MD10G1183700.v1.1

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006326
Physical Location & Seq
Forward (+)
27676903 .. 27686376
9474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1183700.v1.1.491

Sequence Viewer

Length: 3708 bp
ATGCCGTCCCTGGTGTCTCAGGGAAAAATCCTCCGCCTGGAGCTCGAGAACTTCAAGTCCTATAAGGGCTTCCAGACCATCGGCCCCTTCTACGACTTCACGGCGATTATTGGGCCCAATGGCGCTGGAAAGTCCAACCTCATGGACGCCATAAGCTTCGTCCTCGGCGTGCGGACGGGCCACCTCCGTGGGGCCCAGCTGAAGGACCTGATCTATGCCTTCGACGACAAGGAGAAGGACCAGAAGGGCCGCAGGGCCTTTGTCCGCTTGGTTTACCAGCTGGACAACGGGTCGGAGCTCCAGTTCACCCGAGCCATTACAGGCGGAGCGGGCAGCGAGTACCGTATCGATGGGGCGTCTGTGACTTGGGAAGAGTACAATGCGAAGCTCAGGTCTCTCGGGATTCTTGTCAAGGCCCGGAATTTCCTCGTCTTTCAGGGTGATGTGGAGTCCATTGCCTCCAAAAATCCCAAGGAACTCACTGCACTTCTTGAGCAGATCTCTGGGTCTGATGAACTCAAGAGAGACTATGAAAAATATGAAGAAGAAAAATCCGAAGCTGAACAAAAAGCAAGTCTTGTTTATCAGAGAAAGAGAACAATAGTATTGGAGAGGAGGCAAAAGAAAGAACAAAAAGAAGAAGCCGAAAAACACCTCCGCCTACAAGATCAACTGAAATCTTTGAAGAAAGAACATTCTTTATGGCAATTGTTCAACATAGAAAAGGATATTGCAAAAATGACTGAGGAACTTGAAGCTGAAAAGAAAAGCCGTGAACAAGTTATGCAAGAAATTGATGAATTTCAGCTAGAAGCAAACAATAAGAAGAAAGAACAAGCAAAATATCTGAAAGAGATTGCACAATGTGAAAAGAAGATTTCAGATAGAAATAATAAACTTGACAAAAGTCAACCAGACCTTCTGAAACTGAAAGAGGAAATGTCTCGTATAAATGCAAAAATCAAGAAAAGTGAGAAGGAGCTCGCTAAGAAAGAACAAGAAAGGAAAAGACATAAAGAGGATATAAAGCAGCTCCAGAAGGGCATACATGATTTGACTGCAAAACTAGAGGATTTACATGAAAAAGGCCGAGATAGTGGCGACAAACTGAAGTTAGATGATACTGAATTAAGGGAATATTTTCGAATCAAGGAAGATGCTGGAATGAAAACTGCAAAGCTAAGAGATGAGAAAGAGGTTCTGGATAGGCAACAACATGCTGATCTTGAAGCTCAAAAGAATTTGGAAGAAAATCTTCAACAGTTGAGAAGTCGCGAGGCTGAATTAGAGGAACAAGAGGAACAAATGCTAACAAGGCAGAAAAAAATTAAAGAAAATTCTGCGAAACATAGGGATGAAGCTAAAAATCTGGATAATGAACTGCGTGCCATGCAATCTAAAAATTCACAGGCCAGAACAAAATATGAAAATCTGAAGTCAAAAATTGATGAAATAGAAAAGCAACTACGGGAATTGAAGGCTGACAGATATGAGAATGAGAGAGATTCCAGATTGTCTCAGGCAGTCGAGACTCTCAAACGCCTGTTTCACGGTGTCCACGGTCGTATGACTGAACTTTGTAGGCCAACACAAAAAAAGTATAACCTCGCTGTTACTGTTGCCATGGGTAAATTTATGGATGCAGTTGTAGTTGAGGATGAACAAACGGGAAAGGAATGCATCAAGTCATCACTGGATTCATGGGTTGACAAAACTTTTGAAGTATGTGAATGCAATTCTGGTACTGATCCTTTTACATCCCTGCAGTATTTGAAAGAACAAAGGCTTCCTCCTCAGACATTCATACCTCTTCAGTCTGTTCGTGTGAAGCCCGTAATGGAGAGATTGCGCAATTTAGGTGGTACTGCAAAGCTGATCTTTGATGCAATTCTTTTTGCGGTTGGCAATACTCTTGTCTGTGATGAACTTGATGAGGCTAAGCGTCTCAGCTGGACTGGTGAGAGGTTCAAAGTTGTAACTGTTGATGGAATTTTGCTCGCGAAATCCGGCACAATGACTGGCGGTACCAGTGGTGGAATGGAAGCAAGGTCAAAACAGTGGGATGATAAAAAAGTTGAAGGACTGAAGAAGAAGAAAGAACAGTTTGAGTCAGAGTTGGAAGAGCTGGGGTCAATTAGAGAGATGCAGATAAAAGAGTCTGAGACAACTGGTAGAATTAGTGGACTTCAAAAAAAGATCCAGTATGCAGAGATTGAGAAGAAAAGCATTAAGGACAAACTTGCAACCTTGGCGCGGGAAAAGCAGAATATAAAAGAAGAGATTGATCGCAGCAGTCCTGAACTTCTCAAGTTAAAGCAAGCTGTAGATAAGCGAAGCAAGGAAATCAATAAGCTTGAGAAGAGGATAAATGAAATTGTTGACAGAATTTACAAAGATTTTAGTAGATCTGTTGGGGTGGCAAACATCCGTGAGTACGAGGAAAATCAACTCAAGGCCTCCCAATATATGGCTGAAGAGAGGCTTAGTTTGAGTAGCCAGCTTTCAAAGTTGAAGTACCAGTTAGAGTATGAGCAAAACCGGGACATGGAATCACGAATTAACGAACTCCAAGATTCTATTAGTAAATTTAAAAAAGCTTTAGAACGGGTTCAGAAGGAAGAGGCTGCAGCCAAGTCAGCAGCAGAAAAAGCTTCTGGTGAGATCGATCGTTGGAAGGAAGAAATACAAGAATGGAAATCCAAGTCAGAAGGGTGTGAGAAGGAAATCCAAGAATGGAACAAGCGGGGTTCTACAGCTACAACAAGTTTATCCAAACTTAATCGACAGATACATGCTAAGGAGTCACAGATTGAGCAGCTGATGTCACAGAAGCAGGAAATAGTAGAGAAATGTGAATTGGAACAAATAAACCTTCCCATCATCTCAGACCCAATGGAGACTGATTCCTCCACAATGGGCCCATGTTTTGATTTTAGTCAGTTAAACAAGTCTCATGTGCGGGACAGAAGGCCATCTGAACGGGAGAAGCTTGAGGTGGAATTTAAGCAAAAAATGGATGCCGTAATTTCAGAAATTGAAAAAACAGCTCCAAATTTGAAGGCCCTGGACCAATATGAGGCTATTAAAGAAAAGGAAAGAGCTGTAACTGAAGAGTTTGAAGTGGCTAGAATAGAAGAGAAGGAAAAAGCTGATCTGTTTAACTCAGTTAAGCAGAAGAGGTACGAGTTGTTTATGGATGCCTTCCAACATATTTCCAGTAGTATTGATAAGATATACAAACAACTCACGAAGAGCAACACACATCCACTGGGTGGGACGGCATACTTAAATTTGGAAAATGAAGATGATCCATTTTTACATGGCATCAAGTACACTGCTATGCCCCCAACAAAGCGCTTTCGTGATATGGAACAATTATCTGGCGGCGAGAAAACCGTTGCTGCATTGGCATTACTCTTTTCCATACACAGTTTTAGGCCTTCACCGTTTTTCATACTGGATGAAGTCGATGCTGCACTAGATAACTTGAATGTTGCTAAGGTTGCTGGATTCATCCGTTCAAAGTCACGTGAAGGAGCCAGGGCTAACCAAGATGATGAGGGAGGAGGTGGTTTTCAGAGCATTGTAATTTCTCTAAAAGATAGCTTCTACGACAAGGCTGATGCTTTAGTTGGGGTTTACAGGGACTGCGAAAGGAGCTGTTCGGAAACTCTGACATTTGACCTGACCAAGTATCGGGAGTCGTGA

Protein Analysis

1236

Amino Acids

142.46

Weight (kDa)

6.85

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_15 PF13175 9 - 347 2.2e-14 AAA ATPase domain
SMC_N PF02463 10 - 1215 1.5e-38 RecF/RecN/SMC N terminal domain
AAA_23 PF13476 12 - 231 2.5e-16 AAA domain
