Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0006326

Physical Location & Seq

Genomic Coordinates Reverse (-)

18892537 .. 18902005

Gene Structure 9469 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012450

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3510 bp

ATGCCGTCCCTGATCTCCCAGGGCAAAATCCACCGCCTAGAGCTCGAGAATTTCAAGTCTTACCGGGGCCACCAGGTCATCGGCCCCTTCTCCGACTTCACGGCGATTATCGGCCCCAATGGCTCCGGCAAGTCCAACCTCATGGACGCCATAAGCTTCGTCCTTGGGGTCAAGACCGGCCAGCTCCGTGGGTCCCAGCTGAAGGACCTGATCTACGCCATGGACGACAGTGAGAAGAGCGAGAAAGGCCGCAGGGCGTTTGTGAGCTTGGTTTACCAGCTTGCGAATGAGTCTGAGATCCAGTTCACAAGGGCCATCACTAGCTCCGGCGGCAGTGAGTACCGCATAGACGGCAAGTCAGTATCGTGGGAAGCTTATAATGAGAAGCTTAAGTCCCTTGGAATTCTAGTCAAGGCTCGCAATTTTCTTGTCTTTCAGGGTGATGTCGAGTCCATTGCCTCTAAAAATCCTAAGGAACTCACTGGACTGCTTGAGCAGATCTCAGGGTCTGATGAGCTCAAGAGAGACTATGAAAAGTATGAAGAAGAGAAAGGCATAGCTGAAGAAAAAGCAGCCCTTGTTTATCAGAAAAAAAGAACCATAGTAGCGGAGAGGAAGCAAAAGAAAGAGCAAAAGGAAGAAGCTGAGAAGCACATTCGCCTGCAAAATGAACTGAAATCTTTGAAGAGAGAACACTTTTTGTGGCAGTTGCTTAACATAGAAAAAGATATAACGAAAACAACTGATGAGCTTGAAGCTGAAAAGAGAAGCCGCGAACAGGTTATGCAAGAACTAGATGATTTTCAGCAAGAAGCAAACAAAAAGAAGAAAGAACTTGCCAAATACTTAAAAGAGATTGCTCAATGTGAAAAGAAGATTGCTGAGAGAAGTAGTAGACTTGACAGAAGTAAACCAGAGCTGCTGAAACTGAAAGAGGAAATGTCTCGAATAAATTCAAAAATCAAGAAAAGTAAGACGGAGCTTGGTAGAAAAGAACAAGAACGGGGGAGACATAAGGAGGAAATTAAAAAGCTGCAGAAGGGCATCCAGGATTTGACCACACAACTAGAGGATTTACAGGAAAAAGGCCGAGACAGTGGTGAAAAATTACATTTAGACGATACTAAATTAAGGGAATATTTTCAAGTGAAGGAGGATGCTGGGATGAAAACTGCAAAGCTAAAAGATGAGAAAGAGGTTCTGGATCGGCAACAGCATGCTGATCTTGAAGCTCAAAAGAATTTGGAAGAAAATCTTCAACAGTTGAGAAGTAGGGAGCGTGAACTGGATTCACAGAATGAGCAAATGCTGACAAGGCAGAGAAAAATTAAAGAAAACTCTGCAAAACACAAGCAGGAATCAAAAAATCTGAATAATGAATTGCATGTGATGCAGGAGAAACATCAGAATGCCAGACAAAAGTATGAAAATCTGAAGTCAAAAATTGATGAATTAGAAAAGCAATTACGTGAACTGAAGGCCGACAGATATGAAAATGAGAGAGATTCCAGGTTGTCTCAGGCAGTGGAGACTCTCAAGCGCTTGTTTCAGGGTGTCCATGGTCGCATGACTGAACTCTGTAGGCCAACACAAAAGAAGTATAATCTTGCTGTCACTGTTGCCATGGGAAAATTTATGGATGCAGTCGTAGTTGAAGATGAACAAACAGGAAAGGAGTGTATCAAGTATTTGAAAGAACAAAGGCTTCCTCCTCAGACATTCATACCTCTCCAATCTGTTCGTGTAAAGCAAGTAATGGAGAGATTGCGTAACTTGGGTGGTACTGCAAAACTGGTTTTTGATGTTAAGGCTATTCTTTTTGCGGTTGGCAATACACTTGTTTGTGACGAACTTGATGAGGCTAAGCGCCTAAGCTGGAGTGGACTGAAGAAGAAGAAAGAACAGTTTGAGTTGGAGCTAGAAGAGCTTGGGTCAATTAGAGAGATGCAGCTAAAGGAGTCTGAAACAGCTGGCAGAATTAGTGGGCTCGACAAGAAAATCCAATATGCAGATATCGAGAAGGAAAGCATTAAGGACAAACTTGCAAATTTGGTCCGTGAGAGGCAGAATATAAAAGAAGAGATTGATCGTATTAGTCCTGATCTTCTGAAGCTAAAACAAGCTGTAGATAAGCGGAGCACAGAAATCAATAAGCTTGAGAAGAGGATAAATGATATTGTTGACAGACTCTACAAAGGCTTTAGTAAATCTGTTGGGGTAGATAACATTCGCGAGTATGAAGAAAAACAACTCAAGGCTACCCAATCCATGGCTGAAGAGAGGCTTAGTTTGAGTAGCCAGCTATCAAAACTGAAATACCAGCTAGAGTATGAGCAAAATCGGGACATGGATACACGAATTGAAGAACTCCATTCATCTATTAGTAACCTACTAAAAGATTTAGAACGGGTTCAAAAGAAAGAGGCCGAAGCCAACTCTGCAGCAGAAAAAGCCTCTGATGAGATTCTTCGGTTGAAGGAAGAAGCACAAGAATGGAAGTCCAAGTCGGAAGGGTGTGAAAAGGAAATTCAAGAATGGAACAAGCGGGGTTCTACTGCAACATCAAATGTATCCAAACTTAATCGACAGATAAATTCTAAGGAATCGCAGATTGAGCAGCTAACATCTCGAAAGCAGGAGATAATAGAAAATTGTGAACTACAGCAAATAAGCCTTCCGATCATTTCAGATCCAATGGAGACTGAATCCTCCACGACAGGCCCAGTATTTGATTTTGATGAGTTAGATGAGTCTCTATTGAGGAATAGGAGGCCTTCTGAGCGGGAGAAAGTTGAGTTAGACTTTAAGAAACAAATGGATGCCAAGTTATCAGAAATTGAAAGAACAGCTCCCAATTTGAAGGCCATGGACCAATATGAGGCTCTACAAGAAAAGGAAAGAGATGTAACTGGAGAGTTTGAAGTGGCCAGAAAAGAACAGAAACAAAAGGCTGATCTGTTTAACTCGGTTAAGCAAAGGAGGTATGAGTTGTTTATGGATGCCTTCAACCATATTTCCAGTAATATTGATAAGATATACAAACAACTGACGAAGAGCAATACACATCCCCTGGGTGGGACGGCATATTTGAATTTAGAAAATGAAGATGATCCGTTTTTACATGGTGTCAAGTACACTACTATGCCCCCAACGAAGCGCTTTCGAGATATGGAACAATTATCTGGCGGAGAGAAAACTGTTGCAGCGTTAGCATTACTGTTTTCCATACACAGCTATAGGCCTTCGCCATTTTTCATACTGGATGAAGTTGATGCTGCACTGGATAACTTAAATGTAGCAAAGGTTGCTCGATTCATCCGTTCAAAGTCATGCCAAGGAGCCAGGGTTAATCAGGATACTGAAGGAGGCAATGGTTTTCAAAGTATTGTAATATCTCTAAAAGATAGCTTCTACGACAAGGCTGAAGCTCTGGTTGGGGTTTTCAGGGACGCTGAAATGAGTTGTTCCAAAACACTCTCATTTGACTTGACTAAATTTCGGGAGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000793 GO:0000819 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0008150 GO:0008278 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0022402 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044444 GO:0044446 GO:0044464 GO:0051276 GO:0071840 GO:0098813

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1170

Amino Acids

135.27

Weight (kDa)

7.79

Isoelectric Point (pI)

44.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_15	PF13175	9 - 357	2.4e-12	AAA ATPase domain
SMC_N	PF02463	10 - 1150	7.7e-43	RecF/RecN/SMC N terminal domain
AAA_29	PF13555	11 - 55	3.7e-08	P-loop containing region of AAA domain
AAA_23	PF13476	12 - 219	4.3e-16	AAA domain
