MD02G1270900.v1.1

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003610
Physical Location & Seq
Reverse (-)
32560206 .. 32564701
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1270900.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGAGAGGCGAGGGCGTGGTCGAAACGTTTCTTCTTCCCAATCCACGCGTGGCCGCTCGAACAGGTTCGTTTCGAAACCCACCTCCGCCGCAACTCCCAAATCCTCGAGCGGAGGCGCAACTGGTACTGAGAAAGCACAAAAAGACGCCGTAGATAACCATTCCGACGTTCCCGCCATAGTGGGCACTTGCCCTTTGATGTGCCCGGAAGGAGAGAGGGCGCAGCGTGAGCGGCTTCGTGATTTAGCAGTGTTTGAGAGGCTCCATGGAAATCCAAGGCAATCATCTCCGGACCTCGCTGTTAAAAAATTTTTCAGAACTACGTCTAACAAACAAGTTGAAGCATCGGATGTTCGGCCGCTCCCTGTGTTGGAAGACACCTTAAATTACCTTTTAAACTTGTTTGATTCTAGAGAATATCCTTTTGAAGTGGTTCATGACTTCCTCTTTGATCGAACAAGGTCGATAAGACAGGATCTTAGCATGCAGAATGTTGTCAACGATAAGGTCATCCACATGTATGAGAAAATGGTAAAATTTCATATCATATCCCTACGCAAGCTTCGAGGTTGTCGCAGTCAAAATACTTCTTCAACCCATTACCTCAACATGGAACAGCTTGCAAAGACTTTGACATCTCTATTTGATCTGTATGAAGCACATAGGCATTCCAATTCTACATATGAAAACGAAGCTGAGTTTTGTTCGTTTTATGTGCTGCTTCATCTTGGTCCTAACAGCCAACCGATGGGAGAGTCCCTTTCATGGTGGTTTTCTCACTTGCCTTCTTTGTTAATCAAATCGAAGGAAATGTGCTTTTCCCGAAAGATTTTACGATTTTTCCGGATGGGCAACTATAAGCGTTTCTTAAGCACTATAGCAGCTGAGGCATCTTATCTTCAATACTGCATTCTCGAGCCTTATATCAATCAGGTTCGAGCGCTAGCTGTCTTGTGTATAAATAATGGTGGATACAGGCTTCATCCGTATCCGCTGGCACACTTGTCTAAGCTCTTGATGATGACGGAATCAGATTTGGAGTCTCTTTGTAGAGCTTGTGGTCTTGATATTTGCACAGATGAAGATGGATGTAATTTGTTACCCACTAAGCAAACCACCTTCTGTCATCCAAAGGATGGATTTCAAAGCTACAGAGTTTTGGGTCTGGAAGAATTCGAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

395

Amino Acids

45.11

Weight (kDa)