AAA_29 PF13555 12 - 55 7.7e-07 P-loop containing region of AAA domain
AAA_21 PF13304 33 - 62 4.3e-07 AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge PF06470 516 - 562 6.1e-08 SMC proteins Flexible Hinge Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1850
Acc65I GGTACC 1 cut(s) 2024
AccB1I GGYRCC 1 cut(s) 2024
AccB7I CCANNNNNTGG 2 cut(s) 2467, 3272
AccBSI CCGCTC 1 cut(s) 329
AccII CGCG 3 cut(s) 1275, 2000, 2254
AclWI GGATC 3 cut(s) 1742, 2191, 3302
AcuI CTGAAG 7 cut(s) 221, 1130, 1454, 1796, 2105, 2493, 3129
AcvI CACGTG 1 cut(s) 3530
AcyI GRCGYC 2 cut(s) 147, 356
AdeI CACNNNGTG 2 cut(s) 866, 3272
AfaI GTAC 9 cut(s) 341, 377, 1744, 1864, 2026, 2435, 2516, 3182, 3332
AfeI AGCGCT 1 cut(s) 3356
AfiI CCNNNNNNNGG 9 cut(s) 37, 190, 202, 2253, 2467, 2545, 3076, 3272, 3696
AhdI GACNNNNNGTC 1 cut(s) 289
AjnI CCWGG 4 cut(s) 9, 36, 3063, 3539
AjuI GAANNNNNNNTTGG 2 cut(s) 2840, 2872
AleI CACNNNNGTG 1 cut(s) 186
AloI GAACNNNNNNTCC 4 cut(s) 41, 73, 287, 319
Alw21I GWGCWC 3 cut(s) 45, 300, 984
AlwI GGATC 3 cut(s) 1742, 2191, 3302
AlwNI CAGNNNCTG 1 cut(s) 3648
Ama87I CYCGRG 3 cut(s) 44, 309, 398
Aor51HI AGCGCT 1 cut(s) 3356
ApaI GGGCCC 3 cut(s) 117, 196, 2921
ApeKI GCWGC 9 cut(s) 333, 1030, 2289, 2624, 2627, 2639, 2815, 3401, 3473
ArsI GACNNNNNNTTYG 6 cut(s) 377, 409, 559, 591, 1700, 1732
Asp700I GAANNNNTTC 3 cut(s) 1140, 1254, 2607
Asp718I GGTACC 1 cut(s) 2024
AspLEI GCGC 4 cut(s) 125, 1851, 2254, 3357
AsuC2I CCSGG 2 cut(s) 418, 2540
AsuHPI GGTGA 5 cut(s) 298, 452, 1970, 2669, 3435
AsuII TTCGAA 1 cut(s) 1144
AvaI CYCGRG 3 cut(s) 44, 309, 398
AvaII GGWCC 3 cut(s) 205, 238, 3067
BaeGI GKGCMC 3 cut(s) 117, 196, 2921
BanI GGYRCC 1 cut(s) 2024
BanII GRGCYC 6 cut(s) 45, 117, 196, 300, 984, 2921
BbrPI CACGTG 1 cut(s) 3530
Bbv12I GWGCWC 3 cut(s) 45, 300, 984
BbvI GCAGC 9 cut(s) 345, 1042, 2301, 2611, 2639, 2651, 2827, 3388, 3460
BccI CCATC 5 cut(s) 86, 344, 1979, 2885, 2980
BceAI ACGGC 4 cut(s) 117, 756, 3005, 3294
BciT130I CCWGG 4 cut(s) 11, 38, 3065, 3541
BcnI CCSGG 2 cut(s) 418, 2540
BfaI CTAG 4 cut(s) 809, 1067, 3126, 3479
BfmI CTRYAG 4 cut(s) 1763, 2322, 2625, 2751
