Rw1G007720

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003610
Physical Location & Seq
Reverse (-)
16370627 .. 16374569
3943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G007720.1

Sequence Viewer

Length: 951 bp
ATGGAGAGAAGAGGCCGTCGAAACCGTTCTTCTTCTTCTCGGGGTTCAAGATCCTGGAGCAGAGGTGGAACTAGTTCCGCCGAGAAAGTGGAAAACGTCGCCGTTTCCGATGTTCCCGCCATAGTGGGTACTTGCACTTTGATGTGCCCAGAAGCAGAAAGGGCACAGCGGGAGCGCCTTCGCGATTTAGCTACCTTTGAGAGGCTGAATGGAAATCCAGCTCGCTCATCGCCATCCATTGCAGTTAAAAAGTTTTTCAGAACTATGTCCACCAAACAAGTTCAAGCATCAGATGTTCGGCCGCTCCCTGTTTTGGAGGATACTTTAAATTACCTCTTGGAGTTGTTTGATTCTAAAGACTATCCTTTTGAAGTGATTCACGACTTCCTCTTTGATCGAACAAGGTCCATAAGGCAAGATCTTAGCATGCAGAATATTGGTGGCGAGAAAGCAATTCACATGTATCAGAAAATGGTTAAATTTCATATCATCTCTCTTCATAAGCTTAGAGGTTGCAGCAGTCAAAATACTTCTCCAGTGCATCACCTCAACATGGAGCAGCTTGCAAAGACCTTGACATCTCTATTTAATCTGTATGGCGCAAATCGTCATTCCAATTCTACTAATGAAAATGAGGCTGAGTTTTGTTCTTTTTATGTGCTACTTCATCTTGGTTCTCATAGCCAACCCCTGGGGGAGTCACTTTCTTGGTGGTTTAGTCATGTGCCTTCTTCGATAATCAAGTCAAAGGAAATGCATTTTTCCCGCAAGATTTTACGATATTTTCGGATGGGTAACTATAAACGTTTCTTAAGTACTGTAGCAGCTGAGGCATCTTATCTTCAATACTGCATTATTGAGCCTTATGTTGATGAGGTAAATGACTTTCTATTATGTTATCATTACTTTTTTTGGTTTGCAATTTTGAACTCTATGGACCAACTTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

316

Amino Acids

36.48

Weight (kDa)

9.07

Isoelectric Point (pI)

