Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009275

Physical Location & Seq

Genomic Coordinates Forward (+)

7984592 .. 7985608

Gene Structure 1017 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1095300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 633 bp

ATGTCTCGATTTTTTCTTCCTTCTCTTTTGGCTGGAAAATCAACTAGGGCAAAGCCGCTGCACTGGACGTCATGCTTGAAAATAGCAGAGGACATAGCACAAGGCCTCTCTTACATCCACCAAGCATGGAGACTTGTCCATGGCAATCTCAAGTCCACTAACGTCCTACTTGGCTCCGACTTCGAAGCATGTCTCACCGACTATTGCCTCTCCGTTCTCGCCACAACTACCCCAACGTCTGAGGAGGATCCAGATTCAGCTGCATACAAAGCCCCAGAGACTCGCACGAACTCATCAAATGATCACGATCATCATGATCAACAGCAGCAACCAACTTCAAAGTCCGATGTCTATGCATTTGGGATTTTGTTGGTGGAGCTTCTCACGGGGAAGCCTCCATCGCAGCACTTGGTTTTGCCGCCCAATGATACGATGAAATGGGTAAGATCCTTGAGGGAAGATGAGCAAAATGATGGGCATGATAAGATGGCAATGCTGCTGGAGGTGGCGATCGCTTGCAGTTCAACTTCGCCGGAGCAGAGGCCGACTATGTGGCAGGTGTTGAAGATGTTACAGGAGATTAAAGATGAGACAATATCATCCATGGAGGAGGTTGAAAATGAAGTGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

211

Amino Acids

23.48

Weight (kDa)

5.02

Isoelectric Point (pI)