BfoI RGCGCY 2 cut(s) 126, 3358
BglII AGATCT 2 cut(s) 498, 2405
BlpI GCTNAGC 1 cut(s) 1938
Bme1390I CCNGG 6 cut(s) 11, 38, 418, 2540, 3065, 3541
Bme18I GGWCC 3 cut(s) 205, 238, 3067
BmeRI GACNNNNNGTC 1 cut(s) 289
BmeT110I CYCGRG 3 cut(s) 44, 309, 398
BmiI GGNNCC 7 cut(s) 85, 115, 193, 194, 2026, 2919, 3538
BmrFI CCNGG 6 cut(s) 11, 38, 418, 2540, 3065, 3541
BmrI ACTGGG 1 cut(s) 3278
BmuI ACTGGG 1 cut(s) 3278
BoxI GACNNNNGTC 1 cut(s) 906
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 3 cut(s) 59, 284, 1019
Bpu10I CCTNAGC 3 cut(s) 389, 2796, 3498
Bpu1102I GCTNAGC 1 cut(s) 1938
Bpu14I TTCGAA 1 cut(s) 1144
BpuEI CTTGAG 6 cut(s) 503, 512, 2291, 2375, 2435, 3011
BpuMI CCSGG 2 cut(s) 418, 2540
Bsa29I ATCGAT 2 cut(s) 348, 2664
BsaAI YACGTR 1 cut(s) 3530
BsaHI GRCGYC 2 cut(s) 147, 356
BsaI GGTCTC 1 cut(s) 399
BsaJI CCNNGG 9 cut(s) 9, 163, 187, 471, 1558, 1623, 2247, 3063, 3540
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 9 cut(s) 37, 190, 202, 2253, 2467, 2545, 3076, 3272, 3696
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 4 cut(s) 11, 38, 3065, 3541
BseCI ATCGAT 2 cut(s) 348, 2664
BseDI CCNNGG 9 cut(s) 9, 163, 187, 471, 1558, 1623, 2247, 3063, 3540
BseLI CCNNNNNNNGG 9 cut(s) 37, 190, 202, 2253, 2467, 2545, 3076, 3272, 3696
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 9 cut(s) 32, 403, 735, 1532, 1808, 1960, 2151, 2898, 3177
BseRI GAGGAG 3 cut(s) 628, 1782, 3579
BseSI GKGCMC 3 cut(s) 117, 196, 2921
BseXI GCAGC 9 cut(s) 345, 1042, 2301, 2611, 2639, 2651, 2827, 3388, 3460
BseYI CCCAGC 2 cut(s) 195, 2125
BsgI GTGCAG 2 cut(s) 468, 3459
Bsh1236I CGCG 3 cut(s) 1275, 2000, 2254
Bsh1285I CGRYCG 2 cut(s) 1564, 2668
BshNI GGYRCC 1 cut(s) 2024
BshVI ATCGAT 2 cut(s) 348, 2664
BsiEI CGRYCG 2 cut(s) 1564, 2668
BsiHKAI GWGCWC 3 cut(s) 45, 300, 984
BsiHKCI CYCGRG 3 cut(s) 44, 309, 398
BsiSI CCGG 3 cut(s) 418, 2007, 2539
BslFI GGGAC 4 cut(s) 2555, 2975, 3289, 3659
BslI CCNNNNNNNGG 9 cut(s) 37, 190, 202, 2253, 2467, 2545, 3076, 3272, 3696
BsmBI CGTCTC 1 cut(s) 1949
BsmFI GGGAC 4 cut(s) 2555, 2975, 3289, 3659
BsmI GAATGC 2 cut(s) 1682, 1736
Bso31I GGTCTC 1 cut(s) 399
