AT3G54380

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003610
Physical Location & Seq
Reverse (-)
20133893 .. 20137007
3115 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54380.2

Sequence Viewer

Length: 1221 bp
ATGAATCGTCGTAATCGTGGCTCTTCTTCTTCTTCTTCTAGGGTTTCGAATACTTATGGGAACCGCCAGTTCTCTGATAACCCGAGGACTGGATCCGGCGGCGGAGTTAATGAATCCTTTCAGAGAAGATCCGATGCACCGCACAAACGAAACAATGAAAAAGACGAATCAAAACATAAAGACGAAGATCCTGCTGACGTTTCATTGATTGTAGGAACTTGTTCTTCTATGTGCCCAGAGAGGGAAAGAGTCACAAGAGAACGCCTGCGTGATCTTGCAGTGTTTGAGAGGCTTTATGGAAACCCCTCAAAATCATCCACAGAGATAGCTGTCAAAAAGTTTTGCAGAACCCTTTCAGCGGCGGATGTTCAGGCATCGGATGTAAGACCGCTCCCAGTTTTAGAAGAAACCCTGAGATACCTTCTAAGTTTGCTCGACTCCAAGGAGCACCCGTTTGAAGTGGTTCATGATTTTATTTTCGATAGGACAAGGTCTATTAGACAAGATCTTAGCATCCAGAATTTGGCCAATGAGAGAGTGATCTACCTTTATGAGGAAATGGTAAAATTTCATGTCATATCTCACGAGAGGCTTCAAAGTTGTAGTGGTACAAGTATTAGCTCAATGCATCACTTGAACATGGAGCAGCTAGCAAAAACTCTTACCTCTTTGTATAATATATATGATGCAAATAGAAAACCGGACTATATCTACGAAAATGAAGCCGAGTTCCGCTCACTCTATGTGCTGCTTCATCTCAATCCTAGTAGTGGAGTGATGGGGGAACCGCTGTCTTTATGGTTTCGCAAGTTGACTTTTGCTTTGGTAAAATCCAAGGAAATATGTTTTGTACGAAACCTTTTAAGATTATATCGGATGGGCAATTACAAGAACTTCTTAAGTAGAACAGCTTCTGAGGCGACATACTTGCAGTATTGCATAAGTGAACATCATATTAGAGAGATGCGATTAGTGGCTGTCCAATACATAAACAATGTTTGCTACAAGCTTCAACCCTATCCTCTCTTGCGGTTATCTCAGAACCTGAAGATGAAGGAGTTGGATGTTGAATCTTTATGCCATGAGTGTGGTCTTGAGACATGTACTGATCCTGATGGGTTCACTGTTTTACCCGTGAAGCAGTCAACCTTTCGTAGTCCAGAGGACAAGTTCAAAGTTTATGATTTGATTGGAATTGAACGGATTAAGATGTCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

406

Amino Acids

46.97

Weight (kDa)

8.56

Isoelectric Point (pI)

