Prupe.2G049500_v2.0.a1

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0003610
Physical Location & Seq
Forward (+)
5666851 .. 5673545
6695 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G049500.4

Sequence Viewer

Length: 1215 bp
ATGGAGAGGCGAGGGCGTGGTCGTCTTCAACCTCGAAACGTTTCTTCTCGAGAATCAACGGTTTCTCGCACGAACAGGTTCGCTTCGAAACCCAACTCGACTCCCAAGTCCTCGAGCAGAGTTGCAAGTGGCGGTGAAAAAGCACAAAACGACGGCGTGGATGACCCTTCCGACCTTCCCGCCATTGTGGGTACTTGCCCTTTGATGTGCCCAGAAAGAGAGAGAGCTCAGCGAGAGCGGCTTCGAGATTTAGCAGTGTTTGAGAGGCTAAATGGAAATCCAGCTCAATCATCTCCAGACCTTGCCGTTAAAAAGTTTTTCAGAACTATATCCACCAAACAAGTTGAAGCATCAGATGTCCGGCCGCTCCCTGTGTTGGAAGACACTTTAAATTATCTTTTAAACTTTTTTGATTCTAGAGAATATCCCTTTGAAGTGGTTCATGACTTCCTCTTTGATCGAACAAGGTCGATAAGGCAAGATCTTAGTATGCAGAATATTGTCAACAATAAAGTAATCCGCATGTATGAGAAAATGGTCAAATTTCATGTGATATGTCTTCACAAGCTTCAGCGTTGTAGCAGTCAAAATACTTCTTCCATGAATTACCTTAACAAGGAGCAGCTTGCAAAGACACTGACATCTCTATTTAATCTGTATGATGCAAATCGAGATTCCAATTCTACATATGAAAATGAAGCTGAGTTTTGTTCGTTTTATGTGCTACTTCATCTTGGTTCTAACAGCCAACCAATGGGGGAGTCACTTTCTTGGTGGTTTCGTAATGTTCCTTCCACGTTGATGAAAACTAAGGAAATTTGCTTTTCTCGAAAGATTTTACGATTTTTCCGGATAGGCAACTATAATTGTTTCCTAAGCACTATAGCAGCTGAGGCATCTTATCTTCAATACTGCATTCTTGAACCTTATGTCAATGAGGTTCGAGCGCTAGCTGTGTCATGTATAAATAATGGTGGATACAAGCTTCATCCATACCCGCTGGCAAACTTATCCAAGCTCTTGATGATGACGGAATCAGATTTGGAATCATTTTGCAAAGCTTGTGGTCTTGAGATTTGTACAAATGAAGAAGGATATAATTTGCTACCTACTAAGCAAACCACCTTTCGTCATCCTAAGGATGGGTTTCAAAGCTATATATTTGTGGGTTTGGAACAATTTGAGAGTGGTACTCTCAAGCCAAAAAGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

405

Amino Acids

46.35

Weight (kDa)