8.74

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 67 - 357 7.1e-81 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 748, 1136
AccBSI CCGCTC 4 cut(s) 57, 111, 232, 361
AccII CGCG 1 cut(s) 49
AccIII TCCGGA 2 cut(s) 289, 843
AciI CCGC 8 cut(s) 55, 87, 90, 111, 174, 232, 359, 992
AclI AACGTT 1 cut(s) 27
AclWI GGATC 1 cut(s) 483
AcoI YGGCCR 2 cut(s) 52, 356
AcsI RAATTY 3 cut(s) 308, 536, 1172
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 127
AfeI AGCGCT 1 cut(s) 942
AfiI CCNNNNNNNGG 5 cut(s) 370, 610, 748, 765, 1136
AflII CTTAAG 1 cut(s) 868
AflIII ACRYGT 2 cut(s) 47, 516
AgsI TTSAA 5 cut(s) 341, 428, 594, 902, 1145
AluBI AGCT 8 cut(s) 562, 619, 695, 884, 947, 1012, 1055, 1149
AluI AGCT 8 cut(s) 562, 619, 695, 884, 947, 1012, 1055, 1149
Alw26I GTCTC 1 cut(s) 1047
AlwI GGATC 1 cut(s) 483
Ama87I CYCGRG 2 cut(s) 106, 914
Aor13HI TCCGGA 2 cut(s) 289, 843
Aor51HI AGCGCT 1 cut(s) 942
AoxI GGCC 2 cut(s) 52, 356
ApeKI GCWGC 3 cut(s) 223, 718, 881
ApoI RAATTY 3 cut(s) 308, 536, 1172
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
Asp700I GAANNNNTTC 3 cut(s) 28, 65, 432
AspLEI GCGC 3 cut(s) 119, 223, 943
AspS9I GGNCC 2 cut(s) 292, 731
AsuC2I CCSGG 1 cut(s) 206
AsuII TTCGAA 1 cut(s) 74
AsuNHI GCTAGC 1 cut(s) 943
AvaI CYCGRG 2 cut(s) 106, 914
AvaII GGWCC 2 cut(s) 292, 731
BaeGI GKGCMC 2 cut(s) 188, 206
BbsI GAAGAC 1 cut(s) 381
BbvCI CCTCAGC 1 cut(s) 885
BbvI GCAGC 3 cut(s) 235, 705, 893
BccI CCATC 4 cut(s) 742, 841, 1079, 1130
BceAI ACGGC 1 cut(s) 134
BciVI GTATCC 2 cut(s) 965, 999
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 1047
BfaI CTAG 2 cut(s) 411, 944
BfmI CTRYAG 2 cut(s) 876, 1150
BfoI RGCGCY 1 cut(s) 944
BfrI CTTAAG 1 cut(s) 868
BfuI GTATCC 2 cut(s) 965, 999
BisI GCNGC 7 cut(s) 55, 90, 224, 233, 359, 719, 882
BlsI GCNGC 7 cut(s) 56, 91, 225, 234, 360, 720, 883
Bme1390I CCNGG 1 cut(s) 206
Bme18I GGWCC 2 cut(s) 292, 731
BmeT110I CYCGRG 2 cut(s) 106, 914
BmgT120I GGNCC 2 cut(s) 292, 731
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 2 cut(s) 353, 899
BmtI GCTAGC 1 cut(s) 947
BpiI GAAGAC 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 885
Bpu14I TTCGAA 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 265, 275
BsaWI WCCGGW 2 cut(s) 289, 843
Bsc4I CCNNNNNNNGG 5 cut(s) 370, 610, 748, 765, 1136
Bse1I ACTGG 1 cut(s) 127
BseAI TCCGGA 2 cut(s) 289, 843
BseDI CCNNGG 2 cut(s) 265, 275
BseGI GGATG 7 cut(s) 355, 510, 852, 982, 1094, 1126, 1141
BseLI CCNNNNNNNGG 5 cut(s) 370, 610, 748, 765, 1136
BseMII CTCAG 3 cut(s) 120, 687, 876
BseNI ACTGG 1 cut(s) 127
BseSI GKGCMC 2 cut(s) 188, 206
BseX3I CGGCCG 1 cut(s) 356
BseXI GCAGC 3 cut(s) 235, 705, 893
Bsh1236I CGCG 1 cut(s) 49
Bsh1285I CGRYCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 54, 358
BsiEI CGRYCG 1 cut(s) 359
BsiHKCI CYCGRG 2 cut(s) 106, 914
BsiSI CCGG 3 cut(s) 206, 290, 844
BslFI GGGAC 1 cut(s) 742
BslI CCNNNNNNNGG 5 cut(s) 370, 610, 748, 765, 1136
BsmAI GTCTC 1 cut(s) 1047
BsmFI GGGAC 1 cut(s) 742
BsmI GAATGC 2 cut(s) 667, 909
BsnI GGCC 2 cut(s) 54, 358
BsoBI CYCGRG 2 cut(s) 106, 914
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 188, 206
Bsp13I TCCGGA 2 cut(s) 289, 843
Bsp143I GATC 3 cut(s) 451, 475, 646
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 8 cut(s) 55, 87, 90, 111, 174, 232, 359, 992
BspANI GGCC 2 cut(s) 54, 358
BspCNI CTCAG 3 cut(s) 121, 688, 877
BspEI TCCGGA 2 cut(s) 289, 843
BspFNI CGCG 1 cut(s) 49
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 1 cut(s) 263
BspOI GCTAGC 1 cut(s) 947
BspPI GGATC 1 cut(s) 483
BspT104I TTCGAA 1 cut(s) 74