54.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 8 - 279 4.6e-68 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 523
AccBSI CCGCTC 2 cut(s) 391, 735
AclWI GGATC 5 cut(s) 87, 100, 123, 182, 1103
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 520, 566
AcuI CTGAAG 1 cut(s) 1067
AfaI GTAC 3 cut(s) 610, 852, 1105
AfiI CCNNNNNNNGG 6 cut(s) 89, 241, 358, 523, 553, 770
AflII CTTAAG 1 cut(s) 898
AflIII ACRYGT 1 cut(s) 1100
AgsI TTSAA 7 cut(s) 458, 596, 637, 1013, 1070, 1174, 1199
AluBI AGCT 5 cut(s) 329, 621, 649, 911, 1009
AluI AGCT 5 cut(s) 329, 621, 649, 911, 1009
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 1 cut(s) 1091
AlwI GGATC 5 cut(s) 87, 100, 123, 182, 1103
AlwNI CAGNNNCTG 2 cut(s) 914, 1045
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 1 cut(s) 525
ApeKI GCWGC 2 cut(s) 646, 748
ApoI RAATTY 2 cut(s) 520, 566
ArsI GACNNNNNNTTYG 2 cut(s) 805, 837
Asp700I GAANNNNTTC 5 cut(s) 117, 220, 352, 462, 910
AsuII TTCGAA 1 cut(s) 47
AsuNHI GCTAGC 1 cut(s) 649
AvaI CYCGRG 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 236
BalI TGGCCA 1 cut(s) 527
BamHI GGATCC 1 cut(s) 92
BarI GAAGNNNNNNTAC 2 cut(s) 895, 927
BauI CACGAG 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 2 cut(s) 658, 735
BccI CCATC 3 cut(s) 772, 871, 1109
BcgI CGANNNNNNTGC 4 cut(s) 173, 207, 910, 944
BcoDI GTCTC 1 cut(s) 1091
BfaI CTAG 3 cut(s) 39, 650, 765
BfrI CTTAAG 1 cut(s) 898
BglII AGATCT 1 cut(s) 505
BisI GCNGC 4 cut(s) 100, 360, 647, 749
BlsI GCNGC 4 cut(s) 101, 361, 648, 750
BmeT110I CYCGRG 1 cut(s) 82
BmiI GGNNCC 3 cut(s) 62, 94, 786
BmrI ACTGGG 1 cut(s) 389
BmsI GCATC 6 cut(s) 124, 383, 522, 637, 676, 954
BmtI GCTAGC 1 cut(s) 653
BmuI ACTGGG 1 cut(s) 389
BplI GAGNNNNNCTC 2 cut(s) 719, 751
Bpu14I TTCGAA 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 1115
BsaJI CCNNGG 3 cut(s) 83, 441, 834
BsaWI WCCGGW 1 cut(s) 700
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 6 cut(s) 89, 241, 358, 523, 553, 770
Bse1I ACTGG 3 cut(s) 67, 94, 395
BseDI CCNNGG 3 cut(s) 83, 441, 834
BseGI GGATG 6 cut(s) 314, 370, 385, 513, 882, 1069
BseLI CCNNNNNNNGG 6 cut(s) 89, 241, 358, 523, 553, 770
BseMII CTCAG 3 cut(s) 404, 906, 1052
BseNI ACTGG 3 cut(s) 67, 94, 395
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 2 cut(s) 658, 735
BshFI GGCC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 2 cut(s) 96, 701
BslI CCNNNNNNNGG 6 cut(s) 89, 241, 358, 523, 553, 770
BsmAI GTCTC 1 cut(s) 1091
BsnI GGCC 1 cut(s) 527
BsoBI CYCGRG 1 cut(s) 82
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 236, 450
Bsp143I GATC 7 cut(s) 92, 128, 187, 271, 505, 540, 1108
BspANI GGCC 1 cut(s) 527
BspCNI CTCAG 3 cut(s) 405, 907, 1051
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 3 cut(s) 62, 94, 786
BspOI GCTAGC 1 cut(s) 653
BspPI GGATC 5 cut(s) 87, 100, 123, 182, 1103
BspQI GCTCTTC 1 cut(s) 28
BspT104I TTCGAA 1 cut(s) 47
BspTI CTTAAG 1 cut(s) 898
BsrBI CCGCTC 2 cut(s) 391, 735
BsrI ACTGG 3 cut(s) 67, 94, 395
BssECI CCNNGG 3 cut(s) 83, 441, 834
BssMI GATC 7 cut(s) 92, 128, 187, 271, 505, 540, 1108
