Rh1DG103600

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003610
Physical Location & Seq
Forward (+)
19396143 .. 19405382
9240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG103600.1

Sequence Viewer

Length: 969 bp
ATGGAGAGAAGAGGCCGTCGAAACCGTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCGGGGTTCAAGATCCTGGAGCAGAGGTGGAACTAGTTCCGCCGAGAAAGTGGAAAACGTCGCCGTTTCCGATGTTCCCGCCATAGTGGGTACTTGCACTTTGATGTGCCCAGAAGCAGAAAGGGCACAGCGGGAGCGCCTTCGCGATTTAGCTACCTTTGAGAGGCTAAATGGAAATCCAGCTCGCTCATCGCCATCCATTGCAGTTAAAAAGTTTTTCAGAACTATGTCCACCAAACAAGTTCAAGCATCAGATGTTCGGCCGCTCCCTGTTTTGGAGGATACTTTAAATTACCTCTTAGAGTTGTTTGATTCTAAAGACTATCCTTTTGAAGTGATTCACGACTTCCTCTTTGATCGAACAAGGTCCATAAGGCAAGATCTTAGCATGCAGAATATTGGTGGCGAGAAAGCAATTCACATGTATCAGAAAATGGTTAAATTTCATATCATCTCTCTTCATAAGCTTAGAGGTTGCAGCAGTCAAAATACTTCTCCAATGCATCACCTCAACATGGAGCAGCTTGCAAAGACCTTGACATCTCTATTTAATCTGTATGGCGCAAATCGTCATTCCAATTCTACTAATGAAAATGAGGCTGAGTTTTGTTCTTTTTATGTGCTACTTCATCTTGGTTCTCATAGCCAACCCCTGGGGGAGTCACTTTCTTGGTGGTTTAGTCATGTGCCTTCTTCGATAATCAAGTCAAAGGAAATGCATTTTTCCCGAAAGATTTTACGATATTTTCGGATGGGTAACTATAAACGTTTCTTAAGTACTGTAGCAGCTGAGGCATCTTATCTTCAATACTGCATTATTGAGCCTTATGTTGATGAGGTAAATGACTTTCTATCATGTTATCATTACTTTTTTGGGTTTGCAATTTTGAACTCTATGGACCAACTTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

322

Amino Acids

36.88

Weight (kDa)

9.07

Isoelectric Point (pI)

