1601, 1899
BsmBI	CGTCTC	2 cut(s)	601, 1899
BsmFI	GGGAC	6 cut(s)	361, 465, 622, 1219, 1519, 1753
BsmI	GAATGC	2 cut(s)	981, 1863
BsnI	GGCC	8 cut(s)	222, 339, 504, 618, 1509, 1592, 1611, 1909
BsoBI	CYCGRG	4 cut(s)	559, 670, 1187, 1673
Bsp119I	TTCGAA	2 cut(s)	726, 1439
Bsp1286I	GDGCHC	5 cut(s)	215, 678, 782, 1749, 1791
Bsp13I	TCCGGA	1 cut(s)	1897
Bsp143I	GATC	4 cut(s)	454, 1023, 1389, 1645
Bsp1720I	GCTNAGC	1 cut(s)	1140
Bsp19I	CCATGG	1 cut(s)	1543
BspANI	GGCC	8 cut(s)	222, 339, 504, 618, 1509, 1592, 1611, 1909
BspCNI	CTCAG	3 cut(s)	324, 805, 1153
BspEI	TCCGGA	1 cut(s)	1897
BspFNI	CGCG	3 cut(s)	225, 306, 650
BspLI	GGNNCC	7 cut(s)	265, 340, 481, 553, 624, 765, 1347
BspMAI	CTGCAG	1 cut(s)	1660
BspPI	GGATC	2 cut(s)	1018, 1640
BspT104I	TTCGAA	2 cut(s)	726, 1439
BsrBI	CCGCTC	2 cut(s)	1110, 1381
BsrDI	GCAATG	2 cut(s)	742, 1920
BsrFI	RCCGGY	4 cut(s)	510, 614, 1265, 1909
BsrI	ACTGG	7 cut(s)	244, 532, 571, 741, 844, 1689, 1857
BssAI	RCCGGY	4 cut(s)	510, 614, 1265, 1909
BssMI	GATC	4 cut(s)	454, 1023, 1389, 1645
BssNI	GRCGYC	8 cut(s)	108, 204, 758, 843, 855, 1263, 1892, 1980
BssSI	CACGAG	1 cut(s)	1784
BssT1I	CCWWGG	2 cut(s)	1103, 1543
Bst2BI	CACGAG	1 cut(s)	1784
Bst2UI	CCWGG	2 cut(s)	218, 506
Bst4CI	ACNGT	8 cut(s)	448, 691, 789, 954, 1072, 1151, 1249, 1436
Bst6I	CTCTTC	2 cut(s)	386, 1247
BstACI	GRCGYC	8 cut(s)	108, 204, 758, 843, 855, 1263, 1892, 1980
BstBAI	YACGTR	1 cut(s)	1212
BstBI	TTCGAA	2 cut(s)	726, 1439
BstC8I	GCNNGC	7 cut(s)	703, 710, 714, 1267, 1660, 1698, 1911
BstDEI	CTNAG	3 cut(s)	311, 792, 1140
BstDSI	CCRYGG	3 cut(s)	259, 1543, 1791
BstFNI	CGCG	3 cut(s)	225, 306, 650
BstH2I	RGCGCY	1 cut(s)	181
BstHHI	GCGC	1 cut(s)	180
BstKTI	GATC	4 cut(s)	457, 1026, 1392, 1648
BstMAI	GTCTC	6 cut(s)	601, 680, 795, 813, 1601, 1899
BstMBI	GATC	4 cut(s)	454, 1023, 1389, 1645
BstMWI	GCNNNNNNNGC	7 cut(s)	210, 219, 231, 312, 549, 1622, 1786
BstNI	CCWGG	2 cut(s)	218, 506
BstSCI	CCNGG	9 cut(s)	216, 504, 558, 559, 618, 638, 669, 670, 696
BstSFI	CTRYAG	1 cut(s)	1656
BstSLI	GKGCMC	1 cut(s)	1791
BstSNI	TACGTA	1 cut(s)	1212
BstUI	CGCG	3 cut(s)	225, 306, 650
BstV1I	GCAGC	3 cut(s)	717, 1094, 1171
BstX2I	RGATCY	2 cut(s)	1023, 1645
BstYI	RGATCY	2 cut(s)	1023, 1645