BspTI CTTAAG 1 cut(s) 868
BsrBI CCGCTC 4 cut(s) 57, 111, 232, 361
BsrI ACTGG 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 265, 275
BssMI GATC 3 cut(s) 451, 475, 646
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 265, 275
BstACI GRCGYC 1 cut(s) 147
BstAFI CTTAAG 1 cut(s) 868
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 5 cut(s) 485, 560, 621, 945, 996
BstDEI CTNAG 6 cut(s) 129, 479, 696, 885, 1008, 1107
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 7 cut(s) 355, 510, 852, 982, 1094, 1126, 1141
BstFNI CGCG 1 cut(s) 49
BstH2I RGCGCY 1 cut(s) 944
BstHHI GCGC 3 cut(s) 119, 223, 943
BstKTI GATC 3 cut(s) 454, 478, 649
BstMAI GTCTC 1 cut(s) 1047
BstMBI GATC 3 cut(s) 451, 475, 646
BstMCI CGRYCG 1 cut(s) 359
BstMWI GCNNNNNNNGC 3 cut(s) 229, 232, 887
BstNSI RCATGY 2 cut(s) 487, 520
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 2 cut(s) 876, 1150
BstSLI GKGCMC 2 cut(s) 188, 206
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 3 cut(s) 235, 705, 893
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
BstZI CGGCCG 1 cut(s) 356
BsuI GTATCC 2 cut(s) 965, 999
BsuRI GGCC 2 cut(s) 54, 358
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 7 cut(s) 355, 510, 852, 982, 1094, 1126, 1141
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 5 cut(s) 485, 560, 621, 945, 996
CciI TCATGA 1 cut(s) 436
CfoI GCGC 3 cut(s) 119, 223, 943
Cfr13I GGNCC 2 cut(s) 292, 731
CseI GACGC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 126
CviAII CATG 6 cut(s) 266, 437, 484, 517, 610, 765
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 6 cut(s) 129, 479, 696, 885, 1008, 1107
DpnI GATC 3 cut(s) 453, 477, 648
DpnII GATC 3 cut(s) 451, 475, 646
DraI TTTAAA 1 cut(s) 396
EaeI YGGCCR 2 cut(s) 52, 356
EagI CGGCCG 1 cut(s) 356
EciI GGCGGA 1 cut(s) 76
EclXI CGGCCG 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 265, 275
Eco47I GGWCC 2 cut(s) 292, 731
Eco47III AGCGCT 1 cut(s) 942
Eco52I CGGCCG 1 cut(s) 356
Eco88I CYCGRG 2 cut(s) 106, 914
EcoRI GAATTC 1 cut(s) 1172
EcoT14I CCWWGG 2 cut(s) 265, 275
ErhI CCWWGG 2 cut(s) 265, 275
FaeI CATG 6 cut(s) 269, 440, 487, 520, 613, 768
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FaqI GGGAC 1 cut(s) 742
FatI CATG 6 cut(s) 265, 436, 483, 516, 609, 764
FauI CCCGC 1 cut(s) 181
FauNDI CATATG 1 cut(s) 682
Fnu4HI GCNGC 7 cut(s) 55, 90, 224, 233, 359, 719, 882
FokI GGATG 7 cut(s) 362, 497, 859, 969, 1101, 1113, 1148
Fsp4HI GCNGC 7 cut(s) 55, 90, 224, 233, 359, 719, 882
FspBI CTAG 2 cut(s) 411, 944
GlaI GCGC 3 cut(s) 118, 222, 942
GluI GCNGC 7 cut(s) 55, 90, 224, 233, 359, 719, 882
HaeII RGCGCY 1 cut(s) 944
HaeIII GGCC 2 cut(s) 54, 358
HapII CCGG 3 cut(s) 206, 290, 844
HgaI GACGC 1 cut(s) 155
HhaI GCGC 3 cut(s) 119, 223, 943
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 6 cut(s) 269, 440, 487, 520, 613, 768
Hin6I GCGC 3 cut(s) 117, 221, 941
HinP1I GCGC 3 cut(s) 117, 221, 941
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 4 cut(s) 407, 755, 1028, 1040
HpaII CCGG 3 cut(s) 206, 290, 844
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 4 cut(s) 166, 317, 349, 1033
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 4 cut(s) 203, 795, 799, 1129
HpyCH4IV ACGT 3 cut(s) 27, 168, 323
HpyCH4V TGCA 4 cut(s) 487, 623, 909, 1074
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 232, 887
HpyF3I CTNAG 6 cut(s) 129, 479, 696, 885, 1008, 1107
HpySE526I ACGT 3 cut(s) 27, 168, 323
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 6 cut(s) 269, 440, 487, 520, 613, 768
HspAI GCGC 3 cut(s) 117, 221, 941
