RchiOBHm_Chr1g0329461

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003610
Physical Location & Seq
Reverse (-)
19089392 .. 19097349
7958 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55877

Sequence Viewer

Length: 1134 bp
ATGGAGAGAAGAGGCCGTCGAAACCGTTCTTCTTCTTCTTCTTCTCGGGGTTCAAGATCCTGGAGTAGAGGTGGAACTAGTTCCGCCGAGAAAGTGGAAAACGTCGCCGTTTCCGATGTTCCCGCCATAGTGGGTACTTGCACTTTGATGTGCCCAGAAGCAGAAAGGGCACAGCGGGAGCGCCTTCGCGATTTAGCTACCTTTGAGAGGCTGAATGGAAATCCAGCTCGCTCATCGCCATCCATTGCAGTTAAAAAGTTTTTCAGAACTATGTCCACCAAACAAGTTCAAGCATCAGATGTTCGGCCGCTCCCTGTTTTGGAGGATACTTTAAATTACCTCTTAGAGTTGTTTGATTCTAAAGACTATCCTTTTGAAGTGATTCATGACTTCCTCTTTGATCGAACAAGGTCCATAAGGCAAGATCTTAGCATGCAGAATATTGGTGGCGAGAAAGCAATTCACATGTATCAGGAAATGGTTAAATTTCATATCATCTCTCTTCATAAGCTTCGAGGTTGCAGCAGTCAAAATACTTCTCCAATGCATCACCTCAACATGGAGCAGCTTGCAAAGACCTTGACATCTCTATTTAATCTGTATGGCGCAAATCGTCATTCTAATTCTACTAATGAAAATGAGGCTGAGTTTTGTTCTTTTTATGTGCTACTTCATCTTGGTTCTCATAGCCAACCCCTGGGGGAGTCACTTTCTTGGTGGTTTAGTCATGTGCCTTCTTCAATAATCAAGTCAAAGGAAATGCATTTTTCCCGAAAGATTTTACGATATTTTCGGATGGGTAACTATAAACGTTTCTTAAGTACTGTAGCAGCTGAGGCATCTTATCTTCAATACTGCATTATTGAGCCTTATGTTGATGAGGTTCGAGTTTTAGCTGTGTCATGCATCAATAGTGGTGGCTACAAGCTTCATCCATATCCTCTGTCGCATTTATCCAAACTCTTGATTATGAAGGAATCAGATTTGGAATCTTTTCTCAAATCCTGTGGTCTTCAAATTTGCACTGATGAAGAAGGACATAATCTGTTGCCCACTAAGCAAACGACCTTTTGTCAACCCAAGGATGGGTCTCAAAACTACAGGTTTTGGGGTATGGAACAATTTGATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

377

Amino Acids

43.2

Weight (kDa)