57.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 48 - 294 1.6e-70 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 691
AccBSI CCGCTC 1 cut(s) 304
AccII CGCG 1 cut(s) 183
AciI CCGC 5 cut(s) 78, 117, 169, 302, 766
AclI AACGTT 1 cut(s) 805
AclWI GGATC 1 cut(s) 45
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 1 cut(s) 479
AfaI GTAC 2 cut(s) 130, 817
AfiI CCNNNNNNNGG 4 cut(s) 201, 313, 553, 691
AflII CTTAAG 1 cut(s) 811
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 5 cut(s) 48, 284, 371, 845, 928
AhlI ACTAGT 1 cut(s) 71
AjnI CCWGG 2 cut(s) 53, 690
AluBI AGCT 5 cut(s) 191, 221, 505, 562, 827
AluI AGCT 5 cut(s) 191, 221, 505, 562, 827
AlwI GGATC 1 cut(s) 45
Ama87I CYCGRG 1 cut(s) 39
AoxI GGCC 2 cut(s) 13, 299
ApeKI GCWGC 3 cut(s) 516, 559, 824
ApoI RAATTY 1 cut(s) 479
ArsI GACNNNNNNTTYG 4 cut(s) 350, 374, 382, 406
Asp700I GAANNNNTTC 3 cut(s) 25, 73, 375
AspLEI GCGC 2 cut(s) 177, 602
AspS9I GGNCC 2 cut(s) 405, 937
AsuHPI GGTGA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 39
AvaII GGWCC 2 cut(s) 405, 937
BaeGI GKGCMC 2 cut(s) 149, 166
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 3 cut(s) 528, 571, 836
BccI CCATC 2 cut(s) 241, 784
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 2 cut(s) 55, 692
BciVI GTATCC 1 cut(s) 313
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 178
BfrI CTTAAG 1 cut(s) 811
BfuI GTATCC 1 cut(s) 313
BglII AGATCT 1 cut(s) 418
BisI GCNGC 4 cut(s) 302, 517, 560, 825
BlsI GCNGC 4 cut(s) 303, 518, 561, 826
BmcAI AGTACT 1 cut(s) 817
Bme1390I CCNGG 2 cut(s) 55, 692
Bme18I GGWCC 2 cut(s) 405, 937
BmeT110I CYCGRG 1 cut(s) 39
BmgT120I GGNCC 2 cut(s) 405, 937
BmrFI CCNGG 2 cut(s) 55, 692
BmsI GCATC 3 cut(s) 296, 550, 842
BpmI CTGGAG 2 cut(s) 76, 519
Bpu10I CCTNAGC 1 cut(s) 828
BsaJI CCNNGG 2 cut(s) 690, 691
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 4 cut(s) 201, 313, 553, 691
Bse1I ACTGG 1 cut(s) 536
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 2 cut(s) 55, 692
BseDI CCNNGG 2 cut(s) 690, 691
BseGI GGATG 2 cut(s) 233, 795
BseLI CCNNNNNNNGG 4 cut(s) 201, 313, 553, 691
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 2 cut(s) 630, 819
BseNI ACTGG 1 cut(s) 536
BseSI GKGCMC 2 cut(s) 149, 166
BseX3I CGGCCG 1 cut(s) 299
BseXI GCAGC 3 cut(s) 528, 571, 836
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 302
BshFI GGCC 2 cut(s) 15, 301
BsiEI CGRYCG 1 cut(s) 302
BsiHKCI CYCGRG 1 cut(s) 39
BslI CCNNNNNNNGG 4 cut(s) 201, 313, 553, 691
BsnI GGCC 2 cut(s) 15, 301
BsoBI CYCGRG 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 149, 166
Bsp143I GATC 3 cut(s) 50, 394, 418
Bsp68I TCGCGA 1 cut(s) 183
BspACI CCGC 5 cut(s) 78, 117, 169, 302, 766
BspANI GGCC 2 cut(s) 15, 301
BspCNI CTCAG 2 cut(s) 631, 820
BspFNI CGCG 1 cut(s) 183
BspPI GGATC 1 cut(s) 45
BspTI CTTAAG 1 cut(s) 811
BsrBI CCGCTC 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 1 cut(s) 536
BssECI CCNNGG 2 cut(s) 690, 691
BssMI GATC 3 cut(s) 50, 394, 418
Bst2UI CCWGG 2 cut(s) 55, 692
Bst4CI ACNGT 2 cut(s) 26, 820