Kpn2I TCCGGA 2 cut(s) 289, 843
Kzo9I GATC 3 cut(s) 451, 475, 646
LmnI GCTCC 2 cut(s) 267, 366
Lsp1109I GCAGC 3 cut(s) 235, 705, 893
LweI GCATC 2 cut(s) 353, 899
MaeI CTAG 2 cut(s) 411, 944
MaeII ACGT 3 cut(s) 27, 168, 323
MaeIII GTNAC 1 cut(s) 1098
MalI GATC 3 cut(s) 453, 477, 648
MbiI CCGCTC 4 cut(s) 57, 111, 232, 361
MboI GATC 3 cut(s) 451, 475, 646
MboII GAAGA 7 cut(s) 24, 27, 386, 582, 890, 1094, 1181
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 2 cut(s) 188, 206
MluCI AATT 6 cut(s) 308, 385, 536, 673, 1093, 1172
MluI ACGCGT 1 cut(s) 47
MlyI GAGTC 2 cut(s) 764, 1049
MmeI TCCRAC 2 cut(s) 189, 351
MroI TCCGGA 2 cut(s) 289, 843
MroXI GAANNNNTTC 3 cut(s) 28, 65, 432
MseI TTAA 5 cut(s) 303, 383, 395, 794, 869
MslI CAYNNNNRTG 2 cut(s) 515, 519
MspA1I CMGCKG 2 cut(s) 884, 994
MspCI CTTAAG 1 cut(s) 868
MspI CCGG 3 cut(s) 206, 290, 844
MspR9I CCNGG 1 cut(s) 206
Mva1269I GAATGC 2 cut(s) 667, 909
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 3 cut(s) 229, 232, 887
NciI CCSGG 1 cut(s) 206
NcoI CCATGG 1 cut(s) 265
NdeI CATATG 1 cut(s) 682
NdeII GATC 3 cut(s) 451, 475, 646
NheI GCTAGC 1 cut(s) 943
NlaIII CATG 6 cut(s) 269, 440, 487, 520, 613, 768
NlaIV GGNNCC 1 cut(s) 263
NspI RCATGY 2 cut(s) 487, 520
NspV TTCGAA 1 cut(s) 74
PaeI GCATGC 1 cut(s) 487
PaeR7I CTCGAG 2 cut(s) 106, 914
PagI TCATGA 1 cut(s) 436
PciI ACATGT 1 cut(s) 516
PcsI WCGNNNNNNNCGW 1 cut(s) 562
PctI GAATGC 2 cut(s) 667, 909
PdmI GAANNNNTTC 3 cut(s) 28, 65, 432
PfeI GAWTC 2 cut(s) 407, 1028
PflMI CCANNNNNTGG 2 cut(s) 748, 1136
PkrI GCNGC 7 cut(s) 56, 91, 225, 234, 360, 720, 883
PleI GAGTC 2 cut(s) 763, 1048
PpsI GAGTC 2 cut(s) 763, 1048
PscI ACATGT 1 cut(s) 516
Psp1406I AACGTT 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 263
PspPI GGNCC 2 cut(s) 292, 731
PspXI VCTCGAGB 1 cut(s) 106
PsuI RGATCY 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 884
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
RseI CAYNNNNRTG 2 cut(s) 515, 519
SaqAI TTAA 5 cut(s) 303, 383, 395, 794, 869
SatI GCNGC 7 cut(s) 55, 90, 224, 233, 359, 719, 882
Sau3AI GATC 3 cut(s) 451, 475, 646
Sau96I GGNCC 2 cut(s) 292, 731
SchI GAGTC 2 cut(s) 764, 1049
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 2 cut(s) 188, 206
SfaNI GCATC 2 cut(s) 353, 899
SfcI CTRYAG 2 cut(s) 876, 1150
Sfr274I CTCGAG 2 cut(s) 106, 914
SfuI TTCGAA 1 cut(s) 74
SinI GGWCC 2 cut(s) 292, 731
SlaI CTCGAG 2 cut(s) 106, 914
SmiMI CAYNNNNRTG 2 cut(s) 515, 519
SmlI CTYRAG 3 cut(s) 106, 868, 914
SmoI CTYRAG 3 cut(s) 106, 868, 914
SphI GCATGC 1 cut(s) 487
Sse9I AATT 6 cut(s) 308, 385, 536, 673, 1093, 1172
SsiI CCGC 8 cut(s) 55, 87, 90, 111, 174, 232, 359, 992
SspMI CTAG 2 cut(s) 411, 944
StyD4I CCNGG 1 cut(s) 204
StyI CCWWGG 2 cut(s) 265, 275
TaiI ACGT 3 cut(s) 30, 171, 326
TasI AATT 6 cut(s) 308, 385, 536, 673, 1093, 1172
TauI GCSGC 4 cut(s) 57, 92, 235, 361
TfiI GAWTC 2 cut(s) 407, 1028
Tru1I TTAA 5 cut(s) 303, 383, 395, 794, 869
Tru9I TTAA 5 cut(s) 303, 383, 395, 794, 869
TscAI CASTG 1 cut(s) 255
TseI GCWGC 3 cut(s) 223, 718, 881
TspDTI ATGAA 8 cut(s) 425, 530, 669, 699, 713, 753, 971, 1095
TspGWI ACGGA 2 cut(s) 975, 1040
TspRI CASTG 1 cut(s) 255
Van91I CCANNNNNTGG 2 cut(s) 748, 1136
Vha464I CTTAAG 1 cut(s) 868
VpaK11BI GGWCC 2 cut(s) 292, 731
XapI RAATTY 3 cut(s) 308, 536, 1172
XbaI TCTAGA 1 cut(s) 410
XceI RCATGY 2 cut(s) 487, 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XhoI CTCGAG 2 cut(s) 106, 914
XmnI GAANNNNTTC 3 cut(s) 28, 65, 432
XspI CTAG 2 cut(s) 411, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.