Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006326

Physical Location & Seq

Genomic Coordinates Forward (+)

20235506 .. 20244046

Gene Structure 8541 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G54670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3657 bp

ATGCCTGCGATACAATCCCCATCGGGGAAGATCTTGCAGCTTGAGATGGAGAATTTCAAATCTTACAAGGGTCATCAATTGGTTGGTCCTTTCAAGGATTTCACTGCTATTATTGGTCCCAACGGTTCTGGCAAATCTAACCTAATGGACGCTATTAGCTTCGTCCTCGGCGTTCGTACCGGTCAACTTCGTGGTTCTCAGCTTAAGGATCTTATCTACGCTTTTGATGATCGTGACAAGGAACAGAGAGGTCGTAAAGCTTTTGTTCGACTCGTTTATCAGATGGACGATGGAGTAGAGCTTCGTTTCACTCGTTCTATTACTAGCGCTGGTGGGAGTGAGTACCGTATTGATAATAGGGTTGTGAATTTGGATGAGTACAATGGGAAGCTACGATCTCTTGGGATACTTGTTAAGGCTCGTAATTTCTTGGTCTTTCAGGGTGATGTTGAATCTATTGCTTCGAAGAATCCAAAAGAACTTACTGGACTCCTCGAGGAGATTTCTGGTTCCGAGGAGCTTAAGAAAGAGTATGAAGGACTAGAAGAAAAGAAGGCCAGCGCTGAAGAAAAAGCAGCTCTTATATACCAGAAGAAGAAAACAATTGGAAATGAGAAGAAGCTGAAGAAAGCACAAAAGGAAGAAGCTGAGAAGCATCTAAGGCTGCAGGAAGAACTGAAAGCTCTGAAGAGAGAACGCTTCCTATGGCAATTATATAACATTGAAAATGATATCGAGAAGGCGAATGAGGATGTTGATTCTGAAAAGAGCAATCGTAAAGATGTCATGAGAGAGCTGGAAAAGTTTGAACGTGAGGCAGGTAAGAGAAAGGTAGAGCAAGCAAAGTATCTGAAAGAAATTGCTCAACGTGAGAAGAAGATTGCTGAGAAAAGCTCGAAGCTTGGAAAAATTCAACCTGAGCTTCTGAGATTTAAGGAGGAGATAGCTCGCATAAAAGCAAAAATTGAGACCAATAGAAAGGACGTGGATAAGAGGAAAAAAGAAAAAGGGAAGCATTCCAAAGAAATTGAACAGATGCAGAAAAGCATTAAGGAGCTGAATAAGAAAATGGAATTATTTAATAAGAAGAGACAAGATAGCAGCGGAAAACTCCCAATGCTTGACAGTCAACTGCAAGATTATTTTCGACTAAAAGAAGAAGCTGGAATGAAAACCATTAAGCTAAGAGATGAGCACGAGGTTCTAGAGAGGCAACGGCGTACCGATCTTGAAGCATTGAGAAATCTGGAAGAAAATTACCAGCAACTAATAAATAGGAAGAATGATCTTGATGAACAAATTAAGCGATTTAAAGACAGGCAAGGAGAGATTGAAACATCTTCGTCGAAATATAAGAATGAAACAACTAGTCTGAAAACGGAGTTACGTGCACTGCAAGAGAAGCACGTAAATGCCAGGGAGGCCTCTGCGAAGTTGAAGACTAGAATCGCGGAATTAGAAGACCAGTTAAGTGATTTAACGGCTGAAAGGTATGAAAATGAGAGAGATAGTAGGCTAACACAGGCTGTTGAGTCTCTCAAGCGTCTATTTCAAGGAGTTCATGGTCGGATGACTGATCTTTGTCGGCCAAATCGGAAGAAGTATAACCTTGCTGTCACTGTTGCCATGGGAAGATTTATGGATGCAGTAGTTGTAGAAGATGAAAACACGGGAAAGGATTGCATCAAGTACTTGAAAGAGCAAAGGCTTCCTCCTATGACATTTATTCCTCTTCAGTCAGTACGTGTCAAGCAAGTGTTTGAAAGATTGAGAAATTTGGGTGGCACAGCAAAGCTGGTGTTCGATGTTATCCAGTTCGATCCTGAATTGGAGAAGGCGGTTCTCTATGCTGTTGGAAATACTCTTGTCTGCGACGAACTTGAAGAAGCCAAGGTTCTTAGCTGGAGCGGAGAGAGATTTAAAGTTGTGACTGTTGATGGTATATTACTCACAAAGGCGGGAACAATGACTGGTGGTACAAGTGGTGGAATGGAAGCAAAGTCTAATAAATGGGATGATAAGAAAATTGAAGGTTTGAAGAAAAATAAAGAAGATTTCGAACAACAACTAGAAAATATTGGATCTATTCGAGAAATGCAAATGAAAGAGTCTGAAATATCGGGTAAAATAAGTGGACTTGAAAAAAAGATTCAGTATGCTGAAATTGAGAAGAAAAGTATCAAAGACAAACTTCCACAGCTTGAGCAAGAGGAGCGGAATATCATTGAAGAAATTGATCGCATAAAACCTGAACTCTCTAAGGCAAGAACTGAGGTCGATAAGAGAAAGACAGAAATGAATAAGTTGGAGAAAAGAATGAATGAGATCGTGGACCGTATCTATAAGGATTTTAGCCAGTCTGTTGGGGTTCCAAACATACGTGTATATGAAGAAACTCAGCTCAAGACTGCTGAAAAGGAGGCTGAAGAAAGGCTGGAACTGAGTAACCAGCTTGCTAAGTTAAAATACCAGCTGGAATATGAGCAAAACCGTGATGTGGGGTCACGGATTCGAAAGATAGAGTCTTCTATCAGTTCTCTGGAAACTGATTTGGAAGGTATACAGAAGACAATGTCTGAACGTAAGGAGACAGCGGTAAAAATAACTAATGAAATCAACAATTGGAAGAAGGAAATGGAAGAGTGCAAACAAAAGTCGGAGGAGTATGAGAAGGAAATCCTGGATTGGAAAAAGCAGGCTTCTCAAGCGACAACAAGCATAACCAAGCTCAATCGTCAAATACATTCGAAGGAAACACAAATTGAGCAGCTGATCTCACAGAAACAGGAAATAACTGAGAAGTGTGAGCTCGAACATATTACCCTTCCTGTCTTATCAGATGCTATGGAGGAAGATGATTCAGATGGGCCACAGTTTGACTTTAGTGAGCTGGGTAGAGCTTATCTCCAAGAACGGAGACCATCTGCTCGTGAAAAAGTGGAGGCAGAATTTAGGCAGAAAATAGAATCTAAAACATCCGAGATTGAAAGAACGGCTCCAAATTTGAGAGCGCTTGACCAGTATGAGGCTATACAAGAGAAAGAAAAACAAGTTAGCCAGGAGTTTGAAGCGGCCAGGAAAGAGGAGAAGCAAGTAGCGGATGCCTTTAATACTGTTAAGCAGAAGAGGTATGAGCTATTTATGGAAGCTTTCAACCACATTGCTAGCAACATTGACAAGATCTATAAGCAACTAACCAAGAGTAATACGCACCCACTGGGTGGGACTGCTTATCTAAACCTAGAAAATGAGGATGATCCGTTTCTACATGGTATAAAGTATACTACAATGCCCCCAACAAAGCGATTTCGTGACATGGAACAGCTCTCTGGAGGAGAGAAAACAGTTGCGGCCTTGGCGTTGCTCTTCTCCATACATAGCTATAGGCCTTCACCCTTTTTCATACTTGATGAAGTGGATGCTGCGCTTGATAATCTAAACGTGGCAAAAGTTGCTAAATTCATTCGTAGCAAGTCATGCCAAGCCGCACGTGACAATCAGGATGCAGAGGATGGGAATGGCTTCCAAAGCATTGTAATATCTCTCAAGGACAGCTTCTATGACAAGGCGGAGGCTCTGGTTGGAGTTTACAGAGACACCGAGAGGAGTTGCTCAAGTACAATGTCATTTGACCTTAGGAACTACCAAGAATCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000793 GO:0000819 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0008150 GO:0008278 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0022402 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044444 GO:0044446 GO:0044464 GO:0051276 GO:0071840 GO:0098813

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1218

Amino Acids

140.91

Weight (kDa)

7.77

Isoelectric Point (pI)

46.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_15	PF13175	10 - 364	5.5e-16	AAA ATPase domain
SMC_N	PF02463	11 - 1200	2.2e-38	RecF/RecN/SMC N terminal domain
AAA_23	PF13476	13 - 220	4.2e-15	AAA domain
AAA_29	PF13555	13 - 56	1.1e-07	P-loop containing region of AAA domain
AAA_21	PF13304	34 - 66	6.8e-08	AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge	PF06470	518 - 632	1.4e-26	SMC proteins Flexible Hinge Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006326)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54670