BsoBI CYCGRG 3 cut(s) 44, 309, 398
Bsp119I TTCGAA 1 cut(s) 1144
Bsp120I GGGCCC 3 cut(s) 113, 192, 2917
Bsp1286I GDGCHC 6 cut(s) 45, 117, 196, 300, 984, 2921
Bsp1720I GCTNAGC 1 cut(s) 1938
Bsp19I CCATGG 1 cut(s) 1623
Bsp68I TCGCGA 2 cut(s) 1275, 2000
BspCNI CTCAG 9 cut(s) 31, 402, 736, 1531, 1807, 1959, 2152, 2897, 3176
BspDI ATCGAT 2 cut(s) 348, 2664
BspFNI CGCG 3 cut(s) 1275, 2000, 2254
BspLI GGNNCC 7 cut(s) 85, 115, 193, 194, 2026, 2919, 3538
BspMAI CTGCAG 2 cut(s) 1767, 2629
BspPI GGATC 3 cut(s) 1742, 2191, 3302
BspQI GCTCTTC 2 cut(s) 2115, 3245
BspT104I TTCGAA 1 cut(s) 1144
BspT107I GGYRCC 1 cut(s) 2024
BspTNI GGTCTC 1 cut(s) 399
BsrBI CCGCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 453
BssECI CCNNGG 9 cut(s) 9, 163, 187, 471, 1558, 1623, 2247, 3063, 3540
BssNI GRCGYC 2 cut(s) 147, 356
BssT1I CCWWGG 3 cut(s) 471, 1623, 2247
Bst2UI CCWGG 4 cut(s) 11, 38, 3065, 3541
BstACI GRCGYC 2 cut(s) 147, 356
BstBAI YACGTR 1 cut(s) 3530
BstBI TTCGAA 1 cut(s) 1144
BstC8I GCNNGC 7 cut(s) 170, 331, 984, 1386, 1998, 2319, 2498
BstDSI CCRYGG 3 cut(s) 187, 1558, 1623
BstFNI CGCG 3 cut(s) 1275, 2000, 2254
BstH2I RGCGCY 2 cut(s) 126, 3358
BstHHI GCGC 4 cut(s) 125, 1851, 2254, 3357
BstMCI CGRYCG 2 cut(s) 1564, 2668
BstNI CCWGG 4 cut(s) 11, 38, 3065, 3541
BstNSI RCATGY 2 cut(s) 1220, 2795
BstPAI GACNNNNGTC 1 cut(s) 906
BstSCI CCNGG 6 cut(s) 9, 36, 416, 2538, 3063, 3539
BstSFI CTRYAG 4 cut(s) 1763, 2322, 2625, 2751
BstSLI GKGCMC 3 cut(s) 117, 196, 2921
BstUI CGCG 3 cut(s) 1275, 2000, 2254
BstV1I GCAGC 9 cut(s) 345, 1042, 2301, 2611, 2639, 2651, 2827, 3388, 3460
BstX2I RGATCY 3 cut(s) 498, 2196, 2405
BstXI CCANNNNNNTGG 2 cut(s) 142, 188
BstYI RGATCY 3 cut(s) 498, 2196, 2405
Bsu15I ATCGAT 2 cut(s) 348, 2664
BsuTUI ATCGAT 2 cut(s) 348, 2664
BtgI CCRYGG 3 cut(s) 187, 1558, 1623
BtsI GCAGTG 2 cut(s) 480, 3333
BtsIMutI CAGTG 6 cut(s) 480, 1691, 2035, 2063, 3266, 3333
BtuMI TCGCGA 2 cut(s) 1275, 2000
Cac8I GCNNGC 7 cut(s) 170, 331, 984, 1386, 1998, 2319, 2498
CaiI CAGNNNCTG 1 cut(s) 3648
CfoI GCGC 4 cut(s) 125, 1851, 2254, 3357
ClaI ATCGAT 2 cut(s) 348, 2664
CseI GACGC 3 cut(s) 155, 345, 1931