8.87

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 50 - 340 3.4e-80 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 697
AccBSI CCGCTC 1 cut(s) 310
AccII CGCG 1 cut(s) 189
AciI CCGC 4 cut(s) 84, 123, 175, 308
AclI AACGTT 1 cut(s) 811
AclWI GGATC 1 cut(s) 51
AcoI YGGCCR 1 cut(s) 305
AcsI RAATTY 2 cut(s) 485, 1017
AfaI GTAC 2 cut(s) 136, 823
AfiI CCNNNNNNNGG 6 cut(s) 207, 319, 559, 697, 1085, 1086
AflII CTTAAG 1 cut(s) 817
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 6 cut(s) 54, 290, 377, 741, 851, 1016
AhdI GACNNNNNGTC 1 cut(s) 1071
AhlI ACTAGT 1 cut(s) 77
AjnI CCWGG 2 cut(s) 59, 696
AluBI AGCT 7 cut(s) 197, 227, 511, 568, 833, 896, 928
AluI AGCT 7 cut(s) 197, 227, 511, 568, 833, 896, 928
Alw26I GTCTC 1 cut(s) 1095
AlwI GGATC 1 cut(s) 51
Ama87I CYCGRG 1 cut(s) 45
AoxI GGCC 2 cut(s) 13, 305
ApeKI GCWGC 3 cut(s) 522, 565, 830
ApoI RAATTY 2 cut(s) 485, 1017
ArsI GACNNNNNNTTYG 4 cut(s) 356, 380, 388, 412
Asp700I GAANNNNTTC 4 cut(s) 25, 79, 381, 993
AspLEI GCGC 2 cut(s) 183, 608
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 542
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 1 cut(s) 411
BaeGI GKGCMC 2 cut(s) 155, 172
BbsI GAAGAC 1 cut(s) 1004
BbvCI CCTCAGC 1 cut(s) 834
BbvI GCAGC 3 cut(s) 534, 577, 842
BccI CCATC 3 cut(s) 247, 790, 1079
BceAI ACGGC 1 cut(s) 92
BciT130I CCWGG 2 cut(s) 61, 698
BciVI GTATCC 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 1095
BcuI ACTAGT 1 cut(s) 77
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 2 cut(s) 825, 1099
BfoI RGCGCY 1 cut(s) 184
BfrI CTTAAG 1 cut(s) 817
BfuI GTATCC 1 cut(s) 319
BglII AGATCT 1 cut(s) 424
BisI GCNGC 4 cut(s) 308, 523, 566, 831
BlsI GCNGC 4 cut(s) 309, 524, 567, 832
BmcAI AGTACT 1 cut(s) 823
Bme1390I CCNGG 2 cut(s) 61, 698
Bme18I GGWCC 1 cut(s) 411
BmeRI GACNNNNNGTC 1 cut(s) 1071
BmeT110I CYCGRG 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 411
BmrFI CCNGG 2 cut(s) 61, 698
BmsI GCATC 4 cut(s) 302, 556, 848, 915
BpiI GAAGAC 1 cut(s) 1004
BpmI CTGGAG 1 cut(s) 82
Bpu10I CCTNAGC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 1095
BsaJI CCNNGG 3 cut(s) 696, 697, 1080
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 6 cut(s) 207, 319, 559, 697, 1085, 1086
Bse3DI GCAATG 1 cut(s) 243
BseBI CCWGG 2 cut(s) 61, 698
BseDI CCNNGG 3 cut(s) 696, 697, 1080
BseGI GGATG 4 cut(s) 239, 801, 931, 1090
BseLI CCNNNNNNNGG 6 cut(s) 207, 319, 559, 697, 1085, 1086
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 2 cut(s) 636, 825
BseSI GKGCMC 2 cut(s) 155, 172
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 3 cut(s) 534, 577, 842
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 2 cut(s) 15, 307
BsiEI CGRYCG 1 cut(s) 308
BsiHKCI CYCGRG 1 cut(s) 45
BslI CCNNNNNNNGG 6 cut(s) 207, 319, 559, 697, 1085, 1086
BsmAI GTCTC 1 cut(s) 1095
BsnI GGCC 2 cut(s) 15, 307
Bso31I GGTCTC 1 cut(s) 1095
BsoBI CYCGRG 1 cut(s) 45
Bsp1286I GDGCHC 2 cut(s) 155, 172
Bsp143I GATC 3 cut(s) 56, 400, 424
Bsp68I TCGCGA 1 cut(s) 189
BspACI CCGC 4 cut(s) 84, 123, 175, 308
BspANI GGCC 2 cut(s) 15, 307
BspCNI CTCAG 2 cut(s) 637, 826
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 385
BspPI GGATC 1 cut(s) 51
BspTI CTTAAG 1 cut(s) 817
BspTNI GGTCTC 1 cut(s) 1095
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 243
BssECI CCNNGG 3 cut(s) 696, 697, 1080
BssMI GATC 3 cut(s) 56, 400, 424