51.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	17 - 190	5e-20	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	18 - 189	8.2e-17	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50230 AT3G50230 AT5G67200
fragaria_vesca	FvH4_1g16410
malus_domestica	MD02G1176600.v1.1 MD08G1095300.v1.1
prunus_persica	Prupe.6G231100_v2.0.a1
pyrus_communis	pycom01g08530 pycom02g14290 pycom15g07270
rosa_chinensis	RchiOBHm_Chr2g0106291
rosa_laevigata	RLG00000017486
rosa_multiflora	Rmu_sc0020846.1_g000001 Rmu_sc0021309.1_g000001 Rmu_ssc0000338.1_g000005
rosa_roxburghii	Rroxscaffold_2G00137300
rosa_rugosa	Rorug02G0134500
rosa_samantha	Rh2AG185300 Rh2BG195600 Rh2CG190400 Rh2DG191500
rosa_wichuraiana	Rw2G014470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	547
AatII	GACGTC	1 cut(s)	71
Acc36I	ACCTGC	1 cut(s)	547
AciI	CCGC	2 cut(s)	56, 419
AclWI	GGATC	3 cut(s)	242, 255, 441
AcyI	GRCGYC	1 cut(s)	68
AgsI	TTSAA	5 cut(s)	79, 339, 525, 565, 617
AluBI	AGCT	2 cut(s)	260, 379
AluI	AGCT	2 cut(s)	260, 379
Alw26I	GTCTC	5 cut(s)	9, 124, 197, 272, 584
AlwI	GGATC	3 cut(s)	242, 255, 441
AoxI	GGCC	2 cut(s)	103, 542
ApeKI	GCWGC	5 cut(s)	58, 260, 325, 403, 496
AsiSI	GCGATCGC	1 cut(s)	513
AsuHPI	GGTGA	1 cut(s)	187
AsuII	TTCGAA	1 cut(s)	183
BamHI	GGATCC	1 cut(s)	247
BbvI	GCAGC	5 cut(s)	45, 247, 337, 415, 483
BccI	CCATC	3 cut(s)	406, 467, 481
BcgI	CGANNNNNNTGC	2 cut(s)	335, 369
BclI	TGATCA	2 cut(s)	301, 316
BcoDI	GTCTC	5 cut(s)	9, 124, 197, 272, 584
BfaI	CTAG	1 cut(s)	45
BfuAI	ACCTGC	1 cut(s)	547
BisI	GCNGC	7 cut(s)	56, 59, 261, 326, 404, 419, 497
BlsI	GCNGC	7 cut(s)	57, 60, 262, 327, 405, 420, 498
BmiI	GGNNCC	2 cut(s)	175, 249
BpmI	CTGGAG	1 cut(s)	521
Bpu14I	TTCGAA	1 cut(s)	183
BpuEI	CTTGAG	2 cut(s)	134, 472
BsaBI	GATNNNNATC	1 cut(s)	306
BsaHI	GRCGYC	1 cut(s)	68
BsaJI	CCNNGG	2 cut(s)	139, 603
Bse1I	ACTGG	1 cut(s)	68
Bse3DI	GCAATG	1 cut(s)	498
Bse8I	GATNNNNATC	1 cut(s)	306
BseDI	CCNNGG	2 cut(s)	139, 603
BseGI	GGATG	2 cut(s)	114, 599
BseJI	GATNNNNATC	1 cut(s)	306
BseMI	GCAATG	1 cut(s)	498
BseMII	CTCAG	1 cut(s)	231
BseNI	ACTGG	1 cut(s)	68
BseRI	GAGGAG	2 cut(s)	257, 623
BseXI	GCAGC	5 cut(s)	45, 247, 337, 415, 483
BsgI	GTGCAG	1 cut(s)	44
Bsh1285I	CGRYCG	1 cut(s)	513
BshFI	GGCC	2 cut(s)	105, 544
BsiEI	CGRYCG	1 cut(s)	513
BsiSI	CCGG	1 cut(s)	533
BsmAI	GTCTC	5 cut(s)	9, 124, 197, 272, 584
BsnI	GGCC	2 cut(s)	105, 544
Bsp119I	TTCGAA	1 cut(s)	183
Bsp143I	GATC	6 cut(s)	247, 301, 307, 316, 446, 510