Csp6I GTAC 9 cut(s) 340, 376, 1743, 1863, 2025, 2434, 2515, 3181, 3331
CspCI CAANNNNNGTGG 2 cut(s) 1840, 1875
CviQI GTAC 9 cut(s) 340, 376, 1743, 1863, 2025, 2434, 2515, 3181, 3331
DraI TTTAAA 1 cut(s) 2590
DraIII CACNNNGTG 2 cut(s) 866, 3272
DriI GACNNNNNGTC 1 cut(s) 289
Eam1105I GACNNNNNGTC 1 cut(s) 289
EciI GGCGGA 3 cut(s) 23, 339, 647
Ecl136II GAGCTC 3 cut(s) 43, 298, 982
Eco130I CCWWGG 3 cut(s) 471, 1623, 2247
Eco147I AGGCCT 2 cut(s) 2456, 3439
Eco24I GRGCYC 6 cut(s) 45, 117, 196, 300, 984, 2921
Eco31I GGTCTC 1 cut(s) 399
Eco47I GGWCC 3 cut(s) 205, 238, 3067
Eco47III AGCGCT 1 cut(s) 3356
Eco53kI GAGCTC 3 cut(s) 43, 298, 982
Eco57I CTGAAG 7 cut(s) 221, 1130, 1454, 1796, 2105, 2493, 3129
Eco72I CACGTG 1 cut(s) 3530
Eco88I CYCGRG 3 cut(s) 44, 309, 398
EcoICRI GAGCTC 3 cut(s) 43, 298, 982
EcoO109I RGGNCCY 4 cut(s) 192, 205, 255, 3061
EcoRII CCWGG 4 cut(s) 9, 36, 3063, 3539
EcoT14I CCWWGG 3 cut(s) 471, 1623, 2247
EcoT22I ATGCAT 1 cut(s) 1682
EcoT38I GRGCYC 6 cut(s) 45, 117, 196, 300, 984, 2921
ErhI CCWWGG 3 cut(s) 471, 1623, 2247
Esp3I CGTCTC 1 cut(s) 1949
FalI AAGNNNNNCTT 8 cut(s) 561, 593, 1239, 1271, 1862, 1894, 2466, 2498
FaqI GGGAC 4 cut(s) 2555, 2975, 3289, 3659
FauI CCCGC 4 cut(s) 322, 2247, 2736, 2952
FriOI GRGCYC 6 cut(s) 45, 117, 196, 300, 984, 2921
FspBI CTAG 4 cut(s) 809, 1067, 3126, 3479
FspI TGCGCA 1 cut(s) 1850
GlaI GCGC 4 cut(s) 124, 1850, 2253, 3356
GsaI CCCAGC 2 cut(s) 199, 2129
GsuI CTGGAG 3 cut(s) 59, 284, 1019
HaeII RGCGCY 2 cut(s) 126, 3358
HapII CCGG 3 cut(s) 418, 2007, 2539
HgaI GACGC 3 cut(s) 155, 345, 1931
HhaI GCGC 4 cut(s) 125, 1851, 2254, 3357
Hin1I GRCGYC 2 cut(s) 147, 356
Hin6I GCGC 4 cut(s) 123, 1849, 2252, 3355
HinP1I GCGC 4 cut(s) 123, 1849, 2252, 3355
HincII GTYRAC 3 cut(s) 911, 1708, 2380
HindII GTYRAC 3 cut(s) 911, 1708, 2380
HindIII AAGCTT 5 cut(s) 154, 2351, 2595, 2649, 2987
HpaII CCGG 3 cut(s) 418, 2007, 2539
HphI GGTGA 5 cut(s) 298, 452, 1970, 2669, 3435
Hpy99I CGWCG 1 cut(s) 227
HpyCH4IV ACGT 1 cut(s) 3529
HpySE526I ACGT 1 cut(s) 3529
Hsp92I GRCGYC 2 cut(s) 147, 356
HspAI GCGC 4 cut(s) 123, 1849, 2252, 3355
KpnI GGTACC 1 cut(s) 2028