Bst6I CTCTTC 2 cut(s) 4, 501
BstAFI CTTAAG 1 cut(s) 811
BstC8I GCNNGC 3 cut(s) 223, 428, 564
BstDEI CTNAG 4 cut(s) 422, 506, 639, 828
BstENI CCTNNNNNAGG 1 cut(s) 199
BstF5I GGATG 2 cut(s) 233, 795
BstFNI CGCG 1 cut(s) 183
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 2 cut(s) 177, 602
BstKTI GATC 3 cut(s) 53, 397, 421
BstMBI GATC 3 cut(s) 50, 394, 418
BstMCI CGRYCG 1 cut(s) 302
BstMWI GCNNNNNNNGC 2 cut(s) 161, 830
BstNI CCWGG 2 cut(s) 55, 692
BstNSI RCATGY 2 cut(s) 430, 463
BstSCI CCNGG 2 cut(s) 53, 690
BstSFI CTRYAG 1 cut(s) 819
BstSLI GKGCMC 2 cut(s) 149, 166
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 528, 571, 836
BstX2I RGATCY 2 cut(s) 50, 418
BstYI RGATCY 2 cut(s) 50, 418
BstZI CGGCCG 1 cut(s) 299
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 2 cut(s) 15, 301
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 2 cut(s) 233, 795
BtsIMutI CAGTG 1 cut(s) 543
BtuMI TCGCGA 1 cut(s) 183
Cac8I GCNNGC 3 cut(s) 223, 428, 564
CfoI GCGC 2 cut(s) 177, 602
Cfr13I GGNCC 2 cut(s) 405, 937
Csp6I GTAC 2 cut(s) 129, 816
CviAII CATG 5 cut(s) 427, 460, 553, 722, 948
CviQI GTAC 2 cut(s) 129, 816
DdeI CTNAG 4 cut(s) 422, 506, 639, 828
DpnI GATC 3 cut(s) 52, 396, 420
DpnII GATC 3 cut(s) 50, 394, 418
DraI TTTAAA 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 299
EagI CGGCCG 1 cut(s) 299
Eam1104I CTCTTC 2 cut(s) 4, 501
EarI CTCTTC 2 cut(s) 4, 501
EciI GGCGGA 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 299
Eco47I GGWCC 2 cut(s) 405, 937
Eco52I CGGCCG 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 39
EcoNI CCTNNNNNAGG 1 cut(s) 199
EcoRII CCWGG 2 cut(s) 53, 690
EcoT22I ATGCAT 1 cut(s) 759
FaeI CATG 5 cut(s) 430, 463, 556, 725, 951
FatI CATG 5 cut(s) 426, 459, 552, 721, 947
FauI CCCGC 3 cut(s) 124, 162, 773
Fnu4HI GCNGC 4 cut(s) 302, 517, 560, 825
FokI GGATG 2 cut(s) 220, 802
Fsp4HI GCNGC 4 cut(s) 302, 517, 560, 825
FspBI CTAG 1 cut(s) 72
GlaI GCGC 2 cut(s) 176, 601
GluI GCNGC 4 cut(s) 302, 517, 560, 825
GsuI CTGGAG 2 cut(s) 76, 519
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 2 cut(s) 15, 301
HhaI GCGC 2 cut(s) 177, 602
Hin1II CATG 5 cut(s) 430, 463, 556, 725, 951
Hin6I GCGC 2 cut(s) 175, 600
HinP1I GCGC 2 cut(s) 175, 600
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 3 cut(s) 350, 376, 698
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 5 cut(s) 109, 260, 292, 468, 789
Hpy188III TCNNGA 3 cut(s) 48, 182, 380
Hpy8I GTNNAC 1 cut(s) 270
Hpy99I CGWCG 2 cut(s) 21, 101
HpyAV CCTTC 2 cut(s) 188, 738
HpyCH4III ACNGT 2 cut(s) 26, 820
HpyCH4IV ACGT 2 cut(s) 96, 805
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 830
HpyF3I CTNAG 4 cut(s) 422, 506, 639, 828
HpySE526I ACGT 2 cut(s) 96, 805
Hsp92II CATG 5 cut(s) 430, 463, 556, 725, 951
HspAI GCGC 2 cut(s) 175, 600
Kzo9I GATC 3 cut(s) 50, 394, 418
LmnI GCTCC 4 cut(s) 57, 172, 309, 556
LpnPI CCDG 8 cut(s) 40, 67, 162, 231, 321, 549, 677, 704
Lsp1109I GCAGC 3 cut(s) 528, 571, 836
LweI GCATC 3 cut(s) 296, 550, 842
MaeI CTAG 1 cut(s) 72
