FvH4_7g04370

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003610
Physical Location & Seq
Reverse (-)
4948512 .. 4955551
7040 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04370.t7

Sequence Viewer

Length: 1257 bp
ATGGAGAGGAGAGGGCGTCAAAGAAACCATTCTTCTTCTTCTTCTTCTTCTTCTAGGGGCTCAAGATCAACCACCGGCAAATCCTGGCGCAGAGGTGGAACTAGCTCCGCCGATTCAGTCCAAAACGCCACCGTTTCTGATGCTCTCCCCATCGCAGGCACCTGCACTTTGATGTGCCCAGAAGCTGAGAGAGCACAACGTGAAAGGCTTCGCGATTTGGCACCCTTTGAGAGGCTTAATGGAAACCCAGCTCAGTCTTCACCCTCCATTGCCGTTAAAAAGTTTTTCAGAACTATGTCTTCCAAACAAGTTGAACCGTCTGATGTTCGGCCGCTTCCTGTCTTGGAGGATACTTTAGATTACCTCTTGAAGCTGTTTGATTCGAGAGACTATCCGTTTGAAGTGGTTCACGACTTCTTGTTTGATCGGACCAGGTCCATAAGGCAGGATCTTAGCATGCAGAATATTGTCGGGGAGAAAGCAATTCAAATGTACCAGAAAATGGTTAAATTTCATATCATTTCTCTTCATAATCTTCGAGGTTGCAGCAGTCAAAATACTTCTCCAATGCATCACCTCAACATGGAGCAGCTTGCAAAGACCTTAACATCTCTATTTAATCTGTATGATGCAAACCGTCATTCCAATTCTACTTACGAAAACGAGGCTGAGTTTTGTTCTTTTTATGTGCTACTTCATCTTGGTTCTCATAGCCAACCCATGGGGGAGTCACTTTCTTGGTGGTTTAGTCATGTGCCTTCTTCATTAATCAAGTCAAAGGAAATGCAGTTTTCCCGAAAGATTTTAAGATATTTCCGGATGGGTAACTATAGACGTTTCTTAAGTACTGTAGCAGCCGAGGCATCTTATCTTCAATATTGCATCATCGAGCCTTATGTTGATGAGGTTCGAGTTTTAGCTGTGTCCTGCATCAATAGTTGCGGGTACAAGCTTCATCCATATCCTCTGTCACATTTATCCAAACTCCTGATTATGAAGGAATCAGATTTGGTATCTTTTCTCAAATCCTGTGGTCTTCAAATTTGCACGGATGAAGATGGACATAATTTATTGCCCTCTAAGCAAACGACCTTTTGTCATCCCAAGGATGGGTCTCAAAACTACAGGTTTTGGGGTATGGAACAATTTGAGAGTGGGTGCATGAGCCCTCTCTTGCCCATAGATGGGTCTGCCAGTGTCATAGAGCACCTAGCCAGGTGGCCAGTACCAGTTTGGGGTTGGAGTCATGCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

419

Amino Acids

47.61

Weight (kDa)

8.68

Isoelectric Point (pI)