BsuRI	GGCC	8 cut(s)	222, 339, 504, 618, 1509, 1592, 1611, 1909
BtgI	CCRYGG	3 cut(s)	259, 1543, 1791
BtgZI	GCGATG	1 cut(s)	16
BtrI	CACGTC	2 cut(s)	1704, 1798
BtsI	GCAGTG	1 cut(s)	780
BtsIMutI	CAGTG	1 cut(s)	780
Cac8I	GCNNGC	7 cut(s)	703, 710, 714, 1267, 1660, 1698, 1911
CfoI	GCGC	1 cut(s)	180
Cfr10I	RCCGGY	4 cut(s)	510, 614, 1265, 1909
Cfr13I	GGNCC	5 cut(s)	220, 338, 479, 508, 617
Cfr9I	CCCGGG	2 cut(s)	559, 670
Csp6I	GTAC	1 cut(s)	1213
CviAII	CATG	2 cut(s)	1476, 1544
CviQI	GTAC	1 cut(s)	1213
DdeI	CTNAG	3 cut(s)	311, 792, 1140
DpnI	GATC	4 cut(s)	456, 1025, 1391, 1647
DpnII	GATC	4 cut(s)	454, 1023, 1389, 1645
DriI	GACNNNNNGTC	1 cut(s)	652
Eam1104I	CTCTTC	2 cut(s)	386, 1247
Eam1105I	GACNNNNNGTC	1 cut(s)	652
EarI	CTCTTC	2 cut(s)	386, 1247
EciI	GGCGGA	2 cut(s)	801, 1193
Ecl136II	GAGCTC	1 cut(s)	1747
Eco105I	TACGTA	1 cut(s)	1212
Eco130I	CCWWGG	2 cut(s)	1103, 1543
Eco147I	AGGCCT	2 cut(s)	1509, 1592
Eco24I	GRGCYC	2 cut(s)	678, 1749
Eco47I	GGWCC	2 cut(s)	479, 508
Eco47III	AGCGCT	1 cut(s)	179
Eco53kI	GAGCTC	1 cut(s)	1747
Eco57I	CTGAAG	1 cut(s)	1512
Eco88I	CYCGRG	4 cut(s)	559, 670, 1187, 1673
EcoICRI	GAGCTC	1 cut(s)	1747
EcoRII	CCWGG	2 cut(s)	216, 504
EcoT14I	CCWWGG	2 cut(s)	1103, 1543
EcoT38I	GRGCYC	2 cut(s)	678, 1749
ErhI	CCWWGG	2 cut(s)	1103, 1543
Esp3I	CGTCTC	2 cut(s)	601, 1899
FaeI	CATG	2 cut(s)	1479, 1547
FalI	AAGNNNNNCTT	2 cut(s)	365, 397
FaqI	GGGAC	6 cut(s)	361, 465, 622, 1219, 1519, 1753
FatI	CATG	2 cut(s)	1475, 1543
FauI	CCCGC	2 cut(s)	296, 1103
FauNDI	CATATG	1 cut(s)	1308
FriOI	GRGCYC	2 cut(s)	678, 1749
FspBI	CTAG	1 cut(s)	1449
GlaI	GCGC	1 cut(s)	179
GsaI	CCCAGC	1 cut(s)	773
GsuI	CTGGAG	5 cut(s)	227, 347, 509, 515, 1175
HaeII	RGCGCY	1 cut(s)	181
HaeIII	GGCC	8 cut(s)	222, 339, 504, 618, 1509, 1592, 1611, 1909
HhaI	GCGC	1 cut(s)	180
Hin1I	GRCGYC	8 cut(s)	108, 204, 758, 843, 855, 1263, 1892, 1980
Hin1II	CATG	2 cut(s)	1479, 1547
Hin6I	GCGC	1 cut(s)	178
HinP1I	GCGC	1 cut(s)	178
HinfI	GANTC	4 cut(s)	4, 728, 1652, 1678
HphI	GGTGA	8 cut(s)	436, 508, 960, 1261, 1363, 1399, 1514, 1855
Hpy166II	GTNNAC	3 cut(s)	46, 694, 1760
Hpy188III	TCNNGA	8 cut(s)	275, 364, 488, 1154, 1189, 1649, 1750, 1898
Hpy8I	GTNNAC	3 cut(s)	46, 694, 1760