8.97

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 106
AccB7I CCANNNNNTGG 1 cut(s) 754
AccBSI CCGCTC 2 cut(s) 238, 367
AccIII TCCGGA 1 cut(s) 849
AciI CCGC 6 cut(s) 132, 180, 238, 365, 520, 998
AclI AACGTT 1 cut(s) 39
AcoI YGGCCR 1 cut(s) 362
AcsI RAATTY 2 cut(s) 542, 816
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 3 cut(s) 193, 1081, 1192
AfeI AGCGCT 1 cut(s) 948
AfiI CCNNNNNNNGG 4 cut(s) 376, 616, 754, 1142
AgsI TTSAA 6 cut(s) 29, 347, 434, 908, 923, 1151
Alw21I GWGCWC 1 cut(s) 229
Ama87I CYCGRG 2 cut(s) 48, 112
Aor13HI TCCGGA 1 cut(s) 849
Aor51HI AGCGCT 1 cut(s) 948
AoxI GGCC 1 cut(s) 362
ApeKI GCWGC 2 cut(s) 622, 887
ApoI RAATTY 2 cut(s) 542, 816
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
Asp700I GAANNNNTTC 3 cut(s) 40, 77, 438
AspLEI GCGC 1 cut(s) 949
AsuHPI GGTGA 1 cut(s) 146
AsuII TTCGAA 1 cut(s) 86
AsuNHI GCTAGC 1 cut(s) 949
AvaI CYCGRG 2 cut(s) 48, 112
AxyI CCTNAGG 1 cut(s) 1137
BaeGI GKGCMC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 775, 807
BbsI GAAGAC 3 cut(s) 17, 387, 551
Bbv12I GWGCWC 1 cut(s) 229
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 2 cut(s) 634, 899
BccI CCATC 1 cut(s) 1136
BceAI ACGGC 2 cut(s) 169, 290
BciVI GTATCC 1 cut(s) 971
BfaI CTAG 2 cut(s) 417, 950
BfmI CTRYAG 1 cut(s) 882
BfoI RGCGCY 1 cut(s) 950
BfuI GTATCC 1 cut(s) 971
BglII AGATCT 1 cut(s) 481
BisI GCNGC 4 cut(s) 239, 365, 623, 888
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 4 cut(s) 240, 366, 624, 889
BmeT110I CYCGRG 2 cut(s) 48, 112
BmsI GCATC 3 cut(s) 359, 652, 905
BmtI GCTAGC 1 cut(s) 953
BpiI GAAGAC 3 cut(s) 17, 387, 551
BplI GAGNNNNNCTC 2 cut(s) 1177, 1209
BpmI CTGGAG 1 cut(s) 279
Bpu10I CCTNAGC 2 cut(s) 875, 891
Bpu1102I GCTNAGC 1 cut(s) 228
Bpu14I TTCGAA 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 1091, 1181
BsaBI GATNNNNATC 1 cut(s) 666
BsaWI WCCGGW 1 cut(s) 849
Bsc4I CCNNNNNNNGG 4 cut(s) 376, 616, 754, 1142
Bse21I CCTNAGG 1 cut(s) 1137
Bse8I GATNNNNATC 1 cut(s) 666
BseAI TCCGGA 1 cut(s) 849
BseGI GGATG 4 cut(s) 166, 988, 1132, 1147
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 4 cut(s) 376, 616, 754, 1142
BseMII CTCAG 3 cut(s) 242, 693, 882
BseSI GKGCMC 1 cut(s) 212
BseX3I CGGCCG 1 cut(s) 362
BseXI GCAGC 2 cut(s) 634, 899
Bsh1285I CGRYCG 1 cut(s) 365
BshFI GGCC 1 cut(s) 364
BsiEI CGRYCG 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 229
BsiHKCI CYCGRG 2 cut(s) 48, 112
BsiSI CCGG 2 cut(s) 361, 850
BslI CCNNNNNNNGG 4 cut(s) 376, 616, 754, 1142
BsmI GAATGC 1 cut(s) 915
BsnI GGCC 1 cut(s) 364
BsoBI CYCGRG 2 cut(s) 48, 112
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 212, 229
Bsp13I TCCGGA 1 cut(s) 849
Bsp1407I TGTACA 1 cut(s) 1079
Bsp143I GATC 2 cut(s) 457, 481
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 6 cut(s) 132, 180, 238, 365, 520, 998
BspANI GGCC 1 cut(s) 364
BspCNI CTCAG 3 cut(s) 241, 694, 883
BspEI TCCGGA 1 cut(s) 849
BspHI TCATGA 1 cut(s) 442
BspOI GCTAGC 1 cut(s) 953
BspT104I TTCGAA 1 cut(s) 86
BsrBI CCGCTC 2 cut(s) 238, 367
BsrGI TGTACA 1 cut(s) 1079
BssMI GATC 2 cut(s) 457, 481
Bst4CI ACNGT 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 1079