Bsp19I	CCATGG	2 cut(s)	139, 603
BspACI	CCGC	2 cut(s)	56, 419
BspANI	GGCC	2 cut(s)	105, 544
BspCNI	CTCAG	1 cut(s)	232
BspHI	TCATGA	1 cut(s)	313
BspLI	GGNNCC	2 cut(s)	175, 249
BspMI	ACCTGC	1 cut(s)	547
BspPI	GGATC	3 cut(s)	242, 255, 441
BspT104I	TTCGAA	1 cut(s)	183
BsrDI	GCAATG	1 cut(s)	498
BsrI	ACTGG	1 cut(s)	68
BssECI	CCNNGG	2 cut(s)	139, 603
BssMI	GATC	6 cut(s)	247, 301, 307, 316, 446, 510
BssNI	GRCGYC	1 cut(s)	68
BssT1I	CCWWGG	2 cut(s)	139, 603
BstACI	GRCGYC	1 cut(s)	68
BstBI	TTCGAA	1 cut(s)	183
BstC8I	GCNNGC	1 cut(s)	517
BstDEI	CTNAG	1 cut(s)	240
BstDSI	CCRYGG	2 cut(s)	139, 603
BstF5I	GGATG	2 cut(s)	114, 599
BstKTI	GATC	6 cut(s)	250, 304, 310, 319, 449, 513
BstMAI	GTCTC	5 cut(s)	9, 124, 197, 272, 584
BstMBI	GATC	6 cut(s)	247, 301, 307, 316, 446, 510
BstMCI	CGRYCG	1 cut(s)	513
BstMWI	GCNNNNNNNGC	2 cut(s)	269, 400
BstNSI	RCATGY	1 cut(s)	192
BstV1I	GCAGC	5 cut(s)	45, 247, 337, 415, 483
BstX2I	RGATCY	2 cut(s)	247, 446
BstYI	RGATCY	2 cut(s)	247, 446
BsuRI	GGCC	2 cut(s)	105, 544
BtgI	CCRYGG	2 cut(s)	139, 603
BtgZI	GCGATG	1 cut(s)	384
BtsCI	GGATG	2 cut(s)	114, 599
BtsIMutI	CAGTG	1 cut(s)	61
BveI	ACCTGC	1 cut(s)	547
Cac8I	GCNNGC	1 cut(s)	517
CciI	TCATGA	1 cut(s)	313
CviAII	CATG	7 cut(s)	72, 126, 140, 189, 314, 479, 604
CviJI	RGCY	9 cut(s)	32, 55, 105, 174, 260, 272, 379, 394, 544
CviKI_1	RGCY	9 cut(s)	32, 55, 105, 174, 260, 272, 379, 394, 544
DdeI	CTNAG	1 cut(s)	240
DpnI	GATC	6 cut(s)	249, 303, 309, 318, 448, 512
DpnII	GATC	6 cut(s)	247, 301, 307, 316, 446, 510
Eco130I	CCWWGG	2 cut(s)	139, 603
Eco147I	AGGCCT	1 cut(s)	105
EcoT14I	CCWWGG	2 cut(s)	139, 603
EcoT22I	ATGCAT	1 cut(s)	358
ErhI	CCWWGG	2 cut(s)	139, 603
FaeI	CATG	7 cut(s)	75, 129, 143, 192, 317, 482, 607
FatI	CATG	7 cut(s)	71, 125, 139, 188, 313, 478, 603
FbaI	TGATCA	2 cut(s)	301, 316
Fnu4HI	GCNGC	7 cut(s)	56, 59, 261, 326, 404, 419, 497
FokI	GGATG	2 cut(s)	101, 586
Fsp4HI	GCNGC	7 cut(s)	56, 59, 261, 326, 404, 419, 497
FspBI	CTAG	1 cut(s)	45
GluI	GCNGC	7 cut(s)	56, 59, 261, 326, 404, 419, 497
GsuI	CTGGAG	1 cut(s)	521
HaeIII	GGCC	2 cut(s)	105, 544
HapII	CCGG	1 cut(s)	533
Hin1I	GRCGYC	1 cut(s)	68
Hin1II	CATG	7 cut(s)	75, 129, 143, 192, 317, 482, 607
HinfI	GANTC	2 cut(s)	254, 280
HpaII	CCGG	1 cut(s)	533
HphI	GGTGA	1 cut(s)	187
Hpy166II	GTNNAC	1 cut(s)	156
Hpy188I	TCNGA	3 cut(s)	178, 241, 346
Hpy188III	TCNNGA	4 cut(s)	6, 251, 305, 314
Hpy8I	GTNNAC	1 cut(s)	156
HpyAV	CCTTC	1 cut(s)	30
HpyCH4IV	ACGT	3 cut(s)	68, 162, 236