fragaria_vesca	FvH4_2g21371 FvH4_2g21371 FvH4_2g21371 FvH4_2g21371 FvH4_2g21371 FvH4_2g21371
malus_domestica	MD05G1196500.v1.1 MD10G1183700.v1.1
prunus_persica	Prupe.8G222900_v2.0.a1 Prupe.8G223100_v2.0.a1
pyrus_communis	pycom05g18350 pycom05g18360 pycom10g15870 pycom10g15880
rosa_chinensis	RchiOBHm_Chr6g0287971
rosa_laevigata	RLG00000012450
rosa_multiflora	Rmu_co8056790.1_g000001 Rmu_co8420611.1_g000001 Rmu_sc0005266.1_g000007
rosa_roxburghii	Rroxscaffold_7G00180620 Rroxscaffold_7G00180920
rosa_rugosa	Rorug06G0193700
rosa_samantha	Rh6AG303100 Rh6BG309300 Rh6DG301800
rosa_wichuraiana	Rw6G026210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	494
Acc36I	ACCTGC	1 cut(s)	809
AccB7I	CCANNNNNTGG	1 cut(s)	3221
AccBSI	CCGCTC	2 cut(s)	1908, 2215
AccI	GTMKAC	2 cut(s)	2560, 3281
AccII	CGCG	1 cut(s)	1451
AclWI	GGATC	4 cut(s)	216, 1814, 2089, 3251
AcoI	YGGCCR	2 cut(s)	1586, 3072
AcsI	RAATTY	7 cut(s)	52, 367, 909, 1774, 2948, 3001, 3458
AcuI	CTGAAG	5 cut(s)	585, 644, 707, 1718, 2445
AcvI	CACGTG	1 cut(s)	3491
AdeI	CACNNNGTG	1 cut(s)	3221
AfaI	GTAC	8 cut(s)	178, 344, 380, 1222, 1691, 1743, 1978, 3619
AfeI	AGCGCT	3 cut(s)	328, 562, 3012
AfiI	CCNNNNNNNGG	4 cut(s)	24, 2497, 3025, 3221
AflII	CTTAAG	2 cut(s)	203, 521
AflIII	ACRYGT	2 cut(s)	1744, 2380
AgeI	ACCGGT	1 cut(s)	179
AhlI	ACTAGT	1 cut(s)	1367
AjiI	CACGTC	1 cut(s)	985
AjnI	CCWGG	4 cut(s)	1415, 2679, 3057, 3074
Alw21I	GWGCWC	3 cut(s)	1197, 1393, 2811
Alw26I	GTCTC	6 cut(s)	962, 1084, 1539, 2582, 2911, 3588
Alw44I	GTGCAC	1 cut(s)	1389
AlwI	GGATC	4 cut(s)	216, 1814, 2089, 3251
Ama87I	CYCGRG	1 cut(s)	494
Aor51HI	AGCGCT	3 cut(s)	328, 562, 3012
AoxI	GGCC	7 cut(s)	555, 1422, 1586, 2867, 3072, 3351, 3386
ApaLI	GTGCAC	1 cut(s)	1389
ApeKI	GCWGC	6 cut(s)	37, 575, 664, 1101, 2767, 3422
ApoI	RAATTY	7 cut(s)	52, 367, 909, 1774, 2948, 3001, 3458
AsiGI	ACCGGT	1 cut(s)	179
Asp700I	GAANNNNTTC	1 cut(s)	3521
AspLEI	GCGC	4 cut(s)	329, 563, 3013, 3427
AspS9I	GGNCC	4 cut(s)	86, 116, 2332, 2867
AsuHPI	GGTGA	2 cut(s)	455, 3384
AsuII	TTCGAA	4 cut(s)	464, 2058, 2512, 2747
AsuNHI	GCTAGC	1 cut(s)	3164
AvaI	CYCGRG	1 cut(s)	494
AvaII	GGWCC	3 cut(s)	86, 116, 2332
AxyI	CCTNAGG	1 cut(s)	3635
BaeGI	GKGCMC	1 cut(s)	1393
BanII	GRGCYC	1 cut(s)	2811
BauI	CACGAG	2 cut(s)	1196, 2928
BbrPI	CACGTG	1 cut(s)	3491
BbsI	GAAGAC	4 cut(s)	1445, 1467, 2517, 2573
Bbv12I	GWGCWC	3 cut(s)	1197, 1393, 2811
