Rroxscaffold_4G00321090

SAC3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003610
Physical Location & Seq
Forward (+)
50250438 .. 50257751
7314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321090.1

Sequence Viewer

Length: 1149 bp
ATGGAGAGAAGAGGCCGTCGAAACCGTTCTTCTTATTCTTCTTCTTCTTCTTCTTCTTCTCGGGGTTCAAGATCCTGGAGCAGAGGTGGAACTAGTTCCGCCGAGAAAGTGGAAAACGTCGCCGTTTCCGATGTTCCCGCCATAGTGGGTACTTGCACTTTGATGTGCCCAGAAGCAGAAAGGGCACAGCGGGAGCGCCTTCGCGATTTAGCTACCTTTGAGAGGCTAAATGGAAATCCAGCTCGCTCATCGCCATCCATTGCAGTTAAAAAGTTTTTCAGAACTATGTCCACCAAACAAGTTCAAGCATCAGATGTTCGGCCGCTCCCTGTTTTGGAGGATACTTTAAATTACCTCTTAGAGTTGTTTGATTCTAAAGACTATCCTTTTGAAGTGATTCACGACTTCCTCTTTGATCGAACAAGGTCCATAAGGCAAGATCTTAGCATGCAGAATATTGGTGGCGAGAAAGCAATTCACATGTATCAGGAAATGGTTAAATTTCATATCATCTCTCTTCATAAGCTTCGAGGTTGCAGCAGTCAAAATACTTCTCCAATGCATCACCTCAACATGGAGCAGCTTGCAAAGACCTTGACATCTCTATTTAATCTGTATGGCGCAAATCGTCATTCCAATTCTACTAATGAAAATGAGGCTGAGTTTTGTTCTTTTTATGTGCTACTTCATCTTGGTTCTCATAGCCAACCCCTGGGGGAGTCACTTTCTTGGTGGTTTAGTCATGTGCCTTCTTCGATAATCAAGTCAAAGGAAATGCATTTTTCCCGAAAGATTTTACGATATTTTCGGATGGGTAACTATAAATGTTTCTTAAGTACTGTAGCAGCTGAGGCATCTTATCTTCAATACTGCATTATTGAGCCTTATGTAGATGAGGTTCGAGTTTTAGCTGTGTCATGCATCAATAGTGGTGGCTACAAGCTTCATCCATATCCTCTGTCGCATTTATCCAAACTCTTGATTATGAAGGAATCAGATTTGGAATCTTTTCTCAAATCCTGTGGTCTTCAAATTTGCACTGATGAAGAAGGACATAATCTGTTGCCCACTAAGCAAACGACCTTTTGTCAACCCAAGGATGGGTCTCAAAACTACAGGTTTTGGGGTATGGAACAATTTGATAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

382

Amino Acids

43.59

Weight (kDa)

8.59

Isoelectric Point (pI)