AAA_21	PF13304	33 - 61	1.4e-07	AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge	PF06470	517 - 624	3.3e-20	SMC proteins Flexible Hinge Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006326)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54670
fragaria_vesca	FvH4_2g21371 FvH4_2g21371 FvH4_2g21371 FvH4_2g21371 FvH4_2g21371 FvH4_2g21371
malus_domestica	MD05G1196500.v1.1 MD10G1183700.v1.1
prunus_persica	Prupe.8G222900_v2.0.a1 Prupe.8G223100_v2.0.a1
pyrus_communis	pycom05g18350 pycom05g18360 pycom10g15870 pycom10g15880
rosa_chinensis	RchiOBHm_Chr6g0287971
rosa_laevigata	RLG00000012450
rosa_multiflora	Rmu_co8056790.1_g000001 Rmu_co8420611.1_g000001 Rmu_sc0005266.1_g000007
rosa_roxburghii	Rroxscaffold_7G00180620 Rroxscaffold_7G00180920
rosa_rugosa	Rorug06G0193700
rosa_samantha	Rh6AG303100 Rh6BG309300 Rh6DG301800
rosa_wichuraiana	Rw6G026210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	378
AccB7I	CCANNNNNTGG	1 cut(s)	2269
AccBSI	CCGCTC	1 cut(s)	2782
AccI	GTMKAC	1 cut(s)	895
AccII	CGCG	2 cut(s)	774, 2232
AclWI	GGATC	4 cut(s)	292, 1212, 2684, 3104
AcoI	YGGCCR	2 cut(s)	178, 2925
AcuI	CTGAAG	9 cut(s)	221, 582, 1454, 1496, 1907, 2129, 2295, 3381, 3444
AcyI	GRCGYC	1 cut(s)	147
AfaI	GTAC	3 cut(s)	341, 1783, 3134
AfeI	AGCGCT	2 cut(s)	1541, 3158
AfiI	CCNNNNNNNGG	6 cut(s)	202, 778, 2269, 2878, 3074, 3075
AflII	CTTAAG	1 cut(s)	389
AhdI	GACNNNNNGTC	1 cut(s)	355
AjnI	CCWGG	6 cut(s)	18, 72, 1047, 1508, 3069, 3341
AjuI	GAANNNNNNNTTGG	2 cut(s)	3417, 3449
Alw21I	GWGCWC	3 cut(s)	45, 519, 2141
Alw26I	GTCTC	8 cut(s)	519, 948, 1003, 1086, 1521, 1523, 2693, 2757
AlwI	GGATC	4 cut(s)	292, 1212, 2684, 3104
AlwNI	CAGNNNCTG	1 cut(s)	509
Ama87I	CYCGRG	1 cut(s)	44
Aor51HI	AGCGCT	2 cut(s)	1541, 3158
ApeKI	GCWGC	8 cut(s)	572, 919, 1033, 1948, 2441, 2617, 3203, 3275
ArsI	GACNNNNNNTTYG	2 cut(s)	3070, 3102
Asp700I	GAANNNNTTC	4 cut(s)	952, 1140, 1254, 2409
AspLEI	GCGC	3 cut(s)	1542, 1869, 3159
AspS9I	GGNCC	9 cut(s)	67, 83, 113, 192, 205, 312, 2053, 2720, 2869
AsuC2I	CCSGG	1 cut(s)	65
AsuHPI	GGTGA	2 cut(s)	452, 1112
AvaI	CYCGRG	1 cut(s)	44
AvaII	GGWCC	4 cut(s)	192, 205, 2053, 2869
AxyI	CCTNAGG	1 cut(s)	471
BalI	TGGCCA	1 cut(s)	2927
BanII	GRGCYC	3 cut(s)	45, 519, 1989
Bbv12I	GWGCWC	3 cut(s)	45, 519, 2141
BbvI	GCAGC	8 cut(s)	584, 906, 1020, 1960, 2453, 2629, 3215, 3262
BccI	CCATC	1 cut(s)	323
BceAI	ACGGC	3 cut(s)	117, 367, 3096