BbvI	GCAGC	6 cut(s)	49, 587, 651, 1113, 2779, 3409
BccI	CCATC	8 cut(s)	28, 40, 277, 284, 1931, 2858, 2929, 3506
BceAI	ACGGC	3 cut(s)	1232, 1497, 3009
BciT130I	CCWGG	4 cut(s)	1417, 2681, 3059, 3076
BciVI	GTATCC	1 cut(s)	399
BcoDI	GTCTC	6 cut(s)	962, 1084, 1539, 2582, 2911, 3588
BcuI	ACTAGT	1 cut(s)	1367
BfaI	CTAG	8 cut(s)	324, 542, 1205, 1368, 1443, 2069, 3165, 3242
BfmI	CTRYAG	2 cut(s)	665, 3382
BfoI	RGCGCY	3 cut(s)	330, 564, 3014
BfrI	CTTAAG	2 cut(s)	203, 521
BfuAI	ACCTGC	1 cut(s)	809
BfuI	GTATCC	1 cut(s)	399
BglI	GCCNNNNNGGC	1 cut(s)	1421
BglII	AGATCT	2 cut(s)	30, 3180
BisI	GCNGC	9 cut(s)	38, 576, 665, 1102, 2768, 3072, 3351, 3423, 3486
BlsI	GCNGC	9 cut(s)	39, 577, 666, 1103, 2769, 3073, 3352, 3424, 3487
BmcAI	AGTACT	1 cut(s)	1691
Bme1390I	CCNGG	4 cut(s)	1417, 2681, 3059, 3076
Bme18I	GGWCC	3 cut(s)	86, 116, 2332
BmeT110I	CYCGRG	1 cut(s)	494
BmgBI	CACGTC	1 cut(s)	985
BmgT120I	GGNCC	4 cut(s)	86, 116, 2332, 2867
BmiI	GGNNCC	4 cut(s)	118, 511, 2370, 2997
BmrFI	CCNGG	4 cut(s)	1417, 2681, 3059, 3076
BmrI	ACTGGG	1 cut(s)	3227
BmsI	GCATC	8 cut(s)	664, 1026, 1633, 1692, 2830, 3091, 3409, 3493
BmtI	GCTAGC	1 cut(s)	3168
BmuI	ACTGGG	1 cut(s)	3227
BpiI	GAAGAC	4 cut(s)	1445, 1467, 2517, 2573
BplI	GAGNNNNNCTC	4 cut(s)	878, 910, 2889, 2921
BpmI	CTGGAG	2 cut(s)	1924, 3351
Bpu10I	CCTNAGC	1 cut(s)	918
Bpu14I	TTCGAA	4 cut(s)	464, 2058, 2512, 2747
BpuEI	CTTGAG	7 cut(s)	62, 1523, 2222, 2387, 2688, 3530, 3598
BsaAI	YACGTR	5 cut(s)	1388, 1408, 1745, 2381, 3491
BsaI	GGTCTC	2 cut(s)	962, 2911
BsaJI	CCNNGG	6 cut(s)	166, 513, 1416, 1626, 1890, 3354
BsaWI	WCCGGW	1 cut(s)	179
Bsc4I	CCNNNNNNNGG	4 cut(s)	24, 2497, 3025, 3221
Bse118I	RCCGGY	1 cut(s)	179
Bse1I	ACTGG	7 cut(s)	490, 1465, 1813, 1975, 2356, 3019, 3222
Bse21I	CCTNAGG	1 cut(s)	3635
Bse3DI	GCAATG	1 cut(s)	3159
BseBI	CCWGG	4 cut(s)	1417, 2681, 3059, 3076
BseDI	CCNNGG	6 cut(s)	166, 513, 1416, 1626, 1890, 3354
BseLI	CCNNNNNNNGG	4 cut(s)	24, 2497, 3025, 3221
BseMI	GCAATG	1 cut(s)	3159
BseMII	CTCAG	9 cut(s)	212, 639, 876, 909, 917, 2262, 2411, 2432, 2787
BseNI	ACTGG	7 cut(s)	490, 1465, 1813, 1975, 2356, 3019, 3222
BseRI	GAGGAG	9 cut(s)	482, 512, 530, 953, 2225, 2675, 3098, 3348, 3619
BseSI	GKGCMC	1 cut(s)	1393
BseXI	GCAGC	6 cut(s)	49, 587, 651, 1113, 2779, 3409
BseYI	CCCAGC	1 cut(s)	2890
Bsh1236I	CGCG	1 cut(s)	1451
BshFI	GGCC	7 cut(s)	557, 1424, 1588, 2869, 3074, 3353, 3388
BshTI	ACCGGT	1 cut(s)	179
BsiHKAI	GWGCWC	3 cut(s)	1197, 1393, 2811
BsiHKCI	CYCGRG	1 cut(s)	494