BssSI CACGAG 1 cut(s) 584
BssT1I CCWWGG 2 cut(s) 441, 834
Bst2BI CACGAG 1 cut(s) 584
Bst4CI ACNGT 1 cut(s) 1126
Bst6I CTCTTC 1 cut(s) 28
BstAFI CTTAAG 1 cut(s) 898
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 2 cut(s) 266, 651
BstDEI CTNAG 5 cut(s) 413, 425, 509, 915, 1038
BstENI CCTNNNNNAGG 1 cut(s) 551
BstF5I GGATG 6 cut(s) 314, 370, 385, 513, 882, 1069
BstKTI GATC 7 cut(s) 95, 131, 190, 274, 508, 543, 1111
BstMAI GTCTC 1 cut(s) 1091
BstMBI GATC 7 cut(s) 92, 128, 187, 271, 505, 540, 1108
BstMWI GCNNNNNNNGC 1 cut(s) 917
BstNSI RCATGY 1 cut(s) 1104
BstSLI GKGCMC 1 cut(s) 236
BstV1I GCAGC 2 cut(s) 658, 735
BstX2I RGATCY 4 cut(s) 92, 128, 187, 505
BstXI CCANNNNNNTGG 1 cut(s) 1088
BstYI RGATCY 4 cut(s) 92, 128, 187, 505
BsuRI GGCC 1 cut(s) 527
BtsCI GGATG 6 cut(s) 314, 370, 385, 513, 882, 1069
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 2 cut(s) 285, 1122
Cac8I GCNNGC 2 cut(s) 266, 651
CaiI CAGNNNCTG 2 cut(s) 914, 1045
CciI TCATGA 1 cut(s) 466
Csp6I GTAC 3 cut(s) 609, 851, 1104
CviAII CATG 5 cut(s) 467, 572, 640, 1082, 1101
CviQI GTAC 3 cut(s) 609, 851, 1104
DdeI CTNAG 5 cut(s) 413, 425, 509, 915, 1038
DpnI GATC 7 cut(s) 94, 130, 189, 273, 507, 542, 1110
DpnII GATC 7 cut(s) 92, 128, 187, 271, 505, 540, 1108
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
EciI GGCGGA 2 cut(s) 117, 377
Eco130I CCWWGG 2 cut(s) 441, 834
Eco57I CTGAAG 1 cut(s) 1067
Eco88I CYCGRG 1 cut(s) 82
EcoNI CCTNNNNNAGG 1 cut(s) 551
EcoT14I CCWWGG 2 cut(s) 441, 834
EcoT22I ATGCAT 1 cut(s) 630
ErhI CCWWGG 2 cut(s) 441, 834
FaeI CATG 5 cut(s) 470, 575, 643, 1085, 1104
FalI AAGNNNNNCTT 2 cut(s) 881, 913
FatI CATG 5 cut(s) 466, 571, 639, 1081, 1100
Fnu4HI GCNGC 4 cut(s) 100, 360, 647, 749
FokI GGATG 6 cut(s) 301, 377, 392, 500, 889, 1076
Fsp4HI GCNGC 4 cut(s) 100, 360, 647, 749
FspBI CTAG 3 cut(s) 39, 650, 765
GluI GCNGC 4 cut(s) 100, 360, 647, 749
HaeIII GGCC 1 cut(s) 527
HapII CCGG 2 cut(s) 96, 701
Hin1II CATG 5 cut(s) 470, 575, 643, 1085, 1104
HincII GTYRAC 2 cut(s) 813, 1146
HindII GTYRAC 2 cut(s) 813, 1146
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 6 cut(s) 4, 113, 167, 249, 437, 1070
HpaII CCGG 2 cut(s) 96, 701
Hpy166II GTNNAC 4 cut(s) 813, 947, 1122, 1146
Hpy188I TCNGA 7 cut(s) 76, 123, 133, 379, 876, 916, 1041
Hpy188III TCNNGA 6 cut(s) 467, 517, 584, 1094, 1112, 1160
Hpy8I GTNNAC 4 cut(s) 813, 947, 1122, 1146
Hpy99I CGWCG 1 cut(s) 12
HpyAV CCTTC 2 cut(s) 431, 1048
HpyCH4III ACNGT 1 cut(s) 1126
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 7 cut(s) 137, 278, 345, 628, 689, 931, 939
HpyF10VI GCNNNNNNNGC 1 cut(s) 917
HpyF3I CTNAG 5 cut(s) 413, 425, 509, 915, 1038
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 5 cut(s) 470, 575, 643, 1085, 1104
Kzo9I GATC 7 cut(s) 92, 128, 187, 271, 505, 540, 1108
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 3 cut(s) 396, 445, 643
Lsp1109I GCAGC 2 cut(s) 658, 735
LweI GCATC 6 cut(s) 124, 383, 522, 637, 676, 954
MaeI CTAG 3 cut(s) 39, 650, 765
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 250