BcgI	CGANNNNNNTGC	2 cut(s)	437, 471
BciT130I	CCWGG	6 cut(s)	20, 74, 1049, 1510, 3071, 3343
BciVI	GTATCC	3 cut(s)	2344, 2581, 3349
BcnI	CCSGG	1 cut(s)	65
BcoDI	GTCTC	8 cut(s)	519, 948, 1003, 1086, 1521, 1523, 2693, 2757
BfaI	CTAG	7 cut(s)	38, 321, 407, 794, 1067, 1919, 2324
BfmI	CTRYAG	6 cut(s)	1034, 1579, 2124, 2439, 2660, 3235
BfoI	RGCGCY	3 cut(s)	1543, 1870, 3160
BfrI	CTTAAG	1 cut(s)	389
BfuI	GTATCC	3 cut(s)	2344, 2581, 3349
BglI	GCCNNNNNGGC	1 cut(s)	120
BglII	AGATCT	1 cut(s)	498
BlpI	GCTNAGC	1 cut(s)	1863
Bme1390I	CCNGG	7 cut(s)	20, 65, 74, 1049, 1510, 3071, 3343
Bme18I	GGWCC	4 cut(s)	192, 205, 2053, 2869
BmeRI	GACNNNNNGTC	1 cut(s)	355
BmeT110I	CYCGRG	1 cut(s)	44
BmgT120I	GGNCC	9 cut(s)	67, 83, 113, 192, 205, 312, 2053, 2720, 2869
BmiI	GGNNCC	7 cut(s)	68, 85, 115, 124, 193, 194, 3340
BmrFI	CCNGG	7 cut(s)	20, 65, 74, 1049, 1510, 3071, 3343
BmrI	ACTGGG	1 cut(s)	2717
BmsI	GCATC	8 cut(s)	1053, 1147, 1380, 1630, 1935, 2809, 2989, 3262
BmuI	ACTGGG	1 cut(s)	2717
BplI	GAGNNNNNCTC	2 cut(s)	485, 517
BpmI	CTGGAG	2 cut(s)	1897, 2931
Bpu10I	CCTNAGC	1 cut(s)	1871
Bpu1102I	GCTNAGC	1 cut(s)	1863
BpuEI	CTTGAG	5 cut(s)	503, 512, 1520, 2177, 2237
BpuMI	CCSGG	1 cut(s)	65
BsaAI	YACGTR	1 cut(s)	1469
BsaHI	GRCGYC	1 cut(s)	147
BsaXI	ACNNNNNCTCC	2 cut(s)	3330, 3360
Bsc4I	CCNNNNNNNGG	6 cut(s)	202, 778, 2269, 2878, 3074, 3075
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	9 cut(s)	301, 487, 1290, 1797, 2723, 2914, 3018, 3264, 3285
Bse21I	CCTNAGG	1 cut(s)	471
Bse3DI	GCAATG	2 cut(s)	453, 3376
BseBI	CCWGG	6 cut(s)	20, 74, 1049, 1510, 3071, 3343
BseGI	GGATG	9 cut(s)	1044, 1162, 1170, 1645, 2824, 3004, 3064, 3268, 3315
BseLI	CCNNNNNNNGG	6 cut(s)	202, 778, 2269, 2878, 3074, 3075
BseMI	GCAATG	2 cut(s)	453, 3376
BseMII	CTCAG	7 cut(s)	285, 516, 636, 873, 1532, 1727, 2769
BseNI	ACTGG	9 cut(s)	301, 487, 1290, 1797, 2723, 2914, 3018, 3264, 3285
BseRI	GAGGAG	1 cut(s)	1701
BseXI	GCAGC	8 cut(s)	584, 906, 1020, 1960, 2453, 2629, 3215, 3262
BseYI	CCCAGC	2 cut(s)	195, 1160
BsgI	GTGCAG	1 cut(s)	3261
Bsh1236I	CGCG	2 cut(s)	774, 2232
BsiHKAI	GWGCWC	3 cut(s)	45, 519, 2141
BsiHKCI	CYCGRG	1 cut(s)	44
BsiSI	CCGG	4 cut(s)	64, 126, 177, 327
BslFI	GGGAC	5 cut(s)	178, 379, 2357, 3091, 3461
BslI	CCNNNNNNNGG	6 cut(s)	202, 778, 2269, 2878, 3074, 3075
BsmAI	GTCTC	8 cut(s)	519, 948, 1003, 1086, 1521, 1523, 2693, 2757