BssT1I CCWWGG 1 cut(s) 1080
Bst2UI CCWGG 2 cut(s) 61, 698
Bst4CI ACNGT 2 cut(s) 26, 826
Bst6I CTCTTC 2 cut(s) 4, 507
BstAFI CTTAAG 1 cut(s) 817
BstC8I GCNNGC 3 cut(s) 229, 434, 570
BstDEI CTNAG 5 cut(s) 343, 428, 645, 834, 1056
BstENI CCTNNNNNAGG 1 cut(s) 205
BstF5I GGATG 4 cut(s) 239, 801, 931, 1090
BstFNI CGCG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 2 cut(s) 183, 608
BstKTI GATC 3 cut(s) 59, 403, 427
BstMAI GTCTC 1 cut(s) 1095
BstMBI GATC 3 cut(s) 56, 400, 424
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 167, 836, 1057
BstNI CCWGG 2 cut(s) 61, 698
BstNSI RCATGY 2 cut(s) 436, 469
BstSCI CCNGG 2 cut(s) 59, 696
BstSFI CTRYAG 2 cut(s) 825, 1099
BstSLI GKGCMC 2 cut(s) 155, 172
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 3 cut(s) 534, 577, 842
BstV2I GAAGAC 1 cut(s) 1004
BstX2I RGATCY 2 cut(s) 56, 424
BstYI RGATCY 2 cut(s) 56, 424
BstZI CGGCCG 1 cut(s) 305
BsuI GTATCC 1 cut(s) 319
BsuRI GGCC 2 cut(s) 15, 307
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 4 cut(s) 239, 801, 931, 1090
BtsIMutI CAGTG 1 cut(s) 1023
BtuMI TCGCGA 1 cut(s) 189
Cac8I GCNNGC 3 cut(s) 229, 434, 570
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 183, 608
Cfr13I GGNCC 1 cut(s) 411
Csp6I GTAC 2 cut(s) 135, 822
CspCI CAANNNNNGTGG 4 cut(s) 898, 933, 988, 1023
CviAII CATG 6 cut(s) 386, 433, 466, 559, 728, 903
CviQI GTAC 2 cut(s) 135, 822
DdeI CTNAG 5 cut(s) 343, 428, 645, 834, 1056
DpnI GATC 3 cut(s) 58, 402, 426
DpnII GATC 3 cut(s) 56, 400, 424
DraI TTTAAA 1 cut(s) 333
DriI GACNNNNNGTC 1 cut(s) 1071
EaeI YGGCCR 1 cut(s) 305
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 2 cut(s) 4, 507
Eam1105I GACNNNNNGTC 1 cut(s) 1071
EarI CTCTTC 2 cut(s) 4, 507
EciI GGCGGA 1 cut(s) 73
EclXI CGGCCG 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 1080
Eco31I GGTCTC 1 cut(s) 1095
Eco47I GGWCC 1 cut(s) 411
Eco52I CGGCCG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 45
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 59, 696
EcoT14I CCWWGG 1 cut(s) 1080
EcoT22I ATGCAT 3 cut(s) 549, 765, 908
ErhI CCWWGG 1 cut(s) 1080
FaeI CATG 6 cut(s) 389, 436, 469, 562, 731, 906
FatI CATG 6 cut(s) 385, 432, 465, 558, 727, 902
FauI CCCGC 2 cut(s) 130, 168
Fnu4HI GCNGC 4 cut(s) 308, 523, 566, 831
FokI GGATG 4 cut(s) 226, 808, 918, 1097
Fsp4HI GCNGC 4 cut(s) 308, 523, 566, 831
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 182, 607
GluI GCNGC 4 cut(s) 308, 523, 566, 831
GsuI CTGGAG 1 cut(s) 82
HaeII RGCGCY 1 cut(s) 184
HaeIII GGCC 2 cut(s) 15, 307
HhaI GCGC 2 cut(s) 183, 608
Hin1II CATG 6 cut(s) 389, 436, 469, 562, 731, 906
Hin6I GCGC 2 cut(s) 181, 606
HinP1I GCGC 2 cut(s) 181, 606
HincII GTYRAC 1 cut(s) 1076
HindII GTYRAC 1 cut(s) 1076
HindIII AAGCTT 2 cut(s) 509, 926
HinfI GANTC 5 cut(s) 356, 382, 704, 977, 989
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 2 cut(s) 276, 1076
Hpy188I TCNGA 5 cut(s) 115, 266, 298, 795, 982
Hpy188III TCNNGA 6 cut(s) 54, 188, 386, 473, 771, 964
Hpy8I GTNNAC 2 cut(s) 276, 1076
Hpy99I CGWCG 2 cut(s) 21, 107
HpyAV CCTTC 4 cut(s) 194, 744, 967, 1028
HpyCH4III ACNGT 2 cut(s) 26, 826
HpyCH4IV ACGT 2 cut(s) 102, 811
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 836, 1057
HpyF3I CTNAG 5 cut(s) 343, 428, 645, 834, 1056
HpySE526I ACGT 2 cut(s) 102, 811
Hsp92II CATG 6 cut(s) 389, 436, 469, 562, 731, 906
HspAI GCGC 2 cut(s) 181, 606