MalI GATC 7 cut(s) 94, 130, 189, 273, 507, 542, 1110
MbiI CCGCTC 2 cut(s) 391, 735
MboI GATC 7 cut(s) 92, 128, 187, 271, 505, 540, 1108
MflI RGATCY 4 cut(s) 92, 128, 187, 505
MhlI GDGCHC 2 cut(s) 236, 450
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 4 cut(s) 520, 566, 883, 1194
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 2 cut(s) 258, 431
MmeI TCCRAC 1 cut(s) 1041
Mox20I TGGCCA 1 cut(s) 527
Mph1103I ATGCAT 1 cut(s) 630
MroXI GAANNNNTTC 5 cut(s) 117, 220, 352, 462, 910
MscI TGGCCA 1 cut(s) 527
MseI TTAA 4 cut(s) 108, 863, 899, 1206
MslI CAYNNNNRTG 1 cut(s) 1086
Msp20I TGGCCA 1 cut(s) 527
MspA1I CMGCKG 2 cut(s) 359, 790
MspCI CTTAAG 1 cut(s) 898
MspI CCGG 2 cut(s) 96, 701
MwoI GCNNNNNNNGC 1 cut(s) 917
NdeII GATC 7 cut(s) 92, 128, 187, 271, 505, 540, 1108
NheI GCTAGC 1 cut(s) 649
NlaIII CATG 5 cut(s) 470, 575, 643, 1085, 1104
NlaIV GGNNCC 3 cut(s) 62, 94, 786
NmeAIII GCCGAG 1 cut(s) 751
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 630
NspI RCATGY 1 cut(s) 1104
NspV TTCGAA 1 cut(s) 47
PagI TCATGA 1 cut(s) 466
PciI ACATGT 1 cut(s) 1100
PciSI GCTCTTC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 13
PdmI GAANNNNTTC 5 cut(s) 117, 220, 352, 462, 910
PfeI GAWTC 4 cut(s) 4, 113, 167, 1070
PflFI GACNNNGTC 1 cut(s) 490
PflMI CCANNNNNTGG 1 cut(s) 523
PkrI GCNGC 4 cut(s) 101, 361, 648, 750
PleI GAGTC 2 cut(s) 257, 431
PpsI GAGTC 2 cut(s) 257, 431
PscI ACATGT 1 cut(s) 1100
PspN4I GGNNCC 3 cut(s) 62, 94, 786
PstNI CAGNNNCTG 2 cut(s) 914, 1045
PsuI RGATCY 4 cut(s) 92, 128, 187, 505
PsyI GACNNNGTC 1 cut(s) 490
RsaI GTAC 3 cut(s) 610, 852, 1105
RsaNI GTAC 3 cut(s) 609, 851, 1104
RseI CAYNNNNRTG 1 cut(s) 1086
SapI GCTCTTC 1 cut(s) 28
SaqAI TTAA 4 cut(s) 108, 863, 899, 1206
SatI GCNGC 4 cut(s) 100, 360, 647, 749
Sau3AI GATC 7 cut(s) 92, 128, 187, 271, 505, 540, 1108
SchI GAGTC 2 cut(s) 258, 431
SduI GDGCHC 2 cut(s) 236, 450
SfaNI GCATC 6 cut(s) 124, 383, 522, 637, 676, 954
SfuI TTCGAA 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 1086
SmlI CTYRAG 2 cut(s) 898, 1094
SmoI CTYRAG 2 cut(s) 898, 1094
Sse9I AATT 4 cut(s) 520, 566, 883, 1194
SspMI CTAG 3 cut(s) 39, 650, 765
StyI CCWWGG 2 cut(s) 441, 834
TaaI ACNGT 1 cut(s) 1126
TaiI ACGT 1 cut(s) 201
TaqI TCGA 4 cut(s) 47, 435, 480, 1214
TasI AATT 4 cut(s) 520, 566, 883, 1194
TatI WGTACW 1 cut(s) 1103
TauI GCSGC 2 cut(s) 102, 362
TfiI GAWTC 4 cut(s) 4, 113, 167, 1070
Tru1I TTAA 4 cut(s) 108, 863, 899, 1206
Tru9I TTAA 4 cut(s) 108, 863, 899, 1206
TscAI CASTG 2 cut(s) 285, 1129
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 646, 748
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 9 cut(s) 17, 126, 171, 192, 455, 560, 735, 743, 1067
TspGWI ACGGA 1 cut(s) 1216
TspRI CASTG 2 cut(s) 285, 1129
Tth111I GACNNNGTC 1 cut(s) 490
Van91I CCANNNNNTGG 1 cut(s) 523
Vha464I CTTAAG 1 cut(s) 898
XagI CCTNNNNNAGG 1 cut(s) 551
XapI RAATTY 2 cut(s) 520, 566
XceI RCATGY 1 cut(s) 1104
XmnI GAANNNNTTC 5 cut(s) 117, 220, 352, 462, 910
XspI CTAG 3 cut(s) 39, 650, 765
Zsp2I ATGCAT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.