BsmFI	GGGAC	5 cut(s)	178, 379, 2357, 3091, 3461
BsmI	GAATGC	1 cut(s)	1414
BsoBI	CYCGRG	1 cut(s)	44
Bsp1286I	GDGCHC	4 cut(s)	45, 519, 1989, 2141
Bsp1720I	GCTNAGC	1 cut(s)	1863
Bsp19I	CCATGG	5 cut(s)	219, 1558, 1623, 2268, 2865
Bsp68I	TCGCGA	1 cut(s)	2232
BspCNI	CTCAG	7 cut(s)	286, 515, 637, 874, 1531, 1726, 2770
BspFNI	CGCG	2 cut(s)	774, 2232
BspLI	GGNNCC	7 cut(s)	68, 85, 115, 124, 193, 194, 3340
BspMAI	CTGCAG	2 cut(s)	1038, 2443
BspPI	GGATC	4 cut(s)	292, 1212, 2684, 3104
BspQI	GCTCTTC	3 cut(s)	230, 1917, 3047
BspTI	CTTAAG	1 cut(s)	389
BsrBI	CCGCTC	1 cut(s)	2782
BsrDI	GCAATG	2 cut(s)	453, 3376
BsrFI	RCCGGY	1 cut(s)	176
BsrI	ACTGG	9 cut(s)	301, 487, 1290, 1797, 2723, 2914, 3018, 3264, 3285
BssAI	RCCGGY	1 cut(s)	176
BssNI	GRCGYC	1 cut(s)	147
BssT1I	CCWWGG	8 cut(s)	163, 219, 397, 1558, 1623, 2268, 2865, 3334
Bst2UI	CCWGG	6 cut(s)	20, 74, 1049, 1510, 3071, 3343
Bst4CI	ACNGT	7 cut(s)	230, 1097, 1263, 1618, 1905, 3199, 3219
Bst6I	CTCTTC	8 cut(s)	230, 540, 680, 1917, 2073, 2156, 2271, 3047
BstACI	GRCGYC	1 cut(s)	147
BstAFI	CTTAAG	1 cut(s)	389
BstAPI	GCANNNNNTGC	2 cut(s)	1390, 3305
BstBAI	YACGTR	1 cut(s)	1469
BstC8I	GCNNGC	7 cut(s)	182, 282, 418, 662, 1218, 1972, 2300
BstDSI	CCRYGG	6 cut(s)	187, 219, 1558, 1623, 2268, 2865
BstF5I	GGATG	9 cut(s)	1044, 1162, 1170, 1645, 2824, 3004, 3064, 3268, 3315
BstFNI	CGCG	2 cut(s)	774, 2232
BstH2I	RGCGCY	3 cut(s)	1543, 1870, 3160
BstHHI	GCGC	3 cut(s)	1542, 1869, 3159
BstMAI	GTCTC	8 cut(s)	519, 948, 1003, 1086, 1521, 1523, 2693, 2757
BstNI	CCWGG	6 cut(s)	20, 74, 1049, 1510, 3071, 3343
BstNSI	RCATGY	2 cut(s)	1220, 1388
BstSCI	CCNGG	7 cut(s)	18, 63, 72, 1047, 1508, 3069, 3341
BstSFI	CTRYAG	6 cut(s)	1034, 1579, 2124, 2439, 2660, 3235
BstUI	CGCG	2 cut(s)	774, 2232
BstV1I	GCAGC	8 cut(s)	584, 906, 1020, 1960, 2453, 2629, 3215, 3262
BstX2I	RGATCY	3 cut(s)	297, 498, 2689
BstXI	CCANNNNNNTGG	2 cut(s)	142, 188
BstYI	RGATCY	3 cut(s)	297, 498, 2689
Bsu36I	CCTNAGG	1 cut(s)	471
BsuI	GTATCC	3 cut(s)	2344, 2581, 3349
BtgI	CCRYGG	6 cut(s)	187, 219, 1558, 1623, 2268, 2865
BtsCI	GGATG	9 cut(s)	1044, 1162, 1170, 1645, 2824, 3004, 3064, 3268, 3315
BtsI	GCAGTG	2 cut(s)	340, 1530
BtsIMutI	CAGTG	7 cut(s)	235, 340, 480, 1102, 1530, 1614, 3278
BtuMI	TCGCGA	1 cut(s)	2232
Cac8I	GCNNGC	7 cut(s)	182, 282, 418, 662, 1218, 1972, 2300