Kzo9I GATC 3 cut(s) 56, 400, 424
LmnI GCTCC 3 cut(s) 178, 315, 562
Lsp1109I GCAGC 3 cut(s) 534, 577, 842
LweI GCATC 4 cut(s) 302, 556, 848, 915
MaeI CTAG 1 cut(s) 78
MaeII ACGT 2 cut(s) 102, 811
MaeIII GTNAC 2 cut(s) 705, 800
MalI GATC 3 cut(s) 58, 402, 426
MbiI CCGCTC 1 cut(s) 310
MboI GATC 3 cut(s) 56, 400, 424
MflI RGATCY 2 cut(s) 56, 424
MhlI GDGCHC 2 cut(s) 155, 172
MluCI AATT 6 cut(s) 334, 459, 485, 622, 1017, 1121
MlyI GAGTC 1 cut(s) 713
Mph1103I ATGCAT 3 cut(s) 549, 765, 908
MroXI GAANNNNTTC 4 cut(s) 25, 79, 381, 993
MseI TTAA 5 cut(s) 252, 332, 483, 594, 818
MslI CAYNNNNRTG 1 cut(s) 146
MspA1I CMGCKG 2 cut(s) 175, 833
MspCI CTTAAG 1 cut(s) 817
MspR9I CCNGG 2 cut(s) 61, 698
MvaI CCWGG 2 cut(s) 61, 698
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 3 cut(s) 167, 836, 1057
NdeII GATC 3 cut(s) 56, 400, 424
NlaIII CATG 6 cut(s) 389, 436, 469, 562, 731, 906
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 705
NruI TCGCGA 1 cut(s) 189
NsiI ATGCAT 3 cut(s) 549, 765, 908
NspI RCATGY 2 cut(s) 436, 469
PaeI GCATGC 1 cut(s) 436
PagI TCATGA 1 cut(s) 385
PasI CCCWGGG 1 cut(s) 697
PciI ACATGT 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PdmI GAANNNNTTC 4 cut(s) 25, 79, 381, 993
PfeI GAWTC 4 cut(s) 356, 382, 977, 989
PflMI CCANNNNNTGG 1 cut(s) 697
PfoI TCCNGGA 1 cut(s) 59
PkrI GCNGC 4 cut(s) 309, 524, 567, 832
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
PscI ACATGT 1 cut(s) 465
Psp1406I AACGTT 1 cut(s) 811
Psp6I CCWGG 2 cut(s) 59, 696
PspGI CCWGG 2 cut(s) 59, 696
PspPI GGNCC 1 cut(s) 411
PsuI RGATCY 2 cut(s) 56, 424
PvuII CAGCTG 1 cut(s) 833
RruI TCGCGA 1 cut(s) 189
RsaI GTAC 2 cut(s) 136, 823
RsaNI GTAC 2 cut(s) 135, 822
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 5 cut(s) 252, 332, 483, 594, 818
SatI GCNGC 4 cut(s) 308, 523, 566, 831
Sau3AI GATC 3 cut(s) 56, 400, 424
Sau96I GGNCC 1 cut(s) 411
ScaI AGTACT 1 cut(s) 823
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 2 cut(s) 61, 698
SduI GDGCHC 2 cut(s) 155, 172
SfaNI GCATC 4 cut(s) 302, 556, 848, 915
SfcI CTRYAG 2 cut(s) 825, 1099
SinI GGWCC 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 817
SmoI CTYRAG 1 cut(s) 817
SpeI ACTAGT 1 cut(s) 77
SphI GCATGC 1 cut(s) 436
Sse9I AATT 6 cut(s) 334, 459, 485, 622, 1017, 1121
SsiI CCGC 4 cut(s) 84, 123, 175, 308
SspI AATATT 1 cut(s) 442
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 59, 696
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 2 cut(s) 26, 826
TaiI ACGT 2 cut(s) 105, 814
TaqI TCGA 4 cut(s) 19, 403, 514, 886
TasI AATT 6 cut(s) 334, 459, 485, 622, 1017, 1121
TatI WGTACW 1 cut(s) 821
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 4 cut(s) 356, 382, 977, 989
Tru1I TTAA 5 cut(s) 252, 332, 483, 594, 818
Tru9I TTAA 5 cut(s) 252, 332, 483, 594, 818
TscAI CASTG 1 cut(s) 1030
TseFI GTSAC 1 cut(s) 705
TseI GCWGC 3 cut(s) 522, 565, 830
Tsp45I GTSAC 1 cut(s) 705
TspDTI ATGAA 8 cut(s) 374, 479, 494, 648, 662, 920, 986, 1044
TspRI CASTG 1 cut(s) 1030
Van91I CCANNNNNTGG 1 cut(s) 697
Vha464I CTTAAG 1 cut(s) 817
VpaK11BI GGWCC 1 cut(s) 411
XagI CCTNNNNNAGG 1 cut(s) 205
XapI RAATTY 2 cut(s) 485, 1017
XceI RCATGY 2 cut(s) 436, 469
XmnI GAANNNNTTC 4 cut(s) 25, 79, 381, 993
XspI CTAG 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 823
Zsp2I ATGCAT 3 cut(s) 549, 765, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.