61.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 58 - 305 2.1e-72 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 170
AccB1I GGYRCC 2 cut(s) 158, 220
AccB7I CCANNNNNTGG 2 cut(s) 502, 721
AccII CGCG 1 cut(s) 213
AccIII TCCGGA 1 cut(s) 816
AciI CCGC 3 cut(s) 108, 332, 942
AclWI GGATC 1 cut(s) 456
AcoI YGGCCR 2 cut(s) 329, 1220
AcsI RAATTY 2 cut(s) 509, 1041
AcyI GRCGYC 1 cut(s) 16
AdeI CACNNNGTG 1 cut(s) 200
AfaI GTAC 4 cut(s) 494, 847, 947, 1227
AflII CTTAAG 1 cut(s) 841
AgsI TTSAA 6 cut(s) 314, 370, 401, 488, 875, 1040
AhdI GACNNNNNGTC 1 cut(s) 1095
AjnI CCWGG 3 cut(s) 83, 431, 1214
AluBI AGCT 7 cut(s) 105, 185, 251, 373, 592, 920, 952
AluI AGCT 7 cut(s) 105, 185, 251, 373, 592, 920, 952
Alw21I GWGCWC 2 cut(s) 196, 1209
Alw26I GTCTC 2 cut(s) 381, 1119
AlwI GGATC 1 cut(s) 456
AlwNI CAGNNNCTG 1 cut(s) 185
Aor13HI TCCGGA 1 cut(s) 816
AoxI GGCC 2 cut(s) 329, 1220
ApeKI GCWGC 3 cut(s) 546, 589, 854
ApoI RAATTY 2 cut(s) 509, 1041
ArsI GACNNNNNNTTYG 4 cut(s) 380, 404, 412, 436
AseI ATTAAT 1 cut(s) 767
Asp700I GAANNNNTTC 3 cut(s) 28, 207, 405
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 2 cut(s) 429, 435
AsuHPI GGTGA 2 cut(s) 252, 566
AvaII GGWCC 2 cut(s) 429, 435
BaeGI GKGCMC 1 cut(s) 179
BalI TGGCCA 1 cut(s) 1222
BanI GGYRCC 2 cut(s) 158, 220
BanII GRGCYC 2 cut(s) 62, 1169
BbsI GAAGAC 3 cut(s) 249, 291, 1028
Bbv12I GWGCWC 2 cut(s) 196, 1209
BbvI GCAGC 3 cut(s) 558, 601, 866
BccI CCATC 5 cut(s) 158, 814, 1052, 1103, 1178
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 3 cut(s) 85, 433, 1216
BciVI GTATCC 1 cut(s) 343
BcoDI GTCTC 2 cut(s) 381, 1119
BfaI CTAG 4 cut(s) 54, 102, 1211, 1255
BfmI CTRYAG 3 cut(s) 829, 849, 1123
BfrI CTTAAG 1 cut(s) 841
BfuAI ACCTGC 1 cut(s) 170
BfuI GTATCC 1 cut(s) 343
BisI GCNGC 4 cut(s) 332, 547, 590, 855
BlsI GCNGC 4 cut(s) 333, 548, 591, 856
BmcAI AGTACT 1 cut(s) 847
Bme1390I CCNGG 3 cut(s) 85, 433, 1216
Bme18I GGWCC 2 cut(s) 429, 435
BmeRI GACNNNNNGTC 1 cut(s) 1095
BmgT120I GGNCC 2 cut(s) 429, 435
BmiI GGNNCC 2 cut(s) 160, 222
BmrFI CCNGG 3 cut(s) 85, 433, 1216
BmsI GCATC 6 cut(s) 130, 580, 619, 872, 891, 939
BpiI GAAGAC 3 cut(s) 249, 291, 1028
BpuEI CTTGAG 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 1119
BsaJI CCNNGG 3 cut(s) 720, 858, 1104
BsaWI WCCGGW 1 cut(s) 816
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 1194, 1223, 1229
Bse3DI GCAATG 1 cut(s) 267
BseAI TCCGGA 1 cut(s) 816
BseBI CCWGG 3 cut(s) 85, 433, 1216
BseDI CCNNGG 3 cut(s) 720, 858, 1104
BseGI GGATG 5 cut(s) 825, 955, 1057, 1099, 1114
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 3 cut(s) 177, 266, 660
BseNI ACTGG 3 cut(s) 1194, 1223, 1229
BseRI GAGGAG 1 cut(s) 22
BseSI GKGCMC 1 cut(s) 179
BseX3I CGGCCG 1 cut(s) 329
BseXI GCAGC 3 cut(s) 558, 601, 866
BseYI CCCAGC 1 cut(s) 247
BsgI GTGCAG 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 332