BsiSI	CCGG	1 cut(s)	180
BslFI	GGGAC	2 cut(s)	102, 3238
BslI	CCNNNNNNNGG	4 cut(s)	24, 2497, 3025, 3221
BsmAI	GTCTC	6 cut(s)	962, 1084, 1539, 2582, 2911, 3588
BsmFI	GGGAC	2 cut(s)	102, 3238
BsmI	GAATGC	1 cut(s)	1015
BsnI	GGCC	7 cut(s)	557, 1424, 1588, 2869, 3074, 3353, 3388
Bso31I	GGTCTC	2 cut(s)	962, 2911
BsoBI	CYCGRG	1 cut(s)	494
Bsp119I	TTCGAA	4 cut(s)	464, 2058, 2512, 2747
Bsp1286I	GDGCHC	3 cut(s)	1197, 1393, 2811
Bsp19I	CCATGG	1 cut(s)	1626
BspANI	GGCC	7 cut(s)	557, 1424, 1588, 2869, 3074, 3353, 3388
BspCNI	CTCAG	9 cut(s)	211, 640, 877, 910, 918, 2263, 2410, 2433, 2788
BspFNI	CGCG	1 cut(s)	1451
BspHI	TCATGA	1 cut(s)	786
BspLI	GGNNCC	4 cut(s)	118, 511, 2370, 2997
BspMAI	CTGCAG	1 cut(s)	669
BspMI	ACCTGC	1 cut(s)	809
BspOI	GCTAGC	1 cut(s)	3168
BspPI	GGATC	4 cut(s)	216, 1814, 2089, 3251
BspQI	GCTCTTC	1 cut(s)	3371
BspT104I	TTCGAA	4 cut(s)	464, 2058, 2512, 2747
BspTI	CTTAAG	2 cut(s)	203, 521
BspTNI	GGTCTC	2 cut(s)	962, 2911
BsrBI	CCGCTC	2 cut(s)	1908, 2215
BsrDI	GCAATG	1 cut(s)	3159
BsrFI	RCCGGY	1 cut(s)	179
BsrI	ACTGG	7 cut(s)	490, 1465, 1813, 1975, 2356, 3019, 3222
BssAI	RCCGGY	1 cut(s)	179
BssECI	CCNNGG	6 cut(s)	166, 513, 1416, 1626, 1890, 3354
BssNAI	GTATAC	2 cut(s)	2561, 3282
BssSI	CACGAG	2 cut(s)	1196, 2928
BssT1I	CCWWGG	3 cut(s)	1626, 1890, 3354
Bst1107I	GTATAC	2 cut(s)	2561, 3282
Bst2BI	CACGAG	2 cut(s)	1196, 2928
Bst2UI	CCWGG	4 cut(s)	1417, 2681, 3059, 3076
Bst6I	CTCTTC	6 cut(s)	683, 1082, 1737, 2634, 3119, 3371
BstAFI	CTTAAG	2 cut(s)	203, 521
BstAPI	GCANNNNNTGC	2 cut(s)	3452, 3477
BstBAI	YACGTR	5 cut(s)	1388, 1408, 1745, 2381, 3491
BstBI	TTCGAA	4 cut(s)	464, 2058, 2512, 2747
BstC8I	GCNNGC	7 cut(s)	6, 559, 840, 949, 2454, 2697, 3166
BstDSI	CCRYGG	1 cut(s)	1626
BstFNI	CGCG	1 cut(s)	1451
BstH2I	RGCGCY	3 cut(s)	330, 564, 3014
BstHHI	GCGC	4 cut(s)	329, 563, 3013, 3427
BstMAI	GTCTC	6 cut(s)	962, 1084, 1539, 2582, 2911, 3588
BstMWI	GCNNNNNNNGC	9 cut(s)	661, 1402, 1421, 2212, 2705, 3356, 3452, 3477, 3528
BstNI	CCWGG	4 cut(s)	1417, 2681, 3059, 3076
BstSCI	CCNGG	4 cut(s)	1415, 2679, 3057, 3074
BstSFI	CTRYAG	2 cut(s)	665, 3382
BstSLI	GKGCMC	1 cut(s)	1393
BstUI	CGCG	1 cut(s)	1451
BstV1I	GCAGC	6 cut(s)	49, 587, 651, 1113, 2779, 3409
BstV2I	GAAGAC	4 cut(s)	1445, 1467, 2517, 2573
BstX2I	RGATCY	4 cut(s)	30, 208, 2081, 3180
BstXI	CCANNNNNNTGG	1 cut(s)	2363
BstYI	RGATCY	4 cut(s)	30, 208, 2081, 3180
BstZ17I	GTATAC	2 cut(s)	2561, 3282
Bsu36I	CCTNAGG	1 cut(s)	3635
BsuI	GTATCC	1 cut(s)	399