LguI GCTCTTC 2 cut(s) 2115, 3245
LmnI GCTCC 9 cut(s) 40, 295, 303, 326, 979, 1038, 3052, 3536, 3657
Lsp1109I GCAGC 9 cut(s) 345, 1042, 2301, 2611, 2639, 2651, 2827, 3388, 3460
MaeI CTAG 4 cut(s) 809, 1067, 3126, 3479
MaeII ACGT 1 cut(s) 3529
MaeIII GTNAC 7 cut(s) 361, 1612, 1975, 2802, 2823, 3103, 3525
MbiI CCGCTC 1 cut(s) 329
MfeI CAATTG 1 cut(s) 707
MflI RGATCY 3 cut(s) 498, 2196, 2405
MhlI GDGCHC 6 cut(s) 45, 117, 196, 300, 984, 2921
MlyI GAGTC 5 cut(s) 458, 1525, 2117, 2165, 2810
MmeI TCCRAC 5 cut(s) 159, 273, 2097, 2651, 3229
Mph1103I ATGCAT 1 cut(s) 1682
MroXI GAANNNNTTC 3 cut(s) 1140, 1254, 2607
MslI CAYNNNNRTG 3 cut(s) 186, 1353, 3338
MspA1I CMGCKG 4 cut(s) 199, 280, 1950, 2818
MspI CCGG 3 cut(s) 418, 2007, 2539
MspR9I CCNGG 6 cut(s) 11, 38, 418, 2540, 3065, 3541
MunI CAATTG 1 cut(s) 707
Mva1269I GAATGC 2 cut(s) 1682, 1736
MvaI CCWGG 4 cut(s) 11, 38, 3065, 3541
MvnI CGCG 3 cut(s) 1275, 2000, 2254
NciI CCSGG 2 cut(s) 418, 2540
NcoI CCATGG 1 cut(s) 1623
NlaIV GGNNCC 7 cut(s) 85, 115, 193, 194, 2026, 2919, 3538
NmeAIII GCCGAG 2 cut(s) 144, 1115
NmuCI GTSAC 4 cut(s) 361, 2802, 2823, 3525
NruI TCGCGA 2 cut(s) 1275, 2000
NsbI TGCGCA 1 cut(s) 1850
NsiI ATGCAT 1 cut(s) 1682
NspI RCATGY 2 cut(s) 1220, 2795
NspV TTCGAA 1 cut(s) 1144
OliI CACNNNNGTG 1 cut(s) 186
PaeR7I CTCGAG 1 cut(s) 44
PceI AGGCCT 2 cut(s) 2456, 3439
PciSI GCTCTTC 2 cut(s) 2115, 3245
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 2 cut(s) 1682, 1736
PdmI GAANNNNTTC 3 cut(s) 1140, 1254, 2607
PfeI GAWTC 8 cut(s) 403, 1146, 1505, 1697, 2549, 2573, 2903, 3510
PflMI CCANNNNNTGG 2 cut(s) 2467, 3272
Ple19I CGATCG 1 cut(s) 2668
PleI GAGTC 5 cut(s) 457, 1525, 2116, 2164, 2809
PmaCI CACGTG 1 cut(s) 3530
PmlI CACGTG 1 cut(s) 3530
PpsI GAGTC 5 cut(s) 457, 1525, 2116, 2164, 2809
Ppu21I YACGTR 1 cut(s) 3530
PpuMI RGGWCCY 1 cut(s) 205
PshAI GACNNNNGTC 1 cut(s) 906
Psp124BI GAGCTC 3 cut(s) 45, 300, 984
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 4 cut(s) 9, 36, 3063, 3539
PspCI CACGTG 1 cut(s) 3530
PspFI CCCAGC 2 cut(s) 195, 2125
PspGI CCWGG 4 cut(s) 9, 36, 3063, 3539
PspN4I GGNNCC 7 cut(s) 85, 115, 193, 194, 2026, 2919, 3538