MaeII ACGT 2 cut(s) 96, 805
MaeIII GTNAC 2 cut(s) 699, 794
MalI GATC 3 cut(s) 52, 396, 420
MbiI CCGCTC 1 cut(s) 304
MboI GATC 3 cut(s) 50, 394, 418
MboII GAAGA 7 cut(s) 21, 21, 24, 27, 488, 723, 833
MflI RGATCY 2 cut(s) 50, 418
MhlI GDGCHC 2 cut(s) 149, 166
MluCI AATT 5 cut(s) 328, 453, 479, 616, 921
MlyI GAGTC 1 cut(s) 707
Mph1103I ATGCAT 1 cut(s) 759
MroXI GAANNNNTTC 3 cut(s) 25, 73, 375
MseI TTAA 5 cut(s) 246, 326, 477, 588, 812
MslI CAYNNNNRTG 1 cut(s) 140
MspA1I CMGCKG 2 cut(s) 169, 827
MspCI CTTAAG 1 cut(s) 811
MspR9I CCNGG 2 cut(s) 55, 692
MvaI CCWGG 2 cut(s) 55, 692
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 2 cut(s) 161, 830
NdeII GATC 3 cut(s) 50, 394, 418
NlaIII CATG 5 cut(s) 430, 463, 556, 725, 951
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 1 cut(s) 699
NruI TCGCGA 1 cut(s) 183
NsiI ATGCAT 1 cut(s) 759
NspI RCATGY 2 cut(s) 430, 463
PaeI GCATGC 1 cut(s) 430
PasI CCCWGGG 1 cut(s) 691
PciI ACATGT 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdmI GAANNNNTTC 3 cut(s) 25, 73, 375
PfeI GAWTC 2 cut(s) 350, 376
PflMI CCANNNNNTGG 1 cut(s) 691
PfoI TCCNGGA 1 cut(s) 53
PkrI GCNGC 4 cut(s) 303, 518, 561, 826
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
PscI ACATGT 1 cut(s) 459
Psp1406I AACGTT 1 cut(s) 805
Psp6I CCWGG 2 cut(s) 53, 690
PspGI CCWGG 2 cut(s) 53, 690
PspPI GGNCC 2 cut(s) 405, 937
PsuI RGATCY 2 cut(s) 50, 418
PvuII CAGCTG 1 cut(s) 827
RruI TCGCGA 1 cut(s) 183
RsaI GTAC 2 cut(s) 130, 817
RsaNI GTAC 2 cut(s) 129, 816
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 5 cut(s) 246, 326, 477, 588, 812
SatI GCNGC 4 cut(s) 302, 517, 560, 825
Sau3AI GATC 3 cut(s) 50, 394, 418
Sau96I GGNCC 2 cut(s) 405, 937
ScaI AGTACT 1 cut(s) 817
SchI GAGTC 1 cut(s) 707
ScrFI CCNGG 2 cut(s) 55, 692
SduI GDGCHC 2 cut(s) 149, 166
SfaNI GCATC 3 cut(s) 296, 550, 842
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 2 cut(s) 405, 937
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 811
SmoI CTYRAG 1 cut(s) 811
SpeI ACTAGT 1 cut(s) 71
SphI GCATGC 1 cut(s) 430
Sse9I AATT 5 cut(s) 328, 453, 479, 616, 921
SsiI CCGC 5 cut(s) 78, 117, 169, 302, 766
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 2 cut(s) 53, 690
TaaI ACNGT 2 cut(s) 26, 820
TaiI ACGT 2 cut(s) 99, 808
TaqI TCGA 3 cut(s) 19, 397, 734
TasI AATT 5 cut(s) 328, 453, 479, 616, 921
TatI WGTACW 1 cut(s) 815
TauI GCSGC 1 cut(s) 304
TfiI GAWTC 2 cut(s) 350, 376
Tru1I TTAA 5 cut(s) 246, 326, 477, 588, 812
Tru9I TTAA 5 cut(s) 246, 326, 477, 588, 812
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 699
TseI GCWGC 3 cut(s) 516, 559, 824
Tsp45I GTSAC 1 cut(s) 699
TspDTI ATGAA 4 cut(s) 473, 488, 642, 656
TspRI CASTG 1 cut(s) 543
Van91I CCANNNNNTGG 1 cut(s) 691
Vha464I CTTAAG 1 cut(s) 811
VpaK11BI GGWCC 2 cut(s) 405, 937
XagI CCTNNNNNAGG 1 cut(s) 199
XapI RAATTY 1 cut(s) 479
XceI RCATGY 2 cut(s) 430, 463
XmnI GAANNNNTTC 3 cut(s) 25, 73, 375
XspI CTAG 1 cut(s) 72
ZrmI AGTACT 1 cut(s) 817
Zsp2I ATGCAT 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.