BsuRI	GGCC	7 cut(s)	557, 1424, 1588, 2869, 3074, 3353, 3388
BtgI	CCRYGG	1 cut(s)	1626
BtrI	CACGTC	1 cut(s)	985
BtsI	GCAGTG	2 cut(s)	102, 1391
BtsIMutI	CAGTG	4 cut(s)	102, 1391, 1617, 3215
BveI	ACCTGC	1 cut(s)	809
Cac8I	GCNNGC	7 cut(s)	6, 559, 840, 949, 2454, 2697, 3166
CciI	TCATGA	1 cut(s)	786
CfoI	GCGC	4 cut(s)	329, 563, 3013, 3427
Cfr10I	RCCGGY	1 cut(s)	179
Cfr13I	GGNCC	4 cut(s)	86, 116, 2332, 2867
CseI	GACGC	2 cut(s)	158, 1532
Csp6I	GTAC	8 cut(s)	177, 343, 379, 1221, 1690, 1742, 1977, 3618
CspAI	ACCGGT	1 cut(s)	179
CviAII	CATG	6 cut(s)	787, 1562, 1627, 3269, 3316, 3477
CviQI	GTAC	8 cut(s)	177, 343, 379, 1221, 1690, 1742, 1977, 3618
DraI	TTTAAA	2 cut(s)	1312, 1921
DraIII	CACNNNGTG	1 cut(s)	3221
EaeI	YGGCCR	2 cut(s)	1586, 3072
Eam1104I	CTCTTC	6 cut(s)	683, 1082, 1737, 2634, 3119, 3371
EarI	CTCTTC	6 cut(s)	683, 1082, 1737, 2634, 3119, 3371
EciI	GGCGGA	1 cut(s)	3584
Ecl136II	GAGCTC	1 cut(s)	2809
Eco130I	CCWWGG	3 cut(s)	1626, 1890, 3354
Eco147I	AGGCCT	2 cut(s)	1424, 3388
Eco24I	GRGCYC	1 cut(s)	2811
Eco31I	GGTCTC	2 cut(s)	962, 2911
Eco32I	GATATC	1 cut(s)	733
Eco47I	GGWCC	3 cut(s)	86, 116, 2332
Eco47III	AGCGCT	3 cut(s)	328, 562, 3012
Eco53kI	GAGCTC	1 cut(s)	2809
Eco57I	CTGAAG	5 cut(s)	585, 644, 707, 1718, 2445
Eco72I	CACGTG	1 cut(s)	3491
Eco81I	CCTNAGG	1 cut(s)	3635
Eco88I	CYCGRG	1 cut(s)	494
EcoICRI	GAGCTC	1 cut(s)	2809
EcoRII	CCWGG	4 cut(s)	1415, 2679, 3057, 3074
EcoRV	GATATC	1 cut(s)	733
EcoT14I	CCWWGG	3 cut(s)	1626, 1890, 3354
EcoT38I	GRGCYC	1 cut(s)	2811
ErhI	CCWWGG	3 cut(s)	1626, 1890, 3354
FaeI	CATG	6 cut(s)	790, 1565, 1630, 3272, 3319, 3480
FalI	AAGNNNNNCTT	6 cut(s)	564, 596, 2175, 2207, 3539, 3571
FaqI	GGGAC	2 cut(s)	102, 3238
FatI	CATG	6 cut(s)	786, 1561, 1626, 3268, 3315, 3476
FauI	CCCGC	1 cut(s)	1951
FblI	GTMKAC	2 cut(s)	2560, 3281
Fnu4HI	GCNGC	9 cut(s)	38, 576, 665, 1102, 2768, 3072, 3351, 3423, 3486
FriOI	GRGCYC	1 cut(s)	2811
Fsp4HI	GCNGC	9 cut(s)	38, 576, 665, 1102, 2768, 3072, 3351, 3423, 3486
FspBI	CTAG	8 cut(s)	324, 542, 1205, 1368, 1443, 2069, 3165, 3242
GlaI	GCGC	4 cut(s)	328, 562, 3012, 3426
GluI	GCNGC	9 cut(s)	38, 576, 665, 1102, 2768, 3072, 3351, 3423, 3486
GsaI	CCCAGC	1 cut(s)	2894
GsuI	CTGGAG	2 cut(s)	1924, 3351
HaeII	RGCGCY	3 cut(s)	330, 564, 3014
HaeIII	GGCC	7 cut(s)	557, 1424, 1588, 2869, 3074, 3353, 3388
HapII	CCGG	1 cut(s)	180
HgaI	GACGC	2 cut(s)	158, 1532
HhaI	GCGC	4 cut(s)	329, 563, 3013, 3427
Hin1II	CATG	6 cut(s)	790, 1565, 1630, 3272, 3319, 3480