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 3 cut(s) 627, 951, 1002
BstDEI CTNAG 8 cut(s) 228, 485, 702, 810, 875, 891, 1113, 1137
BstENI CCTNNNNNAGG 1 cut(s) 614
BstF5I GGATG 4 cut(s) 166, 988, 1132, 1147
BstH2I RGCGCY 1 cut(s) 950
BstHHI GCGC 1 cut(s) 949
BstKTI GATC 2 cut(s) 460, 484
BstMBI GATC 2 cut(s) 457, 481
BstMCI CGRYCG 1 cut(s) 365
BstMWI GCNNNNNNNGC 2 cut(s) 238, 893
BstNSI RCATGY 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 882
BstSLI GKGCMC 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 634, 899
BstV2I GAAGAC 3 cut(s) 17, 387, 551
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 1 cut(s) 362
Bsu36I CCTNAGG 1 cut(s) 1137
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 1 cut(s) 364
BtsCI GGATG 4 cut(s) 166, 988, 1132, 1147
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 2 cut(s) 261, 635
Cac8I GCNNGC 3 cut(s) 627, 951, 1002
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 949
Csp6I GTAC 3 cut(s) 192, 1080, 1191
CspCI CAANNNNNGTGG 2 cut(s) 1045, 1080
CviAII CATG 5 cut(s) 443, 523, 548, 601, 960
CviQI GTAC 3 cut(s) 192, 1080, 1191
DdeI CTNAG 8 cut(s) 228, 485, 702, 810, 875, 891, 1113, 1137
DpnI GATC 2 cut(s) 459, 483
DpnII GATC 2 cut(s) 457, 481
DraI TTTAAA 2 cut(s) 390, 402
DrdI GACNNNNNNGTC 1 cut(s) 106
DseDI GACNNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 362
EagI CGGCCG 1 cut(s) 362
Ecl136II GAGCTC 1 cut(s) 227
EclXI CGGCCG 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 229
Eco47III AGCGCT 1 cut(s) 948
Eco52I CGGCCG 1 cut(s) 362
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 554
Eco81I CCTNAGG 1 cut(s) 1137
Eco88I CYCGRG 2 cut(s) 48, 112
EcoICRI GAGCTC 1 cut(s) 227
EcoNI CCTNNNNNAGG 1 cut(s) 614
EcoT38I GRGCYC 1 cut(s) 229
FaeI CATG 5 cut(s) 446, 526, 551, 604, 963
FatI CATG 5 cut(s) 442, 522, 547, 600, 959
FauI CCCGC 2 cut(s) 187, 1005
FauNDI CATATG 1 cut(s) 688
Fnu4HI GCNGC 4 cut(s) 239, 365, 623, 888
FokI GGATG 4 cut(s) 173, 975, 1119, 1154
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 4 cut(s) 239, 365, 623, 888
FspBI CTAG 2 cut(s) 417, 950
GlaI GCGC 1 cut(s) 948
GluI GCNGC 4 cut(s) 239, 365, 623, 888
GsuI CTGGAG 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 950
HaeIII GGCC 1 cut(s) 364
HapII CCGG 2 cut(s) 361, 850
HhaI GCGC 1 cut(s) 949
Hin1II CATG 5 cut(s) 446, 526, 551, 604, 963
Hin6I GCGC 1 cut(s) 947
HinP1I GCGC 1 cut(s) 947
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 3 cut(s) 566, 983, 1059
HinfI GANTC 7 cut(s) 53, 100, 413, 674, 761, 1034, 1046
HpaII CCGG 2 cut(s) 361, 850
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 4 cut(s) 172, 323, 355, 1039
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 4 cut(s) 177, 185, 801, 1085
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4IV ACGT 2 cut(s) 39, 797
HpyCH4V TGCA 6 cut(s) 125, 493, 629, 665, 915, 1056
HpyF10VI GCNNNNNNNGC 2 cut(s) 238, 893
HpyF3I CTNAG 8 cut(s) 228, 485, 702, 810, 875, 891, 1113, 1137
HpySE526I ACGT 2 cut(s) 39, 797
Hsp92II CATG 5 cut(s) 446, 526, 551, 604, 963
HspAI GCGC 1 cut(s) 947
Kpn2I TCCGGA 1 cut(s) 849