PspOMI GGGCCC 3 cut(s) 113, 192, 2917
PspPPI RGGWCCY 1 cut(s) 205
PstI CTGCAG 2 cut(s) 1767, 2629
PstNI CAGNNNCTG 1 cut(s) 3648
PsuI RGATCY 3 cut(s) 498, 2196, 2405
PvuI CGATCG 1 cut(s) 2668
PvuII CAGCTG 4 cut(s) 199, 280, 1950, 2818
RruI TCGCGA 2 cut(s) 1275, 2000
RsaI GTAC 9 cut(s) 341, 377, 1744, 1864, 2026, 2435, 2516, 3182, 3332
RsaNI GTAC 9 cut(s) 340, 376, 1743, 1863, 2025, 2434, 2515, 3181, 3331
RseI CAYNNNNRTG 3 cut(s) 186, 1353, 3338
SacI GAGCTC 3 cut(s) 45, 300, 984
SapI GCTCTTC 2 cut(s) 2115, 3245
SchI GAGTC 5 cut(s) 458, 1525, 2117, 2165, 2810
ScrFI CCNGG 6 cut(s) 11, 38, 418, 2540, 3065, 3541
SduI GDGCHC 6 cut(s) 45, 117, 196, 300, 984, 2921
SfcI CTRYAG 4 cut(s) 1763, 2322, 2625, 2751
Sfr274I CTCGAG 1 cut(s) 44
SfuI TTCGAA 1 cut(s) 1144
SinI GGWCC 3 cut(s) 205, 238, 3067
SlaI CTCGAG 1 cut(s) 44
SmiMI CAYNNNNRTG 3 cut(s) 186, 1353, 3338
SmlI CTYRAG 7 cut(s) 44, 491, 518, 2306, 2354, 2450, 2990
SmoI CTYRAG 7 cut(s) 44, 491, 518, 2306, 2354, 2450, 2990
SseBI AGGCCT 2 cut(s) 2456, 3439
SspI AATATT 1 cut(s) 1139
SspMI CTAG 4 cut(s) 809, 1067, 3126, 3479
SstI GAGCTC 3 cut(s) 45, 300, 984
StuI AGGCCT 2 cut(s) 2456, 3439
StyD4I CCNGG 6 cut(s) 9, 36, 416, 2538, 3063, 3539
StyI CCWWGG 3 cut(s) 471, 1623, 2247
TaiI ACGT 1 cut(s) 3532
TaqI TCGA 8 cut(s) 45, 222, 348, 1144, 1527, 2664, 2782, 3468
TatI WGTACW 2 cut(s) 375, 3330
TauI GCSGC 2 cut(s) 252, 3387
TfiI GAWTC 8 cut(s) 403, 1146, 1505, 1697, 2549, 2573, 2903, 3510
TscAI CASTG 6 cut(s) 487, 1698, 2035, 2063, 3273, 3340
TseFI GTSAC 4 cut(s) 361, 2802, 2823, 3525
TseI GCWGC 9 cut(s) 333, 1030, 2289, 2624, 2627, 2639, 2815, 3401, 3473
Tsp45I GTSAC 4 cut(s) 361, 2802, 2823, 3525
TspGWI ACGGA 3 cut(s) 176, 2417, 3506
TspRI CASTG 6 cut(s) 487, 1698, 2035, 2063, 3273, 3340
Van91I CCANNNNNTGG 2 cut(s) 2467, 3272
VpaK11BI GGWCC 3 cut(s) 205, 238, 3067
XceI RCATGY 2 cut(s) 1220, 2795
XcmI CCANNNNNNNNNTGG 1 cut(s) 2035
XhoI CTCGAG 1 cut(s) 44
XmnI GAANNNNTTC 3 cut(s) 1140, 1254, 2607
XspI CTAG 4 cut(s) 809, 1067, 3126, 3479
Zsp2I ATGCAT 1 cut(s) 1682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.