Hin6I	GCGC	4 cut(s)	327, 561, 3011, 3425
HinP1I	GCGC	4 cut(s)	327, 561, 3011, 3425
HincII	GTYRAC	2 cut(s)	185, 1130
HindII	GTYRAC	2 cut(s)	185, 1130
HindIII	AAGCTT	3 cut(s)	258, 899, 3147
HpaII	CCGG	1 cut(s)	180
HphI	GGTGA	2 cut(s)	455, 3384
Hpy166II	GTNNAC	8 cut(s)	185, 1130, 1391, 2135, 2332, 2561, 3282, 3589
Hpy8I	GTNNAC	8 cut(s)	185, 1130, 1391, 2135, 2332, 2561, 3282, 3589
Hpy99I	CGWCG	2 cut(s)	1348, 1877
HpyF10VI	GCNNNNNNNGC	9 cut(s)	661, 1402, 1421, 2212, 2705, 3356, 3452, 3477, 3528
Hsp92II	CATG	6 cut(s)	790, 1565, 1630, 3272, 3319, 3480
HspAI	GCGC	4 cut(s)	327, 561, 3011, 3425
LguI	GCTCTTC	1 cut(s)	3371
LmnI	GCTCC	5 cut(s)	517, 1054, 1905, 2212, 3001
Lsp1109I	GCAGC	6 cut(s)	49, 587, 651, 1113, 2779, 3409
LweI	GCATC	8 cut(s)	664, 1026, 1633, 1692, 2830, 3091, 3409, 3493
MaeI	CTAG	8 cut(s)	324, 542, 1205, 1368, 1443, 2069, 3165, 3242
MaeIII	GTNAC	8 cut(s)	233, 1383, 1615, 1927, 2444, 2502, 3311, 3491
MbiI	CCGCTC	2 cut(s)	1908, 2215
MfeI	CAATTG	3 cut(s)	77, 603, 2620
MflI	RGATCY	4 cut(s)	30, 208, 2081, 3180
MhlI	GDGCHC	3 cut(s)	1197, 1393, 2811
MlyI	GAGTC	5 cut(s)	264, 483, 1541, 2117, 2531
MmeI	TCCRAC	5 cut(s)	1547, 1834, 2286, 2637, 3562
MroXI	GAANNNNTTC	1 cut(s)	3521
MslI	CAYNNNNRTG	1 cut(s)	1410
MspA1I	CMGCKG	4 cut(s)	1104, 2473, 2594, 2770
MspCI	CTTAAG	2 cut(s)	203, 521
MspI	CCGG	1 cut(s)	180
MspR9I	CCNGG	4 cut(s)	1417, 2681, 3059, 3076
MunI	CAATTG	3 cut(s)	77, 603, 2620
Mva1269I	GAATGC	1 cut(s)	1015
MvaI	CCWGG	4 cut(s)	1417, 2681, 3059, 3076
MvnI	CGCG	1 cut(s)	1451
MwoI	GCNNNNNNNGC	9 cut(s)	661, 1402, 1421, 2212, 2705, 3356, 3452, 3477, 3528
NcoI	CCATGG	1 cut(s)	1626
NheI	GCTAGC	1 cut(s)	3164
NlaIII	CATG	6 cut(s)	790, 1565, 1630, 3272, 3319, 3480
NlaIV	GGNNCC	4 cut(s)	118, 511, 2370, 2997
NmeAIII	GCCGAG	1 cut(s)	147
NmuCI	GTSAC	6 cut(s)	233, 1615, 1927, 2502, 3311, 3491
NspV	TTCGAA	4 cut(s)	464, 2058, 2512, 2747
PaeR7I	CTCGAG	1 cut(s)	494
PagI	TCATGA	1 cut(s)	786
PceI	AGGCCT	2 cut(s)	1424, 3388
PciSI	GCTCTTC	1 cut(s)	3371
PcsI	WCGNNNNNNNCGW	2 cut(s)	168, 310
PctI	GAATGC	1 cut(s)	1015
PdmI	GAANNNNTTC	1 cut(s)	3521
PfeI	GAWTC	9 cut(s)	452, 469, 758, 1446, 2149, 2509, 2858, 2966, 3650
PflFI	GACNNNGTC	1 cut(s)	2572
PflMI	CCANNNNNTGG	1 cut(s)	3221
PfoI	TCCNGGA	1 cut(s)	2679
PinAI	ACCGGT	1 cut(s)	179
PkrI	GCNGC	9 cut(s)	39, 577, 666, 1103, 2769, 3073, 3352, 3424, 3487
PleI	GAGTC	5 cut(s)	264, 483, 1540, 2116, 2530
PmaCI	CACGTG	1 cut(s)	3491
PmlI	CACGTG	1 cut(s)	3491
PpsI	GAGTC	5 cut(s)	264, 483, 1540, 2116, 2530