Kzo9I GATC 2 cut(s) 457, 481
LmnI GCTCC 2 cut(s) 372, 619
LpnPI CCDG 8 cut(s) 61, 225, 294, 309, 374, 384, 863, 986
Lsp1109I GCAGC 2 cut(s) 634, 899
LweI GCATC 3 cut(s) 359, 652, 905
MaeI CTAG 2 cut(s) 417, 950
MaeII ACGT 2 cut(s) 39, 797
MaeIII GTNAC 1 cut(s) 762
MalI GATC 2 cut(s) 459, 483
MbiI CCGCTC 2 cut(s) 238, 367
MboI GATC 2 cut(s) 457, 481
MboII GAAGA 7 cut(s) 17, 36, 392, 551, 588, 896, 1100
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 2 cut(s) 212, 229
MluCI AATT 8 cut(s) 391, 542, 604, 679, 816, 865, 1099, 1178
MlyI GAGTC 2 cut(s) 94, 770
MmeI TCCRAC 2 cut(s) 195, 357
MnlI CCTC 7 cut(s) 5, 42, 121, 258, 461, 886, 931
MroI TCCGGA 1 cut(s) 849
MroXI GAANNNNTTC 3 cut(s) 40, 77, 438
MseI TTAA 5 cut(s) 309, 389, 401, 612, 651
MslI CAYNNNNRTG 1 cut(s) 800
MspA1I CMGCKG 2 cut(s) 890, 1000
MspI CCGG 2 cut(s) 361, 850
Mva1269I GAATGC 1 cut(s) 915
MwoI GCNNNNNNNGC 2 cut(s) 238, 893
NdeI CATATG 1 cut(s) 688
NdeII GATC 2 cut(s) 457, 481
NheI GCTAGC 1 cut(s) 949
NlaIII CATG 5 cut(s) 446, 526, 551, 604, 963
NmuCI GTSAC 1 cut(s) 762
NspI RCATGY 1 cut(s) 526
NspV TTCGAA 1 cut(s) 86
PaeR7I CTCGAG 2 cut(s) 48, 112
PagI TCATGA 1 cut(s) 442
PctI GAATGC 1 cut(s) 915
PdmI GAANNNNTTC 3 cut(s) 40, 77, 438
PfeI GAWTC 5 cut(s) 53, 413, 674, 1034, 1046
PflMI CCANNNNNTGG 1 cut(s) 754
PkrI GCNGC 4 cut(s) 240, 366, 624, 889
PleI GAGTC 2 cut(s) 94, 769
PpsI GAGTC 2 cut(s) 94, 769
Psp124BI GAGCTC 1 cut(s) 229
Psp1406I AACGTT 1 cut(s) 39
PspXI VCTCGAGB 1 cut(s) 112
PsuI RGATCY 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 890
RsaI GTAC 3 cut(s) 193, 1081, 1192
RsaNI GTAC 3 cut(s) 192, 1080, 1191
RseI CAYNNNNRTG 1 cut(s) 800
SacI GAGCTC 1 cut(s) 229
SaqAI TTAA 5 cut(s) 309, 389, 401, 612, 651
SatI GCNGC 4 cut(s) 239, 365, 623, 888
Sau3AI GATC 2 cut(s) 457, 481
SchI GAGTC 2 cut(s) 94, 770
SduI GDGCHC 2 cut(s) 212, 229
SfaNI GCATC 3 cut(s) 359, 652, 905
SfcI CTRYAG 1 cut(s) 882
Sfr274I CTCGAG 2 cut(s) 48, 112
SfuI TTCGAA 1 cut(s) 86
SlaI CTCGAG 2 cut(s) 48, 112
SmiMI CAYNNNNRTG 1 cut(s) 800
SmlI CTYRAG 4 cut(s) 48, 112, 1070, 1196
SmoI CTYRAG 4 cut(s) 48, 112, 1070, 1196
Sse9I AATT 8 cut(s) 391, 542, 604, 679, 816, 865, 1099, 1178
SsiI CCGC 6 cut(s) 132, 180, 238, 365, 520, 998
SspI AATATT 1 cut(s) 499
SspMI CTAG 2 cut(s) 417, 950
SstI GAGCTC 1 cut(s) 229
TaaI ACNGT 1 cut(s) 61
TaiI ACGT 2 cut(s) 42, 800
TasI AATT 8 cut(s) 391, 542, 604, 679, 816, 865, 1099, 1178
TatI WGTACW 1 cut(s) 1079
TauI GCSGC 2 cut(s) 241, 367
TfiI GAWTC 5 cut(s) 53, 413, 674, 1034, 1046
Tru1I TTAA 5 cut(s) 309, 389, 401, 612, 651
Tru9I TTAA 5 cut(s) 309, 389, 401, 612, 651
TscAI CASTG 2 cut(s) 261, 642
TseFI GTSAC 1 cut(s) 762
TseI GCWGC 2 cut(s) 622, 887
Tsp45I GTSAC 1 cut(s) 762
TspDTI ATGAA 9 cut(s) 431, 536, 617, 705, 711, 719, 818, 977, 1101
TspGWI ACGGA 1 cut(s) 1046
TspRI CASTG 2 cut(s) 261, 642
Van91I CCANNNNNTGG 1 cut(s) 754
XagI CCTNNNNNAGG 1 cut(s) 614
XapI RAATTY 2 cut(s) 542, 816
XbaI TCTAGA 1 cut(s) 416
XceI RCATGY 1 cut(s) 526
XhoI CTCGAG 2 cut(s) 48, 112
XmnI GAANNNNTTC 3 cut(s) 40, 77, 438
XspI CTAG 2 cut(s) 417, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.