BshFI GGCC 2 cut(s) 331, 1222
BshNI GGYRCC 2 cut(s) 158, 220
BsiEI CGRYCG 1 cut(s) 332
BsiHKAI GWGCWC 2 cut(s) 196, 1209
BsiSI CCGG 2 cut(s) 75, 817
BsmAI GTCTC 2 cut(s) 381, 1119
BsnI GGCC 2 cut(s) 331, 1222
Bso31I GGTCTC 1 cut(s) 1119
Bsp1286I GDGCHC 5 cut(s) 62, 179, 196, 1169, 1209
Bsp13I TCCGGA 1 cut(s) 816
Bsp143I GATC 3 cut(s) 65, 424, 448
Bsp19I CCATGG 1 cut(s) 720
Bsp68I TCGCGA 1 cut(s) 213
BspACI CCGC 3 cut(s) 108, 332, 942
BspANI GGCC 2 cut(s) 331, 1222
BspCNI CTCAG 3 cut(s) 178, 265, 661
BspEI TCCGGA 1 cut(s) 816
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 2 cut(s) 160, 222
BspMI ACCTGC 1 cut(s) 170
BspPI GGATC 1 cut(s) 456
BspT107I GGYRCC 2 cut(s) 158, 220
BspTI CTTAAG 1 cut(s) 841
BspTNI GGTCTC 1 cut(s) 1119
BsrDI GCAATG 1 cut(s) 267
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 3 cut(s) 1194, 1223, 1229
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 720, 858, 1104
BssMI GATC 3 cut(s) 65, 424, 448
BssNI GRCGYC 1 cut(s) 16
BssT1I CCWWGG 2 cut(s) 720, 1104
Bst2UI CCWGG 3 cut(s) 85, 433, 1216
Bst4CI ACNGT 4 cut(s) 133, 318, 638, 850
Bst6I CTCTTC 1 cut(s) 531
BstACI GRCGYC 1 cut(s) 16
BstAFI CTTAAG 1 cut(s) 841
BstC8I GCNNGC 3 cut(s) 157, 458, 594
BstDEI CTNAG 5 cut(s) 186, 252, 452, 669, 1080
BstDSI CCRYGG 1 cut(s) 720
BstENI CCTNNNNNAGG 1 cut(s) 229
BstF5I GGATG 5 cut(s) 825, 955, 1057, 1099, 1114
BstFNI CGCG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 3 cut(s) 68, 427, 451
BstMAI GTCTC 2 cut(s) 381, 1119
BstMBI GATC 3 cut(s) 65, 424, 448
BstMCI CGRYCG 1 cut(s) 332
BstMWI GCNNNNNNNGC 3 cut(s) 191, 860, 1081
BstNI CCWGG 3 cut(s) 85, 433, 1216
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 3 cut(s) 83, 431, 1214
BstSFI CTRYAG 3 cut(s) 829, 849, 1123
BstSLI GKGCMC 1 cut(s) 179
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 3 cut(s) 558, 601, 866
BstV2I GAAGAC 3 cut(s) 249, 291, 1028
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BstZI CGGCCG 1 cut(s) 329
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 2 cut(s) 331, 1222
BtgI CCRYGG 1 cut(s) 720
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 5 cut(s) 825, 955, 1057, 1099, 1114
BtsIMutI CAGTG 1 cut(s) 1201
BtuMI TCGCGA 1 cut(s) 213
BveI ACCTGC 1 cut(s) 170
Cac8I GCNNGC 3 cut(s) 157, 458, 594
CaiI CAGNNNCTG 1 cut(s) 185
CfoI GCGC 1 cut(s) 90
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 429, 435
CseI GACGC 1 cut(s) 5
CsiI ACCWGGT 1 cut(s) 431
Csp6I GTAC 4 cut(s) 493, 846, 946, 1226
CspCI CAANNNNNGTGG 2 cut(s) 1012, 1047
CviAII CATG 6 cut(s) 457, 583, 721, 752, 1162, 1247
CviQI GTAC 4 cut(s) 493, 846, 946, 1226
DdeI CTNAG 5 cut(s) 186, 252, 452, 669, 1080
DpnI GATC 3 cut(s) 67, 426, 450
DpnII GATC 3 cut(s) 65, 424, 448
DraIII CACNNNGTG 1 cut(s) 200
DriI GACNNNNNGTC 1 cut(s) 1095
EaeI YGGCCR 2 cut(s) 329, 1220
EagI CGGCCG 1 cut(s) 329
Eam1104I CTCTTC 1 cut(s) 531