57.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 55 - 345 3.1e-79 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 712
AccBSI CCGCTC 1 cut(s) 325
AccII CGCG 1 cut(s) 204
AciI CCGC 4 cut(s) 99, 138, 190, 323
AclWI GGATC 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 320
AcsI RAATTY 2 cut(s) 500, 1032
AfaI GTAC 2 cut(s) 151, 838
AfiI CCNNNNNNNGG 6 cut(s) 222, 334, 574, 712, 1100, 1101
AflII CTTAAG 1 cut(s) 832
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 5 cut(s) 69, 305, 392, 866, 1031
AhdI GACNNNNNGTC 1 cut(s) 1086
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 2 cut(s) 74, 711
AluBI AGCT 8 cut(s) 212, 242, 526, 583, 848, 911, 943, 1146
AluI AGCT 8 cut(s) 212, 242, 526, 583, 848, 911, 943, 1146
Alw26I GTCTC 1 cut(s) 1110
AlwI GGATC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 60
AoxI GGCC 2 cut(s) 13, 320
ApeKI GCWGC 3 cut(s) 537, 580, 845
ApoI RAATTY 2 cut(s) 500, 1032
ArsI GACNNNNNNTTYG 4 cut(s) 371, 395, 403, 427
Asp700I GAANNNNTTC 4 cut(s) 25, 94, 396, 1008
AspLEI GCGC 2 cut(s) 198, 623
AspS9I GGNCC 1 cut(s) 426
AsuHPI GGTGA 1 cut(s) 557
AvaI CYCGRG 1 cut(s) 60
AvaII GGWCC 1 cut(s) 426
BaeGI GKGCMC 2 cut(s) 170, 187
BbsI GAAGAC 1 cut(s) 1019
BbvCI CCTCAGC 1 cut(s) 849
BbvI GCAGC 3 cut(s) 549, 592, 857
BccI CCATC 3 cut(s) 262, 805, 1094
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 2 cut(s) 76, 713
BciVI GTATCC 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 1110
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 2 cut(s) 840, 1114
BfoI RGCGCY 1 cut(s) 199
BfrI CTTAAG 1 cut(s) 832
BfuI GTATCC 1 cut(s) 334
BglII AGATCT 1 cut(s) 439
BisI GCNGC 4 cut(s) 323, 538, 581, 846
BlsI GCNGC 4 cut(s) 324, 539, 582, 847
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 2 cut(s) 76, 713
Bme18I GGWCC 1 cut(s) 426
BmeRI GACNNNNNGTC 1 cut(s) 1086
BmeT110I CYCGRG 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 426
BmrFI CCNGG 2 cut(s) 76, 713
BmsI GCATC 4 cut(s) 317, 571, 863, 930
BpiI GAAGAC 1 cut(s) 1019
BpmI CTGGAG 1 cut(s) 97
Bpu10I CCTNAGC 1 cut(s) 849
BsaI GGTCTC 1 cut(s) 1110
BsaJI CCNNGG 3 cut(s) 711, 712, 1095
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 6 cut(s) 222, 334, 574, 712, 1100, 1101
Bse3DI GCAATG 1 cut(s) 258
BseBI CCWGG 2 cut(s) 76, 713
BseDI CCNNGG 3 cut(s) 711, 712, 1095
BseGI GGATG 4 cut(s) 254, 816, 946, 1105
BseLI CCNNNNNNNGG 6 cut(s) 222, 334, 574, 712, 1100, 1101
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 651, 840
BseSI GKGCMC 2 cut(s) 170, 187
BseX3I CGGCCG 1 cut(s) 320
BseXI GCAGC 3 cut(s) 549, 592, 857
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 323
BshFI GGCC 2 cut(s) 15, 322
BsiEI CGRYCG 1 cut(s) 323
BsiHKCI CYCGRG 1 cut(s) 60
BslI CCNNNNNNNGG 6 cut(s) 222, 334, 574, 712, 1100, 1101
BsmAI GTCTC 1 cut(s) 1110
BsnI GGCC 2 cut(s) 15, 322
Bso31I GGTCTC 1 cut(s) 1110
BsoBI CYCGRG 1 cut(s) 60
Bsp1286I GDGCHC 2 cut(s) 170, 187
Bsp143I GATC 3 cut(s) 71, 415, 439
Bsp68I TCGCGA 1 cut(s) 204
BspACI CCGC 4 cut(s) 99, 138, 190, 323
BspANI GGCC 2 cut(s) 15, 322
BspCNI CTCAG 2 cut(s) 652, 841
BspFNI CGCG 1 cut(s) 204
BspPI GGATC 1 cut(s) 66
BspTI CTTAAG 1 cut(s) 832
BspTNI GGTCTC 1 cut(s) 1110
BsrBI CCGCTC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 258
BssECI CCNNGG 3 cut(s) 711, 712, 1095
BssMI GATC 3 cut(s) 71, 415, 439