CaiI	CAGNNNCTG	1 cut(s)	509
CfoI	GCGC	3 cut(s)	1542, 1869, 3159
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	9 cut(s)	67, 83, 113, 192, 205, 312, 2053, 2720, 2869
CseI	GACGC	2 cut(s)	155, 3458
CsiI	ACCWGGT	1 cut(s)	72
Csp6I	GTAC	3 cut(s)	340, 1782, 3133
CviQI	GTAC	3 cut(s)	340, 1782, 3133
DriI	GACNNNNNGTC	1 cut(s)	355
EaeI	YGGCCR	2 cut(s)	178, 2925
Eam1104I	CTCTTC	8 cut(s)	230, 540, 680, 1917, 2073, 2156, 2271, 3047
Eam1105I	GACNNNNNGTC	1 cut(s)	355
EarI	CTCTTC	8 cut(s)	230, 540, 680, 1917, 2073, 2156, 2271, 3047
EciI	GGCGGA	1 cut(s)	3201
Ecl136II	GAGCTC	2 cut(s)	43, 517
Eco130I	CCWWGG	8 cut(s)	163, 219, 397, 1558, 1623, 2268, 2865, 3334
Eco147I	AGGCCT	2 cut(s)	2773, 3241
Eco24I	GRGCYC	3 cut(s)	45, 519, 1989
Eco32I	GATATC	1 cut(s)	2014
Eco47I	GGWCC	4 cut(s)	192, 205, 2053, 2869
Eco47III	AGCGCT	2 cut(s)	1541, 3158
Eco53kI	GAGCTC	2 cut(s)	43, 517
Eco57I	CTGAAG	9 cut(s)	221, 582, 1454, 1496, 1907, 2129, 2295, 3381, 3444
Eco81I	CCTNAGG	1 cut(s)	471
Eco88I	CYCGRG	1 cut(s)	44
EcoICRI	GAGCTC	2 cut(s)	43, 517
EcoO109I	RGGNCCY	2 cut(s)	192, 205
EcoRI	GAATTC	1 cut(s)	402
EcoRII	CCWGG	6 cut(s)	18, 72, 1047, 1508, 3069, 3341
EcoRV	GATATC	1 cut(s)	2014
EcoT14I	CCWWGG	8 cut(s)	163, 219, 397, 1558, 1623, 2268, 2865, 3334
EcoT38I	GRGCYC	3 cut(s)	45, 519, 1989
ErhI	CCWWGG	8 cut(s)	163, 219, 397, 1558, 1623, 2268, 2865, 3334
FalI	AAGNNNNNCTT	6 cut(s)	561, 593, 1239, 1271, 2268, 2300
FaqI	GGGAC	5 cut(s)	178, 379, 2357, 3091, 3461
FauI	CCCGC	2 cut(s)	2538, 2775
FblI	GTMKAC	1 cut(s)	895
FokI	GGATG	9 cut(s)	1031, 1169, 1177, 1652, 2831, 3011, 3051, 3275, 3302
FriOI	GRGCYC	3 cut(s)	45, 519, 1989
FspBI	CTAG	7 cut(s)	38, 321, 407, 794, 1067, 1919, 2324
GlaI	GCGC	3 cut(s)	1541, 1868, 3158
GsaI	CCCAGC	2 cut(s)	199, 1164
GsuI	CTGGAG	2 cut(s)	1897, 2931
HaeII	RGCGCY	3 cut(s)	1543, 1870, 3160
HapII	CCGG	4 cut(s)	64, 126, 177, 327
HgaI	GACGC	2 cut(s)	155, 3458
HhaI	GCGC	3 cut(s)	1542, 1869, 3159
Hin1I	GRCGYC	1 cut(s)	147
Hin6I	GCGC	3 cut(s)	1540, 1867, 3157
HinP1I	GCGC	3 cut(s)	1540, 1867, 3157
HincII	GTYRAC	1 cut(s)	2182
HindII	GTYRAC	1 cut(s)	2182
HindIII	AAGCTT	4 cut(s)	154, 372, 386, 2153
HpaII	CCGG	4 cut(s)	64, 126, 177, 327
HphI	GGTGA	2 cut(s)	452, 1112
HpyCH4III	ACNGT	7 cut(s)	230, 1097, 1263, 1618, 1905, 3199, 3219
HpyCH4IV	ACGT	1 cut(s)	1468