Eam1105I GACNNNNNGTC 1 cut(s) 1095
EarI CTCTTC 1 cut(s) 531
EciI GGCGGA 1 cut(s) 97
EclXI CGGCCG 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 720, 1104
Eco24I GRGCYC 2 cut(s) 62, 1169
Eco31I GGTCTC 1 cut(s) 1119
Eco47I GGWCC 2 cut(s) 429, 435
Eco52I CGGCCG 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 229
EcoRII CCWGG 3 cut(s) 83, 431, 1214
EcoT14I CCWWGG 2 cut(s) 720, 1104
EcoT22I ATGCAT 1 cut(s) 573
EcoT38I GRGCYC 2 cut(s) 62, 1169
ErhI CCWWGG 2 cut(s) 720, 1104
FaeI CATG 6 cut(s) 460, 586, 724, 755, 1165, 1250
FatI CATG 6 cut(s) 456, 582, 720, 751, 1161, 1246
FauI CCCGC 1 cut(s) 935
Fnu4HI GCNGC 4 cut(s) 332, 547, 590, 855
FokI GGATG 5 cut(s) 832, 942, 1064, 1086, 1121
FriOI GRGCYC 2 cut(s) 62, 1169
Fsp4HI GCNGC 4 cut(s) 332, 547, 590, 855
FspBI CTAG 4 cut(s) 54, 102, 1211, 1255
GlaI GCGC 1 cut(s) 89
GluI GCNGC 4 cut(s) 332, 547, 590, 855
GsaI CCCAGC 1 cut(s) 251
HaeIII GGCC 2 cut(s) 331, 1222
HapII CCGG 2 cut(s) 75, 817
HgaI GACGC 1 cut(s) 5
HhaI GCGC 1 cut(s) 90
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 6 cut(s) 460, 586, 724, 755, 1165, 1250
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 950
HinfI GANTC 5 cut(s) 113, 380, 728, 1001, 1243
HpaII CCGG 2 cut(s) 75, 817
HphI GGTGA 2 cut(s) 252, 566
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 5 cut(s) 139, 290, 322, 429, 1006
Hpy188III TCNNGA 8 cut(s) 63, 212, 367, 384, 410, 795, 817, 988
Hpy8I GTNNAC 1 cut(s) 409
HpyAV CCTTC 2 cut(s) 768, 991
HpyCH4III ACNGT 4 cut(s) 133, 318, 638, 850
HpyCH4IV ACGT 2 cut(s) 199, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 860, 1081
HpyF3I CTNAG 5 cut(s) 186, 252, 452, 669, 1080
HpySE526I ACGT 2 cut(s) 199, 835
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 6 cut(s) 460, 586, 724, 755, 1165, 1250
HspAI GCGC 1 cut(s) 88
Kpn2I TCCGGA 1 cut(s) 816
Kzo9I GATC 3 cut(s) 65, 424, 448
LmnI GCTCC 2 cut(s) 110, 586
Lsp1109I GCAGC 3 cut(s) 558, 601, 866
LweI GCATC 6 cut(s) 130, 580, 619, 872, 891, 939
MabI ACCWGGT 1 cut(s) 431
MaeI CTAG 4 cut(s) 54, 102, 1211, 1255
MaeII ACGT 2 cut(s) 199, 835
MaeIII GTNAC 3 cut(s) 729, 824, 969
MalI GATC 3 cut(s) 67, 426, 450
MboI GATC 3 cut(s) 65, 424, 448
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 5 cut(s) 62, 179, 196, 1169, 1209
MlsI TGGCCA 1 cut(s) 1222
MluCI AATT 6 cut(s) 483, 509, 646, 1041, 1066, 1145
MluNI TGGCCA 1 cut(s) 1222
MlyI GAGTC 2 cut(s) 737, 1252
MmeI TCCRAC 1 cut(s) 1220
Mox20I TGGCCA 1 cut(s) 1222
Mph1103I ATGCAT 1 cut(s) 573
MroI TCCGGA 1 cut(s) 816
MroXI GAANNNNTTC 3 cut(s) 28, 207, 405
MscI TGGCCA 1 cut(s) 1222
MseI TTAA 8 cut(s) 237, 276, 507, 605, 618, 767, 806, 842
MslI CAYNNNNRTG 1 cut(s) 170
Msp20I TGGCCA 1 cut(s) 1222
MspCI CTTAAG 1 cut(s) 841
MspI CCGG 2 cut(s) 75, 817
MspR9I CCNGG 3 cut(s) 85, 433, 1216
MvaI CCWGG 3 cut(s) 85, 433, 1216
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 3 cut(s) 191, 860, 1081