61.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 54 - 299 2.8e-70 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 709
AccBSI CCGCTC 1 cut(s) 322
AccII CGCG 1 cut(s) 201
AciI CCGC 4 cut(s) 96, 135, 187, 320
AclI AACGTT 1 cut(s) 823
AclWI GGATC 1 cut(s) 63
AcoI YGGCCR 1 cut(s) 317
AcsI RAATTY 1 cut(s) 497
AfaI GTAC 2 cut(s) 148, 835
AfiI CCNNNNNNNGG 4 cut(s) 219, 331, 571, 709
AflII CTTAAG 1 cut(s) 829
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 5 cut(s) 66, 302, 389, 863, 946
AhlI ACTAGT 1 cut(s) 89
AjnI CCWGG 2 cut(s) 71, 708
AluBI AGCT 5 cut(s) 209, 239, 523, 580, 845
AluI AGCT 5 cut(s) 209, 239, 523, 580, 845
AlwI GGATC 1 cut(s) 63
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 2 cut(s) 13, 317
ApeKI GCWGC 3 cut(s) 534, 577, 842
ApoI RAATTY 1 cut(s) 497
ArsI GACNNNNNNTTYG 4 cut(s) 368, 392, 400, 424
Asp700I GAANNNNTTC 3 cut(s) 25, 91, 393
AspLEI GCGC 2 cut(s) 195, 620
AspS9I GGNCC 2 cut(s) 423, 955
AsuHPI GGTGA 1 cut(s) 554
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 2 cut(s) 423, 955
BaeGI GKGCMC 2 cut(s) 167, 184
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 3 cut(s) 546, 589, 854
BccI CCATC 2 cut(s) 259, 802
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 2 cut(s) 73, 710
BciVI GTATCC 1 cut(s) 331
BcuI ACTAGT 1 cut(s) 89
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 196
BfrI CTTAAG 1 cut(s) 829
BfuI GTATCC 1 cut(s) 331
BglII AGATCT 1 cut(s) 436
BisI GCNGC 4 cut(s) 320, 535, 578, 843
BlsI GCNGC 4 cut(s) 321, 536, 579, 844
BmcAI AGTACT 1 cut(s) 835
Bme1390I CCNGG 2 cut(s) 73, 710
Bme18I GGWCC 2 cut(s) 423, 955
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 2 cut(s) 423, 955
BmrFI CCNGG 2 cut(s) 73, 710
BmsI GCATC 3 cut(s) 314, 568, 860
BpmI CTGGAG 1 cut(s) 94
Bpu10I CCTNAGC 1 cut(s) 846
BsaJI CCNNGG 2 cut(s) 708, 709
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 4 cut(s) 219, 331, 571, 709
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 2 cut(s) 73, 710
BseDI CCNNGG 2 cut(s) 708, 709
BseGI GGATG 2 cut(s) 251, 813
BseLI CCNNNNNNNGG 4 cut(s) 219, 331, 571, 709
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 648, 837
BseSI GKGCMC 2 cut(s) 167, 184
BseX3I CGGCCG 1 cut(s) 317
BseXI GCAGC 3 cut(s) 546, 589, 854
Bsh1236I CGCG 1 cut(s) 201
Bsh1285I CGRYCG 1 cut(s) 320
BshFI GGCC 2 cut(s) 15, 319
BsiEI CGRYCG 1 cut(s) 320
BsiHKCI CYCGRG 1 cut(s) 57
BslI CCNNNNNNNGG 4 cut(s) 219, 331, 571, 709
BsnI GGCC 2 cut(s) 15, 319
BsoBI CYCGRG 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 167, 184
Bsp143I GATC 3 cut(s) 68, 412, 436
Bsp68I TCGCGA 1 cut(s) 201
BspACI CCGC 4 cut(s) 96, 135, 187, 320
BspANI GGCC 2 cut(s) 15, 319
BspCNI CTCAG 2 cut(s) 649, 838
BspFNI CGCG 1 cut(s) 201
BspPI GGATC 1 cut(s) 63
BspTI CTTAAG 1 cut(s) 829
BsrBI CCGCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 708, 709
BssMI GATC 3 cut(s) 68, 412, 436
Bst2UI CCWGG 2 cut(s) 73, 710
Bst4CI ACNGT 2 cut(s) 26, 838