HpyCH4III	ACNGT	8 cut(s)	448, 691, 789, 954, 1072, 1151, 1249, 1436
HpyCH4IV	ACGT	7 cut(s)	594, 1211, 1314, 1703, 1797, 1892, 1980
HpyCH4V	TGCA	6 cut(s)	705, 785, 1048, 1465, 1637, 1658
HpyF10VI	GCNNNNNNNGC	7 cut(s)	210, 219, 231, 312, 549, 1622, 1786
HpyF3I	CTNAG	3 cut(s)	311, 792, 1140
HpySE526I	ACGT	7 cut(s)	594, 1211, 1314, 1703, 1797, 1892, 1980
Hsp92I	GRCGYC	8 cut(s)	108, 204, 758, 843, 855, 1263, 1892, 1980
Hsp92II	CATG	2 cut(s)	1479, 1547
HspAI	GCGC	1 cut(s)	178
Kpn2I	TCCGGA	1 cut(s)	1897
KroI	GCCGGC	2 cut(s)	1265, 1909
KroNI	GCCGGC	2 cut(s)	1267, 1911
Kzo9I	GATC	4 cut(s)	454, 1023, 1389, 1645
Lsp1109I	GCAGC	3 cut(s)	717, 1094, 1171
LweI	GCATC	2 cut(s)	1342, 1740
MaeI	CTAG	1 cut(s)	1449
MaeII	ACGT	7 cut(s)	594, 1211, 1314, 1703, 1797, 1892, 1980
MaeIII	GTNAC	1 cut(s)	1243
MalI	GATC	4 cut(s)	456, 1025, 1391, 1647
MbiI	CCGCTC	2 cut(s)	1110, 1381
MboI	GATC	4 cut(s)	454, 1023, 1389, 1645
MboII	GAAGA	8 cut(s)	373, 580, 715, 1264, 1495, 1505, 1655, 1942
MflI	RGATCY	2 cut(s)	1023, 1645
MhlI	GDGCHC	5 cut(s)	215, 678, 782, 1749, 1791
MluCI	AATT	3 cut(s)	960, 1000, 1098
MluI	ACGCGT	1 cut(s)	648
MmeI	TCCRAC	5 cut(s)	129, 561, 741, 1080, 1963
MroI	TCCGGA	1 cut(s)	1897
MroNI	GCCGGC	2 cut(s)	1265, 1909
MseI	TTAA	3 cut(s)	584, 603, 1947
MslI	CAYNNNNRTG	1 cut(s)	1796
MspA1I	CMGCKG	3 cut(s)	769, 1162, 1237
MspR9I	CCNGG	9 cut(s)	218, 506, 560, 561, 620, 640, 671, 672, 698
Mva1269I	GAATGC	2 cut(s)	981, 1863
MvaI	CCWGG	2 cut(s)	218, 506
MvnI	CGCG	3 cut(s)	225, 306, 650
MwoI	GCNNNNNNNGC	7 cut(s)	210, 219, 231, 312, 549, 1622, 1786
NaeI	GCCGGC	2 cut(s)	1267, 1911
NciI	CCSGG	7 cut(s)	560, 561, 620, 640, 671, 672, 698
NcoI	CCATGG	1 cut(s)	1543
NdeI	CATATG	1 cut(s)	1308
NdeII	GATC	4 cut(s)	454, 1023, 1389, 1645
NgoMIV	GCCGGC	2 cut(s)	1265, 1909
NlaIII	CATG	2 cut(s)	1479, 1547
NlaIV	GGNNCC	7 cut(s)	265, 340, 481, 553, 624, 765, 1347
NmeAIII	GCCGAG	2 cut(s)	831, 1154
NmuCI	GTSAC	1 cut(s)	1243
NspV	TTCGAA	2 cut(s)	726, 1439
OliI	CACNNNNGTG	1 cut(s)	1796
PasI	CCCWGGG	1 cut(s)	217
PceI	AGGCCT	2 cut(s)	1509, 1592
PcsI	WCGNNNNNNNCGW	4 cut(s)	39, 180, 234, 885
PctI	GAATGC	2 cut(s)	981, 1863
PdiI	GCCGGC	2 cut(s)	1267, 1911
PfeI	GAWTC	4 cut(s)	4, 728, 1652, 1678