NcoI CCATGG 1 cut(s) 720
NdeII GATC 3 cut(s) 65, 424, 448
NlaIII CATG 6 cut(s) 460, 586, 724, 755, 1165, 1250
NlaIV GGNNCC 2 cut(s) 160, 222
NmeAIII GCCGAG 1 cut(s) 883
NmuCI GTSAC 2 cut(s) 729, 969
NruI TCGCGA 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 573
NspI RCATGY 1 cut(s) 460
PaeI GCATGC 1 cut(s) 460
PaqCI CACCTGC 1 cut(s) 170
PdmI GAANNNNTTC 3 cut(s) 28, 207, 405
PfeI GAWTC 3 cut(s) 113, 380, 1001
PflFI GACNNNGTC 1 cut(s) 433
PflMI CCANNNNNTGG 2 cut(s) 502, 721
PkrI GCNGC 4 cut(s) 333, 548, 591, 856
PleI GAGTC 2 cut(s) 736, 1251
PpsI GAGTC 2 cut(s) 736, 1251
PshBI ATTAAT 1 cut(s) 767
Psp6I CCWGG 3 cut(s) 83, 431, 1214
PspFI CCCAGC 1 cut(s) 247
PspGI CCWGG 3 cut(s) 83, 431, 1214
PspN4I GGNNCC 2 cut(s) 160, 222
PspPI GGNCC 2 cut(s) 429, 435
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 448
PsyI GACNNNGTC 1 cut(s) 433
RruI TCGCGA 1 cut(s) 213
RsaI GTAC 4 cut(s) 494, 847, 947, 1227
RsaNI GTAC 4 cut(s) 493, 846, 946, 1226
RseI CAYNNNNRTG 1 cut(s) 170
SaqAI TTAA 8 cut(s) 237, 276, 507, 605, 618, 767, 806, 842
SatI GCNGC 4 cut(s) 332, 547, 590, 855
Sau3AI GATC 3 cut(s) 65, 424, 448
Sau96I GGNCC 2 cut(s) 429, 435
ScaI AGTACT 1 cut(s) 847
SchI GAGTC 2 cut(s) 737, 1252
ScrFI CCNGG 3 cut(s) 85, 433, 1216
SduI GDGCHC 5 cut(s) 62, 179, 196, 1169, 1209
SexAI ACCWGGT 1 cut(s) 431
SfaNI GCATC 6 cut(s) 130, 580, 619, 872, 891, 939
SfcI CTRYAG 3 cut(s) 829, 849, 1123
SinI GGWCC 2 cut(s) 429, 435
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 2 cut(s) 61, 841
SmoI CTYRAG 2 cut(s) 61, 841
SphI GCATGC 1 cut(s) 460
Sse9I AATT 6 cut(s) 483, 509, 646, 1041, 1066, 1145
SsiI CCGC 3 cut(s) 108, 332, 942
SspI AATATT 2 cut(s) 466, 878
SspMI CTAG 4 cut(s) 54, 102, 1211, 1255
StyD4I CCNGG 3 cut(s) 83, 431, 1214
StyI CCWWGG 2 cut(s) 720, 1104
TaaI ACNGT 4 cut(s) 133, 318, 638, 850
TaiI ACGT 2 cut(s) 202, 838
TaqI TCGA 4 cut(s) 383, 538, 888, 910
TasI AATT 6 cut(s) 483, 509, 646, 1041, 1066, 1145
TatI WGTACW 1 cut(s) 845
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 3 cut(s) 113, 380, 1001
Tru1I TTAA 8 cut(s) 237, 276, 507, 605, 618, 767, 806, 842
Tru9I TTAA 8 cut(s) 237, 276, 507, 605, 618, 767, 806, 842
TscAI CASTG 1 cut(s) 1201
TseFI GTSAC 2 cut(s) 729, 969
TseI GCWGC 3 cut(s) 546, 589, 854
Tsp45I GTSAC 2 cut(s) 729, 969
TspDTI ATGAA 7 cut(s) 503, 518, 686, 753, 944, 1010, 1068
TspGWI ACGGA 2 cut(s) 384, 1064
TspRI CASTG 1 cut(s) 1201
Tth111I GACNNNGTC 1 cut(s) 433
Van91I CCANNNNNTGG 2 cut(s) 502, 721
Vha464I CTTAAG 1 cut(s) 841
VpaK11BI GGWCC 2 cut(s) 429, 435
VspI ATTAAT 1 cut(s) 767
XagI CCTNNNNNAGG 1 cut(s) 229
XapI RAATTY 2 cut(s) 509, 1041
XceI RCATGY 1 cut(s) 460
XcmI CCANNNNNNNNNTGG 2 cut(s) 1230, 1236
XmnI GAANNNNTTC 3 cut(s) 28, 207, 405
XspI CTAG 4 cut(s) 54, 102, 1211, 1255
ZrmI AGTACT 1 cut(s) 847
Zsp2I ATGCAT 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.