HpySE526I	ACGT	1 cut(s)	1468
Hsp92I	GRCGYC	1 cut(s)	147
HspAI	GCGC	3 cut(s)	1540, 1867, 3157
KflI	GGGWCCC	1 cut(s)	192
LguI	GCTCTTC	3 cut(s)	230, 1917, 3047
LmnI	GCTCC	9 cut(s)	128, 189, 329, 979, 1276, 1915, 2136, 2854, 3338
Lsp1109I	GCAGC	8 cut(s)	584, 906, 1020, 1960, 2453, 2629, 3215, 3262
LweI	GCATC	8 cut(s)	1053, 1147, 1380, 1630, 1935, 2809, 2989, 3262
MabI	ACCWGGT	1 cut(s)	72
MaeI	CTAG	7 cut(s)	38, 321, 407, 794, 1067, 1919, 2324
MaeII	ACGT	1 cut(s)	1468
MaeIII	GTNAC	5 cut(s)	1612, 1769, 1844, 2384, 2905
MbiI	CCGCTC	1 cut(s)	2782
MflI	RGATCY	3 cut(s)	297, 498, 2689
MhlI	GDGCHC	4 cut(s)	45, 519, 1989, 2141
MlsI	TGGCCA	1 cut(s)	2927
MluNI	TGGCCA	1 cut(s)	2927
MlyI	GAGTC	6 cut(s)	299, 458, 1525, 1967, 2181, 2759
MmeI	TCCRAC	4 cut(s)	117, 159, 1893, 2487
Mox20I	TGGCCA	1 cut(s)	2927
MroXI	GAANNNNTTC	4 cut(s)	952, 1140, 1254, 2409
MscI	TGGCCA	1 cut(s)	2927
MslI	CAYNNNNRTG	3 cut(s)	1407, 2491, 3140
Msp20I	TGGCCA	1 cut(s)	2927
MspA1I	CMGCKG	2 cut(s)	199, 1970
MspCI	CTTAAG	1 cut(s)	389
MspI	CCGG	4 cut(s)	64, 126, 177, 327
MspR9I	CCNGG	7 cut(s)	20, 65, 74, 1049, 1510, 3071, 3343
Mva1269I	GAATGC	1 cut(s)	1414
MvaI	CCWGG	6 cut(s)	20, 74, 1049, 1510, 3071, 3343
MvnI	CGCG	2 cut(s)	774, 2232
NciI	CCSGG	1 cut(s)	65
NcoI	CCATGG	5 cut(s)	219, 1558, 1623, 2268, 2865
NlaIV	GGNNCC	7 cut(s)	68, 85, 115, 124, 193, 194, 3340
NmeAIII	GCCGAG	1 cut(s)	1115
NmuCI	GTSAC	2 cut(s)	1612, 1844
NruI	TCGCGA	1 cut(s)	2232
NspI	RCATGY	2 cut(s)	1220, 1388
PaeI	GCATGC	1 cut(s)	1220
PaeR7I	CTCGAG	1 cut(s)	44
PasI	CCCWGGG	2 cut(s)	19, 3070
PceI	AGGCCT	2 cut(s)	2773, 3241
PciSI	GCTCTTC	3 cut(s)	230, 1917, 3047
PcsI	WCGNNNNNNNCGW	2 cut(s)	222, 3316
PctI	GAATGC	1 cut(s)	1414
PdmI	GAANNNNTTC	4 cut(s)	952, 1140, 1254, 2409
PfeI	GAWTC	7 cut(s)	1289, 1358, 1505, 2464, 2603, 2705, 3312
PflMI	CCANNNNNTGG	1 cut(s)	2269
PfoI	TCCNGGA	1 cut(s)	1047
PleI	GAGTC	6 cut(s)	298, 457, 1525, 1966, 2181, 2758
PpsI	GAGTC	6 cut(s)	298, 457, 1525, 1966, 2181, 2758
Ppu21I	YACGTR	1 cut(s)	1469
PpuMI	RGGWCCY	2 cut(s)	192, 205
PsiI	TTATAA	1 cut(s)	378
Psp124BI	GAGCTC	2 cut(s)	45, 519
Psp5II	RGGWCCY	2 cut(s)	192, 205
Psp6I	CCWGG	6 cut(s)	18, 72, 1047, 1508, 3069, 3341
PspFI	CCCAGC	2 cut(s)	195, 1160
PspGI	CCWGG	6 cut(s)	18, 72, 1047, 1508, 3069, 3341
PspN4I	GGNNCC	7 cut(s)	68, 85, 115, 124, 193, 194, 3340