BssT1I CCWWGG 1 cut(s) 1095
Bst2UI CCWGG 2 cut(s) 76, 713
Bst4CI ACNGT 2 cut(s) 26, 841
Bst6I CTCTTC 2 cut(s) 4, 522
BstAFI CTTAAG 1 cut(s) 832
BstC8I GCNNGC 3 cut(s) 244, 449, 585
BstDEI CTNAG 5 cut(s) 358, 443, 660, 849, 1071
BstENI CCTNNNNNAGG 1 cut(s) 220
BstF5I GGATG 4 cut(s) 254, 816, 946, 1105
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 2 cut(s) 198, 623
BstKTI GATC 3 cut(s) 74, 418, 442
BstMAI GTCTC 1 cut(s) 1110
BstMBI GATC 3 cut(s) 71, 415, 439
BstMCI CGRYCG 1 cut(s) 323
BstMWI GCNNNNNNNGC 3 cut(s) 182, 851, 1072
BstNI CCWGG 2 cut(s) 76, 713
BstNSI RCATGY 2 cut(s) 451, 484
BstSCI CCNGG 2 cut(s) 74, 711
BstSFI CTRYAG 2 cut(s) 840, 1114
BstSLI GKGCMC 2 cut(s) 170, 187
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 3 cut(s) 549, 592, 857
BstV2I GAAGAC 1 cut(s) 1019
BstX2I RGATCY 2 cut(s) 71, 439
BstYI RGATCY 2 cut(s) 71, 439
BstZI CGGCCG 1 cut(s) 320
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 2 cut(s) 15, 322
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 4 cut(s) 254, 816, 946, 1105
BtsIMutI CAGTG 1 cut(s) 1038
BtuMI TCGCGA 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 244, 449, 585
CfoI GCGC 2 cut(s) 198, 623
Cfr13I GGNCC 1 cut(s) 426
Csp6I GTAC 2 cut(s) 150, 837
CspCI CAANNNNNGTGG 4 cut(s) 913, 948, 1003, 1038
CviAII CATG 5 cut(s) 448, 481, 574, 743, 918
CviQI GTAC 2 cut(s) 150, 837
DdeI CTNAG 5 cut(s) 358, 443, 660, 849, 1071
DpnI GATC 3 cut(s) 73, 417, 441
DpnII GATC 3 cut(s) 71, 415, 439
DraI TTTAAA 1 cut(s) 348
DriI GACNNNNNGTC 1 cut(s) 1086
EaeI YGGCCR 1 cut(s) 320
EagI CGGCCG 1 cut(s) 320
Eam1104I CTCTTC 2 cut(s) 4, 522
Eam1105I GACNNNNNGTC 1 cut(s) 1086
EarI CTCTTC 2 cut(s) 4, 522
EciI GGCGGA 1 cut(s) 88
EclXI CGGCCG 1 cut(s) 320
Eco130I CCWWGG 1 cut(s) 1095
Eco31I GGTCTC 1 cut(s) 1110
Eco47I GGWCC 1 cut(s) 426
Eco52I CGGCCG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 60
EcoNI CCTNNNNNAGG 1 cut(s) 220
EcoRII CCWGG 2 cut(s) 74, 711
EcoT14I CCWWGG 1 cut(s) 1095
EcoT22I ATGCAT 3 cut(s) 564, 780, 923
ErhI CCWWGG 1 cut(s) 1095
FaeI CATG 5 cut(s) 451, 484, 577, 746, 921
FatI CATG 5 cut(s) 447, 480, 573, 742, 917
FauI CCCGC 2 cut(s) 145, 183
Fnu4HI GCNGC 4 cut(s) 323, 538, 581, 846
FokI GGATG 4 cut(s) 241, 823, 933, 1112
Fsp4HI GCNGC 4 cut(s) 323, 538, 581, 846
FspBI CTAG 1 cut(s) 93
GlaI GCGC 2 cut(s) 197, 622
GluI GCNGC 4 cut(s) 323, 538, 581, 846
GsuI CTGGAG 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 2 cut(s) 15, 322
HhaI GCGC 2 cut(s) 198, 623
Hin1II CATG 5 cut(s) 451, 484, 577, 746, 921
Hin6I GCGC 2 cut(s) 196, 621
HinP1I GCGC 2 cut(s) 196, 621
HincII GTYRAC 1 cut(s) 1091
HindII GTYRAC 1 cut(s) 1091
HindIII AAGCTT 2 cut(s) 524, 941
HinfI GANTC 5 cut(s) 371, 397, 719, 992, 1004
HphI GGTGA 1 cut(s) 557
Hpy166II GTNNAC 2 cut(s) 291, 1091
Hpy188I TCNGA 5 cut(s) 130, 281, 313, 810, 997
Hpy188III TCNNGA 6 cut(s) 69, 203, 401, 488, 786, 979
Hpy8I GTNNAC 2 cut(s) 291, 1091
Hpy99I CGWCG 2 cut(s) 21, 122
HpyAV CCTTC 4 cut(s) 209, 759, 982, 1043
HpyCH4III ACNGT 2 cut(s) 26, 841
HpyCH4IV ACGT 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 851, 1072
HpyF3I CTNAG 5 cut(s) 358, 443, 660, 849, 1071
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 5 cut(s) 451, 484, 577, 746, 921
HspAI GCGC 2 cut(s) 196, 621
Kzo9I GATC 3 cut(s) 71, 415, 439