PflFI	GACNNNGTC	1 cut(s)	110
Ppu21I	YACGTR	1 cut(s)	1212
Psp124BI	GAGCTC	1 cut(s)	1749
Psp6I	CCWGG	2 cut(s)	216, 504
PspFI	CCCAGC	1 cut(s)	769
PspGI	CCWGG	2 cut(s)	216, 504
PspN4I	GGNNCC	7 cut(s)	265, 340, 481, 553, 624, 765, 1347
PspPI	GGNCC	5 cut(s)	220, 338, 479, 508, 617
PstI	CTGCAG	1 cut(s)	1660
PsuI	RGATCY	2 cut(s)	1023, 1645
PsyI	GACNNNGTC	1 cut(s)	110
PvuII	CAGCTG	2 cut(s)	1162, 1237
RsaI	GTAC	1 cut(s)	1214
RsaNI	GTAC	1 cut(s)	1213
RseI	CAYNNNNRTG	1 cut(s)	1796
SacI	GAGCTC	1 cut(s)	1749
SaqAI	TTAA	3 cut(s)	584, 603, 1947
Sau3AI	GATC	4 cut(s)	454, 1023, 1389, 1645
Sau96I	GGNCC	5 cut(s)	220, 338, 479, 508, 617
ScrFI	CCNGG	9 cut(s)	218, 506, 560, 561, 620, 640, 671, 672, 698
SduI	GDGCHC	5 cut(s)	215, 678, 782, 1749, 1791
SfaNI	GCATC	2 cut(s)	1342, 1740
SfcI	CTRYAG	1 cut(s)	1656
SfuI	TTCGAA	2 cut(s)	726, 1439
SinI	GGWCC	2 cut(s)	479, 508
SmaI	CCCGGG	2 cut(s)	561, 672
SmiMI	CAYNNNNRTG	1 cut(s)	1796
SmlI	CTYRAG	4 cut(s)	371, 1503, 1553, 1742
SmoI	CTYRAG	4 cut(s)	371, 1503, 1553, 1742
SnaBI	TACGTA	1 cut(s)	1212
Sse9I	AATT	3 cut(s)	960, 1000, 1098
SseBI	AGGCCT	2 cut(s)	1509, 1592
SspMI	CTAG	1 cut(s)	1449
SstI	GAGCTC	1 cut(s)	1749
StuI	AGGCCT	2 cut(s)	1509, 1592
StyD4I	CCNGG	9 cut(s)	216, 504, 558, 559, 618, 638, 669, 670, 696
StyI	CCWWGG	2 cut(s)	1103, 1543
TaaI	ACNGT	8 cut(s)	448, 691, 789, 954, 1072, 1151, 1249, 1436
TaiI	ACGT	7 cut(s)	597, 1214, 1317, 1706, 1800, 1895, 1983
TaqI	TCGA	9 cut(s)	183, 294, 453, 645, 726, 913, 1014, 1439, 1587
TasI	AATT	3 cut(s)	960, 1000, 1098
TauI	GCSGC	7 cut(s)	79, 225, 684, 769, 887, 1333, 1384
TfiI	GAWTC	4 cut(s)	4, 728, 1652, 1678
Tru1I	TTAA	3 cut(s)	584, 603, 1947
Tru9I	TTAA	3 cut(s)	584, 603, 1947
TscAI	CASTG	1 cut(s)	787
TseFI	GTSAC	1 cut(s)	1243
TseI	GCWGC	3 cut(s)	705, 1082, 1159
Tsp45I	GTSAC	1 cut(s)	1243
TspDTI	ATGAA	1 cut(s)	1656
TspGWI	ACGGA	6 cut(s)	248, 1048, 1326, 1616, 1755, 1808
TspMI	CCCGGG	2 cut(s)	559, 670
TspRI	CASTG	1 cut(s)	787
Tth111I	GACNNNGTC	1 cut(s)	110
VpaK11BI	GGWCC	2 cut(s)	479, 508
XcmI	CCANNNNNNNNNTGG	1 cut(s)	836
XmaI	CCCGGG	2 cut(s)	559, 670
XspI	CTAG	1 cut(s)	1449
ZraI	GACGTC	2 cut(s)	1893, 1981

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.