PspPI	GGNCC	9 cut(s)	67, 83, 113, 192, 205, 312, 2053, 2720, 2869
PspPPI	RGGWCCY	2 cut(s)	192, 205
PstI	CTGCAG	2 cut(s)	1038, 2443
PstNI	CAGNNNCTG	1 cut(s)	509
PsuI	RGATCY	3 cut(s)	297, 498, 2689
PvuII	CAGCTG	2 cut(s)	199, 1970
RruI	TCGCGA	1 cut(s)	2232
RsaI	GTAC	3 cut(s)	341, 1783, 3134
RsaNI	GTAC	3 cut(s)	340, 1782, 3133
RseI	CAYNNNNRTG	3 cut(s)	1407, 2491, 3140
SacI	GAGCTC	2 cut(s)	45, 519
SapI	GCTCTTC	3 cut(s)	230, 1917, 3047
Sau96I	GGNCC	9 cut(s)	67, 83, 113, 192, 205, 312, 2053, 2720, 2869
SchI	GAGTC	6 cut(s)	299, 458, 1525, 1967, 2181, 2759
ScrFI	CCNGG	7 cut(s)	20, 65, 74, 1049, 1510, 3071, 3343
SduI	GDGCHC	4 cut(s)	45, 519, 1989, 2141
SexAI	ACCWGGT	1 cut(s)	72
SfaNI	GCATC	8 cut(s)	1053, 1147, 1380, 1630, 1935, 2809, 2989, 3262
SfcI	CTRYAG	6 cut(s)	1034, 1579, 2124, 2439, 2660, 3235
Sfr274I	CTCGAG	1 cut(s)	44
SinI	GGWCC	4 cut(s)	192, 205, 2053, 2869
SlaI	CTCGAG	1 cut(s)	44
SmiMI	CAYNNNNRTG	3 cut(s)	1407, 2491, 3140
SmlI	CTYRAG	7 cut(s)	44, 389, 491, 518, 1535, 2156, 2252
SmoI	CTYRAG	7 cut(s)	44, 389, 491, 518, 1535, 2156, 2252
SphI	GCATGC	1 cut(s)	1220
SseBI	AGGCCT	2 cut(s)	2773, 3241
SspI	AATATT	2 cut(s)	1139, 3025
SspMI	CTAG	7 cut(s)	38, 321, 407, 794, 1067, 1919, 2324
SstI	GAGCTC	2 cut(s)	45, 519
StuI	AGGCCT	2 cut(s)	2773, 3241
StyD4I	CCNGG	7 cut(s)	18, 63, 72, 1047, 1508, 3069, 3341
StyI	CCWWGG	8 cut(s)	163, 219, 397, 1558, 1623, 2268, 2865, 3334
TaaI	ACNGT	7 cut(s)	230, 1097, 1263, 1618, 1905, 3199, 3219
TaiI	ACGT	1 cut(s)	1471
TaqI	TCGA	9 cut(s)	45, 447, 946, 1989, 2016, 2584, 2629, 3163, 3310
TatI	WGTACW	1 cut(s)	3132
TauI	GCSGC	3 cut(s)	252, 333, 774
TfiI	GAWTC	7 cut(s)	1289, 1358, 1505, 2464, 2603, 2705, 3312
TscAI	CASTG	7 cut(s)	235, 340, 487, 1102, 1530, 1621, 3285
TseFI	GTSAC	2 cut(s)	1612, 1844
TseI	GCWGC	8 cut(s)	572, 919, 1033, 1948, 2441, 2617, 3203, 3275
Tsp45I	GTSAC	2 cut(s)	1612, 1844
TspGWI	ACGGA	5 cut(s)	176, 992, 2045, 3102, 3308
TspRI	CASTG	7 cut(s)	235, 340, 487, 1102, 1530, 1621, 3285
Van91I	CCANNNNNTGG	1 cut(s)	2269
Vha464I	CTTAAG	1 cut(s)	389
VpaK11BI	GGWCC	4 cut(s)	192, 205, 2053, 2869
XceI	RCATGY	2 cut(s)	1220, 1388
XhoI	CTCGAG	1 cut(s)	44
XmiI	GTMKAC	1 cut(s)	895
XmnI	GAANNNNTTC	4 cut(s)	952, 1140, 1254, 2409
XspI	CTAG	7 cut(s)	38, 321, 407, 794, 1067, 1919, 2324

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.