Bst6I CTCTTC 2 cut(s) 4, 519
BstAFI CTTAAG 1 cut(s) 829
BstC8I GCNNGC 3 cut(s) 241, 446, 582
BstDEI CTNAG 5 cut(s) 355, 440, 524, 657, 846
BstENI CCTNNNNNAGG 1 cut(s) 217
BstF5I GGATG 2 cut(s) 251, 813
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 195, 620
BstKTI GATC 3 cut(s) 71, 415, 439
BstMBI GATC 3 cut(s) 68, 412, 436
BstMCI CGRYCG 1 cut(s) 320
BstMWI GCNNNNNNNGC 2 cut(s) 179, 848
BstNI CCWGG 2 cut(s) 73, 710
BstNSI RCATGY 2 cut(s) 448, 481
BstSCI CCNGG 2 cut(s) 71, 708
BstSFI CTRYAG 1 cut(s) 837
BstSLI GKGCMC 2 cut(s) 167, 184
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 3 cut(s) 546, 589, 854
BstX2I RGATCY 2 cut(s) 68, 436
BstYI RGATCY 2 cut(s) 68, 436
BstZI CGGCCG 1 cut(s) 317
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 2 cut(s) 15, 319
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 2 cut(s) 251, 813
BtuMI TCGCGA 1 cut(s) 201
Cac8I GCNNGC 3 cut(s) 241, 446, 582
CfoI GCGC 2 cut(s) 195, 620
Cfr13I GGNCC 2 cut(s) 423, 955
Csp6I GTAC 2 cut(s) 147, 834
CviAII CATG 6 cut(s) 445, 478, 571, 740, 912, 966
CviQI GTAC 2 cut(s) 147, 834
DdeI CTNAG 5 cut(s) 355, 440, 524, 657, 846
DpnI GATC 3 cut(s) 70, 414, 438
DpnII GATC 3 cut(s) 68, 412, 436
DraI TTTAAA 1 cut(s) 345
EaeI YGGCCR 1 cut(s) 317
EagI CGGCCG 1 cut(s) 317
Eam1104I CTCTTC 2 cut(s) 4, 519
EarI CTCTTC 2 cut(s) 4, 519
EciI GGCGGA 1 cut(s) 85
EclXI CGGCCG 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 423, 955
Eco52I CGGCCG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 57
EcoNI CCTNNNNNAGG 1 cut(s) 217
EcoRII CCWGG 2 cut(s) 71, 708
EcoT22I ATGCAT 2 cut(s) 561, 777
FaeI CATG 6 cut(s) 448, 481, 574, 743, 915, 969
FatI CATG 6 cut(s) 444, 477, 570, 739, 911, 965
FauI CCCGC 2 cut(s) 142, 180
Fnu4HI GCNGC 4 cut(s) 320, 535, 578, 843
FokI GGATG 2 cut(s) 238, 820
Fsp4HI GCNGC 4 cut(s) 320, 535, 578, 843
FspBI CTAG 1 cut(s) 90
GlaI GCGC 2 cut(s) 194, 619
GluI GCNGC 4 cut(s) 320, 535, 578, 843
GsuI CTGGAG 1 cut(s) 94
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 2 cut(s) 15, 319
HhaI GCGC 2 cut(s) 195, 620
Hin1II CATG 6 cut(s) 448, 481, 574, 743, 915, 969
Hin6I GCGC 2 cut(s) 193, 618
HinP1I GCGC 2 cut(s) 193, 618
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 3 cut(s) 368, 394, 716
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 1 cut(s) 288
Hpy188I TCNGA 5 cut(s) 127, 278, 310, 486, 807
Hpy188III TCNNGA 4 cut(s) 66, 200, 398, 783
Hpy8I GTNNAC 1 cut(s) 288
Hpy99I CGWCG 2 cut(s) 21, 119
HpyAV CCTTC 2 cut(s) 206, 756
HpyCH4III ACNGT 2 cut(s) 26, 838
HpyCH4IV ACGT 2 cut(s) 114, 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 848
HpyF3I CTNAG 5 cut(s) 355, 440, 524, 657, 846
HpySE526I ACGT 2 cut(s) 114, 823
Hsp92II CATG 6 cut(s) 448, 481, 574, 743, 915, 969
HspAI GCGC 2 cut(s) 193, 618
Kzo9I GATC 3 cut(s) 68, 412, 436
LmnI GCTCC 4 cut(s) 75, 190, 327, 574
LpnPI CCDG 7 cut(s) 58, 85, 180, 249, 339, 695, 722
Lsp1109I GCAGC 3 cut(s) 546, 589, 854
LweI GCATC 3 cut(s) 314, 568, 860