Ppu21I	YACGTR	5 cut(s)	1388, 1408, 1745, 2381, 3491
Psp124BI	GAGCTC	1 cut(s)	2811
Psp6I	CCWGG	4 cut(s)	1415, 2679, 3057, 3074
PspCI	CACGTG	1 cut(s)	3491
PspFI	CCCAGC	1 cut(s)	2890
PspGI	CCWGG	4 cut(s)	1415, 2679, 3057, 3074
PspN4I	GGNNCC	4 cut(s)	118, 511, 2370, 2997
PspPI	GGNCC	4 cut(s)	86, 116, 2332, 2867
PspXI	VCTCGAGB	1 cut(s)	494
PstI	CTGCAG	1 cut(s)	669
PsuI	RGATCY	4 cut(s)	30, 208, 2081, 3180
PsyI	GACNNNGTC	1 cut(s)	2572
PvuII	CAGCTG	2 cut(s)	2473, 2770
RsaI	GTAC	8 cut(s)	178, 344, 380, 1222, 1691, 1743, 1978, 3619
RsaNI	GTAC	8 cut(s)	177, 343, 379, 1221, 1690, 1742, 1977, 3618
RseI	CAYNNNNRTG	1 cut(s)	1410
SacI	GAGCTC	1 cut(s)	2811
SapI	GCTCTTC	1 cut(s)	3371
SatI	GCNGC	9 cut(s)	38, 576, 665, 1102, 2768, 3072, 3351, 3423, 3486
Sau96I	GGNCC	4 cut(s)	86, 116, 2332, 2867
ScaI	AGTACT	1 cut(s)	1691
SchI	GAGTC	5 cut(s)	264, 483, 1541, 2117, 2531
ScrFI	CCNGG	4 cut(s)	1417, 2681, 3059, 3076
SduI	GDGCHC	3 cut(s)	1197, 1393, 2811
SfaNI	GCATC	8 cut(s)	664, 1026, 1633, 1692, 2830, 3091, 3409, 3493
SfcI	CTRYAG	2 cut(s)	665, 3382
Sfr274I	CTCGAG	1 cut(s)	494
SfuI	TTCGAA	4 cut(s)	464, 2058, 2512, 2747
SinI	GGWCC	3 cut(s)	86, 116, 2332
SlaI	CTCGAG	1 cut(s)	494
SmiMI	CAYNNNNRTG	1 cut(s)	1410
SpeI	ACTAGT	1 cut(s)	1367
SseBI	AGGCCT	2 cut(s)	1424, 3388
SspI	AATATT	1 cut(s)	2077
SspMI	CTAG	8 cut(s)	324, 542, 1205, 1368, 1443, 2069, 3165, 3242
SstI	GAGCTC	1 cut(s)	2811
StuI	AGGCCT	2 cut(s)	1424, 3388
StyD4I	CCNGG	4 cut(s)	1415, 2679, 3057, 3074
StyI	CCWWGG	3 cut(s)	1626, 1890, 3354
TatI	WGTACW	3 cut(s)	378, 1689, 3617
TauI	GCSGC	3 cut(s)	3074, 3353, 3488
TfiI	GAWTC	9 cut(s)	452, 469, 758, 1446, 2149, 2509, 2858, 2966, 3650
TscAI	CASTG	4 cut(s)	109, 1398, 1624, 3222
TseFI	GTSAC	6 cut(s)	233, 1615, 1927, 2502, 3311, 3491
TseI	GCWGC	6 cut(s)	37, 575, 664, 1101, 2767, 3422
Tsp45I	GTSAC	6 cut(s)	233, 1615, 1927, 2502, 3311, 3491
TspGWI	ACGGA	4 cut(s)	1394, 2521, 2929, 3249
TspRI	CASTG	4 cut(s)	109, 1398, 1624, 3222
Tth111I	GACNNNGTC	1 cut(s)	2572
Van91I	CCANNNNNTGG	1 cut(s)	3221
Vha464I	CTTAAG	2 cut(s)	203, 521
VneI	GTGCAC	1 cut(s)	1389
VpaK11BI	GGWCC	3 cut(s)	86, 116, 2332
XapI	RAATTY	7 cut(s)	52, 367, 909, 1774, 2948, 3001, 3458
XbaI	TCTAGA	1 cut(s)	1204
XhoI	CTCGAG	1 cut(s)	494
XmiI	GTMKAC	2 cut(s)	2560, 3281
XmnI	GAANNNNTTC	1 cut(s)	3521
XspI	CTAG	8 cut(s)	324, 542, 1205, 1368, 1443, 2069, 3165, 3242
ZrmI	AGTACT	1 cut(s)	1691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.