HpyCH4V	TGCA	4 cut(s)	61, 263, 356, 519
HpyF10VI	GCNNNNNNNGC	2 cut(s)	269, 400
HpyF3I	CTNAG	1 cut(s)	240
HpySE526I	ACGT	3 cut(s)	68, 162, 236
Hsp92I	GRCGYC	1 cut(s)	68
Hsp92II	CATG	7 cut(s)	75, 129, 143, 192, 317, 482, 607
Ksp22I	TGATCA	2 cut(s)	301, 316
Kzo9I	GATC	6 cut(s)	247, 301, 307, 316, 446, 510
LmnI	GCTCC	3 cut(s)	179, 376, 535
LpnPI	CCDG	8 cut(s)	18, 49, 264, 288, 485, 542, 546, 560
Lsp1109I	GCAGC	5 cut(s)	45, 247, 337, 415, 483
MaeI	CTAG	1 cut(s)	45
MaeII	ACGT	3 cut(s)	68, 162, 236
MaeIII	GTNAC	1 cut(s)	570
MalI	GATC	6 cut(s)	249, 303, 309, 318, 448, 512
MboI	GATC	6 cut(s)	247, 301, 307, 316, 446, 510
MboII	GAAGA	3 cut(s)	8, 470, 577
MflI	RGATCY	2 cut(s)	247, 446
MlyI	GAGTC	1 cut(s)	274
MmeI	TCCRAC	1 cut(s)	201
Mph1103I	ATGCAT	1 cut(s)	358
MseI	TTAA	1 cut(s)	582
MspA1I	CMGCKG	2 cut(s)	58, 260
MspI	CCGG	1 cut(s)	533
MwoI	GCNNNNNNNGC	2 cut(s)	269, 400
NcoI	CCATGG	2 cut(s)	139, 603
NdeII	GATC	6 cut(s)	247, 301, 307, 316, 446, 510
NlaIII	CATG	7 cut(s)	75, 129, 143, 192, 317, 482, 607
NlaIV	GGNNCC	2 cut(s)	175, 249
NsiI	ATGCAT	1 cut(s)	358
NspI	RCATGY	1 cut(s)	192
NspV	TTCGAA	1 cut(s)	183
PagI	TCATGA	1 cut(s)	313
PaqCI	CACCTGC	1 cut(s)	547
PceI	AGGCCT	1 cut(s)	105
PfeI	GAWTC	1 cut(s)	254
PkrI	GCNGC	7 cut(s)	57, 60, 262, 327, 405, 420, 498
Ple19I	CGATCG	1 cut(s)	513
PleI	GAGTC	1 cut(s)	274
PpsI	GAGTC	1 cut(s)	274
PspN4I	GGNNCC	2 cut(s)	175, 249
PsuI	RGATCY	2 cut(s)	247, 446
PvuI	CGATCG	1 cut(s)	513
PvuII	CAGCTG	1 cut(s)	260
RgaI	GCGATCGC	1 cut(s)	513
SaqAI	TTAA	1 cut(s)	582
SatI	GCNGC	7 cut(s)	56, 59, 261, 326, 404, 419, 497
Sau3AI	GATC	6 cut(s)	247, 301, 307, 316, 446, 510
SchI	GAGTC	1 cut(s)	274
SetI	ASST	8 cut(s)	71, 165, 239, 262, 381, 507, 561, 615
SfaAI	GCGATCGC	1 cut(s)	513
SfuI	TTCGAA	1 cut(s)	183
SgfI	GCGATCGC	1 cut(s)	513
SmlI	CTYRAG	2 cut(s)	149, 451
SmoI	CTYRAG	2 cut(s)	149, 451
SseBI	AGGCCT	1 cut(s)	105
SsiI	CCGC	2 cut(s)	56, 419
SspMI	CTAG	1 cut(s)	45
StuI	AGGCCT	1 cut(s)	105
StyI	CCWWGG	2 cut(s)	139, 603
TaiI	ACGT	3 cut(s)	71, 165, 239
TaqI	TCGA	2 cut(s)	7, 183
TauI	GCSGC	2 cut(s)	58, 421
TfiI	GAWTC	1 cut(s)	254
Tru1I	TTAA	1 cut(s)	582
Tru9I	TTAA	1 cut(s)	582
TscAI	CASTG	1 cut(s)	68
TseI	GCWGC	5 cut(s)	58, 260, 325, 403, 496
TspDTI	ATGAA	1 cut(s)	449
TspGWI	ACGGA	1 cut(s)	202
TspRI	CASTG	1 cut(s)	68
XceI	RCATGY	1 cut(s)	192
XspI	CTAG	1 cut(s)	45
ZraI	GACGTC	1 cut(s)	69
Zsp2I	ATGCAT	1 cut(s)	358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.