LmnI GCTCC 4 cut(s) 78, 193, 330, 577
Lsp1109I GCAGC 3 cut(s) 549, 592, 857
LweI GCATC 4 cut(s) 317, 571, 863, 930
MaeI CTAG 1 cut(s) 93
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 720, 815
MalI GATC 3 cut(s) 73, 417, 441
MbiI CCGCTC 1 cut(s) 325
MboI GATC 3 cut(s) 71, 415, 439
MflI RGATCY 2 cut(s) 71, 439
MhlI GDGCHC 2 cut(s) 170, 187
MluCI AATT 6 cut(s) 349, 474, 500, 637, 1032, 1136
MlyI GAGTC 1 cut(s) 728
Mph1103I ATGCAT 3 cut(s) 564, 780, 923
MroXI GAANNNNTTC 4 cut(s) 25, 94, 396, 1008
MseI TTAA 5 cut(s) 267, 347, 498, 609, 833
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 2 cut(s) 190, 848
MspCI CTTAAG 1 cut(s) 832
MspR9I CCNGG 2 cut(s) 76, 713
MvaI CCWGG 2 cut(s) 76, 713
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 3 cut(s) 182, 851, 1072
NdeII GATC 3 cut(s) 71, 415, 439
NlaIII CATG 5 cut(s) 451, 484, 577, 746, 921
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 720
NruI TCGCGA 1 cut(s) 204
NsiI ATGCAT 3 cut(s) 564, 780, 923
NspI RCATGY 2 cut(s) 451, 484
PaeI GCATGC 1 cut(s) 451
PasI CCCWGGG 1 cut(s) 712
PciI ACATGT 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdmI GAANNNNTTC 4 cut(s) 25, 94, 396, 1008
PfeI GAWTC 4 cut(s) 371, 397, 992, 1004
PflMI CCANNNNNTGG 1 cut(s) 712
PfoI TCCNGGA 1 cut(s) 74
PkrI GCNGC 4 cut(s) 324, 539, 582, 847
PleI GAGTC 1 cut(s) 727
PpsI GAGTC 1 cut(s) 727
PscI ACATGT 1 cut(s) 480
Psp6I CCWGG 2 cut(s) 74, 711
PspGI CCWGG 2 cut(s) 74, 711
PspPI GGNCC 1 cut(s) 426
PsrI GAACNNNNNNTAC 2 cut(s) 882, 914
PsuI RGATCY 2 cut(s) 71, 439
PvuII CAGCTG 1 cut(s) 848
RruI TCGCGA 1 cut(s) 204
RsaI GTAC 2 cut(s) 151, 838
RsaNI GTAC 2 cut(s) 150, 837
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 5 cut(s) 267, 347, 498, 609, 833
SatI GCNGC 4 cut(s) 323, 538, 581, 846
Sau3AI GATC 3 cut(s) 71, 415, 439
Sau96I GGNCC 1 cut(s) 426
ScaI AGTACT 1 cut(s) 838
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 2 cut(s) 76, 713
SduI GDGCHC 2 cut(s) 170, 187
SfaNI GCATC 4 cut(s) 317, 571, 863, 930
SfcI CTRYAG 2 cut(s) 840, 1114
SinI GGWCC 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 832
SmoI CTYRAG 1 cut(s) 832
SpeI ACTAGT 1 cut(s) 92
SphI GCATGC 1 cut(s) 451
Sse9I AATT 6 cut(s) 349, 474, 500, 637, 1032, 1136
SsiI CCGC 4 cut(s) 99, 138, 190, 323
SspI AATATT 1 cut(s) 457
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 2 cut(s) 74, 711
StyI CCWWGG 1 cut(s) 1095
TaaI ACNGT 2 cut(s) 26, 841
TaiI ACGT 1 cut(s) 120
TaqI TCGA 5 cut(s) 19, 418, 529, 755, 901
TasI AATT 6 cut(s) 349, 474, 500, 637, 1032, 1136
TatI WGTACW 1 cut(s) 836
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 4 cut(s) 371, 397, 992, 1004
Tru1I TTAA 5 cut(s) 267, 347, 498, 609, 833
Tru9I TTAA 5 cut(s) 267, 347, 498, 609, 833
TscAI CASTG 1 cut(s) 1045
TseFI GTSAC 1 cut(s) 720
TseI GCWGC 3 cut(s) 537, 580, 845
Tsp45I GTSAC 1 cut(s) 720
TspDTI ATGAA 7 cut(s) 494, 509, 663, 677, 935, 1001, 1059
TspRI CASTG 1 cut(s) 1045
Van91I CCANNNNNTGG 1 cut(s) 712
Vha464I CTTAAG 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 426
XagI CCTNNNNNAGG 1 cut(s) 220
XapI RAATTY 2 cut(s) 500, 1032
XceI RCATGY 2 cut(s) 451, 484
XmnI GAANNNNTTC 4 cut(s) 25, 94, 396, 1008
XspI CTAG 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 838
Zsp2I ATGCAT 3 cut(s) 564, 780, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.