MaeI CTAG 1 cut(s) 90
MaeII ACGT 2 cut(s) 114, 823
MaeIII GTNAC 2 cut(s) 717, 812
MalI GATC 3 cut(s) 70, 414, 438
MbiI CCGCTC 1 cut(s) 322
MboI GATC 3 cut(s) 68, 412, 436
MflI RGATCY 2 cut(s) 68, 436
MhlI GDGCHC 2 cut(s) 167, 184
MluCI AATT 5 cut(s) 346, 471, 497, 634, 939
MlyI GAGTC 1 cut(s) 725
Mph1103I ATGCAT 2 cut(s) 561, 777
MroXI GAANNNNTTC 3 cut(s) 25, 91, 393
MseI TTAA 5 cut(s) 264, 344, 495, 606, 830
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 2 cut(s) 187, 845
MspCI CTTAAG 1 cut(s) 829
MspR9I CCNGG 2 cut(s) 73, 710
MvaI CCWGG 2 cut(s) 73, 710
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 2 cut(s) 179, 848
NdeII GATC 3 cut(s) 68, 412, 436
NlaIII CATG 6 cut(s) 448, 481, 574, 743, 915, 969
NmeAIII GCCGAG 1 cut(s) 124
NmuCI GTSAC 1 cut(s) 717
NruI TCGCGA 1 cut(s) 201
NsiI ATGCAT 2 cut(s) 561, 777
NspI RCATGY 2 cut(s) 448, 481
PaeI GCATGC 1 cut(s) 448
PasI CCCWGGG 1 cut(s) 709
PciI ACATGT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdmI GAANNNNTTC 3 cut(s) 25, 91, 393
PfeI GAWTC 2 cut(s) 368, 394
PflMI CCANNNNNTGG 1 cut(s) 709
PfoI TCCNGGA 1 cut(s) 71
PkrI GCNGC 4 cut(s) 321, 536, 579, 844
PleI GAGTC 1 cut(s) 724
PpsI GAGTC 1 cut(s) 724
PscI ACATGT 1 cut(s) 477
Psp1406I AACGTT 1 cut(s) 823
Psp6I CCWGG 2 cut(s) 71, 708
PspGI CCWGG 2 cut(s) 71, 708
PspPI GGNCC 2 cut(s) 423, 955
PsuI RGATCY 2 cut(s) 68, 436
PvuII CAGCTG 1 cut(s) 845
RruI TCGCGA 1 cut(s) 201
RsaI GTAC 2 cut(s) 148, 835
RsaNI GTAC 2 cut(s) 147, 834
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 5 cut(s) 264, 344, 495, 606, 830
SatI GCNGC 4 cut(s) 320, 535, 578, 843
Sau3AI GATC 3 cut(s) 68, 412, 436
Sau96I GGNCC 2 cut(s) 423, 955
ScaI AGTACT 1 cut(s) 835
SchI GAGTC 1 cut(s) 725
ScrFI CCNGG 2 cut(s) 73, 710
SduI GDGCHC 2 cut(s) 167, 184
SfaNI GCATC 3 cut(s) 314, 568, 860
SfcI CTRYAG 1 cut(s) 837
SinI GGWCC 2 cut(s) 423, 955
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 829
SmoI CTYRAG 1 cut(s) 829
SpeI ACTAGT 1 cut(s) 89
SphI GCATGC 1 cut(s) 448
Sse9I AATT 5 cut(s) 346, 471, 497, 634, 939
SsiI CCGC 4 cut(s) 96, 135, 187, 320
SspI AATATT 1 cut(s) 454
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 2 cut(s) 71, 708
TaaI ACNGT 2 cut(s) 26, 838
TaiI ACGT 2 cut(s) 117, 826
TaqI TCGA 3 cut(s) 19, 415, 752
TasI AATT 5 cut(s) 346, 471, 497, 634, 939
TatI WGTACW 1 cut(s) 833
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 2 cut(s) 368, 394
Tru1I TTAA 5 cut(s) 264, 344, 495, 606, 830
Tru9I TTAA 5 cut(s) 264, 344, 495, 606, 830
TseFI GTSAC 1 cut(s) 717
TseI GCWGC 3 cut(s) 534, 577, 842
Tsp45I GTSAC 1 cut(s) 717
TspDTI ATGAA 4 cut(s) 491, 506, 660, 674
Van91I CCANNNNNTGG 1 cut(s) 709
Vha464I CTTAAG 1 cut(s) 829
VpaK11BI GGWCC 2 cut(s) 423, 955
XagI CCTNNNNNAGG 1 cut(s) 217
XapI RAATTY 1 cut(s) 497
XceI RCATGY 2 cut(s) 448, 481
XmnI GAANNNNTTC 3 cut(s) 25, 91, 393
XspI CTAG 1 cut(s) 90
ZrmI AGTACT 1 cut(s) 835
Zsp2I ATGCAT 2 cut(s) 561, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.